id	sid	tid	token	lemma	pos
cord-009318-zt1o1bcz	1	1	key	key	NN
cord-009318-zt1o1bcz	1	2	:	:	:
cord-009318-zt1o1bcz	1	3	cord-009318-zt1o1bcz	cord-009318-zt1o1bcz	NNS
cord-009318-zt1o1bcz	1	4	authors	author	NNS
cord-009318-zt1o1bcz	1	5	:	:	:
cord-009318-zt1o1bcz	2	1	Rolando	Rolando	NNP
cord-009318-zt1o1bcz	2	2	,	,	,
cord-009318-zt1o1bcz	2	3	Justin	Justin	NNP
cord-009318-zt1o1bcz	2	4	C	C	NNP
cord-009318-zt1o1bcz	2	5	;	;	:
cord-009318-zt1o1bcz	2	6	Jue	Jue	NNP
cord-009318-zt1o1bcz	2	7	,	,	,
cord-009318-zt1o1bcz	2	8	Erik	Erik	NNP
cord-009318-zt1o1bcz	2	9	;	;	:
cord-009318-zt1o1bcz	2	10	Barlow	Barlow	NNP
cord-009318-zt1o1bcz	2	11	,	,	,
cord-009318-zt1o1bcz	2	12	Jacob	Jacob	NNP
cord-009318-zt1o1bcz	2	13	T	T	NNP
cord-009318-zt1o1bcz	2	14	;	;	:
cord-009318-zt1o1bcz	2	15	Ismagilov	Ismagilov	NNP
cord-009318-zt1o1bcz	2	16	,	,	,
cord-009318-zt1o1bcz	2	17	Rustem	Rustem	NNP
cord-009318-zt1o1bcz	2	18	F	F	NNP
cord-009318-zt1o1bcz	2	19	title	title	NN
cord-009318-zt1o1bcz	2	20	:	:	:
cord-009318-zt1o1bcz	2	21	Real	real	JJ
cord-009318-zt1o1bcz	2	22	-	-	HYPH
cord-009318-zt1o1bcz	2	23	time	time	NN
cord-009318-zt1o1bcz	2	24	kinetics	kinetic	NNS
cord-009318-zt1o1bcz	2	25	and	and	CC
cord-009318-zt1o1bcz	2	26	high	high	JJ
cord-009318-zt1o1bcz	2	27	-	-	HYPH
cord-009318-zt1o1bcz	2	28	resolution	resolution	NN
cord-009318-zt1o1bcz	2	29	melt	melt	NN
cord-009318-zt1o1bcz	2	30	curves	curve	NNS
cord-009318-zt1o1bcz	2	31	in	in	IN
cord-009318-zt1o1bcz	2	32	single	single	JJ
cord-009318-zt1o1bcz	2	33	-	-	HYPH
cord-009318-zt1o1bcz	2	34	molecule	molecule	NN
cord-009318-zt1o1bcz	2	35	digital	digital	JJ
cord-009318-zt1o1bcz	2	36	LAMP	lamp	NN
cord-009318-zt1o1bcz	2	37	to	to	TO
cord-009318-zt1o1bcz	2	38	differentiate	differentiate	VB
cord-009318-zt1o1bcz	2	39	and	and	CC
cord-009318-zt1o1bcz	2	40	study	study	VB
cord-009318-zt1o1bcz	2	41	specific	specific	JJ
cord-009318-zt1o1bcz	2	42	and	and	CC
cord-009318-zt1o1bcz	2	43	non	non	JJ
cord-009318-zt1o1bcz	2	44	-	-	JJ
cord-009318-zt1o1bcz	2	45	specific	specific	JJ
cord-009318-zt1o1bcz	2	46	amplification	amplification	NN
cord-009318-zt1o1bcz	2	47	date	date	NN
cord-009318-zt1o1bcz	2	48	:	:	:
cord-009318-zt1o1bcz	2	49	2020	2020	CD
cord-009318-zt1o1bcz	2	50	-	-	HYPH
cord-009318-zt1o1bcz	2	51	04	04	CD
cord-009318-zt1o1bcz	2	52	-	-	SYM
cord-009318-zt1o1bcz	2	53	17	17	CD
cord-009318-zt1o1bcz	2	54	journal	journal	NN
cord-009318-zt1o1bcz	2	55	:	:	:
cord-009318-zt1o1bcz	2	56	Nucleic	nucleic	JJ
cord-009318-zt1o1bcz	2	57	Acids	acid	NNS
cord-009318-zt1o1bcz	2	58	Res	res	NN
cord-009318-zt1o1bcz	2	59	DOI	doi	NN
cord-009318-zt1o1bcz	2	60	:	:	:
cord-009318-zt1o1bcz	2	61	10.1093	10.1093	CD
cord-009318-zt1o1bcz	2	62	/	/	SYM
cord-009318-zt1o1bcz	2	63	nar	nar	NN
cord-009318-zt1o1bcz	2	64	/	/	SYM
cord-009318-zt1o1bcz	2	65	gkaa099	gkaa099	NNP
cord-009318-zt1o1bcz	2	66	sha	sha	NNP
cord-009318-zt1o1bcz	2	67	:	:	:
cord-009318-zt1o1bcz	2	68	28776e324c2128443197d2c9bc2a9eaa77ed1bf5	28776e324c2128443197d2c9bc2a9eaa77ed1bf5	NNP
cord-009318-zt1o1bcz	2	69	doc_id	doc_id	CD
cord-009318-zt1o1bcz	2	70	:	:	:
cord-009318-zt1o1bcz	2	71	9318	9318	CD
cord-009318-zt1o1bcz	2	72	cord_uid	cord_uid	NNS
cord-009318-zt1o1bcz	2	73	:	:	:
cord-009318-zt1o1bcz	3	1	zt1o1bcz	zt1o1bcz	NNP
cord-009318-zt1o1bcz	3	2	Isothermal	Isothermal	NNP
cord-009318-zt1o1bcz	3	3	amplification	amplification	NN
cord-009318-zt1o1bcz	3	4	assays	assay	NNS
cord-009318-zt1o1bcz	3	5	,	,	,
cord-009318-zt1o1bcz	3	6	such	such	JJ
cord-009318-zt1o1bcz	3	7	as	as	IN
cord-009318-zt1o1bcz	3	8	loop	loop	NN
cord-009318-zt1o1bcz	3	9	-	-	HYPH
cord-009318-zt1o1bcz	3	10	mediated	mediate	VBN
cord-009318-zt1o1bcz	3	11	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	3	12	amplification	amplification	NN
cord-009318-zt1o1bcz	3	13	(	(	-LRB-
cord-009318-zt1o1bcz	3	14	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	3	15	)	)	-RRB-
cord-009318-zt1o1bcz	3	16	,	,	,
cord-009318-zt1o1bcz	3	17	show	show	VBP
cord-009318-zt1o1bcz	3	18	great	great	JJ
cord-009318-zt1o1bcz	3	19	utility	utility	NN
cord-009318-zt1o1bcz	3	20	for	for	IN
cord-009318-zt1o1bcz	3	21	the	the	DT
cord-009318-zt1o1bcz	3	22	development	development	NN
cord-009318-zt1o1bcz	3	23	of	of	IN
cord-009318-zt1o1bcz	3	24	rapid	rapid	JJ
cord-009318-zt1o1bcz	3	25	diagnostics	diagnostic	NNS
cord-009318-zt1o1bcz	3	26	for	for	IN
cord-009318-zt1o1bcz	3	27	infectious	infectious	JJ
cord-009318-zt1o1bcz	3	28	diseases	disease	NNS
cord-009318-zt1o1bcz	3	29	because	because	IN
cord-009318-zt1o1bcz	3	30	they	-PRON-	PRP
cord-009318-zt1o1bcz	3	31	have	have	VBP
cord-009318-zt1o1bcz	3	32	high	high	JJ
cord-009318-zt1o1bcz	3	33	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	3	34	,	,	,
cord-009318-zt1o1bcz	3	35	pathogen	pathogen	NN
cord-009318-zt1o1bcz	3	36	-	-	HYPH
cord-009318-zt1o1bcz	3	37	specificity	specificity	NN
cord-009318-zt1o1bcz	3	38	and	and	CC
cord-009318-zt1o1bcz	3	39	potential	potential	NN
cord-009318-zt1o1bcz	3	40	for	for	IN
cord-009318-zt1o1bcz	3	41	implementation	implementation	NN
cord-009318-zt1o1bcz	3	42	at	at	IN
cord-009318-zt1o1bcz	3	43	the	the	DT
cord-009318-zt1o1bcz	3	44	point	point	NN
cord-009318-zt1o1bcz	3	45	of	of	IN
cord-009318-zt1o1bcz	3	46	care	care	NN
cord-009318-zt1o1bcz	3	47	.	.	.
cord-009318-zt1o1bcz	4	1	However	however	RB
cord-009318-zt1o1bcz	4	2	,	,	,
cord-009318-zt1o1bcz	4	3	elimination	elimination	NN
cord-009318-zt1o1bcz	4	4	of	of	IN
cord-009318-zt1o1bcz	4	5	non	non	JJ
cord-009318-zt1o1bcz	4	6	-	-	JJ
cord-009318-zt1o1bcz	4	7	specific	specific	JJ
cord-009318-zt1o1bcz	4	8	amplification	amplification	NN
cord-009318-zt1o1bcz	4	9	remains	remain	VBZ
cord-009318-zt1o1bcz	4	10	a	a	DT
cord-009318-zt1o1bcz	4	11	key	key	JJ
cord-009318-zt1o1bcz	4	12	challenge	challenge	NN
cord-009318-zt1o1bcz	4	13	for	for	IN
cord-009318-zt1o1bcz	4	14	the	the	DT
cord-009318-zt1o1bcz	4	15	optimization	optimization	NN
cord-009318-zt1o1bcz	4	16	of	of	IN
cord-009318-zt1o1bcz	4	17	LAMP	lamp	NN
cord-009318-zt1o1bcz	4	18	assays	assay	NNS
cord-009318-zt1o1bcz	4	19	.	.	.
cord-009318-zt1o1bcz	5	1	Here	here	RB
cord-009318-zt1o1bcz	5	2	,	,	,
cord-009318-zt1o1bcz	5	3	using	use	VBG
cord-009318-zt1o1bcz	5	4	chlamydia	chlamydia	NNS
cord-009318-zt1o1bcz	5	5	DNA	DNA	NNP
cord-009318-zt1o1bcz	5	6	as	as	IN
cord-009318-zt1o1bcz	5	7	a	a	DT
cord-009318-zt1o1bcz	5	8	clinically	clinically	RB
cord-009318-zt1o1bcz	5	9	relevant	relevant	JJ
cord-009318-zt1o1bcz	5	10	target	target	NN
cord-009318-zt1o1bcz	5	11	and	and	CC
cord-009318-zt1o1bcz	5	12	high	high	JJ
cord-009318-zt1o1bcz	5	13	-	-	HYPH
cord-009318-zt1o1bcz	5	14	throughput	throughput	NN
cord-009318-zt1o1bcz	5	15	sequencing	sequencing	NN
cord-009318-zt1o1bcz	5	16	as	as	IN
cord-009318-zt1o1bcz	5	17	an	an	DT
cord-009318-zt1o1bcz	5	18	analytical	analytical	JJ
cord-009318-zt1o1bcz	5	19	tool	tool	NN
cord-009318-zt1o1bcz	5	20	,	,	,
cord-009318-zt1o1bcz	5	21	we	-PRON-	PRP
cord-009318-zt1o1bcz	5	22	investigate	investigate	VBP
cord-009318-zt1o1bcz	5	23	a	a	DT
cord-009318-zt1o1bcz	5	24	potential	potential	JJ
cord-009318-zt1o1bcz	5	25	mechanism	mechanism	NN
cord-009318-zt1o1bcz	5	26	of	of	IN
cord-009318-zt1o1bcz	5	27	non	non	JJ
cord-009318-zt1o1bcz	5	28	-	-	JJ
cord-009318-zt1o1bcz	5	29	specific	specific	JJ
cord-009318-zt1o1bcz	5	30	amplification	amplification	NN
cord-009318-zt1o1bcz	5	31	.	.	.
cord-009318-zt1o1bcz	6	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	6	2	then	then	RB
cord-009318-zt1o1bcz	6	3	develop	develop	VBP
cord-009318-zt1o1bcz	6	4	a	a	DT
cord-009318-zt1o1bcz	6	5	real	real	JJ
cord-009318-zt1o1bcz	6	6	-	-	HYPH
cord-009318-zt1o1bcz	6	7	time	time	NN
cord-009318-zt1o1bcz	6	8	digital	digital	JJ
cord-009318-zt1o1bcz	6	9	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	6	10	(	(	-LRB-
cord-009318-zt1o1bcz	6	11	dLAMP	dLAMP	NNP
cord-009318-zt1o1bcz	6	12	)	)	-RRB-
cord-009318-zt1o1bcz	6	13	with	with	IN
cord-009318-zt1o1bcz	6	14	high	high	JJ
cord-009318-zt1o1bcz	6	15	-	-	HYPH
cord-009318-zt1o1bcz	6	16	resolution	resolution	NN
cord-009318-zt1o1bcz	6	17	melting	melting	NN
cord-009318-zt1o1bcz	6	18	temperature	temperature	NN
cord-009318-zt1o1bcz	6	19	(	(	-LRB-
cord-009318-zt1o1bcz	6	20	HRM	HRM	NNP
cord-009318-zt1o1bcz	6	21	)	)	-RRB-
cord-009318-zt1o1bcz	6	22	analysis	analysis	NN
cord-009318-zt1o1bcz	6	23	and	and	CC
cord-009318-zt1o1bcz	6	24	use	use	VB
cord-009318-zt1o1bcz	6	25	this	this	DT
cord-009318-zt1o1bcz	6	26	single	single	JJ
cord-009318-zt1o1bcz	6	27	-	-	HYPH
cord-009318-zt1o1bcz	6	28	molecule	molecule	NN
cord-009318-zt1o1bcz	6	29	approach	approach	NN
cord-009318-zt1o1bcz	6	30	to	to	TO
cord-009318-zt1o1bcz	6	31	analyze	analyze	VB
cord-009318-zt1o1bcz	6	32	approximately	approximately	RB
cord-009318-zt1o1bcz	6	33	1.2	1.2	CD
cord-009318-zt1o1bcz	6	34	million	million	CD
cord-009318-zt1o1bcz	6	35	amplification	amplification	NN
cord-009318-zt1o1bcz	6	36	events	event	NNS
cord-009318-zt1o1bcz	6	37	.	.	.
cord-009318-zt1o1bcz	7	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	7	2	show	show	VBP
cord-009318-zt1o1bcz	7	3	that	that	IN
cord-009318-zt1o1bcz	7	4	single	single	JJ
cord-009318-zt1o1bcz	7	5	-	-	HYPH
cord-009318-zt1o1bcz	7	6	molecule	molecule	NN
cord-009318-zt1o1bcz	7	7	HRM	HRM	NNP
cord-009318-zt1o1bcz	7	8	provides	provide	VBZ
cord-009318-zt1o1bcz	7	9	insight	insight	NN
cord-009318-zt1o1bcz	7	10	into	into	IN
cord-009318-zt1o1bcz	7	11	specific	specific	JJ
cord-009318-zt1o1bcz	7	12	and	and	CC
cord-009318-zt1o1bcz	7	13	non	non	JJ
cord-009318-zt1o1bcz	7	14	-	-	JJ
cord-009318-zt1o1bcz	7	15	specific	specific	JJ
cord-009318-zt1o1bcz	7	16	amplification	amplification	NN
cord-009318-zt1o1bcz	7	17	in	in	IN
cord-009318-zt1o1bcz	7	18	LAMP	lamp	NN
cord-009318-zt1o1bcz	7	19	that	that	WDT
cord-009318-zt1o1bcz	7	20	are	be	VBP
cord-009318-zt1o1bcz	7	21	difficult	difficult	JJ
cord-009318-zt1o1bcz	7	22	to	to	TO
cord-009318-zt1o1bcz	7	23	deduce	deduce	VB
cord-009318-zt1o1bcz	7	24	from	from	IN
cord-009318-zt1o1bcz	7	25	bulk	bulk	JJ
cord-009318-zt1o1bcz	7	26	measurements	measurement	NNS
cord-009318-zt1o1bcz	7	27	.	.	.
cord-009318-zt1o1bcz	8	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	8	2	use	use	VBP
cord-009318-zt1o1bcz	8	3	real	real	JJ
cord-009318-zt1o1bcz	8	4	-	-	HYPH
cord-009318-zt1o1bcz	8	5	time	time	NN
cord-009318-zt1o1bcz	8	6	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	8	7	with	with	IN
cord-009318-zt1o1bcz	8	8	HRM	HRM	NNP
cord-009318-zt1o1bcz	8	9	to	to	TO
cord-009318-zt1o1bcz	8	10	evaluate	evaluate	VB
cord-009318-zt1o1bcz	8	11	differences	difference	NNS
cord-009318-zt1o1bcz	8	12	between	between	IN
cord-009318-zt1o1bcz	8	13	polymerase	polymerase	NN
cord-009318-zt1o1bcz	8	14	enzymes	enzyme	NNS
cord-009318-zt1o1bcz	8	15	,	,	,
cord-009318-zt1o1bcz	8	16	the	the	DT
cord-009318-zt1o1bcz	8	17	impact	impact	NN
cord-009318-zt1o1bcz	8	18	of	of	IN
cord-009318-zt1o1bcz	8	19	assay	assay	NN
cord-009318-zt1o1bcz	8	20	parameters	parameter	NNS
cord-009318-zt1o1bcz	8	21	(	(	-LRB-
cord-009318-zt1o1bcz	8	22	e.g.	e.g.	JJ
cord-009318-zt1o1bcz	8	23	time	time	NN
cord-009318-zt1o1bcz	8	24	,	,	,
cord-009318-zt1o1bcz	8	25	rate	rate	NN
cord-009318-zt1o1bcz	8	26	or	or	CC
cord-009318-zt1o1bcz	8	27	florescence	florescence	NN
cord-009318-zt1o1bcz	8	28	intensity	intensity	NN
cord-009318-zt1o1bcz	8	29	)	)	-RRB-
cord-009318-zt1o1bcz	8	30	,	,	,
cord-009318-zt1o1bcz	8	31	and	and	CC
cord-009318-zt1o1bcz	8	32	the	the	DT
cord-009318-zt1o1bcz	8	33	effect	effect	NN
cord-009318-zt1o1bcz	8	34	background	background	NN
cord-009318-zt1o1bcz	8	35	human	human	JJ
cord-009318-zt1o1bcz	8	36	DNA	dna	NN
cord-009318-zt1o1bcz	8	37	.	.	.
cord-009318-zt1o1bcz	9	1	By	by	IN
cord-009318-zt1o1bcz	9	2	differentiating	differentiate	VBG
cord-009318-zt1o1bcz	9	3	true	true	JJ
cord-009318-zt1o1bcz	9	4	and	and	CC
cord-009318-zt1o1bcz	9	5	false	false	JJ
cord-009318-zt1o1bcz	9	6	positives	positive	NNS
cord-009318-zt1o1bcz	9	7	,	,	,
cord-009318-zt1o1bcz	9	8	HRM	HRM	NNP
cord-009318-zt1o1bcz	9	9	enables	enable	VBZ
cord-009318-zt1o1bcz	9	10	determination	determination	NN
cord-009318-zt1o1bcz	9	11	of	of	IN
cord-009318-zt1o1bcz	9	12	the	the	DT
cord-009318-zt1o1bcz	9	13	optimal	optimal	JJ
cord-009318-zt1o1bcz	9	14	assay	assay	NN
cord-009318-zt1o1bcz	9	15	and	and	CC
cord-009318-zt1o1bcz	9	16	analysis	analysis	NN
cord-009318-zt1o1bcz	9	17	parameters	parameter	NNS
cord-009318-zt1o1bcz	9	18	that	that	WDT
cord-009318-zt1o1bcz	9	19	leads	lead	VBZ
cord-009318-zt1o1bcz	9	20	to	to	IN
cord-009318-zt1o1bcz	9	21	the	the	DT
cord-009318-zt1o1bcz	9	22	lowest	low	JJS
cord-009318-zt1o1bcz	9	23	limit	limit	NN
cord-009318-zt1o1bcz	9	24	of	of	IN
cord-009318-zt1o1bcz	9	25	detection	detection	NN
cord-009318-zt1o1bcz	9	26	(	(	-LRB-
cord-009318-zt1o1bcz	9	27	LOD	lod	NN
cord-009318-zt1o1bcz	9	28	)	)	-RRB-
cord-009318-zt1o1bcz	9	29	in	in	IN
cord-009318-zt1o1bcz	9	30	a	a	DT
cord-009318-zt1o1bcz	9	31	digital	digital	JJ
cord-009318-zt1o1bcz	9	32	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	9	33	amplification	amplification	NN
cord-009318-zt1o1bcz	9	34	assay	assay	NN
cord-009318-zt1o1bcz	9	35	.	.	.
cord-009318-zt1o1bcz	10	1	Isothermal	isothermal	JJ
cord-009318-zt1o1bcz	10	2	methods	method	NNS
cord-009318-zt1o1bcz	10	3	,	,	,
cord-009318-zt1o1bcz	10	4	such	such	JJ
cord-009318-zt1o1bcz	10	5	as	as	IN
cord-009318-zt1o1bcz	10	6	loop	loop	NN
cord-009318-zt1o1bcz	10	7	-	-	HYPH
cord-009318-zt1o1bcz	10	8	mediated	mediate	VBN
cord-009318-zt1o1bcz	10	9	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	10	10	amplification	amplification	NN
cord-009318-zt1o1bcz	10	11	(	(	-LRB-
cord-009318-zt1o1bcz	10	12	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	10	13	)	)	-RRB-
cord-009318-zt1o1bcz	10	14	,	,	,
cord-009318-zt1o1bcz	10	15	are	be	VBP
cord-009318-zt1o1bcz	10	16	attractive	attractive	JJ
cord-009318-zt1o1bcz	10	17	for	for	IN
cord-009318-zt1o1bcz	10	18	nucleic	nucleic	JJ
cord-009318-zt1o1bcz	10	19	acid	acid	NN
cord-009318-zt1o1bcz	10	20	amplification	amplification	NN
cord-009318-zt1o1bcz	10	21	tests	test	NNS
cord-009318-zt1o1bcz	10	22	(	(	-LRB-
cord-009318-zt1o1bcz	10	23	NAATs	NAATs	NNP
cord-009318-zt1o1bcz	10	24	)	)	-RRB-
cord-009318-zt1o1bcz	10	25	in	in	IN
cord-009318-zt1o1bcz	10	26	point	point	NN
cord-009318-zt1o1bcz	10	27	-	-	HYPH
cord-009318-zt1o1bcz	10	28	of	of	IN
cord-009318-zt1o1bcz	10	29	-	-	HYPH
cord-009318-zt1o1bcz	10	30	care	care	NN
cord-009318-zt1o1bcz	10	31	and	and	CC
cord-009318-zt1o1bcz	10	32	limited	limited	JJ
cord-009318-zt1o1bcz	10	33	-	-	HYPH
cord-009318-zt1o1bcz	10	34	resource	resource	NN
cord-009318-zt1o1bcz	10	35	settings	setting	NNS
cord-009318-zt1o1bcz	10	36	(	(	-LRB-
cord-009318-zt1o1bcz	10	37	1	1	CD
cord-009318-zt1o1bcz	10	38	,	,	,
cord-009318-zt1o1bcz	10	39	2	2	CD
cord-009318-zt1o1bcz	10	40	)	)	-RRB-
cord-009318-zt1o1bcz	10	41	.	.	.
cord-009318-zt1o1bcz	11	1	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	11	2	in	in	IN
cord-009318-zt1o1bcz	11	3	particular	particular	JJ
cord-009318-zt1o1bcz	11	4	shows	show	NNS
cord-009318-zt1o1bcz	11	5	promise	promise	NN
cord-009318-zt1o1bcz	11	6	as	as	IN
cord-009318-zt1o1bcz	11	7	a	a	DT
cord-009318-zt1o1bcz	11	8	NAAT	NAAT	NNP
cord-009318-zt1o1bcz	11	9	with	with	IN
cord-009318-zt1o1bcz	11	10	fewer	few	JJR
cord-009318-zt1o1bcz	11	11	hardware	hardware	NN
cord-009318-zt1o1bcz	11	12	requirements	requirement	NNS
cord-009318-zt1o1bcz	11	13	compared	compare	VBN
cord-009318-zt1o1bcz	11	14	with	with	IN
cord-009318-zt1o1bcz	11	15	polymerase	polymerase	NN
cord-009318-zt1o1bcz	11	16	chain	chain	NN
cord-009318-zt1o1bcz	11	17	reaction	reaction	NN
cord-009318-zt1o1bcz	11	18	(	(	-LRB-
cord-009318-zt1o1bcz	11	19	PCR	PCR	NNP
cord-009318-zt1o1bcz	11	20	)	)	-RRB-
cord-009318-zt1o1bcz	11	21	(	(	-LRB-
cord-009318-zt1o1bcz	11	22	3	3	CD
cord-009318-zt1o1bcz	11	23	)	)	-RRB-
cord-009318-zt1o1bcz	11	24	.	.	.
cord-009318-zt1o1bcz	12	1	Despite	despite	IN
cord-009318-zt1o1bcz	12	2	advancements	advancement	NNS
cord-009318-zt1o1bcz	12	3	,	,	,
cord-009318-zt1o1bcz	12	4	the	the	DT
cord-009318-zt1o1bcz	12	5	ability	ability	NN
cord-009318-zt1o1bcz	12	6	to	to	TO
cord-009318-zt1o1bcz	12	7	optimize	optimize	VB
cord-009318-zt1o1bcz	12	8	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	12	9	NAATs	NAATs	NNP
cord-009318-zt1o1bcz	12	10	for	for	IN
cord-009318-zt1o1bcz	12	11	a	a	DT
cord-009318-zt1o1bcz	12	12	specific	specific	JJ
cord-009318-zt1o1bcz	12	13	target	target	NN
cord-009318-zt1o1bcz	12	14	sequence	sequence	NN
cord-009318-zt1o1bcz	12	15	and	and	CC
cord-009318-zt1o1bcz	12	16	primer	primer	NN
cord-009318-zt1o1bcz	12	17	set	set	VBN
cord-009318-zt1o1bcz	12	18	(	(	-LRB-
cord-009318-zt1o1bcz	12	19	specific	specific	JJ
cord-009318-zt1o1bcz	12	20	to	to	IN
cord-009318-zt1o1bcz	12	21	a	a	DT
cord-009318-zt1o1bcz	12	22	target	target	NN
cord-009318-zt1o1bcz	12	23	organism	organism	NN
cord-009318-zt1o1bcz	12	24	)	)	-RRB-
cord-009318-zt1o1bcz	12	25	remains	remain	VBZ
cord-009318-zt1o1bcz	12	26	constrained	constrain	VBN
cord-009318-zt1o1bcz	12	27	by	by	IN
cord-009318-zt1o1bcz	12	28	a	a	DT
cord-009318-zt1o1bcz	12	29	limited	limited	JJ
cord-009318-zt1o1bcz	12	30	understanding	understanding	NN
cord-009318-zt1o1bcz	12	31	of	of	IN
cord-009318-zt1o1bcz	12	32	how	how	WRB
cord-009318-zt1o1bcz	12	33	amplification	amplification	NN
cord-009318-zt1o1bcz	12	34	is	be	VBZ
cord-009318-zt1o1bcz	12	35	affected	affect	VBN
cord-009318-zt1o1bcz	12	36	by	by	IN
cord-009318-zt1o1bcz	12	37	myriad	myriad	JJ
cord-009318-zt1o1bcz	12	38	factors	factor	NNS
cord-009318-zt1o1bcz	12	39	,	,	,
cord-009318-zt1o1bcz	12	40	including	include	VBG
cord-009318-zt1o1bcz	12	41	polymerase	polymerase	NN
cord-009318-zt1o1bcz	12	42	choice	choice	NN
cord-009318-zt1o1bcz	12	43	,	,	,
cord-009318-zt1o1bcz	12	44	primer	primer	NN
cord-009318-zt1o1bcz	12	45	design	design	NN
cord-009318-zt1o1bcz	12	46	,	,	,
cord-009318-zt1o1bcz	12	47	temperature	temperature	NN
cord-009318-zt1o1bcz	12	48	,	,	,
cord-009318-zt1o1bcz	12	49	time	time	NN
cord-009318-zt1o1bcz	12	50	and	and	CC
cord-009318-zt1o1bcz	12	51	ion	ion	NN
cord-009318-zt1o1bcz	12	52	concentrations	concentration	NNS
cord-009318-zt1o1bcz	12	53	.	.	.
cord-009318-zt1o1bcz	13	1	In	in	IN
cord-009318-zt1o1bcz	13	2	particular	particular	JJ
cord-009318-zt1o1bcz	13	3	,	,	,
cord-009318-zt1o1bcz	13	4	addressing	address	VBG
cord-009318-zt1o1bcz	13	5	non	non	JJ
cord-009318-zt1o1bcz	13	6	-	-	JJ
cord-009318-zt1o1bcz	13	7	specific	specific	JJ
cord-009318-zt1o1bcz	13	8	amplification	amplification	NN
cord-009318-zt1o1bcz	13	9	remains	remain	VBZ
cord-009318-zt1o1bcz	13	10	a	a	DT
cord-009318-zt1o1bcz	13	11	core	core	NN
cord-009318-zt1o1bcz	13	12	problem	problem	NN
cord-009318-zt1o1bcz	13	13	as	as	IN
cord-009318-zt1o1bcz	13	14	it	-PRON-	PRP
cord-009318-zt1o1bcz	13	15	constrains	constrain	VBZ
cord-009318-zt1o1bcz	13	16	an	an	DT
cord-009318-zt1o1bcz	13	17	assay	assay	NN
cord-009318-zt1o1bcz	13	18	's	's	POS
cord-009318-zt1o1bcz	13	19	limit	limit	NN
cord-009318-zt1o1bcz	13	20	of	of	IN
cord-009318-zt1o1bcz	13	21	detection	detection	NN
cord-009318-zt1o1bcz	13	22	(	(	-LRB-
cord-009318-zt1o1bcz	13	23	LOD	lod	NN
cord-009318-zt1o1bcz	13	24	)	)	-RRB-
cord-009318-zt1o1bcz	13	25	.	.	.
cord-009318-zt1o1bcz	14	1	In	in	IN
cord-009318-zt1o1bcz	14	2	reactions	reaction	NNS
cord-009318-zt1o1bcz	14	3	containing	contain	VBG
cord-009318-zt1o1bcz	14	4	template	template	NN
cord-009318-zt1o1bcz	14	5	target	target	NN
cord-009318-zt1o1bcz	14	6	molecules	molecule	NNS
cord-009318-zt1o1bcz	14	7	,	,	,
cord-009318-zt1o1bcz	14	8	both	both	DT
cord-009318-zt1o1bcz	14	9	specific	specific	JJ
cord-009318-zt1o1bcz	14	10	and	and	CC
cord-009318-zt1o1bcz	14	11	non	non	JJ
cord-009318-zt1o1bcz	14	12	-	-	JJ
cord-009318-zt1o1bcz	14	13	specific	specific	JJ
cord-009318-zt1o1bcz	14	14	amplification	amplification	NN
cord-009318-zt1o1bcz	14	15	reactions	reaction	NNS
cord-009318-zt1o1bcz	14	16	may	may	MD
cord-009318-zt1o1bcz	14	17	occur	occur	VB
cord-009318-zt1o1bcz	14	18	.	.	.
cord-009318-zt1o1bcz	15	1	Unlike	unlike	IN
cord-009318-zt1o1bcz	15	2	PCR	PCR	NNP
cord-009318-zt1o1bcz	15	3	,	,	,
cord-009318-zt1o1bcz	15	4	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	15	5	lacks	lack	VBZ
cord-009318-zt1o1bcz	15	6	a	a	DT
cord-009318-zt1o1bcz	15	7	temperature	temperature	NN
cord-009318-zt1o1bcz	15	8	-	-	HYPH
cord-009318-zt1o1bcz	15	9	gating	gate	VBG
cord-009318-zt1o1bcz	15	10	mechanism	mechanism	NN
cord-009318-zt1o1bcz	15	11	,	,	,
cord-009318-zt1o1bcz	15	12	so	so	CC
cord-009318-zt1o1bcz	15	13	non	non	JJ
cord-009318-zt1o1bcz	15	14	-	-	JJ
cord-009318-zt1o1bcz	15	15	specific	specific	JJ
cord-009318-zt1o1bcz	15	16	reactions	reaction	NNS
cord-009318-zt1o1bcz	15	17	consume	consume	VBP
cord-009318-zt1o1bcz	15	18	reagents	reagent	NNS
cord-009318-zt1o1bcz	15	19	and	and	CC
cord-009318-zt1o1bcz	15	20	compete	compete	VB
cord-009318-zt1o1bcz	15	21	with	with	IN
cord-009318-zt1o1bcz	15	22	specific	specific	JJ
cord-009318-zt1o1bcz	15	23	amplification	amplification	NN
cord-009318-zt1o1bcz	15	24	impacting	impact	VBG
cord-009318-zt1o1bcz	15	25	its	-PRON-	PRP$
cord-009318-zt1o1bcz	15	26	kinetics	kinetic	NNS
cord-009318-zt1o1bcz	15	27	.	.	.
cord-009318-zt1o1bcz	16	1	The	the	DT
cord-009318-zt1o1bcz	16	2	presence	presence	NN
cord-009318-zt1o1bcz	16	3	of	of	IN
cord-009318-zt1o1bcz	16	4	non	non	JJ
cord-009318-zt1o1bcz	16	5	-	-	JJ
cord-009318-zt1o1bcz	16	6	specific	specific	JJ
cord-009318-zt1o1bcz	16	7	amplicons	amplicon	NNS
cord-009318-zt1o1bcz	16	8	therefore	therefore	RB
cord-009318-zt1o1bcz	16	9	adversely	adversely	RB
cord-009318-zt1o1bcz	16	10	impacts	impact	NNS
cord-009318-zt1o1bcz	16	11	both	both	CC
cord-009318-zt1o1bcz	16	12	the	the	DT
cord-009318-zt1o1bcz	16	13	assay	assay	NN
cord-009318-zt1o1bcz	16	14	's	's	POS
cord-009318-zt1o1bcz	16	15	analytical	analytical	JJ
cord-009318-zt1o1bcz	16	16	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	16	17	(	(	-LRB-
cord-009318-zt1o1bcz	16	18	the	the	DT
cord-009318-zt1o1bcz	16	19	fewest	few	JJS
cord-009318-zt1o1bcz	16	20	template	template	NN
cord-009318-zt1o1bcz	16	21	molecules	molecule	NNS
cord-009318-zt1o1bcz	16	22	that	that	WDT
cord-009318-zt1o1bcz	16	23	can	can	MD
cord-009318-zt1o1bcz	16	24	be	be	VB
cord-009318-zt1o1bcz	16	25	detected	detect	VBN
cord-009318-zt1o1bcz	16	26	)	)	-RRB-
cord-009318-zt1o1bcz	16	27	and	and	CC
cord-009318-zt1o1bcz	16	28	its	-PRON-	PRP$
cord-009318-zt1o1bcz	16	29	analytical	analytical	JJ
cord-009318-zt1o1bcz	16	30	specificity	specificity	NN
cord-009318-zt1o1bcz	16	31	(	(	-LRB-
cord-009318-zt1o1bcz	16	32	ability	ability	NN
cord-009318-zt1o1bcz	16	33	to	to	TO
cord-009318-zt1o1bcz	16	34	detect	detect	VB
cord-009318-zt1o1bcz	16	35	the	the	DT
cord-009318-zt1o1bcz	16	36	target	target	NN
cord-009318-zt1o1bcz	16	37	template	template	NN
cord-009318-zt1o1bcz	16	38	in	in	IN
cord-009318-zt1o1bcz	16	39	the	the	DT
cord-009318-zt1o1bcz	16	40	presence	presence	NN
cord-009318-zt1o1bcz	16	41	of	of	IN
cord-009318-zt1o1bcz	16	42	competing	compete	VBG
cord-009318-zt1o1bcz	16	43	reactions	reaction	NNS
cord-009318-zt1o1bcz	16	44	)	)	-RRB-
cord-009318-zt1o1bcz	16	45	.	.	.
cord-009318-zt1o1bcz	17	1	Classifying	classify	VBG
cord-009318-zt1o1bcz	17	2	reactions	reaction	NNS
cord-009318-zt1o1bcz	17	3	as	as	IN
cord-009318-zt1o1bcz	17	4	either	either	DT
cord-009318-zt1o1bcz	17	5	specific	specific	JJ
cord-009318-zt1o1bcz	17	6	or	or	CC
cord-009318-zt1o1bcz	17	7	non	non	JJ
cord-009318-zt1o1bcz	17	8	-	-	JJ
cord-009318-zt1o1bcz	17	9	specific	specific	JJ
cord-009318-zt1o1bcz	17	10	amplification	amplification	NN
cord-009318-zt1o1bcz	17	11	would	would	MD
cord-009318-zt1o1bcz	17	12	therefore	therefore	RB
cord-009318-zt1o1bcz	17	13	be	be	VB
cord-009318-zt1o1bcz	17	14	invaluable	invaluable	JJ
cord-009318-zt1o1bcz	17	15	both	both	CC
cord-009318-zt1o1bcz	17	16	during	during	IN
cord-009318-zt1o1bcz	17	17	assay	assay	NN
cord-009318-zt1o1bcz	17	18	optimization	optimization	NN
cord-009318-zt1o1bcz	17	19	and	and	CC
cord-009318-zt1o1bcz	17	20	assay	assay	NN
cord-009318-zt1o1bcz	17	21	deployment	deployment	NN
cord-009318-zt1o1bcz	17	22	in	in	IN
cord-009318-zt1o1bcz	17	23	clinical	clinical	JJ
cord-009318-zt1o1bcz	17	24	diagnostics	diagnostic	NNS
cord-009318-zt1o1bcz	17	25	.	.	.
cord-009318-zt1o1bcz	18	1	Substantial	substantial	JJ
cord-009318-zt1o1bcz	18	2	research	research	NN
cord-009318-zt1o1bcz	18	3	is	be	VBZ
cord-009318-zt1o1bcz	18	4	focused	focus	VBN
cord-009318-zt1o1bcz	18	5	on	on	IN
cord-009318-zt1o1bcz	18	6	using	use	VBG
cord-009318-zt1o1bcz	18	7	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	18	8	amplification	amplification	NN
cord-009318-zt1o1bcz	18	9	chemistries	chemistry	NNS
cord-009318-zt1o1bcz	18	10	for	for	IN
cord-009318-zt1o1bcz	18	11	diagnosis	diagnosis	NN
cord-009318-zt1o1bcz	18	12	of	of	IN
cord-009318-zt1o1bcz	18	13	infectious	infectious	JJ
cord-009318-zt1o1bcz	18	14	disease	disease	NN
cord-009318-zt1o1bcz	18	15	.	.	.
cord-009318-zt1o1bcz	19	1	For	for	IN
cord-009318-zt1o1bcz	19	2	example	example	NN
cord-009318-zt1o1bcz	19	3	,	,	,
cord-009318-zt1o1bcz	19	4	chlamydia	chlamydia	NNP
cord-009318-zt1o1bcz	19	5	(	(	-LRB-
cord-009318-zt1o1bcz	19	6	caused	cause	VBN
cord-009318-zt1o1bcz	19	7	by	by	IN
cord-009318-zt1o1bcz	19	8	the	the	DT
cord-009318-zt1o1bcz	19	9	pathogen	pathogen	NN
cord-009318-zt1o1bcz	19	10	Chlamydia	Chlamydia	NNP
cord-009318-zt1o1bcz	19	11	trachomatis	trachomatis	NN
cord-009318-zt1o1bcz	19	12	,	,	,
cord-009318-zt1o1bcz	19	13	CT	CT	NNP
cord-009318-zt1o1bcz	19	14	)	)	-RRB-
cord-009318-zt1o1bcz	19	15	is	be	VBZ
cord-009318-zt1o1bcz	19	16	the	the	DT
cord-009318-zt1o1bcz	19	17	most	most	RBS
cord-009318-zt1o1bcz	19	18	common	common	JJ
cord-009318-zt1o1bcz	19	19	sexually	sexually	RB
cord-009318-zt1o1bcz	19	20	transmitted	transmit	VBN
cord-009318-zt1o1bcz	19	21	infection	infection	NN
cord-009318-zt1o1bcz	19	22	worldwide	worldwide	RB
cord-009318-zt1o1bcz	19	23	,	,	,
cord-009318-zt1o1bcz	19	24	with	with	IN
cord-009318-zt1o1bcz	19	25	more	more	JJR
cord-009318-zt1o1bcz	19	26	than	than	IN
cord-009318-zt1o1bcz	19	27	110	110	CD
cord-009318-zt1o1bcz	19	28	million	million	CD
cord-009318-zt1o1bcz	19	29	cases	case	NNS
cord-009318-zt1o1bcz	19	30	reported	report	VBN
cord-009318-zt1o1bcz	19	31	annually	annually	RB
cord-009318-zt1o1bcz	19	32	(	(	-LRB-
cord-009318-zt1o1bcz	19	33	4	4	CD
cord-009318-zt1o1bcz	19	34	)	)	-RRB-
cord-009318-zt1o1bcz	19	35	.	.	.
cord-009318-zt1o1bcz	20	1	Diagnosis	diagnosis	NN
cord-009318-zt1o1bcz	20	2	of	of	IN
cord-009318-zt1o1bcz	20	3	CT	CT	NNP
cord-009318-zt1o1bcz	20	4	infections	infection	NNS
cord-009318-zt1o1bcz	20	5	is	be	VBZ
cord-009318-zt1o1bcz	20	6	challenged	challenge	VBN
cord-009318-zt1o1bcz	20	7	by	by	IN
cord-009318-zt1o1bcz	20	8	a	a	DT
cord-009318-zt1o1bcz	20	9	lack	lack	NN
cord-009318-zt1o1bcz	20	10	of	of	IN
cord-009318-zt1o1bcz	20	11	standard	standard	JJ
cord-009318-zt1o1bcz	20	12	symptoms	symptom	NNS
cord-009318-zt1o1bcz	20	13	(	(	-LRB-
cord-009318-zt1o1bcz	20	14	many	many	JJ
cord-009318-zt1o1bcz	20	15	infections	infection	NNS
cord-009318-zt1o1bcz	20	16	are	be	VBP
cord-009318-zt1o1bcz	20	17	asymptomatic	asymptomatic	JJ
cord-009318-zt1o1bcz	20	18	)	)	-RRB-
cord-009318-zt1o1bcz	21	1	(	(	-LRB-
cord-009318-zt1o1bcz	21	2	5	5	LS
cord-009318-zt1o1bcz	21	3	)	)	-RRB-
cord-009318-zt1o1bcz	21	4	and	and	CC
cord-009318-zt1o1bcz	21	5	the	the	DT
cord-009318-zt1o1bcz	21	6	presence	presence	NN
cord-009318-zt1o1bcz	21	7	of	of	IN
cord-009318-zt1o1bcz	21	8	mixed	mix	VBN
cord-009318-zt1o1bcz	21	9	flora	flora	NNS
cord-009318-zt1o1bcz	21	10	(	(	-LRB-
cord-009318-zt1o1bcz	21	11	particularly	particularly	RB
cord-009318-zt1o1bcz	21	12	in	in	IN
cord-009318-zt1o1bcz	21	13	the	the	DT
cord-009318-zt1o1bcz	21	14	female	female	JJ
cord-009318-zt1o1bcz	21	15	reproductive	reproductive	JJ
cord-009318-zt1o1bcz	21	16	tract	tract	NN
cord-009318-zt1o1bcz	21	17	)	)	-RRB-
cord-009318-zt1o1bcz	21	18	(	(	-LRB-
cord-009318-zt1o1bcz	21	19	6	6	CD
cord-009318-zt1o1bcz	21	20	)	)	-RRB-
cord-009318-zt1o1bcz	21	21	.	.	.
cord-009318-zt1o1bcz	22	1	Thus	thus	RB
cord-009318-zt1o1bcz	22	2	,	,	,
cord-009318-zt1o1bcz	22	3	rapid	rapid	JJ
cord-009318-zt1o1bcz	22	4	NAATs	naat	NNS
cord-009318-zt1o1bcz	22	5	with	with	IN
cord-009318-zt1o1bcz	22	6	high	high	JJ
cord-009318-zt1o1bcz	22	7	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	22	8	and	and	CC
cord-009318-zt1o1bcz	22	9	specificity	specificity	NN
cord-009318-zt1o1bcz	22	10	are	be	VBP
cord-009318-zt1o1bcz	22	11	critically	critically	RB
cord-009318-zt1o1bcz	22	12	needed	need	VBN
cord-009318-zt1o1bcz	22	13	,	,	,
cord-009318-zt1o1bcz	22	14	especially	especially	RB
cord-009318-zt1o1bcz	22	15	NAATs	NAATs	NNP
cord-009318-zt1o1bcz	22	16	that	that	WDT
cord-009318-zt1o1bcz	22	17	can	can	MD
cord-009318-zt1o1bcz	22	18	deal	deal	VB
cord-009318-zt1o1bcz	22	19	with	with	IN
cord-009318-zt1o1bcz	22	20	the	the	DT
cord-009318-zt1o1bcz	22	21	high	high	JJ
cord-009318-zt1o1bcz	22	22	levels	level	NNS
cord-009318-zt1o1bcz	22	23	of	of	IN
cord-009318-zt1o1bcz	22	24	host	host	NN
cord-009318-zt1o1bcz	22	25	or	or	CC
cord-009318-zt1o1bcz	22	26	background	background	NN
cord-009318-zt1o1bcz	22	27	DNA	dna	NN
cord-009318-zt1o1bcz	22	28	likely	likely	JJ
cord-009318-zt1o1bcz	22	29	to	to	TO
cord-009318-zt1o1bcz	22	30	be	be	VB
cord-009318-zt1o1bcz	22	31	present	present	JJ
cord-009318-zt1o1bcz	22	32	in	in	IN
cord-009318-zt1o1bcz	22	33	clinical	clinical	JJ
cord-009318-zt1o1bcz	22	34	samples	sample	NNS
cord-009318-zt1o1bcz	22	35	such	such	JJ
cord-009318-zt1o1bcz	22	36	as	as	IN
cord-009318-zt1o1bcz	22	37	urine	urine	NN
cord-009318-zt1o1bcz	22	38	samples	sample	NNS
cord-009318-zt1o1bcz	22	39	and	and	CC
cord-009318-zt1o1bcz	22	40	swabs	swab	NNS
cord-009318-zt1o1bcz	22	41	(	(	-LRB-
cord-009318-zt1o1bcz	22	42	7	7	CD
cord-009318-zt1o1bcz	22	43	,	,	,
cord-009318-zt1o1bcz	22	44	8)	8)	NN
cord-009318-zt1o1bcz	22	45	.	.	.
cord-009318-zt1o1bcz	23	1	Optimizing	optimize	VBG
cord-009318-zt1o1bcz	23	2	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	23	3	for	for	IN
cord-009318-zt1o1bcz	23	4	CT	CT	NNP
cord-009318-zt1o1bcz	23	5	and	and	CC
cord-009318-zt1o1bcz	23	6	other	other	JJ
cord-009318-zt1o1bcz	23	7	infectious	infectious	JJ
cord-009318-zt1o1bcz	23	8	pathogens	pathogen	NNS
cord-009318-zt1o1bcz	23	9	requires	require	VBZ
cord-009318-zt1o1bcz	23	10	addressing	address	VBG
cord-009318-zt1o1bcz	23	11	and	and	CC
cord-009318-zt1o1bcz	23	12	reducing	reduce	VBG
cord-009318-zt1o1bcz	23	13	non	non	JJ
cord-009318-zt1o1bcz	23	14	-	-	JJ
cord-009318-zt1o1bcz	23	15	specific	specific	JJ
cord-009318-zt1o1bcz	23	16	amplification	amplification	NN
cord-009318-zt1o1bcz	23	17	or	or	CC
cord-009318-zt1o1bcz	23	18	a	a	DT
cord-009318-zt1o1bcz	23	19	method	method	NN
cord-009318-zt1o1bcz	23	20	for	for	IN
cord-009318-zt1o1bcz	23	21	separating	separate	VBG
cord-009318-zt1o1bcz	23	22	non	non	JJ
cord-009318-zt1o1bcz	23	23	-	-	JJ
cord-009318-zt1o1bcz	23	24	specific	specific	JJ
cord-009318-zt1o1bcz	23	25	reactions	reaction	NNS
cord-009318-zt1o1bcz	23	26	from	from	IN
cord-009318-zt1o1bcz	23	27	specific	specific	JJ
cord-009318-zt1o1bcz	23	28	amplification	amplification	NN
cord-009318-zt1o1bcz	23	29	.	.	.
cord-009318-zt1o1bcz	24	1	Reactions	reaction	NNS
cord-009318-zt1o1bcz	24	2	run	run	VBP
cord-009318-zt1o1bcz	24	3	in	in	IN
cord-009318-zt1o1bcz	24	4	bulk	bulk	NN
cord-009318-zt1o1bcz	24	5	(	(	-LRB-
cord-009318-zt1o1bcz	24	6	i.e.	i.e.	FW
cord-009318-zt1o1bcz	24	7	in	in	IN
cord-009318-zt1o1bcz	24	8	a	a	DT
cord-009318-zt1o1bcz	24	9	tube	tube	NN
cord-009318-zt1o1bcz	24	10	)	)	-RRB-
cord-009318-zt1o1bcz	24	11	in	in	IN
cord-009318-zt1o1bcz	24	12	the	the	DT
cord-009318-zt1o1bcz	24	13	absence	absence	NN
cord-009318-zt1o1bcz	24	14	of	of	IN
cord-009318-zt1o1bcz	24	15	template	template	NN
cord-009318-zt1o1bcz	24	16	can	can	MD
cord-009318-zt1o1bcz	24	17	be	be	VB
cord-009318-zt1o1bcz	24	18	informative	informative	JJ
cord-009318-zt1o1bcz	24	19	to	to	TO
cord-009318-zt1o1bcz	24	20	provide	provide	VB
cord-009318-zt1o1bcz	24	21	information	information	NN
cord-009318-zt1o1bcz	24	22	on	on	IN
cord-009318-zt1o1bcz	24	23	performance	performance	NN
cord-009318-zt1o1bcz	24	24	of	of	IN
cord-009318-zt1o1bcz	24	25	non	non	JJ
cord-009318-zt1o1bcz	24	26	-	-	JJ
cord-009318-zt1o1bcz	24	27	specific	specific	JJ
cord-009318-zt1o1bcz	24	28	amplification	amplification	NN
cord-009318-zt1o1bcz	24	29	.	.	.
cord-009318-zt1o1bcz	25	1	Another	another	DT
cord-009318-zt1o1bcz	25	2	method	method	NN
cord-009318-zt1o1bcz	25	3	to	to	TO
cord-009318-zt1o1bcz	25	4	identify	identify	VB
cord-009318-zt1o1bcz	25	5	non	non	JJ
cord-009318-zt1o1bcz	25	6	-	-	JJ
cord-009318-zt1o1bcz	25	7	specific	specific	JJ
cord-009318-zt1o1bcz	25	8	amplification	amplification	NN
cord-009318-zt1o1bcz	25	9	includes	include	VBZ
cord-009318-zt1o1bcz	25	10	mathematical	mathematical	JJ
cord-009318-zt1o1bcz	25	11	modeling	modeling	NN
cord-009318-zt1o1bcz	25	12	in	in	IN
cord-009318-zt1o1bcz	25	13	conjunction	conjunction	NN
cord-009318-zt1o1bcz	25	14	with	with	IN
cord-009318-zt1o1bcz	25	15	electrophoresis	electrophoresis	NN
cord-009318-zt1o1bcz	25	16	to	to	TO
cord-009318-zt1o1bcz	25	17	distinguish	distinguish	VB
cord-009318-zt1o1bcz	25	18	between	between	IN
cord-009318-zt1o1bcz	25	19	non	non	JJ
cord-009318-zt1o1bcz	25	20	-	-	JJ
cord-009318-zt1o1bcz	25	21	specific	specific	JJ
cord-009318-zt1o1bcz	25	22	and	and	CC
cord-009318-zt1o1bcz	25	23	specific	specific	JJ
cord-009318-zt1o1bcz	25	24	banding	banding	NN
cord-009318-zt1o1bcz	25	25	patterns	pattern	NNS
cord-009318-zt1o1bcz	25	26	(	(	-LRB-
cord-009318-zt1o1bcz	25	27	9	9	CD
cord-009318-zt1o1bcz	25	28	)	)	-RRB-
cord-009318-zt1o1bcz	25	29	.	.	.
cord-009318-zt1o1bcz	26	1	However	however	RB
cord-009318-zt1o1bcz	26	2	,	,	,
cord-009318-zt1o1bcz	26	3	in	in	IN
cord-009318-zt1o1bcz	26	4	the	the	DT
cord-009318-zt1o1bcz	26	5	presence	presence	NN
cord-009318-zt1o1bcz	26	6	of	of	IN
cord-009318-zt1o1bcz	26	7	template	template	NN
cord-009318-zt1o1bcz	26	8	,	,	,
cord-009318-zt1o1bcz	26	9	although	although	IN
cord-009318-zt1o1bcz	26	10	specific	specific	JJ
cord-009318-zt1o1bcz	26	11	and	and	CC
cord-009318-zt1o1bcz	26	12	non	non	JJ
cord-009318-zt1o1bcz	26	13	-	-	JJ
cord-009318-zt1o1bcz	26	14	specific	specific	JJ
cord-009318-zt1o1bcz	26	15	reactions	reaction	NNS
cord-009318-zt1o1bcz	26	16	occur	occur	VBP
cord-009318-zt1o1bcz	26	17	simultaneously	simultaneously	RB
cord-009318-zt1o1bcz	26	18	,	,	,
cord-009318-zt1o1bcz	26	19	they	-PRON-	PRP
cord-009318-zt1o1bcz	26	20	can	can	MD
cord-009318-zt1o1bcz	26	21	not	not	RB
cord-009318-zt1o1bcz	26	22	be	be	VB
cord-009318-zt1o1bcz	26	23	monitored	monitor	VBN
cord-009318-zt1o1bcz	26	24	simultaneously	simultaneously	RB
cord-009318-zt1o1bcz	26	25	.	.	.
cord-009318-zt1o1bcz	27	1	Thus	thus	RB
cord-009318-zt1o1bcz	27	2	,	,	,
cord-009318-zt1o1bcz	27	3	bulk	bulk	JJ
cord-009318-zt1o1bcz	27	4	reactions	reaction	NNS
cord-009318-zt1o1bcz	27	5	have	have	VBP
cord-009318-zt1o1bcz	27	6	three	three	CD
cord-009318-zt1o1bcz	27	7	important	important	JJ
cord-009318-zt1o1bcz	27	8	limitations	limitation	NNS
cord-009318-zt1o1bcz	27	9	with	with	IN
cord-009318-zt1o1bcz	27	10	regard	regard	NN
cord-009318-zt1o1bcz	27	11	to	to	IN
cord-009318-zt1o1bcz	27	12	assay	assay	NN
cord-009318-zt1o1bcz	27	13	optimization	optimization	NN
cord-009318-zt1o1bcz	27	14	:	:	:
cord-009318-zt1o1bcz	27	15	(	(	-LRB-
cord-009318-zt1o1bcz	27	16	i	i	NN
cord-009318-zt1o1bcz	27	17	)	)	-RRB-
cord-009318-zt1o1bcz	27	18	differences	difference	NNS
cord-009318-zt1o1bcz	27	19	in	in	IN
cord-009318-zt1o1bcz	27	20	the	the	DT
cord-009318-zt1o1bcz	27	21	kinetics	kinetic	NNS
cord-009318-zt1o1bcz	27	22	of	of	IN
cord-009318-zt1o1bcz	27	23	specific	specific	JJ
cord-009318-zt1o1bcz	27	24	and	and	CC
cord-009318-zt1o1bcz	27	25	non	non	JJ
cord-009318-zt1o1bcz	27	26	-	-	JJ
cord-009318-zt1o1bcz	27	27	specific	specific	JJ
cord-009318-zt1o1bcz	27	28	reactions	reaction	NNS
cord-009318-zt1o1bcz	27	29	can	can	MD
cord-009318-zt1o1bcz	27	30	not	not	RB
cord-009318-zt1o1bcz	27	31	be	be	VB
cord-009318-zt1o1bcz	27	32	separated	separate	VBN
cord-009318-zt1o1bcz	27	33	,	,	,
cord-009318-zt1o1bcz	28	1	(	(	-LRB-
cord-009318-zt1o1bcz	28	2	ii	ii	LS
cord-009318-zt1o1bcz	28	3	)	)	-RRB-
cord-009318-zt1o1bcz	28	4	rare	rare	JJ
cord-009318-zt1o1bcz	28	5	but	but	CC
cord-009318-zt1o1bcz	28	6	significant	significant	JJ
cord-009318-zt1o1bcz	28	7	events	event	NNS
cord-009318-zt1o1bcz	28	8	,	,	,
cord-009318-zt1o1bcz	28	9	such	such	JJ
cord-009318-zt1o1bcz	28	10	as	as	IN
cord-009318-zt1o1bcz	28	11	early	early	JJ
cord-009318-zt1o1bcz	28	12	but	but	CC
cord-009318-zt1o1bcz	28	13	infrequent	infrequent	JJ
cord-009318-zt1o1bcz	28	14	non	non	JJ
cord-009318-zt1o1bcz	28	15	-	-	JJ
cord-009318-zt1o1bcz	28	16	specific	specific	JJ
cord-009318-zt1o1bcz	28	17	amplification	amplification	NN
cord-009318-zt1o1bcz	28	18	,	,	,
cord-009318-zt1o1bcz	28	19	can	can	MD
cord-009318-zt1o1bcz	28	20	not	not	RB
cord-009318-zt1o1bcz	28	21	be	be	VB
cord-009318-zt1o1bcz	28	22	easily	easily	RB
cord-009318-zt1o1bcz	28	23	characterized	characterize	VBN
cord-009318-zt1o1bcz	28	24	;	;	:
cord-009318-zt1o1bcz	28	25	and	and	CC
cord-009318-zt1o1bcz	28	26	(	(	-LRB-
cord-009318-zt1o1bcz	28	27	iii	iii	NN
cord-009318-zt1o1bcz	28	28	)	)	-RRB-
cord-009318-zt1o1bcz	28	29	testing	test	VBG
cord-009318-zt1o1bcz	28	30	the	the	DT
cord-009318-zt1o1bcz	28	31	full	full	JJ
cord-009318-zt1o1bcz	28	32	design	design	NN
cord-009318-zt1o1bcz	28	33	space	space	NN
cord-009318-zt1o1bcz	28	34	requires	require	VBZ
cord-009318-zt1o1bcz	28	35	many	many	JJ
cord-009318-zt1o1bcz	28	36	hundreds	hundred	NNS
cord-009318-zt1o1bcz	28	37	of	of	IN
cord-009318-zt1o1bcz	28	38	replicates	replicate	NNS
cord-009318-zt1o1bcz	28	39	to	to	TO
cord-009318-zt1o1bcz	28	40	obtain	obtain	VB
cord-009318-zt1o1bcz	28	41	statistically	statistically	RB
cord-009318-zt1o1bcz	28	42	significant	significant	JJ
cord-009318-zt1o1bcz	28	43	data	datum	NNS
cord-009318-zt1o1bcz	28	44	.	.	.
cord-009318-zt1o1bcz	29	1	To	to	TO
cord-009318-zt1o1bcz	29	2	improve	improve	VB
cord-009318-zt1o1bcz	29	3	an	an	DT
cord-009318-zt1o1bcz	29	4	assay	assay	NN
cord-009318-zt1o1bcz	29	5	's	's	POS
cord-009318-zt1o1bcz	29	6	analytical	analytical	JJ
cord-009318-zt1o1bcz	29	7	specificity	specificity	NN
cord-009318-zt1o1bcz	29	8	and	and	CC
cord-009318-zt1o1bcz	29	9	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	29	10	,	,	,
cord-009318-zt1o1bcz	29	11	one	one	CD
cord-009318-zt1o1bcz	29	12	strategy	strategy	NN
cord-009318-zt1o1bcz	29	13	is	be	VBZ
cord-009318-zt1o1bcz	29	14	to	to	TO
cord-009318-zt1o1bcz	29	15	eliminate	eliminate	VB
cord-009318-zt1o1bcz	29	16	the	the	DT
cord-009318-zt1o1bcz	29	17	detection	detection	NN
cord-009318-zt1o1bcz	29	18	of	of	IN
cord-009318-zt1o1bcz	29	19	non	non	JJ
cord-009318-zt1o1bcz	29	20	-	-	JJ
cord-009318-zt1o1bcz	29	21	specific	specific	JJ
cord-009318-zt1o1bcz	29	22	amplification	amplification	NN
cord-009318-zt1o1bcz	29	23	.	.	.
cord-009318-zt1o1bcz	30	1	In	in	IN
cord-009318-zt1o1bcz	30	2	bulk	bulk	JJ
cord-009318-zt1o1bcz	30	3	LAMP	lamp	NN
cord-009318-zt1o1bcz	30	4	experiments	experiment	NNS
cord-009318-zt1o1bcz	30	5	,	,	,
cord-009318-zt1o1bcz	30	6	non	non	JJ
cord-009318-zt1o1bcz	30	7	-	-	JJ
cord-009318-zt1o1bcz	30	8	specific	specific	JJ
cord-009318-zt1o1bcz	30	9	amplification	amplification	NN
cord-009318-zt1o1bcz	30	10	can	can	MD
cord-009318-zt1o1bcz	30	11	be	be	VB
cord-009318-zt1o1bcz	30	12	excluded	exclude	VBN
cord-009318-zt1o1bcz	30	13	from	from	IN
cord-009318-zt1o1bcz	30	14	detection	detection	NN
cord-009318-zt1o1bcz	30	15	by	by	IN
cord-009318-zt1o1bcz	30	16	using	use	VBG
cord-009318-zt1o1bcz	30	17	probes	probe	NNS
cord-009318-zt1o1bcz	30	18	,	,	,
cord-009318-zt1o1bcz	30	19	beacons	beacon	NNS
cord-009318-zt1o1bcz	30	20	,	,	,
cord-009318-zt1o1bcz	30	21	FRET	fret	NN
cord-009318-zt1o1bcz	30	22	or	or	CC
cord-009318-zt1o1bcz	30	23	reporter	reporter	NN
cord-009318-zt1o1bcz	30	24	-	-	HYPH
cord-009318-zt1o1bcz	30	25	quencher	quencher	NN
cord-009318-zt1o1bcz	30	26	schemes	scheme	NNS
cord-009318-zt1o1bcz	30	27	that	that	WDT
cord-009318-zt1o1bcz	30	28	show	show	VBP
cord-009318-zt1o1bcz	30	29	only	only	RB
cord-009318-zt1o1bcz	30	30	specific	specific	JJ
cord-009318-zt1o1bcz	30	31	amplification	amplification	NN
cord-009318-zt1o1bcz	30	32	of	of	IN
cord-009318-zt1o1bcz	30	33	the	the	DT
cord-009318-zt1o1bcz	30	34	target	target	NN
cord-009318-zt1o1bcz	30	35	(	(	-LRB-
cord-009318-zt1o1bcz	30	36	10	10	CD
cord-009318-zt1o1bcz	30	37	)	)	-RRB-
cord-009318-zt1o1bcz	30	38	(	(	-LRB-
cord-009318-zt1o1bcz	30	39	11	11	CD
cord-009318-zt1o1bcz	30	40	)	)	-RRB-
cord-009318-zt1o1bcz	30	41	(	(	-LRB-
cord-009318-zt1o1bcz	30	42	12	12	CD
cord-009318-zt1o1bcz	30	43	)	)	-RRB-
cord-009318-zt1o1bcz	30	44	(	(	-LRB-
cord-009318-zt1o1bcz	30	45	13	13	CD
cord-009318-zt1o1bcz	30	46	)	)	-RRB-
cord-009318-zt1o1bcz	30	47	(	(	-LRB-
cord-009318-zt1o1bcz	30	48	14	14	CD
cord-009318-zt1o1bcz	30	49	)	)	-RRB-
cord-009318-zt1o1bcz	30	50	(	(	-LRB-
cord-009318-zt1o1bcz	30	51	15	15	CD
cord-009318-zt1o1bcz	30	52	)	)	-RRB-
cord-009318-zt1o1bcz	30	53	(	(	-LRB-
cord-009318-zt1o1bcz	30	54	16	16	CD
cord-009318-zt1o1bcz	30	55	)	)	-RRB-
cord-009318-zt1o1bcz	30	56	(	(	-LRB-
cord-009318-zt1o1bcz	30	57	17	17	CD
cord-009318-zt1o1bcz	30	58	)	)	-RRB-
cord-009318-zt1o1bcz	30	59	(	(	-LRB-
cord-009318-zt1o1bcz	30	60	18	18	CD
cord-009318-zt1o1bcz	30	61	)	)	-RRB-
cord-009318-zt1o1bcz	30	62	(	(	-LRB-
cord-009318-zt1o1bcz	30	63	19	19	CD
cord-009318-zt1o1bcz	30	64	)	)	-RRB-
cord-009318-zt1o1bcz	30	65	.	.	.
cord-009318-zt1o1bcz	31	1	Although	although	IN
cord-009318-zt1o1bcz	31	2	these	these	DT
cord-009318-zt1o1bcz	31	3	methods	method	NNS
cord-009318-zt1o1bcz	31	4	improve	improve	VBP
cord-009318-zt1o1bcz	31	5	the	the	DT
cord-009318-zt1o1bcz	31	6	assay	assay	NN
cord-009318-zt1o1bcz	31	7	,	,	,
cord-009318-zt1o1bcz	31	8	they	-PRON-	PRP
cord-009318-zt1o1bcz	31	9	do	do	VBP
cord-009318-zt1o1bcz	31	10	not	not	RB
cord-009318-zt1o1bcz	31	11	capture	capture	VB
cord-009318-zt1o1bcz	31	12	non	non	JJ
cord-009318-zt1o1bcz	31	13	-	-	JJ
cord-009318-zt1o1bcz	31	14	specific	specific	JJ
cord-009318-zt1o1bcz	31	15	reactions	reaction	NNS
cord-009318-zt1o1bcz	31	16	and	and	CC
cord-009318-zt1o1bcz	31	17	thus	thus	RB
cord-009318-zt1o1bcz	31	18	can	can	MD
cord-009318-zt1o1bcz	31	19	not	not	RB
cord-009318-zt1o1bcz	31	20	give	give	VB
cord-009318-zt1o1bcz	31	21	insights	insight	NNS
cord-009318-zt1o1bcz	31	22	into	into	IN
cord-009318-zt1o1bcz	31	23	the	the	DT
cord-009318-zt1o1bcz	31	24	origin	origin	NN
cord-009318-zt1o1bcz	31	25	of	of	IN
cord-009318-zt1o1bcz	31	26	non	non	JJ
cord-009318-zt1o1bcz	31	27	-	-	JJ
cord-009318-zt1o1bcz	31	28	specific	specific	JJ
cord-009318-zt1o1bcz	31	29	amplification	amplification	NN
cord-009318-zt1o1bcz	31	30	or	or	CC
cord-009318-zt1o1bcz	31	31	the	the	DT
cord-009318-zt1o1bcz	31	32	conditions	condition	NNS
cord-009318-zt1o1bcz	31	33	that	that	WDT
cord-009318-zt1o1bcz	31	34	led	lead	VBD
cord-009318-zt1o1bcz	31	35	to	to	IN
cord-009318-zt1o1bcz	31	36	non	non	JJ
cord-009318-zt1o1bcz	31	37	-	-	JJ
cord-009318-zt1o1bcz	31	38	specific	specific	JJ
cord-009318-zt1o1bcz	31	39	amplicons	amplicon	NNS
cord-009318-zt1o1bcz	31	40	.	.	.
