id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000736-6f8vyziv	Pripuzova, Natalia	Development of Real-Time PCR Array for Simultaneous Detection of Eight Human Blood-Borne Viral Pathogens	2012-08-17		.txt	text/plain	6818	328	54	FINDINGS: We developed a real-time PCR array capable of simultaneously detecting eight human viral pathogens: human immunodeficiency virus types 1 and 2 (HIV-1 and -2), hepatitis B virus (HBV), hepatitis C virus (HCV), human T-cell leukemia virus-1 and -2 (HTLV-1 and -2), vaccinia virus (VACV) and West Nile virus (WNV). The analytical sensitivity of each primer set was determined in the single virus testing using FDA/CBER panels (kindly provided by Dr. Stephen Kerby, FDA/CBER) consisting of various amounts of the viruses (0-1,000 genome copies/ml) spiked into the ''normal'' human plasma. The results of sensitivity testing of the real-time PCR array primer sets specific for HIV-1, HIV-2, HBV, HCV, and WNV the with FDA/CBER analytical plasma panels. Tm and C(t) values obtained with primer sets specific for HIV-1, HCV, or HBV in testing of 17 human clinical samples in the format of PCR array targeting eight different viruses.	./cache/cord-000736-6f8vyziv.txt	./txt/cord-000736-6f8vyziv.txt