id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000321-ee7q7jhv	Powell, Michael L.	Further Characterisation of the Translational Termination-Reinitiation Signal of the Influenza B Virus Segment 7 RNA	2011-02-08		.txt	text/plain	9458	433	51	The features identified in caliciviral TURBS suggest a model for termination-reinitiation in which post-termination 40S subunits are tethered to the mRNA through interactions between the mRNA (through motif 1) and 18S rRNA, initiation factors are recruited and the AUG restart codon located, processes which may require precise RNA folding (involving motif 2) within the TURBS. Analysis of primary and secondary structural features of the BM2 signal has revealed that it contains a short TURBS (of 45 nt) which is largely single-stranded, with motif 1 likely to be located in the apical loop of a metastable stem-loop structure when the ribosome is positioned at the termination codon of the upstream ORF [11] . Importantly, mutation of the motif 1 homologue such that it was fully complementary to yeast 18S rRNA led to a significant (p,0.01) increase in BM2fluc synthesis relative to the wild-type BM2 reporter ( Figure 4C ), supporting the view that mRNA:rRNA base pairing is a key determinant in BM2 ORF expression.	./cache/cord-000321-ee7q7jhv.txt	./txt/cord-000321-ee7q7jhv.txt