id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000224-2lz03oqb	Porter, Kristen A.	Class II Transactivator (CIITA) Enhances Cytoplasmic Processing of HIV-1 Pr55Gag	2010-06-24		.txt	text/plain	6986	313	44	METHODOLOGY/PRINCIPAL FINDINGS: Here we demonstrate that both stable and transient expression of CIITA in HIV producer cells does not induce HLA-DR-associated intracellular retention of Gag, but does increase the infectivity of virions. We hypothesized that recapitulating endogenous expression of the entire class II antigen presentation pathway in producer cells via expression of CIITA would restore infectious virus release and provide a more physiologically relevant model for HIV-1 assembly studies. Virus release, both infectious and particle titers) were reduced when cells were transfected with either HLA-DR or other components of the MHC class II antigen presentation pathway ( Figure S2 ), confirming a correlation between Gag retention and reduced virus titers in the presence of HLA-DR, as previously demonstrated [8] . Together, these data suggest CIITA has two effects on the HIV replicative cycle in producer cells, both of which are independent of the MHC II antigen processing pathway; i) it does not induce HLA-DR, mediated intracellular retention of Gag and ii) it increases the infectivity of HIV virions.	./cache/cord-000224-2lz03oqb.txt	./txt/cord-000224-2lz03oqb.txt