id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000765-r7y1cqou	Chang, Yu-Ming	Functional Studies of ssDNA Binding Ability of MarR Family Protein TcaR from Staphylococcus epidermidis	2012-09-21		.txt	text/plain	5770	317	54	title: Functional Studies of ssDNA Binding Ability of MarR Family Protein TcaR from Staphylococcus epidermidis However, the detailed mechanism of multiple antibiotic resistance regulator (MarR) family proteins such as TcaR is unclear and only restricted on the binding ability of double-strand DNA (dsDNA). Here we show by electrophoretic mobility shift assay (EMSA), electron microscopy (EM), circular dichroism (CD), and Biacore analysis that TcaR can interact strongly with single-stranded DNA (ssDNA), thereby identifying a new role in MarR family proteins. IcaR DNA1 probe duplex of 1 mM was pre-incubated with 2 mM TcaR (dimer) at room temperature for 15 min before mixing with increasing concentration of GC33 ssDNA, followed by the same procedure as described in the legend to Figure 1B . In the EMSA analysis, 1 mM IcaR DNA1 probe duplex was pre-incubated with 1 mM GC33 ssDNA fragment for 15 min at room temperature before mixing with TcaR protein of increasing concentration.	./cache/cord-000765-r7y1cqou.txt	./txt/cord-000765-r7y1cqou.txt