cord-009318-zt1o1bcz	32	1	Moreover	moreover	RB
cord-009318-zt1o1bcz	32	2	,	,	,
cord-009318-zt1o1bcz	32	3	probes	probe	NNS
cord-009318-zt1o1bcz	32	4	and	and	CC
cord-009318-zt1o1bcz	32	5	beacons	beacon	NNS
cord-009318-zt1o1bcz	32	6	do	do	VBP
cord-009318-zt1o1bcz	32	7	not	not	RB
cord-009318-zt1o1bcz	32	8	eliminate	eliminate	VB
cord-009318-zt1o1bcz	32	9	non	non	JJ
cord-009318-zt1o1bcz	32	10	-	-	JJ
cord-009318-zt1o1bcz	32	11	specific	specific	JJ
cord-009318-zt1o1bcz	32	12	amplification	amplification	NN
cord-009318-zt1o1bcz	32	13	;	;	:
cord-009318-zt1o1bcz	32	14	non	non	JJ
cord-009318-zt1o1bcz	32	15	-	-	JJ
cord-009318-zt1o1bcz	32	16	specific	specific	JJ
cord-009318-zt1o1bcz	32	17	amplification	amplification	NN
cord-009318-zt1o1bcz	32	18	still	still	RB
cord-009318-zt1o1bcz	32	19	competes	compete	VBZ
cord-009318-zt1o1bcz	32	20	for	for	IN
cord-009318-zt1o1bcz	32	21	reagents	reagent	NNS
cord-009318-zt1o1bcz	32	22	and	and	CC
cord-009318-zt1o1bcz	32	23	can	can	MD
cord-009318-zt1o1bcz	32	24	limit	limit	VB
cord-009318-zt1o1bcz	32	25	the	the	DT
cord-009318-zt1o1bcz	32	26	extent	extent	NN
cord-009318-zt1o1bcz	32	27	of	of	IN
cord-009318-zt1o1bcz	32	28	the	the	DT
cord-009318-zt1o1bcz	32	29	signal	signal	NN
cord-009318-zt1o1bcz	32	30	generated	generate	VBN
cord-009318-zt1o1bcz	32	31	by	by	IN
cord-009318-zt1o1bcz	32	32	specific	specific	JJ
cord-009318-zt1o1bcz	32	33	amplification	amplification	NN
cord-009318-zt1o1bcz	32	34	events	event	NNS
cord-009318-zt1o1bcz	32	35	(	(	-LRB-
cord-009318-zt1o1bcz	32	36	20	20	CD
cord-009318-zt1o1bcz	32	37	)	)	-RRB-
cord-009318-zt1o1bcz	32	38	.	.	.
cord-009318-zt1o1bcz	33	1	Hence	hence	RB
cord-009318-zt1o1bcz	33	2	,	,	,
cord-009318-zt1o1bcz	33	3	it	-PRON-	PRP
cord-009318-zt1o1bcz	33	4	is	be	VBZ
cord-009318-zt1o1bcz	33	5	highly	highly	RB
cord-009318-zt1o1bcz	33	6	desirable	desirable	JJ
cord-009318-zt1o1bcz	33	7	to	to	TO
cord-009318-zt1o1bcz	33	8	distinguish	distinguish	VB
cord-009318-zt1o1bcz	33	9	specific	specific	JJ
cord-009318-zt1o1bcz	33	10	from	from	IN
cord-009318-zt1o1bcz	33	11	non	non	JJ
cord-009318-zt1o1bcz	33	12	-	-	JJ
cord-009318-zt1o1bcz	33	13	specific	specific	JJ
cord-009318-zt1o1bcz	33	14	amplification	amplification	NN
cord-009318-zt1o1bcz	33	15	.	.	.
cord-009318-zt1o1bcz	34	1	In	in	IN
cord-009318-zt1o1bcz	34	2	this	this	DT
cord-009318-zt1o1bcz	34	3	study	study	NN
cord-009318-zt1o1bcz	34	4	,	,	,
cord-009318-zt1o1bcz	34	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	34	6	combined	combine	VBD
cord-009318-zt1o1bcz	34	7	sequencing	sequencing	NN
cord-009318-zt1o1bcz	34	8	and	and	CC
cord-009318-zt1o1bcz	34	9	digital	digital	JJ
cord-009318-zt1o1bcz	34	10	singlemolecule	singlemolecule	NNP
cord-009318-zt1o1bcz	34	11	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	34	12	(	(	-LRB-
cord-009318-zt1o1bcz	34	13	dLAMP	dLAMP	NNP
cord-009318-zt1o1bcz	34	14	)	)	-RRB-
cord-009318-zt1o1bcz	34	15	with	with	IN
cord-009318-zt1o1bcz	34	16	high	high	JJ
cord-009318-zt1o1bcz	34	17	-	-	HYPH
cord-009318-zt1o1bcz	34	18	resolution	resolution	NN
cord-009318-zt1o1bcz	34	19	melting	melting	NN
cord-009318-zt1o1bcz	34	20	temperature	temperature	NN
cord-009318-zt1o1bcz	34	21	(	(	-LRB-
cord-009318-zt1o1bcz	34	22	HRM	HRM	NNP
cord-009318-zt1o1bcz	34	23	)	)	-RRB-
cord-009318-zt1o1bcz	34	24	to	to	TO
cord-009318-zt1o1bcz	34	25	probe	probe	VB
cord-009318-zt1o1bcz	34	26	the	the	DT
cord-009318-zt1o1bcz	34	27	fundamental	fundamental	JJ
cord-009318-zt1o1bcz	34	28	mechanics	mechanic	NNS
cord-009318-zt1o1bcz	34	29	of	of	IN
cord-009318-zt1o1bcz	34	30	amplification	amplification	NN
cord-009318-zt1o1bcz	34	31	reactions	reaction	NNS
cord-009318-zt1o1bcz	34	32	.	.	.
cord-009318-zt1o1bcz	35	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	35	2	used	use	VBD
cord-009318-zt1o1bcz	35	3	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	35	4	to	to	TO
cord-009318-zt1o1bcz	35	5	extract	extract	VB
cord-009318-zt1o1bcz	35	6	real	real	JJ
cord-009318-zt1o1bcz	35	7	-	-	HYPH
cord-009318-zt1o1bcz	35	8	time	time	NN
cord-009318-zt1o1bcz	35	9	kinetic	kinetic	JJ
cord-009318-zt1o1bcz	35	10	information	information	NN
cord-009318-zt1o1bcz	35	11	to	to	TO
cord-009318-zt1o1bcz	35	12	identify	identify	VB
cord-009318-zt1o1bcz	35	13	the	the	DT
cord-009318-zt1o1bcz	35	14	digital	digital	JJ
cord-009318-zt1o1bcz	35	15	threshold	threshold	NN
cord-009318-zt1o1bcz	35	16	data	data	NN
cord-009318-zt1o1bcz	35	17	-	-	HYPH
cord-009318-zt1o1bcz	35	18	processing	processing	NN
cord-009318-zt1o1bcz	35	19	parameters	parameter	NNS
cord-009318-zt1o1bcz	35	20	that	that	WDT
cord-009318-zt1o1bcz	35	21	minimize	minimize	VBP
cord-009318-zt1o1bcz	35	22	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	35	23	amplification	amplification	NN
cord-009318-zt1o1bcz	35	24	events	event	NNS
cord-009318-zt1o1bcz	35	25	and	and	CC
cord-009318-zt1o1bcz	35	26	elucidate	elucidate	VB
cord-009318-zt1o1bcz	35	27	how	how	WRB
cord-009318-zt1o1bcz	35	28	an	an	DT
cord-009318-zt1o1bcz	35	29	interfering	interfere	VBG
cord-009318-zt1o1bcz	35	30	molecule	molecule	NN
cord-009318-zt1o1bcz	35	31	impacts	impact	NNS
cord-009318-zt1o1bcz	35	32	amplification	amplification	NN
cord-009318-zt1o1bcz	35	33	.	.	.
cord-009318-zt1o1bcz	36	1	Digital	digital	JJ
cord-009318-zt1o1bcz	36	2	single	single	JJ
cord-009318-zt1o1bcz	36	3	-	-	HYPH
cord-009318-zt1o1bcz	36	4	molecule	molecule	NN
cord-009318-zt1o1bcz	36	5	methods	method	NNS
cord-009318-zt1o1bcz	36	6	separating	separate	VBG
cord-009318-zt1o1bcz	36	7	individual	individual	JJ
cord-009318-zt1o1bcz	36	8	amplification	amplification	NN
cord-009318-zt1o1bcz	36	9	events	event	NNS
cord-009318-zt1o1bcz	36	10	into	into	IN
cord-009318-zt1o1bcz	36	11	discrete	discrete	JJ
cord-009318-zt1o1bcz	36	12	compartments	compartment	NNS
cord-009318-zt1o1bcz	36	13	,	,	,
cord-009318-zt1o1bcz	36	14	eliminating	eliminate	VBG
cord-009318-zt1o1bcz	36	15	interference	interference	NN
cord-009318-zt1o1bcz	36	16	among	among	IN
cord-009318-zt1o1bcz	36	17	individual	individual	JJ
cord-009318-zt1o1bcz	36	18	amplification	amplification	NN
cord-009318-zt1o1bcz	36	19	events	event	NNS
cord-009318-zt1o1bcz	36	20	(	(	-LRB-
cord-009318-zt1o1bcz	36	21	21	21	CD
cord-009318-zt1o1bcz	36	22	,	,	,
cord-009318-zt1o1bcz	36	23	22	22	CD
cord-009318-zt1o1bcz	36	24	)	)	-RRB-
cord-009318-zt1o1bcz	36	25	.	.	.
cord-009318-zt1o1bcz	37	1	Furthermore	furthermore	RB
cord-009318-zt1o1bcz	37	2	,	,	,
cord-009318-zt1o1bcz	37	3	digital	digital	JJ
cord-009318-zt1o1bcz	37	4	experiments	experiment	NNS
cord-009318-zt1o1bcz	37	5	consist	consist	VBP
cord-009318-zt1o1bcz	37	6	of	of	IN
cord-009318-zt1o1bcz	37	7	thousands	thousand	NNS
cord-009318-zt1o1bcz	37	8	of	of	IN
cord-009318-zt1o1bcz	37	9	reactions	reaction	NNS
cord-009318-zt1o1bcz	37	10	that	that	WDT
cord-009318-zt1o1bcz	37	11	run	run	VBP
cord-009318-zt1o1bcz	37	12	in	in	IN
cord-009318-zt1o1bcz	37	13	parallel	parallel	NN
cord-009318-zt1o1bcz	37	14	and	and	CC
cord-009318-zt1o1bcz	37	15	thus	thus	RB
cord-009318-zt1o1bcz	37	16	provide	provide	VB
cord-009318-zt1o1bcz	37	17	valuable	valuable	JJ
cord-009318-zt1o1bcz	37	18	statistical	statistical	JJ
cord-009318-zt1o1bcz	37	19	information	information	NN
cord-009318-zt1o1bcz	37	20	(	(	-LRB-
cord-009318-zt1o1bcz	37	21	21	21	CD
cord-009318-zt1o1bcz	37	22	)	)	-RRB-
cord-009318-zt1o1bcz	37	23	(	(	-LRB-
cord-009318-zt1o1bcz	37	24	22	22	CD
cord-009318-zt1o1bcz	37	25	)	)	-RRB-
cord-009318-zt1o1bcz	37	26	(	(	-LRB-
cord-009318-zt1o1bcz	37	27	23	23	CD
cord-009318-zt1o1bcz	37	28	)	)	-RRB-
cord-009318-zt1o1bcz	37	29	.	.	.
cord-009318-zt1o1bcz	38	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	38	2	used	use	VBD
cord-009318-zt1o1bcz	38	3	real	real	JJ
cord-009318-zt1o1bcz	38	4	-	-	HYPH
cord-009318-zt1o1bcz	38	5	time	time	NN
cord-009318-zt1o1bcz	38	6	imaging	imaging	NN
cord-009318-zt1o1bcz	38	7	to	to	TO
cord-009318-zt1o1bcz	38	8	monitor	monitor	VB
cord-009318-zt1o1bcz	38	9	the	the	DT
cord-009318-zt1o1bcz	38	10	kinetics	kinetic	NNS
cord-009318-zt1o1bcz	38	11	of	of	IN
cord-009318-zt1o1bcz	38	12	20,000	20,000	CD
cord-009318-zt1o1bcz	38	13	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	38	14	reactions	reaction	NNS
cord-009318-zt1o1bcz	38	15	per	per	IN
cord-009318-zt1o1bcz	38	16	experiment	experiment	NN
cord-009318-zt1o1bcz	38	17	and	and	CC
cord-009318-zt1o1bcz	38	18	observe	observe	VB
cord-009318-zt1o1bcz	38	19	∼1.2	∼1.2	NNP
cord-009318-zt1o1bcz	38	20	×	×	NNP
cord-009318-zt1o1bcz	38	21	10	10	CD
cord-009318-zt1o1bcz	38	22	6	6	CD
cord-009318-zt1o1bcz	38	23	reactions	reaction	NNS
cord-009318-zt1o1bcz	38	24	in	in	IN
cord-009318-zt1o1bcz	38	25	total	total	NN
cord-009318-zt1o1bcz	38	26	.	.	.
cord-009318-zt1o1bcz	39	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	39	2	hypothesized	hypothesize	VBD
cord-009318-zt1o1bcz	39	3	that	that	IN
cord-009318-zt1o1bcz	39	4	highresolution	highresolution	NN
cord-009318-zt1o1bcz	39	5	melting	melt	VBG
cord-009318-zt1o1bcz	39	6	analysis	analysis	NN
cord-009318-zt1o1bcz	39	7	(	(	-LRB-
cord-009318-zt1o1bcz	39	8	HRM	HRM	NNP
cord-009318-zt1o1bcz	39	9	)	)	-RRB-
cord-009318-zt1o1bcz	39	10	could	could	MD
cord-009318-zt1o1bcz	39	11	be	be	VB
cord-009318-zt1o1bcz	39	12	a	a	DT
cord-009318-zt1o1bcz	39	13	tool	tool	NN
cord-009318-zt1o1bcz	39	14	for	for	IN
cord-009318-zt1o1bcz	39	15	separating	separate	VBG
cord-009318-zt1o1bcz	39	16	specific	specific	JJ
cord-009318-zt1o1bcz	39	17	from	from	IN
cord-009318-zt1o1bcz	39	18	non	non	JJ
cord-009318-zt1o1bcz	39	19	-	-	JJ
cord-009318-zt1o1bcz	39	20	specific	specific	JJ
cord-009318-zt1o1bcz	39	21	amplification	amplification	NN
cord-009318-zt1o1bcz	39	22	events	event	NNS
cord-009318-zt1o1bcz	39	23	and	and	CC
cord-009318-zt1o1bcz	39	24	for	for	IN
cord-009318-zt1o1bcz	39	25	identifying	identify	VBG
cord-009318-zt1o1bcz	39	26	the	the	DT
cord-009318-zt1o1bcz	39	27	optimal	optimal	JJ
cord-009318-zt1o1bcz	39	28	digital	digital	JJ
cord-009318-zt1o1bcz	39	29	threshold	threshold	NN
cord-009318-zt1o1bcz	39	30	data	data	NN
cord-009318-zt1o1bcz	39	31	-	-	HYPH
cord-009318-zt1o1bcz	39	32	processing	processing	NN
cord-009318-zt1o1bcz	39	33	parameters	parameter	NNS
cord-009318-zt1o1bcz	39	34	to	to	TO
cord-009318-zt1o1bcz	39	35	distinguish	distinguish	VB
cord-009318-zt1o1bcz	39	36	specific	specific	JJ
cord-009318-zt1o1bcz	39	37	and	and	CC
cord-009318-zt1o1bcz	39	38	non	non	JJ
cord-009318-zt1o1bcz	39	39	-	-	JJ
cord-009318-zt1o1bcz	39	40	specific	specific	JJ
cord-009318-zt1o1bcz	39	41	amplification	amplification	NN
cord-009318-zt1o1bcz	39	42	events	event	NNS
cord-009318-zt1o1bcz	39	43	(	(	-LRB-
cord-009318-zt1o1bcz	39	44	even	even	RB
cord-009318-zt1o1bcz	39	45	when	when	WRB
cord-009318-zt1o1bcz	39	46	an	an	DT
cord-009318-zt1o1bcz	39	47	assay	assay	NN
cord-009318-zt1o1bcz	39	48	is	be	VBZ
cord-009318-zt1o1bcz	39	49	deployed	deploy	VBN
cord-009318-zt1o1bcz	39	50	without	without	IN
cord-009318-zt1o1bcz	39	51	HRM	HRM	NNP
cord-009318-zt1o1bcz	39	52	)	)	-RRB-
cord-009318-zt1o1bcz	39	53	.	.	.
cord-009318-zt1o1bcz	40	1	To	to	TO
cord-009318-zt1o1bcz	40	2	test	test	VB
cord-009318-zt1o1bcz	40	3	this	this	DT
cord-009318-zt1o1bcz	40	4	hypothesis	hypothesis	NN
cord-009318-zt1o1bcz	40	5	,	,	,
cord-009318-zt1o1bcz	40	6	we	-PRON-	PRP
cord-009318-zt1o1bcz	40	7	used	use	VBD
cord-009318-zt1o1bcz	40	8	a	a	DT
cord-009318-zt1o1bcz	40	9	dLAMP	dlamp	NN
cord-009318-zt1o1bcz	40	10	assay	assay	NN
cord-009318-zt1o1bcz	40	11	with	with	IN
cord-009318-zt1o1bcz	40	12	CT	CT	NNP
cord-009318-zt1o1bcz	40	13	DNA	DNA	NNP
cord-009318-zt1o1bcz	40	14	as	as	IN
cord-009318-zt1o1bcz	40	15	the	the	DT
cord-009318-zt1o1bcz	40	16	target	target	NN
cord-009318-zt1o1bcz	40	17	(	(	-LRB-
cord-009318-zt1o1bcz	40	18	combined	combine	VBN
cord-009318-zt1o1bcz	40	19	with	with	IN
cord-009318-zt1o1bcz	40	20	sequencing	sequencing	NN
cord-009318-zt1o1bcz	40	21	to	to	TO
cord-009318-zt1o1bcz	40	22	identify	identify	VB
cord-009318-zt1o1bcz	40	23	the	the	DT
cord-009318-zt1o1bcz	40	24	products	product	NNS
cord-009318-zt1o1bcz	40	25	of	of	IN
cord-009318-zt1o1bcz	40	26	bulk	bulk	JJ
cord-009318-zt1o1bcz	40	27	reactions	reaction	NNS
cord-009318-zt1o1bcz	40	28	)	)	-RRB-
cord-009318-zt1o1bcz	40	29	to	to	TO
cord-009318-zt1o1bcz	40	30	analyze	analyze	VB
cord-009318-zt1o1bcz	40	31	both	both	DT
cord-009318-zt1o1bcz	40	32	specific	specific	JJ
cord-009318-zt1o1bcz	40	33	and	and	CC
cord-009318-zt1o1bcz	40	34	non	non	JJ
cord-009318-zt1o1bcz	40	35	-	-	JJ
cord-009318-zt1o1bcz	40	36	specific	specific	JJ
cord-009318-zt1o1bcz	40	37	amplification	amplification	NN
cord-009318-zt1o1bcz	40	38	under	under	IN
cord-009318-zt1o1bcz	40	39	conditions	condition	NNS
cord-009318-zt1o1bcz	40	40	that	that	WDT
cord-009318-zt1o1bcz	40	41	include	include	VBP
cord-009318-zt1o1bcz	40	42	clinically	clinically	RB
cord-009318-zt1o1bcz	40	43	relevant	relevant	JJ
cord-009318-zt1o1bcz	40	44	concentrations	concentration	NNS
cord-009318-zt1o1bcz	40	45	of	of	IN
cord-009318-zt1o1bcz	40	46	background	background	NN
cord-009318-zt1o1bcz	40	47	human	human	JJ
cord-009318-zt1o1bcz	40	48	DNA	dna	NN
cord-009318-zt1o1bcz	40	49	.	.	.
cord-009318-zt1o1bcz	41	1	IsoAmp	isoamp	NN
cord-009318-zt1o1bcz	42	1	I	-PRON-	PRP
cord-009318-zt1o1bcz	42	2	(	(	-LRB-
cord-009318-zt1o1bcz	42	3	#	#	$
cord-009318-zt1o1bcz	42	4	B0537S	B0537S	NNP
cord-009318-zt1o1bcz	42	5	)	)	-RRB-
cord-009318-zt1o1bcz	42	6	,	,	,
cord-009318-zt1o1bcz	42	7	IsoAmp	IsoAmp	NNP
cord-009318-zt1o1bcz	42	8	II	II	NNP
cord-009318-zt1o1bcz	42	9	(	(	-LRB-
cord-009318-zt1o1bcz	42	10	#	#	$
cord-009318-zt1o1bcz	42	11	B0374S	B0374S	NNP
cord-009318-zt1o1bcz	42	12	)	)	-RRB-
cord-009318-zt1o1bcz	42	13	,	,	,
cord-009318-zt1o1bcz	42	14	MgSO	MgSO	NNP
cord-009318-zt1o1bcz	42	15	4	4	CD
cord-009318-zt1o1bcz	42	16	(	(	-LRB-
cord-009318-zt1o1bcz	42	17	#	#	$
cord-009318-zt1o1bcz	42	18	B1003S	B1003S	NNP
cord-009318-zt1o1bcz	42	19	)	)	-RRB-
cord-009318-zt1o1bcz	42	20	,	,	,
cord-009318-zt1o1bcz	42	21	deoxynucleotide	deoxynucleotide	JJ
cord-009318-zt1o1bcz	42	22	solution	solution	NN
cord-009318-zt1o1bcz	42	23	(	(	-LRB-
cord-009318-zt1o1bcz	42	24	#	#	$
cord-009318-zt1o1bcz	42	25	N0447S	N0447S	NNP
cord-009318-zt1o1bcz	42	26	)	)	-RRB-
cord-009318-zt1o1bcz	42	27	,	,	,
cord-009318-zt1o1bcz	42	28	Bovine	Bovine	NNP
cord-009318-zt1o1bcz	42	29	Serum	Serum	NNP
cord-009318-zt1o1bcz	42	30	Albumen	Albumen	NNP
cord-009318-zt1o1bcz	42	31	(	(	-LRB-
cord-009318-zt1o1bcz	42	32	BSA	BSA	NNP
cord-009318-zt1o1bcz	42	33	,	,	,
cord-009318-zt1o1bcz	42	34	#	#	NN
cord-009318-zt1o1bcz	42	35	B9000S0	b9000s0	NN
cord-009318-zt1o1bcz	42	36	)	)	-RRB-
cord-009318-zt1o1bcz	42	37	,	,	,
cord-009318-zt1o1bcz	42	38	Bst	Bst	NNP
cord-009318-zt1o1bcz	42	39	2.0	2.0	CD
cord-009318-zt1o1bcz	42	40	(	(	-LRB-
cord-009318-zt1o1bcz	42	41	8,000	8,000	CD
cord-009318-zt1o1bcz	42	42	U	U	NNP
cord-009318-zt1o1bcz	42	43	/	/	SYM
cord-009318-zt1o1bcz	42	44	ml	ml	NNS
cord-009318-zt1o1bcz	42	45	,	,	,
cord-009318-zt1o1bcz	42	46	#	#	NNP
cord-009318-zt1o1bcz	42	47	M0537S	M0537S	NNP
cord-009318-zt1o1bcz	42	48	)	)	-RRB-
cord-009318-zt1o1bcz	42	49	and	and	CC
cord-009318-zt1o1bcz	42	50	Bst	Bst	NNP
cord-009318-zt1o1bcz	42	51	3.0	3.0	NNP
cord-009318-zt1o1bcz	42	52	(	(	-LRB-
cord-009318-zt1o1bcz	42	53	8000	8000	CD
cord-009318-zt1o1bcz	42	54	U	u	NN
cord-009318-zt1o1bcz	42	55	/	/	SYM
cord-009318-zt1o1bcz	42	56	ml	ml	NN
cord-009318-zt1o1bcz	42	57	,	,	,
cord-009318-zt1o1bcz	42	58	#	#	NN
cord-009318-zt1o1bcz	42	59	M0374S	M0374S	NNP
cord-009318-zt1o1bcz	42	60	)	)	-RRB-
cord-009318-zt1o1bcz	42	61	were	be	VBD
cord-009318-zt1o1bcz	42	62	purchased	purchase	VBN
cord-009318-zt1o1bcz	42	63	from	from	IN
cord-009318-zt1o1bcz	42	64	New	New	NNP
cord-009318-zt1o1bcz	42	65	England	England	NNP
cord-009318-zt1o1bcz	42	66	Biolabs	Biolabs	NNP
cord-009318-zt1o1bcz	42	67	(	(	-LRB-
cord-009318-zt1o1bcz	42	68	Ipswich	Ipswich	NNP
cord-009318-zt1o1bcz	42	69	,	,	,
cord-009318-zt1o1bcz	42	70	MA	MA	NNP
cord-009318-zt1o1bcz	42	71	,	,	,
cord-009318-zt1o1bcz	42	72	USA	USA	NNP
cord-009318-zt1o1bcz	42	73	)	)	-RRB-
cord-009318-zt1o1bcz	42	74	.	.	.
cord-009318-zt1o1bcz	43	1	Ambion	Ambion	NNP
cord-009318-zt1o1bcz	43	2	RNase	rnase	NN
cord-009318-zt1o1bcz	43	3	Cocktail	cocktail	NN
cord-009318-zt1o1bcz	43	4	(	(	-LRB-
cord-009318-zt1o1bcz	43	5	#	#	$
cord-009318-zt1o1bcz	43	6	AM2286	am2286	NN
cord-009318-zt1o1bcz	43	7	)	)	-RRB-
cord-009318-zt1o1bcz	43	8	,	,	,
cord-009318-zt1o1bcz	43	9	Ambion	Ambion	NNP
cord-009318-zt1o1bcz	43	10	nucleasefree	nucleasefree	JJ
cord-009318-zt1o1bcz	43	11	water	water	NN
cord-009318-zt1o1bcz	43	12	(	(	-LRB-
cord-009318-zt1o1bcz	43	13	#	#	$
cord-009318-zt1o1bcz	43	14	AM9932	am9932	NN
cord-009318-zt1o1bcz	43	15	)	)	-RRB-
cord-009318-zt1o1bcz	43	16	,	,	,
cord-009318-zt1o1bcz	43	17	Invitrogen	Invitrogen	NNP
cord-009318-zt1o1bcz	43	18	SYTO	SYTO	NNP
cord-009318-zt1o1bcz	43	19	9	9	CD
cord-009318-zt1o1bcz	43	20	(	(	-LRB-
cord-009318-zt1o1bcz	43	21	S34854	S34854	NNP
cord-009318-zt1o1bcz	43	22	)	)	-RRB-
cord-009318-zt1o1bcz	43	23	and	and	CC
cord-009318-zt1o1bcz	43	24	Invitrogen	Invitrogen	NNP
cord-009318-zt1o1bcz	43	25	ROX	ROX	NNP
cord-009318-zt1o1bcz	43	26	Reference	reference	NN
cord-009318-zt1o1bcz	43	27	Dye	dye	NN
cord-009318-zt1o1bcz	43	28	(	(	-LRB-
cord-009318-zt1o1bcz	43	29	#	#	$
cord-009318-zt1o1bcz	43	30	12223012	12223012	CD
cord-009318-zt1o1bcz	43	31	)	)	-RRB-
cord-009318-zt1o1bcz	43	32	were	be	VBD
cord-009318-zt1o1bcz	43	33	purchased	purchase	VBN
cord-009318-zt1o1bcz	43	34	from	from	IN
cord-009318-zt1o1bcz	43	35	Thermo	Thermo	NNP
cord-009318-zt1o1bcz	43	36	Fisher	Fisher	NNP
cord-009318-zt1o1bcz	43	37	Scientific	Scientific	NNP
cord-009318-zt1o1bcz	43	38	(	(	-LRB-
cord-009318-zt1o1bcz	43	39	Waltham	Waltham	NNP
cord-009318-zt1o1bcz	43	40	,	,	,
cord-009318-zt1o1bcz	43	41	MA	MA	NNP
cord-009318-zt1o1bcz	43	42	,	,	,
cord-009318-zt1o1bcz	43	43	USA	USA	NNP
cord-009318-zt1o1bcz	43	44	)	)	-RRB-
cord-009318-zt1o1bcz	43	45	.	.	.
cord-009318-zt1o1bcz	44	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	44	2	found	find	VBD
cord-009318-zt1o1bcz	44	3	it	-PRON-	PRP
cord-009318-zt1o1bcz	44	4	important	important	JJ
cord-009318-zt1o1bcz	44	5	to	to	TO
cord-009318-zt1o1bcz	44	6	use	use	VB
cord-009318-zt1o1bcz	44	7	SYTO	SYTO	NNP
cord-009318-zt1o1bcz	44	8	9	9	CD
cord-009318-zt1o1bcz	44	9	dilutions	dilution	NNS
cord-009318-zt1o1bcz	44	10	within	within	IN
cord-009318-zt1o1bcz	44	11	one	one	CD
cord-009318-zt1o1bcz	44	12	week	week	NN
cord-009318-zt1o1bcz	44	13	of	of	IN
cord-009318-zt1o1bcz	44	14	preparation	preparation	NN
cord-009318-zt1o1bcz	44	15	.	.	.
cord-009318-zt1o1bcz	45	1	Primers	primer	NNS
cord-009318-zt1o1bcz	45	2	sequences	sequence	NNS
cord-009318-zt1o1bcz	45	3	were	be	VBD
cord-009318-zt1o1bcz	45	4	targeted	target	VBN
cord-009318-zt1o1bcz	45	5	against	against	IN
cord-009318-zt1o1bcz	45	6	the	the	DT
cord-009318-zt1o1bcz	45	7	Chlamydia	Chlamydia	NNP
cord-009318-zt1o1bcz	45	8	trachomatis	trachomatis	NN
cord-009318-zt1o1bcz	45	9	23S	23S	NNP
cord-009318-zt1o1bcz	45	10	ribosomal	ribosomal	NN
cord-009318-zt1o1bcz	45	11	gene	gene	NN
cord-009318-zt1o1bcz	45	12	using	use	VBG
cord-009318-zt1o1bcz	45	13	Primer	Primer	NNP
cord-009318-zt1o1bcz	45	14	Explorer	Explorer	NNP
cord-009318-zt1o1bcz	45	15	V5	V5	NNP
cord-009318-zt1o1bcz	45	16	(	(	-LRB-
cord-009318-zt1o1bcz	45	17	Eiken	Eiken	NNP
cord-009318-zt1o1bcz	45	18	Chemical	Chemical	NNP
cord-009318-zt1o1bcz	45	19	,	,	,
cord-009318-zt1o1bcz	45	20	Tokyo	Tokyo	NNP
cord-009318-zt1o1bcz	45	21	,	,	,
cord-009318-zt1o1bcz	45	22	Japan	Japan	NNP
cord-009318-zt1o1bcz	45	23	)	)	-RRB-
cord-009318-zt1o1bcz	45	24	and	and	CC
cord-009318-zt1o1bcz	45	25	checked	check	VBD
cord-009318-zt1o1bcz	45	26	in	in	IN
cord-009318-zt1o1bcz	45	27	Snap	Snap	NNP
cord-009318-zt1o1bcz	45	28	-	-	HYPH
cord-009318-zt1o1bcz	45	29	Gene	gene	NN
cord-009318-zt1o1bcz	45	30	(	(	-LRB-
cord-009318-zt1o1bcz	45	31	GSL	GSL	NNP
cord-009318-zt1o1bcz	45	32	Biotech	Biotech	NNP
cord-009318-zt1o1bcz	45	33	,	,	,
cord-009318-zt1o1bcz	45	34	Chicago	Chicago	NNP
cord-009318-zt1o1bcz	45	35	,	,	,
cord-009318-zt1o1bcz	45	36	IL	IL	NNP
cord-009318-zt1o1bcz	45	37	,	,	,
cord-009318-zt1o1bcz	45	38	USA	USA	NNP
cord-009318-zt1o1bcz	45	39	)	)	-RRB-
cord-009318-zt1o1bcz	45	40	to	to	TO
cord-009318-zt1o1bcz	45	41	ensure	ensure	VB
cord-009318-zt1o1bcz	45	42	the	the	DT
cord-009318-zt1o1bcz	45	43	sequences	sequence	NNS
cord-009318-zt1o1bcz	45	44	were	be	VBD
cord-009318-zt1o1bcz	45	45	in	in	IN
cord-009318-zt1o1bcz	45	46	a	a	DT
cord-009318-zt1o1bcz	45	47	mutation	mutation	NN
cord-009318-zt1o1bcz	45	48	free	free	JJ
cord-009318-zt1o1bcz	45	49	region	region	NN
cord-009318-zt1o1bcz	45	50	from	from	IN
cord-009318-zt1o1bcz	45	51	the	the	DT
cord-009318-zt1o1bcz	45	52	available	available	JJ
cord-009318-zt1o1bcz	45	53	Genebank	Genebank	NNP
cord-009318-zt1o1bcz	45	54	sequences	sequence	NNS
cord-009318-zt1o1bcz	45	55	of	of	IN
cord-009318-zt1o1bcz	45	56	CT	CT	NNP
cord-009318-zt1o1bcz	45	57	.	.	.
cord-009318-zt1o1bcz	46	1	Primers	primer	NNS
cord-009318-zt1o1bcz	46	2	were	be	VBD
cord-009318-zt1o1bcz	46	3	purchased	purchase	VBN
cord-009318-zt1o1bcz	46	4	from	from	IN
cord-009318-zt1o1bcz	46	5	For	for	IN
cord-009318-zt1o1bcz	46	6	both	both	DT
cord-009318-zt1o1bcz	46	7	enzymes	enzyme	NNS
cord-009318-zt1o1bcz	46	8	,	,	,
cord-009318-zt1o1bcz	46	9	after	after	IN
cord-009318-zt1o1bcz	46	10	90	90	CD
cord-009318-zt1o1bcz	46	11	min	min	NN
cord-009318-zt1o1bcz	46	12	of	of	IN
cord-009318-zt1o1bcz	46	13	amplification	amplification	NN
cord-009318-zt1o1bcz	46	14	,	,	,
cord-009318-zt1o1bcz	46	15	reactions	reaction	NNS
cord-009318-zt1o1bcz	46	16	were	be	VBD
cord-009318-zt1o1bcz	46	17	ramped	ramp	VBN
cord-009318-zt1o1bcz	46	18	to	to	IN
cord-009318-zt1o1bcz	46	19	95	95	CD
cord-009318-zt1o1bcz	46	20	•	•	NN
cord-009318-zt1o1bcz	46	21	C	c	NN
cord-009318-zt1o1bcz	46	22	at	at	IN
cord-009318-zt1o1bcz	46	23	maximum	maximum	JJ
cord-009318-zt1o1bcz	46	24	output	output	NN
cord-009318-zt1o1bcz	46	25	and	and	CC
cord-009318-zt1o1bcz	46	26	held	hold	VBN
cord-009318-zt1o1bcz	46	27	for	for	IN
cord-009318-zt1o1bcz	46	28	30	30	CD
cord-009318-zt1o1bcz	46	29	s	s	NN
cord-009318-zt1o1bcz	46	30	to	to	TO
cord-009318-zt1o1bcz	46	31	inactivate	inactivate	VB
cord-009318-zt1o1bcz	46	32	the	the	DT
cord-009318-zt1o1bcz	46	33	enzymes	enzyme	NNS
cord-009318-zt1o1bcz	46	34	.	.	.
cord-009318-zt1o1bcz	47	1	Chips	chip	NNS
cord-009318-zt1o1bcz	47	2	were	be	VBD
cord-009318-zt1o1bcz	47	3	cooling	cool	VBG
cord-009318-zt1o1bcz	47	4	to	to	IN
cord-009318-zt1o1bcz	47	5	55	55	CD
cord-009318-zt1o1bcz	47	6	•	•	NNS
cord-009318-zt1o1bcz	47	7	C	c	NN
cord-009318-zt1o1bcz	47	8	and	and	CC
cord-009318-zt1o1bcz	47	9	the	the	DT
cord-009318-zt1o1bcz	47	10	melt	melt	NN
cord-009318-zt1o1bcz	47	11	performed	perform	VBN
cord-009318-zt1o1bcz	47	12	at	at	IN
cord-009318-zt1o1bcz	47	13	a	a	DT
cord-009318-zt1o1bcz	47	14	ramp	ramp	NN
cord-009318-zt1o1bcz	47	15	rate	rate	NN
cord-009318-zt1o1bcz	47	16	of	of	IN
cord-009318-zt1o1bcz	47	17	1	1	CD
cord-009318-zt1o1bcz	47	18	•	•	NN
cord-009318-zt1o1bcz	47	19	C	c	NN
cord-009318-zt1o1bcz	47	20	per	per	IN
cord-009318-zt1o1bcz	47	21	image	image	NN
cord-009318-zt1o1bcz	47	22	from	from	IN
cord-009318-zt1o1bcz	47	23	55	55	CD
cord-009318-zt1o1bcz	47	24	-	-	SYM
cord-009318-zt1o1bcz	47	25	90	90	CD
cord-009318-zt1o1bcz	47	26	•	•	NN
cord-009318-zt1o1bcz	47	27	C	C	NNP
cord-009318-zt1o1bcz	47	28	,	,	,
cord-009318-zt1o1bcz	47	29	and	and	CC
cord-009318-zt1o1bcz	47	30	a	a	DT
cord-009318-zt1o1bcz	47	31	ramp	ramp	NN
cord-009318-zt1o1bcz	47	32	rate	rate	NN
cord-009318-zt1o1bcz	47	33	of	of	IN
cord-009318-zt1o1bcz	47	34	0.5	0.5	CD
cord-009318-zt1o1bcz	47	35	•	•	NNS
cord-009318-zt1o1bcz	48	1	C	c	NN
cord-009318-zt1o1bcz	48	2	per	per	IN
cord-009318-zt1o1bcz	48	3	image	image	NN
cord-009318-zt1o1bcz	48	4	from	from	IN
cord-009318-zt1o1bcz	48	5	90	90	CD
cord-009318-zt1o1bcz	48	6	-	-	SYM
cord-009318-zt1o1bcz	48	7	95	95	CD
cord-009318-zt1o1bcz	48	8	•	•	NNS
cord-009318-zt1o1bcz	49	1	C.	C.	NNP
cord-009318-zt1o1bcz	49	2	A	a	DT
cord-009318-zt1o1bcz	49	3	frozen	frozen	JJ
cord-009318-zt1o1bcz	49	4	stock	stock	NN
cord-009318-zt1o1bcz	49	5	of	of	IN
cord-009318-zt1o1bcz	49	6	live	live	JJ
cord-009318-zt1o1bcz	49	7	CT	CT	NNP
cord-009318-zt1o1bcz	49	8	(	(	-LRB-
cord-009318-zt1o1bcz	49	9	D	D	NNP
cord-009318-zt1o1bcz	49	10	-	-	HYPH
cord-009318-zt1o1bcz	49	11	UW3	uw3	JJ
cord-009318-zt1o1bcz	49	12	,	,	,
cord-009318-zt1o1bcz	49	13	Z054	Z054	NNP
cord-009318-zt1o1bcz	49	14	,	,	,
cord-009318-zt1o1bcz	49	15	Zeptometrix	Zeptometrix	NNP
cord-009318-zt1o1bcz	49	16	,	,	,
cord-009318-zt1o1bcz	49	17	Buffalo	Buffalo	NNP
cord-009318-zt1o1bcz	49	18	,	,	,
cord-009318-zt1o1bcz	49	19	NY	NY	NNP
cord-009318-zt1o1bcz	49	20	,	,	,
cord-009318-zt1o1bcz	49	21	USA	USA	NNP
cord-009318-zt1o1bcz	49	22	)	)	-RRB-
cord-009318-zt1o1bcz	49	23	was	be	VBD
cord-009318-zt1o1bcz	49	24	re	re	VBN
cord-009318-zt1o1bcz	49	25	-	-	VBN
cord-009318-zt1o1bcz	49	26	suspended	suspend	VBN
cord-009318-zt1o1bcz	50	1	in	in	IN
cord-009318-zt1o1bcz	50	2	pre	pre	JJ
cord-009318-zt1o1bcz	50	3	-	-	JJ
cord-009318-zt1o1bcz	50	4	warmed	warm	VBN
cord-009318-zt1o1bcz	50	5	(	(	-LRB-
cord-009318-zt1o1bcz	50	6	37	37	CD
cord-009318-zt1o1bcz	50	7	•	•	NN
cord-009318-zt1o1bcz	50	8	C	c	NN
cord-009318-zt1o1bcz	50	9	)	)	-RRB-
cord-009318-zt1o1bcz	50	10	SPG	SPG	NNP
cord-009318-zt1o1bcz	50	11	buffer	buffer	NN
cord-009318-zt1o1bcz	50	12	(	(	-LRB-
cord-009318-zt1o1bcz	50	13	219	219	CD
cord-009318-zt1o1bcz	50	14	mM	mm	CD
cord-009318-zt1o1bcz	50	15	sucrose	sucrose	NN
cord-009318-zt1o1bcz	50	16	,	,	,
cord-009318-zt1o1bcz	50	17	3.7	3.7	CD
cord-009318-zt1o1bcz	50	18	mM	mm	CD
cord-009318-zt1o1bcz	50	19	KH2PO4	kh2po4	NN
cord-009318-zt1o1bcz	50	20	,	,	,
cord-009318-zt1o1bcz	50	21	8.5	8.5	CD
cord-009318-zt1o1bcz	50	22	mM	mm	CD
cord-009318-zt1o1bcz	50	23	NA2HPO4	na2hpo4	NN
cord-009318-zt1o1bcz	50	24	,	,	,
cord-009318-zt1o1bcz	50	25	and	and	CC
cord-009318-zt1o1bcz	50	26	4.9	4.9	CD
cord-009318-zt1o1bcz	50	27	mM	mm	CD
cord-009318-zt1o1bcz	50	28	L	l	NN
cord-009318-zt1o1bcz	50	29	-	-	HYPH
cord-009318-zt1o1bcz	50	30	glutamate	glutamate	NN
cord-009318-zt1o1bcz	50	31	)	)	-RRB-
cord-009318-zt1o1bcz	50	32	buffer	buffer	NN
cord-009318-zt1o1bcz	50	33	to	to	IN
cord-009318-zt1o1bcz	50	34	1	1	CD
cord-009318-zt1o1bcz	50	35	×	×	CD
cord-009318-zt1o1bcz	50	36	10	10	CD
cord-009318-zt1o1bcz	50	37	8	8	CD
cord-009318-zt1o1bcz	50	38	IFU	IFU	NNP
cord-009318-zt1o1bcz	50	39	/	/	SYM
cord-009318-zt1o1bcz	50	40	ml	ml	NN
cord-009318-zt1o1bcz	50	41	.	.	.
cord-009318-zt1o1bcz	51	1	It	-PRON-	PRP
cord-009318-zt1o1bcz	51	2	was	be	VBD
cord-009318-zt1o1bcz	51	3	then	then	RB
cord-009318-zt1o1bcz	51	4	diluted	dilute	VBN
cord-009318-zt1o1bcz	51	5	10-fold	10-fold	CD
cord-009318-zt1o1bcz	51	6	into	into	IN
cord-009318-zt1o1bcz	51	7	a	a	DT
cord-009318-zt1o1bcz	51	8	freshly	freshly	RB
cord-009318-zt1o1bcz	51	9	donated	donate	VBN
cord-009318-zt1o1bcz	51	10	urine	urine	NN
cord-009318-zt1o1bcz	51	11	sample	sample	NN
cord-009318-zt1o1bcz	51	12	to	to	IN
cord-009318-zt1o1bcz	51	13	1	1	CD
cord-009318-zt1o1bcz	51	14	×	×	CD
cord-009318-zt1o1bcz	51	15	10	10	CD
cord-009318-zt1o1bcz	51	16	7	7	CD
cord-009318-zt1o1bcz	51	17	IFU	IFU	NNP
cord-009318-zt1o1bcz	51	18	/	/	SYM
cord-009318-zt1o1bcz	51	19	ml	ml	NN
cord-009318-zt1o1bcz	51	20	.	.	.
cord-009318-zt1o1bcz	52	1	Urine	urine	NN
cord-009318-zt1o1bcz	52	2	from	from	IN
cord-009318-zt1o1bcz	52	3	a	a	DT
cord-009318-zt1o1bcz	52	4	healthy	healthy	JJ
cord-009318-zt1o1bcz	52	5	human	human	JJ
cord-009318-zt1o1bcz	52	6	donor	donor	NN
cord-009318-zt1o1bcz	52	7	(	(	-LRB-
cord-009318-zt1o1bcz	52	8	>	>	NFP
cord-009318-zt1o1bcz	52	9	18	18	CD
cord-009318-zt1o1bcz	52	10	years	year	NNS
cord-009318-zt1o1bcz	52	11	of	of	IN
cord-009318-zt1o1bcz	52	12	age	age	NN
cord-009318-zt1o1bcz	52	13	)	)	-RRB-
cord-009318-zt1o1bcz	52	14	was	be	VBD
cord-009318-zt1o1bcz	52	15	acquired	acquire	VBN
cord-009318-zt1o1bcz	52	16	and	and	CC
cord-009318-zt1o1bcz	52	17	used	use	VBN
cord-009318-zt1o1bcz	52	18	in	in	IN
cord-009318-zt1o1bcz	52	19	accordance	accordance	NN
cord-009318-zt1o1bcz	52	20	with	with	IN
cord-009318-zt1o1bcz	52	21	approved	approve	VBN
cord-009318-zt1o1bcz	52	22	Caltech	Caltech	NNP
cord-009318-zt1o1bcz	52	23	Institutional	Institutional	NNP
cord-009318-zt1o1bcz	52	24	Review	Review	NNP
cord-009318-zt1o1bcz	52	25	Board	Board	NNP
cord-009318-zt1o1bcz	52	26	(	(	-LRB-
cord-009318-zt1o1bcz	52	27	IRB	IRB	NNP
cord-009318-zt1o1bcz	52	28	)	)	-RRB-
cord-009318-zt1o1bcz	52	29	protocol	protocol	NN
cord-009318-zt1o1bcz	52	30	15	15	CD
cord-009318-zt1o1bcz	52	31	-	-	HYPH
cord-009318-zt1o1bcz	52	32	0566	0566	CD
cord-009318-zt1o1bcz	52	33	.	.	.
cord-009318-zt1o1bcz	53	1	Written	write	VBN
cord-009318-zt1o1bcz	53	2	informed	informed	JJ
cord-009318-zt1o1bcz	53	3	consent	consent	NN
cord-009318-zt1o1bcz	53	4	PAGE	page	NN
cord-009318-zt1o1bcz	53	5	3	3	CD
cord-009318-zt1o1bcz	53	6	OF	of	IN
cord-009318-zt1o1bcz	53	7	21	21	CD
cord-009318-zt1o1bcz	53	8	Nucleic	Nucleic	NNP
cord-009318-zt1o1bcz	53	9	Acids	Acids	NNPS
cord-009318-zt1o1bcz	53	10	Research	Research	NNP
cord-009318-zt1o1bcz	53	11	,	,	,
cord-009318-zt1o1bcz	53	12	2020	2020	CD
cord-009318-zt1o1bcz	53	13	,	,	,
cord-009318-zt1o1bcz	53	14	Vol	Vol	NNP
cord-009318-zt1o1bcz	53	15	.	.	.
cord-009318-zt1o1bcz	54	1	48	48	CD
cord-009318-zt1o1bcz	54	2	,	,	,
cord-009318-zt1o1bcz	54	3	No	no	NN
cord-009318-zt1o1bcz	54	4	.	.	NN
cord-009318-zt1o1bcz	54	5	7	7	CD
cord-009318-zt1o1bcz	54	6	e42	e42	NN
cord-009318-zt1o1bcz	54	7	was	be	VBD
cord-009318-zt1o1bcz	54	8	obtained	obtain	VBN
cord-009318-zt1o1bcz	54	9	from	from	IN
cord-009318-zt1o1bcz	54	10	all	all	DT
cord-009318-zt1o1bcz	54	11	participants	participant	NNS
cord-009318-zt1o1bcz	54	12	,	,	,
cord-009318-zt1o1bcz	54	13	donations	donation	NNS
cord-009318-zt1o1bcz	54	14	were	be	VBD
cord-009318-zt1o1bcz	54	15	never	never	RB
cord-009318-zt1o1bcz	54	16	tied	tie	VBN
cord-009318-zt1o1bcz	54	17	to	to	IN
cord-009318-zt1o1bcz	54	18	personal	personal	JJ
cord-009318-zt1o1bcz	54	19	identifiers	identifier	NNS
cord-009318-zt1o1bcz	54	20	and	and	CC
cord-009318-zt1o1bcz	54	21	all	all	DT
cord-009318-zt1o1bcz	54	22	research	research	NN
cord-009318-zt1o1bcz	54	23	was	be	VBD
cord-009318-zt1o1bcz	54	24	performed	perform	VBN
cord-009318-zt1o1bcz	54	25	in	in	IN
cord-009318-zt1o1bcz	54	26	accordance	accordance	NN
cord-009318-zt1o1bcz	54	27	with	with	IN
cord-009318-zt1o1bcz	54	28	relevant	relevant	JJ
cord-009318-zt1o1bcz	54	29	institutional	institutional	JJ
cord-009318-zt1o1bcz	54	30	biosafety	biosafety	NN
cord-009318-zt1o1bcz	54	31	regulations	regulation	NNS
cord-009318-zt1o1bcz	54	32	.	.	.
cord-009318-zt1o1bcz	55	1	A	a	DT
cord-009318-zt1o1bcz	55	2	250	250	CD
cord-009318-zt1o1bcz	55	3	l	l	NN
cord-009318-zt1o1bcz	55	4	aliquot	aliquot	NN
cord-009318-zt1o1bcz	55	5	from	from	IN
cord-009318-zt1o1bcz	55	6	this	this	DT
cord-009318-zt1o1bcz	55	7	CT	CT	NNP
cord-009318-zt1o1bcz	55	8	-	-	HYPH
cord-009318-zt1o1bcz	55	9	spiked	spike	VBN
cord-009318-zt1o1bcz	55	10	urine	urine	NN
cord-009318-zt1o1bcz	55	11	sample	sample	NN
cord-009318-zt1o1bcz	55	12	was	be	VBD
cord-009318-zt1o1bcz	55	13	then	then	RB
cord-009318-zt1o1bcz	55	14	extracted	extract	VBN
cord-009318-zt1o1bcz	55	15	following	follow	VBG
cord-009318-zt1o1bcz	55	16	the	the	DT
cord-009318-zt1o1bcz	55	17	ZR	ZR	NNP
cord-009318-zt1o1bcz	55	18	Viral	Viral	NNP
cord-009318-zt1o1bcz	55	19	DNA	DNA	NNP
cord-009318-zt1o1bcz	55	20	/	/	SYM
cord-009318-zt1o1bcz	55	21	RNA	RNA	NNP
cord-009318-zt1o1bcz	55	22	Kit	kit	NN
cord-009318-zt1o1bcz	55	23	protocol	protocol	NN
cord-009318-zt1o1bcz	55	24	(	(	-LRB-
cord-009318-zt1o1bcz	55	25	#	#	$
cord-009318-zt1o1bcz	55	26	D7020	d7020	NN
cord-009318-zt1o1bcz	55	27	,	,	,
cord-009318-zt1o1bcz	55	28	Zymo	Zymo	NNP
cord-009318-zt1o1bcz	55	29	Research	Research	NNP
cord-009318-zt1o1bcz	55	30	,	,	,
cord-009318-zt1o1bcz	55	31	Irvine	Irvine	NNP
cord-009318-zt1o1bcz	55	32	,	,	,
cord-009318-zt1o1bcz	55	33	CA	CA	NNP
cord-009318-zt1o1bcz	55	34	,	,	,
cord-009318-zt1o1bcz	55	35	USA	USA	NNP
cord-009318-zt1o1bcz	55	36	)	)	-RRB-
cord-009318-zt1o1bcz	55	37	.	.	.
cord-009318-zt1o1bcz	56	1	Briefly	briefly	RB
cord-009318-zt1o1bcz	56	2	,	,	,
cord-009318-zt1o1bcz	56	3	250	250	CD
cord-009318-zt1o1bcz	56	4	l	l	NN
cord-009318-zt1o1bcz	56	5	of	of	IN
cord-009318-zt1o1bcz	56	6	CT	CT	NNP
cord-009318-zt1o1bcz	56	7	-	-	HYPH
cord-009318-zt1o1bcz	56	8	spiked	spiked	JJ
cord-009318-zt1o1bcz	56	9	urine	urine	NN
cord-009318-zt1o1bcz	56	10	was	be	VBD
cord-009318-zt1o1bcz	56	11	mixed	mix	VBN
cord-009318-zt1o1bcz	56	12	with	with	IN
cord-009318-zt1o1bcz	56	13	250	250	CD
cord-009318-zt1o1bcz	56	14	l	l	NN
cord-009318-zt1o1bcz	56	15	DNA	DNA	NNP
cord-009318-zt1o1bcz	56	16	/	/	SYM
cord-009318-zt1o1bcz	56	17	RNA	RNA	NNP
cord-009318-zt1o1bcz	56	18	shield	shield	NN
cord-009318-zt1o1bcz	56	19	and	and	CC
cord-009318-zt1o1bcz	56	20	1000	1000	CD
cord-009318-zt1o1bcz	56	21	l	l	NN
cord-009318-zt1o1bcz	56	22	DNA	DNA	NNP
cord-009318-zt1o1bcz	56	23	/	/	SYM
cord-009318-zt1o1bcz	56	24	RNA	RNA	NNP
cord-009318-zt1o1bcz	56	25	Viral	Viral	NNP
cord-009318-zt1o1bcz	56	26	Buffer	Buffer	NNP
cord-009318-zt1o1bcz	56	27	.	.	.
cord-009318-zt1o1bcz	57	1	A	a	DT
cord-009318-zt1o1bcz	57	2	total	total	NN
cord-009318-zt1o1bcz	57	3	of	of	IN
cord-009318-zt1o1bcz	57	4	1500	1500	CD
cord-009318-zt1o1bcz	57	5	l	l	NN
cord-009318-zt1o1bcz	57	6	(	(	-LRB-
cord-009318-zt1o1bcz	57	7	750	750	CD
cord-009318-zt1o1bcz	57	8	l	l	NN
cord-009318-zt1o1bcz	57	9	×	×	NN
cord-009318-zt1o1bcz	57	10	2	2	CD
cord-009318-zt1o1bcz	57	11	)	)	-RRB-
cord-009318-zt1o1bcz	57	12	was	be	VBD
cord-009318-zt1o1bcz	57	13	added	add	VBN
cord-009318-zt1o1bcz	57	14	to	to	IN
cord-009318-zt1o1bcz	57	15	the	the	DT
cord-009318-zt1o1bcz	57	16	column	column	NN
cord-009318-zt1o1bcz	57	17	and	and	CC
cord-009318-zt1o1bcz	57	18	centrifuged	centrifuge	VBD
cord-009318-zt1o1bcz	57	19	at	at	IN
cord-009318-zt1o1bcz	57	20	16	16	CD
cord-009318-zt1o1bcz	57	21	000	000	CD
cord-009318-zt1o1bcz	57	22	×	×	NN
cord-009318-zt1o1bcz	57	23	g	g	NN
cord-009318-zt1o1bcz	57	24	for	for	IN
cord-009318-zt1o1bcz	57	25	1	1	CD
cord-009318-zt1o1bcz	57	26	min	min	NN
cord-009318-zt1o1bcz	57	27	.	.	.
cord-009318-zt1o1bcz	58	1	Then	then	RB
cord-009318-zt1o1bcz	58	2	,	,	,
cord-009318-zt1o1bcz	58	3	500	500	CD
cord-009318-zt1o1bcz	58	4	l	l	NN
cord-009318-zt1o1bcz	58	5	Viral	viral	JJ
cord-009318-zt1o1bcz	58	6	Wash	Wash	NNP
cord-009318-zt1o1bcz	58	7	buffer	buffer	NN
cord-009318-zt1o1bcz	58	8	was	be	VBD
cord-009318-zt1o1bcz	58	9	added	add	VBN
cord-009318-zt1o1bcz	58	10	to	to	IN
cord-009318-zt1o1bcz	58	11	the	the	DT
cord-009318-zt1o1bcz	58	12	column	column	NN
cord-009318-zt1o1bcz	58	13	and	and	CC
cord-009318-zt1o1bcz	58	14	centrifuged	centrifuge	VBD
cord-009318-zt1o1bcz	58	15	at	at	IN
cord-009318-zt1o1bcz	58	16	16	16	CD
cord-009318-zt1o1bcz	58	17	000	000	CD
cord-009318-zt1o1bcz	58	18	×	×	NN
cord-009318-zt1o1bcz	58	19	g	g	NN
cord-009318-zt1o1bcz	58	20	for	for	IN
cord-009318-zt1o1bcz	58	21	2	2	CD
cord-009318-zt1o1bcz	58	22	min	min	NN
cord-009318-zt1o1bcz	58	23	.	.	.
cord-009318-zt1o1bcz	59	1	Then	then	RB
cord-009318-zt1o1bcz	59	2	,	,	,
cord-009318-zt1o1bcz	59	3	60	60	CD
cord-009318-zt1o1bcz	59	4	l	l	NN
cord-009318-zt1o1bcz	59	5	DNAse	DNAse	NNP
cord-009318-zt1o1bcz	59	6	/	/	SYM
cord-009318-zt1o1bcz	59	7	RNAse	RNAse	NNP
cord-009318-zt1o1bcz	59	8	-	-	HYPH
cord-009318-zt1o1bcz	59	9	free	free	JJ
cord-009318-zt1o1bcz	59	10	water	water	NN
cord-009318-zt1o1bcz	59	11	was	be	VBD
cord-009318-zt1o1bcz	59	12	added	add	VBN
cord-009318-zt1o1bcz	59	13	directly	directly	RB
cord-009318-zt1o1bcz	59	14	to	to	IN
cord-009318-zt1o1bcz	59	15	the	the	DT
cord-009318-zt1o1bcz	59	16	column	column	NN
cord-009318-zt1o1bcz	59	17	and	and	CC
cord-009318-zt1o1bcz	59	18	centrifuged	centrifuge	VBD
cord-009318-zt1o1bcz	59	19	at	at	IN
cord-009318-zt1o1bcz	59	20	16	16	CD
cord-009318-zt1o1bcz	59	21	000	000	CD
cord-009318-zt1o1bcz	59	22	×	×	NN
cord-009318-zt1o1bcz	59	23	g	g	NN
cord-009318-zt1o1bcz	59	24	for	for	IN
cord-009318-zt1o1bcz	59	25	30	30	CD
cord-009318-zt1o1bcz	59	26	s.	s.	NN
cord-009318-zt1o1bcz	60	1	The	the	DT
cord-009318-zt1o1bcz	60	2	eluent	eluent	NN
cord-009318-zt1o1bcz	60	3	was	be	VBD
cord-009318-zt1o1bcz	60	4	treated	treat	VBN
cord-009318-zt1o1bcz	60	5	by	by	IN
cord-009318-zt1o1bcz	60	6	adding	add	VBG
cord-009318-zt1o1bcz	60	7	2.5	2.5	CD
cord-009318-zt1o1bcz	60	8	l	l	NNP
cord-009318-zt1o1bcz	60	9	Ambion	Ambion	NNP
cord-009318-zt1o1bcz	60	10	RNAse	RNAse	NNP
cord-009318-zt1o1bcz	60	11	Cocktail	cocktail	NN
cord-009318-zt1o1bcz	60	12	(	(	-LRB-
cord-009318-zt1o1bcz	60	13	#	#	VBG
cord-009318-zt1o1bcz	60	14	AM2286	am2286	XX
cord-009318-zt1o1bcz	60	15	,	,	,
cord-009318-zt1o1bcz	60	16	ThermoFisher	ThermoFisher	NNP
cord-009318-zt1o1bcz	60	17	)	)	-RRB-
cord-009318-zt1o1bcz	60	18	to	to	IN
cord-009318-zt1o1bcz	60	19	47.5	47.5	CD
cord-009318-zt1o1bcz	60	20	l	l	NN
cord-009318-zt1o1bcz	60	21	template	template	NN
cord-009318-zt1o1bcz	60	22	.	.	.
cord-009318-zt1o1bcz	61	1	Stocks	stock	NNS
cord-009318-zt1o1bcz	61	2	were	be	VBD
cord-009318-zt1o1bcz	61	3	prepared	prepare	VBN
cord-009318-zt1o1bcz	61	4	in	in	IN
cord-009318-zt1o1bcz	61	5	0.5	0.5	CD
cord-009318-zt1o1bcz	61	6	×	×	NNP
cord-009318-zt1o1bcz	61	7	TE	TE	NNP
cord-009318-zt1o1bcz	61	8	buffer	buffer	NN
cord-009318-zt1o1bcz	61	9	and	and	CC
cord-009318-zt1o1bcz	61	10	dilutions	dilution	NNS
cord-009318-zt1o1bcz	61	11	quantified	quantify	VBN
cord-009318-zt1o1bcz	61	12	using	use	VBG
cord-009318-zt1o1bcz	61	13	the	the	DT
cord-009318-zt1o1bcz	61	14	QX200	QX200	NNP
cord-009318-zt1o1bcz	61	15	droplet	droplet	NN
cord-009318-zt1o1bcz	61	16	digital	digital	JJ
cord-009318-zt1o1bcz	61	17	PCR	pcr	NN
cord-009318-zt1o1bcz	61	18	system	system	NN
cord-009318-zt1o1bcz	61	19	(	(	-LRB-
cord-009318-zt1o1bcz	61	20	Bio	Bio	NNP
cord-009318-zt1o1bcz	61	21	-	-	HYPH
cord-009318-zt1o1bcz	61	22	Rad	Rad	NNP
cord-009318-zt1o1bcz	61	23	Laboratories	Laboratories	NNP
cord-009318-zt1o1bcz	61	24	,	,	,
cord-009318-zt1o1bcz	61	25	Hercules	Hercules	NNP
cord-009318-zt1o1bcz	61	26	,	,	,
cord-009318-zt1o1bcz	61	27	CA	CA	NNP
cord-009318-zt1o1bcz	61	28	,	,	,
cord-009318-zt1o1bcz	61	29	USA	USA	NNP
cord-009318-zt1o1bcz	61	30	)	)	-RRB-
cord-009318-zt1o1bcz	61	31	,	,	,
cord-009318-zt1o1bcz	61	32	outer	outer	JJ
cord-009318-zt1o1bcz	61	33	primers	primer	NNS
cord-009318-zt1o1bcz	61	34	at	at	IN
cord-009318-zt1o1bcz	61	35	500	500	CD
cord-009318-zt1o1bcz	61	36	nM	nm	NN
cord-009318-zt1o1bcz	61	37	each	each	DT
cord-009318-zt1o1bcz	61	38	and	and	CC
cord-009318-zt1o1bcz	61	39	1×	1×	CD
cord-009318-zt1o1bcz	61	40	EvaGreen	EvaGreen	NNP
cord-009318-zt1o1bcz	61	41	Supermix	Supermix	NNP
cord-009318-zt1o1bcz	61	42	(	(	-LRB-
cord-009318-zt1o1bcz	61	43	Bio	Bio	NNP
cord-009318-zt1o1bcz	61	44	-	-	HYPH
cord-009318-zt1o1bcz	61	45	Rad	Rad	NNP
cord-009318-zt1o1bcz	61	46	)	)	-RRB-
cord-009318-zt1o1bcz	61	47	.	.	.
cord-009318-zt1o1bcz	62	1	A	a	DT
cord-009318-zt1o1bcz	62	2	Thermoelectric	Thermoelectric	NNP
cord-009318-zt1o1bcz	62	3	Module	module	NN
cord-009318-zt1o1bcz	62	4	(	(	-LRB-
cord-009318-zt1o1bcz	62	5	VT-127	VT-127	NNP
cord-009318-zt1o1bcz	62	6	-	-	HYPH
cord-009318-zt1o1bcz	62	7	1.4	1.4	CD
cord-009318-zt1o1bcz	62	8	-	-	HYPH
cord-009318-zt1o1bcz	62	9	1.5	1.5	CD
cord-009318-zt1o1bcz	62	10	-	-	SYM
cord-009318-zt1o1bcz	62	11	72	72	CD
cord-009318-zt1o1bcz	62	12	)	)	-RRB-
cord-009318-zt1o1bcz	62	13	,	,	,
cord-009318-zt1o1bcz	62	14	Thermister	Thermister	NNP
cord-009318-zt1o1bcz	62	15	(	(	-LRB-
cord-009318-zt1o1bcz	62	16	MP-3022	MP-3022	NNP
cord-009318-zt1o1bcz	62	17	)	)	-RRB-
cord-009318-zt1o1bcz	62	18	,	,	,
cord-009318-zt1o1bcz	62	19	Controller	Controller	NNP
cord-009318-zt1o1bcz	62	20	(	(	-LRB-
cord-009318-zt1o1bcz	62	21	TC-720	tc-720	NN
cord-009318-zt1o1bcz	62	22	)	)	-RRB-
cord-009318-zt1o1bcz	62	23	and	and	CC
cord-009318-zt1o1bcz	62	24	12V	12V	NNP
cord-009318-zt1o1bcz	62	25	Power	Power	NNP
cord-009318-zt1o1bcz	62	26	Supply	Supply	NNP
cord-009318-zt1o1bcz	62	27	(	(	-LRB-
cord-009318-zt1o1bcz	62	28	PS-12	PS-12	NNP
cord-009318-zt1o1bcz	62	29	-	-	HYPH
cord-009318-zt1o1bcz	62	30	8.4	8.4	CD
cord-009318-zt1o1bcz	62	31	;	;	:
cord-009318-zt1o1bcz	62	32	TE	TE	NNP
cord-009318-zt1o1bcz	62	33	Tech	Tech	NNP
cord-009318-zt1o1bcz	62	34	,	,	,
cord-009318-zt1o1bcz	62	35	Traverse	Traverse	NNP
cord-009318-zt1o1bcz	62	36	City	City	NNP
cord-009318-zt1o1bcz	62	37	,	,	,
cord-009318-zt1o1bcz	62	38	MI	MI	NNP
cord-009318-zt1o1bcz	62	39	,	,	,
cord-009318-zt1o1bcz	62	40	USA	USA	NNP
cord-009318-zt1o1bcz	62	41	)	)	-RRB-
cord-009318-zt1o1bcz	62	42	were	be	VBD
cord-009318-zt1o1bcz	62	43	wired	wire	VBN
cord-009318-zt1o1bcz	62	44	according	accord	VBG
cord-009318-zt1o1bcz	62	45	to	to	IN
cord-009318-zt1o1bcz	62	46	the	the	DT
cord-009318-zt1o1bcz	62	47	manufacturer	manufacturer	NN
cord-009318-zt1o1bcz	62	48	's	's	POS
cord-009318-zt1o1bcz	62	49	instructions	instruction	NNS
cord-009318-zt1o1bcz	62	50	.	.	.
