id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001620-yy5gq0ki	Woo, Hye-Min	Isolation of Single-Stranded DNA Aptamers That Distinguish Influenza Virus Hemagglutinin Subtype H1 from H5	2015-04-22		.txt	text/plain	3871	213	55	These results indicate that the selected aptamers that distinguish H1-HA1 from H5-HA1 can be developed as unique probes for the detection of the H1 subtype of influenza virus. Subtype H5 is known as highly pathogenic in protein (GST-H5-HA1) was incubated with 100 μL of glutathione agarose beads in 100 μL of binding buffer (50 mM Tris/HCl; pH 8.0, 150 mM NaCl, 1.5 mM MgCl 2 , 2 mM DTT, and 1% [w/v] BSA) for 30 min at room temperature with occasional shaking. To select specific ssDNA aptamers that can distinguish H1-HA1 from H5-HA1, counter-SELEX was performed with an ssDNA library of 88-mers containing a randomized sequence region of 45 nucleotides in the center, followed by lambda exonuclease digestion, as shown in Fig 1A. To confirm that the selected aptamers were bound to the sialic acid-binding region of HA1, we performed a flow cytometry analysis (Fig 7) .	./cache/cord-001620-yy5gq0ki.txt	./txt/cord-001620-yy5gq0ki.txt