id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-291104-6chpmgry	Leung, Danny T. M.	Osteopontin Fragments with Intact Thrombin-Sensitive Site Circulate in Cervical Cancer Patients	2016-08-05		.txt	text/plain	6269	313	58	The possibility that the target epitope recognized by the antibody probe in these assays was absent from the circulating OPN due to protein truncation was supported by gel fractionation of the OPN found in patients' plasma: 60–64 kDa fragments were found instead of the presumably full-length OPN (68 kDa) seen in healthy people. Similar results were found when the serum of these individuals was examined: the cancer patients (424 +/-121 ng/ mL) had significantly (P = 0.0002) higher levels of OPN than healthy subjects (314 +/-98 ng/ mL) (Fig 5B) , the assay sensitivity and specificity being 18% and 97%, respectively. The finding that the mAb 446 inhibition ELISA and the commercial OPN kit could not detect elevated OPN levels in the cancer patients suggested the possibility that the OPN present could be fragmented i.e. the target sites for the antibodies used in these assays could be missing, whereas, by virtue of the design of the mAb 659-based assay, the thrombin-sensitive site must be present.	./cache/cord-291104-6chpmgry.txt	./txt/cord-291104-6chpmgry.txt