id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002100-dt5zvebj	He, Yonghua	Transgenic Soybean Production of Bioactive Human Epidermal Growth Factor (EGF)	2016-06-17		.txt	text/plain	6795	336	45	Phosphorylation and immunohistochemical assays on the EGF receptor in HeLa cells indicate the EGF protein produced in soybean seed is bioactive and comparable to commercially available human EGF. Epidermal growth factor protein from humans was produced in soybean seeds by constructing a plant gene expression cassette that involved a synthetic codon optimized EGF nucleotide sequence (protein sequence from Genbank accession CCQ43157). To assess the bioactivity of soybean-produced hEGF, samples were prepared from both ShEGF transgenic soybean lines and nontransgenic controls that were used to stimulate HeLa cells to induce EGFR internalization, degradation and phosphorylation. In contrast, samples prepared from control nontransgenic soybeans exhibited no apparent bioactivity showing the degradation and phosphorylation of EGFR is the result of EGF binding of either commercial rhEGF added to the media or from the hEGF produced by the transgenic soybeans.	./cache/cord-002100-dt5zvebj.txt	./txt/cord-002100-dt5zvebj.txt