cord-009318-zt1o1bcz	63	1	While	while	IN
cord-009318-zt1o1bcz	63	2	the	the	DT
cord-009318-zt1o1bcz	63	3	Peltier	Peltier	NNP
cord-009318-zt1o1bcz	63	4	can	can	MD
cord-009318-zt1o1bcz	63	5	be	be	VB
cord-009318-zt1o1bcz	63	6	used	use	VBN
cord-009318-zt1o1bcz	63	7	out	out	IN
cord-009318-zt1o1bcz	63	8	of	of	IN
cord-009318-zt1o1bcz	63	9	the	the	DT
cord-009318-zt1o1bcz	63	10	box	box	NN
cord-009318-zt1o1bcz	63	11	,	,	,
cord-009318-zt1o1bcz	63	12	we	-PRON-	PRP
cord-009318-zt1o1bcz	63	13	manufactured	manufacture	VBD
cord-009318-zt1o1bcz	63	14	a	a	DT
cord-009318-zt1o1bcz	63	15	heat	heat	NN
cord-009318-zt1o1bcz	63	16	plate	plate	NN
cord-009318-zt1o1bcz	63	17	and	and	CC
cord-009318-zt1o1bcz	63	18	sink	sink	NN
cord-009318-zt1o1bcz	63	19	to	to	TO
cord-009318-zt1o1bcz	63	20	improve	improve	VB
cord-009318-zt1o1bcz	63	21	the	the	DT
cord-009318-zt1o1bcz	63	22	efficiency	efficiency	NN
cord-009318-zt1o1bcz	63	23	in	in	IN
cord-009318-zt1o1bcz	63	24	the	the	DT
cord-009318-zt1o1bcz	63	25	cooling	cooling	NN
cord-009318-zt1o1bcz	63	26	mode	mode	NN
cord-009318-zt1o1bcz	63	27	.	.	.
cord-009318-zt1o1bcz	64	1	Instructions	instruction	NNS
cord-009318-zt1o1bcz	64	2	for	for	IN
cord-009318-zt1o1bcz	64	3	fabrication	fabrication	NN
cord-009318-zt1o1bcz	64	4	can	can	MD
cord-009318-zt1o1bcz	64	5	be	be	VB
cord-009318-zt1o1bcz	64	6	found	find	VBN
cord-009318-zt1o1bcz	64	7	in	in	IN
cord-009318-zt1o1bcz	64	8	the	the	DT
cord-009318-zt1o1bcz	64	9	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	64	10	Materials	Materials	NNPS
cord-009318-zt1o1bcz	64	11	and	and	CC
cord-009318-zt1o1bcz	64	12	Methods	Methods	NNPS
cord-009318-zt1o1bcz	64	13	,	,	,
cord-009318-zt1o1bcz	64	14	'	'	''
cord-009318-zt1o1bcz	64	15	Fabrication	Fabrication	NNP
cord-009318-zt1o1bcz	64	16	of	of	IN
cord-009318-zt1o1bcz	64	17	thermoelectric	thermoelectric	JJ
cord-009318-zt1o1bcz	64	18	unit	unit	NN
cord-009318-zt1o1bcz	64	19	mount	mount	NNP
cord-009318-zt1o1bcz	64	20	.	.	.
cord-009318-zt1o1bcz	64	21	'	'	''
cord-009318-zt1o1bcz	65	1	The	the	DT
cord-009318-zt1o1bcz	65	2	ability	ability	NN
cord-009318-zt1o1bcz	65	3	of	of	IN
cord-009318-zt1o1bcz	65	4	the	the	DT
cord-009318-zt1o1bcz	65	5	embedded	embed	VBN
cord-009318-zt1o1bcz	65	6	thermocouple	thermocouple	NN
cord-009318-zt1o1bcz	65	7	to	to	TO
cord-009318-zt1o1bcz	65	8	accurately	accurately	RB
cord-009318-zt1o1bcz	65	9	assess	assess	VB
cord-009318-zt1o1bcz	65	10	temperature	temperature	NN
cord-009318-zt1o1bcz	65	11	of	of	IN
cord-009318-zt1o1bcz	65	12	the	the	DT
cord-009318-zt1o1bcz	65	13	aluminum	aluminum	NN
cord-009318-zt1o1bcz	65	14	block	block	NN
cord-009318-zt1o1bcz	65	15	was	be	VBD
cord-009318-zt1o1bcz	65	16	verified	verify	VBN
cord-009318-zt1o1bcz	65	17	with	with	IN
cord-009318-zt1o1bcz	65	18	an	an	DT
cord-009318-zt1o1bcz	65	19	independent	independent	JJ
cord-009318-zt1o1bcz	65	20	K	k	NN
cord-009318-zt1o1bcz	65	21	-	-	HYPH
cord-009318-zt1o1bcz	65	22	type	type	NN
cord-009318-zt1o1bcz	65	23	minithermcouple	minithermcouple	NN
cord-009318-zt1o1bcz	65	24	read	read	VBD
cord-009318-zt1o1bcz	65	25	through	through	IN
cord-009318-zt1o1bcz	65	26	a	a	DT
cord-009318-zt1o1bcz	65	27	General	General	NNP
cord-009318-zt1o1bcz	65	28	IRT659	IRT659	NNP
cord-009318-zt1o1bcz	65	29	K	k	XX
cord-009318-zt1o1bcz	65	30	[	[	-LRB-
cord-009318-zt1o1bcz	65	31	IR	IR	NNP
cord-009318-zt1o1bcz	65	32	]	]	-RRB-
cord-009318-zt1o1bcz	65	33	Thermometer	Thermometer	NNP
cord-009318-zt1o1bcz	65	34	.	.	.
cord-009318-zt1o1bcz	66	1	Human	human	JJ
cord-009318-zt1o1bcz	66	2	genomic	genomic	JJ
cord-009318-zt1o1bcz	66	3	DNA	dna	NN
cord-009318-zt1o1bcz	66	4	from	from	IN
cord-009318-zt1o1bcz	66	5	buffy	buffy	NNP
cord-009318-zt1o1bcz	66	6	coat	coat	NNP
cord-009318-zt1o1bcz	66	7	leukocytes	leukocytes	NNP
cord-009318-zt1o1bcz	66	8	(	(	-LRB-
cord-009318-zt1o1bcz	66	9	Roche	Roche	NNP
cord-009318-zt1o1bcz	66	10	(	(	-LRB-
cord-009318-zt1o1bcz	66	11	via	via	IN
cord-009318-zt1o1bcz	66	12	Sigma	Sigma	NNP
cord-009318-zt1o1bcz	66	13	Aldrich	Aldrich	NNP
cord-009318-zt1o1bcz	66	14	)	)	-RRB-
cord-009318-zt1o1bcz	66	15	,	,	,
cord-009318-zt1o1bcz	66	16	#	#	$
cord-009318-zt1o1bcz	66	17	11691112001	11691112001	CD
cord-009318-zt1o1bcz	66	18	)	)	-RRB-
cord-009318-zt1o1bcz	66	19	was	be	VBD
cord-009318-zt1o1bcz	66	20	fragmented	fragment	VBN
cord-009318-zt1o1bcz	66	21	using	use	VBG
cord-009318-zt1o1bcz	66	22	a	a	DT
cord-009318-zt1o1bcz	66	23	Covaris	Covaris	NNP
cord-009318-zt1o1bcz	66	24	Focused	Focused	NNP
cord-009318-zt1o1bcz	66	25	Ultrasonicator	Ultrasonicator	NNP
cord-009318-zt1o1bcz	66	26	M220	M220	.
cord-009318-zt1o1bcz	66	27	(	(	-LRB-
cord-009318-zt1o1bcz	66	28	Woburn	Woburn	NNP
cord-009318-zt1o1bcz	66	29	,	,	,
cord-009318-zt1o1bcz	66	30	MA	MA	NNP
cord-009318-zt1o1bcz	66	31	,	,	,
cord-009318-zt1o1bcz	66	32	USA	USA	NNP
cord-009318-zt1o1bcz	66	33	)	)	-RRB-
cord-009318-zt1o1bcz	66	34	equipped	equip	VBD
cord-009318-zt1o1bcz	66	35	with	with	IN
cord-009318-zt1o1bcz	66	36	130	130	CD
cord-009318-zt1o1bcz	66	37	L	L	NNP
cord-009318-zt1o1bcz	66	38	microTUBE	microtube	NN
cord-009318-zt1o1bcz	66	39	AFA	AFA	NNP
cord-009318-zt1o1bcz	66	40	Fiber	Fiber	NNP
cord-009318-zt1o1bcz	66	41	Snap	Snap	NNP
cord-009318-zt1o1bcz	66	42	-	-	HYPH
cord-009318-zt1o1bcz	66	43	Cap	cap	NN
cord-009318-zt1o1bcz	66	44	at	at	IN
cord-009318-zt1o1bcz	66	45	50W	50w	JJ
cord-009318-zt1o1bcz	66	46	peak	peak	NN
cord-009318-zt1o1bcz	66	47	power	power	NN
cord-009318-zt1o1bcz	66	48	,	,	,
cord-009318-zt1o1bcz	66	49	5	5	CD
cord-009318-zt1o1bcz	66	50	%	%	NN
cord-009318-zt1o1bcz	66	51	duty	duty	NN
cord-009318-zt1o1bcz	66	52	factor	factor	NN
cord-009318-zt1o1bcz	66	53	,	,	,
cord-009318-zt1o1bcz	66	54	200	200	CD
cord-009318-zt1o1bcz	66	55	cycles	cycle	NNS
cord-009318-zt1o1bcz	66	56	per	per	IN
cord-009318-zt1o1bcz	66	57	burst	burst	NN
cord-009318-zt1o1bcz	66	58	,	,	,
cord-009318-zt1o1bcz	66	59	for	for	IN
cord-009318-zt1o1bcz	66	60	80	80	CD
cord-009318-zt1o1bcz	66	61	s.	s.	NNP
cord-009318-zt1o1bcz	66	62	Fragment	Fragment	NNP
cord-009318-zt1o1bcz	66	63	concentration	concentration	NN
cord-009318-zt1o1bcz	66	64	was	be	VBD
cord-009318-zt1o1bcz	66	65	determined	determine	VBN
cord-009318-zt1o1bcz	66	66	using	use	VBG
cord-009318-zt1o1bcz	66	67	a	a	DT
cord-009318-zt1o1bcz	66	68	Qbit	Qbit	NNP
cord-009318-zt1o1bcz	66	69	3	3	CD
cord-009318-zt1o1bcz	66	70	Fluorimiter	Fluorimiter	NNP
cord-009318-zt1o1bcz	66	71	(	(	-LRB-
cord-009318-zt1o1bcz	66	72	ThermoFisher	ThermoFisher	NNP
cord-009318-zt1o1bcz	66	73	,	,	,
cord-009318-zt1o1bcz	66	74	#	#	NN
cord-009318-zt1o1bcz	66	75	Q33216	Q33216	NNP
cord-009318-zt1o1bcz	66	76	)	)	-RRB-
cord-009318-zt1o1bcz	66	77	with	with	IN
cord-009318-zt1o1bcz	66	78	dsDNA	dsdna	JJ
cord-009318-zt1o1bcz	66	79	HS	HS	NNP
cord-009318-zt1o1bcz	66	80	assay	assay	NN
cord-009318-zt1o1bcz	66	81	kit	kit	NN
cord-009318-zt1o1bcz	66	82	(	(	-LRB-
cord-009318-zt1o1bcz	66	83	ThermoFisher	ThermoFisher	NNP
cord-009318-zt1o1bcz	66	84	,	,	,
cord-009318-zt1o1bcz	66	85	#	#	NN
cord-009318-zt1o1bcz	66	86	Q32851	q32851	NN
cord-009318-zt1o1bcz	66	87	)	)	-RRB-
cord-009318-zt1o1bcz	66	88	and	and	CC
cord-009318-zt1o1bcz	66	89	mean	mean	VB
cord-009318-zt1o1bcz	66	90	fragment	fragment	NN
cord-009318-zt1o1bcz	66	91	size	size	NN
cord-009318-zt1o1bcz	66	92	determined	determine	VBN
cord-009318-zt1o1bcz	66	93	as	as	IN
cord-009318-zt1o1bcz	66	94	365	365	CD
cord-009318-zt1o1bcz	66	95	bp	bp	NNS
cord-009318-zt1o1bcz	66	96	using	use	VBG
cord-009318-zt1o1bcz	66	97	an	an	DT
cord-009318-zt1o1bcz	66	98	Agilent	Agilent	NNP
cord-009318-zt1o1bcz	66	99	4200	4200	CD
cord-009318-zt1o1bcz	66	100	TapeStation	TapeStation	NNP
cord-009318-zt1o1bcz	66	101	(	(	-LRB-
cord-009318-zt1o1bcz	66	102	#	#	$
cord-009318-zt1o1bcz	66	103	G2991AA	G2991AA	NNP
cord-009318-zt1o1bcz	66	104	,	,	,
cord-009318-zt1o1bcz	66	105	Agilent	Agilent	NNP
cord-009318-zt1o1bcz	66	106	,	,	,
cord-009318-zt1o1bcz	66	107	Santa	Santa	NNP
cord-009318-zt1o1bcz	66	108	Clara	Clara	NNP
cord-009318-zt1o1bcz	66	109	,	,	,
cord-009318-zt1o1bcz	66	110	CA	CA	NNP
cord-009318-zt1o1bcz	66	111	,	,	,
cord-009318-zt1o1bcz	66	112	USA	USA	NNP
cord-009318-zt1o1bcz	66	113	)	)	-RRB-
cord-009318-zt1o1bcz	66	114	and	and	CC
cord-009318-zt1o1bcz	66	115	High	high	JJ
cord-009318-zt1o1bcz	66	116	Sensitivity	Sensitivity	NNP
cord-009318-zt1o1bcz	66	117	D5000	D5000	NNP
cord-009318-zt1o1bcz	66	118	ScreenTape	ScreenTape	NNP
cord-009318-zt1o1bcz	66	119	(	(	-LRB-
cord-009318-zt1o1bcz	66	120	#	#	$
cord-009318-zt1o1bcz	66	121	5067	5067	CD
cord-009318-zt1o1bcz	66	122	-	-	HYPH
cord-009318-zt1o1bcz	66	123	5592	5592	CD
cord-009318-zt1o1bcz	66	124	)	)	-RRB-
cord-009318-zt1o1bcz	66	125	with	with	IN
cord-009318-zt1o1bcz	66	126	ladder	ladder	NN
cord-009318-zt1o1bcz	66	127	(	(	-LRB-
cord-009318-zt1o1bcz	66	128	#	#	$
cord-009318-zt1o1bcz	66	129	5190	5190	CD
cord-009318-zt1o1bcz	66	130	-	-	HYPH
cord-009318-zt1o1bcz	66	131	7747	7747	CD
cord-009318-zt1o1bcz	66	132	)	)	-RRB-
cord-009318-zt1o1bcz	66	133	,	,	,
cord-009318-zt1o1bcz	66	134	and	and	CC
cord-009318-zt1o1bcz	66	135	D100	D100	NNP
cord-009318-zt1o1bcz	66	136	ScreenTape	ScreenTape	NNP
cord-009318-zt1o1bcz	66	137	(	(	-LRB-
cord-009318-zt1o1bcz	66	138	#	#	$
cord-009318-zt1o1bcz	66	139	5067	5067	CD
cord-009318-zt1o1bcz	66	140	-	-	HYPH
cord-009318-zt1o1bcz	66	141	5584	5584	CD
cord-009318-zt1o1bcz	66	142	)	)	-RRB-
cord-009318-zt1o1bcz	66	143	with	with	IN
cord-009318-zt1o1bcz	66	144	High	high	JJ
cord-009318-zt1o1bcz	66	145	Sensitivity	Sensitivity	NNP
cord-009318-zt1o1bcz	66	146	D1000	D1000	NNP
cord-009318-zt1o1bcz	66	147	Reagents	reagent	NNS
cord-009318-zt1o1bcz	66	148	(	(	-LRB-
cord-009318-zt1o1bcz	66	149	#	#	$
cord-009318-zt1o1bcz	66	150	5067	5067	CD
cord-009318-zt1o1bcz	66	151	-	-	HYPH
cord-009318-zt1o1bcz	66	152	5585	5585	CD
cord-009318-zt1o1bcz	66	153	)	)	-RRB-
cord-009318-zt1o1bcz	66	154	.	.	.
cord-009318-zt1o1bcz	67	1	Dilutions	dilution	NNS
cord-009318-zt1o1bcz	67	2	were	be	VBD
cord-009318-zt1o1bcz	67	3	prepared	prepare	VBN
cord-009318-zt1o1bcz	67	4	with	with	IN
cord-009318-zt1o1bcz	67	5	a	a	DT
cord-009318-zt1o1bcz	67	6	final	final	JJ
cord-009318-zt1o1bcz	67	7	concentration	concentration	NN
cord-009318-zt1o1bcz	67	8	of	of	IN
cord-009318-zt1o1bcz	67	9	0.5×	0.5×	CD
cord-009318-zt1o1bcz	67	10	TE	TE	NNP
cord-009318-zt1o1bcz	67	11	buffer	buffer	NN
cord-009318-zt1o1bcz	67	12	.	.	.
cord-009318-zt1o1bcz	68	1	Microfluidic	Microfluidic	NNP
cord-009318-zt1o1bcz	68	2	chips	chip	NNS
cord-009318-zt1o1bcz	68	3	for	for	IN
cord-009318-zt1o1bcz	68	4	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	69	1	(	(	-LRB-
cord-009318-zt1o1bcz	69	2	#	#	$
cord-009318-zt1o1bcz	69	3	A26316	a26316	NN
cord-009318-zt1o1bcz	69	4	;	;	:
cord-009318-zt1o1bcz	69	5	Applied	Applied	NNP
cord-009318-zt1o1bcz	69	6	Biosystems	Biosystems	NNP
cord-009318-zt1o1bcz	69	7	,	,	,
cord-009318-zt1o1bcz	69	8	Foster	Foster	NNP
cord-009318-zt1o1bcz	69	9	City	City	NNP
cord-009318-zt1o1bcz	69	10	,	,	,
cord-009318-zt1o1bcz	69	11	CA	CA	NNP
cord-009318-zt1o1bcz	69	12	,	,	,
cord-009318-zt1o1bcz	69	13	USA	USA	NNP
cord-009318-zt1o1bcz	69	14	)	)	-RRB-
cord-009318-zt1o1bcz	69	15	were	be	VBD
cord-009318-zt1o1bcz	69	16	loaded	load	VBN
cord-009318-zt1o1bcz	69	17	as	as	IN
cord-009318-zt1o1bcz	69	18	we	-PRON-	PRP
cord-009318-zt1o1bcz	69	19	have	have	VBP
cord-009318-zt1o1bcz	69	20	described	describe	VBN
cord-009318-zt1o1bcz	69	21	previously	previously	RB
cord-009318-zt1o1bcz	69	22	(	(	-LRB-
cord-009318-zt1o1bcz	69	23	23	23	CD
cord-009318-zt1o1bcz	69	24	)	)	-RRB-
cord-009318-zt1o1bcz	69	25	at	at	IN
cord-009318-zt1o1bcz	69	26	a	a	DT
cord-009318-zt1o1bcz	69	27	concentration	concentration	NN
cord-009318-zt1o1bcz	69	28	where	where	WRB
cord-009318-zt1o1bcz	69	29	∼40	∼40	NN
cord-009318-zt1o1bcz	69	30	%	%	NN
cord-009318-zt1o1bcz	69	31	of	of	IN
cord-009318-zt1o1bcz	69	32	partitions	partition	NNS
cord-009318-zt1o1bcz	69	33	would	would	MD
cord-009318-zt1o1bcz	69	34	fluoresce	fluoresce	VB
cord-009318-zt1o1bcz	69	35	(	(	-LRB-
cord-009318-zt1o1bcz	69	36	corresponding	correspond	VBG
cord-009318-zt1o1bcz	69	37	to	to	IN
cord-009318-zt1o1bcz	69	38	the	the	DT
cord-009318-zt1o1bcz	69	39	Poisson	Poisson	NNP
cord-009318-zt1o1bcz	69	40	maximum	maximum	JJ
cord-009318-zt1o1bcz	69	41	single	single	JJ
cord-009318-zt1o1bcz	69	42	template	template	NN
cord-009318-zt1o1bcz	69	43	per	per	IN
cord-009318-zt1o1bcz	69	44	partition	partition	NN
cord-009318-zt1o1bcz	69	45	loading	loading	NN
cord-009318-zt1o1bcz	69	46	of	of	IN
cord-009318-zt1o1bcz	69	47	660	660	CD
cord-009318-zt1o1bcz	69	48	cp	cp	NN
cord-009318-zt1o1bcz	69	49	/	/	SYM
cord-009318-zt1o1bcz	69	50	l	l	NN
cord-009318-zt1o1bcz	69	51	)	)	-RRB-
cord-009318-zt1o1bcz	69	52	.	.	.
cord-009318-zt1o1bcz	70	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	70	2	estimated	estimate	VBD
cord-009318-zt1o1bcz	70	3	the	the	DT
cord-009318-zt1o1bcz	70	4	volume	volume	NN
cord-009318-zt1o1bcz	70	5	of	of	IN
cord-009318-zt1o1bcz	70	6	each	each	DT
cord-009318-zt1o1bcz	70	7	partition	partition	NN
cord-009318-zt1o1bcz	70	8	to	to	TO
cord-009318-zt1o1bcz	70	9	be	be	VB
cord-009318-zt1o1bcz	70	10	750	750	CD
cord-009318-zt1o1bcz	70	11	pl	pl	NN
cord-009318-zt1o1bcz	70	12	.	.	.
cord-009318-zt1o1bcz	71	1	To	to	TO
cord-009318-zt1o1bcz	71	2	achieve	achieve	VB
cord-009318-zt1o1bcz	71	3	this	this	DT
cord-009318-zt1o1bcz	71	4	concentration	concentration	NN
cord-009318-zt1o1bcz	71	5	of	of	IN
cord-009318-zt1o1bcz	71	6	template	template	NN
cord-009318-zt1o1bcz	71	7	molecules	molecule	NNS
cord-009318-zt1o1bcz	71	8	,	,	,
cord-009318-zt1o1bcz	71	9	we	-PRON-	PRP
cord-009318-zt1o1bcz	71	10	diluted	dilute	VBD
cord-009318-zt1o1bcz	71	11	template	template	NN
cord-009318-zt1o1bcz	71	12	stocks	stock	NNS
cord-009318-zt1o1bcz	71	13	from	from	IN
cord-009318-zt1o1bcz	71	14	storage	storage	NN
cord-009318-zt1o1bcz	71	15	in	in	IN
cord-009318-zt1o1bcz	71	16	0.5×	0.5×	CD
cord-009318-zt1o1bcz	72	1	TE	TE	NNP
cord-009318-zt1o1bcz	72	2	to	to	IN
cord-009318-zt1o1bcz	72	3	∼0.03×	∼0.03×	NNP
cord-009318-zt1o1bcz	72	4	TE	TE	NNP
cord-009318-zt1o1bcz	72	5	for	for	IN
cord-009318-zt1o1bcz	72	6	all	all	DT
cord-009318-zt1o1bcz	72	7	experiments	experiment	NNS
cord-009318-zt1o1bcz	72	8	.	.	.
cord-009318-zt1o1bcz	73	1	Genomic	genomic	JJ
cord-009318-zt1o1bcz	73	2	DNA	dna	NN
cord-009318-zt1o1bcz	73	3	(	(	-LRB-
cord-009318-zt1o1bcz	73	4	gDNA	gDNA	NNS
cord-009318-zt1o1bcz	73	5	)	)	-RRB-
cord-009318-zt1o1bcz	73	6	stocks	stock	NNS
cord-009318-zt1o1bcz	73	7	,	,	,
cord-009318-zt1o1bcz	73	8	also	also	RB
cord-009318-zt1o1bcz	73	9	stored	store	VBN
cord-009318-zt1o1bcz	73	10	in	in	IN
cord-009318-zt1o1bcz	73	11	0.5×	0.5×	CD
cord-009318-zt1o1bcz	73	12	TE	TE	NNP
cord-009318-zt1o1bcz	73	13	,	,	,
cord-009318-zt1o1bcz	73	14	were	be	VBD
cord-009318-zt1o1bcz	73	15	diluted	dilute	VBN
cord-009318-zt1o1bcz	73	16	to	to	IN
cord-009318-zt1o1bcz	73	17	a	a	DT
cord-009318-zt1o1bcz	73	18	final	final	JJ
cord-009318-zt1o1bcz	73	19	concentration	concentration	NN
cord-009318-zt1o1bcz	73	20	of	of	IN
cord-009318-zt1o1bcz	73	21	0.077×.	0.077×.	NNP
cord-009318-zt1o1bcz	74	1	Thus	thus	RB
cord-009318-zt1o1bcz	74	2	,	,	,
cord-009318-zt1o1bcz	74	3	the	the	DT
cord-009318-zt1o1bcz	74	4	total	total	JJ
cord-009318-zt1o1bcz	74	5	final	final	JJ
cord-009318-zt1o1bcz	74	6	concentration	concentration	NN
cord-009318-zt1o1bcz	74	7	of	of	IN
cord-009318-zt1o1bcz	74	8	TE	TE	NNP
cord-009318-zt1o1bcz	74	9	for	for	IN
cord-009318-zt1o1bcz	74	10	all	all	DT
cord-009318-zt1o1bcz	74	11	experiments	experiment	NNS
cord-009318-zt1o1bcz	74	12	of	of	IN
cord-009318-zt1o1bcz	74	13	was	be	VBD
cord-009318-zt1o1bcz	74	14	∼0.1081×	∼0.1081×	IN
cord-009318-zt1o1bcz	74	15	TE	TE	NNP
cord-009318-zt1o1bcz	74	16	buffer	buffer	NN
cord-009318-zt1o1bcz	74	17	.	.	.
cord-009318-zt1o1bcz	75	1	Data	datum	NNS
cord-009318-zt1o1bcz	75	2	were	be	VBD
cord-009318-zt1o1bcz	75	3	collected	collect	VBN
cord-009318-zt1o1bcz	75	4	in	in	IN
cord-009318-zt1o1bcz	75	5	30-s	30-s	CD
cord-009318-zt1o1bcz	75	6	intervals	interval	NNS
cord-009318-zt1o1bcz	75	7	using	use	VBG
cord-009318-zt1o1bcz	75	8	a	a	DT
cord-009318-zt1o1bcz	75	9	DMI-6000B	dmi-6000b	NN
cord-009318-zt1o1bcz	75	10	microscope	microscope	NN
cord-009318-zt1o1bcz	75	11	(	(	-LRB-
cord-009318-zt1o1bcz	75	12	Leica	Leica	NNP
cord-009318-zt1o1bcz	75	13	,	,	,
cord-009318-zt1o1bcz	75	14	Buffalo	Buffalo	NNP
cord-009318-zt1o1bcz	75	15	Grove	Grove	NNP
cord-009318-zt1o1bcz	75	16	,	,	,
cord-009318-zt1o1bcz	75	17	IL	IL	NNP
cord-009318-zt1o1bcz	75	18	,	,	,
cord-009318-zt1o1bcz	75	19	USA	USA	NNP
cord-009318-zt1o1bcz	75	20	)	)	-RRB-
cord-009318-zt1o1bcz	75	21	equipped	equip	VBD
cord-009318-zt1o1bcz	75	22	with	with	IN
cord-009318-zt1o1bcz	75	23	a	a	DT
cord-009318-zt1o1bcz	75	24	1.25	1.25	CD
cord-009318-zt1o1bcz	75	25	×	×	NN
cord-009318-zt1o1bcz	75	26	0.04NA	0.04na	CD
cord-009318-zt1o1bcz	75	27	HCX	HCX	NNP
cord-009318-zt1o1bcz	75	28	PL	PL	NNP
cord-009318-zt1o1bcz	75	29	FLUOTAR	FLUOTAR	NNP
cord-009318-zt1o1bcz	75	30	Objective	Objective	NNP
cord-009318-zt1o1bcz	75	31	and	and	CC
cord-009318-zt1o1bcz	75	32	0.55×	0.55×	VBZ
cord-009318-zt1o1bcz	75	33	coupler	coupler	NN
cord-009318-zt1o1bcz	75	34	(	(	-LRB-
cord-009318-zt1o1bcz	75	35	Leica	Leica	NNP
cord-009318-zt1o1bcz	75	36	C	C	NNP
cord-009318-zt1o1bcz	75	37	-	-	HYPH
cord-009318-zt1o1bcz	75	38	mount	mount	NNP
cord-009318-zt1o1bcz	75	39	11541544	11541544	CD
cord-009318-zt1o1bcz	75	40	)	)	-RRB-
cord-009318-zt1o1bcz	75	41	.	.	.
cord-009318-zt1o1bcz	76	1	The	the	DT
cord-009318-zt1o1bcz	76	2	response	response	NN
cord-009318-zt1o1bcz	76	3	from	from	IN
cord-009318-zt1o1bcz	76	4	SYTO	SYTO	NNP
cord-009318-zt1o1bcz	76	5	9	9	CD
cord-009318-zt1o1bcz	76	6	was	be	VBD
cord-009318-zt1o1bcz	76	7	recorded	record	VBN
cord-009318-zt1o1bcz	76	8	using	use	VBG
cord-009318-zt1o1bcz	76	9	a	a	DT
cord-009318-zt1o1bcz	76	10	1.5-s	1.5-s	CD
cord-009318-zt1o1bcz	76	11	exposure	exposure	NN
cord-009318-zt1o1bcz	76	12	through	through	IN
cord-009318-zt1o1bcz	76	13	an	an	DT
cord-009318-zt1o1bcz	76	14	L5	l5	NN
cord-009318-zt1o1bcz	76	15	(	(	-LRB-
cord-009318-zt1o1bcz	76	16	GFP	GFP	NNP
cord-009318-zt1o1bcz	76	17	)	)	-RRB-
cord-009318-zt1o1bcz	76	18	Nomarski	Nomarski	NNP
cord-009318-zt1o1bcz	76	19	prism	prism	NN
cord-009318-zt1o1bcz	76	20	,	,	,
cord-009318-zt1o1bcz	76	21	while	while	IN
cord-009318-zt1o1bcz	76	22	the	the	DT
cord-009318-zt1o1bcz	76	23	ROX	ROX	NNP
cord-009318-zt1o1bcz	76	24	Reference	Reference	NNP
cord-009318-zt1o1bcz	76	25	Dye	Dye	NNP
cord-009318-zt1o1bcz	76	26	was	be	VBD
cord-009318-zt1o1bcz	76	27	collected	collect	VBN
cord-009318-zt1o1bcz	76	28	using	use	VBG
cord-009318-zt1o1bcz	76	29	a	a	DT
cord-009318-zt1o1bcz	76	30	1-s	1-s	CD
cord-009318-zt1o1bcz	76	31	exposure	exposure	NN
cord-009318-zt1o1bcz	76	32	through	through	IN
cord-009318-zt1o1bcz	76	33	a	a	DT
cord-009318-zt1o1bcz	76	34	Texas	Texas	NNP
cord-009318-zt1o1bcz	76	35	Red	Red	NNP
cord-009318-zt1o1bcz	76	36	prism	prism	NN
cord-009318-zt1o1bcz	76	37	.	.	.
cord-009318-zt1o1bcz	77	1	Images	image	NNS
cord-009318-zt1o1bcz	77	2	were	be	VBD
cord-009318-zt1o1bcz	77	3	collected	collect	VBN
cord-009318-zt1o1bcz	77	4	using	use	VBG
cord-009318-zt1o1bcz	77	5	a	a	DT
cord-009318-zt1o1bcz	77	6	Hamamatsu	Hamamatsu	NNP
cord-009318-zt1o1bcz	77	7	ORCA	ORCA	NNP
cord-009318-zt1o1bcz	77	8	-	-	HYPH
cord-009318-zt1o1bcz	77	9	ER	ER	NNP
cord-009318-zt1o1bcz	77	10	CCD	ccd	NN
cord-009318-zt1o1bcz	77	11	camera	camera	NN
cord-009318-zt1o1bcz	77	12	(	(	-LRB-
cord-009318-zt1o1bcz	77	13	Hamamatsu	Hamamatsu	NNP
cord-009318-zt1o1bcz	77	14	Photonics	Photonics	NNP
cord-009318-zt1o1bcz	77	15	K.K.	K.K.	NNP
cord-009318-zt1o1bcz	77	16	,	,	,
cord-009318-zt1o1bcz	77	17	Hamamatsu	Hamamatsu	NNP
cord-009318-zt1o1bcz	77	18	City	City	NNP
cord-009318-zt1o1bcz	77	19	,	,	,
cord-009318-zt1o1bcz	77	20	Japan	Japan	NNP
cord-009318-zt1o1bcz	77	21	)	)	-RRB-
cord-009318-zt1o1bcz	77	22	at	at	IN
cord-009318-zt1o1bcz	77	23	100	100	CD
cord-009318-zt1o1bcz	77	24	gain	gain	NN
cord-009318-zt1o1bcz	77	25	.	.	.
cord-009318-zt1o1bcz	78	1	Temperature	temperature	NN
cord-009318-zt1o1bcz	78	2	was	be	VBD
cord-009318-zt1o1bcz	78	3	recoded	recode	VBN
cord-009318-zt1o1bcz	78	4	using	use	VBG
cord-009318-zt1o1bcz	78	5	the	the	DT
cord-009318-zt1o1bcz	78	6	built	build	VBN
cord-009318-zt1o1bcz	78	7	-	-	HYPH
cord-009318-zt1o1bcz	78	8	in	in	RP
cord-009318-zt1o1bcz	78	9	features	feature	NNS
cord-009318-zt1o1bcz	78	10	of	of	IN
cord-009318-zt1o1bcz	78	11	the	the	DT
cord-009318-zt1o1bcz	78	12	TC-720	TC-720	NNP
cord-009318-zt1o1bcz	78	13	Controller	Controller	NNP
cord-009318-zt1o1bcz	78	14	in	in	IN
cord-009318-zt1o1bcz	78	15	∼1	∼1	NNP
cord-009318-zt1o1bcz	78	16	s	s	POS
cord-009318-zt1o1bcz	78	17	intervals	interval	NNS
cord-009318-zt1o1bcz	78	18	and	and	CC
cord-009318-zt1o1bcz	78	19	correlated	correlate	VBN
cord-009318-zt1o1bcz	78	20	to	to	IN
cord-009318-zt1o1bcz	78	21	the	the	DT
cord-009318-zt1o1bcz	78	22	images	image	NNS
cord-009318-zt1o1bcz	78	23	via	via	IN
cord-009318-zt1o1bcz	78	24	image	image	NN
cord-009318-zt1o1bcz	78	25	metadata	metadata	NN
cord-009318-zt1o1bcz	78	26	.	.	.
cord-009318-zt1o1bcz	79	1	In	in	IN
cord-009318-zt1o1bcz	79	2	these	these	DT
cord-009318-zt1o1bcz	79	3	experiments	experiment	NNS
cord-009318-zt1o1bcz	79	4	,	,	,
cord-009318-zt1o1bcz	79	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	79	6	chose	choose	VBD
cord-009318-zt1o1bcz	79	7	to	to	TO
cord-009318-zt1o1bcz	79	8	use	use	VB
cord-009318-zt1o1bcz	79	9	a	a	DT
cord-009318-zt1o1bcz	79	10	microscope	microscope	NN
cord-009318-zt1o1bcz	79	11	,	,	,
cord-009318-zt1o1bcz	79	12	instead	instead	RB
cord-009318-zt1o1bcz	79	13	of	of	IN
cord-009318-zt1o1bcz	79	14	the	the	DT
cord-009318-zt1o1bcz	79	15	custom	custom	JJ
cord-009318-zt1o1bcz	79	16	real	real	JJ
cord-009318-zt1o1bcz	79	17	-	-	HYPH
cord-009318-zt1o1bcz	79	18	time	time	NN
cord-009318-zt1o1bcz	79	19	amplification	amplification	NN
cord-009318-zt1o1bcz	79	20	instrument	instrument	NN
cord-009318-zt1o1bcz	79	21	we	-PRON-	PRP
cord-009318-zt1o1bcz	79	22	used	use	VBD
cord-009318-zt1o1bcz	79	23	previously	previously	RB
cord-009318-zt1o1bcz	79	24	(	(	-LRB-
cord-009318-zt1o1bcz	79	25	23	23	CD
cord-009318-zt1o1bcz	79	26	,	,	,
cord-009318-zt1o1bcz	79	27	24	24	CD
cord-009318-zt1o1bcz	79	28	)	)	-RRB-
cord-009318-zt1o1bcz	79	29	,	,	,
cord-009318-zt1o1bcz	79	30	because	because	IN
cord-009318-zt1o1bcz	79	31	the	the	DT
cord-009318-zt1o1bcz	79	32	microscope	microscope	NN
cord-009318-zt1o1bcz	79	33	has	have	VBZ
cord-009318-zt1o1bcz	79	34	superior	superior	JJ
cord-009318-zt1o1bcz	79	35	optical	optical	JJ
cord-009318-zt1o1bcz	79	36	properties	property	NNS
cord-009318-zt1o1bcz	79	37	(	(	-LRB-
cord-009318-zt1o1bcz	79	38	greater	great	JJR
cord-009318-zt1o1bcz	79	39	pixels	pixel	NNS
cord-009318-zt1o1bcz	79	40	per	per	IN
cord-009318-zt1o1bcz	79	41	partition	partition	NN
cord-009318-zt1o1bcz	79	42	and	and	CC
cord-009318-zt1o1bcz	79	43	lower	low	JJR
cord-009318-zt1o1bcz	79	44	exposure	exposure	NN
cord-009318-zt1o1bcz	79	45	time	time	NN
cord-009318-zt1o1bcz	79	46	requirements	requirement	NNS
cord-009318-zt1o1bcz	79	47	)	)	-RRB-
cord-009318-zt1o1bcz	79	48	to	to	TO
cord-009318-zt1o1bcz	79	49	access	access	VB
cord-009318-zt1o1bcz	79	50	higher	high	JJR
cord-009318-zt1o1bcz	79	51	temporal	temporal	JJ
cord-009318-zt1o1bcz	79	52	resolution	resolution	NN
cord-009318-zt1o1bcz	79	53	and	and	CC
cord-009318-zt1o1bcz	79	54	enhanced	enhance	VBN
cord-009318-zt1o1bcz	79	55	kinetic	kinetic	JJ
cord-009318-zt1o1bcz	79	56	measurements	measurement	NNS
cord-009318-zt1o1bcz	79	57	.	.	.
cord-009318-zt1o1bcz	80	1	The	the	DT
cord-009318-zt1o1bcz	80	2	MATLAB	MATLAB	NNP
cord-009318-zt1o1bcz	80	3	script	script	NN
cord-009318-zt1o1bcz	80	4	processes	process	VBZ
cord-009318-zt1o1bcz	80	5	a	a	DT
cord-009318-zt1o1bcz	80	6	.txt	.txt	NFP
cord-009318-zt1o1bcz	80	7	file	file	NN
cord-009318-zt1o1bcz	80	8	with	with	IN
cord-009318-zt1o1bcz	80	9	temperaturetime	temperaturetime	NNP
cord-009318-zt1o1bcz	80	10	data	datum	NNS
cord-009318-zt1o1bcz	80	11	generated	generate	VBN
cord-009318-zt1o1bcz	80	12	from	from	IN
cord-009318-zt1o1bcz	80	13	the	the	DT
cord-009318-zt1o1bcz	80	14	TE	TE	NNP
cord-009318-zt1o1bcz	80	15	Tech	Tech	NNP
cord-009318-zt1o1bcz	80	16	Controller	Controller	NNP
cord-009318-zt1o1bcz	80	17	and	and	CC
cord-009318-zt1o1bcz	80	18	a	a	DT
cord-009318-zt1o1bcz	80	19	TIF	tif	NN
cord-009318-zt1o1bcz	80	20	stack	stack	NN
cord-009318-zt1o1bcz	80	21	containing	contain	VBG
cord-009318-zt1o1bcz	80	22	2-channel	2-channel	JJ
cord-009318-zt1o1bcz	80	23	images	image	NNS
cord-009318-zt1o1bcz	80	24	of	of	IN
cord-009318-zt1o1bcz	80	25	the	the	DT
cord-009318-zt1o1bcz	80	26	LAMP	lamp	NN
cord-009318-zt1o1bcz	80	27	and	and	CC
cord-009318-zt1o1bcz	80	28	melt	melt	VB
cord-009318-zt1o1bcz	80	29	curve	curve	NN
cord-009318-zt1o1bcz	80	30	from	from	IN
cord-009318-zt1o1bcz	80	31	the	the	DT
cord-009318-zt1o1bcz	80	32	LEICA	LEICA	NNP
cord-009318-zt1o1bcz	80	33	microscope	microscope	NN
cord-009318-zt1o1bcz	80	34	.	.	.
cord-009318-zt1o1bcz	81	1	Partitions	partition	NNS
cord-009318-zt1o1bcz	81	2	are	be	VBP
cord-009318-zt1o1bcz	81	3	identified	identify	VBN
cord-009318-zt1o1bcz	81	4	using	use	VBG
cord-009318-zt1o1bcz	81	5	a	a	DT
cord-009318-zt1o1bcz	81	6	custom	custom	JJ
cord-009318-zt1o1bcz	81	7	iterative	iterative	JJ
cord-009318-zt1o1bcz	81	8	thresholding	thresholding	NN
cord-009318-zt1o1bcz	81	9	algorithm	algorithm	NN
cord-009318-zt1o1bcz	81	10	and	and	CC
cord-009318-zt1o1bcz	81	11	labels	label	NNS
cord-009318-zt1o1bcz	81	12	are	be	VBP
cord-009318-zt1o1bcz	81	13	propagated	propagate	VBN
cord-009318-zt1o1bcz	81	14	throughout	throughout	IN
cord-009318-zt1o1bcz	81	15	the	the	DT
cord-009318-zt1o1bcz	81	16	TIF	tif	NN
cord-009318-zt1o1bcz	81	17	stack	stack	NN
cord-009318-zt1o1bcz	81	18	using	use	VBG
cord-009318-zt1o1bcz	81	19	a	a	DT
cord-009318-zt1o1bcz	81	20	custom	custom	NN
cord-009318-zt1o1bcz	81	21	labeling	labeling	NN
cord-009318-zt1o1bcz	81	22	algorithm	algorithm	NN
cord-009318-zt1o1bcz	81	23	.	.	.
cord-009318-zt1o1bcz	82	1	Average	average	JJ
cord-009318-zt1o1bcz	82	2	well	well	JJ
cord-009318-zt1o1bcz	82	3	intensity	intensity	NN
cord-009318-zt1o1bcz	82	4	is	be	VBZ
cord-009318-zt1o1bcz	82	5	tracked	track	VBN
cord-009318-zt1o1bcz	82	6	over	over	IN
cord-009318-zt1o1bcz	82	7	time	time	NN
cord-009318-zt1o1bcz	82	8	to	to	TO
cord-009318-zt1o1bcz	82	9	generate	generate	VB
cord-009318-zt1o1bcz	82	10	LAMP	lamp	NN
cord-009318-zt1o1bcz	82	11	curves	curve	NNS
cord-009318-zt1o1bcz	82	12	and	and	CC
cord-009318-zt1o1bcz	82	13	plotted	plot	VBD
cord-009318-zt1o1bcz	82	14	against	against	IN
cord-009318-zt1o1bcz	82	15	temperature	temperature	NN
cord-009318-zt1o1bcz	82	16	to	to	TO
cord-009318-zt1o1bcz	82	17	generate	generate	VB
cord-009318-zt1o1bcz	82	18	the	the	DT
cord-009318-zt1o1bcz	82	19	melt	melt	NN
cord-009318-zt1o1bcz	82	20	curves	curve	NNS
cord-009318-zt1o1bcz	82	21	.	.	.
cord-009318-zt1o1bcz	83	1	Complete	complete	JJ
cord-009318-zt1o1bcz	83	2	details	detail	NNS
cord-009318-zt1o1bcz	83	3	of	of	IN
cord-009318-zt1o1bcz	83	4	the	the	DT
cord-009318-zt1o1bcz	83	5	script	script	NN
cord-009318-zt1o1bcz	83	6	are	be	VBP
cord-009318-zt1o1bcz	83	7	in	in	IN
cord-009318-zt1o1bcz	83	8	the	the	DT
cord-009318-zt1o1bcz	83	9	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	83	10	Materials	Materials	NNPS
cord-009318-zt1o1bcz	83	11	and	and	CC
cord-009318-zt1o1bcz	83	12	Methods	Methods	NNPS
cord-009318-zt1o1bcz	83	13	,	,	,
cord-009318-zt1o1bcz	83	14	'	'	''
cord-009318-zt1o1bcz	83	15	MAT	MAT	NNP
cord-009318-zt1o1bcz	83	16	-	-	HYPH
cord-009318-zt1o1bcz	83	17	LAB	LAB	NNP
cord-009318-zt1o1bcz	83	18	script	script	NN
cord-009318-zt1o1bcz	83	19	.	.	.
cord-009318-zt1o1bcz	83	20	'	'	''
cord-009318-zt1o1bcz	84	1	Bulk	Bulk	NNP
cord-009318-zt1o1bcz	84	2	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	84	3	reactions	reaction	NNS
cord-009318-zt1o1bcz	84	4	were	be	VBD
cord-009318-zt1o1bcz	84	5	conducted	conduct	VBN
cord-009318-zt1o1bcz	84	6	in	in	IN
cord-009318-zt1o1bcz	84	7	10	10	CD
cord-009318-zt1o1bcz	84	8	l	l	NN
cord-009318-zt1o1bcz	84	9	volumes	volume	NNS
cord-009318-zt1o1bcz	84	10	within	within	IN
cord-009318-zt1o1bcz	84	11	a	a	DT
cord-009318-zt1o1bcz	84	12	well	well	JJ
cord-009318-zt1o1bcz	84	13	plate	plate	NN
cord-009318-zt1o1bcz	84	14	on	on	IN
cord-009318-zt1o1bcz	84	15	a	a	DT
cord-009318-zt1o1bcz	84	16	CFX96	CFX96	NNP
cord-009318-zt1o1bcz	84	17	Real	real	JJ
cord-009318-zt1o1bcz	84	18	-	-	HYPH
cord-009318-zt1o1bcz	84	19	time	time	NN
cord-009318-zt1o1bcz	84	20	Thermocycler	Thermocycler	NNP
cord-009318-zt1o1bcz	84	21	(	(	-LRB-
cord-009318-zt1o1bcz	84	22	Bio	Bio	NNP
cord-009318-zt1o1bcz	84	23	-	-	HYPH
cord-009318-zt1o1bcz	84	24	Rad	Rad	NNP
cord-009318-zt1o1bcz	84	25	)	)	-RRB-
cord-009318-zt1o1bcz	84	26	at	at	IN
cord-009318-zt1o1bcz	84	27	buffer	buffer	NN
cord-009318-zt1o1bcz	84	28	conditions	condition	NNS
cord-009318-zt1o1bcz	84	29	and	and	CC
cord-009318-zt1o1bcz	84	30	temperatures	temperature	NNS
cord-009318-zt1o1bcz	84	31	matching	match	VBG
cord-009318-zt1o1bcz	84	32	the	the	DT
cord-009318-zt1o1bcz	84	33	dLAMP	dlamp	NN
cord-009318-zt1o1bcz	84	34	reactions	reaction	NNS
cord-009318-zt1o1bcz	84	35	.	.	.
cord-009318-zt1o1bcz	85	1	Enzymatic	enzymatic	JJ
cord-009318-zt1o1bcz	85	2	digestions	digestion	NNS
cord-009318-zt1o1bcz	85	3	of	of	IN
cord-009318-zt1o1bcz	85	4	bulk	bulk	JJ
cord-009318-zt1o1bcz	85	5	LAMP	lamp	NN
cord-009318-zt1o1bcz	85	6	products	product	NNS
cord-009318-zt1o1bcz	85	7	were	be	VBD
cord-009318-zt1o1bcz	85	8	conducted	conduct	VBN
cord-009318-zt1o1bcz	85	9	using	use	VBG
cord-009318-zt1o1bcz	85	10	CAC8I	cac8i	NN
cord-009318-zt1o1bcz	85	11	(	(	-LRB-
cord-009318-zt1o1bcz	85	12	#	#	$
cord-009318-zt1o1bcz	85	13	R0579S	R0579S	NNP
cord-009318-zt1o1bcz	85	14	)	)	-RRB-
cord-009318-zt1o1bcz	85	15	,	,	,
cord-009318-zt1o1bcz	85	16	Hpy166II	Hpy166II	NNP
cord-009318-zt1o1bcz	85	17	(	(	-LRB-
cord-009318-zt1o1bcz	85	18	#	#	$
cord-009318-zt1o1bcz	85	19	R0616S	r0616s	NN
cord-009318-zt1o1bcz	85	20	)	)	-RRB-
cord-009318-zt1o1bcz	85	21	,	,	,
cord-009318-zt1o1bcz	85	22	ACCI	ACCI	NNP
cord-009318-zt1o1bcz	85	23	(	(	-LRB-
cord-009318-zt1o1bcz	85	24	#	#	$
cord-009318-zt1o1bcz	85	25	R0161S	R0161S	NNP
cord-009318-zt1o1bcz	85	26	)	)	-RRB-
cord-009318-zt1o1bcz	85	27	,	,	,
cord-009318-zt1o1bcz	85	28	AciI	AciI	NNP
cord-009318-zt1o1bcz	85	29	(	(	-LRB-
cord-009318-zt1o1bcz	85	30	#	#	$
cord-009318-zt1o1bcz	85	31	SR0551S	sr0551s	CD
cord-009318-zt1o1bcz	85	32	)	)	-RRB-
cord-009318-zt1o1bcz	85	33	,	,	,
cord-009318-zt1o1bcz	85	34	MseI	MseI	NNP
cord-009318-zt1o1bcz	85	35	(	(	-LRB-
cord-009318-zt1o1bcz	85	36	#	#	$
cord-009318-zt1o1bcz	85	37	R0525S	R0525S	NNP
cord-009318-zt1o1bcz	85	38	)	)	-RRB-
cord-009318-zt1o1bcz	85	39	and	and	CC
cord-009318-zt1o1bcz	85	40	HpyCH4III	HpyCH4III	NNP
cord-009318-zt1o1bcz	85	41	(	(	-LRB-
cord-009318-zt1o1bcz	85	42	#	#	$
cord-009318-zt1o1bcz	85	43	R0618S	R0618S	NNP
cord-009318-zt1o1bcz	85	44	)	)	-RRB-
cord-009318-zt1o1bcz	85	45	purchased	purchase	VBD
cord-009318-zt1o1bcz	85	46	from	from	IN
cord-009318-zt1o1bcz	85	47	New	New	NNP
cord-009318-zt1o1bcz	85	48	England	England	NNP
cord-009318-zt1o1bcz	85	49	Biolabs	Biolabs	NNP
cord-009318-zt1o1bcz	85	50	and	and	CC
cord-009318-zt1o1bcz	85	51	were	be	VBD
cord-009318-zt1o1bcz	85	52	conducted	conduct	VBN
cord-009318-zt1o1bcz	85	53	in	in	IN
cord-009318-zt1o1bcz	85	54	50	50	CD
cord-009318-zt1o1bcz	85	55	l	l	NN
cord-009318-zt1o1bcz	85	56	reaction	reaction	NN
cord-009318-zt1o1bcz	85	57	volumes	volume	NNS
cord-009318-zt1o1bcz	85	58	containing	contain	VBG
cord-009318-zt1o1bcz	85	59	1	1	CD
cord-009318-zt1o1bcz	85	60	l	l	NN
cord-009318-zt1o1bcz	85	61	enzyme	enzyme	NN
cord-009318-zt1o1bcz	85	62	,	,	,
cord-009318-zt1o1bcz	85	63	1	1	CD
cord-009318-zt1o1bcz	85	64	g	g	NN
cord-009318-zt1o1bcz	85	65	DNA	dna	NN
cord-009318-zt1o1bcz	85	66	,	,	,
cord-009318-zt1o1bcz	85	67	in	in	IN
cord-009318-zt1o1bcz	85	68	1	1	CD
cord-009318-zt1o1bcz	85	69	×	×	NN
cord-009318-zt1o1bcz	85	70	Cut	Cut	VBN
cord-009318-zt1o1bcz	85	71	Smart	smart	JJ
cord-009318-zt1o1bcz	85	72	Buffer	Buffer	NNP
cord-009318-zt1o1bcz	85	73	and	and	CC
cord-009318-zt1o1bcz	85	74	incubated	incubate	VBN
cord-009318-zt1o1bcz	85	75	for	for	IN
cord-009318-zt1o1bcz	85	76	1	1	CD
cord-009318-zt1o1bcz	85	77	h	h	NN
cord-009318-zt1o1bcz	85	78	at	at	IN
cord-009318-zt1o1bcz	85	79	37	37	CD
cord-009318-zt1o1bcz	86	1	•	•	NNP
cord-009318-zt1o1bcz	87	1	C.	C.	NNP
cord-009318-zt1o1bcz	87	2	Samples	sample	NNS
cord-009318-zt1o1bcz	87	3	were	be	VBD
cord-009318-zt1o1bcz	87	4	inactivated	inactivate	VBN
cord-009318-zt1o1bcz	87	5	for	for	IN
cord-009318-zt1o1bcz	87	6	1	1	CD
cord-009318-zt1o1bcz	87	7	h	h	NN
cord-009318-zt1o1bcz	87	8	at	at	IN
cord-009318-zt1o1bcz	87	9	80	80	CD
cord-009318-zt1o1bcz	88	1	•	•	NNP
cord-009318-zt1o1bcz	88	2	C	C	NNP
cord-009318-zt1o1bcz	88	3	and	and	CC
cord-009318-zt1o1bcz	88	4	diluted	dilute	VBN
cord-009318-zt1o1bcz	88	5	to	to	IN
cord-009318-zt1o1bcz	88	6	1	1	CD
cord-009318-zt1o1bcz	88	7	ng	ng	NN
cord-009318-zt1o1bcz	88	8	/	/	SYM
cord-009318-zt1o1bcz	88	9	l	l	NN
cord-009318-zt1o1bcz	88	10	(	(	-LRB-
cord-009318-zt1o1bcz	88	11	∼1:300	∼1:300	NNP
cord-009318-zt1o1bcz	88	12	)	)	-RRB-
cord-009318-zt1o1bcz	89	1	to	to	TO
cord-009318-zt1o1bcz	89	2	run	run	VB
cord-009318-zt1o1bcz	89	3	on	on	IN
cord-009318-zt1o1bcz	89	4	an	an	DT
cord-009318-zt1o1bcz	89	5	Agilent	Agilent	NNP
cord-009318-zt1o1bcz	89	6	4200	4200	CD
cord-009318-zt1o1bcz	89	7	TapeStation	TapeStation	NNP
cord-009318-zt1o1bcz	89	8	using	use	VBG
cord-009318-zt1o1bcz	89	9	High	high	JJ
cord-009318-zt1o1bcz	89	10	Sensitivity	Sensitivity	NNP
cord-009318-zt1o1bcz	89	11	D5000	D5000	NNP
cord-009318-zt1o1bcz	89	12	ScreenTape	ScreenTape	NNP
cord-009318-zt1o1bcz	89	13	(	(	-LRB-
cord-009318-zt1o1bcz	89	14	#	#	$
cord-009318-zt1o1bcz	89	15	5067	5067	CD
cord-009318-zt1o1bcz	89	16	-	-	HYPH
cord-009318-zt1o1bcz	89	17	5592	5592	CD
cord-009318-zt1o1bcz	89	18	)	)	-RRB-
cord-009318-zt1o1bcz	89	19	with	with	IN
cord-009318-zt1o1bcz	89	20	ladder	ladder	NN
cord-009318-zt1o1bcz	89	21	(	(	-LRB-
cord-009318-zt1o1bcz	89	22	#	#	$
cord-009318-zt1o1bcz	89	23	5190	5190	CD
cord-009318-zt1o1bcz	89	24	-	-	HYPH
cord-009318-zt1o1bcz	89	25	7747	7747	CD
cord-009318-zt1o1bcz	89	26	)	)	-RRB-
cord-009318-zt1o1bcz	89	27	,	,	,
cord-009318-zt1o1bcz	89	28	and	and	CC
cord-009318-zt1o1bcz	89	29	D100	D100	NNP
cord-009318-zt1o1bcz	89	30	Screen	screen	NN
cord-009318-zt1o1bcz	89	31	-	-	HYPH
cord-009318-zt1o1bcz	89	32	Tape	Tape	NNP
cord-009318-zt1o1bcz	89	33	(	(	-LRB-
cord-009318-zt1o1bcz	89	34	#	#	$
cord-009318-zt1o1bcz	89	35	5067	5067	CD
cord-009318-zt1o1bcz	89	36	-	-	HYPH
cord-009318-zt1o1bcz	89	37	5584	5584	CD
cord-009318-zt1o1bcz	89	38	)	)	-RRB-
cord-009318-zt1o1bcz	89	39	with	with	IN
cord-009318-zt1o1bcz	89	40	High	high	JJ
cord-009318-zt1o1bcz	89	41	Sensitivity	Sensitivity	NNP
cord-009318-zt1o1bcz	89	42	D1000	D1000	NNP
cord-009318-zt1o1bcz	89	43	Reagents	reagent	NNS
cord-009318-zt1o1bcz	89	44	(	(	-LRB-
cord-009318-zt1o1bcz	89	45	#	#	$
cord-009318-zt1o1bcz	89	46	5067	5067	CD
cord-009318-zt1o1bcz	89	47	-	-	HYPH
cord-009318-zt1o1bcz	89	48	5585	5585	CD
cord-009318-zt1o1bcz	89	49	)	)	-RRB-
cord-009318-zt1o1bcz	89	50	.	.	.
cord-009318-zt1o1bcz	89	51	 	 	_SP
cord-009318-zt1o1bcz	90	1	The	the	DT
cord-009318-zt1o1bcz	90	2	300	300	CD
cord-009318-zt1o1bcz	90	3	-	-	SYM
cord-009318-zt1o1bcz	90	4	500	500	CD
cord-009318-zt1o1bcz	90	5	ng	ng	NN
cord-009318-zt1o1bcz	90	6	of	of	IN
cord-009318-zt1o1bcz	90	7	amplified	amplify	VBN
cord-009318-zt1o1bcz	90	8	DNA	dna	NN
cord-009318-zt1o1bcz	90	9	products	product	NNS
cord-009318-zt1o1bcz	90	10	were	be	VBD
cord-009318-zt1o1bcz	90	11	fragmented	fragmented	JJ
cord-009318-zt1o1bcz	90	12	to	to	IN
cord-009318-zt1o1bcz	90	13	the	the	DT
cord-009318-zt1o1bcz	90	14	average	average	JJ
cord-009318-zt1o1bcz	90	15	size	size	NN
cord-009318-zt1o1bcz	90	16	of	of	IN
cord-009318-zt1o1bcz	90	17	200	200	CD
cord-009318-zt1o1bcz	90	18	bp	bp	NNP
cord-009318-zt1o1bcz	90	19	with	with	IN
cord-009318-zt1o1bcz	90	20	Qsonica	Qsonica	NNP
cord-009318-zt1o1bcz	90	21	Q800R	Q800R	NNP
cord-009318-zt1o1bcz	90	22	sonicator	sonicator	NN
cord-009318-zt1o1bcz	90	23	(	(	-LRB-
cord-009318-zt1o1bcz	90	24	power	power	NN
cord-009318-zt1o1bcz	90	25	:	:	:
cord-009318-zt1o1bcz	90	26	20	20	CD
cord-009318-zt1o1bcz	90	27	%	%	NN
cord-009318-zt1o1bcz	90	28	;	;	.
cord-009318-zt1o1bcz	91	1	pulse	pulse	NN
cord-009318-zt1o1bcz	91	2	:	:	:
cord-009318-zt1o1bcz	92	1	15	15	CD
cord-009318-zt1o1bcz	92	2	s	s	NNPS
cord-009318-zt1o1bcz	92	3	on/15	on/15	NNP
cord-009318-zt1o1bcz	92	4	s	s	NN
cord-009318-zt1o1bcz	92	5	off	off	RP
cord-009318-zt1o1bcz	92	6	;	;	:
cord-009318-zt1o1bcz	92	7	sonication	sonication	NN
cord-009318-zt1o1bcz	92	8	time	time	NN
cord-009318-zt1o1bcz	92	9	:	:	:
cord-009318-zt1o1bcz	92	10	12	12	CD
cord-009318-zt1o1bcz	92	11	min	min	NN
cord-009318-zt1o1bcz	92	12	)	)	-RRB-
cord-009318-zt1o1bcz	92	13	and	and	CC
cord-009318-zt1o1bcz	92	14	libraries	library	NNS
cord-009318-zt1o1bcz	92	15	were	be	VBD
cord-009318-zt1o1bcz	92	16	constructed	construct	VBN
cord-009318-zt1o1bcz	92	17	using	use	VBG
cord-009318-zt1o1bcz	92	18	NEB	NEB	NNP
cord-009318-zt1o1bcz	92	19	-	-	HYPH
cord-009318-zt1o1bcz	92	20	Next	Next	NNP
cord-009318-zt1o1bcz	92	21	Ultra	Ultra	NNP
cord-009318-zt1o1bcz	92	22	™	™	NN
cord-009318-zt1o1bcz	92	23	II	II	NNP
cord-009318-zt1o1bcz	92	24	DNA	DNA	NNP
cord-009318-zt1o1bcz	92	25	Library	Library	NNP
cord-009318-zt1o1bcz	92	26	Prep	Prep	NNP
cord-009318-zt1o1bcz	92	27	Kit	Kit	NNP
cord-009318-zt1o1bcz	92	28	(	(	-LRB-
cord-009318-zt1o1bcz	92	29	NEB	NEB	NNP
cord-009318-zt1o1bcz	92	30	,	,	,
cord-009318-zt1o1bcz	92	31	#	#	$
cord-009318-zt1o1bcz	92	32	E7645	e7645	NN
cord-009318-zt1o1bcz	92	33	)	)	-RRB-
cord-009318-zt1o1bcz	92	34	following	follow	VBG
cord-009318-zt1o1bcz	92	35	manufacturer	manufacturer	NN
cord-009318-zt1o1bcz	92	36	's	's	POS
cord-009318-zt1o1bcz	92	37	instructions	instruction	NNS
cord-009318-zt1o1bcz	92	38	.	.	.
cord-009318-zt1o1bcz	93	1	Briefly	briefly	RB
cord-009318-zt1o1bcz	93	2	,	,	,
cord-009318-zt1o1bcz	93	3	fragmented	fragmented	JJ
cord-009318-zt1o1bcz	93	4	DNA	dna	NN
cord-009318-zt1o1bcz	93	5	was	be	VBD
cord-009318-zt1o1bcz	93	6	end	end	NN
cord-009318-zt1o1bcz	93	7	-	-	HYPH
cord-009318-zt1o1bcz	93	8	repaired	repair	VBN
cord-009318-zt1o1bcz	93	9	,	,	,
cord-009318-zt1o1bcz	93	10	dA	dA	NNP
cord-009318-zt1o1bcz	93	11	tailed	tailed	NNP
cord-009318-zt1o1bcz	93	12	and	and	CC
cord-009318-zt1o1bcz	93	13	ligated	ligate	VBD
cord-009318-zt1o1bcz	93	14	to	to	IN
cord-009318-zt1o1bcz	93	15	NEBNext	NEBNext	NNP
cord-009318-zt1o1bcz	93	16	hairpin	hairpin	NN
cord-009318-zt1o1bcz	93	17	adaptors	adaptor	NNS
cord-009318-zt1o1bcz	93	18	(	(	-LRB-
cord-009318-zt1o1bcz	93	19	NEB	NEB	NNP
cord-009318-zt1o1bcz	93	20	,	,	,
cord-009318-zt1o1bcz	93	21	#	#	NN
cord-009318-zt1o1bcz	93	22	E7335	e7335	NN
cord-009318-zt1o1bcz	93	23	)	)	-RRB-
cord-009318-zt1o1bcz	93	24	.	.	.
