id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003712-mafz21no	Perez Vidakovics, Maria Laura A.	Junín virus induces autophagy in human A549 cells	2019-06-19		.txt	text/plain	6151	333	47	We found that JUNV infection induces an increment of the LC3-II/LC3-I ratio, an accumulation of punctate pattern in RFP-LC3-transfected cells and the colocalisation of viral nucleoprotein and LC3 protein, suggesting autophagosome formation. Either with the early autophagy inhibitor (3-MA) or the late inhibitor (BAF), the treatment pre or post-infection resulted in reduced expression of N and lower viral titre as compared to JUNV-infected cells that received no treatment (NT) (Fig 4B-4D) . In parallel, experiments performed adding Rap post-infection resulted in a similar increase in viral yields of JUNV, which indicates that the activation of autophagy benefits both viral entry/uncoating and later steps as assembling and viral budding (Fig 4) . When compared to control cells, an increase in the number of mCherry puncta following rapamycin treatment was observed, indicating promoted autophagy and fusion between autophagosomes and acidic compartments (Fig 5C) .	./cache/cord-003712-mafz21no.txt	./txt/cord-003712-mafz21no.txt