id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002624-59nznqsd	Ti, Jinfeng	Identification of one B-cell epitope from NS1 protein of duck Tembusu virus with monoclonal antibodies	2017-07-26		.txt	text/plain	4050	210	53	title: Identification of one B-cell epitope from NS1 protein of duck Tembusu virus with monoclonal antibodies This study describes the identification of one linear B-cell epitope on TMUV NS1 protein with monoclonal antibody (mAb) 3G2 by indirect enzyme-linked immunosorbent assay (ELISA). In this study, one linear B-cell epitope was identified and characterized with one monoclonal antibody against TMUV NS1 protein. To screen the antigenic epitope of TMUV NS1 mAb, 35 short peptide fusion proteins were successfully expressed in prokaryotic expression system and purified. A pane of 16-AA polypeptides of NS1 protein was expressed and one 16-AA polypeptide NS1-27 was screened and identified by 3G2 mAb. In order to accurately map the B-cell epitope, a set of truncated fusion oligopeptides of NS1-27 were expressed. The B-cell epitope of TMUV NS1 protein could apply into the development of detection methods to investigate whether the detected antibody was a result of inactivated vaccine immunization or live virus infection.	./cache/cord-002624-59nznqsd.txt	./txt/cord-002624-59nznqsd.txt