cord-009318-zt1o1bcz	94	1	After	after	IN
cord-009318-zt1o1bcz	94	2	ligation	ligation	NN
cord-009318-zt1o1bcz	94	3	,	,	,
cord-009318-zt1o1bcz	94	4	adapters	adapter	NNS
cord-009318-zt1o1bcz	94	5	were	be	VBD
cord-009318-zt1o1bcz	94	6	converted	convert	VBN
cord-009318-zt1o1bcz	94	7	to	to	IN
cord-009318-zt1o1bcz	94	8	the	the	DT
cord-009318-zt1o1bcz	94	9	'	'	``
cord-009318-zt1o1bcz	94	10	Y	Y	NNP
cord-009318-zt1o1bcz	94	11	'	'	''
cord-009318-zt1o1bcz	94	12	shape	shape	NN
cord-009318-zt1o1bcz	94	13	by	by	IN
cord-009318-zt1o1bcz	94	14	treating	treat	VBG
cord-009318-zt1o1bcz	94	15	with	with	IN
cord-009318-zt1o1bcz	94	16	USER	user	NN
cord-009318-zt1o1bcz	94	17	enzyme	enzyme	NN
cord-009318-zt1o1bcz	94	18	and	and	CC
cord-009318-zt1o1bcz	94	19	DNA	dna	NN
cord-009318-zt1o1bcz	94	20	fragments	fragment	NNS
cord-009318-zt1o1bcz	94	21	were	be	VBD
cord-009318-zt1o1bcz	94	22	size	size	NN
cord-009318-zt1o1bcz	94	23	selected	select	VBN
cord-009318-zt1o1bcz	94	24	using	use	VBG
cord-009318-zt1o1bcz	94	25	Agencourt	Agencourt	NNP
cord-009318-zt1o1bcz	94	26	AMPure	AMPure	NNP
cord-009318-zt1o1bcz	94	27	XP	XP	NNP
cord-009318-zt1o1bcz	94	28	beads	bead	NNS
cord-009318-zt1o1bcz	95	1	(	(	-LRB-
cord-009318-zt1o1bcz	95	2	Beckman	Beckman	NNP
cord-009318-zt1o1bcz	95	3	Coulter	Coulter	NNP
cord-009318-zt1o1bcz	95	4	,	,	,
cord-009318-zt1o1bcz	95	5	#	#	NN
cord-009318-zt1o1bcz	95	6	A63880	A63880	NNP
cord-009318-zt1o1bcz	95	7	)	)	-RRB-
cord-009318-zt1o1bcz	95	8	to	to	TO
cord-009318-zt1o1bcz	95	9	generate	generate	VB
cord-009318-zt1o1bcz	95	10	fragment	fragment	NN
cord-009318-zt1o1bcz	95	11	sizes	size	NNS
cord-009318-zt1o1bcz	95	12	between	between	IN
cord-009318-zt1o1bcz	95	13	250	250	CD
cord-009318-zt1o1bcz	95	14	and	and	CC
cord-009318-zt1o1bcz	95	15	350	350	CD
cord-009318-zt1o1bcz	95	16	bp	bp	NNS
cord-009318-zt1o1bcz	95	17	.	.	.
cord-009318-zt1o1bcz	96	1	Adaptorligated	Adaptorligated	NNP
cord-009318-zt1o1bcz	96	2	DNA	DNA	NNP
cord-009318-zt1o1bcz	96	3	was	be	VBD
cord-009318-zt1o1bcz	96	4	PCR	PCR	NNP
cord-009318-zt1o1bcz	96	5	amplified	amplify	VBN
cord-009318-zt1o1bcz	96	6	with	with	IN
cord-009318-zt1o1bcz	96	7	five	five	CD
cord-009318-zt1o1bcz	96	8	cycles	cycle	NNS
cord-009318-zt1o1bcz	96	9	followed	follow	VBN
cord-009318-zt1o1bcz	96	10	by	by	IN
cord-009318-zt1o1bcz	96	11	AMPure	AMPure	NNP
cord-009318-zt1o1bcz	96	12	XP	XP	NNP
cord-009318-zt1o1bcz	96	13	bead	bead	NN
cord-009318-zt1o1bcz	96	14	clean	clean	JJ
cord-009318-zt1o1bcz	96	15	up	up	RP
cord-009318-zt1o1bcz	96	16	.	.	.
cord-009318-zt1o1bcz	97	1	Libraries	library	NNS
cord-009318-zt1o1bcz	97	2	were	be	VBD
cord-009318-zt1o1bcz	97	3	quantified	quantify	VBN
cord-009318-zt1o1bcz	97	4	with	with	IN
cord-009318-zt1o1bcz	97	5	Qubit	Qubit	NNP
cord-009318-zt1o1bcz	97	6	dsDNA	dsdna	RB
cord-009318-zt1o1bcz	97	7	HS	HS	NNP
cord-009318-zt1o1bcz	97	8	Kit	Kit	NNP
cord-009318-zt1o1bcz	97	9	(	(	-LRB-
cord-009318-zt1o1bcz	97	10	ThermoFisher	ThermoFisher	NNP
cord-009318-zt1o1bcz	97	11	Scientific	Scientific	NNP
cord-009318-zt1o1bcz	97	12	,	,	,
cord-009318-zt1o1bcz	97	13	#	#	NN
cord-009318-zt1o1bcz	97	14	Q32854	Q32854	NNP
cord-009318-zt1o1bcz	97	15	)	)	-RRB-
cord-009318-zt1o1bcz	97	16	and	and	CC
cord-009318-zt1o1bcz	97	17	the	the	DT
cord-009318-zt1o1bcz	97	18	size	size	NN
cord-009318-zt1o1bcz	97	19	distribution	distribution	NN
cord-009318-zt1o1bcz	97	20	was	be	VBD
cord-009318-zt1o1bcz	97	21	confirmed	confirm	VBN
cord-009318-zt1o1bcz	97	22	with	with	IN
cord-009318-zt1o1bcz	97	23	High	high	JJ
cord-009318-zt1o1bcz	97	24	Sensitivity	Sensitivity	NNP
cord-009318-zt1o1bcz	97	25	DNA	dna	NN
cord-009318-zt1o1bcz	97	26	Kit	kit	NN
cord-009318-zt1o1bcz	97	27	for	for	IN
cord-009318-zt1o1bcz	97	28	Bioanalyzer	Bioanalyzer	NNP
cord-009318-zt1o1bcz	98	1	(	(	-LRB-
cord-009318-zt1o1bcz	98	2	Agilent	Agilent	NNP
cord-009318-zt1o1bcz	98	3	Technologies	Technologies	NNP
cord-009318-zt1o1bcz	98	4	,	,	,
cord-009318-zt1o1bcz	98	5	#	#	$
cord-009318-zt1o1bcz	98	6	5067	5067	CD
cord-009318-zt1o1bcz	98	7	)	)	-RRB-
cord-009318-zt1o1bcz	98	8	.	.	.
cord-009318-zt1o1bcz	99	1	Libraries	library	NNS
cord-009318-zt1o1bcz	99	2	were	be	VBD
cord-009318-zt1o1bcz	99	3	sequenced	sequence	VBN
cord-009318-zt1o1bcz	99	4	on	on	IN
cord-009318-zt1o1bcz	99	5	Illumina	Illumina	NNP
cord-009318-zt1o1bcz	99	6	HiSeq2500	HiSeq2500	NNP
cord-009318-zt1o1bcz	99	7	in	in	IN
cord-009318-zt1o1bcz	99	8	single	single	JJ
cord-009318-zt1o1bcz	99	9	-	-	HYPH
cord-009318-zt1o1bcz	99	10	read	read	NN
cord-009318-zt1o1bcz	99	11	mode	mode	NN
cord-009318-zt1o1bcz	99	12	with	with	IN
cord-009318-zt1o1bcz	99	13	the	the	DT
cord-009318-zt1o1bcz	99	14	read	read	VBN
cord-009318-zt1o1bcz	99	15	length	length	NN
cord-009318-zt1o1bcz	99	16	of	of	IN
cord-009318-zt1o1bcz	99	17	50	50	CD
cord-009318-zt1o1bcz	99	18	nt	nt	RB
cord-009318-zt1o1bcz	99	19	to	to	IN
cord-009318-zt1o1bcz	99	20	the	the	DT
cord-009318-zt1o1bcz	99	21	sequencing	sequencing	NN
cord-009318-zt1o1bcz	99	22	depth	depth	NN
cord-009318-zt1o1bcz	99	23	of	of	IN
cord-009318-zt1o1bcz	99	24	10	10	CD
cord-009318-zt1o1bcz	99	25	million	million	CD
cord-009318-zt1o1bcz	99	26	reads	read	NNS
cord-009318-zt1o1bcz	99	27	per	per	IN
cord-009318-zt1o1bcz	99	28	sample	sample	NN
cord-009318-zt1o1bcz	99	29	,	,	,
cord-009318-zt1o1bcz	99	30	following	follow	VBG
cord-009318-zt1o1bcz	99	31	manufacturer	manufacturer	NN
cord-009318-zt1o1bcz	99	32	's	's	POS
cord-009318-zt1o1bcz	99	33	instructions	instruction	NNS
cord-009318-zt1o1bcz	99	34	.	.	.
cord-009318-zt1o1bcz	100	1	Base	Base	NNP
cord-009318-zt1o1bcz	100	2	calls	call	NNS
cord-009318-zt1o1bcz	100	3	were	be	VBD
cord-009318-zt1o1bcz	100	4	performed	perform	VBN
cord-009318-zt1o1bcz	100	5	with	with	IN
cord-009318-zt1o1bcz	100	6	RTA	RTA	NNP
cord-009318-zt1o1bcz	100	7	1.18.64	1.18.64	CD
cord-009318-zt1o1bcz	100	8	followed	follow	VBD
cord-009318-zt1o1bcz	100	9	by	by	IN
cord-009318-zt1o1bcz	100	10	conversion	conversion	NN
cord-009318-zt1o1bcz	100	11	to	to	IN
cord-009318-zt1o1bcz	100	12	FASTQ	FASTQ	NNP
cord-009318-zt1o1bcz	100	13	with	with	IN
cord-009318-zt1o1bcz	100	14	bcl2fastq	bcl2fastq	NNP
cord-009318-zt1o1bcz	100	15	1.8.4	1.8.4	CD
cord-009318-zt1o1bcz	100	16	.	.	.
cord-009318-zt1o1bcz	101	1	Raw	Raw	NNP
cord-009318-zt1o1bcz	101	2	FASTQ	FASTQ	NNP
cord-009318-zt1o1bcz	101	3	files	file	NNS
cord-009318-zt1o1bcz	101	4	were	be	VBD
cord-009318-zt1o1bcz	101	5	first	first	RB
cord-009318-zt1o1bcz	101	6	analyzed	analyze	VBN
cord-009318-zt1o1bcz	101	7	with	with	IN
cord-009318-zt1o1bcz	101	8	FastQC	FastQC	NNP
cord-009318-zt1o1bcz	101	9	v0.11.8	v0.11.8	NNP
cord-009318-zt1o1bcz	101	10	.	.	.
cord-009318-zt1o1bcz	102	1	Overrepresented	overrepresented	JJ
cord-009318-zt1o1bcz	102	2	sequences	sequence	NNS
cord-009318-zt1o1bcz	102	3	were	be	VBD
cord-009318-zt1o1bcz	102	4	compared	compare	VBN
cord-009318-zt1o1bcz	102	5	with	with	IN
cord-009318-zt1o1bcz	102	6	input	input	NN
cord-009318-zt1o1bcz	102	7	primer	primer	NN
cord-009318-zt1o1bcz	102	8	sequences	sequence	NNS
cord-009318-zt1o1bcz	102	9	to	to	TO
cord-009318-zt1o1bcz	102	10	find	find	VB
cord-009318-zt1o1bcz	102	11	reads	read	NNS
cord-009318-zt1o1bcz	102	12	consisting	consist	VBG
cord-009318-zt1o1bcz	102	13	of	of	IN
cord-009318-zt1o1bcz	102	14	potential	potential	JJ
cord-009318-zt1o1bcz	102	15	products	product	NNS
cord-009318-zt1o1bcz	102	16	from	from	IN
cord-009318-zt1o1bcz	102	17	the	the	DT
cord-009318-zt1o1bcz	102	18	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	102	19	reactions	reaction	NNS
cord-009318-zt1o1bcz	102	20	.	.	.
cord-009318-zt1o1bcz	103	1	To	to	TO
cord-009318-zt1o1bcz	103	2	verify	verify	VB
cord-009318-zt1o1bcz	103	3	that	that	IN
cord-009318-zt1o1bcz	103	4	all	all	DT
cord-009318-zt1o1bcz	103	5	adjoining	adjoin	VBG
cord-009318-zt1o1bcz	103	6	products	product	NNS
cord-009318-zt1o1bcz	103	7	were	be	VBD
cord-009318-zt1o1bcz	103	8	accounted	account	VBN
cord-009318-zt1o1bcz	103	9	for	for	IN
cord-009318-zt1o1bcz	103	10	the	the	DT
cord-009318-zt1o1bcz	103	11	FASTQ	FASTQ	NNP
cord-009318-zt1o1bcz	103	12	files	file	NNS
cord-009318-zt1o1bcz	103	13	were	be	VBD
cord-009318-zt1o1bcz	103	14	aligned	align	VBN
cord-009318-zt1o1bcz	103	15	to	to	IN
cord-009318-zt1o1bcz	103	16	the	the	DT
cord-009318-zt1o1bcz	103	17	predicted	predict	VBN
cord-009318-zt1o1bcz	103	18	products	product	NNS
cord-009318-zt1o1bcz	103	19	using	use	VBG
cord-009318-zt1o1bcz	103	20	Bowtie2	Bowtie2	NNP
cord-009318-zt1o1bcz	103	21	v2.3.4.3	v2.3.4.3	NNS
cord-009318-zt1o1bcz	103	22	with	with	IN
cord-009318-zt1o1bcz	103	23	global	global	JJ
cord-009318-zt1o1bcz	103	24	very	very	RB
cord-009318-zt1o1bcz	103	25	-	-	HYPH
cord-009318-zt1o1bcz	103	26	sensitive	sensitive	JJ
cord-009318-zt1o1bcz	103	27	settings	setting	NNS
cord-009318-zt1o1bcz	103	28	.	.	.
cord-009318-zt1o1bcz	104	1	Unaligned	unaligned	JJ
cord-009318-zt1o1bcz	104	2	reads	read	NNS
cord-009318-zt1o1bcz	104	3	were	be	VBD
cord-009318-zt1o1bcz	104	4	checked	check	VBN
cord-009318-zt1o1bcz	104	5	for	for	IN
cord-009318-zt1o1bcz	104	6	any	any	DT
cord-009318-zt1o1bcz	104	7	remaining	remain	VBG
cord-009318-zt1o1bcz	104	8	possible	possible	JJ
cord-009318-zt1o1bcz	104	9	amplification	amplification	NN
cord-009318-zt1o1bcz	104	10	products	product	NNS
cord-009318-zt1o1bcz	104	11	.	.	.
cord-009318-zt1o1bcz	105	1	All	all	DT
cord-009318-zt1o1bcz	105	2	regions	region	NNS
cord-009318-zt1o1bcz	105	3	consisting	consist	VBG
cord-009318-zt1o1bcz	105	4	of	of	IN
cord-009318-zt1o1bcz	105	5	sequences	sequence	NNS
cord-009318-zt1o1bcz	105	6	from	from	IN
cord-009318-zt1o1bcz	105	7	multiple	multiple	JJ
cord-009318-zt1o1bcz	105	8	primers	primer	NNS
cord-009318-zt1o1bcz	105	9	were	be	VBD
cord-009318-zt1o1bcz	105	10	tallied	tally	VBN
cord-009318-zt1o1bcz	105	11	by	by	IN
cord-009318-zt1o1bcz	105	12	counting	count	VBG
cord-009318-zt1o1bcz	105	13	the	the	DT
cord-009318-zt1o1bcz	105	14	reads	read	NNS
cord-009318-zt1o1bcz	105	15	with	with	IN
cord-009318-zt1o1bcz	105	16	a	a	DT
cord-009318-zt1o1bcz	105	17	substring	substring	NN
cord-009318-zt1o1bcz	105	18	of	of	IN
cord-009318-zt1o1bcz	105	19	n	n	NN
cord-009318-zt1o1bcz	105	20	=	=	SYM
cord-009318-zt1o1bcz	105	21	11	11	CD
cord-009318-zt1o1bcz	105	22	from	from	IN
cord-009318-zt1o1bcz	105	23	the	the	DT
cord-009318-zt1o1bcz	105	24	end	end	NN
cord-009318-zt1o1bcz	105	25	of	of	IN
cord-009318-zt1o1bcz	105	26	each	each	DT
cord-009318-zt1o1bcz	105	27	primer	primer	NN
cord-009318-zt1o1bcz	105	28	.	.	.
cord-009318-zt1o1bcz	106	1	One	one	CD
cord-009318-zt1o1bcz	106	2	adjoining	adjoin	VBG
cord-009318-zt1o1bcz	106	3	region	region	NN
cord-009318-zt1o1bcz	106	4	between	between	IN
cord-009318-zt1o1bcz	106	5	primers	primer	NNS
cord-009318-zt1o1bcz	106	6	contained	contain	VBD
cord-009318-zt1o1bcz	106	7	a	a	DT
cord-009318-zt1o1bcz	106	8	random	random	JJ
cord-009318-zt1o1bcz	106	9	insertion	insertion	NN
cord-009318-zt1o1bcz	106	10	of	of	IN
cord-009318-zt1o1bcz	106	11	nucleotides	nucleotide	NNS
cord-009318-zt1o1bcz	106	12	and	and	CC
cord-009318-zt1o1bcz	106	13	was	be	VBD
cord-009318-zt1o1bcz	106	14	analyzed	analyze	VBN
cord-009318-zt1o1bcz	106	15	by	by	IN
cord-009318-zt1o1bcz	106	16	first	first	RB
cord-009318-zt1o1bcz	106	17	extracting	extract	VBG
cord-009318-zt1o1bcz	106	18	all	all	DT
cord-009318-zt1o1bcz	106	19	reads	read	NNS
cord-009318-zt1o1bcz	106	20	containing	contain	VBG
cord-009318-zt1o1bcz	106	21	the	the	DT
cord-009318-zt1o1bcz	106	22	primer	primer	NN
cord-009318-zt1o1bcz	106	23	before	before	RB
cord-009318-zt1o1bcz	106	24	and	and	CC
cord-009318-zt1o1bcz	106	25	after	after	IN
cord-009318-zt1o1bcz	106	26	the	the	DT
cord-009318-zt1o1bcz	106	27	random	random	JJ
cord-009318-zt1o1bcz	106	28	nucleotides	nucleotide	NNS
cord-009318-zt1o1bcz	106	29	.	.	.
cord-009318-zt1o1bcz	107	1	The	the	DT
cord-009318-zt1o1bcz	107	2	length	length	NN
cord-009318-zt1o1bcz	107	3	and	and	CC
cord-009318-zt1o1bcz	107	4	sequence	sequence	NN
cord-009318-zt1o1bcz	107	5	distribution	distribution	NN
cord-009318-zt1o1bcz	107	6	of	of	IN
cord-009318-zt1o1bcz	107	7	random	random	JJ
cord-009318-zt1o1bcz	107	8	inserts	insert	NNS
cord-009318-zt1o1bcz	107	9	was	be	VBD
cord-009318-zt1o1bcz	107	10	then	then	RB
cord-009318-zt1o1bcz	107	11	analyzed	analyze	VBN
cord-009318-zt1o1bcz	107	12	from	from	IN
cord-009318-zt1o1bcz	107	13	the	the	DT
cord-009318-zt1o1bcz	107	14	extracted	extract	VBN
cord-009318-zt1o1bcz	107	15	reads	read	NNS
cord-009318-zt1o1bcz	107	16	.	.	.
cord-009318-zt1o1bcz	108	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	108	2	first	first	RB
cord-009318-zt1o1bcz	108	3	wished	wish	VBD
cord-009318-zt1o1bcz	108	4	to	to	TO
cord-009318-zt1o1bcz	108	5	test	test	VB
cord-009318-zt1o1bcz	108	6	whether	whether	IN
cord-009318-zt1o1bcz	108	7	melting	melt	VBG
cord-009318-zt1o1bcz	108	8	temperature	temperature	NN
cord-009318-zt1o1bcz	108	9	(	(	-LRB-
cord-009318-zt1o1bcz	108	10	T	T	NNP
cord-009318-zt1o1bcz	108	11	m	m	NN
cord-009318-zt1o1bcz	108	12	)	)	-RRB-
cord-009318-zt1o1bcz	108	13	could	could	MD
cord-009318-zt1o1bcz	108	14	be	be	VB
cord-009318-zt1o1bcz	108	15	used	use	VBN
cord-009318-zt1o1bcz	108	16	to	to	TO
cord-009318-zt1o1bcz	108	17	separate	separate	VB
cord-009318-zt1o1bcz	108	18	specific	specific	JJ
cord-009318-zt1o1bcz	108	19	and	and	CC
cord-009318-zt1o1bcz	108	20	non	non	JJ
cord-009318-zt1o1bcz	108	21	-	-	JJ
cord-009318-zt1o1bcz	108	22	specific	specific	JJ
cord-009318-zt1o1bcz	108	23	amplification	amplification	NN
cord-009318-zt1o1bcz	108	24	in	in	IN
cord-009318-zt1o1bcz	108	25	a	a	DT
cord-009318-zt1o1bcz	108	26	LAMP	lamp	NN
cord-009318-zt1o1bcz	108	27	assay	assay	NN
cord-009318-zt1o1bcz	108	28	run	run	VBN
cord-009318-zt1o1bcz	108	29	in	in	IN
cord-009318-zt1o1bcz	108	30	bulk	bulk	NN
cord-009318-zt1o1bcz	108	31	.	.	.
cord-009318-zt1o1bcz	109	1	To	to	TO
cord-009318-zt1o1bcz	109	2	start	start	VB
cord-009318-zt1o1bcz	109	3	,	,	,
cord-009318-zt1o1bcz	109	4	we	-PRON-	PRP
cord-009318-zt1o1bcz	109	5	selected	select	VBD
cord-009318-zt1o1bcz	109	6	a	a	DT
cord-009318-zt1o1bcz	109	7	concentration	concentration	NN
cord-009318-zt1o1bcz	109	8	near	near	IN
cord-009318-zt1o1bcz	109	9	the	the	DT
cord-009318-zt1o1bcz	109	10	LOD	lod	NN
cord-009318-zt1o1bcz	109	11	where	where	WRB
cord-009318-zt1o1bcz	109	12	we	-PRON-	PRP
cord-009318-zt1o1bcz	109	13	might	may	MD
cord-009318-zt1o1bcz	109	14	observe	observe	VB
cord-009318-zt1o1bcz	109	15	both	both	DT
cord-009318-zt1o1bcz	109	16	specific	specific	JJ
cord-009318-zt1o1bcz	109	17	and	and	CC
cord-009318-zt1o1bcz	109	18	non	non	JJ
cord-009318-zt1o1bcz	109	19	-	-	JJ
cord-009318-zt1o1bcz	109	20	specific	specific	JJ
cord-009318-zt1o1bcz	109	21	amplification	amplification	NN
cord-009318-zt1o1bcz	109	22	.	.	.
cord-009318-zt1o1bcz	110	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	110	2	used	use	VBD
cord-009318-zt1o1bcz	110	3	extracted	extract	VBN
cord-009318-zt1o1bcz	110	4	CT	CT	NNP
cord-009318-zt1o1bcz	110	5	genomic	genomic	JJ
cord-009318-zt1o1bcz	110	6	DNA	dna	NN
cord-009318-zt1o1bcz	110	7	in	in	IN
cord-009318-zt1o1bcz	110	8	the	the	DT
cord-009318-zt1o1bcz	110	9	presence	presence	NN
cord-009318-zt1o1bcz	110	10	of	of	IN
cord-009318-zt1o1bcz	110	11	two	two	CD
cord-009318-zt1o1bcz	110	12	commercially	commercially	RB
cord-009318-zt1o1bcz	110	13	available	available	JJ
cord-009318-zt1o1bcz	110	14	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	110	15	,	,	,
cord-009318-zt1o1bcz	110	16	Bst	Bst	NNP
cord-009318-zt1o1bcz	110	17	2.0	2.0	NNP
cord-009318-zt1o1bcz	110	18	and	and	CC
cord-009318-zt1o1bcz	110	19	Bst	Bst	NNP
cord-009318-zt1o1bcz	110	20	3.0	3.0	NNP
cord-009318-zt1o1bcz	110	21	,	,	,
cord-009318-zt1o1bcz	110	22	with	with	IN
cord-009318-zt1o1bcz	110	23	CT	CT	NNP
cord-009318-zt1o1bcz	110	24	23S	23S	NNP
cord-009318-zt1o1bcz	110	25	as	as	IN
cord-009318-zt1o1bcz	110	26	the	the	DT
cord-009318-zt1o1bcz	110	27	amplification	amplification	NN
cord-009318-zt1o1bcz	110	28	target	target	NN
cord-009318-zt1o1bcz	110	29	.	.	.
cord-009318-zt1o1bcz	111	1	At	at	IN
cord-009318-zt1o1bcz	111	2	target	target	NN
cord-009318-zt1o1bcz	111	3	molecule	molecule	NN
cord-009318-zt1o1bcz	111	4	concentrations	concentration	NNS
cord-009318-zt1o1bcz	111	5	of	of	IN
cord-009318-zt1o1bcz	111	6	10	10	CD
cord-009318-zt1o1bcz	111	7	copies	copy	NNS
cord-009318-zt1o1bcz	111	8	per	per	IN
cord-009318-zt1o1bcz	111	9	L	l	NN
cord-009318-zt1o1bcz	111	10	(	(	-LRB-
cord-009318-zt1o1bcz	111	11	cp	cp	NNP
cord-009318-zt1o1bcz	111	12	/	/	SYM
cord-009318-zt1o1bcz	111	13	l	l	NN
cord-009318-zt1o1bcz	111	14	)	)	-RRB-
cord-009318-zt1o1bcz	111	15	,	,	,
cord-009318-zt1o1bcz	111	16	amplification	amplification	NN
cord-009318-zt1o1bcz	111	17	using	use	VBG
cord-009318-zt1o1bcz	111	18	Bst	Bst	NNP
cord-009318-zt1o1bcz	111	19	2.0	2.0	CD
cord-009318-zt1o1bcz	111	20	polymerase	polymerase	NN
cord-009318-zt1o1bcz	111	21	began	begin	VBD
cord-009318-zt1o1bcz	111	22	between	between	IN
cord-009318-zt1o1bcz	111	23	10	10	CD
cord-009318-zt1o1bcz	111	24	-	-	SYM
cord-009318-zt1o1bcz	111	25	11	11	CD
cord-009318-zt1o1bcz	111	26	min	min	NN
cord-009318-zt1o1bcz	111	27	(	(	-LRB-
cord-009318-zt1o1bcz	111	28	Figure	figure	NN
cord-009318-zt1o1bcz	111	29	1A	1a	NN
cord-009318-zt1o1bcz	111	30	)	)	-RRB-
cord-009318-zt1o1bcz	111	31	and	and	CC
cord-009318-zt1o1bcz	111	32	had	have	VBD
cord-009318-zt1o1bcz	111	33	uniform	uniform	JJ
cord-009318-zt1o1bcz	111	34	T	T	NNP
cord-009318-zt1o1bcz	111	35	m	m	NN
cord-009318-zt1o1bcz	111	36	(	(	-LRB-
cord-009318-zt1o1bcz	111	37	Figure	figure	NN
cord-009318-zt1o1bcz	111	38	1B	1b	CD
cord-009318-zt1o1bcz	111	39	)	)	-RRB-
cord-009318-zt1o1bcz	111	40	.	.	.
cord-009318-zt1o1bcz	112	1	Amplification	amplification	NN
cord-009318-zt1o1bcz	112	2	using	use	VBG
cord-009318-zt1o1bcz	112	3	Bst	Bst	NNP
cord-009318-zt1o1bcz	112	4	3.0	3.0	CD
cord-009318-zt1o1bcz	112	5	polymerase	polymerase	NN
cord-009318-zt1o1bcz	112	6	(	(	-LRB-
cord-009318-zt1o1bcz	112	7	Figure	figure	NN
cord-009318-zt1o1bcz	112	8	1C	1c	NN
cord-009318-zt1o1bcz	112	9	)	)	-RRB-
cord-009318-zt1o1bcz	112	10	,	,	,
cord-009318-zt1o1bcz	112	11	also	also	RB
cord-009318-zt1o1bcz	112	12	yielded	yield	VBD
cord-009318-zt1o1bcz	112	13	amplification	amplification	NN
cord-009318-zt1o1bcz	112	14	from	from	IN
cord-009318-zt1o1bcz	112	15	10	10	CD
cord-009318-zt1o1bcz	112	16	-	-	SYM
cord-009318-zt1o1bcz	112	17	11	11	CD
cord-009318-zt1o1bcz	112	18	min	min	NN
cord-009318-zt1o1bcz	112	19	;	;	:
cord-009318-zt1o1bcz	112	20	however	however	RB
cord-009318-zt1o1bcz	112	21	,	,	,
cord-009318-zt1o1bcz	112	22	we	-PRON-	PRP
cord-009318-zt1o1bcz	112	23	also	also	RB
cord-009318-zt1o1bcz	112	24	observed	observe	VBD
cord-009318-zt1o1bcz	112	25	a	a	DT
cord-009318-zt1o1bcz	112	26	non	non	JJ
cord-009318-zt1o1bcz	112	27	-	-	JJ
cord-009318-zt1o1bcz	112	28	specific	specific	JJ
cord-009318-zt1o1bcz	112	29	amplification	amplification	NN
cord-009318-zt1o1bcz	112	30	at	at	IN
cord-009318-zt1o1bcz	112	31	15	15	CD
cord-009318-zt1o1bcz	112	32	min	min	NN
cord-009318-zt1o1bcz	112	33	,	,	,
cord-009318-zt1o1bcz	112	34	defined	define	VBN
cord-009318-zt1o1bcz	112	35	as	as	IN
cord-009318-zt1o1bcz	112	36	having	have	VBG
cord-009318-zt1o1bcz	112	37	a	a	DT
cord-009318-zt1o1bcz	112	38	different	different	JJ
cord-009318-zt1o1bcz	112	39	T	t	NN
cord-009318-zt1o1bcz	112	40	m	m	NN
cord-009318-zt1o1bcz	112	41	than	than	IN
cord-009318-zt1o1bcz	112	42	the	the	DT
cord-009318-zt1o1bcz	112	43	specific	specific	JJ
cord-009318-zt1o1bcz	112	44	amplification	amplification	NN
cord-009318-zt1o1bcz	112	45	events	event	NNS
cord-009318-zt1o1bcz	112	46	(	(	-LRB-
cord-009318-zt1o1bcz	112	47	Figure	figure	NN
cord-009318-zt1o1bcz	112	48	1D	1d	CD
cord-009318-zt1o1bcz	112	49	)	)	-RRB-
cord-009318-zt1o1bcz	112	50	.	.	.
cord-009318-zt1o1bcz	113	1	This	this	DT
cord-009318-zt1o1bcz	113	2	indicated	indicate	VBD
cord-009318-zt1o1bcz	113	3	Bst	Bst	NNP
cord-009318-zt1o1bcz	113	4	3.0	3.0	NNP
cord-009318-zt1o1bcz	113	5	could	could	MD
cord-009318-zt1o1bcz	113	6	be	be	VB
cord-009318-zt1o1bcz	113	7	a	a	DT
cord-009318-zt1o1bcz	113	8	useful	useful	JJ
cord-009318-zt1o1bcz	113	9	model	model	NN
cord-009318-zt1o1bcz	113	10	for	for	IN
cord-009318-zt1o1bcz	113	11	studying	study	VBG
cord-009318-zt1o1bcz	113	12	non	non	JJ
cord-009318-zt1o1bcz	113	13	-	-	JJ
cord-009318-zt1o1bcz	113	14	specific	specific	JJ
cord-009318-zt1o1bcz	113	15	amplification	amplification	NN
cord-009318-zt1o1bcz	113	16	.	.	.
cord-009318-zt1o1bcz	114	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	114	2	observed	observe	VBD
cord-009318-zt1o1bcz	114	3	that	that	IN
cord-009318-zt1o1bcz	114	4	early	early	RB
cord-009318-zt1o1bcz	114	5	amplifying	amplify	VBG
cord-009318-zt1o1bcz	114	6	products	product	NNS
cord-009318-zt1o1bcz	114	7	corresponded	correspond	VBD
cord-009318-zt1o1bcz	114	8	to	to	IN
cord-009318-zt1o1bcz	114	9	specific	specific	JJ
cord-009318-zt1o1bcz	114	10	amplification	amplification	NN
cord-009318-zt1o1bcz	114	11	events	event	NNS
cord-009318-zt1o1bcz	114	12	,	,	,
cord-009318-zt1o1bcz	114	13	and	and	CC
cord-009318-zt1o1bcz	114	14	the	the	DT
cord-009318-zt1o1bcz	114	15	later	later	JJ
cord-009318-zt1o1bcz	114	16	products	product	NNS
cord-009318-zt1o1bcz	114	17	corresponded	correspond	VBD
cord-009318-zt1o1bcz	114	18	to	to	IN
cord-009318-zt1o1bcz	114	19	non	non	JJ
cord-009318-zt1o1bcz	114	20	-	-	JJ
cord-009318-zt1o1bcz	114	21	specific	specific	JJ
cord-009318-zt1o1bcz	114	22	amplification	amplification	NN
cord-009318-zt1o1bcz	114	23	,	,	,
cord-009318-zt1o1bcz	114	24	supporting	support	VBG
cord-009318-zt1o1bcz	114	25	our	-PRON-	PRP$
cord-009318-zt1o1bcz	114	26	prediction	prediction	NN
cord-009318-zt1o1bcz	114	27	that	that	IN
cord-009318-zt1o1bcz	114	28	we	-PRON-	PRP
cord-009318-zt1o1bcz	114	29	could	could	MD
cord-009318-zt1o1bcz	114	30	use	use	VB
cord-009318-zt1o1bcz	114	31	T	T	NNP
cord-009318-zt1o1bcz	114	32	m	m	NNP
cord-009318-zt1o1bcz	114	33	as	as	IN
cord-009318-zt1o1bcz	114	34	a	a	DT
cord-009318-zt1o1bcz	114	35	proxy	proxy	NN
cord-009318-zt1o1bcz	114	36	for	for	IN
cord-009318-zt1o1bcz	114	37	sequence	sequence	NN
cord-009318-zt1o1bcz	114	38	identity	identity	NN
cord-009318-zt1o1bcz	114	39	,	,	,
cord-009318-zt1o1bcz	114	40	as	as	IN
cord-009318-zt1o1bcz	114	41	is	be	VBZ
cord-009318-zt1o1bcz	114	42	common	common	JJ
cord-009318-zt1o1bcz	114	43	with	with	IN
cord-009318-zt1o1bcz	114	44	PCR	PCR	NNP
cord-009318-zt1o1bcz	114	45	and	and	CC
cord-009318-zt1o1bcz	114	46	has	have	VBZ
cord-009318-zt1o1bcz	114	47	been	be	VBN
cord-009318-zt1o1bcz	114	48	used	use	VBN
cord-009318-zt1o1bcz	114	49	previously	previously	RB
cord-009318-zt1o1bcz	114	50	in	in	IN
cord-009318-zt1o1bcz	114	51	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	114	52	(	(	-LRB-
cord-009318-zt1o1bcz	114	53	25	25	CD
cord-009318-zt1o1bcz	114	54	)	)	-RRB-
cord-009318-zt1o1bcz	114	55	(	(	-LRB-
cord-009318-zt1o1bcz	114	56	26	26	CD
cord-009318-zt1o1bcz	114	57	)	)	-RRB-
cord-009318-zt1o1bcz	114	58	(	(	-LRB-
cord-009318-zt1o1bcz	114	59	27	27	CD
cord-009318-zt1o1bcz	114	60	)	)	-RRB-
cord-009318-zt1o1bcz	114	61	(	(	-LRB-
cord-009318-zt1o1bcz	114	62	28	28	CD
cord-009318-zt1o1bcz	114	63	)	)	-RRB-
cord-009318-zt1o1bcz	114	64	(	(	-LRB-
cord-009318-zt1o1bcz	114	65	29	29	CD
cord-009318-zt1o1bcz	114	66	)	)	-RRB-
cord-009318-zt1o1bcz	114	67	.	.	.
cord-009318-zt1o1bcz	115	1	Using	use	VBG
cord-009318-zt1o1bcz	115	2	Bst	Bst	NNP
cord-009318-zt1o1bcz	115	3	3.0	3.0	NNP
cord-009318-zt1o1bcz	115	4	at	at	IN
cord-009318-zt1o1bcz	115	5	low	low	JJ
cord-009318-zt1o1bcz	115	6	concentrations	concentration	NNS
cord-009318-zt1o1bcz	115	7	of	of	IN
cord-009318-zt1o1bcz	115	8	target	target	NN
cord-009318-zt1o1bcz	115	9	is	be	VBZ
cord-009318-zt1o1bcz	115	10	a	a	DT
cord-009318-zt1o1bcz	115	11	useful	useful	JJ
cord-009318-zt1o1bcz	115	12	system	system	NN
cord-009318-zt1o1bcz	115	13	to	to	TO
cord-009318-zt1o1bcz	115	14	study	study	VB
cord-009318-zt1o1bcz	115	15	non	non	JJ
cord-009318-zt1o1bcz	115	16	-	-	JJ
cord-009318-zt1o1bcz	115	17	specific	specific	JJ
cord-009318-zt1o1bcz	115	18	amplification	amplification	NN
cord-009318-zt1o1bcz	115	19	.	.	.
cord-009318-zt1o1bcz	116	1	To	to	TO
cord-009318-zt1o1bcz	116	2	investigate	investigate	VB
cord-009318-zt1o1bcz	116	3	the	the	DT
cord-009318-zt1o1bcz	116	4	role	role	NN
cord-009318-zt1o1bcz	116	5	of	of	IN
cord-009318-zt1o1bcz	116	6	the	the	DT
cord-009318-zt1o1bcz	116	7	concentration	concentration	NN
cord-009318-zt1o1bcz	116	8	of	of	IN
cord-009318-zt1o1bcz	116	9	the	the	DT
cord-009318-zt1o1bcz	116	10	target	target	NN
cord-009318-zt1o1bcz	116	11	on	on	IN
cord-009318-zt1o1bcz	116	12	the	the	DT
cord-009318-zt1o1bcz	116	13	incidence	incidence	NN
cord-009318-zt1o1bcz	116	14	of	of	IN
cord-009318-zt1o1bcz	116	15	non	non	JJ
cord-009318-zt1o1bcz	116	16	-	-	JJ
cord-009318-zt1o1bcz	116	17	specific	specific	JJ
cord-009318-zt1o1bcz	116	18	amplification	amplification	NN
cord-009318-zt1o1bcz	116	19	,	,	,
cord-009318-zt1o1bcz	116	20	we	-PRON-	PRP
cord-009318-zt1o1bcz	116	21	performed	perform	VBD
cord-009318-zt1o1bcz	116	22	half	half	JJ
cord-009318-zt1o1bcz	116	23	-	-	HYPH
cord-009318-zt1o1bcz	116	24	log	log	NN
cord-009318-zt1o1bcz	116	25	dilutions	dilution	NNS
cord-009318-zt1o1bcz	116	26	of	of	IN
cord-009318-zt1o1bcz	116	27	template	template	NN
cord-009318-zt1o1bcz	116	28	from	from	IN
cord-009318-zt1o1bcz	116	29	10	10	CD
cord-009318-zt1o1bcz	116	30	to	to	IN
cord-009318-zt1o1bcz	116	31	0.316	0.316	CD
cord-009318-zt1o1bcz	116	32	cp	cp	NNP
cord-009318-zt1o1bcz	116	33	/	/	SYM
cord-009318-zt1o1bcz	116	34	l	l	NN
cord-009318-zt1o1bcz	116	35	.	.	.
cord-009318-zt1o1bcz	117	1	At	at	IN
cord-009318-zt1o1bcz	117	2	3.16	3.16	CD
cord-009318-zt1o1bcz	117	3	cp	cp	NNS
cord-009318-zt1o1bcz	117	4	/	/	SYM
cord-009318-zt1o1bcz	117	5	l	l	NN
cord-009318-zt1o1bcz	117	6	(	(	-LRB-
cord-009318-zt1o1bcz	117	7	Figure	figure	NN
cord-009318-zt1o1bcz	117	8	 	 	_SP
cord-009318-zt1o1bcz	117	9	1E	1e	NN
cord-009318-zt1o1bcz	117	10	and	and	CC
cord-009318-zt1o1bcz	117	11	F	F	NNP
cord-009318-zt1o1bcz	117	12	)	)	-RRB-
cord-009318-zt1o1bcz	117	13	,	,	,
cord-009318-zt1o1bcz	117	14	only	only	RB
cord-009318-zt1o1bcz	117	15	specific	specific	JJ
cord-009318-zt1o1bcz	117	16	amplification	amplification	NN
cord-009318-zt1o1bcz	117	17	occurred	occur	VBD
cord-009318-zt1o1bcz	117	18	(	(	-LRB-
cord-009318-zt1o1bcz	117	19	24	24	CD
cord-009318-zt1o1bcz	117	20	replicate	replicate	VBP
cord-009318-zt1o1bcz	117	21	wells	well	NNS
cord-009318-zt1o1bcz	117	22	/	/	SYM
cord-009318-zt1o1bcz	117	23	plate	plate	NN
cord-009318-zt1o1bcz	117	24	)	)	-RRB-
cord-009318-zt1o1bcz	117	25	.	.	.
cord-009318-zt1o1bcz	118	1	However	however	RB
cord-009318-zt1o1bcz	118	2	,	,	,
cord-009318-zt1o1bcz	118	3	once	once	RB
cord-009318-zt1o1bcz	118	4	template	template	NN
cord-009318-zt1o1bcz	118	5	concentrations	concentration	NNS
cord-009318-zt1o1bcz	118	6	reached	reach	VBD
cord-009318-zt1o1bcz	118	7	1	1	CD
cord-009318-zt1o1bcz	118	8	cp	cp	NN
cord-009318-zt1o1bcz	118	9	/	/	CC
cord-009318-zt1o1bcz	118	10	l	l	NN
cord-009318-zt1o1bcz	118	11	(	(	-LRB-
cord-009318-zt1o1bcz	118	12	Figure	figure	NN
cord-009318-zt1o1bcz	118	13	1	1	CD
cord-009318-zt1o1bcz	118	14	G	g	NN
cord-009318-zt1o1bcz	118	15	and	and	CC
cord-009318-zt1o1bcz	118	16	H	H	NNP
cord-009318-zt1o1bcz	118	17	)	)	-RRB-
cord-009318-zt1o1bcz	118	18	,	,	,
cord-009318-zt1o1bcz	118	19	non	non	JJ
cord-009318-zt1o1bcz	118	20	-	-	JJ
cord-009318-zt1o1bcz	118	21	specific	specific	JJ
cord-009318-zt1o1bcz	118	22	amplification	amplification	NN
cord-009318-zt1o1bcz	118	23	occurred	occur	VBD
cord-009318-zt1o1bcz	118	24	with	with	IN
cord-009318-zt1o1bcz	118	25	greater	great	JJR
cord-009318-zt1o1bcz	118	26	frequency	frequency	NN
cord-009318-zt1o1bcz	118	27	than	than	IN
cord-009318-zt1o1bcz	118	28	specific	specific	JJ
cord-009318-zt1o1bcz	118	29	amplification	amplification	NN
cord-009318-zt1o1bcz	118	30	(	(	-LRB-
cord-009318-zt1o1bcz	118	31	18	18	CD
cord-009318-zt1o1bcz	118	32	of	of	IN
cord-009318-zt1o1bcz	118	33	the	the	DT
cord-009318-zt1o1bcz	118	34	24	24	CD
cord-009318-zt1o1bcz	118	35	replicates	replicate	NNS
cord-009318-zt1o1bcz	118	36	generated	generate	VBD
cord-009318-zt1o1bcz	118	37	false	false	JJ
cord-009318-zt1o1bcz	118	38	positives	positive	NNS
cord-009318-zt1o1bcz	118	39	)	)	-RRB-
cord-009318-zt1o1bcz	118	40	.	.	.
cord-009318-zt1o1bcz	119	1	Similarly	similarly	RB
cord-009318-zt1o1bcz	119	2	,	,	,
cord-009318-zt1o1bcz	119	3	for	for	IN
cord-009318-zt1o1bcz	119	4	0.316	0.316	CD
cord-009318-zt1o1bcz	119	5	cp	cp	NNP
cord-009318-zt1o1bcz	119	6	/	/	SYM
cord-009318-zt1o1bcz	119	7	l	l	NNP
cord-009318-zt1o1bcz	119	8	(	(	-LRB-
cord-009318-zt1o1bcz	119	9	Figure	Figure	NNP
cord-009318-zt1o1bcz	119	10	1I	1I	NNP
cord-009318-zt1o1bcz	119	11	and	and	CC
cord-009318-zt1o1bcz	119	12	J	J	NNP
cord-009318-zt1o1bcz	119	13	)	)	-RRB-
cord-009318-zt1o1bcz	119	14	15	15	CD
cord-009318-zt1o1bcz	119	15	of	of	IN
cord-009318-zt1o1bcz	119	16	the	the	DT
cord-009318-zt1o1bcz	119	17	24	24	CD
cord-009318-zt1o1bcz	119	18	replicates	replicate	NNS
cord-009318-zt1o1bcz	119	19	generated	generate	VBD
cord-009318-zt1o1bcz	119	20	false	false	JJ
cord-009318-zt1o1bcz	119	21	positives	positive	NNS
cord-009318-zt1o1bcz	119	22	.	.	.
cord-009318-zt1o1bcz	120	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	120	2	next	next	RB
cord-009318-zt1o1bcz	120	3	ran	run	VBD
cord-009318-zt1o1bcz	120	4	the	the	DT
cord-009318-zt1o1bcz	120	5	same	same	JJ
cord-009318-zt1o1bcz	120	6	assay	assay	NN
cord-009318-zt1o1bcz	120	7	in	in	IN
cord-009318-zt1o1bcz	120	8	the	the	DT
cord-009318-zt1o1bcz	120	9	absence	absence	NN
cord-009318-zt1o1bcz	120	10	of	of	IN
cord-009318-zt1o1bcz	120	11	template	template	NN
cord-009318-zt1o1bcz	120	12	(	(	-LRB-
cord-009318-zt1o1bcz	120	13	no	no	DT
cord-009318-zt1o1bcz	120	14	-	-	HYPH
cord-009318-zt1o1bcz	120	15	template	template	NN
cord-009318-zt1o1bcz	120	16	control	control	NN
cord-009318-zt1o1bcz	120	17	,	,	,
cord-009318-zt1o1bcz	120	18	NTC	NTC	NNP
cord-009318-zt1o1bcz	120	19	)	)	-RRB-
cord-009318-zt1o1bcz	121	1	(	(	-LRB-
cord-009318-zt1o1bcz	121	2	Figure	figure	NN
cord-009318-zt1o1bcz	121	3	 	 	_SP
cord-009318-zt1o1bcz	121	4	1	1	CD
cord-009318-zt1o1bcz	121	5	K	K	NNP
cord-009318-zt1o1bcz	121	6	and	and	CC
cord-009318-zt1o1bcz	121	7	L	L	NNP
cord-009318-zt1o1bcz	121	8	)	)	-RRB-
cord-009318-zt1o1bcz	121	9	.	.	.
cord-009318-zt1o1bcz	122	1	Even	even	RB
cord-009318-zt1o1bcz	122	2	though	though	IN
cord-009318-zt1o1bcz	122	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	122	4	did	do	VBD
cord-009318-zt1o1bcz	122	5	not	not	RB
cord-009318-zt1o1bcz	122	6	expect	expect	VB
cord-009318-zt1o1bcz	122	7	amplification	amplification	NN
cord-009318-zt1o1bcz	122	8	,	,	,
cord-009318-zt1o1bcz	122	9	we	-PRON-	PRP
cord-009318-zt1o1bcz	122	10	observed	observe	VBD
cord-009318-zt1o1bcz	122	11	all	all	DT
cord-009318-zt1o1bcz	122	12	reactions	reaction	NNS
cord-009318-zt1o1bcz	122	13	amplified	amplify	VBN
cord-009318-zt1o1bcz	122	14	.	.	.
cord-009318-zt1o1bcz	123	1	A	a	DT
cord-009318-zt1o1bcz	123	2	total	total	NN
cord-009318-zt1o1bcz	123	3	of	of	IN
cord-009318-zt1o1bcz	123	4	44	44	CD
cord-009318-zt1o1bcz	123	5	of	of	IN
cord-009318-zt1o1bcz	123	6	45	45	CD
cord-009318-zt1o1bcz	123	7	replicates	replicate	NNS
cord-009318-zt1o1bcz	123	8	amplified	amplify	VBN
cord-009318-zt1o1bcz	123	9	at	at	IN
cord-009318-zt1o1bcz	123	10	a	a	DT
cord-009318-zt1o1bcz	123	11	T	t	NN
cord-009318-zt1o1bcz	123	12	m	m	NN
cord-009318-zt1o1bcz	123	13	of	of	IN
cord-009318-zt1o1bcz	123	14	91	91	CD
cord-009318-zt1o1bcz	124	1	•	•	NNP
cord-009318-zt1o1bcz	124	2	C	C	NNP
cord-009318-zt1o1bcz	124	3	,	,	,
cord-009318-zt1o1bcz	124	4	consistent	consistent	JJ
cord-009318-zt1o1bcz	124	5	with	with	IN
cord-009318-zt1o1bcz	124	6	the	the	DT
cord-009318-zt1o1bcz	124	7	T	T	NNP
cord-009318-zt1o1bcz	124	8	m	m	NN
cord-009318-zt1o1bcz	124	9	of	of	IN
cord-009318-zt1o1bcz	124	10	non	non	JJ
cord-009318-zt1o1bcz	124	11	-	-	JJ
cord-009318-zt1o1bcz	124	12	specific	specific	JJ
cord-009318-zt1o1bcz	124	13	amplification	amplification	NN
cord-009318-zt1o1bcz	124	14	in	in	IN
cord-009318-zt1o1bcz	124	15	the	the	DT
cord-009318-zt1o1bcz	124	16	presence	presence	NN
cord-009318-zt1o1bcz	124	17	of	of	IN
cord-009318-zt1o1bcz	124	18	template	template	NN
cord-009318-zt1o1bcz	124	19	.	.	.
cord-009318-zt1o1bcz	125	1	Although	although	IN
cord-009318-zt1o1bcz	125	2	it	-PRON-	PRP
cord-009318-zt1o1bcz	125	3	is	be	VBZ
cord-009318-zt1o1bcz	125	4	possible	possible	JJ
cord-009318-zt1o1bcz	125	5	for	for	IN
cord-009318-zt1o1bcz	125	6	a	a	DT
cord-009318-zt1o1bcz	125	7	reaction	reaction	NN
cord-009318-zt1o1bcz	125	8	to	to	TO
cord-009318-zt1o1bcz	125	9	generate	generate	VB
cord-009318-zt1o1bcz	125	10	multiple	multiple	JJ
cord-009318-zt1o1bcz	125	11	different	different	JJ
cord-009318-zt1o1bcz	125	12	non	non	JJ
cord-009318-zt1o1bcz	125	13	-	-	JJ
cord-009318-zt1o1bcz	125	14	specific	specific	JJ
cord-009318-zt1o1bcz	125	15	amplification	amplification	NN
cord-009318-zt1o1bcz	125	16	products	product	NNS
cord-009318-zt1o1bcz	125	17	,	,	,
cord-009318-zt1o1bcz	125	18	even	even	RB
cord-009318-zt1o1bcz	125	19	ones	one	NNS
cord-009318-zt1o1bcz	125	20	with	with	IN
cord-009318-zt1o1bcz	125	21	T	T	NNP
cord-009318-zt1o1bcz	125	22	m	m	NN
cord-009318-zt1o1bcz	125	23	matching	matching	NN
cord-009318-zt1o1bcz	125	24	to	to	IN
cord-009318-zt1o1bcz	125	25	the	the	DT
cord-009318-zt1o1bcz	125	26	specific	specific	JJ
cord-009318-zt1o1bcz	125	27	products	product	NNS
cord-009318-zt1o1bcz	125	28	,	,	,
cord-009318-zt1o1bcz	125	29	the	the	DT
cord-009318-zt1o1bcz	125	30	single	single	JJ
cord-009318-zt1o1bcz	125	31	amplicon	amplicon	NN
cord-009318-zt1o1bcz	125	32	observed	observe	VBD
cord-009318-zt1o1bcz	125	33	at	at	IN
cord-009318-zt1o1bcz	125	34	88	88	CD
cord-009318-zt1o1bcz	125	35	•	•	NNS
cord-009318-zt1o1bcz	126	1	C	C	NNP
cord-009318-zt1o1bcz	126	2	in	in	IN
cord-009318-zt1o1bcz	126	3	the	the	DT
cord-009318-zt1o1bcz	126	4	NTC	NTC	NNP
cord-009318-zt1o1bcz	126	5	was	be	VBD
cord-009318-zt1o1bcz	126	6	a	a	DT
cord-009318-zt1o1bcz	126	7	contaminant	contaminant	NN
cord-009318-zt1o1bcz	126	8	that	that	WDT
cord-009318-zt1o1bcz	126	9	appeared	appear	VBD
cord-009318-zt1o1bcz	126	10	to	to	TO
cord-009318-zt1o1bcz	126	11	have	have	VB
cord-009318-zt1o1bcz	126	12	the	the	DT
cord-009318-zt1o1bcz	126	13	same	same	JJ
cord-009318-zt1o1bcz	126	14	sequence	sequence	NN
cord-009318-zt1o1bcz	126	15	as	as	IN
cord-009318-zt1o1bcz	126	16	the	the	DT
cord-009318-zt1o1bcz	126	17	specific	specific	JJ
cord-009318-zt1o1bcz	126	18	products	product	NNS
cord-009318-zt1o1bcz	126	19	(	(	-LRB-
cord-009318-zt1o1bcz	126	20	Figure	figure	NN
cord-009318-zt1o1bcz	126	21	2A	2a	CD
cord-009318-zt1o1bcz	126	22	[	[	-LRB-
cord-009318-zt1o1bcz	126	23	well	well	UH
cord-009318-zt1o1bcz	126	24	F8	f8	NN
cord-009318-zt1o1bcz	126	25	]	]	-RRB-
cord-009318-zt1o1bcz	126	26	)	)	-RRB-
cord-009318-zt1o1bcz	126	27	.	.	.
cord-009318-zt1o1bcz	127	1	In	in	IN
cord-009318-zt1o1bcz	127	2	general	general	JJ
cord-009318-zt1o1bcz	127	3	,	,	,
cord-009318-zt1o1bcz	127	4	when	when	WRB
cord-009318-zt1o1bcz	127	5	the	the	DT
cord-009318-zt1o1bcz	127	6	specific	specific	JJ
cord-009318-zt1o1bcz	127	7	target	target	NN
cord-009318-zt1o1bcz	127	8	was	be	VBD
cord-009318-zt1o1bcz	127	9	present	present	JJ
cord-009318-zt1o1bcz	127	10	,	,	,
cord-009318-zt1o1bcz	127	11	it	-PRON-	PRP
cord-009318-zt1o1bcz	127	12	amplified	amplify	VBD
cord-009318-zt1o1bcz	127	13	sooner	soon	RBR
cord-009318-zt1o1bcz	127	14	and	and	CC
cord-009318-zt1o1bcz	127	15	outcompeted	outcompete	VBD
cord-009318-zt1o1bcz	127	16	the	the	DT
cord-009318-zt1o1bcz	127	17	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	127	18	amplification	amplification	NN
cord-009318-zt1o1bcz	127	19	,	,	,
cord-009318-zt1o1bcz	127	20	thereby	thereby	RB
cord-009318-zt1o1bcz	127	21	reducing	reduce	VBG
cord-009318-zt1o1bcz	127	22	the	the	DT
cord-009318-zt1o1bcz	127	23	number	number	NN
cord-009318-zt1o1bcz	127	24	of	of	IN
cord-009318-zt1o1bcz	127	25	observations	observation	NNS
cord-009318-zt1o1bcz	127	26	of	of	IN
cord-009318-zt1o1bcz	127	27	non	non	JJ
cord-009318-zt1o1bcz	127	28	-	-	JJ
cord-009318-zt1o1bcz	127	29	specific	specific	JJ
cord-009318-zt1o1bcz	127	30	amplification	amplification	NN
cord-009318-zt1o1bcz	127	31	.	.	.
cord-009318-zt1o1bcz	128	1	To	to	TO
cord-009318-zt1o1bcz	128	2	determine	determine	VB
cord-009318-zt1o1bcz	128	3	if	if	IN
cord-009318-zt1o1bcz	128	4	the	the	DT
cord-009318-zt1o1bcz	128	5	non	non	JJ
cord-009318-zt1o1bcz	128	6	-	-	JJ
cord-009318-zt1o1bcz	128	7	specific	specific	JJ
cord-009318-zt1o1bcz	128	8	amplification	amplification	NN
cord-009318-zt1o1bcz	128	9	was	be	VBD
cord-009318-zt1o1bcz	128	10	inherent	inherent	JJ
cord-009318-zt1o1bcz	128	11	to	to	IN
cord-009318-zt1o1bcz	128	12	the	the	DT
cord-009318-zt1o1bcz	128	13	polymerase	polymerase	NN
cord-009318-zt1o1bcz	128	14	or	or	CC
cord-009318-zt1o1bcz	128	15	a	a	DT
cord-009318-zt1o1bcz	128	16	consequence	consequence	NN
cord-009318-zt1o1bcz	128	17	of	of	IN
cord-009318-zt1o1bcz	128	18	buffer	buffer	NN
cord-009318-zt1o1bcz	128	19	selection	selection	NN
cord-009318-zt1o1bcz	128	20	,	,	,
cord-009318-zt1o1bcz	128	21	we	-PRON-	PRP
cord-009318-zt1o1bcz	128	22	conducted	conduct	VBD
cord-009318-zt1o1bcz	128	23	additional	additional	JJ
cord-009318-zt1o1bcz	128	24	studies	study	NNS
cord-009318-zt1o1bcz	128	25	using	use	VBG
cord-009318-zt1o1bcz	128	26	both	both	CC
cord-009318-zt1o1bcz	128	27	Bst	Bst	NNP
cord-009318-zt1o1bcz	128	28	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	128	29	(	(	-LRB-
cord-009318-zt1o1bcz	128	30	Supplementary	supplementary	JJ
cord-009318-zt1o1bcz	128	31	Figure	Figure	NNP
cord-009318-zt1o1bcz	128	32	S1	S1	NNP
cord-009318-zt1o1bcz	128	33	and	and	CC
cord-009318-zt1o1bcz	128	34	Table	Table	NNP
cord-009318-zt1o1bcz	128	35	S1	S1	NNP
cord-009318-zt1o1bcz	128	36	)	)	-RRB-
cord-009318-zt1o1bcz	128	37	.	.	.
cord-009318-zt1o1bcz	129	1	To	to	TO
cord-009318-zt1o1bcz	129	2	better	well	RBR
cord-009318-zt1o1bcz	129	3	understand	understand	VB
cord-009318-zt1o1bcz	129	4	non	non	JJ
cord-009318-zt1o1bcz	129	5	-	-	JJ
cord-009318-zt1o1bcz	129	6	specific	specific	JJ
cord-009318-zt1o1bcz	129	7	amplification	amplification	NN
cord-009318-zt1o1bcz	129	8	in	in	IN
cord-009318-zt1o1bcz	129	9	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	129	10	,	,	,
cord-009318-zt1o1bcz	129	11	we	-PRON-	PRP
cord-009318-zt1o1bcz	129	12	investigated	investigate	VBD
cord-009318-zt1o1bcz	129	13	the	the	DT
cord-009318-zt1o1bcz	129	14	sequence	sequence	NN
cord-009318-zt1o1bcz	129	15	identity	identity	NN
cord-009318-zt1o1bcz	129	16	of	of	IN
cord-009318-zt1o1bcz	129	17	the	the	DT
cord-009318-zt1o1bcz	129	18	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	129	19	products	product	NNS
cord-009318-zt1o1bcz	129	20	with	with	IN
cord-009318-zt1o1bcz	129	21	high	high	JJ
cord-009318-zt1o1bcz	129	22	T	t	NN
cord-009318-zt1o1bcz	129	23	m	m	NN
cord-009318-zt1o1bcz	129	24	using	use	VBG
cord-009318-zt1o1bcz	129	25	sequencing	sequencing	NN
cord-009318-zt1o1bcz	129	26	and	and	CC
cord-009318-zt1o1bcz	129	27	gel	gel	NN
cord-009318-zt1o1bcz	129	28	analysis	analysis	NN
cord-009318-zt1o1bcz	129	29	and	and	CC
cord-009318-zt1o1bcz	129	30	compared	compare	VBD
cord-009318-zt1o1bcz	129	31	them	-PRON-	PRP
cord-009318-zt1o1bcz	129	32	with	with	IN
cord-009318-zt1o1bcz	129	33	the	the	DT
cord-009318-zt1o1bcz	129	34	specific	specific	JJ
cord-009318-zt1o1bcz	129	35	products	product	NNS
cord-009318-zt1o1bcz	129	36	.	.	.
cord-009318-zt1o1bcz	130	1	The	the	DT
cord-009318-zt1o1bcz	130	2	T	t	NN
cord-009318-zt1o1bcz	130	3	m	m	NN
cord-009318-zt1o1bcz	130	4	of	of	IN
cord-009318-zt1o1bcz	130	5	specific	specific	JJ
cord-009318-zt1o1bcz	130	6	amplification	amplification	NN
cord-009318-zt1o1bcz	130	7	differed	differ	VBD
cord-009318-zt1o1bcz	130	8	between	between	IN
cord-009318-zt1o1bcz	130	9	the	the	DT
cord-009318-zt1o1bcz	130	10	two	two	CD
cord-009318-zt1o1bcz	130	11	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	130	12	tested	test	VBN
cord-009318-zt1o1bcz	130	13	.	.	.
cord-009318-zt1o1bcz	131	1	Specific	specific	JJ
cord-009318-zt1o1bcz	131	2	amplification	amplification	NN
cord-009318-zt1o1bcz	131	3	for	for	IN
cord-009318-zt1o1bcz	131	4	Bst	Bst	NNP
cord-009318-zt1o1bcz	131	5	2.0	2.0	CD
cord-009318-zt1o1bcz	131	6	had	have	VBD
cord-009318-zt1o1bcz	131	7	a	a	DT
cord-009318-zt1o1bcz	131	8	T	t	NN
cord-009318-zt1o1bcz	131	9	m	m	NN
cord-009318-zt1o1bcz	131	10	of	of	IN
cord-009318-zt1o1bcz	131	11	85.5	85.5	CD
cord-009318-zt1o1bcz	131	12	•	•	NNP
cord-009318-zt1o1bcz	131	13	C	C	NNP
cord-009318-zt1o1bcz	131	14	,	,	,
cord-009318-zt1o1bcz	131	15	whereas	whereas	IN
cord-009318-zt1o1bcz	131	16	specific	specific	JJ
cord-009318-zt1o1bcz	131	17	amplification	amplification	NN
cord-009318-zt1o1bcz	131	18	using	use	VBG
cord-009318-zt1o1bcz	131	19	Bst	Bst	NNP
cord-009318-zt1o1bcz	131	20	3.0	3.0	NNP
cord-009318-zt1o1bcz	131	21	had	have	VBD
cord-009318-zt1o1bcz	131	22	a	a	DT
cord-009318-zt1o1bcz	131	23	T	t	NN
cord-009318-zt1o1bcz	131	24	m	m	NN
cord-009318-zt1o1bcz	131	25	of	of	IN
cord-009318-zt1o1bcz	131	26	88	88	CD
cord-009318-zt1o1bcz	131	27	•	•	NN
cord-009318-zt1o1bcz	131	28	C	C	NNP
cord-009318-zt1o1bcz	131	29	,	,	,
cord-009318-zt1o1bcz	131	30	and	and	CC
cord-009318-zt1o1bcz	131	31	demonstrated	demonstrate	VBD
cord-009318-zt1o1bcz	131	32	non	non	JJ
cord-009318-zt1o1bcz	131	33	-	-	JJ
cord-009318-zt1o1bcz	131	34	specific	specific	JJ
cord-009318-zt1o1bcz	131	35	amplification	amplification	NN
cord-009318-zt1o1bcz	131	36	at	at	IN
cord-009318-zt1o1bcz	131	37	T	T	NNP
cord-009318-zt1o1bcz	131	38	m	m	NNP
cord-009318-zt1o1bcz	131	39	of	of	IN
cord-009318-zt1o1bcz	131	40	91	91	CD
cord-009318-zt1o1bcz	131	41	•	•	NNS
cord-009318-zt1o1bcz	131	42	C.	c.	NN
cord-009318-zt1o1bcz	132	1	The	the	DT
cord-009318-zt1o1bcz	132	2	non	non	JJ
cord-009318-zt1o1bcz	132	3	-	-	JJ
cord-009318-zt1o1bcz	132	4	specific	specific	JJ
cord-009318-zt1o1bcz	132	5	amplification	amplification	NN
cord-009318-zt1o1bcz	132	6	had	have	VBD
cord-009318-zt1o1bcz	132	7	identical	identical	JJ
cord-009318-zt1o1bcz	132	8	T	T	NNP
cord-009318-zt1o1bcz	132	9	m	m	NN
cord-009318-zt1o1bcz	132	10	to	to	IN
cord-009318-zt1o1bcz	132	11	amplification	amplification	NN
cord-009318-zt1o1bcz	132	12	in	in	IN
cord-009318-zt1o1bcz	132	13	absence	absence	NN
cord-009318-zt1o1bcz	132	14	of	of	IN
cord-009318-zt1o1bcz	132	15	template	template	NN
cord-009318-zt1o1bcz	132	16	(	(	-LRB-
cord-009318-zt1o1bcz	132	17	Figure	figure	NN
cord-009318-zt1o1bcz	132	18	1	1	CD
cord-009318-zt1o1bcz	132	19	K	K	NNP
cord-009318-zt1o1bcz	132	20	and	and	CC
cord-009318-zt1o1bcz	132	21	L	L	NNP
cord-009318-zt1o1bcz	132	22	)	)	-RRB-
cord-009318-zt1o1bcz	132	23	.	.	.
cord-009318-zt1o1bcz	133	1	Despite	despite	IN
cord-009318-zt1o1bcz	133	2	the	the	DT
cord-009318-zt1o1bcz	133	3	specific	specific	JJ
cord-009318-zt1o1bcz	133	4	amplification	amplification	NN
cord-009318-zt1o1bcz	133	5	products	product	NNS
cord-009318-zt1o1bcz	133	6	of	of	IN
cord-009318-zt1o1bcz	133	7	Bst	Bst	NNP
cord-009318-zt1o1bcz	133	8	2.0	2.0	NNP
cord-009318-zt1o1bcz	133	9	and	and	CC
cord-009318-zt1o1bcz	133	10	Bst	Bst	NNP
cord-009318-zt1o1bcz	133	11	3.0	3.0	NNP
cord-009318-zt1o1bcz	133	12	producing	produce	VBG
cord-009318-zt1o1bcz	133	13	similar	similar	JJ
cord-009318-zt1o1bcz	133	14	gel	gel	NN
cord-009318-zt1o1bcz	133	15	banding	banding	NN
cord-009318-zt1o1bcz	133	16	patterns	pattern	NNS
cord-009318-zt1o1bcz	133	17	(	(	-LRB-
cord-009318-zt1o1bcz	133	18	Figure	figure	NN
cord-009318-zt1o1bcz	133	19	3	3	CD
cord-009318-zt1o1bcz	133	20	)	)	-RRB-
cord-009318-zt1o1bcz	133	21	and	and	CC
cord-009318-zt1o1bcz	133	22	the	the	DT
cord-009318-zt1o1bcz	133	23	same	same	JJ
cord-009318-zt1o1bcz	133	24	sequencing	sequencing	NN
cord-009318-zt1o1bcz	133	25	results	result	NNS
cord-009318-zt1o1bcz	133	26	(	(	-LRB-
cord-009318-zt1o1bcz	133	27	see	see	VB
cord-009318-zt1o1bcz	133	28	Figure	figure	NN
cord-009318-zt1o1bcz	133	29	2B	2b	CD
cord-009318-zt1o1bcz	133	30	)	)	-RRB-
cord-009318-zt1o1bcz	133	31	,	,	,
cord-009318-zt1o1bcz	133	32	they	-PRON-	PRP
cord-009318-zt1o1bcz	133	33	had	have	VBD
cord-009318-zt1o1bcz	133	34	different	different	JJ
cord-009318-zt1o1bcz	133	35	T	t	NN
cord-009318-zt1o1bcz	133	36	m	m	NN
cord-009318-zt1o1bcz	133	37	(	(	-LRB-
cord-009318-zt1o1bcz	133	38	Figure	figure	NN
cord-009318-zt1o1bcz	133	39	1B	1b	NN
cord-009318-zt1o1bcz	133	40	and	and	CC
cord-009318-zt1o1bcz	133	41	D	d	NN
cord-009318-zt1o1bcz	133	42	,	,	,
cord-009318-zt1o1bcz	133	43	respectively	respectively	RB
cord-009318-zt1o1bcz	133	44	)	)	-RRB-
cord-009318-zt1o1bcz	133	45	.	.	.
