id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-290539-8ak2tths	Cagno, Valeria	Novel broad spectrum virucidal molecules against enveloped viruses	2018-12-07		.txt	text/plain	5524	276	51	To further elucidate the mechanism of action we performed a virucidal assay in which 9d was incubated with the virus at 10 μM 5μM or 1μM concentration for different times (Fig 6A) or for 1h with serial dilutions of compound ( Fig 6B) ; subsequently, the mixture was titrated on cells and the viral titer was evaluated at dilutions at which the compound concentration was known not to be active in plaquing efficiency assays. The irreversibility of the mechanism was also tested with an assay in which the compound was incubated with the virus for 1h and subsequently the mixture has been diluted in drug free medium for additional 1, 2, 3 or 4 hours before the addition on cells (S2 Fig) .	./cache/cord-290539-8ak2tths.txt	./txt/cord-290539-8ak2tths.txt