id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-289093-si8btsab	Beard, Philippa M.	A Loss of Function Analysis of Host Factors Influencing Vaccinia virus Replication by RNA Interference	2014-06-05		.txt	text/plain	6578	310	49	To explore these interactions a functional high throughput small interfering RNA (siRNA) screen targeting 6719 druggable cellular genes was undertaken to identify host factors (HF) influencing the replication and spread of an eGFP-tagged VACV. Multiple components of the AMPK complex were found to act as pro-viral HFs, while several septins, a group of highly conserved GTP binding proteins with a role in sequestering intracellular bacteria, were identified as strong anti-viral VACV HFs. This screen has identified novel and previously unexplored roles for cellular factors in poxvirus replication. The methodology in the previously published VACV screens varied considerably; Mercer et al [32] measured the growth of a thymidine-kinase-deficient VACV (strain Western Reserve) after only 8 h of infection, thereby identifying cellular proteins involved in the initial stages of virus replication but excluding analysis of viral spread.	./cache/cord-289093-si8btsab.txt	./txt/cord-289093-si8btsab.txt