cord-009318-zt1o1bcz	134	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	134	2	determined	determine	VBD
cord-009318-zt1o1bcz	134	3	the	the	DT
cord-009318-zt1o1bcz	134	4	difference	difference	NN
cord-009318-zt1o1bcz	134	5	in	in	IN
cord-009318-zt1o1bcz	134	6	T	T	NNP
cord-009318-zt1o1bcz	134	7	m	m	NNP
cord-009318-zt1o1bcz	134	8	was	be	VBD
cord-009318-zt1o1bcz	134	9	due	due	JJ
cord-009318-zt1o1bcz	134	10	to	to	IN
cord-009318-zt1o1bcz	134	11	differences	difference	NNS
cord-009318-zt1o1bcz	134	12	in	in	IN
cord-009318-zt1o1bcz	134	13	buffer	buffer	NN
cord-009318-zt1o1bcz	134	14	conditions	condition	NNS
cord-009318-zt1o1bcz	134	15	(	(	-LRB-
cord-009318-zt1o1bcz	134	16	Supplementary	supplementary	JJ
cord-009318-zt1o1bcz	134	17	Figure	Figure	NNP
cord-009318-zt1o1bcz	134	18	S1	S1	NNP
cord-009318-zt1o1bcz	134	19	and	and	CC
cord-009318-zt1o1bcz	134	20	Table	Table	NNP
cord-009318-zt1o1bcz	134	21	S1	S1	NNP
cord-009318-zt1o1bcz	134	22	)	)	-RRB-
cord-009318-zt1o1bcz	134	23	.	.	.
cord-009318-zt1o1bcz	135	1	In	in	IN
cord-009318-zt1o1bcz	135	2	all	all	DT
cord-009318-zt1o1bcz	135	3	bulk	bulk	JJ
cord-009318-zt1o1bcz	135	4	reactions	reaction	NNS
cord-009318-zt1o1bcz	135	5	,	,	,
cord-009318-zt1o1bcz	135	6	we	-PRON-	PRP
cord-009318-zt1o1bcz	135	7	observed	observe	VBD
cord-009318-zt1o1bcz	135	8	non	non	JJ
cord-009318-zt1o1bcz	135	9	-	-	JJ
cord-009318-zt1o1bcz	135	10	specific	specific	JJ
cord-009318-zt1o1bcz	135	11	products	product	NNS
cord-009318-zt1o1bcz	135	12	with	with	IN
cord-009318-zt1o1bcz	135	13	high	high	JJ
cord-009318-zt1o1bcz	135	14	Tm	Tm	NNP
cord-009318-zt1o1bcz	135	15	.	.	.
cord-009318-zt1o1bcz	136	1	This	this	DT
cord-009318-zt1o1bcz	136	2	was	be	VBD
cord-009318-zt1o1bcz	136	3	surprising	surprising	JJ
cord-009318-zt1o1bcz	136	4	because	because	IN
cord-009318-zt1o1bcz	136	5	in	in	IN
cord-009318-zt1o1bcz	136	6	PCR	PCR	NNP
cord-009318-zt1o1bcz	136	7	primer	primer	NN
cord-009318-zt1o1bcz	136	8	dimers	dimer	NNS
cord-009318-zt1o1bcz	136	9	have	have	VBP
cord-009318-zt1o1bcz	136	10	low	low	JJ
cord-009318-zt1o1bcz	136	11	Tm	Tm	NNP
cord-009318-zt1o1bcz	136	12	;	;	:
cord-009318-zt1o1bcz	136	13	moreover	moreover	RB
cord-009318-zt1o1bcz	136	14	,	,	,
cord-009318-zt1o1bcz	136	15	in	in	IN
cord-009318-zt1o1bcz	136	16	previous	previous	JJ
cord-009318-zt1o1bcz	136	17	demonstrations	demonstration	NNS
cord-009318-zt1o1bcz	136	18	of	of	IN
cord-009318-zt1o1bcz	136	19	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	136	20	,	,	,
cord-009318-zt1o1bcz	136	21	T	T	NNP
cord-009318-zt1o1bcz	136	22	m	m	NNP
cord-009318-zt1o1bcz	136	23	was	be	VBD
cord-009318-zt1o1bcz	136	24	lower	low	JJR
cord-009318-zt1o1bcz	136	25	for	for	IN
cord-009318-zt1o1bcz	136	26	non	non	JJ
cord-009318-zt1o1bcz	136	27	-	-	JJ
cord-009318-zt1o1bcz	136	28	specific	specific	JJ
cord-009318-zt1o1bcz	136	29	compared	compare	VBN
cord-009318-zt1o1bcz	136	30	with	with	IN
cord-009318-zt1o1bcz	136	31	specific	specific	JJ
cord-009318-zt1o1bcz	136	32	products	product	NNS
cord-009318-zt1o1bcz	136	33	(	(	-LRB-
cord-009318-zt1o1bcz	136	34	27	27	CD
cord-009318-zt1o1bcz	136	35	)	)	-RRB-
cord-009318-zt1o1bcz	136	36	.	.	.
cord-009318-zt1o1bcz	137	1	Thus	thus	RB
cord-009318-zt1o1bcz	137	2	,	,	,
cord-009318-zt1o1bcz	137	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	137	4	investigated	investigate	VBD
cord-009318-zt1o1bcz	137	5	the	the	DT
cord-009318-zt1o1bcz	137	6	sequence	sequence	NN
cord-009318-zt1o1bcz	137	7	identity	identity	NN
cord-009318-zt1o1bcz	137	8	of	of	IN
cord-009318-zt1o1bcz	137	9	the	the	DT
cord-009318-zt1o1bcz	137	10	non	non	JJ
cord-009318-zt1o1bcz	137	11	-	-	JJ
cord-009318-zt1o1bcz	137	12	specific	specific	JJ
cord-009318-zt1o1bcz	137	13	product	product	NN
cord-009318-zt1o1bcz	137	14	with	with	IN
cord-009318-zt1o1bcz	137	15	high	high	JJ
cord-009318-zt1o1bcz	137	16	T	t	NN
cord-009318-zt1o1bcz	137	17	m	m	NN
cord-009318-zt1o1bcz	137	18	.	.	.
cord-009318-zt1o1bcz	138	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	138	2	ran	run	VBD
cord-009318-zt1o1bcz	138	3	the	the	DT
cord-009318-zt1o1bcz	138	4	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	138	5	products	product	NNS
cord-009318-zt1o1bcz	138	6	on	on	IN
cord-009318-zt1o1bcz	138	7	a	a	DT
cord-009318-zt1o1bcz	138	8	gel	gel	NN
cord-009318-zt1o1bcz	138	9	and	and	CC
cord-009318-zt1o1bcz	138	10	observed	observe	VBD
cord-009318-zt1o1bcz	138	11	that	that	IN
cord-009318-zt1o1bcz	138	12	the	the	DT
cord-009318-zt1o1bcz	138	13	characteristic	characteristic	JJ
cord-009318-zt1o1bcz	138	14	pattern	pattern	NN
cord-009318-zt1o1bcz	138	15	of	of	IN
cord-009318-zt1o1bcz	138	16	the	the	DT
cord-009318-zt1o1bcz	138	17	specific	specific	JJ
cord-009318-zt1o1bcz	138	18	amplification	amplification	NN
cord-009318-zt1o1bcz	138	19	products	product	NNS
cord-009318-zt1o1bcz	138	20	differed	differ	VBD
cord-009318-zt1o1bcz	138	21	substantially	substantially	RB
cord-009318-zt1o1bcz	138	22	from	from	IN
cord-009318-zt1o1bcz	138	23	the	the	DT
cord-009318-zt1o1bcz	138	24	banding	banding	NN
cord-009318-zt1o1bcz	138	25	pattern	pattern	NN
cord-009318-zt1o1bcz	138	26	seen	see	VBN
cord-009318-zt1o1bcz	138	27	in	in	IN
cord-009318-zt1o1bcz	138	28	the	the	DT
cord-009318-zt1o1bcz	138	29	high	high	JJ
cord-009318-zt1o1bcz	138	30	-	-	HYPH
cord-009318-zt1o1bcz	138	31	T	t	NN
cord-009318-zt1o1bcz	138	32	m	m	NN
cord-009318-zt1o1bcz	138	33	non	non	JJ
cord-009318-zt1o1bcz	138	34	-	-	JJ
cord-009318-zt1o1bcz	138	35	specific	specific	JJ
cord-009318-zt1o1bcz	138	36	products	product	NNS
cord-009318-zt1o1bcz	138	37	(	(	-LRB-
cord-009318-zt1o1bcz	138	38	Figure	figure	NN
cord-009318-zt1o1bcz	138	39	3	3	CD
cord-009318-zt1o1bcz	138	40	)	)	-RRB-
cord-009318-zt1o1bcz	138	41	.	.	.
cord-009318-zt1o1bcz	139	1	Interestingly	interestingly	RB
cord-009318-zt1o1bcz	139	2	,	,	,
cord-009318-zt1o1bcz	139	3	the	the	DT
cord-009318-zt1o1bcz	139	4	high	high	JJ
cord-009318-zt1o1bcz	139	5	-	-	HYPH
cord-009318-zt1o1bcz	139	6	T	t	NN
cord-009318-zt1o1bcz	139	7	m	m	NN
cord-009318-zt1o1bcz	139	8	non	non	JJ
cord-009318-zt1o1bcz	139	9	-	-	JJ
cord-009318-zt1o1bcz	139	10	specific	specific	JJ
cord-009318-zt1o1bcz	139	11	product	product	NN
cord-009318-zt1o1bcz	139	12	had	have	VBD
cord-009318-zt1o1bcz	139	13	a	a	DT
cord-009318-zt1o1bcz	139	14	ladder	ladder	NN
cord-009318-zt1o1bcz	139	15	pattern	pattern	NN
cord-009318-zt1o1bcz	139	16	resembling	resemble	VBG
cord-009318-zt1o1bcz	139	17	that	that	DT
cord-009318-zt1o1bcz	139	18	of	of	IN
cord-009318-zt1o1bcz	139	19	specific	specific	JJ
cord-009318-zt1o1bcz	139	20	LAMP	lamp	NN
cord-009318-zt1o1bcz	139	21	products	product	NNS
cord-009318-zt1o1bcz	139	22	.	.	.
cord-009318-zt1o1bcz	140	1	To	to	TO
cord-009318-zt1o1bcz	140	2	determine	determine	VB
cord-009318-zt1o1bcz	140	3	the	the	DT
cord-009318-zt1o1bcz	140	4	identity	identity	NN
cord-009318-zt1o1bcz	140	5	of	of	IN
cord-009318-zt1o1bcz	140	6	the	the	DT
cord-009318-zt1o1bcz	140	7	high	high	JJ
cord-009318-zt1o1bcz	140	8	-	-	HYPH
cord-009318-zt1o1bcz	140	9	T	t	NN
cord-009318-zt1o1bcz	140	10	m	m	NN
cord-009318-zt1o1bcz	140	11	non	non	JJ
cord-009318-zt1o1bcz	140	12	-	-	JJ
cord-009318-zt1o1bcz	140	13	specific	specific	JJ
cord-009318-zt1o1bcz	140	14	products	product	NNS
cord-009318-zt1o1bcz	140	15	,	,	,
cord-009318-zt1o1bcz	140	16	we	-PRON-	PRP
cord-009318-zt1o1bcz	140	17	performed	perform	VBD
cord-009318-zt1o1bcz	140	18	next	next	JJ
cord-009318-zt1o1bcz	140	19	generation	generation	NN
cord-009318-zt1o1bcz	140	20	sequencing	sequencing	NN
cord-009318-zt1o1bcz	140	21	(	(	-LRB-
cord-009318-zt1o1bcz	140	22	NGS	NGS	NNP
cord-009318-zt1o1bcz	140	23	)	)	-RRB-
cord-009318-zt1o1bcz	140	24	.	.	.
cord-009318-zt1o1bcz	141	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	141	2	observed	observe	VBD
cord-009318-zt1o1bcz	141	3	that	that	IN
cord-009318-zt1o1bcz	141	4	the	the	DT
cord-009318-zt1o1bcz	141	5	non	non	JJ
cord-009318-zt1o1bcz	141	6	-	-	JJ
cord-009318-zt1o1bcz	141	7	specific	specific	JJ
cord-009318-zt1o1bcz	141	8	products	product	NNS
cord-009318-zt1o1bcz	141	9	lacked	lack	VBD
cord-009318-zt1o1bcz	141	10	the	the	DT
cord-009318-zt1o1bcz	141	11	corresponding	corresponding	JJ
cord-009318-zt1o1bcz	141	12	target	target	NN
cord-009318-zt1o1bcz	141	13	sequence	sequence	NN
cord-009318-zt1o1bcz	141	14	and	and	CC
cord-009318-zt1o1bcz	141	15	identified	identify	VBD
cord-009318-zt1o1bcz	141	16	the	the	DT
cord-009318-zt1o1bcz	141	17	product	product	NN
cord-009318-zt1o1bcz	141	18	as	as	IN
cord-009318-zt1o1bcz	141	19	a	a	DT
cord-009318-zt1o1bcz	141	20	mixture	mixture	NN
cord-009318-zt1o1bcz	141	21	of	of	IN
cord-009318-zt1o1bcz	141	22	full	full	JJ
cord-009318-zt1o1bcz	141	23	-	-	HYPH
cord-009318-zt1o1bcz	141	24	length	length	NN
cord-009318-zt1o1bcz	141	25	FIP	fip	NN
cord-009318-zt1o1bcz	141	26	,	,	,
cord-009318-zt1o1bcz	141	27	BIP	BIP	NNP
cord-009318-zt1o1bcz	141	28	and	and	CC
cord-009318-zt1o1bcz	141	29	their	-PRON-	PRP$
cord-009318-zt1o1bcz	141	30	complements	complement	NNS
cord-009318-zt1o1bcz	141	31	,	,	,
cord-009318-zt1o1bcz	141	32	as	as	RB
cord-009318-zt1o1bcz	141	33	well	well	RB
cord-009318-zt1o1bcz	141	34	as	as	IN
cord-009318-zt1o1bcz	141	35	fragments	fragment	NNS
cord-009318-zt1o1bcz	141	36	of	of	IN
cord-009318-zt1o1bcz	141	37	BIP	BIP	NNP
cord-009318-zt1o1bcz	141	38	(	(	-LRB-
cord-009318-zt1o1bcz	141	39	Figure	figure	NN
cord-009318-zt1o1bcz	141	40	2A	2a	CD
cord-009318-zt1o1bcz	141	41	)	)	-RRB-
cord-009318-zt1o1bcz	141	42	.	.	.
cord-009318-zt1o1bcz	142	1	To	to	TO
cord-009318-zt1o1bcz	142	2	confirm	confirm	VB
cord-009318-zt1o1bcz	142	3	the	the	DT
cord-009318-zt1o1bcz	142	4	sequence	sequence	NN
cord-009318-zt1o1bcz	142	5	identity	identity	NN
cord-009318-zt1o1bcz	142	6	of	of	IN
cord-009318-zt1o1bcz	142	7	the	the	DT
cord-009318-zt1o1bcz	142	8	amplicon	amplicon	NN
cord-009318-zt1o1bcz	142	9	,	,	,
cord-009318-zt1o1bcz	142	10	we	-PRON-	PRP
cord-009318-zt1o1bcz	142	11	targeted	target	VBD
cord-009318-zt1o1bcz	142	12	the	the	DT
cord-009318-zt1o1bcz	142	13	FIP	FIP	NNP
cord-009318-zt1o1bcz	142	14	and	and	CC
cord-009318-zt1o1bcz	142	15	BIP	BIP	NNP
cord-009318-zt1o1bcz	142	16	regions	region	NNS
cord-009318-zt1o1bcz	142	17	using	use	VBG
cord-009318-zt1o1bcz	142	18	several	several	JJ
cord-009318-zt1o1bcz	142	19	restriction	restriction	NN
cord-009318-zt1o1bcz	142	20	endonucleases	endonuclease	NNS
cord-009318-zt1o1bcz	142	21	.	.	.
cord-009318-zt1o1bcz	143	1	Digestion	digestion	NN
cord-009318-zt1o1bcz	143	2	of	of	IN
cord-009318-zt1o1bcz	143	3	the	the	DT
cord-009318-zt1o1bcz	143	4	specific	specific	JJ
cord-009318-zt1o1bcz	143	5	and	and	CC
cord-009318-zt1o1bcz	143	6	non	non	JJ
cord-009318-zt1o1bcz	143	7	-	-	JJ
cord-009318-zt1o1bcz	143	8	specific	specific	JJ
cord-009318-zt1o1bcz	143	9	products	product	NNS
cord-009318-zt1o1bcz	143	10	resulted	result	VBD
cord-009318-zt1o1bcz	143	11	in	in	IN
cord-009318-zt1o1bcz	143	12	different	different	JJ
cord-009318-zt1o1bcz	143	13	banding	banding	NN
cord-009318-zt1o1bcz	143	14	patterns	pattern	NNS
cord-009318-zt1o1bcz	143	15	than	than	IN
cord-009318-zt1o1bcz	143	16	the	the	DT
cord-009318-zt1o1bcz	143	17	undigested	undigested	JJ
cord-009318-zt1o1bcz	143	18	samples	sample	NNS
cord-009318-zt1o1bcz	143	19	,	,	,
cord-009318-zt1o1bcz	143	20	and	and	CC
cord-009318-zt1o1bcz	143	21	was	be	VBD
cord-009318-zt1o1bcz	143	22	consistent	consistent	JJ
cord-009318-zt1o1bcz	143	23	with	with	IN
cord-009318-zt1o1bcz	143	24	the	the	DT
cord-009318-zt1o1bcz	143	25	presence	presence	NN
cord-009318-zt1o1bcz	143	26	of	of	IN
cord-009318-zt1o1bcz	143	27	both	both	CC
cord-009318-zt1o1bcz	143	28	FIP	FIP	NNP
cord-009318-zt1o1bcz	143	29	and	and	CC
cord-009318-zt1o1bcz	143	30	BIP	BIP	NNP
cord-009318-zt1o1bcz	143	31	endonuclease	endonuclease	NN
cord-009318-zt1o1bcz	143	32	recognition	recognition	NN
cord-009318-zt1o1bcz	143	33	sites	site	NNS
cord-009318-zt1o1bcz	143	34	within	within	IN
cord-009318-zt1o1bcz	143	35	the	the	DT
cord-009318-zt1o1bcz	143	36	sequence	sequence	NN
cord-009318-zt1o1bcz	143	37	(	(	-LRB-
cord-009318-zt1o1bcz	143	38	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	143	39	Figure	Figure	NNP
cord-009318-zt1o1bcz	143	40	S2	s2	NN
cord-009318-zt1o1bcz	143	41	)	)	-RRB-
cord-009318-zt1o1bcz	143	42	.	.	.
cord-009318-zt1o1bcz	144	1	Specific	specific	JJ
cord-009318-zt1o1bcz	144	2	amplification	amplification	NN
cord-009318-zt1o1bcz	144	3	products	product	NNS
cord-009318-zt1o1bcz	144	4	were	be	VBD
cord-009318-zt1o1bcz	144	5	47	47	CD
cord-009318-zt1o1bcz	144	6	%	%	NN
cord-009318-zt1o1bcz	144	7	GC	gc	NN
cord-009318-zt1o1bcz	144	8	;	;	:
cord-009318-zt1o1bcz	144	9	non	non	JJ
cord-009318-zt1o1bcz	144	10	-	-	JJ
cord-009318-zt1o1bcz	144	11	specific	specific	JJ
cord-009318-zt1o1bcz	144	12	amplification	amplification	NN
cord-009318-zt1o1bcz	144	13	products	product	NNS
cord-009318-zt1o1bcz	144	14	were	be	VBD
cord-009318-zt1o1bcz	144	15	53	53	CD
cord-009318-zt1o1bcz	144	16	%	%	NN
cord-009318-zt1o1bcz	144	17	GC	GC	NNP
cord-009318-zt1o1bcz	144	18	.	.	.
cord-009318-zt1o1bcz	145	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	145	2	hypothesize	hypothesize	VBP
cord-009318-zt1o1bcz	145	3	a	a	DT
cord-009318-zt1o1bcz	145	4	mechanism	mechanism	NN
cord-009318-zt1o1bcz	145	5	for	for	IN
cord-009318-zt1o1bcz	145	6	the	the	DT
cord-009318-zt1o1bcz	145	7	formation	formation	NN
cord-009318-zt1o1bcz	145	8	of	of	IN
cord-009318-zt1o1bcz	145	9	the	the	DT
cord-009318-zt1o1bcz	145	10	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	145	11	product	product	NN
cord-009318-zt1o1bcz	145	12	with	with	IN
cord-009318-zt1o1bcz	145	13	high	high	JJ
cord-009318-zt1o1bcz	145	14	T	t	NN
cord-009318-zt1o1bcz	145	15	m	m	NN
cord-009318-zt1o1bcz	145	16	originating	originating	NN
cord-009318-zt1o1bcz	145	17	as	as	IN
cord-009318-zt1o1bcz	145	18	a	a	DT
cord-009318-zt1o1bcz	145	19	consequence	consequence	NN
cord-009318-zt1o1bcz	145	20	of	of	IN
cord-009318-zt1o1bcz	145	21	interactions	interaction	NNS
cord-009318-zt1o1bcz	145	22	of	of	IN
cord-009318-zt1o1bcz	145	23	the	the	DT
cord-009318-zt1o1bcz	145	24	Bst	Bst	NNP
cord-009318-zt1o1bcz	145	25	polymerase	polymerase	NN
cord-009318-zt1o1bcz	145	26	and	and	CC
cord-009318-zt1o1bcz	145	27	LAMP	lamp	NN
cord-009318-zt1o1bcz	145	28	inner	inner	JJ
cord-009318-zt1o1bcz	145	29	primers	primer	NNS
cord-009318-zt1o1bcz	145	30	.	.	.
cord-009318-zt1o1bcz	146	1	Other	other	JJ
cord-009318-zt1o1bcz	146	2	potential	potential	JJ
cord-009318-zt1o1bcz	146	3	mechanisms	mechanism	NNS
cord-009318-zt1o1bcz	146	4	include	include	VBP
cord-009318-zt1o1bcz	146	5	LIMA	LIMA	NNP
cord-009318-zt1o1bcz	146	6	(	(	-LRB-
cord-009318-zt1o1bcz	146	7	30	30	CD
cord-009318-zt1o1bcz	146	8	)	)	-RRB-
cord-009318-zt1o1bcz	146	9	and	and	CC
cord-009318-zt1o1bcz	146	10	UIMA	UIMA	NNP
cord-009318-zt1o1bcz	146	11	(	(	-LRB-
cord-009318-zt1o1bcz	146	12	31	31	CD
cord-009318-zt1o1bcz	146	13	)	)	-RRB-
cord-009318-zt1o1bcz	146	14	,	,	,
cord-009318-zt1o1bcz	146	15	but	but	CC
cord-009318-zt1o1bcz	146	16	are	be	VBP
cord-009318-zt1o1bcz	146	17	inconsistent	inconsistent	JJ
cord-009318-zt1o1bcz	146	18	with	with	IN
cord-009318-zt1o1bcz	146	19	our	-PRON-	PRP$
cord-009318-zt1o1bcz	146	20	sequencing	sequencing	NN
cord-009318-zt1o1bcz	146	21	results	result	NNS
cord-009318-zt1o1bcz	146	22	,	,	,
cord-009318-zt1o1bcz	146	23	which	which	WDT
cord-009318-zt1o1bcz	146	24	observe	observe	VBP
cord-009318-zt1o1bcz	146	25	nearly	nearly	RB
cord-009318-zt1o1bcz	146	26	equal	equal	JJ
cord-009318-zt1o1bcz	146	27	reads	read	NNS
cord-009318-zt1o1bcz	146	28	of	of	IN
cord-009318-zt1o1bcz	146	29	the	the	DT
cord-009318-zt1o1bcz	146	30	forward	forward	NN
cord-009318-zt1o1bcz	146	31	and	and	CC
cord-009318-zt1o1bcz	146	32	reverse	reverse	VB
cord-009318-zt1o1bcz	146	33	strand	strand	NN
cord-009318-zt1o1bcz	146	34	as	as	IN
cord-009318-zt1o1bcz	146	35	measured	measure	VBN
cord-009318-zt1o1bcz	146	36	by	by	IN
cord-009318-zt1o1bcz	146	37	counting	count	VBG
cord-009318-zt1o1bcz	146	38	the	the	DT
cord-009318-zt1o1bcz	146	39	complementary	complementary	JJ
cord-009318-zt1o1bcz	146	40	sequences	sequence	NNS
cord-009318-zt1o1bcz	146	41	between	between	IN
cord-009318-zt1o1bcz	146	42	each	each	DT
cord-009318-zt1o1bcz	146	43	junction	junction	NN
cord-009318-zt1o1bcz	146	44	.	.	.
cord-009318-zt1o1bcz	147	1	Our	-PRON-	PRP$
cord-009318-zt1o1bcz	147	2	proposed	propose	VBN
cord-009318-zt1o1bcz	147	3	mechanism	mechanism	NN
cord-009318-zt1o1bcz	147	4	requires	require	VBZ
cord-009318-zt1o1bcz	147	5	properties	property	NNS
cord-009318-zt1o1bcz	147	6	that	that	WDT
cord-009318-zt1o1bcz	147	7	have	have	VBP
cord-009318-zt1o1bcz	147	8	been	be	VBN
cord-009318-zt1o1bcz	147	9	observed	observe	VBN
cord-009318-zt1o1bcz	147	10	with	with	IN
cord-009318-zt1o1bcz	147	11	Bst	Bst	NNP
cord-009318-zt1o1bcz	147	12	enzymes	enzyme	NNS
cord-009318-zt1o1bcz	147	13	:	:	:
cord-009318-zt1o1bcz	147	14	a	a	DT
cord-009318-zt1o1bcz	147	15	strand	strand	NN
cord-009318-zt1o1bcz	147	16	-	-	HYPH
cord-009318-zt1o1bcz	147	17	displacing	displace	VBG
cord-009318-zt1o1bcz	147	18	polymerase	polymerase	NN
cord-009318-zt1o1bcz	147	19	lacking	lack	VBG
cord-009318-zt1o1bcz	147	20	3	3	CD
cord-009318-zt1o1bcz	147	21	-5	-5	NN
cord-009318-zt1o1bcz	147	22	exonuclease	exonuclease	NN
cord-009318-zt1o1bcz	147	23	activity	activity	NN
cord-009318-zt1o1bcz	147	24	--	--	:
cord-009318-zt1o1bcz	147	25	common	common	JJ
cord-009318-zt1o1bcz	147	26	to	to	IN
cord-009318-zt1o1bcz	147	27	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	147	28	from	from	IN
cord-009318-zt1o1bcz	147	29	thermophilic	thermophilic	JJ
cord-009318-zt1o1bcz	147	30	bacteria	bacteria	NNS
cord-009318-zt1o1bcz	147	31	(	(	-LRB-
cord-009318-zt1o1bcz	147	32	32	32	CD
cord-009318-zt1o1bcz	147	33	,	,	,
cord-009318-zt1o1bcz	147	34	33	33	CD
cord-009318-zt1o1bcz	147	35	)	)	-RRB-
cord-009318-zt1o1bcz	147	36	,	,	,
cord-009318-zt1o1bcz	147	37	template	template	NN
cord-009318-zt1o1bcz	147	38	switching	switch	VBG
cord-009318-zt1o1bcz	147	39	ability	ability	NN
cord-009318-zt1o1bcz	147	40	to	to	TO
cord-009318-zt1o1bcz	147	41	allow	allow	VB
cord-009318-zt1o1bcz	147	42	synthesis	synthesis	NN
cord-009318-zt1o1bcz	147	43	across	across	IN
cord-009318-zt1o1bcz	147	44	a	a	DT
cord-009318-zt1o1bcz	147	45	discontinuous	discontinuous	JJ
cord-009318-zt1o1bcz	147	46	template	template	NN
cord-009318-zt1o1bcz	147	47	(	(	-LRB-
cord-009318-zt1o1bcz	147	48	33	33	CD
cord-009318-zt1o1bcz	147	49	)	)	-RRB-
cord-009318-zt1o1bcz	147	50	,	,	,
cord-009318-zt1o1bcz	147	51	terminal	terminal	JJ
cord-009318-zt1o1bcz	147	52	transferase	transferase	NN
cord-009318-zt1o1bcz	147	53	activity	activity	NN
cord-009318-zt1o1bcz	147	54	,	,	,
cord-009318-zt1o1bcz	147	55	or	or	CC
cord-009318-zt1o1bcz	147	56	the	the	DT
cord-009318-zt1o1bcz	147	57	ability	ability	NN
cord-009318-zt1o1bcz	147	58	to	to	TO
cord-009318-zt1o1bcz	147	59	perform	perform	VB
cord-009318-zt1o1bcz	147	60	nontemplated	nontemplate	VBN
cord-009318-zt1o1bcz	147	61	synthesis	synthesis	NN
cord-009318-zt1o1bcz	147	62	(	(	-LRB-
cord-009318-zt1o1bcz	147	63	32	32	CD
cord-009318-zt1o1bcz	147	64	,	,	,
cord-009318-zt1o1bcz	147	65	(	(	-LRB-
cord-009318-zt1o1bcz	147	66	34	34	CD
cord-009318-zt1o1bcz	147	67	)	)	-RRB-
cord-009318-zt1o1bcz	147	68	(	(	-LRB-
cord-009318-zt1o1bcz	147	69	35	35	CD
cord-009318-zt1o1bcz	147	70	)	)	-RRB-
cord-009318-zt1o1bcz	147	71	.	.	.
cord-009318-zt1o1bcz	148	1	Briefly	briefly	RB
cord-009318-zt1o1bcz	148	2	,	,	,
cord-009318-zt1o1bcz	148	3	the	the	DT
cord-009318-zt1o1bcz	148	4	non	non	JJ
cord-009318-zt1o1bcz	148	5	-	-	JJ
cord-009318-zt1o1bcz	148	6	specific	specific	JJ
cord-009318-zt1o1bcz	148	7	product	product	NN
cord-009318-zt1o1bcz	148	8	likely	likely	RB
cord-009318-zt1o1bcz	148	9	arises	arise	VBZ
cord-009318-zt1o1bcz	148	10	from	from	IN
cord-009318-zt1o1bcz	148	11	extension	extension	NN
cord-009318-zt1o1bcz	148	12	of	of	IN
cord-009318-zt1o1bcz	148	13	a	a	DT
cord-009318-zt1o1bcz	148	14	low	low	JJ
cord-009318-zt1o1bcz	148	15	probability	probability	NN
cord-009318-zt1o1bcz	148	16	homo	homo	NN
cord-009318-zt1o1bcz	148	17	-	-	HYPH
cord-009318-zt1o1bcz	148	18	dimerization	dimerization	NN
cord-009318-zt1o1bcz	148	19	of	of	IN
cord-009318-zt1o1bcz	148	20	the	the	DT
cord-009318-zt1o1bcz	148	21	Backward	Backward	NNP
cord-009318-zt1o1bcz	148	22	Inner	Inner	NNP
cord-009318-zt1o1bcz	148	23	Primer	Primer	NNP
cord-009318-zt1o1bcz	148	24	(	(	-LRB-
cord-009318-zt1o1bcz	148	25	BIP	BIP	NNP
cord-009318-zt1o1bcz	148	26	)	)	-RRB-
cord-009318-zt1o1bcz	148	27	,	,	,
cord-009318-zt1o1bcz	148	28	followed	follow	VBN
cord-009318-zt1o1bcz	148	29	by	by	IN
cord-009318-zt1o1bcz	148	30	elongation	elongation	NN
cord-009318-zt1o1bcz	148	31	across	across	IN
cord-009318-zt1o1bcz	148	32	a	a	DT
cord-009318-zt1o1bcz	148	33	discontinuous	discontinuous	JJ
cord-009318-zt1o1bcz	148	34	junction	junction	NN
cord-009318-zt1o1bcz	148	35	(	(	-LRB-
cord-009318-zt1o1bcz	148	36	'	'	``
cord-009318-zt1o1bcz	148	37	template	template	NN
cord-009318-zt1o1bcz	148	38	switching	switching	NN
cord-009318-zt1o1bcz	148	39	'	'	''
cord-009318-zt1o1bcz	148	40	)	)	-RRB-
cord-009318-zt1o1bcz	148	41	to	to	TO
cord-009318-zt1o1bcz	148	42	form	form	VB
cord-009318-zt1o1bcz	148	43	a	a	DT
cord-009318-zt1o1bcz	148	44	double	double	RB
cord-009318-zt1o1bcz	148	45	-	-	HYPH
cord-009318-zt1o1bcz	148	46	stranded	stranded	JJ
cord-009318-zt1o1bcz	148	47	product	product	NN
cord-009318-zt1o1bcz	148	48	incorporating	incorporate	VBG
cord-009318-zt1o1bcz	148	49	Forward	Forward	NNP
cord-009318-zt1o1bcz	148	50	Inner	Inner	NNP
cord-009318-zt1o1bcz	148	51	Primer	Primer	NNP
cord-009318-zt1o1bcz	148	52	(	(	-LRB-
cord-009318-zt1o1bcz	148	53	FIP	FIP	NNP
cord-009318-zt1o1bcz	148	54	)	)	-RRB-
cord-009318-zt1o1bcz	148	55	.	.	.
cord-009318-zt1o1bcz	149	1	Through	through	IN
cord-009318-zt1o1bcz	149	2	breathing	breathing	NN
cord-009318-zt1o1bcz	149	3	of	of	IN
cord-009318-zt1o1bcz	149	4	the	the	DT
cord-009318-zt1o1bcz	149	5	molecule	molecule	NN
cord-009318-zt1o1bcz	149	6	,	,	,
cord-009318-zt1o1bcz	149	7	the	the	DT
cord-009318-zt1o1bcz	149	8	3	3	CD
cord-009318-zt1o1bcz	149	9	of	of	IN
cord-009318-zt1o1bcz	149	10	one	one	CD
cord-009318-zt1o1bcz	149	11	strand	strand	NN
cord-009318-zt1o1bcz	149	12	may	may	MD
cord-009318-zt1o1bcz	149	13	form	form	VB
cord-009318-zt1o1bcz	149	14	a	a	DT
cord-009318-zt1o1bcz	149	15	second	second	JJ
cord-009318-zt1o1bcz	149	16	hairpin	hairpin	NN
cord-009318-zt1o1bcz	149	17	and	and	CC
cord-009318-zt1o1bcz	149	18	amplify	amplify	VB
cord-009318-zt1o1bcz	149	19	.	.	.
cord-009318-zt1o1bcz	150	1	Some	some	DT
cord-009318-zt1o1bcz	150	2	of	of	IN
cord-009318-zt1o1bcz	150	3	these	these	DT
cord-009318-zt1o1bcz	150	4	amplification	amplification	NN
cord-009318-zt1o1bcz	150	5	events	event	NNS
cord-009318-zt1o1bcz	150	6	incorporate	incorporate	VBP
cord-009318-zt1o1bcz	150	7	several	several	JJ
cord-009318-zt1o1bcz	150	8	random	random	JJ
cord-009318-zt1o1bcz	150	9	nucleotides	nucleotide	NNS
cord-009318-zt1o1bcz	150	10	via	via	IN
cord-009318-zt1o1bcz	150	11	terminal	terminal	JJ
cord-009318-zt1o1bcz	150	12	nucleotidyl	nucleotidyl	NNP
cord-009318-zt1o1bcz	150	13	transferase	transferase	NNP
cord-009318-zt1o1bcz	150	14	activity	activity	NN
cord-009318-zt1o1bcz	150	15	resulting	result	VBG
cord-009318-zt1o1bcz	150	16	in	in	IN
cord-009318-zt1o1bcz	150	17	a	a	DT
cord-009318-zt1o1bcz	150	18	pool	pool	NN
cord-009318-zt1o1bcz	150	19	of	of	IN
cord-009318-zt1o1bcz	150	20	hairpins	hairpin	NNS
cord-009318-zt1o1bcz	150	21	with	with	IN
cord-009318-zt1o1bcz	150	22	3	3	CD
cord-009318-zt1o1bcz	150	23	randomers	randomer	NNS
cord-009318-zt1o1bcz	150	24	.	.	.
cord-009318-zt1o1bcz	151	1	Sequences	sequence	NNS
cord-009318-zt1o1bcz	151	2	with	with	IN
cord-009318-zt1o1bcz	151	3	complementary	complementary	JJ
cord-009318-zt1o1bcz	151	4	randomers	randomer	NNS
cord-009318-zt1o1bcz	151	5	are	be	VBP
cord-009318-zt1o1bcz	151	6	selected	select	VBN
cord-009318-zt1o1bcz	151	7	in	in	IN
cord-009318-zt1o1bcz	151	8	vitro	vitro	FW
cord-009318-zt1o1bcz	151	9	to	to	TO
cord-009318-zt1o1bcz	151	10	amplify	amplify	VB
cord-009318-zt1o1bcz	151	11	.	.	.
cord-009318-zt1o1bcz	152	1	The	the	DT
cord-009318-zt1o1bcz	152	2	doublestranded	doublestranded	JJ
cord-009318-zt1o1bcz	152	3	product	product	NN
cord-009318-zt1o1bcz	152	4	of	of	IN
cord-009318-zt1o1bcz	152	5	this	this	DT
cord-009318-zt1o1bcz	152	6	amplification	amplification	NN
cord-009318-zt1o1bcz	152	7	can	can	MD
cord-009318-zt1o1bcz	152	8	,	,	,
cord-009318-zt1o1bcz	152	9	through	through	IN
cord-009318-zt1o1bcz	152	10	intramolecular	intramolecular	JJ
cord-009318-zt1o1bcz	152	11	hydrogen	hydrogen	NN
cord-009318-zt1o1bcz	152	12	bonding	bonding	NN
cord-009318-zt1o1bcz	152	13	,	,	,
cord-009318-zt1o1bcz	152	14	form	form	VB
cord-009318-zt1o1bcz	152	15	two	two	CD
cord-009318-zt1o1bcz	152	16	dumbbell	dumbbell	JJ
cord-009318-zt1o1bcz	152	17	-	-	HYPH
cord-009318-zt1o1bcz	152	18	like	like	JJ
cord-009318-zt1o1bcz	152	19	structures	structure	NNS
cord-009318-zt1o1bcz	152	20	and	and	CC
cord-009318-zt1o1bcz	152	21	amplify	amplify	VB
cord-009318-zt1o1bcz	152	22	in	in	IN
cord-009318-zt1o1bcz	152	23	a	a	DT
cord-009318-zt1o1bcz	152	24	fashion	fashion	NN
cord-009318-zt1o1bcz	152	25	similar	similar	JJ
cord-009318-zt1o1bcz	152	26	to	to	IN
cord-009318-zt1o1bcz	152	27	the	the	DT
cord-009318-zt1o1bcz	152	28	standard	standard	JJ
cord-009318-zt1o1bcz	152	29	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	152	30	mechanism	mechanism	NN
cord-009318-zt1o1bcz	152	31	,	,	,
cord-009318-zt1o1bcz	152	32	but	but	CC
cord-009318-zt1o1bcz	152	33	primed	prime	VBN
cord-009318-zt1o1bcz	152	34	by	by	IN
cord-009318-zt1o1bcz	152	35	BIP	BIP	NNP
cord-009318-zt1o1bcz	152	36	.	.	.
cord-009318-zt1o1bcz	153	1	Repetitive	repetitive	JJ
cord-009318-zt1o1bcz	153	2	cycles	cycle	NNS
cord-009318-zt1o1bcz	153	3	of	of	IN
cord-009318-zt1o1bcz	153	4	self	self	NN
cord-009318-zt1o1bcz	153	5	-	-	HYPH
cord-009318-zt1o1bcz	153	6	priming	priming	NN
cord-009318-zt1o1bcz	153	7	and	and	CC
cord-009318-zt1o1bcz	153	8	hairpin	hairpin	NN
cord-009318-zt1o1bcz	153	9	priming	priming	NN
cord-009318-zt1o1bcz	153	10	by	by	IN
cord-009318-zt1o1bcz	153	11	BIP	BIP	NNP
cord-009318-zt1o1bcz	153	12	result	result	NN
cord-009318-zt1o1bcz	153	13	in	in	IN
cord-009318-zt1o1bcz	153	14	numerous	numerous	JJ
cord-009318-zt1o1bcz	153	15	sequences	sequence	NNS
cord-009318-zt1o1bcz	153	16	with	with	IN
cord-009318-zt1o1bcz	153	17	complementarity	complementarity	NN
cord-009318-zt1o1bcz	153	18	and	and	CC
cord-009318-zt1o1bcz	153	19	the	the	DT
cord-009318-zt1o1bcz	153	20	possibility	possibility	NN
cord-009318-zt1o1bcz	153	21	of	of	IN
cord-009318-zt1o1bcz	153	22	multiple	multiple	JJ
cord-009318-zt1o1bcz	153	23	replication	replication	NN
cord-009318-zt1o1bcz	153	24	loci	locus	NNS
cord-009318-zt1o1bcz	153	25	within	within	IN
cord-009318-zt1o1bcz	153	26	a	a	DT
cord-009318-zt1o1bcz	153	27	single	single	JJ
cord-009318-zt1o1bcz	153	28	molecule	molecule	NN
cord-009318-zt1o1bcz	153	29	.	.	.
cord-009318-zt1o1bcz	154	1	This	this	DT
cord-009318-zt1o1bcz	154	2	process	process	NN
cord-009318-zt1o1bcz	154	3	can	can	MD
cord-009318-zt1o1bcz	154	4	give	give	VB
cord-009318-zt1o1bcz	154	5	rise	rise	NN
cord-009318-zt1o1bcz	154	6	to	to	IN
cord-009318-zt1o1bcz	154	7	very	very	RB
cord-009318-zt1o1bcz	154	8	long	long	JJ
cord-009318-zt1o1bcz	154	9	amplicons	amplicon	NNS
cord-009318-zt1o1bcz	154	10	,	,	,
cord-009318-zt1o1bcz	154	11	and	and	CC
cord-009318-zt1o1bcz	154	12	even	even	RB
cord-009318-zt1o1bcz	154	13	a	a	DT
cord-009318-zt1o1bcz	154	14	branched	branched	JJ
cord-009318-zt1o1bcz	154	15	,	,	,
cord-009318-zt1o1bcz	154	16	mesh	mesh	NN
cord-009318-zt1o1bcz	154	17	-	-	HYPH
cord-009318-zt1o1bcz	154	18	like	like	JJ
cord-009318-zt1o1bcz	154	19	network	network	NN
cord-009318-zt1o1bcz	154	20	from	from	IN
cord-009318-zt1o1bcz	154	21	the	the	DT
cord-009318-zt1o1bcz	154	22	multimeric	multimeric	NNP
cord-009318-zt1o1bcz	154	23	sequences	sequence	NNS
cord-009318-zt1o1bcz	154	24	annealing	anneal	VBG
cord-009318-zt1o1bcz	154	25	to	to	IN
cord-009318-zt1o1bcz	154	26	their	-PRON-	PRP$
cord-009318-zt1o1bcz	154	27	neighbors	neighbor	NNS
cord-009318-zt1o1bcz	154	28	or	or	CC
cord-009318-zt1o1bcz	154	29	in	in	IN
cord-009318-zt1o1bcz	154	30	a	a	DT
cord-009318-zt1o1bcz	154	31	self	self	NN
cord-009318-zt1o1bcz	154	32	-	-	HYPH
cord-009318-zt1o1bcz	154	33	complementary	complementary	JJ
cord-009318-zt1o1bcz	154	34	fashion	fashion	NN
cord-009318-zt1o1bcz	154	35	.	.	.
cord-009318-zt1o1bcz	155	1	A	a	DT
cord-009318-zt1o1bcz	155	2	simplified	simplified	JJ
cord-009318-zt1o1bcz	155	3	version	version	NN
cord-009318-zt1o1bcz	155	4	of	of	IN
cord-009318-zt1o1bcz	155	5	this	this	DT
cord-009318-zt1o1bcz	155	6	mechanism	mechanism	NN
cord-009318-zt1o1bcz	155	7	,	,	,
cord-009318-zt1o1bcz	155	8	annotated	annotate	VBN
cord-009318-zt1o1bcz	155	9	Figure	Figure	NNP
cord-009318-zt1o1bcz	155	10	2	2	CD
cord-009318-zt1o1bcz	155	11	.	.	.
cord-009318-zt1o1bcz	156	1	Quantification	quantification	NN
cord-009318-zt1o1bcz	156	2	of	of	IN
cord-009318-zt1o1bcz	156	3	junctions	junction	NNS
cord-009318-zt1o1bcz	156	4	using	use	VBG
cord-009318-zt1o1bcz	156	5	next	next	JJ
cord-009318-zt1o1bcz	156	6	-	-	HYPH
cord-009318-zt1o1bcz	156	7	generation	generation	NN
cord-009318-zt1o1bcz	156	8	sequencing	sequencing	NN
cord-009318-zt1o1bcz	156	9	of	of	IN
cord-009318-zt1o1bcz	156	10	select	select	JJ
cord-009318-zt1o1bcz	156	11	Chlamydia	Chlamydia	NNP
cord-009318-zt1o1bcz	156	12	trachomatis	trachomatis	NN
cord-009318-zt1o1bcz	156	13	amplification	amplification	NN
cord-009318-zt1o1bcz	156	14	products	product	NNS
cord-009318-zt1o1bcz	156	15	from	from	IN
cord-009318-zt1o1bcz	156	16	bulk	bulk	JJ
cord-009318-zt1o1bcz	156	17	reactions	reaction	NNS
cord-009318-zt1o1bcz	156	18	.	.	.
cord-009318-zt1o1bcz	157	1	Nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	157	2	amplification	amplification	NN
cord-009318-zt1o1bcz	157	3	from	from	IN
cord-009318-zt1o1bcz	157	4	the	the	DT
cord-009318-zt1o1bcz	157	5	no	no	DT
cord-009318-zt1o1bcz	157	6	-	-	HYPH
cord-009318-zt1o1bcz	157	7	template	template	NN
cord-009318-zt1o1bcz	157	8	control	control	NN
cord-009318-zt1o1bcz	157	9	using	use	VBG
cord-009318-zt1o1bcz	157	10	Bst	Bst	NNP
cord-009318-zt1o1bcz	157	11	3.0	3.0	NNP
cord-009318-zt1o1bcz	157	12	(	(	-LRB-
cord-009318-zt1o1bcz	157	13	A	a	NN
cord-009318-zt1o1bcz	157	14	)	)	-RRB-
cord-009318-zt1o1bcz	157	15	,	,	,
cord-009318-zt1o1bcz	157	16	including	include	VBG
cord-009318-zt1o1bcz	157	17	amplification	amplification	NN
cord-009318-zt1o1bcz	157	18	of	of	IN
cord-009318-zt1o1bcz	157	19	a	a	DT
cord-009318-zt1o1bcz	157	20	specific	specific	JJ
cord-009318-zt1o1bcz	157	21	target	target	NN
cord-009318-zt1o1bcz	157	22	contamination	contamination	NN
cord-009318-zt1o1bcz	157	23	(	(	-LRB-
cord-009318-zt1o1bcz	157	24	well	well	UH
cord-009318-zt1o1bcz	157	25	F8	F8	NNP
cord-009318-zt1o1bcz	157	26	)	)	-RRB-
cord-009318-zt1o1bcz	157	27	corresponding	correspond	VBG
cord-009318-zt1o1bcz	157	28	to	to	IN
cord-009318-zt1o1bcz	157	29	Figure	figure	NN
cord-009318-zt1o1bcz	157	30	1	1	CD
cord-009318-zt1o1bcz	157	31	K	K	NNP
cord-009318-zt1o1bcz	157	32	and	and	CC
cord-009318-zt1o1bcz	157	33	L.	L.	NNP
cord-009318-zt1o1bcz	157	34	Amplification	Amplification	NNP
cord-009318-zt1o1bcz	157	35	in	in	IN
cord-009318-zt1o1bcz	157	36	the	the	DT
cord-009318-zt1o1bcz	157	37	presence	presence	NN
cord-009318-zt1o1bcz	157	38	of	of	IN
cord-009318-zt1o1bcz	157	39	10	10	CD
cord-009318-zt1o1bcz	157	40	cp	cp	NN
cord-009318-zt1o1bcz	157	41	/	/	SYM
cord-009318-zt1o1bcz	157	42	l	l	NN
cord-009318-zt1o1bcz	157	43	template	template	NN
cord-009318-zt1o1bcz	157	44	(	(	-LRB-
cord-009318-zt1o1bcz	157	45	B	b	NN
cord-009318-zt1o1bcz	157	46	)	)	-RRB-
cord-009318-zt1o1bcz	157	47	,	,	,
cord-009318-zt1o1bcz	157	48	using	use	VBG
cord-009318-zt1o1bcz	157	49	Bst	Bst	NNP
cord-009318-zt1o1bcz	157	50	2.0	2.0	NNP
cord-009318-zt1o1bcz	157	51	(	(	-LRB-
cord-009318-zt1o1bcz	157	52	wells	wells	NNP
cord-009318-zt1o1bcz	157	53	A1-A3	A1-A3	NNP
cord-009318-zt1o1bcz	157	54	)	)	-RRB-
cord-009318-zt1o1bcz	157	55	corresponding	correspond	VBG
cord-009318-zt1o1bcz	157	56	to	to	IN
cord-009318-zt1o1bcz	157	57	Figure	Figure	NNP
cord-009318-zt1o1bcz	157	58	1A	1a	NN
cord-009318-zt1o1bcz	157	59	and	and	CC
cord-009318-zt1o1bcz	157	60	B	b	NN
cord-009318-zt1o1bcz	157	61	,	,	,
cord-009318-zt1o1bcz	157	62	and	and	CC
cord-009318-zt1o1bcz	157	63	Bst	Bst	NNP
cord-009318-zt1o1bcz	157	64	3.0	3.0	CD
cord-009318-zt1o1bcz	158	1	(	(	-LRB-
cord-009318-zt1o1bcz	158	2	wells	wells	NNP
cord-009318-zt1o1bcz	158	3	C1-C3	C1-C3	NNP
cord-009318-zt1o1bcz	158	4	)	)	-RRB-
cord-009318-zt1o1bcz	158	5	corresponding	correspond	VBG
cord-009318-zt1o1bcz	158	6	to	to	IN
cord-009318-zt1o1bcz	158	7	Figure	figure	NN
cord-009318-zt1o1bcz	158	8	1C	1c	NN
cord-009318-zt1o1bcz	158	9	and	and	CC
cord-009318-zt1o1bcz	158	10	D.	D.	NNP
cord-009318-zt1o1bcz	158	11	Non	Non	NNP
cord-009318-zt1o1bcz	158	12	-	-	JJ
cord-009318-zt1o1bcz	158	13	specific	specific	JJ
cord-009318-zt1o1bcz	158	14	amplification	amplification	NN
cord-009318-zt1o1bcz	158	15	in	in	IN
cord-009318-zt1o1bcz	158	16	the	the	DT
cord-009318-zt1o1bcz	158	17	presence	presence	NN
cord-009318-zt1o1bcz	158	18	of	of	IN
cord-009318-zt1o1bcz	158	19	10	10	CD
cord-009318-zt1o1bcz	158	20	cp	cp	NN
cord-009318-zt1o1bcz	158	21	/	/	SYM
cord-009318-zt1o1bcz	158	22	l	l	NN
cord-009318-zt1o1bcz	158	23	template	template	NN
cord-009318-zt1o1bcz	158	24	and	and	CC
cord-009318-zt1o1bcz	158	25	Bst	Bst	NNP
cord-009318-zt1o1bcz	158	26	3.0	3.0	NNP
cord-009318-zt1o1bcz	158	27	(	(	-LRB-
cord-009318-zt1o1bcz	158	28	well	well	RB
cord-009318-zt1o1bcz	158	29	C7	C7	NNP
cord-009318-zt1o1bcz	158	30	)	)	-RRB-
cord-009318-zt1o1bcz	158	31	corresponding	correspond	VBG
cord-009318-zt1o1bcz	158	32	to	to	IN
cord-009318-zt1o1bcz	158	33	Figure	figure	NN
cord-009318-zt1o1bcz	158	34	1C	1c	NN
cord-009318-zt1o1bcz	158	35	and	and	CC
cord-009318-zt1o1bcz	158	36	D.	D.	NNP
cord-009318-zt1o1bcz	159	1	For	for	IN
cord-009318-zt1o1bcz	159	2	a	a	DT
cord-009318-zt1o1bcz	159	3	complete	complete	JJ
cord-009318-zt1o1bcz	159	4	list	list	NN
cord-009318-zt1o1bcz	159	5	of	of	IN
cord-009318-zt1o1bcz	159	6	abbreviations	abbreviation	NNS
cord-009318-zt1o1bcz	159	7	used	use	VBN
cord-009318-zt1o1bcz	159	8	in	in	IN
cord-009318-zt1o1bcz	159	9	this	this	DT
cord-009318-zt1o1bcz	159	10	figure	figure	NN
cord-009318-zt1o1bcz	159	11	,	,	,
cord-009318-zt1o1bcz	159	12	see	see	VB
cord-009318-zt1o1bcz	159	13	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	159	14	Table	Table	NNP
cord-009318-zt1o1bcz	159	15	S2	s2	NN
cord-009318-zt1o1bcz	159	16	.	.	.
cord-009318-zt1o1bcz	160	1	Composite	composite	JJ
cord-009318-zt1o1bcz	160	2	image	image	NN
cord-009318-zt1o1bcz	160	3	of	of	IN
cord-009318-zt1o1bcz	160	4	select	select	JJ
cord-009318-zt1o1bcz	160	5	Chlamydia	Chlamydia	NNP
cord-009318-zt1o1bcz	160	6	trachomatis	trachomatis	NN
cord-009318-zt1o1bcz	160	7	amplification	amplification	NN
cord-009318-zt1o1bcz	160	8	products	product	NNS
cord-009318-zt1o1bcz	160	9	from	from	IN
cord-009318-zt1o1bcz	160	10	a	a	DT
cord-009318-zt1o1bcz	160	11	bulk	bulk	JJ
cord-009318-zt1o1bcz	160	12	reaction	reaction	NN
cord-009318-zt1o1bcz	160	13	.	.	.
cord-009318-zt1o1bcz	161	1	Products	product	NNS
cord-009318-zt1o1bcz	161	2	were	be	VBD
cord-009318-zt1o1bcz	161	3	collected	collect	VBN
cord-009318-zt1o1bcz	161	4	using	use	VBG
cord-009318-zt1o1bcz	161	5	D5000	D5000	NNP
cord-009318-zt1o1bcz	161	6	tape	tape	NN
cord-009318-zt1o1bcz	161	7	on	on	IN
cord-009318-zt1o1bcz	161	8	an	an	DT
cord-009318-zt1o1bcz	161	9	Agilent	Agilent	NNP
cord-009318-zt1o1bcz	161	10	TapeStation	TapeStation	NNP
cord-009318-zt1o1bcz	161	11	.	.	.
cord-009318-zt1o1bcz	162	1	Amplification	amplification	NN
cord-009318-zt1o1bcz	162	2	in	in	IN
cord-009318-zt1o1bcz	162	3	the	the	DT
cord-009318-zt1o1bcz	162	4	presence	presence	NN
cord-009318-zt1o1bcz	162	5	of	of	IN
cord-009318-zt1o1bcz	162	6	10	10	CD
cord-009318-zt1o1bcz	162	7	cp	cp	NN
cord-009318-zt1o1bcz	162	8	/	/	SYM
cord-009318-zt1o1bcz	162	9	l	l	NN
cord-009318-zt1o1bcz	162	10	template	template	NN
cord-009318-zt1o1bcz	162	11	using	use	VBG
cord-009318-zt1o1bcz	162	12	Bst	Bst	NNP
cord-009318-zt1o1bcz	162	13	2.0	2.0	NNP
cord-009318-zt1o1bcz	162	14	(	(	-LRB-
cord-009318-zt1o1bcz	162	15	lanes	lane	NNS
cord-009318-zt1o1bcz	162	16	A1-A3	A1-A3	NNP
cord-009318-zt1o1bcz	162	17	)	)	-RRB-
cord-009318-zt1o1bcz	162	18	corresponding	correspond	VBG
cord-009318-zt1o1bcz	162	19	to	to	IN
cord-009318-zt1o1bcz	162	20	Figure	Figure	NNP
cord-009318-zt1o1bcz	162	21	1A	1a	NN
cord-009318-zt1o1bcz	162	22	and	and	CC
cord-009318-zt1o1bcz	162	23	B	b	NN
cord-009318-zt1o1bcz	162	24	,	,	,
cord-009318-zt1o1bcz	162	25	and	and	CC
cord-009318-zt1o1bcz	162	26	Bst	Bst	NNP
cord-009318-zt1o1bcz	162	27	3.0	3.0	CD
cord-009318-zt1o1bcz	163	1	(	(	-LRB-
cord-009318-zt1o1bcz	163	2	lanes	lane	NNS
cord-009318-zt1o1bcz	163	3	C1-C3	C1-C3	NNP
cord-009318-zt1o1bcz	163	4	,	,	,
cord-009318-zt1o1bcz	163	5	C7	C7	NNP
cord-009318-zt1o1bcz	163	6	)	)	-RRB-
cord-009318-zt1o1bcz	163	7	corresponding	correspond	VBG
cord-009318-zt1o1bcz	163	8	to	to	IN
cord-009318-zt1o1bcz	163	9	Figure	figure	NN
cord-009318-zt1o1bcz	163	10	1C	1c	NN
cord-009318-zt1o1bcz	163	11	and	and	CC
cord-009318-zt1o1bcz	163	12	D.	D.	NNP
cord-009318-zt1o1bcz	163	13	Non	Non	NNP
cord-009318-zt1o1bcz	163	14	-	-	JJ
cord-009318-zt1o1bcz	163	15	specific	specific	JJ
cord-009318-zt1o1bcz	163	16	amplification	amplification	NN
cord-009318-zt1o1bcz	163	17	in	in	IN
cord-009318-zt1o1bcz	163	18	the	the	DT
cord-009318-zt1o1bcz	163	19	no	no	DT
cord-009318-zt1o1bcz	163	20	-	-	HYPH
cord-009318-zt1o1bcz	163	21	template	template	NN
cord-009318-zt1o1bcz	163	22	control	control	NN
cord-009318-zt1o1bcz	163	23	(	(	-LRB-
cord-009318-zt1o1bcz	163	24	NTC	NTC	NNP
cord-009318-zt1o1bcz	163	25	;	;	:
cord-009318-zt1o1bcz	163	26	lanes	lane	NNS
cord-009318-zt1o1bcz	163	27	E2-H1	E2-H1	NNP
cord-009318-zt1o1bcz	163	28	)	)	-RRB-
cord-009318-zt1o1bcz	163	29	correspond	correspond	VBP
cord-009318-zt1o1bcz	163	30	to	to	IN
cord-009318-zt1o1bcz	163	31	Figure	figure	NN
cord-009318-zt1o1bcz	163	32	1	1	CD
cord-009318-zt1o1bcz	163	33	K	K	NNP
cord-009318-zt1o1bcz	163	34	and	and	CC
cord-009318-zt1o1bcz	163	35	L.	L.	NNP
cord-009318-zt1o1bcz	163	36	Contrast	Contrast	NNP
cord-009318-zt1o1bcz	163	37	was	be	VBD
cord-009318-zt1o1bcz	163	38	determined	determine	VBN
cord-009318-zt1o1bcz	163	39	using	use	VBG
cord-009318-zt1o1bcz	163	40	the	the	DT
cord-009318-zt1o1bcz	163	41	automatic	automatic	JJ
cord-009318-zt1o1bcz	163	42	'	'	``
cord-009318-zt1o1bcz	163	43	scale	scale	NN
cord-009318-zt1o1bcz	163	44	to	to	TO
cord-009318-zt1o1bcz	163	45	sample	sample	VB
cord-009318-zt1o1bcz	163	46	'	'	''
cord-009318-zt1o1bcz	163	47	feature	feature	NN
cord-009318-zt1o1bcz	163	48	in	in	IN
cord-009318-zt1o1bcz	163	49	the	the	DT
cord-009318-zt1o1bcz	163	50	Agilent	Agilent	NNP
cord-009318-zt1o1bcz	163	51	TapeStation	TapeStation	NNP
cord-009318-zt1o1bcz	163	52	analysis	analysis	NN
cord-009318-zt1o1bcz	163	53	software	software	NN
cord-009318-zt1o1bcz	163	54	.	.	.
cord-009318-zt1o1bcz	164	1	with	with	IN
cord-009318-zt1o1bcz	164	2	sequencing	sequencing	NN
cord-009318-zt1o1bcz	164	3	data	datum	NNS
cord-009318-zt1o1bcz	164	4	,	,	,
cord-009318-zt1o1bcz	164	5	can	can	MD
cord-009318-zt1o1bcz	164	6	be	be	VB
cord-009318-zt1o1bcz	164	7	found	find	VBN
cord-009318-zt1o1bcz	164	8	in	in	IN
cord-009318-zt1o1bcz	164	9	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	164	10	Figure	Figure	NNP
cord-009318-zt1o1bcz	164	11	S3	s3	NN
cord-009318-zt1o1bcz	164	12	.	.	.
cord-009318-zt1o1bcz	165	1	In	in	IN
cord-009318-zt1o1bcz	165	2	more	more	JJR
cord-009318-zt1o1bcz	165	3	detail	detail	NN
cord-009318-zt1o1bcz	165	4	,	,	,
cord-009318-zt1o1bcz	165	5	a	a	DT
cord-009318-zt1o1bcz	165	6	potential	potential	JJ
cord-009318-zt1o1bcz	165	7	mechanism	mechanism	NN
cord-009318-zt1o1bcz	165	8	of	of	IN
cord-009318-zt1o1bcz	165	9	formation	formation	NN
cord-009318-zt1o1bcz	165	10	of	of	IN
cord-009318-zt1o1bcz	165	11	non	non	JJ
cord-009318-zt1o1bcz	165	12	-	-	JJ
cord-009318-zt1o1bcz	165	13	specific	specific	JJ
cord-009318-zt1o1bcz	165	14	products	product	NNS
cord-009318-zt1o1bcz	165	15	is	be	VBZ
cord-009318-zt1o1bcz	165	16	as	as	IN
cord-009318-zt1o1bcz	165	17	follows	follow	VBZ
cord-009318-zt1o1bcz	165	18	:	:	:
cord-009318-zt1o1bcz	165	19	initially	initially	RB
cord-009318-zt1o1bcz	165	20	,	,	,
cord-009318-zt1o1bcz	165	21	a	a	DT
cord-009318-zt1o1bcz	165	22	doublestranded	doublestranded	JJ
cord-009318-zt1o1bcz	165	23	amplicon	amplicon	NN
cord-009318-zt1o1bcz	165	24	is	be	VBZ
cord-009318-zt1o1bcz	165	25	generated	generate	VBN
cord-009318-zt1o1bcz	165	26	by	by	IN
cord-009318-zt1o1bcz	165	27	homo	homo	NN
cord-009318-zt1o1bcz	165	28	-	-	HYPH
cord-009318-zt1o1bcz	165	29	dimerization	dimerization	NN
cord-009318-zt1o1bcz	165	30	of	of	IN
cord-009318-zt1o1bcz	165	31	BIP	BIP	NNP
cord-009318-zt1o1bcz	165	32	,	,	,
cord-009318-zt1o1bcz	165	33	and	and	CC
cord-009318-zt1o1bcz	165	34	3	3	CD
cord-009318-zt1o1bcz	165	35	extension	extension	NN
cord-009318-zt1o1bcz	165	36	of	of	IN
cord-009318-zt1o1bcz	165	37	the	the	DT
cord-009318-zt1o1bcz	165	38	homodimer	homodimer	JJ
cord-009318-zt1o1bcz	165	39	to	to	TO
cord-009318-zt1o1bcz	165	40	produce	produce	VB
cord-009318-zt1o1bcz	165	41	a	a	DT
cord-009318-zt1o1bcz	165	42	partial	partial	JJ
cord-009318-zt1o1bcz	165	43	reverse	reverse	NN
cord-009318-zt1o1bcz	165	44	complement	complement	NN
cord-009318-zt1o1bcz	165	45	of	of	IN
cord-009318-zt1o1bcz	165	46	BIP	BIP	NNP
cord-009318-zt1o1bcz	165	47	(	(	-LRB-
cord-009318-zt1o1bcz	165	48	prcBIP	prcBIP	NNP
cord-009318-zt1o1bcz	165	49	)	)	-RRB-
cord-009318-zt1o1bcz	166	1	(	(	-LRB-
cord-009318-zt1o1bcz	166	2	Figure	figure	NN
cord-009318-zt1o1bcz	166	3	4	4	CD
cord-009318-zt1o1bcz	166	4	-	-	SYM
cord-009318-zt1o1bcz	166	5	1	1	CD
cord-009318-zt1o1bcz	166	6	)	)	-RRB-
cord-009318-zt1o1bcz	166	7	.	.	.
