id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-293072-giakcaki	Xu, Wan-Xiang	A simpler and more cost-effective peptide biosynthetic method using the truncated GST as carrier for epitope mapping	2017-10-12		.txt	text/plain	5303	227	52	The protocol involves the following core steps: (i) design of plus and minus strands of DNA fragments encoding overlapping 8/18mer peptides; (ii) chemical synthesis of the designed DNA fragments; (iii) development of r-clones using pXXGST-3 vector expressing each 8/18mer peptide fused with truncated GST188 protein; (iv) screening r-clones by running the cell pellets from each induced clone on SDS-PAGE gel followed by sequencing of inserted DNA fragments for each verified r-clone; and (v) Western blotting with either monoclonal antibodies or polyclonal antibodies.	./cache/cord-293072-giakcaki.txt	./txt/cord-293072-giakcaki.txt