id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-260432-imslfm4l	Marshall, Jenika D.	THP-1 macrophage cholesterol efflux is impaired by palmitoleate through Akt activation	2020-05-21		.txt	text/plain	5851	287	47	Thus, we hypothesized that one or more specific fatty acids that exist within the total FFA component of lipoprotein hydrolysis products that are generated by LPL impair cholesterol efflux through the activation of Akt. To test this hypothesis, using THP-1 macrophages, we examined the activation of Akt in response to various FFA mixtures that contain the concentrations of FFA species that we previously reported to be found within LPL hydrolysis products from total lipoproteins [16] . Lastly, we examined the molecular species of phosphoinositides (PIPx) of THP-1 macrophages treated with palmitoleate, to determine if there were changes to select PIPx species that may contribute to a preferential activation of Akt. We previously showed using antibody arrays that the hydrolysis products liberated by LPL from total lipoproteins (ρ<1.21 g/ml), as well as the reconstituted total FFA component matching that liberated by LPL at a physiological concentration of 0.68 mM, significantly increased the phosphorylation of Akt after 30 minutes within THP-1 macrophages [16] .	./cache/cord-260432-imslfm4l.txt	./txt/cord-260432-imslfm4l.txt