cord-009318-zt1o1bcz	167	1	Bst	Bst	NNP
cord-009318-zt1o1bcz	167	2	polymerase	polymerase	NN
cord-009318-zt1o1bcz	167	3	is	be	VBZ
cord-009318-zt1o1bcz	167	4	highly	highly	RB
cord-009318-zt1o1bcz	167	5	prone	prone	JJ
cord-009318-zt1o1bcz	167	6	to	to	IN
cord-009318-zt1o1bcz	167	7	mismatched	mismatched	JJ
cord-009318-zt1o1bcz	167	8	extension	extension	NN
cord-009318-zt1o1bcz	167	9	(	(	-LRB-
cord-009318-zt1o1bcz	167	10	36	36	CD
cord-009318-zt1o1bcz	167	11	)	)	-RRB-
cord-009318-zt1o1bcz	167	12	,	,	,
cord-009318-zt1o1bcz	167	13	and	and	CC
cord-009318-zt1o1bcz	167	14	the	the	DT
cord-009318-zt1o1bcz	167	15	two	two	CD
cord-009318-zt1o1bcz	167	16	base	base	NN
cord-009318-zt1o1bcz	167	17	pairs	pair	NNS
cord-009318-zt1o1bcz	167	18	of	of	IN
cord-009318-zt1o1bcz	167	19	CG	CG	NNP
cord-009318-zt1o1bcz	167	20	provide	provide	VBP
cord-009318-zt1o1bcz	167	21	a	a	DT
cord-009318-zt1o1bcz	167	22	sufficient	sufficient	JJ
cord-009318-zt1o1bcz	167	23	anchoring	anchoring	NN
cord-009318-zt1o1bcz	167	24	in	in	IN
cord-009318-zt1o1bcz	167	25	the	the	DT
cord-009318-zt1o1bcz	167	26	3	3	CD
cord-009318-zt1o1bcz	167	27	to	to	TO
cord-009318-zt1o1bcz	167	28	start	start	VB
cord-009318-zt1o1bcz	167	29	elongation	elongation	NN
cord-009318-zt1o1bcz	167	30	.	.	.
cord-009318-zt1o1bcz	168	1	Multiple	Multiple	NNP
cord-009318-zt1o1bcz	168	2	Primer	Primer	NNP
cord-009318-zt1o1bcz	168	3	Analyzer	Analyzer	NNP
cord-009318-zt1o1bcz	168	4	(	(	-LRB-
cord-009318-zt1o1bcz	168	5	ThermoFisher	ThermoFisher	NNP
cord-009318-zt1o1bcz	168	6	)	)	-RRB-
cord-009318-zt1o1bcz	168	7	does	do	VBZ
cord-009318-zt1o1bcz	168	8	not	not	RB
cord-009318-zt1o1bcz	168	9	identify	identify	VB
cord-009318-zt1o1bcz	168	10	the	the	DT
cord-009318-zt1o1bcz	168	11	BIP	BIP	NNP
cord-009318-zt1o1bcz	168	12	homodimer	homodimer	NN
cord-009318-zt1o1bcz	168	13	,	,	,
cord-009318-zt1o1bcz	168	14	unless	unless	IN
cord-009318-zt1o1bcz	168	15	maximum	maximum	JJ
cord-009318-zt1o1bcz	168	16	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	168	17	is	be	VBZ
cord-009318-zt1o1bcz	168	18	used	use	VBN
cord-009318-zt1o1bcz	168	19	.	.	.
cord-009318-zt1o1bcz	169	1	Alternatively	alternatively	RB
cord-009318-zt1o1bcz	169	2	,	,	,
cord-009318-zt1o1bcz	169	3	BIP	BIP	NNP
cord-009318-zt1o1bcz	169	4	-	-	HYPH
cord-009318-zt1o1bcz	169	5	prcBIP	prcBIP	NNP
cord-009318-zt1o1bcz	169	6	product	product	NN
cord-009318-zt1o1bcz	169	7	may	may	MD
cord-009318-zt1o1bcz	169	8	arise	arise	VB
cord-009318-zt1o1bcz	169	9	from	from	IN
cord-009318-zt1o1bcz	169	10	a	a	DT
cord-009318-zt1o1bcz	169	11	single	single	JJ
cord-009318-zt1o1bcz	169	12	stranded	strand	VBN
cord-009318-zt1o1bcz	169	13	BIP	bip	NN
cord-009318-zt1o1bcz	169	14	-	-	HYPH
cord-009318-zt1o1bcz	169	15	hairpin	hairpin	NNP
cord-009318-zt1o1bcz	169	16	,	,	,
cord-009318-zt1o1bcz	169	17	as	as	IN
cord-009318-zt1o1bcz	169	18	has	have	VBZ
cord-009318-zt1o1bcz	169	19	been	be	VBN
cord-009318-zt1o1bcz	169	20	observed	observe	VBN
cord-009318-zt1o1bcz	169	21	by	by	IN
cord-009318-zt1o1bcz	169	22	others	other	NNS
cord-009318-zt1o1bcz	169	23	(	(	-LRB-
cord-009318-zt1o1bcz	169	24	37	37	CD
cord-009318-zt1o1bcz	169	25	)	)	-RRB-
cord-009318-zt1o1bcz	169	26	,	,	,
cord-009318-zt1o1bcz	169	27	although	although	IN
cord-009318-zt1o1bcz	169	28	UNAfold	UNAfold	NNP
cord-009318-zt1o1bcz	169	29	(	(	-LRB-
cord-009318-zt1o1bcz	169	30	IDT	IDT	NNP
cord-009318-zt1o1bcz	169	31	)	)	-RRB-
cord-009318-zt1o1bcz	169	32	does	do	VBZ
cord-009318-zt1o1bcz	169	33	not	not	RB
cord-009318-zt1o1bcz	169	34	predict	predict	VB
cord-009318-zt1o1bcz	169	35	the	the	DT
cord-009318-zt1o1bcz	169	36	formation	formation	NN
cord-009318-zt1o1bcz	169	37	of	of	IN
cord-009318-zt1o1bcz	169	38	the	the	DT
cord-009318-zt1o1bcz	169	39	hairpin	hairpin	NN
cord-009318-zt1o1bcz	169	40	for	for	IN
cord-009318-zt1o1bcz	169	41	this	this	DT
cord-009318-zt1o1bcz	169	42	primer	primer	NN
cord-009318-zt1o1bcz	169	43	.	.	.
cord-009318-zt1o1bcz	170	1	These	these	DT
cord-009318-zt1o1bcz	170	2	structures	structure	NNS
cord-009318-zt1o1bcz	170	3	may	may	MD
cord-009318-zt1o1bcz	170	4	not	not	RB
cord-009318-zt1o1bcz	170	5	need	need	VB
cord-009318-zt1o1bcz	170	6	to	to	TO
cord-009318-zt1o1bcz	170	7	be	be	VB
cord-009318-zt1o1bcz	170	8	abundant	abundant	JJ
cord-009318-zt1o1bcz	170	9	at	at	IN
cord-009318-zt1o1bcz	170	10	equilibrium	equilibrium	NN
cord-009318-zt1o1bcz	170	11	,	,	,
cord-009318-zt1o1bcz	170	12	but	but	CC
cord-009318-zt1o1bcz	170	13	as	as	RB
cord-009318-zt1o1bcz	170	14	long	long	RB
cord-009318-zt1o1bcz	170	15	as	as	IN
cord-009318-zt1o1bcz	170	16	they	-PRON-	PRP
cord-009318-zt1o1bcz	170	17	are	be	VBP
cord-009318-zt1o1bcz	170	18	extended	extend	VBN
cord-009318-zt1o1bcz	170	19	by	by	IN
cord-009318-zt1o1bcz	170	20	the	the	DT
cord-009318-zt1o1bcz	170	21	polymerase	polymerase	NN
cord-009318-zt1o1bcz	170	22	,	,	,
cord-009318-zt1o1bcz	170	23	the	the	DT
cord-009318-zt1o1bcz	170	24	product	product	NN
cord-009318-zt1o1bcz	170	25	will	will	MD
cord-009318-zt1o1bcz	170	26	be	be	VB
cord-009318-zt1o1bcz	170	27	stabilized	stabilize	VBN
cord-009318-zt1o1bcz	170	28	and	and	CC
cord-009318-zt1o1bcz	170	29	will	will	MD
cord-009318-zt1o1bcz	170	30	accumulate	accumulate	VB
cord-009318-zt1o1bcz	170	31	.	.	.
cord-009318-zt1o1bcz	171	1	Upon	upon	IN
cord-009318-zt1o1bcz	171	2	accumulation	accumulation	NN
cord-009318-zt1o1bcz	171	3	of	of	IN
cord-009318-zt1o1bcz	171	4	the	the	DT
cord-009318-zt1o1bcz	171	5	BIP	BIP	NNP
cord-009318-zt1o1bcz	171	6	-	-	HYPH
cord-009318-zt1o1bcz	171	7	prcBIP	prcBIP	NNP
cord-009318-zt1o1bcz	171	8	construct	construct	NN
cord-009318-zt1o1bcz	171	9	,	,	,
cord-009318-zt1o1bcz	171	10	the	the	DT
cord-009318-zt1o1bcz	171	11	reverse	reverse	JJ
cord-009318-zt1o1bcz	171	12	complement	complement	NN
cord-009318-zt1o1bcz	171	13	of	of	IN
cord-009318-zt1o1bcz	171	14	FIP	fip	NN
cord-009318-zt1o1bcz	171	15	(	(	-LRB-
cord-009318-zt1o1bcz	171	16	rcFIP	rcFIP	NNS
cord-009318-zt1o1bcz	171	17	)	)	-RRB-
cord-009318-zt1o1bcz	171	18	is	be	VBZ
cord-009318-zt1o1bcz	171	19	incorporated	incorporate	VBN
cord-009318-zt1o1bcz	171	20	by	by	IN
cord-009318-zt1o1bcz	171	21	template	template	NN
cord-009318-zt1o1bcz	171	22	switching	switching	NN
cord-009318-zt1o1bcz	171	23	(	(	-LRB-
cord-009318-zt1o1bcz	171	24	Figure	figure	NN
cord-009318-zt1o1bcz	171	25	4	4	CD
cord-009318-zt1o1bcz	171	26	-2	-2	CD
cord-009318-zt1o1bcz	171	27	)	)	-RRB-
cord-009318-zt1o1bcz	171	28	.	.	.
cord-009318-zt1o1bcz	172	1	The	the	DT
cord-009318-zt1o1bcz	172	2	3	3	CD
cord-009318-zt1o1bcz	172	3	of	of	IN
cord-009318-zt1o1bcz	172	4	FIP	FIP	NNP
cord-009318-zt1o1bcz	172	5	is	be	VBZ
cord-009318-zt1o1bcz	172	6	within	within	IN
cord-009318-zt1o1bcz	172	7	spatial	spatial	JJ
cord-009318-zt1o1bcz	172	8	proximity	proximity	NN
cord-009318-zt1o1bcz	172	9	of	of	IN
cord-009318-zt1o1bcz	172	10	the	the	DT
cord-009318-zt1o1bcz	172	11	homo	homo	NN
cord-009318-zt1o1bcz	172	12	-	-	HYPH
cord-009318-zt1o1bcz	172	13	BIP	bip	NN
cord-009318-zt1o1bcz	172	14	sequence	sequence	NN
cord-009318-zt1o1bcz	172	15	due	due	IN
cord-009318-zt1o1bcz	172	16	to	to	IN
cord-009318-zt1o1bcz	172	17	microhomology	microhomology	NN
cord-009318-zt1o1bcz	172	18	of	of	IN
cord-009318-zt1o1bcz	172	19	to	to	TO
cord-009318-zt1o1bcz	172	20	5	5	CD
cord-009318-zt1o1bcz	172	21	end	end	NN
cord-009318-zt1o1bcz	172	22	of	of	IN
cord-009318-zt1o1bcz	172	23	the	the	DT
cord-009318-zt1o1bcz	172	24	double	double	RB
cord-009318-zt1o1bcz	172	25	-	-	HYPH
cord-009318-zt1o1bcz	172	26	stranded	strand	VBN
cord-009318-zt1o1bcz	172	27	sequence	sequence	NN
cord-009318-zt1o1bcz	172	28	coupled	couple	VBN
cord-009318-zt1o1bcz	172	29	with	with	IN
cord-009318-zt1o1bcz	172	30	rapid	rapid	JJ
cord-009318-zt1o1bcz	172	31	breathing	breathing	NN
cord-009318-zt1o1bcz	172	32	of	of	IN
cord-009318-zt1o1bcz	172	33	two	two	CD
cord-009318-zt1o1bcz	172	34	base	base	NN
cord-009318-zt1o1bcz	172	35	pairs	pair	NNS
cord-009318-zt1o1bcz	172	36	of	of	IN
cord-009318-zt1o1bcz	172	37	TA	TA	NNP
cord-009318-zt1o1bcz	172	38	.	.	.
cord-009318-zt1o1bcz	173	1	This	this	DT
cord-009318-zt1o1bcz	173	2	allows	allow	VBZ
cord-009318-zt1o1bcz	173	3	temporary	temporary	JJ
cord-009318-zt1o1bcz	173	4	insertion	insertion	NN
cord-009318-zt1o1bcz	173	5	and	and	CC
cord-009318-zt1o1bcz	173	6	hybridization	hybridization	NN
cord-009318-zt1o1bcz	173	7	of	of	IN
cord-009318-zt1o1bcz	173	8	FIP	FIP	NNP
cord-009318-zt1o1bcz	173	9	with	with	IN
cord-009318-zt1o1bcz	173	10	the	the	DT
cord-009318-zt1o1bcz	173	11	double	double	RB
cord-009318-zt1o1bcz	173	12	-	-	HYPH
cord-009318-zt1o1bcz	173	13	stranded	stranded	JJ
cord-009318-zt1o1bcz	173	14	BIP	BIP	NNP
cord-009318-zt1o1bcz	173	15	-	-	HYPH
cord-009318-zt1o1bcz	173	16	prcBIP	prcbip	NN
cord-009318-zt1o1bcz	173	17	sequence	sequence	NN
cord-009318-zt1o1bcz	173	18	(	(	-LRB-
cord-009318-zt1o1bcz	173	19	Figure	Figure	NNP
cord-009318-zt1o1bcz	173	20	4	4	CD
cord-009318-zt1o1bcz	173	21	-	-	SYM
cord-009318-zt1o1bcz	173	22	3	3	CD
cord-009318-zt1o1bcz	173	23	)	)	-RRB-
cord-009318-zt1o1bcz	173	24	.	.	.
cord-009318-zt1o1bcz	174	1	When	when	WRB
cord-009318-zt1o1bcz	174	2	the	the	DT
cord-009318-zt1o1bcz	174	3	polymerase	polymerase	NN
cord-009318-zt1o1bcz	174	4	is	be	VBZ
cord-009318-zt1o1bcz	174	5	also	also	RB
cord-009318-zt1o1bcz	174	6	in	in	IN
cord-009318-zt1o1bcz	174	7	proximity	proximity	NN
cord-009318-zt1o1bcz	174	8	of	of	IN
cord-009318-zt1o1bcz	174	9	this	this	DT
cord-009318-zt1o1bcz	174	10	reaction	reaction	NN
cord-009318-zt1o1bcz	174	11	,	,	,
cord-009318-zt1o1bcz	174	12	FIP	FIP	NNP
cord-009318-zt1o1bcz	174	13	slips	slip	VBZ
cord-009318-zt1o1bcz	174	14	out	out	IN
cord-009318-zt1o1bcz	174	15	of	of	IN
cord-009318-zt1o1bcz	174	16	the	the	DT
cord-009318-zt1o1bcz	174	17	junction	junction	NN
cord-009318-zt1o1bcz	174	18	,	,	,
cord-009318-zt1o1bcz	174	19	and	and	CC
cord-009318-zt1o1bcz	174	20	the	the	DT
cord-009318-zt1o1bcz	174	21	polymerase	polymerase	NN
cord-009318-zt1o1bcz	174	22	elongates	elongate	VBZ
cord-009318-zt1o1bcz	174	23	across	across	IN
cord-009318-zt1o1bcz	174	24	the	the	DT
cord-009318-zt1o1bcz	174	25	3	3	CD
cord-009318-zt1o1bcz	174	26	discontinuous	discontinuous	JJ
cord-009318-zt1o1bcz	174	27	junction	junction	NN
cord-009318-zt1o1bcz	174	28	(	(	-LRB-
cord-009318-zt1o1bcz	174	29	33,35	33,35	CD
cord-009318-zt1o1bcz	174	30	)	)	-RRB-
cord-009318-zt1o1bcz	174	31	templated	template	VBN
cord-009318-zt1o1bcz	174	32	by	by	IN
cord-009318-zt1o1bcz	174	33	FIP	FIP	NNP
cord-009318-zt1o1bcz	174	34	(	(	-LRB-
cord-009318-zt1o1bcz	174	35	Figure	Figure	NNP
cord-009318-zt1o1bcz	174	36	4	4	CD
cord-009318-zt1o1bcz	174	37	-	-	SYM
cord-009318-zt1o1bcz	174	38	4	4	CD
cord-009318-zt1o1bcz	174	39	)	)	-RRB-
cord-009318-zt1o1bcz	174	40	.	.	.
cord-009318-zt1o1bcz	175	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	175	2	confirmed	confirm	VBD
cord-009318-zt1o1bcz	175	3	the	the	DT
cord-009318-zt1o1bcz	175	4	interaction	interaction	NN
cord-009318-zt1o1bcz	175	5	of	of	IN
cord-009318-zt1o1bcz	175	6	FIP	FIP	NNP
cord-009318-zt1o1bcz	175	7	and	and	CC
cord-009318-zt1o1bcz	175	8	BIP	BIP	NNP
cord-009318-zt1o1bcz	175	9	produced	produce	VBD
cord-009318-zt1o1bcz	175	10	the	the	DT
cord-009318-zt1o1bcz	175	11	high	high	JJ
cord-009318-zt1o1bcz	175	12	-	-	HYPH
cord-009318-zt1o1bcz	175	13	T	t	NN
cord-009318-zt1o1bcz	175	14	m	m	NN
cord-009318-zt1o1bcz	175	15	non	non	JJ
cord-009318-zt1o1bcz	175	16	-	-	JJ
cord-009318-zt1o1bcz	175	17	specific	specific	JJ
cord-009318-zt1o1bcz	175	18	amplification	amplification	NN
cord-009318-zt1o1bcz	175	19	,	,	,
cord-009318-zt1o1bcz	175	20	and	and	CC
cord-009318-zt1o1bcz	175	21	that	that	DT
cord-009318-zt1o1bcz	175	22	elimination	elimination	NN
cord-009318-zt1o1bcz	175	23	of	of	IN
cord-009318-zt1o1bcz	175	24	3	3	CD
cord-009318-zt1o1bcz	175	25	microhomology	microhomology	NN
cord-009318-zt1o1bcz	175	26	could	could	MD
cord-009318-zt1o1bcz	175	27	significantly	significantly	RB
cord-009318-zt1o1bcz	175	28	reduce	reduce	VB
cord-009318-zt1o1bcz	175	29	high	high	JJ
cord-009318-zt1o1bcz	175	30	-	-	HYPH
cord-009318-zt1o1bcz	175	31	T	t	NN
cord-009318-zt1o1bcz	175	32	m	m	NN
cord-009318-zt1o1bcz	175	33	non	non	JJ
cord-009318-zt1o1bcz	175	34	-	-	JJ
cord-009318-zt1o1bcz	175	35	specific	specific	JJ
cord-009318-zt1o1bcz	175	36	amplification	amplification	NN
cord-009318-zt1o1bcz	175	37	(	(	-LRB-
cord-009318-zt1o1bcz	175	38	Supplementary	supplementary	JJ
cord-009318-zt1o1bcz	175	39	 	 	_SP
cord-009318-zt1o1bcz	175	40	Figures	figure	NNS
cord-009318-zt1o1bcz	175	41	S4	S4	NNP
cord-009318-zt1o1bcz	175	42	-	-	HYPH
cord-009318-zt1o1bcz	175	43	6	6	CD
cord-009318-zt1o1bcz	175	44	and	and	CC
cord-009318-zt1o1bcz	175	45	Tables	Tables	NNP
cord-009318-zt1o1bcz	175	46	S3	S3	NNP
cord-009318-zt1o1bcz	175	47	-	-	HYPH
cord-009318-zt1o1bcz	175	48	4	4	CD
cord-009318-zt1o1bcz	175	49	)	)	-RRB-
cord-009318-zt1o1bcz	175	50	.	.	.
cord-009318-zt1o1bcz	176	1	After	after	IN
cord-009318-zt1o1bcz	176	2	elongation	elongation	NN
cord-009318-zt1o1bcz	176	3	,	,	,
cord-009318-zt1o1bcz	176	4	the	the	DT
cord-009318-zt1o1bcz	176	5	FIP	fip	NN
cord-009318-zt1o1bcz	176	6	which	which	WDT
cord-009318-zt1o1bcz	176	7	has	have	VBZ
cord-009318-zt1o1bcz	176	8	served	serve	VBN
cord-009318-zt1o1bcz	176	9	as	as	IN
cord-009318-zt1o1bcz	176	10	template	template	NN
cord-009318-zt1o1bcz	176	11	,	,	,
cord-009318-zt1o1bcz	176	12	is	be	VBZ
cord-009318-zt1o1bcz	176	13	poised	poise	VBN
cord-009318-zt1o1bcz	176	14	to	to	TO
cord-009318-zt1o1bcz	176	15	prime	prime	VB
cord-009318-zt1o1bcz	176	16	in	in	IN
cord-009318-zt1o1bcz	176	17	the	the	DT
cord-009318-zt1o1bcz	176	18	opposite	opposite	JJ
cord-009318-zt1o1bcz	176	19	direction	direction	NN
cord-009318-zt1o1bcz	176	20	(	(	-LRB-
cord-009318-zt1o1bcz	176	21	Figure	figure	NN
cord-009318-zt1o1bcz	176	22	4	4	CD
cord-009318-zt1o1bcz	176	23	-5	-5	CD
cord-009318-zt1o1bcz	176	24	)	)	-RRB-
cord-009318-zt1o1bcz	176	25	.	.	.
cord-009318-zt1o1bcz	177	1	This	this	DT
cord-009318-zt1o1bcz	177	2	either	either	DT
cord-009318-zt1o1bcz	177	3	displaces	displace	VBZ
cord-009318-zt1o1bcz	177	4	the	the	DT
cord-009318-zt1o1bcz	177	5	initial	initial	JJ
cord-009318-zt1o1bcz	177	6	BIP	bip	NN
cord-009318-zt1o1bcz	177	7	mispairing	mispaire	VBG
cord-009318-zt1o1bcz	177	8	(	(	-LRB-
cord-009318-zt1o1bcz	177	9	BIP	BIP	NNP
cord-009318-zt1o1bcz	177	10	*	*	NFP
cord-009318-zt1o1bcz	177	11	)	)	-RRB-
cord-009318-zt1o1bcz	177	12	or	or	CC
cord-009318-zt1o1bcz	177	13	opens	open	VBZ
cord-009318-zt1o1bcz	177	14	the	the	DT
cord-009318-zt1o1bcz	177	15	hairpin	hairpin	NN
cord-009318-zt1o1bcz	177	16	,	,	,
cord-009318-zt1o1bcz	177	17	resulting	result	VBG
cord-009318-zt1o1bcz	177	18	in	in	IN
cord-009318-zt1o1bcz	177	19	a	a	DT
cord-009318-zt1o1bcz	177	20	double	double	RB
cord-009318-zt1o1bcz	177	21	-	-	HYPH
cord-009318-zt1o1bcz	177	22	stranded	stranded	JJ
cord-009318-zt1o1bcz	177	23	BIP	BIP	NNP
cord-009318-zt1o1bcz	177	24	-	-	HYPH
cord-009318-zt1o1bcz	177	25	prcBIP	prcBIP	NNP
cord-009318-zt1o1bcz	177	26	-	-	HYPH
cord-009318-zt1o1bcz	177	27	FIP	fip	NN
cord-009318-zt1o1bcz	177	28	product	product	NN
cord-009318-zt1o1bcz	177	29	(	(	-LRB-
cord-009318-zt1o1bcz	177	30	Figure	Figure	NNP
cord-009318-zt1o1bcz	177	31	4	4	CD
cord-009318-zt1o1bcz	177	32	-	-	SYM
cord-009318-zt1o1bcz	177	33	6	6	CD
cord-009318-zt1o1bcz	177	34	)	)	-RRB-
cord-009318-zt1o1bcz	177	35	.	.	.
cord-009318-zt1o1bcz	178	1	This	this	DT
cord-009318-zt1o1bcz	178	2	three	three	CD
cord-009318-zt1o1bcz	178	3	part	part	NN
cord-009318-zt1o1bcz	178	4	junction	junction	NN
cord-009318-zt1o1bcz	178	5	is	be	VBZ
cord-009318-zt1o1bcz	178	6	observed	observe	VBN
cord-009318-zt1o1bcz	178	7	as	as	IN
cord-009318-zt1o1bcz	178	8	a	a	DT
cord-009318-zt1o1bcz	178	9	complete	complete	JJ
cord-009318-zt1o1bcz	178	10	product	product	NN
cord-009318-zt1o1bcz	178	11	in	in	IN
cord-009318-zt1o1bcz	178	12	NGS	NGS	NNP
cord-009318-zt1o1bcz	178	13	data	datum	NNS
cord-009318-zt1o1bcz	178	14	.	.	.
cord-009318-zt1o1bcz	179	1	Breathing	breathing	NN
cord-009318-zt1o1bcz	179	2	of	of	IN
cord-009318-zt1o1bcz	179	3	double	double	RB
cord-009318-zt1o1bcz	179	4	-	-	HYPH
cord-009318-zt1o1bcz	179	5	stranded	stranded	JJ
cord-009318-zt1o1bcz	179	6	BIP	BIP	NNP
cord-009318-zt1o1bcz	179	7	-	-	HYPH
cord-009318-zt1o1bcz	179	8	prcBIP	prcBIP	NNP
cord-009318-zt1o1bcz	179	9	-	-	HYPH
cord-009318-zt1o1bcz	179	10	FIP	FIP	NNP
cord-009318-zt1o1bcz	179	11	is	be	VBZ
cord-009318-zt1o1bcz	179	12	prone	prone	JJ
cord-009318-zt1o1bcz	179	13	to	to	IN
cord-009318-zt1o1bcz	179	14	formation	formation	NN
cord-009318-zt1o1bcz	179	15	of	of	IN
cord-009318-zt1o1bcz	179	16	an	an	DT
cord-009318-zt1o1bcz	179	17	intramolecular	intramolecular	JJ
cord-009318-zt1o1bcz	179	18	self	self	NN
cord-009318-zt1o1bcz	179	19	-	-	HYPH
cord-009318-zt1o1bcz	179	20	priming	prime	VBG
cord-009318-zt1o1bcz	179	21	hairpin	hairpin	NN
cord-009318-zt1o1bcz	179	22	of	of	IN
cord-009318-zt1o1bcz	180	1	rcBIP	rcbip	RB
cord-009318-zt1o1bcz	180	2	-	-	HYPH
cord-009318-zt1o1bcz	180	3	pBIP	pbip	JJ
cord-009318-zt1o1bcz	180	4	(	(	-LRB-
cord-009318-zt1o1bcz	180	5	Figure	figure	NN
cord-009318-zt1o1bcz	180	6	4	4	CD
cord-009318-zt1o1bcz	180	7	.	.	.
cord-009318-zt1o1bcz	181	1	With	with	IN
cord-009318-zt1o1bcz	181	2	each	each	DT
cord-009318-zt1o1bcz	181	3	amplification	amplification	NN
cord-009318-zt1o1bcz	181	4	,	,	,
cord-009318-zt1o1bcz	181	5	and	and	CC
cord-009318-zt1o1bcz	181	6	re	re	NN
cord-009318-zt1o1bcz	181	7	-	-	NN
cord-009318-zt1o1bcz	181	8	prime	prime	NN
cord-009318-zt1o1bcz	181	9	by	by	IN
cord-009318-zt1o1bcz	181	10	FIP	FIP	NNP
cord-009318-zt1o1bcz	181	11	,	,	,
cord-009318-zt1o1bcz	181	12	a	a	DT
cord-009318-zt1o1bcz	181	13	single	single	JJ
cord-009318-zt1o1bcz	181	14	product	product	NN
cord-009318-zt1o1bcz	181	15	is	be	VBZ
cord-009318-zt1o1bcz	181	16	generated	generate	VBN
cord-009318-zt1o1bcz	181	17	.	.	.
cord-009318-zt1o1bcz	182	1	This	this	DT
cord-009318-zt1o1bcz	182	2	process	process	NN
cord-009318-zt1o1bcz	182	3	of	of	IN
cord-009318-zt1o1bcz	182	4	hairpin	hairpin	JJ
cord-009318-zt1o1bcz	182	5	accumulation	accumulation	NN
cord-009318-zt1o1bcz	182	6	would	would	MD
cord-009318-zt1o1bcz	182	7	cause	cause	VB
cord-009318-zt1o1bcz	182	8	the	the	DT
cord-009318-zt1o1bcz	182	9	linear	linear	NN
cord-009318-zt1o1bcz	182	10	'	'	``
cord-009318-zt1o1bcz	182	11	rinsing	rinse	VBG
cord-009318-zt1o1bcz	182	12	'	'	''
cord-009318-zt1o1bcz	182	13	baseline	baseline	NN
cord-009318-zt1o1bcz	182	14	observed	observe	VBN
cord-009318-zt1o1bcz	182	15	by	by	IN
cord-009318-zt1o1bcz	182	16	other	other	JJ
cord-009318-zt1o1bcz	182	17	researchers	researcher	NNS
cord-009318-zt1o1bcz	182	18	(	(	-LRB-
cord-009318-zt1o1bcz	182	19	37	37	CD
cord-009318-zt1o1bcz	182	20	)	)	-RRB-
cord-009318-zt1o1bcz	182	21	.	.	.
cord-009318-zt1o1bcz	183	1	Within	within	IN
cord-009318-zt1o1bcz	183	2	this	this	DT
cord-009318-zt1o1bcz	183	3	pool	pool	NN
cord-009318-zt1o1bcz	183	4	of	of	IN
cord-009318-zt1o1bcz	183	5	linear	linear	JJ
cord-009318-zt1o1bcz	183	6	amplifying	amplify	VBG
cord-009318-zt1o1bcz	183	7	products	product	NNS
cord-009318-zt1o1bcz	183	8	,	,	,
cord-009318-zt1o1bcz	183	9	the	the	DT
cord-009318-zt1o1bcz	183	10	Bst	Bst	NNP
cord-009318-zt1o1bcz	183	11	enzyme	enzyme	NN
cord-009318-zt1o1bcz	183	12	will	will	MD
cord-009318-zt1o1bcz	183	13	randomly	randomly	RB
cord-009318-zt1o1bcz	183	14	incorporate	incorporate	VB
cord-009318-zt1o1bcz	183	15	additional	additional	JJ
cord-009318-zt1o1bcz	183	16	nucleotides	nucleotide	NNS
cord-009318-zt1o1bcz	183	17	at	at	IN
cord-009318-zt1o1bcz	183	18	the	the	DT
cord-009318-zt1o1bcz	183	19	3	3	CD
cord-009318-zt1o1bcz	183	20	end	end	NN
cord-009318-zt1o1bcz	183	21	of	of	IN
cord-009318-zt1o1bcz	183	22	FIP	fip	NN
cord-009318-zt1o1bcz	183	23	-	-	HYPH
cord-009318-zt1o1bcz	183	24	pBIP	pbip	NN
cord-009318-zt1o1bcz	183	25	-	-	HYPH
cord-009318-zt1o1bcz	183	26	rcBIP	rcbip	RB
cord-009318-zt1o1bcz	183	27	-	-	HYPH
cord-009318-zt1o1bcz	183	28	rcFIP	rcfip	NN
cord-009318-zt1o1bcz	183	29	via	via	IN
cord-009318-zt1o1bcz	183	30	terminal	terminal	JJ
cord-009318-zt1o1bcz	183	31	transferase	transferase	NN
cord-009318-zt1o1bcz	183	32	activity	activity	NN
cord-009318-zt1o1bcz	183	33	(	(	-LRB-
cord-009318-zt1o1bcz	183	34	Figure	Figure	NNP
cord-009318-zt1o1bcz	183	35	4	4	CD
cord-009318-zt1o1bcz	183	36	-11	-11	''
cord-009318-zt1o1bcz	183	37	)	)	-RRB-
cord-009318-zt1o1bcz	183	38	.	.	.
cord-009318-zt1o1bcz	184	1	Our	-PRON-	PRP$
cord-009318-zt1o1bcz	184	2	sequencing	sequencing	NN
cord-009318-zt1o1bcz	184	3	methods	method	NNS
cord-009318-zt1o1bcz	184	4	are	be	VBP
cord-009318-zt1o1bcz	184	5	unable	unable	JJ
cord-009318-zt1o1bcz	184	6	to	to	TO
cord-009318-zt1o1bcz	184	7	observe	observe	VB
cord-009318-zt1o1bcz	184	8	a	a	DT
cord-009318-zt1o1bcz	184	9	FIP	fip	NN
cord-009318-zt1o1bcz	184	10	-	-	HYPH
cord-009318-zt1o1bcz	184	11	randomer	randomer	NN
cord-009318-zt1o1bcz	184	12	hairpin	hairpin	NN
cord-009318-zt1o1bcz	184	13	because	because	IN
cord-009318-zt1o1bcz	184	14	adapter	adapter	NN
cord-009318-zt1o1bcz	184	15	ligation	ligation	NN
cord-009318-zt1o1bcz	184	16	requires	require	VBZ
cord-009318-zt1o1bcz	184	17	double	double	RB
cord-009318-zt1o1bcz	184	18	-	-	HYPH
cord-009318-zt1o1bcz	184	19	stranded	strand	VBN
cord-009318-zt1o1bcz	184	20	products	product	NNS
cord-009318-zt1o1bcz	184	21	.	.	.
cord-009318-zt1o1bcz	185	1	This	this	DT
cord-009318-zt1o1bcz	185	2	pool	pool	NN
cord-009318-zt1o1bcz	185	3	of	of	IN
cord-009318-zt1o1bcz	185	4	hairpins	hairpin	NNS
cord-009318-zt1o1bcz	185	5	with	with	IN
cord-009318-zt1o1bcz	185	6	random	random	JJ
cord-009318-zt1o1bcz	185	7	sequences	sequence	NNS
cord-009318-zt1o1bcz	185	8	will	will	MD
cord-009318-zt1o1bcz	185	9	accumulate	accumulate	VB
cord-009318-zt1o1bcz	185	10	until	until	IN
cord-009318-zt1o1bcz	185	11	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	185	12	selects	select	VBZ
cord-009318-zt1o1bcz	185	13	for	for	IN
cord-009318-zt1o1bcz	185	14	sequences	sequence	NNS
cord-009318-zt1o1bcz	185	15	that	that	WDT
cord-009318-zt1o1bcz	185	16	amplify	amplify	VBP
cord-009318-zt1o1bcz	185	17	by	by	IN
cord-009318-zt1o1bcz	185	18	sharing	share	VBG
cord-009318-zt1o1bcz	185	19	complementary	complementary	JJ
cord-009318-zt1o1bcz	185	20	3	3	CD
cord-009318-zt1o1bcz	185	21	'	'	POS
cord-009318-zt1o1bcz	185	22	toe	toe	NN
cord-009318-zt1o1bcz	185	23	holds	hold	VBZ
cord-009318-zt1o1bcz	185	24	'	'	''
cord-009318-zt1o1bcz	186	1	(	(	-LRB-
cord-009318-zt1o1bcz	186	2	Figure	figure	NN
cord-009318-zt1o1bcz	186	3	4	4	CD
cord-009318-zt1o1bcz	186	4	-12	-12	CD
cord-009318-zt1o1bcz	186	5	)	)	-RRB-
cord-009318-zt1o1bcz	186	6	.	.	.
cord-009318-zt1o1bcz	187	1	Much	much	RB
cord-009318-zt1o1bcz	187	2	like	like	IN
cord-009318-zt1o1bcz	187	3	in	in	FW
cord-009318-zt1o1bcz	187	4	vitro	vitro	FW
cord-009318-zt1o1bcz	187	5	evolution	evolution	NN
cord-009318-zt1o1bcz	187	6	,	,	,
cord-009318-zt1o1bcz	187	7	those	those	DT
cord-009318-zt1o1bcz	187	8	sequences	sequence	NNS
cord-009318-zt1o1bcz	187	9	with	with	IN
cord-009318-zt1o1bcz	187	10	the	the	DT
cord-009318-zt1o1bcz	187	11	highest	high	JJS
cord-009318-zt1o1bcz	187	12	probability	probability	NN
cord-009318-zt1o1bcz	187	13	of	of	IN
cord-009318-zt1o1bcz	187	14	amplification	amplification	NN
cord-009318-zt1o1bcz	187	15	are	be	VBP
cord-009318-zt1o1bcz	187	16	selected	select	VBN
cord-009318-zt1o1bcz	187	17	(	(	-LRB-
cord-009318-zt1o1bcz	187	18	32	32	CD
cord-009318-zt1o1bcz	187	19	)	)	-RRB-
cord-009318-zt1o1bcz	187	20	.	.	.
cord-009318-zt1o1bcz	188	1	The	the	DT
cord-009318-zt1o1bcz	188	2	lack	lack	NN
cord-009318-zt1o1bcz	188	3	of	of	IN
cord-009318-zt1o1bcz	188	4	a	a	DT
cord-009318-zt1o1bcz	188	5	thermal	thermal	JJ
cord-009318-zt1o1bcz	188	6	gating	gating	NN
cord-009318-zt1o1bcz	188	7	mechanism	mechanism	NN
cord-009318-zt1o1bcz	188	8	in	in	IN
cord-009318-zt1o1bcz	188	9	LAMP	lamp	NN
cord-009318-zt1o1bcz	188	10	and	and	CC
cord-009318-zt1o1bcz	188	11	lack	lack	NN
cord-009318-zt1o1bcz	188	12	of	of	IN
cord-009318-zt1o1bcz	188	13	3	3	CD
cord-009318-zt1o1bcz	188	14	-5	-5	NN
cord-009318-zt1o1bcz	188	15	exonuclease	exonuclease	NN
cord-009318-zt1o1bcz	188	16	activity	activity	NN
cord-009318-zt1o1bcz	188	17	makes	make	VBZ
cord-009318-zt1o1bcz	188	18	the	the	DT
cord-009318-zt1o1bcz	188	19	amplification	amplification	NN
cord-009318-zt1o1bcz	188	20	reaction	reaction	NN
cord-009318-zt1o1bcz	188	21	especially	especially	RB
cord-009318-zt1o1bcz	188	22	prone	prone	JJ
cord-009318-zt1o1bcz	188	23	to	to	IN
cord-009318-zt1o1bcz	188	24	in	in	FW
cord-009318-zt1o1bcz	188	25	vitro	vitro	FW
cord-009318-zt1o1bcz	188	26	evolution	evolution	NN
cord-009318-zt1o1bcz	188	27	of	of	IN
cord-009318-zt1o1bcz	188	28	self	self	NN
cord-009318-zt1o1bcz	188	29	-	-	HYPH
cord-009318-zt1o1bcz	188	30	amplifying	amplify	VBG
cord-009318-zt1o1bcz	188	31	products	product	NNS
cord-009318-zt1o1bcz	188	32	.	.	.
cord-009318-zt1o1bcz	189	1	When	when	WRB
cord-009318-zt1o1bcz	189	2	considered	consider	VBN
cord-009318-zt1o1bcz	189	3	in	in	IN
cord-009318-zt1o1bcz	189	4	this	this	DT
cord-009318-zt1o1bcz	189	5	light	light	NN
cord-009318-zt1o1bcz	189	6	,	,	,
cord-009318-zt1o1bcz	189	7	it	-PRON-	PRP
cord-009318-zt1o1bcz	189	8	is	be	VBZ
cord-009318-zt1o1bcz	189	9	unsurprising	unsurprising	JJ
cord-009318-zt1o1bcz	189	10	that	that	IN
cord-009318-zt1o1bcz	189	11	non	non	JJ
cord-009318-zt1o1bcz	189	12	-	-	JJ
cord-009318-zt1o1bcz	189	13	specific	specific	JJ
cord-009318-zt1o1bcz	189	14	amplification	amplification	NN
cord-009318-zt1o1bcz	189	15	could	could	MD
cord-009318-zt1o1bcz	189	16	arise	arise	VB
cord-009318-zt1o1bcz	189	17	from	from	IN
cord-009318-zt1o1bcz	189	18	mechanisms	mechanism	NNS
cord-009318-zt1o1bcz	189	19	similar	similar	JJ
cord-009318-zt1o1bcz	189	20	to	to	IN
cord-009318-zt1o1bcz	189	21	the	the	DT
cord-009318-zt1o1bcz	189	22	specific	specific	JJ
cord-009318-zt1o1bcz	189	23	products	product	NNS
cord-009318-zt1o1bcz	189	24	.	.	.
cord-009318-zt1o1bcz	190	1	Within	within	IN
cord-009318-zt1o1bcz	190	2	a	a	DT
cord-009318-zt1o1bcz	190	3	given	give	VBN
cord-009318-zt1o1bcz	190	4	bulk	bulk	JJ
cord-009318-zt1o1bcz	190	5	reaction	reaction	NN
cord-009318-zt1o1bcz	190	6	,	,	,
cord-009318-zt1o1bcz	190	7	variation	variation	NN
cord-009318-zt1o1bcz	190	8	in	in	IN
cord-009318-zt1o1bcz	190	9	randomer	randomer	NN
cord-009318-zt1o1bcz	190	10	sequence	sequence	NN
cord-009318-zt1o1bcz	190	11	length	length	NN
cord-009318-zt1o1bcz	190	12	and	and	CC
cord-009318-zt1o1bcz	190	13	identity	identity	NN
cord-009318-zt1o1bcz	190	14	was	be	VBD
cord-009318-zt1o1bcz	190	15	low	low	JJ
cord-009318-zt1o1bcz	190	16	.	.	.
cord-009318-zt1o1bcz	191	1	However	however	RB
cord-009318-zt1o1bcz	191	2	,	,	,
cord-009318-zt1o1bcz	191	3	between	between	IN
cord-009318-zt1o1bcz	191	4	different	different	JJ
cord-009318-zt1o1bcz	191	5	samples	sample	NNS
cord-009318-zt1o1bcz	191	6	,	,	,
cord-009318-zt1o1bcz	191	7	randomer	randomer	NN
cord-009318-zt1o1bcz	191	8	sequences	sequence	NNS
cord-009318-zt1o1bcz	191	9	of	of	IN
cord-009318-zt1o1bcz	191	10	multiple	multiple	JJ
cord-009318-zt1o1bcz	191	11	lengths	length	NNS
cord-009318-zt1o1bcz	191	12	and	and	CC
cord-009318-zt1o1bcz	191	13	identities	identity	NNS
cord-009318-zt1o1bcz	191	14	were	be	VBD
cord-009318-zt1o1bcz	191	15	observed	observe	VBN
cord-009318-zt1o1bcz	191	16	.	.	.
cord-009318-zt1o1bcz	192	1	These	these	DT
cord-009318-zt1o1bcz	192	2	two	two	CD
cord-009318-zt1o1bcz	192	3	results	result	NNS
cord-009318-zt1o1bcz	192	4	further	further	RB
cord-009318-zt1o1bcz	192	5	suggest	suggest	VBP
cord-009318-zt1o1bcz	192	6	that	that	IN
cord-009318-zt1o1bcz	192	7	in	in	IN
cord-009318-zt1o1bcz	192	8	bulk	bulk	JJ
cord-009318-zt1o1bcz	192	9	reactions	reaction	NNS
cord-009318-zt1o1bcz	192	10	amplification	amplification	NN
cord-009318-zt1o1bcz	192	11	occurs	occur	VBZ
cord-009318-zt1o1bcz	192	12	from	from	IN
cord-009318-zt1o1bcz	192	13	one	one	CD
cord-009318-zt1o1bcz	192	14	or	or	CC
cord-009318-zt1o1bcz	192	15	a	a	DT
cord-009318-zt1o1bcz	192	16	few	few	JJ
cord-009318-zt1o1bcz	192	17	sequences	sequence	NNS
cord-009318-zt1o1bcz	192	18	(	(	-LRB-
cord-009318-zt1o1bcz	192	19	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	192	20	Table	Table	NNP
cord-009318-zt1o1bcz	192	21	Groups	Groups	NNPS
cord-009318-zt1o1bcz	192	22	S5	S5	NNP
cord-009318-zt1o1bcz	192	23	-	-	HYPH
cord-009318-zt1o1bcz	192	24	7	7	CD
cord-009318-zt1o1bcz	192	25	)	)	-RRB-
cord-009318-zt1o1bcz	192	26	.	.	.
cord-009318-zt1o1bcz	193	1	Elongation	elongation	NN
cord-009318-zt1o1bcz	193	2	from	from	IN
cord-009318-zt1o1bcz	193	3	the	the	DT
cord-009318-zt1o1bcz	193	4	randomer	randomer	NN
cord-009318-zt1o1bcz	193	5	overhang	overhang	NN
cord-009318-zt1o1bcz	193	6	results	result	VBZ
cord-009318-zt1o1bcz	193	7	in	in	IN
cord-009318-zt1o1bcz	193	8	a	a	DT
cord-009318-zt1o1bcz	193	9	double	double	RB
cord-009318-zt1o1bcz	193	10	-	-	HYPH
cord-009318-zt1o1bcz	193	11	stranded	strand	VBN
cord-009318-zt1o1bcz	193	12	products	product	NNS
cord-009318-zt1o1bcz	193	13	,	,	,
cord-009318-zt1o1bcz	193	14	leading	lead	VBG
cord-009318-zt1o1bcz	193	15	to	to	IN
cord-009318-zt1o1bcz	193	16	dumbbell	dumbbell	JJ
cord-009318-zt1o1bcz	193	17	structures	structure	NNS
cord-009318-zt1o1bcz	193	18	,	,	,
cord-009318-zt1o1bcz	193	19	and	and	CC
cord-009318-zt1o1bcz	193	20	LAMP	lamp	NN
cord-009318-zt1o1bcz	193	21	-	-	HYPH
cord-009318-zt1o1bcz	193	22	like	like	JJ
cord-009318-zt1o1bcz	193	23	amplification	amplification	NN
cord-009318-zt1o1bcz	193	24	.	.	.
cord-009318-zt1o1bcz	194	1	First	first	RB
cord-009318-zt1o1bcz	194	2	,	,	,
cord-009318-zt1o1bcz	194	3	elongation	elongation	NN
cord-009318-zt1o1bcz	194	4	of	of	IN
cord-009318-zt1o1bcz	194	5	hairpins	hairpin	NNS
cord-009318-zt1o1bcz	194	6	with	with	IN
cord-009318-zt1o1bcz	194	7	complementary	complementary	JJ
cord-009318-zt1o1bcz	194	8	randomer	randomer	NN
cord-009318-zt1o1bcz	194	9	toe	toe	NN
cord-009318-zt1o1bcz	194	10	holds	hold	VBZ
cord-009318-zt1o1bcz	194	11	produces	produce	VBZ
cord-009318-zt1o1bcz	194	12	a	a	DT
cord-009318-zt1o1bcz	194	13	dimer	dimer	NN
cord-009318-zt1o1bcz	194	14	of	of	IN
cord-009318-zt1o1bcz	194	15	FIP	FIP	NNP
cord-009318-zt1o1bcz	194	16	-	-	HYPH
cord-009318-zt1o1bcz	194	17	BIP	BIP	NNP
cord-009318-zt1o1bcz	194	18	-	-	HYPH
cord-009318-zt1o1bcz	194	19	prcBIP	prcbip	NN
cord-009318-zt1o1bcz	194	20	-	-	HYPH
cord-009318-zt1o1bcz	194	21	rcFIP	rcfip	NN
cord-009318-zt1o1bcz	194	22	coupled	couple	VBN
cord-009318-zt1o1bcz	194	23	through	through	IN
cord-009318-zt1o1bcz	194	24	the	the	DT
cord-009318-zt1o1bcz	194	25	randomer	randomer	NN
cord-009318-zt1o1bcz	194	26	(	(	-LRB-
cord-009318-zt1o1bcz	194	27	(	(	-LRB-
cord-009318-zt1o1bcz	194	28	Figure	figure	NN
cord-009318-zt1o1bcz	194	29	4	4	CD
cord-009318-zt1o1bcz	194	30	-	-	SYM
cord-009318-zt1o1bcz	194	31	20	20	CD
cord-009318-zt1o1bcz	194	32	)	)	-RRB-
cord-009318-zt1o1bcz	194	33	.	.	.
cord-009318-zt1o1bcz	195	1	A	a	DT
cord-009318-zt1o1bcz	195	2	second	second	JJ
cord-009318-zt1o1bcz	195	3	priming	priming	NN
cord-009318-zt1o1bcz	195	4	of	of	IN
cord-009318-zt1o1bcz	195	5	the	the	DT
cord-009318-zt1o1bcz	195	6	hairpin	hairpin	NN
cord-009318-zt1o1bcz	195	7	by	by	IN
cord-009318-zt1o1bcz	195	8	BIP	BIP	NNP
cord-009318-zt1o1bcz	195	9	of	of	IN
cord-009318-zt1o1bcz	195	10	the	the	DT
cord-009318-zt1o1bcz	195	11	rcBIP	rcbip	NN
cord-009318-zt1o1bcz	195	12	-	-	HYPH
cord-009318-zt1o1bcz	195	13	pBIP	pbip	NN
cord-009318-zt1o1bcz	195	14	hairpin	hairpin	NN
cord-009318-zt1o1bcz	195	15	and	and	CC
cord-009318-zt1o1bcz	195	16	subsequent	subsequent	JJ
cord-009318-zt1o1bcz	195	17	elongation	elongation	NN
cord-009318-zt1o1bcz	195	18	,	,	,
cord-009318-zt1o1bcz	195	19	creates	create	VBZ
cord-009318-zt1o1bcz	195	20	a	a	DT
cord-009318-zt1o1bcz	195	21	new	new	JJ
cord-009318-zt1o1bcz	195	22	double	double	RB
cord-009318-zt1o1bcz	195	23	-	-	HYPH
cord-009318-zt1o1bcz	195	24	stranded	stranded	JJ
cord-009318-zt1o1bcz	195	25	product	product	NN
cord-009318-zt1o1bcz	195	26	and	and	CC
cord-009318-zt1o1bcz	195	27	reveals	reveal	VBZ
cord-009318-zt1o1bcz	195	28	a	a	DT
cord-009318-zt1o1bcz	195	29	self	self	NN
cord-009318-zt1o1bcz	195	30	-	-	HYPH
cord-009318-zt1o1bcz	195	31	priming	prime	VBG
cord-009318-zt1o1bcz	195	32	3	3	CD
cord-009318-zt1o1bcz	195	33	hairpin	hairpin	NN
cord-009318-zt1o1bcz	195	34	of	of	IN
cord-009318-zt1o1bcz	195	35	the	the	DT
cord-009318-zt1o1bcz	195	36	original	original	JJ
cord-009318-zt1o1bcz	195	37	strand	strand	NN
cord-009318-zt1o1bcz	195	38	(	(	-LRB-
cord-009318-zt1o1bcz	195	39	Figure	Figure	NNP
cord-009318-zt1o1bcz	195	40	4	4	CD
cord-009318-zt1o1bcz	195	41	-	-	SYM
cord-009318-zt1o1bcz	195	42	21	21	CD
cord-009318-zt1o1bcz	195	43	)	)	-RRB-
cord-009318-zt1o1bcz	195	44	.	.	.
cord-009318-zt1o1bcz	196	1	As	as	IN
cord-009318-zt1o1bcz	196	2	previously	previously	RB
cord-009318-zt1o1bcz	196	3	,	,	,
cord-009318-zt1o1bcz	196	4	upon	upon	IN
cord-009318-zt1o1bcz	196	5	elongation	elongation	NN
cord-009318-zt1o1bcz	196	6	,	,	,
cord-009318-zt1o1bcz	196	7	the	the	DT
cord-009318-zt1o1bcz	196	8	sequence	sequence	NN
cord-009318-zt1o1bcz	196	9	primed	prime	VBN
cord-009318-zt1o1bcz	196	10	by	by	IN
cord-009318-zt1o1bcz	196	11	BIP	BIP	NNP
cord-009318-zt1o1bcz	196	12	is	be	VBZ
cord-009318-zt1o1bcz	196	13	displaced	displace	VBN
cord-009318-zt1o1bcz	197	1	(	(	-LRB-
cord-009318-zt1o1bcz	197	2	Figure	figure	NN
cord-009318-zt1o1bcz	197	3	4	4	CD
cord-009318-zt1o1bcz	197	4	-	-	SYM
cord-009318-zt1o1bcz	197	5	22	22	CD
cord-009318-zt1o1bcz	197	6	)	)	-RRB-
cord-009318-zt1o1bcz	197	7	.	.	.
cord-009318-zt1o1bcz	198	1	Simultaneously	simultaneously	RB
cord-009318-zt1o1bcz	198	2	,	,	,
cord-009318-zt1o1bcz	198	3	the	the	DT
cord-009318-zt1o1bcz	198	4	self	self	NN
cord-009318-zt1o1bcz	198	5	-	-	HYPH
cord-009318-zt1o1bcz	198	6	priming	prime	VBG
cord-009318-zt1o1bcz	198	7	event	event	NN
cord-009318-zt1o1bcz	198	8	turns	turn	VBZ
cord-009318-zt1o1bcz	198	9	the	the	DT
cord-009318-zt1o1bcz	198	10	FIP	FIP	NNP
cord-009318-zt1o1bcz	198	11	-	-	HYPH
cord-009318-zt1o1bcz	198	12	BIP	BIP	NNP
cord-009318-zt1o1bcz	198	13	-	-	HYPH
cord-009318-zt1o1bcz	198	14	prcBIP	prcbip	NN
cord-009318-zt1o1bcz	198	15	trimer	trimer	NN
cord-009318-zt1o1bcz	198	16	to	to	IN
cord-009318-zt1o1bcz	198	17	a	a	DT
cord-009318-zt1o1bcz	198	18	pentamer	pentamer	NN
cord-009318-zt1o1bcz	198	19	,	,	,
cord-009318-zt1o1bcz	198	20	which	which	WDT
cord-009318-zt1o1bcz	198	21	may	may	MD
cord-009318-zt1o1bcz	198	22	continue	continue	VB
cord-009318-zt1o1bcz	198	23	to	to	TO
cord-009318-zt1o1bcz	198	24	be	be	VB
cord-009318-zt1o1bcz	198	25	amplified	amplify	VBN
cord-009318-zt1o1bcz	198	26	by	by	IN
cord-009318-zt1o1bcz	198	27	BIP	BIP	NNP
cord-009318-zt1o1bcz	198	28	.	.	.
cord-009318-zt1o1bcz	199	1	The	the	DT
cord-009318-zt1o1bcz	199	2	released	release	VBN
cord-009318-zt1o1bcz	199	3	sequence	sequence	NN
cord-009318-zt1o1bcz	199	4	(	(	-LRB-
cord-009318-zt1o1bcz	199	5	Figure	figure	NN
cord-009318-zt1o1bcz	199	6	4	4	CD
cord-009318-zt1o1bcz	199	7	-	-	SYM
cord-009318-zt1o1bcz	199	8	22	22	CD
cord-009318-zt1o1bcz	199	9	)	)	-RRB-
cord-009318-zt1o1bcz	199	10	is	be	VBZ
cord-009318-zt1o1bcz	199	11	again	again	RB
cord-009318-zt1o1bcz	199	12	self	self	NN
cord-009318-zt1o1bcz	199	13	-	-	HYPH
cord-009318-zt1o1bcz	199	14	priming	priming	NN
cord-009318-zt1o1bcz	199	15	,	,	,
cord-009318-zt1o1bcz	199	16	and	and	CC
cord-009318-zt1o1bcz	199	17	whose	whose	WP$
cord-009318-zt1o1bcz	199	18	product	product	NN
cord-009318-zt1o1bcz	199	19	is	be	VBZ
cord-009318-zt1o1bcz	199	20	equivalent	equivalent	JJ
cord-009318-zt1o1bcz	199	21	to	to	IN
cord-009318-zt1o1bcz	199	22	Figure	figure	NN
cord-009318-zt1o1bcz	199	23	4	4	CD
cord-009318-zt1o1bcz	199	24	-20	-20	CD
cord-009318-zt1o1bcz	199	25	to	to	TO
cord-009318-zt1o1bcz	199	26	restart	restart	VB
cord-009318-zt1o1bcz	199	27	the	the	DT
cord-009318-zt1o1bcz	199	28	cycle	cycle	NN
cord-009318-zt1o1bcz	199	29	.	.	.
cord-009318-zt1o1bcz	200	1	Further	further	RB
cord-009318-zt1o1bcz	200	2	,	,	,
cord-009318-zt1o1bcz	200	3	amplified	amplified	JJ
cord-009318-zt1o1bcz	200	4	hairpins	hairpin	NNS
cord-009318-zt1o1bcz	200	5	may	may	MD
cord-009318-zt1o1bcz	200	6	,	,	,
cord-009318-zt1o1bcz	200	7	in	in	IN
cord-009318-zt1o1bcz	200	8	addition	addition	NN
cord-009318-zt1o1bcz	200	9	to	to	IN
cord-009318-zt1o1bcz	200	10	BIP	BIP	NNP
cord-009318-zt1o1bcz	200	11	priming	prime	VBG
cord-009318-zt1o1bcz	200	12	of	of	IN
cord-009318-zt1o1bcz	200	13	the	the	DT
cord-009318-zt1o1bcz	200	14	hairpin	hairpin	NN
cord-009318-zt1o1bcz	200	15	,	,	,
cord-009318-zt1o1bcz	200	16	duplicate	duplicate	VB
cord-009318-zt1o1bcz	200	17	through	through	IN
cord-009318-zt1o1bcz	200	18	self	self	NN
cord-009318-zt1o1bcz	200	19	-	-	HYPH
cord-009318-zt1o1bcz	200	20	priming	priming	NN
cord-009318-zt1o1bcz	200	21	by	by	IN
cord-009318-zt1o1bcz	200	22	breathing	breathing	NN
cord-009318-zt1o1bcz	200	23	and	and	CC
cord-009318-zt1o1bcz	200	24	formation	formation	NN
cord-009318-zt1o1bcz	200	25	of	of	IN
cord-009318-zt1o1bcz	200	26	a	a	DT
cord-009318-zt1o1bcz	200	27	3	3	CD
cord-009318-zt1o1bcz	200	28	rcBIP	rcbip	NN
cord-009318-zt1o1bcz	200	29	-	-	HYPH
cord-009318-zt1o1bcz	200	30	pBIP	pbip	NN
cord-009318-zt1o1bcz	200	31	hairpin	hairpin	NN
cord-009318-zt1o1bcz	200	32	(	(	-LRB-
cord-009318-zt1o1bcz	200	33	Figure	Figure	NNP
cord-009318-zt1o1bcz	200	34	4	4	CD
cord-009318-zt1o1bcz	200	35	-	-	SYM
cord-009318-zt1o1bcz	200	36	23	23	CD
cord-009318-zt1o1bcz	200	37	)	)	-RRB-
cord-009318-zt1o1bcz	200	38	.	.	.
cord-009318-zt1o1bcz	201	1	The	the	DT
cord-009318-zt1o1bcz	201	2	products	product	NNS
cord-009318-zt1o1bcz	201	3	of	of	IN
cord-009318-zt1o1bcz	201	4	these	these	DT
cord-009318-zt1o1bcz	201	5	reactions	reaction	NNS
cord-009318-zt1o1bcz	201	6	are	be	VBP
cord-009318-zt1o1bcz	201	7	capable	capable	JJ
cord-009318-zt1o1bcz	201	8	of	of	IN
cord-009318-zt1o1bcz	201	9	forming	form	VBG
cord-009318-zt1o1bcz	201	10	a	a	DT
cord-009318-zt1o1bcz	201	11	branched	branched	JJ
cord-009318-zt1o1bcz	201	12	,	,	,
cord-009318-zt1o1bcz	201	13	mesh	mesh	NN
cord-009318-zt1o1bcz	201	14	-	-	HYPH
cord-009318-zt1o1bcz	201	15	like	like	JJ
cord-009318-zt1o1bcz	201	16	network	network	NN
cord-009318-zt1o1bcz	201	17	resulting	result	VBG
cord-009318-zt1o1bcz	201	18	in	in	IN
cord-009318-zt1o1bcz	201	19	the	the	DT
cord-009318-zt1o1bcz	201	20	observed	observed	JJ
cord-009318-zt1o1bcz	201	21	high	high	JJ
cord-009318-zt1o1bcz	201	22	temperature	temperature	NN
cord-009318-zt1o1bcz	201	23	melting	melting	NN
cord-009318-zt1o1bcz	201	24	.	.	.
cord-009318-zt1o1bcz	202	1	Products	product	NNS
cord-009318-zt1o1bcz	202	2	may	may	MD
cord-009318-zt1o1bcz	202	3	experience	experience	VB
cord-009318-zt1o1bcz	202	4	random	random	JJ
cord-009318-zt1o1bcz	202	5	internal	internal	JJ
cord-009318-zt1o1bcz	202	6	priming	priming	NN
cord-009318-zt1o1bcz	202	7	by	by	IN
cord-009318-zt1o1bcz	202	8	through	through	IN
cord-009318-zt1o1bcz	202	9	hairpin	hairpin	NN
cord-009318-zt1o1bcz	202	10	formation	formation	NN
cord-009318-zt1o1bcz	202	11	(	(	-LRB-
cord-009318-zt1o1bcz	202	12	e.g.	e.g.	RB
cord-009318-zt1o1bcz	202	13	.	.	.
cord-009318-zt1o1bcz	203	1	Furthermore	furthermore	RB
cord-009318-zt1o1bcz	203	2	,	,	,
cord-009318-zt1o1bcz	203	3	in	in	IN
cord-009318-zt1o1bcz	203	4	addition	addition	NN
cord-009318-zt1o1bcz	203	5	to	to	IN
cord-009318-zt1o1bcz	203	6	intramolecular	intramolecular	JJ
cord-009318-zt1o1bcz	203	7	bonding	bonding	NN
cord-009318-zt1o1bcz	203	8	,	,	,
cord-009318-zt1o1bcz	203	9	the	the	DT
cord-009318-zt1o1bcz	203	10	highly	highly	RB
cord-009318-zt1o1bcz	203	11	repetitive	repetitive	JJ
cord-009318-zt1o1bcz	203	12	nature	nature	NN
cord-009318-zt1o1bcz	203	13	of	of	IN
cord-009318-zt1o1bcz	203	14	these	these	DT
cord-009318-zt1o1bcz	203	15	products	product	NNS
cord-009318-zt1o1bcz	203	16	allows	allow	VBZ
cord-009318-zt1o1bcz	203	17	for	for	IN
cord-009318-zt1o1bcz	203	18	melting	melting	NN
cord-009318-zt1o1bcz	203	19	of	of	IN
cord-009318-zt1o1bcz	203	20	internal	internal	JJ
cord-009318-zt1o1bcz	203	21	fragments	fragment	NNS
cord-009318-zt1o1bcz	203	22	,	,	,
cord-009318-zt1o1bcz	203	23	which	which	WDT
cord-009318-zt1o1bcz	203	24	reanneal	reanneal	NN
cord-009318-zt1o1bcz	203	25	to	to	TO
cord-009318-zt1o1bcz	203	26	self	self	VB
cord-009318-zt1o1bcz	203	27	in	in	IN
cord-009318-zt1o1bcz	203	28	a	a	DT
cord-009318-zt1o1bcz	203	29	different	different	JJ
cord-009318-zt1o1bcz	203	30	conformation	conformation	NN
cord-009318-zt1o1bcz	203	31	,	,	,
cord-009318-zt1o1bcz	203	32	or	or	CC
cord-009318-zt1o1bcz	203	33	a	a	DT
cord-009318-zt1o1bcz	203	34	neighboring	neighboring	JJ
cord-009318-zt1o1bcz	203	35	strand	strand	NN
cord-009318-zt1o1bcz	203	36	.	.	.
cord-009318-zt1o1bcz	204	1	Though	though	IN
cord-009318-zt1o1bcz	204	2	the	the	DT
cord-009318-zt1o1bcz	204	3	initial	initial	JJ
cord-009318-zt1o1bcz	204	4	steps	step	NNS
cord-009318-zt1o1bcz	204	5	of	of	IN
cord-009318-zt1o1bcz	204	6	generating	generate	VBG
cord-009318-zt1o1bcz	204	7	a	a	DT
cord-009318-zt1o1bcz	204	8	double	double	RB
cord-009318-zt1o1bcz	204	9	-	-	HYPH
cord-009318-zt1o1bcz	204	10	stranded	strand	VBN
cord-009318-zt1o1bcz	204	11	hairpin	hairpin	NN
cord-009318-zt1o1bcz	204	12	will	will	MD
cord-009318-zt1o1bcz	204	13	be	be	VB
cord-009318-zt1o1bcz	204	14	unique	unique	JJ
cord-009318-zt1o1bcz	204	15	to	to	IN
cord-009318-zt1o1bcz	204	16	our	-PRON-	PRP$
cord-009318-zt1o1bcz	204	17	particular	particular	JJ
cord-009318-zt1o1bcz	204	18	primer	primer	NN
cord-009318-zt1o1bcz	204	19	set	set	NN
cord-009318-zt1o1bcz	204	20	,	,	,
cord-009318-zt1o1bcz	204	21	once	once	RB
cord-009318-zt1o1bcz	204	22	a	a	DT
cord-009318-zt1o1bcz	204	23	seed	seed	NN
cord-009318-zt1o1bcz	204	24	is	be	VBZ
cord-009318-zt1o1bcz	204	25	generated	generate	VBN
cord-009318-zt1o1bcz	204	26	,	,	,
cord-009318-zt1o1bcz	204	27	the	the	DT
cord-009318-zt1o1bcz	204	28	processes	process	NNS
cord-009318-zt1o1bcz	204	29	of	of	IN
cord-009318-zt1o1bcz	204	30	template	template	NN
cord-009318-zt1o1bcz	204	31	switching	switching	NN
cord-009318-zt1o1bcz	204	32	and	and	CC
cord-009318-zt1o1bcz	204	33	terminal	terminal	JJ
cord-009318-zt1o1bcz	204	34	transferase	transferase	NN
cord-009318-zt1o1bcz	204	35	activity	activity	NN
cord-009318-zt1o1bcz	204	36	should	should	MD
cord-009318-zt1o1bcz	204	37	be	be	VB
cord-009318-zt1o1bcz	204	38	a	a	DT
cord-009318-zt1o1bcz	204	39	general	general	JJ
cord-009318-zt1o1bcz	204	40	phenomenon	phenomenon	NN
cord-009318-zt1o1bcz	204	41	associated	associate	VBN
cord-009318-zt1o1bcz	204	42	with	with	IN
cord-009318-zt1o1bcz	204	43	non	non	JJ
cord-009318-zt1o1bcz	204	44	-	-	JJ
cord-009318-zt1o1bcz	204	45	specific	specific	JJ
cord-009318-zt1o1bcz	204	46	amplification	amplification	NN
cord-009318-zt1o1bcz	204	47	of	of	IN
cord-009318-zt1o1bcz	204	48	thermophilic	thermophilic	JJ
cord-009318-zt1o1bcz	204	49	polymerase	polymerase	NN
cord-009318-zt1o1bcz	204	50	resulting	result	VBG
cord-009318-zt1o1bcz	204	51	in	in	IN
cord-009318-zt1o1bcz	204	52	exponential	exponential	JJ
cord-009318-zt1o1bcz	204	53	amplification	amplification	NN
cord-009318-zt1o1bcz	204	54	.	.	.
