id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001909-yy9xp5ms	Buß, O.	Statistical Evaluation of HTS Assays for Enzymatic Hydrolysis of β-Keto Esters	2016-01-05		.txt	text/plain	6998	414	49	Two of the tested high throughput screening (HTS) assays depend on coupled enzymatic reactions which detect the alcohol released during ester hydrolysis by luminescence or absorption. The objective of this study is to select an HTS assay to screen for one enzyme for a two-step reaction cascade for the synthesis of β-amino acids. One assay relies on the change of the pH-value, the second is based on enzymatic oxidation of the released ethanol to acetic acid and the third, which we expected to be most sensitive, is based on the oxidation of ethanol to ethanal and hydrogen peroxide which is then converted by horseradish peroxidase (HRP) in a luminescence reaction. According to the other assays a positive control for the evaluation test contained only 2.0 mM ethanol without ester and hydrolase (see also subsection statistical analysis).	./cache/cord-001909-yy9xp5ms.txt	./txt/cord-001909-yy9xp5ms.txt