id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-292237-45hi4iz2	Calvo-Pinilla, Eva	Establishment of a Bluetongue Virus Infection Model in Mice that Are Deficient in the Alpha/Beta Interferon Receptor	2009-04-09		.txt	text/plain	4735	251	53	Furthermore, IFNAR((−/−)) adult mice immunized with a BTV-4 inactivated vaccine showed the induction of neutralizing antibodies against BTV-4 and complete protection against challenge with a lethal dose of this virus. All these data, and the presence of an otherwise intact immune system in these mice [20, 22] suggest that IFNAR (2/2) mice could be a good animal model to study BTV infections and to evaluate vaccine strategies against this virus. By contrast, IFNAR (2/2) mice were susceptible to BTV-4 infection (Fig. 1A) , showing disease symptoms characterized by ocular discharges and apathy starting at 48 h.p.i. Disease progression led to animal death within 60 h.p.i. The LD 50 value was obtained by i.v. inoculation with 10-fold dilutions of BTV-4, resulting in a LD 50 value of 10 2.6 PFU (Fig. 1B) . Infectious viral titers were analyzed in the blood of immunized and nonimmunized IFNAR (2/2) mice by plaque assay after intravenous infection with BTV-4 (Fig. 5C ).	./cache/cord-292237-45hi4iz2.txt	./txt/cord-292237-45hi4iz2.txt