cord-009318-zt1o1bcz	205	1	As	as	IN
cord-009318-zt1o1bcz	205	2	evidence	evidence	NN
cord-009318-zt1o1bcz	205	3	,	,	,
cord-009318-zt1o1bcz	205	4	when	when	WRB
cord-009318-zt1o1bcz	205	5	the	the	DT
cord-009318-zt1o1bcz	205	6	mechanism	mechanism	NN
cord-009318-zt1o1bcz	205	7	of	of	IN
cord-009318-zt1o1bcz	205	8	seed	seed	NN
cord-009318-zt1o1bcz	205	9	formation	formation	NN
cord-009318-zt1o1bcz	205	10	is	be	VBZ
cord-009318-zt1o1bcz	205	11	disrupted	disrupt	VBN
cord-009318-zt1o1bcz	205	12	through	through	IN
cord-009318-zt1o1bcz	205	13	elimination	elimination	NN
cord-009318-zt1o1bcz	205	14	of	of	IN
cord-009318-zt1o1bcz	205	15	the	the	DT
cord-009318-zt1o1bcz	205	16	microhomology	microhomology	NN
cord-009318-zt1o1bcz	205	17	,	,	,
cord-009318-zt1o1bcz	205	18	amplicons	amplicon	NNS
cord-009318-zt1o1bcz	205	19	with	with	IN
cord-009318-zt1o1bcz	205	20	high	high	JJ
cord-009318-zt1o1bcz	205	21	T	t	NN
cord-009318-zt1o1bcz	205	22	m	m	NN
cord-009318-zt1o1bcz	205	23	still	still	RB
cord-009318-zt1o1bcz	205	24	occur	occur	VBP
cord-009318-zt1o1bcz	205	25	,	,	,
cord-009318-zt1o1bcz	205	26	albeit	albeit	IN
cord-009318-zt1o1bcz	205	27	with	with	IN
cord-009318-zt1o1bcz	205	28	lower	low	JJR
cord-009318-zt1o1bcz	205	29	frequency	frequency	NN
cord-009318-zt1o1bcz	205	30	and	and	CC
cord-009318-zt1o1bcz	205	31	delayed	delay	VBN
cord-009318-zt1o1bcz	205	32	occurrence	occurrence	NN
cord-009318-zt1o1bcz	205	33	(	(	-LRB-
cord-009318-zt1o1bcz	205	34	Supplementary	supplementary	JJ
cord-009318-zt1o1bcz	205	35	Figures	figure	NNS
cord-009318-zt1o1bcz	205	36	S4	S4	NNP
cord-009318-zt1o1bcz	205	37	-	-	HYPH
cord-009318-zt1o1bcz	205	38	6	6	CD
cord-009318-zt1o1bcz	205	39	and	and	CC
cord-009318-zt1o1bcz	205	40	 	 	_SP
cord-009318-zt1o1bcz	205	41	Tables	table	NNS
cord-009318-zt1o1bcz	206	1	S3	S3	NNP
cord-009318-zt1o1bcz	206	2	-	-	HYPH
cord-009318-zt1o1bcz	206	3	4	4	CD
cord-009318-zt1o1bcz	206	4	)	)	-RRB-
cord-009318-zt1o1bcz	206	5	.	.	.
cord-009318-zt1o1bcz	207	1	Template	template	NN
cord-009318-zt1o1bcz	207	2	switching	switch	VBG
cord-009318-zt1o1bcz	207	3	and	and	CC
cord-009318-zt1o1bcz	207	4	non	non	JJ
cord-009318-zt1o1bcz	207	5	-	-	JJ
cord-009318-zt1o1bcz	207	6	template	template	JJ
cord-009318-zt1o1bcz	207	7	synthesis	synthesis	NN
cord-009318-zt1o1bcz	207	8	are	be	VBP
cord-009318-zt1o1bcz	207	9	100×	100×	CD
cord-009318-zt1o1bcz	207	10	slower	slow	JJR
cord-009318-zt1o1bcz	207	11	than	than	IN
cord-009318-zt1o1bcz	207	12	template	template	NN
cord-009318-zt1o1bcz	207	13	extension	extension	NN
cord-009318-zt1o1bcz	207	14	(	(	-LRB-
cord-009318-zt1o1bcz	207	15	33	33	CD
cord-009318-zt1o1bcz	207	16	)	)	-RRB-
cord-009318-zt1o1bcz	207	17	.	.	.
cord-009318-zt1o1bcz	208	1	However	however	RB
cord-009318-zt1o1bcz	208	2	,	,	,
cord-009318-zt1o1bcz	208	3	once	once	IN
cord-009318-zt1o1bcz	208	4	the	the	DT
cord-009318-zt1o1bcz	208	5	self	self	NN
cord-009318-zt1o1bcz	208	6	-	-	HYPH
cord-009318-zt1o1bcz	208	7	amplifying	amplify	VBG
cord-009318-zt1o1bcz	208	8	products	product	NNS
cord-009318-zt1o1bcz	208	9	are	be	VBP
cord-009318-zt1o1bcz	208	10	selected	select	VBN
cord-009318-zt1o1bcz	208	11	,	,	,
cord-009318-zt1o1bcz	208	12	the	the	DT
cord-009318-zt1o1bcz	208	13	reaction	reaction	NN
cord-009318-zt1o1bcz	208	14	follows	follow	VBZ
cord-009318-zt1o1bcz	208	15	standard	standard	JJ
cord-009318-zt1o1bcz	208	16	exponential	exponential	JJ
cord-009318-zt1o1bcz	208	17	LAMP	lamp	NN
cord-009318-zt1o1bcz	208	18	enrichment	enrichment	NN
cord-009318-zt1o1bcz	208	19	.	.	.
cord-009318-zt1o1bcz	209	1	Thus	thus	RB
cord-009318-zt1o1bcz	209	2	,	,	,
cord-009318-zt1o1bcz	209	3	accumulation	accumulation	NN
cord-009318-zt1o1bcz	209	4	of	of	IN
cord-009318-zt1o1bcz	209	5	a	a	DT
cord-009318-zt1o1bcz	209	6	sufficient	sufficient	JJ
cord-009318-zt1o1bcz	209	7	pool	pool	NN
cord-009318-zt1o1bcz	209	8	of	of	IN
cord-009318-zt1o1bcz	209	9	randomers	randomer	NNS
cord-009318-zt1o1bcz	209	10	may	may	MD
cord-009318-zt1o1bcz	209	11	take	take	VB
cord-009318-zt1o1bcz	209	12	time	time	NN
cord-009318-zt1o1bcz	209	13	,	,	,
cord-009318-zt1o1bcz	209	14	but	but	CC
cord-009318-zt1o1bcz	209	15	still	still	RB
cord-009318-zt1o1bcz	209	16	result	result	VB
cord-009318-zt1o1bcz	209	17	in	in	IN
cord-009318-zt1o1bcz	209	18	a	a	DT
cord-009318-zt1o1bcz	209	19	delayed	delay	VBN
cord-009318-zt1o1bcz	209	20	bulk	bulk	JJ
cord-009318-zt1o1bcz	209	21	exponential	exponential	JJ
cord-009318-zt1o1bcz	209	22	amplification	amplification	NN
cord-009318-zt1o1bcz	209	23	event	event	NN
cord-009318-zt1o1bcz	209	24	.	.	.
cord-009318-zt1o1bcz	210	1	Furthermore	furthermore	RB
cord-009318-zt1o1bcz	210	2	,	,	,
cord-009318-zt1o1bcz	210	3	should	should	MD
cord-009318-zt1o1bcz	210	4	a	a	DT
cord-009318-zt1o1bcz	210	5	hairpin	hairpin	NN
cord-009318-zt1o1bcz	210	6	with	with	IN
cord-009318-zt1o1bcz	210	7	attached	attach	VBN
cord-009318-zt1o1bcz	210	8	randomer	randomer	NN
cord-009318-zt1o1bcz	210	9	form	form	NN
cord-009318-zt1o1bcz	210	10	,	,	,
cord-009318-zt1o1bcz	210	11	it	-PRON-	PRP
cord-009318-zt1o1bcz	210	12	is	be	VBZ
cord-009318-zt1o1bcz	210	13	possible	possible	JJ
cord-009318-zt1o1bcz	210	14	that	that	IN
cord-009318-zt1o1bcz	210	15	the	the	DT
cord-009318-zt1o1bcz	210	16	rising	rise	VBG
cord-009318-zt1o1bcz	210	17	baseline	baseline	NN
cord-009318-zt1o1bcz	210	18	,	,	,
cord-009318-zt1o1bcz	210	19	attributed	attribute	VBN
cord-009318-zt1o1bcz	210	20	to	to	IN
cord-009318-zt1o1bcz	210	21	hairpin	hairpin	JJ
cord-009318-zt1o1bcz	210	22	formation	formation	NN
cord-009318-zt1o1bcz	210	23	(	(	-LRB-
cord-009318-zt1o1bcz	210	24	37	37	CD
cord-009318-zt1o1bcz	210	25	)	)	-RRB-
cord-009318-zt1o1bcz	210	26	,	,	,
cord-009318-zt1o1bcz	210	27	may	may	MD
cord-009318-zt1o1bcz	210	28	also	also	RB
cord-009318-zt1o1bcz	210	29	by	by	IN
cord-009318-zt1o1bcz	210	30	in	in	FW
cord-009318-zt1o1bcz	210	31	vitro	vitro	FW
cord-009318-zt1o1bcz	210	32	selection	selection	NN
cord-009318-zt1o1bcz	210	33	of	of	IN
cord-009318-zt1o1bcz	210	34	the	the	DT
cord-009318-zt1o1bcz	210	35	products	product	NNS
cord-009318-zt1o1bcz	210	36	,	,	,
cord-009318-zt1o1bcz	210	37	lead	lead	VB
cord-009318-zt1o1bcz	210	38	to	to	IN
cord-009318-zt1o1bcz	210	39	and	and	CC
cord-009318-zt1o1bcz	210	40	result	result	VB
cord-009318-zt1o1bcz	210	41	in	in	IN
cord-009318-zt1o1bcz	210	42	spontaneous	spontaneous	JJ
cord-009318-zt1o1bcz	210	43	exponential	exponential	JJ
cord-009318-zt1o1bcz	210	44	amplification	amplification	NN
cord-009318-zt1o1bcz	210	45	.	.	.
cord-009318-zt1o1bcz	211	1	To	to	TO
cord-009318-zt1o1bcz	211	2	study	study	VB
cord-009318-zt1o1bcz	211	3	specific	specific	JJ
cord-009318-zt1o1bcz	211	4	and	and	CC
cord-009318-zt1o1bcz	211	5	non	non	JJ
cord-009318-zt1o1bcz	211	6	-	-	JJ
cord-009318-zt1o1bcz	211	7	specific	specific	JJ
cord-009318-zt1o1bcz	211	8	amplification	amplification	NN
cord-009318-zt1o1bcz	211	9	events	event	NNS
cord-009318-zt1o1bcz	211	10	at	at	IN
cord-009318-zt1o1bcz	211	11	the	the	DT
cord-009318-zt1o1bcz	211	12	digital	digital	JJ
cord-009318-zt1o1bcz	211	13	single	single	JJ
cord-009318-zt1o1bcz	211	14	-	-	HYPH
cord-009318-zt1o1bcz	211	15	molecule	molecule	NN
cord-009318-zt1o1bcz	211	16	level	level	NN
cord-009318-zt1o1bcz	211	17	,	,	,
cord-009318-zt1o1bcz	211	18	we	-PRON-	PRP
cord-009318-zt1o1bcz	211	19	developed	develop	VBD
cord-009318-zt1o1bcz	211	20	a	a	DT
cord-009318-zt1o1bcz	211	21	new	new	JJ
cord-009318-zt1o1bcz	211	22	approach	approach	NN
cord-009318-zt1o1bcz	211	23	that	that	WDT
cord-009318-zt1o1bcz	211	24	enabled	enable	VBD
cord-009318-zt1o1bcz	211	25	HRM	HRM	NNP
cord-009318-zt1o1bcz	211	26	analysis	analysis	NN
cord-009318-zt1o1bcz	211	27	(	(	-LRB-
cord-009318-zt1o1bcz	211	28	obtaining	obtain	VBG
cord-009318-zt1o1bcz	211	29	'	'	``
cord-009318-zt1o1bcz	211	30	melt	melt	NN
cord-009318-zt1o1bcz	211	31	curves	curve	NNS
cord-009318-zt1o1bcz	211	32	'	'	''
cord-009318-zt1o1bcz	211	33	)	)	-RRB-
cord-009318-zt1o1bcz	211	34	to	to	TO
cord-009318-zt1o1bcz	211	35	be	be	VB
cord-009318-zt1o1bcz	211	36	performed	perform	VBN
cord-009318-zt1o1bcz	211	37	on	on	IN
cord-009318-zt1o1bcz	211	38	each	each	DT
cord-009318-zt1o1bcz	211	39	partition	partition	NN
cord-009318-zt1o1bcz	211	40	.	.	.
cord-009318-zt1o1bcz	212	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	212	2	used	use	VBD
cord-009318-zt1o1bcz	212	3	a	a	DT
cord-009318-zt1o1bcz	212	4	commercially	commercially	RB
cord-009318-zt1o1bcz	212	5	available	available	JJ
cord-009318-zt1o1bcz	212	6	microfluidic	microfluidic	JJ
cord-009318-zt1o1bcz	212	7	chip	chip	NN
cord-009318-zt1o1bcz	212	8	with	with	IN
cord-009318-zt1o1bcz	212	9	20,000	20,000	CD
cord-009318-zt1o1bcz	212	10	partitions	partition	NNS
cord-009318-zt1o1bcz	212	11	and	and	CC
cord-009318-zt1o1bcz	212	12	a	a	DT
cord-009318-zt1o1bcz	212	13	previously	previously	RB
cord-009318-zt1o1bcz	212	14	published	publish	VBN
cord-009318-zt1o1bcz	212	15	open	open	JJ
cord-009318-zt1o1bcz	212	16	-	-	HYPH
cord-009318-zt1o1bcz	212	17	source	source	NN
cord-009318-zt1o1bcz	212	18	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	212	19	method	method	NN
cord-009318-zt1o1bcz	212	20	accessible	accessible	JJ
cord-009318-zt1o1bcz	212	21	to	to	IN
cord-009318-zt1o1bcz	212	22	most	most	JJS
cord-009318-zt1o1bcz	212	23	standard	standard	JJ
cord-009318-zt1o1bcz	212	24	laboratories	laboratory	NNS
cord-009318-zt1o1bcz	212	25	(	(	-LRB-
cord-009318-zt1o1bcz	212	26	23	23	CD
cord-009318-zt1o1bcz	212	27	)	)	-RRB-
cord-009318-zt1o1bcz	212	28	with	with	IN
cord-009318-zt1o1bcz	212	29	the	the	DT
cord-009318-zt1o1bcz	212	30	fol	fol	NN
cord-009318-zt1o1bcz	212	31	-	-	HYPH
cord-009318-zt1o1bcz	212	32	lowing	low	VBG
cord-009318-zt1o1bcz	212	33	improvements	improvement	NNS
cord-009318-zt1o1bcz	212	34	:	:	:
cord-009318-zt1o1bcz	212	35	incorporation	incorporation	NN
cord-009318-zt1o1bcz	212	36	of	of	IN
cord-009318-zt1o1bcz	212	37	an	an	DT
cord-009318-zt1o1bcz	212	38	off	off	IN
cord-009318-zt1o1bcz	212	39	-	-	HYPH
cord-009318-zt1o1bcz	212	40	the	the	DT
cord-009318-zt1o1bcz	212	41	-	-	HYPH
cord-009318-zt1o1bcz	212	42	shelf	shelf	NN
cord-009318-zt1o1bcz	212	43	thermoelectric	thermoelectric	JJ
cord-009318-zt1o1bcz	212	44	unit	unit	NN
cord-009318-zt1o1bcz	212	45	to	to	IN
cord-009318-zt1o1bcz	212	46	both	both	CC
cord-009318-zt1o1bcz	212	47	heat	heat	VB
cord-009318-zt1o1bcz	212	48	and	and	CC
cord-009318-zt1o1bcz	212	49	cool	cool	VB
cord-009318-zt1o1bcz	212	50	the	the	DT
cord-009318-zt1o1bcz	212	51	chips	chip	NNS
cord-009318-zt1o1bcz	212	52	,	,	,
cord-009318-zt1o1bcz	212	53	and	and	CC
cord-009318-zt1o1bcz	212	54	an	an	DT
cord-009318-zt1o1bcz	212	55	enhanced	enhance	VBN
cord-009318-zt1o1bcz	212	56	MATLAB	MATLAB	NNP
cord-009318-zt1o1bcz	212	57	script	script	NN
cord-009318-zt1o1bcz	212	58	to	to	TO
cord-009318-zt1o1bcz	212	59	allow	allow	VB
cord-009318-zt1o1bcz	212	60	for	for	IN
cord-009318-zt1o1bcz	212	61	multicolor	multicolor	JJ
cord-009318-zt1o1bcz	212	62	tracking	tracking	NN
cord-009318-zt1o1bcz	212	63	.	.	.
cord-009318-zt1o1bcz	213	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	213	2	used	use	VBD
cord-009318-zt1o1bcz	213	3	the	the	DT
cord-009318-zt1o1bcz	213	4	temperature	temperature	NN
cord-009318-zt1o1bcz	213	5	-	-	HYPH
cord-009318-zt1o1bcz	213	6	independent	independent	JJ
cord-009318-zt1o1bcz	213	7	fluorophore	fluorophore	NN
cord-009318-zt1o1bcz	213	8	ROX	ROX	NNP
cord-009318-zt1o1bcz	213	9	to	to	TO
cord-009318-zt1o1bcz	213	10	track	track	VB
cord-009318-zt1o1bcz	213	11	each	each	DT
cord-009318-zt1o1bcz	213	12	partition	partition	NN
cord-009318-zt1o1bcz	213	13	's	's	POS
cord-009318-zt1o1bcz	213	14	location	location	NN
cord-009318-zt1o1bcz	213	15	and	and	CC
cord-009318-zt1o1bcz	213	16	the	the	DT
cord-009318-zt1o1bcz	213	17	dsDNA	dsdna	JJ
cord-009318-zt1o1bcz	213	18	intercalating	intercalating	NN
cord-009318-zt1o1bcz	213	19	fluorophore	fluorophore	NN
cord-009318-zt1o1bcz	213	20	SYTO	SYTO	NNP
cord-009318-zt1o1bcz	213	21	9	9	CD
cord-009318-zt1o1bcz	213	22	to	to	TO
cord-009318-zt1o1bcz	213	23	follow	follow	VB
cord-009318-zt1o1bcz	213	24	amplification	amplification	NN
cord-009318-zt1o1bcz	213	25	and	and	CC
cord-009318-zt1o1bcz	213	26	hybridization	hybridization	NN
cord-009318-zt1o1bcz	213	27	status	status	NN
cord-009318-zt1o1bcz	213	28	.	.	.
cord-009318-zt1o1bcz	214	1	This	this	DT
cord-009318-zt1o1bcz	214	2	two	two	CD
cord-009318-zt1o1bcz	214	3	-	-	HYPH
cord-009318-zt1o1bcz	214	4	channel	channel	NN
cord-009318-zt1o1bcz	214	5	approach	approach	NN
cord-009318-zt1o1bcz	214	6	is	be	VBZ
cord-009318-zt1o1bcz	214	7	required	require	VBN
cord-009318-zt1o1bcz	214	8	to	to	TO
cord-009318-zt1o1bcz	214	9	follow	follow	VB
cord-009318-zt1o1bcz	214	10	a	a	DT
cord-009318-zt1o1bcz	214	11	partition	partition	NN
cord-009318-zt1o1bcz	214	12	through	through	IN
cord-009318-zt1o1bcz	214	13	both	both	CC
cord-009318-zt1o1bcz	214	14	amplification	amplification	NN
cord-009318-zt1o1bcz	214	15	and	and	CC
cord-009318-zt1o1bcz	214	16	the	the	DT
cord-009318-zt1o1bcz	214	17	entirety	entirety	NN
cord-009318-zt1o1bcz	214	18	of	of	IN
cord-009318-zt1o1bcz	214	19	the	the	DT
cord-009318-zt1o1bcz	214	20	HRM	HRM	NNP
cord-009318-zt1o1bcz	214	21	when	when	WRB
cord-009318-zt1o1bcz	214	22	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	214	23	from	from	IN
cord-009318-zt1o1bcz	214	24	SYTO	SYTO	NNP
cord-009318-zt1o1bcz	214	25	9	9	CD
cord-009318-zt1o1bcz	214	26	is	be	VBZ
cord-009318-zt1o1bcz	214	27	lost	lose	VBN
cord-009318-zt1o1bcz	214	28	.	.	.
cord-009318-zt1o1bcz	215	1	As	as	IN
cord-009318-zt1o1bcz	215	2	an	an	DT
cord-009318-zt1o1bcz	215	3	illustration	illustration	NN
cord-009318-zt1o1bcz	215	4	of	of	IN
cord-009318-zt1o1bcz	215	5	the	the	DT
cord-009318-zt1o1bcz	215	6	capabilities	capability	NNS
cord-009318-zt1o1bcz	215	7	of	of	IN
cord-009318-zt1o1bcz	215	8	our	-PRON-	PRP$
cord-009318-zt1o1bcz	215	9	approach	approach	NN
cord-009318-zt1o1bcz	215	10	,	,	,
cord-009318-zt1o1bcz	215	11	we	-PRON-	PRP
cord-009318-zt1o1bcz	215	12	first	first	RB
cord-009318-zt1o1bcz	215	13	used	use	VBD
cord-009318-zt1o1bcz	215	14	real	real	JJ
cord-009318-zt1o1bcz	215	15	-	-	HYPH
cord-009318-zt1o1bcz	215	16	time	time	NN
cord-009318-zt1o1bcz	215	17	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	215	18	to	to	TO
cord-009318-zt1o1bcz	215	19	study	study	VB
cord-009318-zt1o1bcz	215	20	the	the	DT
cord-009318-zt1o1bcz	215	21	kinetic	kinetic	JJ
cord-009318-zt1o1bcz	215	22	parameters	parameter	NNS
cord-009318-zt1o1bcz	215	23	of	of	IN
cord-009318-zt1o1bcz	215	24	individual	individual	JJ
cord-009318-zt1o1bcz	215	25	reactions	reaction	NNS
cord-009318-zt1o1bcz	215	26	and	and	CC
cord-009318-zt1o1bcz	215	27	we	-PRON-	PRP
cord-009318-zt1o1bcz	215	28	used	use	VBD
cord-009318-zt1o1bcz	215	29	T	T	NNP
cord-009318-zt1o1bcz	215	30	m	m	NNP
cord-009318-zt1o1bcz	215	31	to	to	TO
cord-009318-zt1o1bcz	215	32	classify	classify	VB
cord-009318-zt1o1bcz	215	33	reaction	reaction	NN
cord-009318-zt1o1bcz	215	34	outcome	outcome	NN
cord-009318-zt1o1bcz	215	35	(	(	-LRB-
cord-009318-zt1o1bcz	215	36	Figure	figure	NN
cord-009318-zt1o1bcz	215	37	5	5	CD
cord-009318-zt1o1bcz	215	38	)	)	-RRB-
cord-009318-zt1o1bcz	215	39	.	.	.
cord-009318-zt1o1bcz	216	1	Using	use	VBG
cord-009318-zt1o1bcz	216	2	real	real	JJ
cord-009318-zt1o1bcz	216	3	-	-	HYPH
cord-009318-zt1o1bcz	216	4	time	time	NN
cord-009318-zt1o1bcz	216	5	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	216	6	,	,	,
cord-009318-zt1o1bcz	216	7	we	-PRON-	PRP
cord-009318-zt1o1bcz	216	8	followed	follow	VBD
cord-009318-zt1o1bcz	216	9	individual	individual	JJ
cord-009318-zt1o1bcz	216	10	partitions	partition	NNS
cord-009318-zt1o1bcz	216	11	as	as	IN
cord-009318-zt1o1bcz	216	12	they	-PRON-	PRP
cord-009318-zt1o1bcz	216	13	amplified	amplify	VBD
cord-009318-zt1o1bcz	216	14	as	as	IN
cord-009318-zt1o1bcz	216	15	a	a	DT
cord-009318-zt1o1bcz	216	16	function	function	NN
cord-009318-zt1o1bcz	216	17	of	of	IN
cord-009318-zt1o1bcz	216	18	time	time	NN
cord-009318-zt1o1bcz	216	19	(	(	-LRB-
cord-009318-zt1o1bcz	216	20	Figure	figure	NN
cord-009318-zt1o1bcz	216	21	5A	5a	NN
cord-009318-zt1o1bcz	216	22	)	)	-RRB-
cord-009318-zt1o1bcz	216	23	and	and	CC
cord-009318-zt1o1bcz	216	24	then	then	RB
cord-009318-zt1o1bcz	216	25	by	by	IN
cord-009318-zt1o1bcz	216	26	temperature	temperature	NN
cord-009318-zt1o1bcz	216	27	as	as	IN
cord-009318-zt1o1bcz	216	28	they	-PRON-	PRP
cord-009318-zt1o1bcz	216	29	went	go	VBD
cord-009318-zt1o1bcz	216	30	through	through	IN
cord-009318-zt1o1bcz	216	31	HRM	HRM	NNP
cord-009318-zt1o1bcz	217	1	(	(	-LRB-
cord-009318-zt1o1bcz	217	2	Figure	figure	NN
cord-009318-zt1o1bcz	217	3	5B	5b	NN
cord-009318-zt1o1bcz	217	4	)	)	-RRB-
cord-009318-zt1o1bcz	217	5	.	.	.
cord-009318-zt1o1bcz	218	1	Real	real	JJ
cord-009318-zt1o1bcz	218	2	-	-	HYPH
cord-009318-zt1o1bcz	218	3	time	time	NN
cord-009318-zt1o1bcz	218	4	imaging	imaging	NN
cord-009318-zt1o1bcz	218	5	of	of	IN
cord-009318-zt1o1bcz	218	6	individual	individual	JJ
cord-009318-zt1o1bcz	218	7	partitions	partition	NNS
cord-009318-zt1o1bcz	218	8	enables	enable	VBZ
cord-009318-zt1o1bcz	218	9	us	-PRON-	PRP
cord-009318-zt1o1bcz	218	10	to	to	TO
cord-009318-zt1o1bcz	218	11	reconstruct	reconstruct	VB
cord-009318-zt1o1bcz	218	12	the	the	DT
cord-009318-zt1o1bcz	218	13	standard	standard	JJ
cord-009318-zt1o1bcz	218	14	amplification	amplification	NN
cord-009318-zt1o1bcz	218	15	curves	curve	NNS
cord-009318-zt1o1bcz	218	16	of	of	IN
cord-009318-zt1o1bcz	218	17	intensity	intensity	NN
cord-009318-zt1o1bcz	218	18	for	for	IN
cord-009318-zt1o1bcz	218	19	each	each	DT
cord-009318-zt1o1bcz	218	20	partition	partition	NN
cord-009318-zt1o1bcz	218	21	as	as	IN
cord-009318-zt1o1bcz	218	22	a	a	DT
cord-009318-zt1o1bcz	218	23	function	function	NN
cord-009318-zt1o1bcz	218	24	of	of	IN
cord-009318-zt1o1bcz	218	25	time	time	NN
cord-009318-zt1o1bcz	218	26	(	(	-LRB-
cord-009318-zt1o1bcz	218	27	Figure	figure	NN
cord-009318-zt1o1bcz	218	28	5C	5c	CD
cord-009318-zt1o1bcz	218	29	)	)	-RRB-
cord-009318-zt1o1bcz	218	30	,	,	,
cord-009318-zt1o1bcz	218	31	and	and	CC
cord-009318-zt1o1bcz	218	32	plotting	plot	VBG
cord-009318-zt1o1bcz	218	33	the	the	DT
cord-009318-zt1o1bcz	218	34	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	218	35	intensity	intensity	NN
cord-009318-zt1o1bcz	218	36	as	as	IN
cord-009318-zt1o1bcz	218	37	a	a	DT
cord-009318-zt1o1bcz	218	38	function	function	NN
cord-009318-zt1o1bcz	218	39	of	of	IN
cord-009318-zt1o1bcz	218	40	temperature	temperature	NN
cord-009318-zt1o1bcz	218	41	yields	yield	VBZ
cord-009318-zt1o1bcz	218	42	an	an	DT
cord-009318-zt1o1bcz	218	43	HRM	HRM	NNP
cord-009318-zt1o1bcz	218	44	trace	trace	NN
cord-009318-zt1o1bcz	218	45	(	(	-LRB-
cord-009318-zt1o1bcz	218	46	Figure	figure	NN
cord-009318-zt1o1bcz	218	47	5D	5d	CD
cord-009318-zt1o1bcz	218	48	)	)	-RRB-
cord-009318-zt1o1bcz	218	49	;	;	:
cord-009318-zt1o1bcz	218	50	the	the	DT
cord-009318-zt1o1bcz	218	51	negative	negative	JJ
cord-009318-zt1o1bcz	218	52	derivative	derivative	JJ
cord-009318-zt1o1bcz	218	53	plot	plot	NN
cord-009318-zt1o1bcz	218	54	(	(	-LRB-
cord-009318-zt1o1bcz	218	55	Figure	figure	NN
cord-009318-zt1o1bcz	218	56	5E	5e	NN
cord-009318-zt1o1bcz	218	57	)	)	-RRB-
cord-009318-zt1o1bcz	218	58	of	of	IN
cord-009318-zt1o1bcz	218	59	this	this	DT
cord-009318-zt1o1bcz	218	60	melt	melt	NN
cord-009318-zt1o1bcz	218	61	trace	trace	NN
cord-009318-zt1o1bcz	218	62	is	be	VBZ
cord-009318-zt1o1bcz	218	63	the	the	DT
cord-009318-zt1o1bcz	218	64	standard	standard	JJ
cord-009318-zt1o1bcz	218	65	melt	melt	NN
cord-009318-zt1o1bcz	218	66	curve	curve	NN
cord-009318-zt1o1bcz	218	67	.	.	.
cord-009318-zt1o1bcz	219	1	Analogous	analogous	JJ
cord-009318-zt1o1bcz	219	2	to	to	IN
cord-009318-zt1o1bcz	219	3	bulk	bulk	JJ
cord-009318-zt1o1bcz	219	4	measurements	measurement	NNS
cord-009318-zt1o1bcz	219	5	,	,	,
cord-009318-zt1o1bcz	219	6	the	the	DT
cord-009318-zt1o1bcz	219	7	standard	standard	JJ
cord-009318-zt1o1bcz	219	8	melt	melt	NN
cord-009318-zt1o1bcz	219	9	curve	curve	NN
cord-009318-zt1o1bcz	219	10	is	be	VBZ
cord-009318-zt1o1bcz	219	11	used	use	VBN
cord-009318-zt1o1bcz	219	12	to	to	TO
cord-009318-zt1o1bcz	219	13	classify	classify	VB
cord-009318-zt1o1bcz	219	14	reactions	reaction	NNS
cord-009318-zt1o1bcz	219	15	as	as	IN
cord-009318-zt1o1bcz	219	16	specific	specific	JJ
cord-009318-zt1o1bcz	219	17	or	or	CC
cord-009318-zt1o1bcz	219	18	non	non	JJ
cord-009318-zt1o1bcz	219	19	-	-	JJ
cord-009318-zt1o1bcz	219	20	specific	specific	JJ
cord-009318-zt1o1bcz	219	21	.	.	.
cord-009318-zt1o1bcz	220	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	220	2	used	use	VBD
cord-009318-zt1o1bcz	220	3	these	these	DT
cord-009318-zt1o1bcz	220	4	classifications	classification	NNS
cord-009318-zt1o1bcz	220	5	to	to	TO
cord-009318-zt1o1bcz	220	6	identify	identify	VB
cord-009318-zt1o1bcz	220	7	important	important	JJ
cord-009318-zt1o1bcz	220	8	patterns	pattern	NNS
cord-009318-zt1o1bcz	220	9	in	in	IN
cord-009318-zt1o1bcz	220	10	the	the	DT
cord-009318-zt1o1bcz	220	11	kinetics	kinetic	NNS
cord-009318-zt1o1bcz	220	12	of	of	IN
cord-009318-zt1o1bcz	220	13	each	each	DT
cord-009318-zt1o1bcz	220	14	type	type	NN
cord-009318-zt1o1bcz	220	15	of	of	IN
cord-009318-zt1o1bcz	220	16	amplification	amplification	NN
cord-009318-zt1o1bcz	220	17	(	(	-LRB-
cord-009318-zt1o1bcz	220	18	Figure	figure	NN
cord-009318-zt1o1bcz	220	19	5F	5f	NN
cord-009318-zt1o1bcz	220	20	-H	-H	NNS
cord-009318-zt1o1bcz	220	21	)	)	-RRB-
cord-009318-zt1o1bcz	220	22	.	.	.
cord-009318-zt1o1bcz	221	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	221	2	next	next	RB
cord-009318-zt1o1bcz	221	3	used	use	VBD
cord-009318-zt1o1bcz	221	4	real	real	JJ
cord-009318-zt1o1bcz	221	5	-	-	HYPH
cord-009318-zt1o1bcz	221	6	time	time	NN
cord-009318-zt1o1bcz	221	7	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	221	8	with	with	IN
cord-009318-zt1o1bcz	221	9	HRM	HRM	NNP
cord-009318-zt1o1bcz	221	10	to	to	TO
cord-009318-zt1o1bcz	221	11	determine	determine	VB
cord-009318-zt1o1bcz	221	12	whether	whether	IN
cord-009318-zt1o1bcz	221	13	differences	difference	NNS
cord-009318-zt1o1bcz	221	14	in	in	IN
cord-009318-zt1o1bcz	221	15	time	time	NN
cord-009318-zt1o1bcz	221	16	to	to	IN
cord-009318-zt1o1bcz	221	17	positive	positive	JJ
cord-009318-zt1o1bcz	221	18	(	(	-LRB-
cord-009318-zt1o1bcz	221	19	TTP	TTP	NNP
cord-009318-zt1o1bcz	221	20	)	)	-RRB-
cord-009318-zt1o1bcz	221	21	were	be	VBD
cord-009318-zt1o1bcz	221	22	due	due	JJ
cord-009318-zt1o1bcz	221	23	to	to	IN
cord-009318-zt1o1bcz	221	24	a	a	DT
cord-009318-zt1o1bcz	221	25	difference	difference	NN
cord-009318-zt1o1bcz	221	26	in	in	IN
cord-009318-zt1o1bcz	221	27	amplification	amplification	NN
cord-009318-zt1o1bcz	221	28	initiation	initiation	NN
cord-009318-zt1o1bcz	221	29	or	or	CC
cord-009318-zt1o1bcz	221	30	in	in	IN
cord-009318-zt1o1bcz	221	31	rate	rate	NN
cord-009318-zt1o1bcz	221	32	.	.	.
cord-009318-zt1o1bcz	222	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	222	2	expect	expect	VBP
cord-009318-zt1o1bcz	222	3	this	this	DT
cord-009318-zt1o1bcz	222	4	information	information	NN
cord-009318-zt1o1bcz	222	5	would	would	MD
cord-009318-zt1o1bcz	222	6	be	be	VB
cord-009318-zt1o1bcz	222	7	valuable	valuable	JJ
cord-009318-zt1o1bcz	222	8	for	for	IN
cord-009318-zt1o1bcz	222	9	elucidating	elucidate	VBG
cord-009318-zt1o1bcz	222	10	whether	whether	IN
cord-009318-zt1o1bcz	222	11	the	the	DT
cord-009318-zt1o1bcz	222	12	molecules	molecule	NNS
cord-009318-zt1o1bcz	222	13	that	that	WDT
cord-009318-zt1o1bcz	222	14	lead	lead	VBP
cord-009318-zt1o1bcz	222	15	to	to	IN
cord-009318-zt1o1bcz	222	16	bulk	bulk	JJ
cord-009318-zt1o1bcz	222	17	amplification	amplification	NN
cord-009318-zt1o1bcz	222	18	are	be	VBP
cord-009318-zt1o1bcz	222	19	the	the	DT
cord-009318-zt1o1bcz	222	20	ones	one	NNS
cord-009318-zt1o1bcz	222	21	that	that	WDT
cord-009318-zt1o1bcz	222	22	are	be	VBP
cord-009318-zt1o1bcz	222	23	first	first	JJ
cord-009318-zt1o1bcz	222	24	to	to	TO
cord-009318-zt1o1bcz	222	25	initiate	initiate	VB
cord-009318-zt1o1bcz	222	26	or	or	CC
cord-009318-zt1o1bcz	222	27	the	the	DT
cord-009318-zt1o1bcz	222	28	ones	one	NNS
cord-009318-zt1o1bcz	222	29	that	that	WDT
cord-009318-zt1o1bcz	222	30	initiate	initiate	VBP
cord-009318-zt1o1bcz	222	31	with	with	IN
cord-009318-zt1o1bcz	222	32	the	the	DT
cord-009318-zt1o1bcz	222	33	fastest	fast	JJS
cord-009318-zt1o1bcz	222	34	rates	rate	NNS
cord-009318-zt1o1bcz	222	35	.	.	.
cord-009318-zt1o1bcz	223	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	223	2	found	find	VBD
cord-009318-zt1o1bcz	223	3	that	that	IN
cord-009318-zt1o1bcz	223	4	TTP	TTP	NNP
cord-009318-zt1o1bcz	223	5	can	can	MD
cord-009318-zt1o1bcz	223	6	be	be	VB
cord-009318-zt1o1bcz	223	7	heterogeneous	heterogeneous	JJ
cord-009318-zt1o1bcz	223	8	while	while	IN
cord-009318-zt1o1bcz	223	9	T	T	NNP
cord-009318-zt1o1bcz	223	10	m	m	NNP
cord-009318-zt1o1bcz	223	11	is	be	VBZ
cord-009318-zt1o1bcz	223	12	constant	constant	JJ
cord-009318-zt1o1bcz	223	13	(	(	-LRB-
cord-009318-zt1o1bcz	223	14	28.6	28.6	CD
cord-009318-zt1o1bcz	223	15	±	±	CD
cord-009318-zt1o1bcz	223	16	8.9	8.9	CD
cord-009318-zt1o1bcz	223	17	min	min	NN
cord-009318-zt1o1bcz	223	18	with	with	IN
cord-009318-zt1o1bcz	224	1	87.5	87.5	NNP
cord-009318-zt1o1bcz	224	2	±	±	NNP
cord-009318-zt1o1bcz	224	3	0.2	0.2	CD
cord-009318-zt1o1bcz	224	4	•	•	NN
cord-009318-zt1o1bcz	224	5	C	C	NNP
cord-009318-zt1o1bcz	224	6	)	)	-RRB-
cord-009318-zt1o1bcz	224	7	,	,	.
cord-009318-zt1o1bcz	225	1	indicating	indicate	VBG
cord-009318-zt1o1bcz	225	2	that	that	IN
cord-009318-zt1o1bcz	225	3	the	the	DT
cord-009318-zt1o1bcz	225	4	same	same	JJ
cord-009318-zt1o1bcz	225	5	product	product	NN
cord-009318-zt1o1bcz	225	6	may	may	MD
cord-009318-zt1o1bcz	225	7	initiate	initiate	VB
cord-009318-zt1o1bcz	225	8	at	at	IN
cord-009318-zt1o1bcz	225	9	different	different	JJ
cord-009318-zt1o1bcz	225	10	times	time	NNS
cord-009318-zt1o1bcz	225	11	(	(	-LRB-
cord-009318-zt1o1bcz	225	12	Figure	figure	NN
cord-009318-zt1o1bcz	225	13	5F	5f	NN
cord-009318-zt1o1bcz	225	14	)	)	-RRB-
cord-009318-zt1o1bcz	225	15	.	.	.
cord-009318-zt1o1bcz	226	1	This	this	DT
cord-009318-zt1o1bcz	226	2	is	be	VBZ
cord-009318-zt1o1bcz	226	3	consistent	consistent	JJ
cord-009318-zt1o1bcz	226	4	with	with	IN
cord-009318-zt1o1bcz	226	5	our	-PRON-	PRP$
cord-009318-zt1o1bcz	226	6	knowledge	knowledge	NN
cord-009318-zt1o1bcz	226	7	of	of	IN
cord-009318-zt1o1bcz	226	8	the	the	DT
cord-009318-zt1o1bcz	226	9	stochastic	stochastic	JJ
cord-009318-zt1o1bcz	226	10	initiation	initiation	NN
cord-009318-zt1o1bcz	226	11	of	of	IN
cord-009318-zt1o1bcz	226	12	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	226	13	(	(	-LRB-
cord-009318-zt1o1bcz	226	14	23	23	CD
cord-009318-zt1o1bcz	226	15	,	,	,
cord-009318-zt1o1bcz	226	16	(	(	-LRB-
cord-009318-zt1o1bcz	226	17	38	38	CD
cord-009318-zt1o1bcz	226	18	)	)	-RRB-
cord-009318-zt1o1bcz	226	19	(	(	-LRB-
cord-009318-zt1o1bcz	226	20	39	39	CD
cord-009318-zt1o1bcz	226	21	)	)	-RRB-
cord-009318-zt1o1bcz	226	22	.	.	.
cord-009318-zt1o1bcz	227	1	Further	further	RB
cord-009318-zt1o1bcz	227	2	,	,	,
cord-009318-zt1o1bcz	227	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	227	4	observed	observe	VBD
cord-009318-zt1o1bcz	227	5	some	some	DT
cord-009318-zt1o1bcz	227	6	variability	variability	NN
cord-009318-zt1o1bcz	227	7	in	in	IN
cord-009318-zt1o1bcz	227	8	the	the	DT
cord-009318-zt1o1bcz	227	9	maximum	maximum	JJ
cord-009318-zt1o1bcz	227	10	rate	rate	NN
cord-009318-zt1o1bcz	227	11	despite	despite	IN
cord-009318-zt1o1bcz	227	12	similar	similar	JJ
cord-009318-zt1o1bcz	227	13	T	T	NNP
cord-009318-zt1o1bcz	227	14	m	m	NN
cord-009318-zt1o1bcz	227	15	(	(	-LRB-
cord-009318-zt1o1bcz	227	16	23.7	23.7	CD
cord-009318-zt1o1bcz	227	17	±	±	CD
cord-009318-zt1o1bcz	227	18	6.8	6.8	CD
cord-009318-zt1o1bcz	227	19	RFU/30	RFU/30	NNS
cord-009318-zt1o1bcz	227	20	s	s	NNP
cord-009318-zt1o1bcz	227	21	,	,	,
cord-009318-zt1o1bcz	227	22	with	with	IN
cord-009318-zt1o1bcz	227	23	87.5	87.5	CD
cord-009318-zt1o1bcz	227	24	±	±	NNP
cord-009318-zt1o1bcz	227	25	0.2	0.2	CD
cord-009318-zt1o1bcz	227	26	•	•	NN
cord-009318-zt1o1bcz	227	27	C	c	NN
cord-009318-zt1o1bcz	227	28	T	t	NN
cord-009318-zt1o1bcz	227	29	m	m	NN
cord-009318-zt1o1bcz	227	30	)	)	-RRB-
cord-009318-zt1o1bcz	227	31	,	,	,
cord-009318-zt1o1bcz	227	32	which	which	WDT
cord-009318-zt1o1bcz	227	33	indicates	indicate	VBZ
cord-009318-zt1o1bcz	227	34	the	the	DT
cord-009318-zt1o1bcz	227	35	same	same	JJ
cord-009318-zt1o1bcz	227	36	product	product	NN
cord-009318-zt1o1bcz	227	37	may	may	MD
cord-009318-zt1o1bcz	227	38	amplify	amplify	VB
cord-009318-zt1o1bcz	227	39	at	at	IN
cord-009318-zt1o1bcz	227	40	different	different	JJ
cord-009318-zt1o1bcz	227	41	velocities	velocity	NNS
cord-009318-zt1o1bcz	227	42	(	(	-LRB-
cord-009318-zt1o1bcz	227	43	Figure	figure	NN
cord-009318-zt1o1bcz	227	44	5	5	CD
cord-009318-zt1o1bcz	227	45	G	g	NN
cord-009318-zt1o1bcz	227	46	)	)	-RRB-
cord-009318-zt1o1bcz	227	47	.	.	.
cord-009318-zt1o1bcz	228	1	In	in	IN
cord-009318-zt1o1bcz	228	2	general	general	JJ
cord-009318-zt1o1bcz	228	3	,	,	,
cord-009318-zt1o1bcz	228	4	we	-PRON-	PRP
cord-009318-zt1o1bcz	228	5	observed	observe	VBD
cord-009318-zt1o1bcz	228	6	that	that	IN
cord-009318-zt1o1bcz	228	7	maximum	maximum	JJ
cord-009318-zt1o1bcz	228	8	rate	rate	NN
cord-009318-zt1o1bcz	228	9	often	often	RB
cord-009318-zt1o1bcz	228	10	corresponded	correspond	VBD
cord-009318-zt1o1bcz	228	11	to	to	IN
cord-009318-zt1o1bcz	228	12	the	the	DT
cord-009318-zt1o1bcz	228	13	point	point	NN
cord-009318-zt1o1bcz	228	14	when	when	WRB
cord-009318-zt1o1bcz	228	15	the	the	DT
cord-009318-zt1o1bcz	228	16	reaction	reaction	NN
cord-009318-zt1o1bcz	228	17	first	first	RB
cord-009318-zt1o1bcz	228	18	began	begin	VBD
cord-009318-zt1o1bcz	228	19	to	to	TO
cord-009318-zt1o1bcz	228	20	amplify	amplify	VB
cord-009318-zt1o1bcz	228	21	.	.	.
cord-009318-zt1o1bcz	229	1	By	by	IN
cord-009318-zt1o1bcz	229	2	plotting	plot	VBG
cord-009318-zt1o1bcz	229	3	rate	rate	NN
cord-009318-zt1o1bcz	229	4	as	as	IN
cord-009318-zt1o1bcz	229	5	a	a	DT
cord-009318-zt1o1bcz	229	6	function	function	NN
cord-009318-zt1o1bcz	229	7	of	of	IN
cord-009318-zt1o1bcz	229	8	TTP	TTP	NNP
cord-009318-zt1o1bcz	229	9	(	(	-LRB-
cord-009318-zt1o1bcz	229	10	Figure	figure	NN
cord-009318-zt1o1bcz	229	11	 	 	_SP
cord-009318-zt1o1bcz	229	12	5H	5h	NN
cord-009318-zt1o1bcz	229	13	)	)	-RRB-
cord-009318-zt1o1bcz	229	14	we	-PRON-	PRP
cord-009318-zt1o1bcz	229	15	observed	observe	VBD
cord-009318-zt1o1bcz	229	16	little	little	JJ
cord-009318-zt1o1bcz	229	17	fluctuation	fluctuation	NN
cord-009318-zt1o1bcz	229	18	in	in	IN
cord-009318-zt1o1bcz	229	19	rate	rate	NN
cord-009318-zt1o1bcz	229	20	across	across	IN
cord-009318-zt1o1bcz	229	21	a	a	DT
cord-009318-zt1o1bcz	229	22	range	range	NN
cord-009318-zt1o1bcz	229	23	of	of	IN
cord-009318-zt1o1bcz	229	24	different	different	JJ
cord-009318-zt1o1bcz	229	25	TTPs	ttp	NNS
cord-009318-zt1o1bcz	229	26	(	(	-LRB-
cord-009318-zt1o1bcz	229	27	23.7	23.7	CD
cord-009318-zt1o1bcz	229	28	±	±	CD
cord-009318-zt1o1bcz	229	29	6.8	6.8	CD
cord-009318-zt1o1bcz	229	30	RFU/30	RFU/30	NNS
cord-009318-zt1o1bcz	229	31	s	s	NNP
cord-009318-zt1o1bcz	229	32	with	with	IN
cord-009318-zt1o1bcz	229	33	28.6	28.6	CD
cord-009318-zt1o1bcz	229	34	±	±	CD
cord-009318-zt1o1bcz	229	35	8.9	8.9	CD
cord-009318-zt1o1bcz	229	36	min	min	NNP
cord-009318-zt1o1bcz	229	37	)	)	-RRB-
cord-009318-zt1o1bcz	229	38	,	,	,
cord-009318-zt1o1bcz	229	39	indicating	indicate	VBG
cord-009318-zt1o1bcz	229	40	that	that	IN
cord-009318-zt1o1bcz	229	41	the	the	DT
cord-009318-zt1o1bcz	229	42	differences	difference	NNS
cord-009318-zt1o1bcz	229	43	in	in	IN
cord-009318-zt1o1bcz	229	44	TTP	TTP	NNP
cord-009318-zt1o1bcz	229	45	are	be	VBP
cord-009318-zt1o1bcz	229	46	mostly	mostly	RB
cord-009318-zt1o1bcz	229	47	delays	delay	NNS
cord-009318-zt1o1bcz	229	48	in	in	IN
cord-009318-zt1o1bcz	229	49	the	the	DT
cord-009318-zt1o1bcz	229	50	initiation	initiation	NN
cord-009318-zt1o1bcz	229	51	of	of	IN
cord-009318-zt1o1bcz	229	52	amplification	amplification	NN
cord-009318-zt1o1bcz	229	53	rather	rather	RB
cord-009318-zt1o1bcz	229	54	than	than	IN
cord-009318-zt1o1bcz	229	55	differences	difference	NNS
cord-009318-zt1o1bcz	229	56	in	in	IN
cord-009318-zt1o1bcz	229	57	the	the	DT
cord-009318-zt1o1bcz	229	58	rate	rate	NN
cord-009318-zt1o1bcz	229	59	of	of	IN
cord-009318-zt1o1bcz	229	60	amplification	amplification	NN
cord-009318-zt1o1bcz	229	61	.	.	.
cord-009318-zt1o1bcz	230	1	The	the	DT
cord-009318-zt1o1bcz	230	2	use	use	NN
cord-009318-zt1o1bcz	230	3	of	of	IN
cord-009318-zt1o1bcz	230	4	real	real	JJ
cord-009318-zt1o1bcz	230	5	-	-	HYPH
cord-009318-zt1o1bcz	230	6	time	time	NN
cord-009318-zt1o1bcz	230	7	data	datum	NNS
cord-009318-zt1o1bcz	230	8	revealed	reveal	VBD
cord-009318-zt1o1bcz	230	9	heterogeneity	heterogeneity	NN
cord-009318-zt1o1bcz	230	10	in	in	IN
cord-009318-zt1o1bcz	230	11	the	the	DT
cord-009318-zt1o1bcz	230	12	timing	timing	NN
cord-009318-zt1o1bcz	230	13	of	of	IN
cord-009318-zt1o1bcz	230	14	amplification	amplification	NN
cord-009318-zt1o1bcz	230	15	initiation	initiation	NN
cord-009318-zt1o1bcz	230	16	and	and	CC
cord-009318-zt1o1bcz	230	17	the	the	DT
cord-009318-zt1o1bcz	230	18	amplification	amplification	NN
cord-009318-zt1o1bcz	230	19	rate	rate	NN
cord-009318-zt1o1bcz	230	20	,	,	,
cord-009318-zt1o1bcz	230	21	but	but	CC
cord-009318-zt1o1bcz	230	22	homogeneity	homogeneity	NN
cord-009318-zt1o1bcz	230	23	in	in	IN
cord-009318-zt1o1bcz	230	24	T	T	NNP
cord-009318-zt1o1bcz	230	25	m	m	NNP
cord-009318-zt1o1bcz	230	26	,	,	,
cord-009318-zt1o1bcz	230	27	indicating	indicate	VBG
cord-009318-zt1o1bcz	230	28	stochasticity	stochasticity	NN
cord-009318-zt1o1bcz	230	29	in	in	IN
cord-009318-zt1o1bcz	230	30	initiation	initiation	NN
cord-009318-zt1o1bcz	230	31	of	of	IN
cord-009318-zt1o1bcz	230	32	amplification	amplification	NN
cord-009318-zt1o1bcz	230	33	.	.	.
cord-009318-zt1o1bcz	231	1	In	in	IN
cord-009318-zt1o1bcz	231	2	some	some	DT
cord-009318-zt1o1bcz	231	3	cases	case	NNS
cord-009318-zt1o1bcz	231	4	,	,	,
cord-009318-zt1o1bcz	231	5	outlier	outlier	JJ
cord-009318-zt1o1bcz	231	6	data	data	NN
cord-009318-zt1o1bcz	231	7	points	point	NNS
cord-009318-zt1o1bcz	231	8	for	for	IN
cord-009318-zt1o1bcz	231	9	rate	rate	NN
cord-009318-zt1o1bcz	231	10	occurred	occur	VBD
cord-009318-zt1o1bcz	231	11	.	.	.
cord-009318-zt1o1bcz	232	1	To	to	TO
cord-009318-zt1o1bcz	232	2	determine	determine	VB
cord-009318-zt1o1bcz	232	3	whether	whether	IN
cord-009318-zt1o1bcz	232	4	removing	remove	VBG
cord-009318-zt1o1bcz	232	5	these	these	DT
cord-009318-zt1o1bcz	232	6	outliers	outlier	NNS
cord-009318-zt1o1bcz	232	7	impacted	impact	VBD
cord-009318-zt1o1bcz	232	8	the	the	DT
cord-009318-zt1o1bcz	232	9	distribution	distribution	NN
cord-009318-zt1o1bcz	232	10	of	of	IN
cord-009318-zt1o1bcz	232	11	enzymatic	enzymatic	JJ
cord-009318-zt1o1bcz	232	12	rates	rate	NNS
cord-009318-zt1o1bcz	232	13	,	,	,
cord-009318-zt1o1bcz	232	14	we	-PRON-	PRP
cord-009318-zt1o1bcz	232	15	performed	perform	VBD
cord-009318-zt1o1bcz	232	16	a	a	DT
cord-009318-zt1o1bcz	232	17	non	non	JJ
cord-009318-zt1o1bcz	232	18	-	-	JJ
cord-009318-zt1o1bcz	232	19	parametric	parametric	JJ
cord-009318-zt1o1bcz	232	20	test	test	NN
cord-009318-zt1o1bcz	232	21	(	(	-LRB-
cord-009318-zt1o1bcz	232	22	Supplementary	supplementary	JJ
cord-009318-zt1o1bcz	232	23	Figure	Figure	NNP
cord-009318-zt1o1bcz	232	24	S7	S7	NNP
cord-009318-zt1o1bcz	232	25	)	)	-RRB-
cord-009318-zt1o1bcz	232	26	and	and	CC
cord-009318-zt1o1bcz	232	27	found	find	VBD
cord-009318-zt1o1bcz	232	28	no	no	DT
cord-009318-zt1o1bcz	232	29	significant	significant	JJ
cord-009318-zt1o1bcz	232	30	differences	difference	NNS
cord-009318-zt1o1bcz	232	31	in	in	IN
cord-009318-zt1o1bcz	232	32	enzymatic	enzymatic	JJ
cord-009318-zt1o1bcz	232	33	rates	rate	NNS
cord-009318-zt1o1bcz	232	34	when	when	WRB
cord-009318-zt1o1bcz	232	35	these	these	DT
cord-009318-zt1o1bcz	232	36	outliers	outlier	NNS
cord-009318-zt1o1bcz	232	37	were	be	VBD
cord-009318-zt1o1bcz	232	38	excluded	exclude	VBN
cord-009318-zt1o1bcz	232	39	.	.	.
cord-009318-zt1o1bcz	233	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	233	2	next	next	RB
cord-009318-zt1o1bcz	233	3	asked	ask	VBD
cord-009318-zt1o1bcz	233	4	whether	whether	IN
cord-009318-zt1o1bcz	233	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	233	6	could	could	MD
cord-009318-zt1o1bcz	233	7	observe	observe	VB
cord-009318-zt1o1bcz	233	8	in	in	IN
cord-009318-zt1o1bcz	233	9	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	233	10	the	the	DT
cord-009318-zt1o1bcz	233	11	same	same	JJ
cord-009318-zt1o1bcz	233	12	pattern	pattern	NN
cord-009318-zt1o1bcz	233	13	of	of	IN
cord-009318-zt1o1bcz	233	14	high	high	JJ
cord-009318-zt1o1bcz	233	15	-	-	HYPH
cord-009318-zt1o1bcz	233	16	T	t	NN
cord-009318-zt1o1bcz	233	17	m	m	NN
cord-009318-zt1o1bcz	233	18	non	non	JJ
cord-009318-zt1o1bcz	233	19	-	-	JJ
cord-009318-zt1o1bcz	233	20	specific	specific	JJ
cord-009318-zt1o1bcz	233	21	amplification	amplification	NN
cord-009318-zt1o1bcz	233	22	and	and	CC
cord-009318-zt1o1bcz	233	23	low	low	JJ
cord-009318-zt1o1bcz	233	24	-	-	HYPH
cord-009318-zt1o1bcz	233	25	T	t	NN
cord-009318-zt1o1bcz	233	26	m	m	NN
cord-009318-zt1o1bcz	233	27	specific	specific	JJ
cord-009318-zt1o1bcz	233	28	amplification	amplification	NN
cord-009318-zt1o1bcz	233	29	that	that	WDT
cord-009318-zt1o1bcz	233	30	we	-PRON-	PRP
cord-009318-zt1o1bcz	233	31	observed	observe	VBD
cord-009318-zt1o1bcz	233	32	in	in	IN
cord-009318-zt1o1bcz	233	33	bulk	bulk	NN
cord-009318-zt1o1bcz	233	34	.	.	.
cord-009318-zt1o1bcz	234	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	234	2	performed	perform	VBD
cord-009318-zt1o1bcz	234	3	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	234	4	using	use	VBG
cord-009318-zt1o1bcz	234	5	three	three	CD
cord-009318-zt1o1bcz	234	6	chips	chip	NNS
cord-009318-zt1o1bcz	234	7	containing	contain	VBG
cord-009318-zt1o1bcz	234	8	tem-	tem-	DT
cord-009318-zt1o1bcz	234	9	plate	plate	NN
cord-009318-zt1o1bcz	234	10	,	,	,
cord-009318-zt1o1bcz	234	11	and	and	CC
cord-009318-zt1o1bcz	234	12	three	three	CD
cord-009318-zt1o1bcz	234	13	chips	chip	NNS
cord-009318-zt1o1bcz	234	14	lacking	lack	VBG
cord-009318-zt1o1bcz	234	15	template	template	NN
cord-009318-zt1o1bcz	234	16	(	(	-LRB-
cord-009318-zt1o1bcz	234	17	NTC	NTC	NNP
cord-009318-zt1o1bcz	234	18	)	)	-RRB-
cord-009318-zt1o1bcz	234	19	and	and	CC
cord-009318-zt1o1bcz	234	20	observed	observe	VBD
cord-009318-zt1o1bcz	234	21	∼55,000	∼55,000	NN
cord-009318-zt1o1bcz	234	22	partitions	partition	NNS
cord-009318-zt1o1bcz	234	23	for	for	IN
cord-009318-zt1o1bcz	234	24	each	each	DT
cord-009318-zt1o1bcz	234	25	condition	condition	NN
cord-009318-zt1o1bcz	234	26	.	.	.
cord-009318-zt1o1bcz	235	1	Although	although	IN
cord-009318-zt1o1bcz	235	2	60	60	CD
cord-009318-zt1o1bcz	235	3	000	000	CD
cord-009318-zt1o1bcz	235	4	partitions	partition	NNS
cord-009318-zt1o1bcz	235	5	are	be	VBP
cord-009318-zt1o1bcz	235	6	possible	possible	JJ
cord-009318-zt1o1bcz	235	7	,	,	,
cord-009318-zt1o1bcz	235	8	not	not	RB
cord-009318-zt1o1bcz	235	9	all	all	DT
cord-009318-zt1o1bcz	235	10	partitions	partition	NNS
cord-009318-zt1o1bcz	235	11	filled	fill	VBN
cord-009318-zt1o1bcz	235	12	nor	nor	CC
cord-009318-zt1o1bcz	235	13	can	can	MD
cord-009318-zt1o1bcz	235	14	all	all	DT
cord-009318-zt1o1bcz	235	15	partitions	partition	NNS
cord-009318-zt1o1bcz	235	16	be	be	VB
cord-009318-zt1o1bcz	235	17	tracked	track	VBN
cord-009318-zt1o1bcz	235	18	for	for	IN
cord-009318-zt1o1bcz	235	19	the	the	DT
cord-009318-zt1o1bcz	235	20	full	full	JJ
cord-009318-zt1o1bcz	235	21	duration	duration	NN
cord-009318-zt1o1bcz	235	22	of	of	IN
cord-009318-zt1o1bcz	235	23	an	an	DT
cord-009318-zt1o1bcz	235	24	experiment	experiment	NN
cord-009318-zt1o1bcz	235	25	.	.	.
cord-009318-zt1o1bcz	236	1	For	for	IN
cord-009318-zt1o1bcz	236	2	the	the	DT
cord-009318-zt1o1bcz	236	3	melt	melt	NN
cord-009318-zt1o1bcz	236	4	curve	curve	NN
cord-009318-zt1o1bcz	236	5	,	,	,
cord-009318-zt1o1bcz	236	6	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	236	7	readings	reading	NNS
cord-009318-zt1o1bcz	236	8	were	be	VBD
cord-009318-zt1o1bcz	236	9	taken	take	VBN
cord-009318-zt1o1bcz	236	10	at	at	IN
cord-009318-zt1o1bcz	236	11	1	1	CD
cord-009318-zt1o1bcz	236	12	•	•	NN
cord-009318-zt1o1bcz	237	1	C	C	NNP
cord-009318-zt1o1bcz	237	2	increments	increment	NNS
cord-009318-zt1o1bcz	237	3	from	from	IN
cord-009318-zt1o1bcz	237	4	55	55	CD
cord-009318-zt1o1bcz	237	5	-	-	SYM
cord-009318-zt1o1bcz	237	6	90	90	CD
cord-009318-zt1o1bcz	237	7	•	•	NN
cord-009318-zt1o1bcz	237	8	C	c	NN
cord-009318-zt1o1bcz	237	9	;	;	:
cord-009318-zt1o1bcz	237	10	and	and	CC
cord-009318-zt1o1bcz	237	11	at	at	IN
cord-009318-zt1o1bcz	237	12	0.5	0.5	CD
cord-009318-zt1o1bcz	238	1	•	•	NNP
cord-009318-zt1o1bcz	239	1	C	C	NNP
cord-009318-zt1o1bcz	239	2	increments	increment	NNS
cord-009318-zt1o1bcz	239	3	from	from	IN
cord-009318-zt1o1bcz	239	4	90	90	CD
cord-009318-zt1o1bcz	239	5	-	-	SYM
cord-009318-zt1o1bcz	239	6	95	95	CD
cord-009318-zt1o1bcz	240	1	•	•	NNP
cord-009318-zt1o1bcz	240	2	C	C	NNP
cord-009318-zt1o1bcz	240	3	to	to	TO
cord-009318-zt1o1bcz	240	4	give	give	VB
cord-009318-zt1o1bcz	240	5	higher	high	JJR
cord-009318-zt1o1bcz	240	6	resolution	resolution	NN
cord-009318-zt1o1bcz	240	7	.	.	.
cord-009318-zt1o1bcz	241	1	Due	due	IN
cord-009318-zt1o1bcz	241	2	to	to	IN
cord-009318-zt1o1bcz	241	3	slight	slight	JJ
cord-009318-zt1o1bcz	241	4	differences	difference	NNS
cord-009318-zt1o1bcz	241	5	in	in	IN
cord-009318-zt1o1bcz	241	6	the	the	DT
cord-009318-zt1o1bcz	241	7	timing	timing	NN
cord-009318-zt1o1bcz	241	8	between	between	IN
cord-009318-zt1o1bcz	241	9	the	the	DT
cord-009318-zt1o1bcz	241	10	heating	heating	NN
cord-009318-zt1o1bcz	241	11	element	element	NN
cord-009318-zt1o1bcz	241	12	and	and	CC
cord-009318-zt1o1bcz	241	13	the	the	DT
cord-009318-zt1o1bcz	241	14	image	image	NN
cord-009318-zt1o1bcz	241	15	collection	collection	NN
cord-009318-zt1o1bcz	241	16	,	,	,
cord-009318-zt1o1bcz	241	17	some	some	DT
cord-009318-zt1o1bcz	241	18	chips	chip	NNS
cord-009318-zt1o1bcz	241	19	were	be	VBD
cord-009318-zt1o1bcz	241	20	observed	observe	VBN
cord-009318-zt1o1bcz	241	21	at	at	IN
cord-009318-zt1o1bcz	241	22	slightly	slightly	RB
cord-009318-zt1o1bcz	241	23	different	different	JJ
cord-009318-zt1o1bcz	241	24	temperatures	temperature	NNS
cord-009318-zt1o1bcz	241	25	(	(	-LRB-
cord-009318-zt1o1bcz	242	1	<	<	XX
cord-009318-zt1o1bcz	242	2	0.5	0.5	CD
cord-009318-zt1o1bcz	242	3	•	•	NN
cord-009318-zt1o1bcz	242	4	C	c	NN
cord-009318-zt1o1bcz	242	5	)	)	-RRB-
cord-009318-zt1o1bcz	242	6	.	.	.
cord-009318-zt1o1bcz	243	1	Our	-PRON-	PRP$
cord-009318-zt1o1bcz	243	2	approach	approach	NN
cord-009318-zt1o1bcz	243	3	enabled	enable	VBD
cord-009318-zt1o1bcz	243	4	us	-PRON-	PRP
cord-009318-zt1o1bcz	243	5	to	to	TO
cord-009318-zt1o1bcz	243	6	differentiate	differentiate	VB
cord-009318-zt1o1bcz	243	7	specific	specific	JJ
cord-009318-zt1o1bcz	243	8	and	and	CC
cord-009318-zt1o1bcz	243	9	non	non	JJ
cord-009318-zt1o1bcz	243	10	-	-	JJ
cord-009318-zt1o1bcz	243	11	specific	specific	JJ
cord-009318-zt1o1bcz	243	12	amplification	amplification	NN
cord-009318-zt1o1bcz	243	13	events	event	NNS
cord-009318-zt1o1bcz	243	14	using	use	VBG
cord-009318-zt1o1bcz	243	15	HRM	HRM	NNP
cord-009318-zt1o1bcz	243	16	.	.	.
