id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003548-zuwt7gk5	Cai, Haiming	Modulating the 3’ end-DNA and the fermentation process for enhanced production and biological activity of porcine interferon-gamma	2019-03-26		.txt	text/plain	6848	447	56	In this study, we optimized the pIFN-γ gene recombination sequence and mutated the 3' end of the pIFN-γ gene, resulting in a higher expression level and stronger biological activity, as well as a significant upregulation in the expression of the interferon-stimulated genes Mx1 and OAS1 in IPEC-J2 jejunal epithelial cells. These data demonstrated, for the first time, that the expression level of pIFN-γ in Pichia pastoris was improved significantly by gene optimization with 3' end mutation and a fermentation process that maintained good biological activity, which is beneficial to the application of pIFN-γ in animal husbandry. The expression of pIFN-γ protein was maximal when the concentration of DO in the substrate was 30%, especially for the recombinant Pichia pastoris with the optimized pIFN-γ gene and the 3' signaling peptide mutation, which had significantly higher expression than that of the other treatment groups (p<0.05).	./cache/cord-003548-zuwt7gk5.txt	./txt/cord-003548-zuwt7gk5.txt