id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000837-rdpsxb4n	Perez-Pepe, Marcelo	BUHO: A MATLAB Script for the Study of Stress Granules and Processing Bodies by High-Throughput Image Analysis	2012-12-20		.txt	text/plain	7079	366	50	title: BUHO: A MATLAB Script for the Study of Stress Granules and Processing Bodies by High-Throughput Image Analysis Stress granules (SGs) and processing bodies (PBs) belong to a novel family of cellular structures collectively known as mRNA silencing foci that harbour repressed mRNAs and their associated proteins. To assess the suitability of BUHO for the analysis of additional cell structures, we investigated several examples of cytoplasmic foci stained with different strategies, including SGs and PBs in mammalian and insect cells, and synapses and Smaug 1-mRNA silencing foci (S-foci) in primary neurons. Moreover, as expected given that SGs and PBs are morphologically similar, we found that prototype SGs were useful to identify PBs. To test the performance of BUHO in analyzing distances between objects, we focused on the presence of synaptic mRNA silencing foci at the synapse surroundings. With minor modifications, we have successfully applied it for processing images of different magnification and resolution of synapses, SGs, PBs and similar foci in mammalian and Drosophila cell lines as well as in primary cultured neurons.	./cache/cord-000837-rdpsxb4n.txt	./txt/cord-000837-rdpsxb4n.txt