id author title date pages extension mime words sentences flesch summary cache txt cord-000366-u4649rtx Shan, Tongling Genomic Characterization and High Prevalence of Bocaviruses in Swine 2011-04-15 .txt text/plain 2887 161 55 Using random PCR amplification followed by plasmid subcloning and DNA sequencing, we detected bocavirus related sequences in 9 out of 17 porcine stool samples. Using primer walking, we sequenced the nearly complete genomes of two highly divergent bocaviruses we provisionally named porcine bocavirus 1 isolate H18 (PBoV1-H18) and porcine bocavirus 2 isolate A6 (PBoV2-A6) which differed by 51.8% in their NS1 protein. Presently, the bocavirus genus contains eight members: bovine parvovirus, canine minute virus (CnMV), human bocavirus 1-4 (HBoV1-4), a gorilla bocavirus and a partially sequenced chimpanzee bocavirus [1, 6, 7] . In 2009, a porcine bocalike virus (PBo-likeV) was reported in swine feces with postweaning multisystemic wasting syndrome in Sweden and 1854 bp of its partial genome sequenced [27] . Finally, partial genome sequences of 2.4 Kb from another clade of porcine bocaviruses labeled 6V and 7V where also identified yielding three major bocavirus groups in swine (PBo-likeV, PBoV1/PBoV2, and 6V/7V). ./cache/cord-000366-u4649rtx.txt ./txt/cord-000366-u4649rtx.txt