id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000269-v4jochbe	Wittekindt, Nicola E.	Nodeomics: Pathogen Detection in Vertebrate Lymph Nodes Using Meta-Transcriptomics	2010-10-18		.txt	text/plain	5886	314	44	cDNA libraries from five individuals and two pools of samples were prepared from retropharyngeal lymph node RNA enriched for polyadenylated RNA and sequenced using Roche-454 Life Sciences technology. Representatives of all bacterial phyla were detected in the seven libraries based on protein-coding transcripts indicating that viable microbiota were present in lymph nodes. Based on detection of both rRNA and protein-coding transcripts, we identified two new proteobacterial species; a Helicobacter closely related to Helicobacter cetorum in the Helicobacter pylori/Helicobacter acinonychis complex and an Acinetobacter related to Acinetobacter schindleri. The microbial community of mule deer lymph nodes Detection of protein-coding and ribosomal RNA transcripts provides strong support for the presence of viable and replicating microorganisms. As an alternative approach to identifying bacterial microorganisms present in lymph node tissue, we utilized amplicon DNA library sequencing technology.	./cache/cord-000269-v4jochbe.txt	./txt/cord-000269-v4jochbe.txt