id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001142-puj74k7y	Crescenzo-Chaigne, Bernadette	The Panhandle Formed by Influenza A and C Virus NS Non-Coding Regions Determines NS Segment Expression	2013-11-21		.txt	text/plain	3355	183	55	To investigate whether, or not, and how the complete NC regions of a given segment are involved in type specificity, we attempted to rescue, by reverse genetics, type A and C influenza viruses with chimeric non-coding sequences. The sole difference between 5'A/3'C(C5U) and wild-type 5'A/3'A viruses being the 3' distal extremity of the NS segment suggested that the level of NS encoded proteins (i.e. NS1 or/and NS2/NEP) was affected at early stages of infection for this virus (5'A/3'C(C5U)). The major role of the proximal panhandle in type specificity that we identified and the hypothesized involvement of the distal panhandle in transcription need to be tested on the other (2 PFU/cell), viral vRNA and mRNA levels for each segment were evaluated by specific two-step RT-qPCRs previously described [23] .	./cache/cord-001142-puj74k7y.txt	./txt/cord-001142-puj74k7y.txt