id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001447-oi7bkm4z	Dhanasekaran, Sakthivel	Toll-Like Receptor Responses to Peste des petits ruminants Virus in Goats and Water Buffalo	2014-11-04		.txt	text/plain	6012	318	51	We examined the replication of PPRV in peripheral blood mononuclear cells (PBMC) of Indian domestic goats and water buffalo and demonstrated that the levels of TLR3 and TLR7 and downstream signalling molecules correlation with susceptibility vs resistance. Upon stimulation of PBMC with synthetic TLR3 and TLR7 agonists or PPRV, the levels of proinflammatory cytokines were found to be significantly higher while immunosuppressive interleukin (IL) 10 levels were lower in PPRV resistant Kanni and Salem Black breeds and water buffalo at transcriptional level, correlating with reduced viralloads in infected PBMC. Infection of peripheral blood mononuclear cells (PBMC) from four breeds of goats and water buffalo resulted in differential viral replication kinetics and inflammatory cytokine profile including IFNa, IFNc and TNFa with differential activation of TLR3 and TLR7. To determine whether the differential IFN and pro-inflammatory cytokine production between Kanni/Salem vs Barbari/ Tellicherry breeds of goats are dependent on single nucleotide polymorphisms (SNPs) in TLR genes, we examined the complete gene sequence of TLR7.	./cache/cord-001447-oi7bkm4z.txt	./txt/cord-001447-oi7bkm4z.txt