id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000947-psguw47w	Feng, Jianyu	A Study of the Mechanism of the Chaperone-like Function of an scFv of Human Creatine Kinase by Computer Simulation	2013-04-24		.txt	text/plain	4685	270	54	We built the model of the single chain antibody (scFv-A4) that increased the stability of human creatine kinase (HCK) by the homology modeling method. Epitopes of human creatine kinase were predicted by computer and then the binding of scFv-A4 and HCK was modeled with computer. To elucidate why scFv-A4 had the chaperone-like function, we combined computer modeling and peptide array membrane technique to study the interactions between scFv-A4 and HCK. Firstly, bioinformatics software tools were used to predict the interacting sites between scFv-A4 and HCK and then the results were combined with the peptide array membrane experiment results to build the 3D models of the binding complex. In complex model-I, scFv-A4 exhibits chaperone-like function by assisting the folding of HCK and stabilizing the whole structure. In this study we combined computer modeling and the peptide array membrane method to investigate the interaction between scFv-A4 and HCK.	./cache/cord-000947-psguw47w.txt	./txt/cord-000947-psguw47w.txt