id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-270681-6ayciihs	Bálint, Ádám	Comparative In Vivo Analysis of Recombinant Type II Feline Coronaviruses with Truncated and Completed ORF3 Region	2014-02-20		.txt	text/plain	4679	230	49	Our previous in vitro comparative study on a feline coronavirus (FCoV) pair, differing only in the intactness of their ORF3abc regions, showed that the truncated ORF3abc plays an important role in the efficient macrophage/monocyte tropism of type II feline infectious peritonitis virus (FIPV). In the present study using the parent FIPV DF-2 strain and its recombinant derivates we aimed to collect in vivo data how the completed ORF3abc alters virulence, virus shedding, viremia, viral load of organs and humoral immune response against type II FCoV. Shedding of FIPV DF-2 and PBFIPV-DF-2 was detected from day 3 p.i. to euthanasia of the PIP diseased animals at very low and variable amounts of an average value close to the detection limit of the genomic qRT-PCR (1.9610 1 FCoV RNA copies per ml fecal extract) ( Fig. 1) with undetectable virus replication using the subgenomic qRT-PCR assay (data not shown).	./cache/cord-270681-6ayciihs.txt	./txt/cord-270681-6ayciihs.txt