id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-280846-bbv6f5gf	Greninger, Alexander L.	A Metagenomic Analysis of Pandemic Influenza A (2009 H1N1) Infection in Patients from North America	2010-10-18		.txt	text/plain	8023	327	44	To determine whether a pan-viral microarray assay was capable of identifying novel 2009 H1N1 in the absence of a priori sequence information, we used the Virochip to comprehensively screen for viruses in 29 nasopharyngeal swab samples from individuals with influenza-like illness. To further characterize the metagenomics of 2009 H1N1 infection in humans, we labeled the 17 influenza samples positive for 2009 H1N1 by Virochip with distinct molecular barcodes and analyzed them by paired-end deep sequencing on three lanes of an Illumina Genome Analyzer IIx. After trimming reads to remove barcodes and exclude low-complexity or primer sequences, 11,427,212 high-quality 60-bp sequence reads were subjected to an iterative BLASTN analysis pipeline (Fig. 1B) . After stratifying by originating location and corresponding method of sample processing (pre-DNase and/or post-DNase treatment), the percentage of total reads aligning to influenza was linearly correlated with calculated viral titers by realtime quantitative RT-PCR for sites in the United States (California) and Canada but not in Mexico (Fig. 5A ).	./cache/cord-280846-bbv6f5gf.txt	./txt/cord-280846-bbv6f5gf.txt