id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002602-2qvyhjlp	Roy, Amrita	Solution conformations of Zika NS2B-NS3pro and its inhibition by natural products from edible plants	2017-07-10		.txt	text/plain	8987	456	57	Subsequently with selective isotope-labeling using NMR spectroscopy, we demonstrated that C-terminal residues (R73-K100) of NS2B is highly disordered without any stable tertiary and secondary structures in the Zika NS2B-NS3pro complex in the free state. Therefore, our results suggest that in the Zika NS2B-NS3pro complex, NS2B has a portion of residues undergo μs-ms dynamics which made their NMR peaks too broad to be detectable; while the rest of NS2B is highly disordered and lacks tight tertiary packing, which results in a narrowly-dispersed HSQC spectrum (S2B Fig) . Together with recent reports on the crystal structures of Zika NS2B-NS3pro complexes in both open and closed conformations [34, 43] , our current results reveal that in solution the NS2B residues over Arg73-Lys100 are highly disordered in the open conformation. Unfortunately, as previously observed on Dengue-2 NS2B-NS3pro complexes [21, 30, 43] , our linked Zika complex also underwent significant μs-ms dynamics, thus making its NMR signals too broad to be detected (Fig 1A and 1B) .	./cache/cord-002602-2qvyhjlp.txt	./txt/cord-002602-2qvyhjlp.txt