cord-009318-zt1o1bcz	244	1	When	when	WRB
cord-009318-zt1o1bcz	244	2	using	use	VBG
cord-009318-zt1o1bcz	244	3	the	the	DT
cord-009318-zt1o1bcz	244	4	polymerase	polymerase	NN
cord-009318-zt1o1bcz	244	5	Bst	Bst	NNP
cord-009318-zt1o1bcz	244	6	2.0	2.0	NNP
cord-009318-zt1o1bcz	244	7	and	and	CC
cord-009318-zt1o1bcz	244	8	template	template	NN
cord-009318-zt1o1bcz	244	9	(	(	-LRB-
cord-009318-zt1o1bcz	244	10	Figure	figure	NN
cord-009318-zt1o1bcz	244	11	6A	6a	NN
cord-009318-zt1o1bcz	244	12	,	,	,
cord-009318-zt1o1bcz	244	13	blue	blue	JJ
cord-009318-zt1o1bcz	244	14	points	point	NNS
cord-009318-zt1o1bcz	244	15	)	)	-RRB-
cord-009318-zt1o1bcz	244	16	,	,	,
cord-009318-zt1o1bcz	244	17	we	-PRON-	PRP
cord-009318-zt1o1bcz	244	18	observed	observe	VBD
cord-009318-zt1o1bcz	244	19	a	a	DT
cord-009318-zt1o1bcz	244	20	large	large	JJ
cord-009318-zt1o1bcz	244	21	band	band	NN
cord-009318-zt1o1bcz	244	22	of	of	IN
cord-009318-zt1o1bcz	244	23	amplification	amplification	NN
cord-009318-zt1o1bcz	244	24	in	in	IN
cord-009318-zt1o1bcz	244	25	the	the	DT
cord-009318-zt1o1bcz	244	26	temperature	temperature	NN
cord-009318-zt1o1bcz	244	27	range	range	NN
cord-009318-zt1o1bcz	244	28	88.5	88.5	CD
cord-009318-zt1o1bcz	245	1	-90.3	-90.3	DT
cord-009318-zt1o1bcz	245	2	•	•	NNP
cord-009318-zt1o1bcz	245	3	C	C	NNP
cord-009318-zt1o1bcz	245	4	,	,	,
cord-009318-zt1o1bcz	245	5	in	in	IN
cord-009318-zt1o1bcz	245	6	agreement	agreement	NN
cord-009318-zt1o1bcz	245	7	with	with	IN
cord-009318-zt1o1bcz	245	8	the	the	DT
cord-009318-zt1o1bcz	245	9	T	T	NNP
cord-009318-zt1o1bcz	245	10	m	m	NN
cord-009318-zt1o1bcz	245	11	observed	observe	VBD
cord-009318-zt1o1bcz	245	12	when	when	WRB
cord-009318-zt1o1bcz	245	13	performing	perform	VBG
cord-009318-zt1o1bcz	245	14	the	the	DT
cord-009318-zt1o1bcz	245	15	reaction	reaction	NN
cord-009318-zt1o1bcz	245	16	in	in	IN
cord-009318-zt1o1bcz	245	17	bulk	bulk	NN
cord-009318-zt1o1bcz	245	18	(	(	-LRB-
cord-009318-zt1o1bcz	245	19	Figure	figure	NN
cord-009318-zt1o1bcz	245	20	1	1	CD
cord-009318-zt1o1bcz	245	21	)	)	-RRB-
cord-009318-zt1o1bcz	245	22	.	.	.
cord-009318-zt1o1bcz	246	1	In	in	IN
cord-009318-zt1o1bcz	246	2	contrast	contrast	NN
cord-009318-zt1o1bcz	246	3	,	,	,
cord-009318-zt1o1bcz	246	4	the	the	DT
cord-009318-zt1o1bcz	246	5	NTC	NTC	NNP
cord-009318-zt1o1bcz	246	6	(	(	-LRB-
cord-009318-zt1o1bcz	246	7	Figure	Figure	NNP
cord-009318-zt1o1bcz	246	8	6A	6a	NN
cord-009318-zt1o1bcz	246	9	,	,	,
cord-009318-zt1o1bcz	246	10	red	red	JJ
cord-009318-zt1o1bcz	246	11	points	point	NNS
cord-009318-zt1o1bcz	246	12	)	)	-RRB-
cord-009318-zt1o1bcz	246	13	had	have	VBD
cord-009318-zt1o1bcz	246	14	very	very	RB
cord-009318-zt1o1bcz	246	15	few	few	JJ
cord-009318-zt1o1bcz	246	16	amplification	amplification	NN
cord-009318-zt1o1bcz	246	17	events	event	NNS
cord-009318-zt1o1bcz	246	18	in	in	IN
cord-009318-zt1o1bcz	246	19	that	that	DT
cord-009318-zt1o1bcz	246	20	temperature	temperature	NN
cord-009318-zt1o1bcz	246	21	range	range	NN
cord-009318-zt1o1bcz	246	22	(	(	-LRB-
cord-009318-zt1o1bcz	246	23	68	68	CD
cord-009318-zt1o1bcz	246	24	out	out	IN
cord-009318-zt1o1bcz	246	25	of	of	IN
cord-009318-zt1o1bcz	246	26	51	51	CD
cord-009318-zt1o1bcz	246	27	279	279	CD
cord-009318-zt1o1bcz	246	28	partitions	partition	NNS
cord-009318-zt1o1bcz	246	29	)	)	-RRB-
cord-009318-zt1o1bcz	246	30	.	.	.
cord-009318-zt1o1bcz	247	1	Hence	hence	RB
cord-009318-zt1o1bcz	247	2	,	,	,
cord-009318-zt1o1bcz	247	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	247	4	defined	define	VBD
cord-009318-zt1o1bcz	247	5	events	event	NNS
cord-009318-zt1o1bcz	247	6	that	that	WDT
cord-009318-zt1o1bcz	247	7	occurred	occur	VBD
cord-009318-zt1o1bcz	247	8	in	in	IN
cord-009318-zt1o1bcz	247	9	the	the	DT
cord-009318-zt1o1bcz	247	10	T	t	NN
cord-009318-zt1o1bcz	247	11	m	m	NNP
cord-009318-zt1o1bcz	247	12	range	range	NNP
cord-009318-zt1o1bcz	247	13	88.5	88.5	CD
cord-009318-zt1o1bcz	247	14	-	-	HYPH
cord-009318-zt1o1bcz	247	15	90.3	90.3	CD
cord-009318-zt1o1bcz	247	16	•	•	NN
cord-009318-zt1o1bcz	247	17	C	C	NNP
cord-009318-zt1o1bcz	247	18	as	as	IN
cord-009318-zt1o1bcz	247	19	true	true	JJ
cord-009318-zt1o1bcz	247	20	positives	positive	NNS
cord-009318-zt1o1bcz	247	21	(	(	-LRB-
cord-009318-zt1o1bcz	247	22	specific	specific	JJ
cord-009318-zt1o1bcz	247	23	amplification	amplification	NN
cord-009318-zt1o1bcz	247	24	events	event	NNS
cord-009318-zt1o1bcz	247	25	)	)	-RRB-
cord-009318-zt1o1bcz	247	26	and	and	CC
cord-009318-zt1o1bcz	247	27	we	-PRON-	PRP
cord-009318-zt1o1bcz	247	28	defined	define	VBD
cord-009318-zt1o1bcz	247	29	those	those	DT
cord-009318-zt1o1bcz	247	30	that	that	WDT
cord-009318-zt1o1bcz	247	31	occurred	occur	VBD
cord-009318-zt1o1bcz	247	32	outside	outside	IN
cord-009318-zt1o1bcz	247	33	this	this	DT
cord-009318-zt1o1bcz	247	34	range	range	NN
cord-009318-zt1o1bcz	247	35	(	(	-LRB-
cord-009318-zt1o1bcz	247	36	in	in	IN
cord-009318-zt1o1bcz	247	37	both	both	CC
cord-009318-zt1o1bcz	247	38	the	the	DT
cord-009318-zt1o1bcz	247	39	NTC	NTC	NNP
cord-009318-zt1o1bcz	247	40	and	and	CC
cord-009318-zt1o1bcz	247	41	in	in	IN
cord-009318-zt1o1bcz	247	42	the	the	DT
cord-009318-zt1o1bcz	247	43	presence	presence	NN
cord-009318-zt1o1bcz	247	44	of	of	IN
cord-009318-zt1o1bcz	247	45	template	template	NN
cord-009318-zt1o1bcz	247	46	)	)	-RRB-
cord-009318-zt1o1bcz	247	47	as	as	IN
cord-009318-zt1o1bcz	247	48	false	false	JJ
cord-009318-zt1o1bcz	247	49	positives	positive	NNS
cord-009318-zt1o1bcz	247	50	(	(	-LRB-
cord-009318-zt1o1bcz	247	51	non	non	JJ
cord-009318-zt1o1bcz	247	52	-	-	JJ
cord-009318-zt1o1bcz	247	53	specific	specific	JJ
cord-009318-zt1o1bcz	247	54	amplification	amplification	NN
cord-009318-zt1o1bcz	247	55	events	event	NNS
cord-009318-zt1o1bcz	247	56	)	)	-RRB-
cord-009318-zt1o1bcz	247	57	.	.	.
cord-009318-zt1o1bcz	248	1	When	when	WRB
cord-009318-zt1o1bcz	248	2	using	use	VBG
cord-009318-zt1o1bcz	248	3	the	the	DT
cord-009318-zt1o1bcz	248	4	polymerase	polymerase	NN
cord-009318-zt1o1bcz	248	5	Bst	Bst	NNP
cord-009318-zt1o1bcz	248	6	3.0	3.0	NNP
cord-009318-zt1o1bcz	248	7	,	,	,
cord-009318-zt1o1bcz	248	8	we	-PRON-	PRP
cord-009318-zt1o1bcz	248	9	observed	observe	VBD
cord-009318-zt1o1bcz	248	10	a	a	DT
cord-009318-zt1o1bcz	248	11	large	large	JJ
cord-009318-zt1o1bcz	248	12	band	band	NN
cord-009318-zt1o1bcz	248	13	of	of	IN
cord-009318-zt1o1bcz	248	14	amplification	amplification	NN
cord-009318-zt1o1bcz	248	15	from	from	IN
cord-009318-zt1o1bcz	248	16	91.25	91.25	CD
cord-009318-zt1o1bcz	248	17	to	to	IN
cord-009318-zt1o1bcz	248	18	92.75	92.75	CD
cord-009318-zt1o1bcz	248	19	•	•	NN
cord-009318-zt1o1bcz	248	20	C	c	NN
cord-009318-zt1o1bcz	248	21	in	in	IN
cord-009318-zt1o1bcz	248	22	the	the	DT
cord-009318-zt1o1bcz	248	23	presence	presence	NN
cord-009318-zt1o1bcz	248	24	of	of	IN
cord-009318-zt1o1bcz	248	25	template	template	NN
cord-009318-zt1o1bcz	248	26	(	(	-LRB-
cord-009318-zt1o1bcz	248	27	Figure	figure	NN
cord-009318-zt1o1bcz	248	28	6B	6b	NN
cord-009318-zt1o1bcz	248	29	,	,	,
cord-009318-zt1o1bcz	248	30	blue	blue	JJ
cord-009318-zt1o1bcz	248	31	points	point	NNS
cord-009318-zt1o1bcz	248	32	)	)	-RRB-
cord-009318-zt1o1bcz	248	33	that	that	WDT
cord-009318-zt1o1bcz	248	34	did	do	VBD
cord-009318-zt1o1bcz	248	35	not	not	RB
cord-009318-zt1o1bcz	248	36	correspond	correspond	VB
cord-009318-zt1o1bcz	248	37	with	with	IN
cord-009318-zt1o1bcz	248	38	amplification	amplification	NN
cord-009318-zt1o1bcz	248	39	in	in	IN
cord-009318-zt1o1bcz	248	40	the	the	DT
cord-009318-zt1o1bcz	248	41	NTC	NTC	NNP
cord-009318-zt1o1bcz	248	42	(	(	-LRB-
cord-009318-zt1o1bcz	248	43	Figure	Figure	NNP
cord-009318-zt1o1bcz	248	44	6B	6b	NN
cord-009318-zt1o1bcz	248	45	,	,	,
cord-009318-zt1o1bcz	248	46	red	red	JJ
cord-009318-zt1o1bcz	248	47	points	point	NNS
cord-009318-zt1o1bcz	248	48	)	)	-RRB-
cord-009318-zt1o1bcz	249	1	so	so	RB
cord-009318-zt1o1bcz	249	2	we	-PRON-	PRP
cord-009318-zt1o1bcz	249	3	defined	define	VBD
cord-009318-zt1o1bcz	249	4	these	these	DT
cord-009318-zt1o1bcz	249	5	as	as	IN
cord-009318-zt1o1bcz	249	6	specific	specific	JJ
cord-009318-zt1o1bcz	249	7	amplification	amplification	NN
cord-009318-zt1o1bcz	249	8	events	event	NNS
cord-009318-zt1o1bcz	249	9	.	.	.
cord-009318-zt1o1bcz	250	1	As	as	IN
cord-009318-zt1o1bcz	250	2	with	with	IN
cord-009318-zt1o1bcz	250	3	bulk	bulk	JJ
cord-009318-zt1o1bcz	250	4	measurements	measurement	NNS
cord-009318-zt1o1bcz	250	5	,	,	,
cord-009318-zt1o1bcz	250	6	we	-PRON-	PRP
cord-009318-zt1o1bcz	250	7	determined	determine	VBD
cord-009318-zt1o1bcz	250	8	the	the	DT
cord-009318-zt1o1bcz	250	9	difference	difference	NN
cord-009318-zt1o1bcz	250	10	in	in	IN
cord-009318-zt1o1bcz	250	11	T	T	NNP
cord-009318-zt1o1bcz	250	12	m	m	NN
cord-009318-zt1o1bcz	250	13	between	between	IN
cord-009318-zt1o1bcz	250	14	specific	specific	JJ
cord-009318-zt1o1bcz	250	15	amplification	amplification	NN
cord-009318-zt1o1bcz	250	16	events	event	NNS
cord-009318-zt1o1bcz	250	17	between	between	IN
cord-009318-zt1o1bcz	250	18	Bst	Bst	NNP
cord-009318-zt1o1bcz	250	19	2.0	2.0	NNP
cord-009318-zt1o1bcz	250	20	and	and	CC
cord-009318-zt1o1bcz	250	21	Bst	Bst	NNP
cord-009318-zt1o1bcz	250	22	3.0	3.0	NNP
cord-009318-zt1o1bcz	250	23	was	be	VBD
cord-009318-zt1o1bcz	250	24	due	due	JJ
cord-009318-zt1o1bcz	250	25	to	to	IN
cord-009318-zt1o1bcz	250	26	the	the	DT
cord-009318-zt1o1bcz	250	27	difference	difference	NN
cord-009318-zt1o1bcz	250	28	in	in	IN
cord-009318-zt1o1bcz	250	29	buffer	buffer	NN
cord-009318-zt1o1bcz	250	30	composition	composition	NN
cord-009318-zt1o1bcz	250	31	(	(	-LRB-
cord-009318-zt1o1bcz	250	32	Supplementary	supplementary	JJ
cord-009318-zt1o1bcz	250	33	Figure	Figure	NNP
cord-009318-zt1o1bcz	250	34	S1	S1	NNP
cord-009318-zt1o1bcz	250	35	and	and	CC
cord-009318-zt1o1bcz	250	36	Table	Table	NNP
cord-009318-zt1o1bcz	250	37	S1	S1	NNP
cord-009318-zt1o1bcz	250	38	)	)	-RRB-
cord-009318-zt1o1bcz	250	39	.	.	.
cord-009318-zt1o1bcz	251	1	During	during	IN
cord-009318-zt1o1bcz	251	2	these	these	DT
cord-009318-zt1o1bcz	251	3	experiments	experiment	NNS
cord-009318-zt1o1bcz	251	4	,	,	,
cord-009318-zt1o1bcz	251	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	251	6	observed	observe	VBD
cord-009318-zt1o1bcz	251	7	two	two	CD
cord-009318-zt1o1bcz	251	8	common	common	JJ
cord-009318-zt1o1bcz	251	9	patterns	pattern	NNS
cord-009318-zt1o1bcz	251	10	.	.	.
cord-009318-zt1o1bcz	252	1	First	first	RB
cord-009318-zt1o1bcz	252	2	,	,	,
cord-009318-zt1o1bcz	252	3	the	the	DT
cord-009318-zt1o1bcz	252	4	T	t	NN
cord-009318-zt1o1bcz	252	5	m	m	NN
cord-009318-zt1o1bcz	252	6	for	for	IN
cord-009318-zt1o1bcz	252	7	specific	specific	JJ
cord-009318-zt1o1bcz	252	8	amplification	amplification	NN
cord-009318-zt1o1bcz	252	9	events	event	NNS
cord-009318-zt1o1bcz	252	10	was	be	VBD
cord-009318-zt1o1bcz	252	11	3	3	CD
cord-009318-zt1o1bcz	252	12	-	-	SYM
cord-009318-zt1o1bcz	252	13	5	5	CD
cord-009318-zt1o1bcz	252	14	•	•	NN
cord-009318-zt1o1bcz	253	1	C	C	NNP
cord-009318-zt1o1bcz	253	2	lower	lower	RBR
cord-009318-zt1o1bcz	253	3	in	in	IN
cord-009318-zt1o1bcz	253	4	digital	digital	JJ
cord-009318-zt1o1bcz	253	5	compared	compare	VBN
cord-009318-zt1o1bcz	253	6	with	with	IN
cord-009318-zt1o1bcz	253	7	bulk	bulk	JJ
cord-009318-zt1o1bcz	253	8	measurements	measurement	NNS
cord-009318-zt1o1bcz	253	9	.	.	.
cord-009318-zt1o1bcz	254	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	254	2	attribute	attribute	VBP
cord-009318-zt1o1bcz	254	3	this	this	DT
cord-009318-zt1o1bcz	254	4	difference	difference	NN
cord-009318-zt1o1bcz	254	5	to	to	IN
cord-009318-zt1o1bcz	254	6	temperature	temperature	NN
cord-009318-zt1o1bcz	254	7	calibration	calibration	NN
cord-009318-zt1o1bcz	254	8	;	;	:
cord-009318-zt1o1bcz	254	9	the	the	DT
cord-009318-zt1o1bcz	254	10	thermocycler	thermocycler	NN
cord-009318-zt1o1bcz	254	11	is	be	VBZ
cord-009318-zt1o1bcz	254	12	calibrated	calibrate	VBN
cord-009318-zt1o1bcz	254	13	to	to	IN
cord-009318-zt1o1bcz	254	14	the	the	DT
cord-009318-zt1o1bcz	254	15	liquid	liquid	JJ
cord-009318-zt1o1bcz	254	16	temperature	temperature	NN
cord-009318-zt1o1bcz	254	17	,	,	,
cord-009318-zt1o1bcz	254	18	whereas	whereas	IN
cord-009318-zt1o1bcz	254	19	the	the	DT
cord-009318-zt1o1bcz	254	20	thermoelectric	thermoelectric	JJ
cord-009318-zt1o1bcz	254	21	element	element	NN
cord-009318-zt1o1bcz	254	22	measures	measure	VBZ
cord-009318-zt1o1bcz	254	23	the	the	DT
cord-009318-zt1o1bcz	254	24	temperature	temperature	NN
cord-009318-zt1o1bcz	254	25	of	of	IN
cord-009318-zt1o1bcz	254	26	the	the	DT
cord-009318-zt1o1bcz	254	27	heating	heating	NN
cord-009318-zt1o1bcz	254	28	element	element	NN
cord-009318-zt1o1bcz	254	29	.	.	.
cord-009318-zt1o1bcz	255	1	Second	second	JJ
cord-009318-zt1o1bcz	255	2	,	,	,
cord-009318-zt1o1bcz	255	3	false	false	JJ
cord-009318-zt1o1bcz	255	4	positives	positive	NNS
cord-009318-zt1o1bcz	255	5	in	in	IN
cord-009318-zt1o1bcz	255	6	the	the	DT
cord-009318-zt1o1bcz	255	7	NTC	NTC	NNP
cord-009318-zt1o1bcz	255	8	had	have	VBD
cord-009318-zt1o1bcz	255	9	predominantly	predominantly	RB
cord-009318-zt1o1bcz	255	10	high	high	JJ
cord-009318-zt1o1bcz	255	11	T	t	NN
cord-009318-zt1o1bcz	255	12	m	m	NN
cord-009318-zt1o1bcz	255	13	,	,	,
cord-009318-zt1o1bcz	255	14	which	which	WDT
cord-009318-zt1o1bcz	255	15	we	-PRON-	PRP
cord-009318-zt1o1bcz	255	16	attribute	attribute	VBP
cord-009318-zt1o1bcz	255	17	to	to	IN
cord-009318-zt1o1bcz	255	18	the	the	DT
cord-009318-zt1o1bcz	255	19	non	non	JJ
cord-009318-zt1o1bcz	255	20	-	-	JJ
cord-009318-zt1o1bcz	255	21	specific	specific	JJ
cord-009318-zt1o1bcz	255	22	product	product	NN
cord-009318-zt1o1bcz	255	23	we	-PRON-	PRP
cord-009318-zt1o1bcz	255	24	identified	identify	VBD
cord-009318-zt1o1bcz	255	25	in	in	IN
cord-009318-zt1o1bcz	255	26	the	the	DT
cord-009318-zt1o1bcz	255	27	bulk	bulk	JJ
cord-009318-zt1o1bcz	255	28	reactions	reaction	NNS
cord-009318-zt1o1bcz	255	29	.	.	.
cord-009318-zt1o1bcz	256	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	256	2	also	also	RB
cord-009318-zt1o1bcz	256	3	observed	observe	VBD
cord-009318-zt1o1bcz	256	4	differences	difference	NNS
cord-009318-zt1o1bcz	256	5	in	in	IN
cord-009318-zt1o1bcz	256	6	total	total	JJ
cord-009318-zt1o1bcz	256	7	amplification	amplification	NN
cord-009318-zt1o1bcz	256	8	events	event	NNS
cord-009318-zt1o1bcz	256	9	between	between	IN
cord-009318-zt1o1bcz	256	10	the	the	DT
cord-009318-zt1o1bcz	256	11	two	two	CD
cord-009318-zt1o1bcz	256	12	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	256	13	.	.	.
cord-009318-zt1o1bcz	257	1	Assays	assay	NNS
cord-009318-zt1o1bcz	257	2	with	with	IN
cord-009318-zt1o1bcz	257	3	Bst	Bst	NNP
cord-009318-zt1o1bcz	257	4	3.0	3.0	CD
cord-009318-zt1o1bcz	257	5	resulted	result	VBD
cord-009318-zt1o1bcz	257	6	in	in	IN
cord-009318-zt1o1bcz	257	7	substantially	substantially	RB
cord-009318-zt1o1bcz	257	8	more	more	RBR
cord-009318-zt1o1bcz	257	9	non	non	JJ
cord-009318-zt1o1bcz	257	10	-	-	JJ
cord-009318-zt1o1bcz	257	11	specific	specific	JJ
cord-009318-zt1o1bcz	257	12	amplification	amplification	NN
cord-009318-zt1o1bcz	257	13	than	than	IN
cord-009318-zt1o1bcz	257	14	those	those	DT
cord-009318-zt1o1bcz	257	15	with	with	IN
cord-009318-zt1o1bcz	257	16	Bst	Bst	NNP
cord-009318-zt1o1bcz	257	17	2.0	2.0	CD
cord-009318-zt1o1bcz	257	18	and	and	CC
cord-009318-zt1o1bcz	257	19	confirmed	confirm	VBD
cord-009318-zt1o1bcz	257	20	this	this	DT
cord-009318-zt1o1bcz	257	21	was	be	VBD
cord-009318-zt1o1bcz	257	22	not	not	RB
cord-009318-zt1o1bcz	257	23	an	an	DT
cord-009318-zt1o1bcz	257	24	issue	issue	NN
cord-009318-zt1o1bcz	257	25	with	with	IN
cord-009318-zt1o1bcz	257	26	buffer	buffer	NN
cord-009318-zt1o1bcz	257	27	selection	selection	NN
cord-009318-zt1o1bcz	257	28	(	(	-LRB-
cord-009318-zt1o1bcz	257	29	Supplementary	supplementary	JJ
cord-009318-zt1o1bcz	257	30	Figure	Figure	NNP
cord-009318-zt1o1bcz	257	31	S1	S1	NNP
cord-009318-zt1o1bcz	257	32	and	and	CC
cord-009318-zt1o1bcz	257	33	Table	Table	NNP
cord-009318-zt1o1bcz	257	34	S1	S1	NNP
cord-009318-zt1o1bcz	257	35	)	)	-RRB-
cord-009318-zt1o1bcz	257	36	.	.	.
cord-009318-zt1o1bcz	258	1	After	after	IN
cord-009318-zt1o1bcz	258	2	90	90	CD
cord-009318-zt1o1bcz	258	3	min	min	NN
cord-009318-zt1o1bcz	258	4	,	,	,
cord-009318-zt1o1bcz	258	5	Next	Next	NNP
cord-009318-zt1o1bcz	258	6	,	,	,
cord-009318-zt1o1bcz	258	7	we	-PRON-	PRP
cord-009318-zt1o1bcz	258	8	tested	test	VBD
cord-009318-zt1o1bcz	258	9	whether	whether	IN
cord-009318-zt1o1bcz	258	10	TTP	TTP	NNP
cord-009318-zt1o1bcz	258	11	is	be	VBZ
cord-009318-zt1o1bcz	258	12	different	different	JJ
cord-009318-zt1o1bcz	258	13	for	for	IN
cord-009318-zt1o1bcz	258	14	specific	specific	JJ
cord-009318-zt1o1bcz	258	15	and	and	CC
cord-009318-zt1o1bcz	258	16	non	non	JJ
cord-009318-zt1o1bcz	258	17	-	-	JJ
cord-009318-zt1o1bcz	258	18	specific	specific	JJ
cord-009318-zt1o1bcz	258	19	amplification	amplification	NN
cord-009318-zt1o1bcz	258	20	.	.	.
cord-009318-zt1o1bcz	259	1	Because	because	IN
cord-009318-zt1o1bcz	259	2	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	259	3	follows	follow	VBZ
cord-009318-zt1o1bcz	259	4	a	a	DT
cord-009318-zt1o1bcz	259	5	'	'	``
cord-009318-zt1o1bcz	259	6	winner	winner	NN
cord-009318-zt1o1bcz	259	7	-	-	HYPH
cord-009318-zt1o1bcz	259	8	takes	take	VBZ
cord-009318-zt1o1bcz	259	9	-	-	HYPH
cord-009318-zt1o1bcz	259	10	all	all	DT
cord-009318-zt1o1bcz	259	11	'	'	''
cord-009318-zt1o1bcz	259	12	format	format	NN
cord-009318-zt1o1bcz	259	13	,	,	,
cord-009318-zt1o1bcz	259	14	frequent	frequent	JJ
cord-009318-zt1o1bcz	259	15	and	and	CC
cord-009318-zt1o1bcz	259	16	early	early	JJ
cord-009318-zt1o1bcz	259	17	non	non	JJ
cord-009318-zt1o1bcz	259	18	-	-	JJ
cord-009318-zt1o1bcz	259	19	specific	specific	JJ
cord-009318-zt1o1bcz	259	20	amplification	amplification	NN
cord-009318-zt1o1bcz	259	21	events	event	NNS
cord-009318-zt1o1bcz	259	22	may	may	MD
cord-009318-zt1o1bcz	259	23	dominate	dominate	VB
cord-009318-zt1o1bcz	259	24	bulk	bulk	JJ
cord-009318-zt1o1bcz	259	25	amplification	amplification	NN
cord-009318-zt1o1bcz	259	26	.	.	.
cord-009318-zt1o1bcz	260	1	In	in	IN
cord-009318-zt1o1bcz	260	2	general	general	JJ
cord-009318-zt1o1bcz	260	3	,	,	,
cord-009318-zt1o1bcz	260	4	for	for	IN
cord-009318-zt1o1bcz	260	5	both	both	DT
cord-009318-zt1o1bcz	260	6	Bst	Bst	NNP
cord-009318-zt1o1bcz	260	7	2.0	2.0	NNP
cord-009318-zt1o1bcz	260	8	and	and	CC
cord-009318-zt1o1bcz	260	9	Bst	Bst	NNP
cord-009318-zt1o1bcz	260	10	3.0	3.0	NNP
cord-009318-zt1o1bcz	260	11	,	,	,
cord-009318-zt1o1bcz	260	12	specific	specific	JJ
cord-009318-zt1o1bcz	260	13	amplification	amplification	NN
cord-009318-zt1o1bcz	260	14	had	have	VBD
cord-009318-zt1o1bcz	260	15	earlier	early	JJR
cord-009318-zt1o1bcz	260	16	TTP	ttp	NN
cord-009318-zt1o1bcz	260	17	than	than	IN
cord-009318-zt1o1bcz	260	18	non	non	JJ
cord-009318-zt1o1bcz	260	19	-	-	JJ
cord-009318-zt1o1bcz	260	20	specific	specific	JJ
cord-009318-zt1o1bcz	260	21	amplification	amplification	NN
cord-009318-zt1o1bcz	260	22	,	,	,
cord-009318-zt1o1bcz	260	23	although	although	IN
cord-009318-zt1o1bcz	260	24	there	there	EX
cord-009318-zt1o1bcz	260	25	was	be	VBD
cord-009318-zt1o1bcz	260	26	some	some	DT
cord-009318-zt1o1bcz	260	27	overlap	overlap	NN
cord-009318-zt1o1bcz	260	28	,	,	,
cord-009318-zt1o1bcz	260	29	mostly	mostly	RB
cord-009318-zt1o1bcz	260	30	>	>	XX
cord-009318-zt1o1bcz	260	31	90.5	90.5	CD
cord-009318-zt1o1bcz	260	32	•	•	NN
cord-009318-zt1o1bcz	260	33	C	c	NN
cord-009318-zt1o1bcz	260	34	(	(	-LRB-
cord-009318-zt1o1bcz	260	35	Figure	figure	NN
cord-009318-zt1o1bcz	260	36	6A	6a	NN
cord-009318-zt1o1bcz	260	37	and	and	CC
cord-009318-zt1o1bcz	260	38	B	b	NN
cord-009318-zt1o1bcz	260	39	)	)	-RRB-
cord-009318-zt1o1bcz	260	40	.	.	.
cord-009318-zt1o1bcz	261	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	261	2	were	be	VBD
cord-009318-zt1o1bcz	261	3	able	able	JJ
cord-009318-zt1o1bcz	261	4	to	to	TO
cord-009318-zt1o1bcz	261	5	distinguish	distinguish	VB
cord-009318-zt1o1bcz	261	6	the	the	DT
cord-009318-zt1o1bcz	261	7	clustering	clustering	NN
cord-009318-zt1o1bcz	261	8	of	of	IN
cord-009318-zt1o1bcz	261	9	high	high	JJ
cord-009318-zt1o1bcz	261	10	-	-	HYPH
cord-009318-zt1o1bcz	261	11	T	t	NN
cord-009318-zt1o1bcz	261	12	m	m	NN
cord-009318-zt1o1bcz	261	13	non	non	JJ
cord-009318-zt1o1bcz	261	14	-	-	JJ
cord-009318-zt1o1bcz	261	15	specific	specific	JJ
cord-009318-zt1o1bcz	261	16	products	product	NNS
cord-009318-zt1o1bcz	261	17	separately	separately	RB
cord-009318-zt1o1bcz	261	18	from	from	IN
cord-009318-zt1o1bcz	261	19	specific	specific	JJ
cord-009318-zt1o1bcz	261	20	amplification	amplification	NN
cord-009318-zt1o1bcz	261	21	using	use	VBG
cord-009318-zt1o1bcz	261	22	a	a	DT
cord-009318-zt1o1bcz	261	23	threshold	threshold	NN
cord-009318-zt1o1bcz	261	24	of	of	IN
cord-009318-zt1o1bcz	261	25	88.5	88.5	CD
cord-009318-zt1o1bcz	261	26	-	-	HYPH
cord-009318-zt1o1bcz	261	27	90.3	90.3	CD
cord-009318-zt1o1bcz	261	28	•	•	NN
cord-009318-zt1o1bcz	261	29	C	c	NN
cord-009318-zt1o1bcz	261	30	(	(	-LRB-
cord-009318-zt1o1bcz	261	31	Figure	figure	NN
cord-009318-zt1o1bcz	261	32	6C	6c	NN
cord-009318-zt1o1bcz	261	33	and	and	CC
cord-009318-zt1o1bcz	261	34	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	261	35	Figure	Figure	NNP
cord-009318-zt1o1bcz	261	36	S8A	s8a	NN
cord-009318-zt1o1bcz	261	37	)	)	-RRB-
cord-009318-zt1o1bcz	261	38	.	.	.
cord-009318-zt1o1bcz	262	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	262	2	illustrate	illustrate	VBP
cord-009318-zt1o1bcz	262	3	each	each	DT
cord-009318-zt1o1bcz	262	4	partition	partition	NN
cord-009318-zt1o1bcz	262	5	with	with	IN
cord-009318-zt1o1bcz	262	6	only	only	JJ
cord-009318-zt1o1bcz	262	7	partial	partial	JJ
cord-009318-zt1o1bcz	262	8	opacity	opacity	NN
cord-009318-zt1o1bcz	262	9	so	so	IN
cord-009318-zt1o1bcz	262	10	that	that	IN
cord-009318-zt1o1bcz	262	11	when	when	WRB
cord-009318-zt1o1bcz	262	12	false	false	JJ
cord-009318-zt1o1bcz	262	13	positives	positive	NNS
cord-009318-zt1o1bcz	262	14	in	in	IN
cord-009318-zt1o1bcz	262	15	the	the	DT
cord-009318-zt1o1bcz	262	16	NTC	NTC	NNP
cord-009318-zt1o1bcz	262	17	(	(	-LRB-
cord-009318-zt1o1bcz	262	18	red	red	NNP
cord-009318-zt1o1bcz	262	19	)	)	-RRB-
cord-009318-zt1o1bcz	262	20	overlap	overlap	VB
cord-009318-zt1o1bcz	262	21	with	with	IN
cord-009318-zt1o1bcz	262	22	false	false	JJ
cord-009318-zt1o1bcz	262	23	positives	positive	NNS
cord-009318-zt1o1bcz	262	24	in	in	IN
cord-009318-zt1o1bcz	262	25	the	the	DT
cord-009318-zt1o1bcz	262	26	template	template	NN
cord-009318-zt1o1bcz	262	27	-	-	HYPH
cord-009318-zt1o1bcz	262	28	containing	contain	VBG
cord-009318-zt1o1bcz	262	29	sample	sample	NN
cord-009318-zt1o1bcz	262	30	(	(	-LRB-
cord-009318-zt1o1bcz	262	31	blue	blue	NNP
cord-009318-zt1o1bcz	262	32	)	)	-RRB-
cord-009318-zt1o1bcz	262	33	,	,	,
cord-009318-zt1o1bcz	262	34	the	the	DT
cord-009318-zt1o1bcz	262	35	overlap	overlap	NN
cord-009318-zt1o1bcz	262	36	of	of	IN
cord-009318-zt1o1bcz	262	37	multiple	multiple	JJ
cord-009318-zt1o1bcz	262	38	colors	color	NNS
cord-009318-zt1o1bcz	262	39	appears	appear	VBZ
cord-009318-zt1o1bcz	262	40	purple	purple	JJ
cord-009318-zt1o1bcz	262	41	(	(	-LRB-
cord-009318-zt1o1bcz	262	42	Figure	figure	NN
cord-009318-zt1o1bcz	262	43	6D	6d	NN
cord-009318-zt1o1bcz	262	44	)	)	-RRB-
cord-009318-zt1o1bcz	262	45	.	.	.
cord-009318-zt1o1bcz	263	1	Color	color	NN
cord-009318-zt1o1bcz	263	2	intensity	intensity	NN
cord-009318-zt1o1bcz	263	3	indicates	indicate	VBZ
cord-009318-zt1o1bcz	263	4	the	the	DT
cord-009318-zt1o1bcz	263	5	abundance	abundance	NN
cord-009318-zt1o1bcz	263	6	of	of	IN
cord-009318-zt1o1bcz	263	7	paritions	parition	NNS
cord-009318-zt1o1bcz	263	8	at	at	IN
cord-009318-zt1o1bcz	263	9	a	a	DT
cord-009318-zt1o1bcz	263	10	given	give	VBN
cord-009318-zt1o1bcz	263	11	TTP	ttp	NN
cord-009318-zt1o1bcz	263	12	and	and	CC
cord-009318-zt1o1bcz	263	13	temperature	temperature	NN
cord-009318-zt1o1bcz	263	14	.	.	.
cord-009318-zt1o1bcz	264	1	To	to	TO
cord-009318-zt1o1bcz	264	2	further	further	RB
cord-009318-zt1o1bcz	264	3	illustrate	illustrate	VB
cord-009318-zt1o1bcz	264	4	how	how	WRB
cord-009318-zt1o1bcz	264	5	this	this	DT
cord-009318-zt1o1bcz	264	6	approach	approach	NN
cord-009318-zt1o1bcz	264	7	can	can	MD
cord-009318-zt1o1bcz	264	8	be	be	VB
cord-009318-zt1o1bcz	264	9	used	use	VBN
cord-009318-zt1o1bcz	264	10	to	to	TO
cord-009318-zt1o1bcz	264	11	differentiate	differentiate	VB
cord-009318-zt1o1bcz	264	12	specific	specific	JJ
cord-009318-zt1o1bcz	264	13	and	and	CC
cord-009318-zt1o1bcz	264	14	non	non	JJ
cord-009318-zt1o1bcz	264	15	-	-	JJ
cord-009318-zt1o1bcz	264	16	specific	specific	JJ
cord-009318-zt1o1bcz	264	17	amplification	amplification	NN
cord-009318-zt1o1bcz	264	18	,	,	,
cord-009318-zt1o1bcz	264	19	we	-PRON-	PRP
cord-009318-zt1o1bcz	264	20	next	next	RB
cord-009318-zt1o1bcz	264	21	selected	select	VBD
cord-009318-zt1o1bcz	264	22	a	a	DT
cord-009318-zt1o1bcz	264	23	re	re	NN
cord-009318-zt1o1bcz	264	24	-	-	NN
cord-009318-zt1o1bcz	264	25	gion	gion	NN
cord-009318-zt1o1bcz	264	26	where	where	WRB
cord-009318-zt1o1bcz	264	27	both	both	DT
cord-009318-zt1o1bcz	264	28	specific	specific	JJ
cord-009318-zt1o1bcz	264	29	and	and	CC
cord-009318-zt1o1bcz	264	30	non	non	JJ
cord-009318-zt1o1bcz	264	31	-	-	JJ
cord-009318-zt1o1bcz	264	32	specific	specific	JJ
cord-009318-zt1o1bcz	264	33	products	product	NNS
cord-009318-zt1o1bcz	264	34	were	be	VBD
cord-009318-zt1o1bcz	264	35	observed	observe	VBN
cord-009318-zt1o1bcz	264	36	.	.	.
cord-009318-zt1o1bcz	265	1	For	for	IN
cord-009318-zt1o1bcz	265	2	Bst	Bst	NNP
cord-009318-zt1o1bcz	265	3	3.0	3.0	NNP
cord-009318-zt1o1bcz	265	4	,	,	,
cord-009318-zt1o1bcz	265	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	265	6	were	be	VBD
cord-009318-zt1o1bcz	265	7	able	able	JJ
cord-009318-zt1o1bcz	265	8	to	to	TO
cord-009318-zt1o1bcz	265	9	distinguish	distinguish	VB
cord-009318-zt1o1bcz	265	10	the	the	DT
cord-009318-zt1o1bcz	265	11	clustering	clustering	NN
cord-009318-zt1o1bcz	265	12	of	of	IN
cord-009318-zt1o1bcz	265	13	high	high	JJ
cord-009318-zt1o1bcz	265	14	-	-	HYPH
cord-009318-zt1o1bcz	265	15	T	t	NN
cord-009318-zt1o1bcz	265	16	m	m	NN
cord-009318-zt1o1bcz	265	17	non	non	JJ
cord-009318-zt1o1bcz	265	18	-	-	JJ
cord-009318-zt1o1bcz	265	19	specific	specific	JJ
cord-009318-zt1o1bcz	265	20	products	product	NNS
cord-009318-zt1o1bcz	265	21	separately	separately	RB
cord-009318-zt1o1bcz	265	22	from	from	IN
cord-009318-zt1o1bcz	265	23	specific	specific	JJ
cord-009318-zt1o1bcz	265	24	amplification	amplification	NN
cord-009318-zt1o1bcz	265	25	using	use	VBG
cord-009318-zt1o1bcz	265	26	the	the	DT
cord-009318-zt1o1bcz	265	27	threshold	threshold	NN
cord-009318-zt1o1bcz	265	28	of	of	IN
cord-009318-zt1o1bcz	265	29	91	91	CD
cord-009318-zt1o1bcz	265	30	.	.	.
cord-009318-zt1o1bcz	266	1	.75	.75	NFP
cord-009318-zt1o1bcz	267	1	•	•	NNP
cord-009318-zt1o1bcz	268	1	C	C	NNP
cord-009318-zt1o1bcz	268	2	(	(	-LRB-
cord-009318-zt1o1bcz	268	3	Figure	Figure	NNP
cord-009318-zt1o1bcz	268	4	6E	6E	NNP
cord-009318-zt1o1bcz	268	5	)	)	-RRB-
cord-009318-zt1o1bcz	268	6	and	and	CC
cord-009318-zt1o1bcz	269	1	we	-PRON-	PRP
cord-009318-zt1o1bcz	269	2	observed	observe	VBD
cord-009318-zt1o1bcz	269	3	better	well	JJR
cord-009318-zt1o1bcz	269	4	separation	separation	NN
cord-009318-zt1o1bcz	269	5	of	of	IN
cord-009318-zt1o1bcz	269	6	specific	specific	JJ
cord-009318-zt1o1bcz	269	7	and	and	CC
cord-009318-zt1o1bcz	269	8	non	non	JJ
cord-009318-zt1o1bcz	269	9	-	-	JJ
cord-009318-zt1o1bcz	269	10	specific	specific	JJ
cord-009318-zt1o1bcz	269	11	amplification	amplification	NN
cord-009318-zt1o1bcz	269	12	than	than	IN
cord-009318-zt1o1bcz	269	13	with	with	IN
cord-009318-zt1o1bcz	269	14	Bst	Bst	NNP
cord-009318-zt1o1bcz	269	15	2.0	2.0	CD
cord-009318-zt1o1bcz	269	16	(	(	-LRB-
cord-009318-zt1o1bcz	269	17	Figure	figure	NN
cord-009318-zt1o1bcz	269	18	6F	6f	NN
cord-009318-zt1o1bcz	269	19	and	and	CC
cord-009318-zt1o1bcz	269	20	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	269	21	Figure	Figure	NNP
cord-009318-zt1o1bcz	269	22	S8B	s8b	NN
cord-009318-zt1o1bcz	269	23	)	)	-RRB-
cord-009318-zt1o1bcz	269	24	.	.	.
cord-009318-zt1o1bcz	270	1	Both	both	DT
cord-009318-zt1o1bcz	270	2	enzymes	enzyme	NNS
cord-009318-zt1o1bcz	270	3	had	have	VBD
cord-009318-zt1o1bcz	270	4	highly	highly	RB
cord-009318-zt1o1bcz	270	5	variable	variable	JJ
cord-009318-zt1o1bcz	270	6	TTP	ttp	NN
cord-009318-zt1o1bcz	270	7	,	,	,
cord-009318-zt1o1bcz	270	8	which	which	WDT
cord-009318-zt1o1bcz	270	9	we	-PRON-	PRP
cord-009318-zt1o1bcz	270	10	have	have	VBP
cord-009318-zt1o1bcz	270	11	observed	observe	VBN
cord-009318-zt1o1bcz	270	12	previously	previously	RB
cord-009318-zt1o1bcz	270	13	(	(	-LRB-
cord-009318-zt1o1bcz	270	14	23	23	CD
cord-009318-zt1o1bcz	270	15	)	)	-RRB-
cord-009318-zt1o1bcz	270	16	and	and	CC
cord-009318-zt1o1bcz	270	17	attribute	attribute	VBP
cord-009318-zt1o1bcz	270	18	to	to	IN
cord-009318-zt1o1bcz	270	19	stochastic	stochastic	JJ
cord-009318-zt1o1bcz	270	20	initiation	initiation	NN
cord-009318-zt1o1bcz	270	21	of	of	IN
cord-009318-zt1o1bcz	270	22	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	270	23	.	.	.
cord-009318-zt1o1bcz	271	1	Bst	Bst	NNP
cord-009318-zt1o1bcz	271	2	2.0	2.0	CD
cord-009318-zt1o1bcz	271	3	had	have	VBD
cord-009318-zt1o1bcz	271	4	both	both	DT
cord-009318-zt1o1bcz	271	5	earlier	early	JJR
cord-009318-zt1o1bcz	271	6	specific	specific	JJ
cord-009318-zt1o1bcz	271	7	amplification	amplification	NN
cord-009318-zt1o1bcz	271	8	and	and	CC
cord-009318-zt1o1bcz	271	9	later	later	RB
cord-009318-zt1o1bcz	271	10	non	non	JJ
cord-009318-zt1o1bcz	271	11	-	-	JJ
cord-009318-zt1o1bcz	271	12	specific	specific	JJ
cord-009318-zt1o1bcz	271	13	amplification	amplification	NN
cord-009318-zt1o1bcz	271	14	than	than	IN
cord-009318-zt1o1bcz	271	15	Bst	Bst	NNP
cord-009318-zt1o1bcz	271	16	3.0	3.0	CD
cord-009318-zt1o1bcz	271	17	.	.	.
cord-009318-zt1o1bcz	272	1	Bst	Bst	NNP
cord-009318-zt1o1bcz	272	2	2.0	2.0	CD
cord-009318-zt1o1bcz	272	3	reactions	reaction	NNS
cord-009318-zt1o1bcz	272	4	containing	contain	VBG
cord-009318-zt1o1bcz	272	5	template	template	NN
cord-009318-zt1o1bcz	272	6	generally	generally	RB
cord-009318-zt1o1bcz	272	7	started	start	VBD
cord-009318-zt1o1bcz	272	8	at	at	IN
cord-009318-zt1o1bcz	272	9	10	10	CD
cord-009318-zt1o1bcz	272	10	min	min	NN
cord-009318-zt1o1bcz	272	11	,	,	,
cord-009318-zt1o1bcz	272	12	whereas	whereas	IN
cord-009318-zt1o1bcz	272	13	non	non	JJ
cord-009318-zt1o1bcz	272	14	-	-	JJ
cord-009318-zt1o1bcz	272	15	specific	specific	JJ
cord-009318-zt1o1bcz	272	16	amplification	amplification	NN
cord-009318-zt1o1bcz	272	17	began	begin	VBD
cord-009318-zt1o1bcz	272	18	at	at	IN
cord-009318-zt1o1bcz	272	19	∼40	∼40	NNP
cord-009318-zt1o1bcz	272	20	min	min	NNP
cord-009318-zt1o1bcz	272	21	.	.	.
cord-009318-zt1o1bcz	273	1	In	in	IN
cord-009318-zt1o1bcz	273	2	contrast	contrast	NN
cord-009318-zt1o1bcz	273	3	,	,	,
cord-009318-zt1o1bcz	273	4	Bst	Bst	NNP
cord-009318-zt1o1bcz	273	5	3.0	3.0	CD
cord-009318-zt1o1bcz	273	6	reactions	reaction	NNS
cord-009318-zt1o1bcz	273	7	containing	contain	VBG
cord-009318-zt1o1bcz	273	8	template	template	NN
cord-009318-zt1o1bcz	273	9	began	begin	VBD
cord-009318-zt1o1bcz	273	10	at	at	IN
cord-009318-zt1o1bcz	273	11	11.5	11.5	CD
cord-009318-zt1o1bcz	273	12	min	min	NN
cord-009318-zt1o1bcz	273	13	and	and	CC
cord-009318-zt1o1bcz	273	14	non	non	JJ
cord-009318-zt1o1bcz	273	15	-	-	JJ
cord-009318-zt1o1bcz	273	16	specific	specific	JJ
cord-009318-zt1o1bcz	273	17	amplification	amplification	NN
cord-009318-zt1o1bcz	273	18	began	begin	VBD
cord-009318-zt1o1bcz	273	19	at	at	IN
cord-009318-zt1o1bcz	273	20	∼20	∼20	NNS
cord-009318-zt1o1bcz	273	21	min	min	NNP
cord-009318-zt1o1bcz	273	22	.	.	.
cord-009318-zt1o1bcz	274	1	Next	next	RB
cord-009318-zt1o1bcz	274	2	we	-PRON-	PRP
cord-009318-zt1o1bcz	274	3	asked	ask	VBD
cord-009318-zt1o1bcz	274	4	whether	whether	IN
cord-009318-zt1o1bcz	274	5	there	there	EX
cord-009318-zt1o1bcz	274	6	is	be	VBZ
cord-009318-zt1o1bcz	274	7	a	a	DT
cord-009318-zt1o1bcz	274	8	difference	difference	NN
cord-009318-zt1o1bcz	274	9	between	between	IN
cord-009318-zt1o1bcz	274	10	the	the	DT
cord-009318-zt1o1bcz	274	11	maximum	maximum	JJ
cord-009318-zt1o1bcz	274	12	rates	rate	NNS
cord-009318-zt1o1bcz	274	13	of	of	IN
cord-009318-zt1o1bcz	274	14	specific	specific	JJ
cord-009318-zt1o1bcz	274	15	and	and	CC
cord-009318-zt1o1bcz	274	16	non	non	JJ
cord-009318-zt1o1bcz	274	17	-	-	JJ
cord-009318-zt1o1bcz	274	18	specific	specific	JJ
cord-009318-zt1o1bcz	274	19	amplification	amplification	NN
cord-009318-zt1o1bcz	274	20	.	.	.
cord-009318-zt1o1bcz	275	1	Previously	previously	RB
cord-009318-zt1o1bcz	275	2	,	,	,
cord-009318-zt1o1bcz	275	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	275	4	demonstrated	demonstrate	VBD
cord-009318-zt1o1bcz	275	5	that	that	IN
cord-009318-zt1o1bcz	275	6	rate	rate	NN
cord-009318-zt1o1bcz	275	7	could	could	MD
cord-009318-zt1o1bcz	275	8	be	be	VB
cord-009318-zt1o1bcz	275	9	used	use	VBN
cord-009318-zt1o1bcz	275	10	to	to	TO
cord-009318-zt1o1bcz	275	11	correct	correct	VB
cord-009318-zt1o1bcz	275	12	for	for	IN
cord-009318-zt1o1bcz	275	13	some	some	DT
cord-009318-zt1o1bcz	275	14	non	non	JJ
cord-009318-zt1o1bcz	275	15	-	-	JJ
cord-009318-zt1o1bcz	275	16	specific	specific	JJ
cord-009318-zt1o1bcz	275	17	amplification	amplification	NN
cord-009318-zt1o1bcz	275	18	using	use	VBG
cord-009318-zt1o1bcz	275	19	Escherichia	Escherichia	NNP
cord-009318-zt1o1bcz	275	20	coli	coli	NNS
cord-009318-zt1o1bcz	275	21	23S	23S	NNP
cord-009318-zt1o1bcz	275	22	primers	primer	NNS
cord-009318-zt1o1bcz	275	23	(	(	-LRB-
cord-009318-zt1o1bcz	275	24	23	23	CD
cord-009318-zt1o1bcz	275	25	)	)	-RRB-
cord-009318-zt1o1bcz	275	26	,	,	,
cord-009318-zt1o1bcz	275	27	so	so	RB
cord-009318-zt1o1bcz	275	28	we	-PRON-	PRP
cord-009318-zt1o1bcz	275	29	wished	wish	VBD
cord-009318-zt1o1bcz	275	30	to	to	TO
cord-009318-zt1o1bcz	275	31	test	test	VB
cord-009318-zt1o1bcz	275	32	whether	whether	IN
cord-009318-zt1o1bcz	275	33	we	-PRON-	PRP
cord-009318-zt1o1bcz	275	34	could	could	MD
cord-009318-zt1o1bcz	275	35	use	use	VB
cord-009318-zt1o1bcz	275	36	maximum	maximum	JJ
cord-009318-zt1o1bcz	275	37	rate	rate	NN
cord-009318-zt1o1bcz	275	38	as	as	IN
cord-009318-zt1o1bcz	275	39	a	a	DT
cord-009318-zt1o1bcz	275	40	way	way	NN
cord-009318-zt1o1bcz	275	41	to	to	TO
cord-009318-zt1o1bcz	275	42	differentiate	differentiate	VB
cord-009318-zt1o1bcz	275	43	specific	specific	JJ
cord-009318-zt1o1bcz	275	44	and	and	CC
cord-009318-zt1o1bcz	275	45	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	275	46	amplification	amplification	NN
cord-009318-zt1o1bcz	275	47	.	.	.
cord-009318-zt1o1bcz	276	1	Generally	generally	RB
cord-009318-zt1o1bcz	276	2	,	,	,
cord-009318-zt1o1bcz	276	3	specific	specific	JJ
cord-009318-zt1o1bcz	276	4	and	and	CC
cord-009318-zt1o1bcz	276	5	non	non	JJ
cord-009318-zt1o1bcz	276	6	-	-	JJ
cord-009318-zt1o1bcz	276	7	specific	specific	JJ
cord-009318-zt1o1bcz	276	8	amplification	amplification	NN
cord-009318-zt1o1bcz	276	9	reactions	reaction	NNS
cord-009318-zt1o1bcz	276	10	did	do	VBD
cord-009318-zt1o1bcz	276	11	not	not	RB
cord-009318-zt1o1bcz	276	12	have	have	VB
cord-009318-zt1o1bcz	276	13	the	the	DT
cord-009318-zt1o1bcz	276	14	same	same	JJ
cord-009318-zt1o1bcz	276	15	maximum	maximum	JJ
cord-009318-zt1o1bcz	276	16	rate	rate	NN
cord-009318-zt1o1bcz	276	17	.	.	.
cord-009318-zt1o1bcz	277	1	For	for	IN
cord-009318-zt1o1bcz	277	2	Bst	Bst	NNP
cord-009318-zt1o1bcz	277	3	2.0	2.0	CD
cord-009318-zt1o1bcz	277	4	,	,	,
cord-009318-zt1o1bcz	277	5	non	non	JJ
cord-009318-zt1o1bcz	277	6	-	-	JJ
cord-009318-zt1o1bcz	277	7	specific	specific	JJ
cord-009318-zt1o1bcz	277	8	amplification	amplification	NN
cord-009318-zt1o1bcz	277	9	tended	tend	VBD
cord-009318-zt1o1bcz	277	10	to	to	TO
cord-009318-zt1o1bcz	277	11	have	have	VB
cord-009318-zt1o1bcz	277	12	a	a	DT
cord-009318-zt1o1bcz	277	13	slower	slow	JJR
cord-009318-zt1o1bcz	277	14	max	max	NN
cord-009318-zt1o1bcz	277	15	rate	rate	NN
cord-009318-zt1o1bcz	277	16	than	than	IN
cord-009318-zt1o1bcz	277	17	specific	specific	JJ
cord-009318-zt1o1bcz	277	18	amplification	amplification	NN
cord-009318-zt1o1bcz	277	19	,	,	,
cord-009318-zt1o1bcz	277	20	although	although	IN
cord-009318-zt1o1bcz	277	21	there	there	EX
cord-009318-zt1o1bcz	277	22	was	be	VBD
cord-009318-zt1o1bcz	277	23	some	some	DT
cord-009318-zt1o1bcz	277	24	overlap	overlap	NN
cord-009318-zt1o1bcz	277	25	(	(	-LRB-
cord-009318-zt1o1bcz	277	26	Figure	figure	NN
cord-009318-zt1o1bcz	277	27	6	6	CD
cord-009318-zt1o1bcz	277	28	G	g	NN
cord-009318-zt1o1bcz	277	29	)	)	-RRB-
cord-009318-zt1o1bcz	277	30	.	.	.
cord-009318-zt1o1bcz	278	1	At	at	IN
cord-009318-zt1o1bcz	278	2	high	high	JJ
cord-009318-zt1o1bcz	278	3	T	t	NN
cord-009318-zt1o1bcz	278	4	m	m	NN
cord-009318-zt1o1bcz	278	5	,	,	,
cord-009318-zt1o1bcz	278	6	the	the	DT
cord-009318-zt1o1bcz	278	7	clustering	clustering	NN
cord-009318-zt1o1bcz	278	8	of	of	IN
cord-009318-zt1o1bcz	278	9	non	non	JJ
cord-009318-zt1o1bcz	278	10	-	-	JJ
cord-009318-zt1o1bcz	278	11	specific	specific	JJ
cord-009318-zt1o1bcz	278	12	amplification	amplification	NN
cord-009318-zt1o1bcz	278	13	in	in	IN
cord-009318-zt1o1bcz	278	14	both	both	CC
cord-009318-zt1o1bcz	278	15	the	the	DT
cord-009318-zt1o1bcz	278	16	presence	presence	NN
cord-009318-zt1o1bcz	278	17	of	of	IN
cord-009318-zt1o1bcz	278	18	template	template	NN
cord-009318-zt1o1bcz	278	19	and	and	CC
cord-009318-zt1o1bcz	278	20	in	in	IN
cord-009318-zt1o1bcz	278	21	the	the	DT
cord-009318-zt1o1bcz	278	22	NTC	NTC	NNP
cord-009318-zt1o1bcz	278	23	were	be	VBD
cord-009318-zt1o1bcz	278	24	observed	observe	VBN
cord-009318-zt1o1bcz	278	25	at	at	IN
cord-009318-zt1o1bcz	278	26	>	>	XX
cord-009318-zt1o1bcz	278	27	90.5	90.5	CD
cord-009318-zt1o1bcz	278	28	•	•	NN
cord-009318-zt1o1bcz	278	29	C	c	NN
cord-009318-zt1o1bcz	278	30	and	and	CC
cord-009318-zt1o1bcz	278	31	below	below	IN
cord-009318-zt1o1bcz	278	32	∼50	∼50	NNP
cord-009318-zt1o1bcz	278	33	RFU/30	RFU/30	NNP
cord-009318-zt1o1bcz	278	34	s	s	NNP
cord-009318-zt1o1bcz	279	1	(	(	-LRB-
cord-009318-zt1o1bcz	279	2	Figure	figure	NN
cord-009318-zt1o1bcz	279	3	6H	6h	CD
cord-009318-zt1o1bcz	279	4	)	)	-RRB-
cord-009318-zt1o1bcz	279	5	.	.	.
cord-009318-zt1o1bcz	280	1	For	for	IN
cord-009318-zt1o1bcz	280	2	Bst	Bst	NNP
cord-009318-zt1o1bcz	280	3	3.0	3.0	NNP
cord-009318-zt1o1bcz	280	4	,	,	,
cord-009318-zt1o1bcz	280	5	although	although	IN
cord-009318-zt1o1bcz	280	6	there	there	EX
cord-009318-zt1o1bcz	280	7	was	be	VBD
cord-009318-zt1o1bcz	280	8	substantial	substantial	JJ
cord-009318-zt1o1bcz	280	9	overlap	overlap	NN
cord-009318-zt1o1bcz	280	10	,	,	,
cord-009318-zt1o1bcz	280	11	we	-PRON-	PRP
cord-009318-zt1o1bcz	280	12	again	again	RB
cord-009318-zt1o1bcz	280	13	observed	observe	VBD
cord-009318-zt1o1bcz	280	14	that	that	IN
cord-009318-zt1o1bcz	280	15	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	280	16	amplification	amplification	NN
cord-009318-zt1o1bcz	280	17	tended	tend	VBD
cord-009318-zt1o1bcz	280	18	to	to	TO
cord-009318-zt1o1bcz	280	19	have	have	VB
cord-009318-zt1o1bcz	280	20	slower	slow	JJR
cord-009318-zt1o1bcz	280	21	maximum	maximum	JJ
cord-009318-zt1o1bcz	280	22	rate	rate	NN
cord-009318-zt1o1bcz	280	23	than	than	IN
cord-009318-zt1o1bcz	280	24	specific	specific	JJ
cord-009318-zt1o1bcz	280	25	amplification	amplification	NN
cord-009318-zt1o1bcz	280	26	(	(	-LRB-
cord-009318-zt1o1bcz	280	27	Figure	figure	NN
cord-009318-zt1o1bcz	280	28	6I	6i	CD
cord-009318-zt1o1bcz	280	29	)	)	-RRB-
cord-009318-zt1o1bcz	280	30	.	.	.
cord-009318-zt1o1bcz	281	1	Examining	examine	VBG
cord-009318-zt1o1bcz	281	2	the	the	DT
cord-009318-zt1o1bcz	281	3	high	high	JJ
cord-009318-zt1o1bcz	281	4	-	-	HYPH
cord-009318-zt1o1bcz	281	5	T	t	NN
cord-009318-zt1o1bcz	281	6	m	m	NN
cord-009318-zt1o1bcz	281	7	amplification	amplification	NN
cord-009318-zt1o1bcz	281	8	events	event	NNS
cord-009318-zt1o1bcz	281	9	,	,	,
cord-009318-zt1o1bcz	281	10	non	non	JJ
cord-009318-zt1o1bcz	281	11	-	-	JJ
cord-009318-zt1o1bcz	281	12	specific	specific	JJ
cord-009318-zt1o1bcz	281	13	amplification	amplification	NN
cord-009318-zt1o1bcz	281	14	collects	collect	VBZ
cord-009318-zt1o1bcz	281	15	above	above	IN
cord-009318-zt1o1bcz	281	16	92.75	92.75	CD
cord-009318-zt1o1bcz	281	17	•	•	NN
cord-009318-zt1o1bcz	281	18	C	C	NNP
cord-009318-zt1o1bcz	281	19	and	and	CC
cord-009318-zt1o1bcz	281	20	has	have	VBZ
cord-009318-zt1o1bcz	281	21	maximum	maximum	JJ
cord-009318-zt1o1bcz	281	22	rate	rate	NN
cord-009318-zt1o1bcz	281	23	extending	extend	VBG
cord-009318-zt1o1bcz	281	24	out	out	RP
cord-009318-zt1o1bcz	281	25	to	to	TO
cord-009318-zt1o1bcz	281	26	75	75	CD
cord-009318-zt1o1bcz	281	27	RFU/30	RFU/30	NNS
cord-009318-zt1o1bcz	281	28	s	s	NN
cord-009318-zt1o1bcz	281	29	(	(	-LRB-
cord-009318-zt1o1bcz	281	30	Figure	figure	NN
cord-009318-zt1o1bcz	281	31	6J	6j	CD
cord-009318-zt1o1bcz	281	32	)	)	-RRB-
cord-009318-zt1o1bcz	281	33	.	.	.
cord-009318-zt1o1bcz	282	1	For	for	IN
cord-009318-zt1o1bcz	282	2	both	both	DT
cord-009318-zt1o1bcz	282	3	enzymes	enzyme	NNS
cord-009318-zt1o1bcz	282	4	,	,	,
cord-009318-zt1o1bcz	282	5	overlap	overlap	VBP
cord-009318-zt1o1bcz	282	6	between	between	IN
cord-009318-zt1o1bcz	282	7	specific	specific	JJ
cord-009318-zt1o1bcz	282	8	and	and	CC
cord-009318-zt1o1bcz	282	9	non	non	JJ
cord-009318-zt1o1bcz	282	10	-	-	JJ
cord-009318-zt1o1bcz	282	11	specific	specific	JJ
cord-009318-zt1o1bcz	282	12	amplification	amplification	NN
cord-009318-zt1o1bcz	282	13	was	be	VBD
cord-009318-zt1o1bcz	282	14	similar	similar	JJ
cord-009318-zt1o1bcz	282	15	and	and	CC
cord-009318-zt1o1bcz	282	16	specific	specific	JJ
cord-009318-zt1o1bcz	282	17	amplification	amplification	NN
cord-009318-zt1o1bcz	282	18	tended	tend	VBD
cord-009318-zt1o1bcz	282	19	to	to	TO
cord-009318-zt1o1bcz	282	20	be	be	VB
cord-009318-zt1o1bcz	282	21	faster	fast	JJR
cord-009318-zt1o1bcz	282	22	.	.	.
cord-009318-zt1o1bcz	283	1	However	however	RB
cord-009318-zt1o1bcz	283	2	,	,	,
cord-009318-zt1o1bcz	283	3	the	the	DT
cord-009318-zt1o1bcz	283	4	maximum	maximum	JJ
cord-009318-zt1o1bcz	283	5	rate	rate	NN
cord-009318-zt1o1bcz	283	6	of	of	IN
cord-009318-zt1o1bcz	283	7	specific	specific	JJ
cord-009318-zt1o1bcz	283	8	amplification	amplification	NN
cord-009318-zt1o1bcz	283	9	between	between	IN
cord-009318-zt1o1bcz	283	10	the	the	DT
cord-009318-zt1o1bcz	283	11	two	two	CD
cord-009318-zt1o1bcz	283	12	enzymes	enzyme	NNS
cord-009318-zt1o1bcz	283	13	differed	differ	VBD
cord-009318-zt1o1bcz	283	14	;	;	:
cord-009318-zt1o1bcz	283	15	Bst	Bst	NNP
cord-009318-zt1o1bcz	283	16	2.0	2.0	CD
cord-009318-zt1o1bcz	283	17	had	have	VBD
cord-009318-zt1o1bcz	283	18	a	a	DT
cord-009318-zt1o1bcz	283	19	maxium	maxium	JJ
cord-009318-zt1o1bcz	283	20	rate	rate	NN
cord-009318-zt1o1bcz	283	21	of	of	IN
cord-009318-zt1o1bcz	283	22	150	150	CD
cord-009318-zt1o1bcz	283	23	RFU/30	RFU/30	NNS
cord-009318-zt1o1bcz	283	24	s	s	NNP
cord-009318-zt1o1bcz	283	25	,	,	,
cord-009318-zt1o1bcz	283	26	whereas	whereas	IN
cord-009318-zt1o1bcz	283	27	Bst	Bst	NNP
cord-009318-zt1o1bcz	283	28	3.0	3.0	NNP
cord-009318-zt1o1bcz	283	29	did	do	VBD
cord-009318-zt1o1bcz	283	30	not	not	RB
cord-009318-zt1o1bcz	283	31	exceed	exceed	VB
cord-009318-zt1o1bcz	283	32	100	100	CD
cord-009318-zt1o1bcz	283	33	RFU/30	RFU/30	NNS
cord-009318-zt1o1bcz	283	34	s.	s.	NNP
cord-009318-zt1o1bcz	283	35	Bst	Bst	NNP
cord-009318-zt1o1bcz	283	36	2.0	2.0	CD
cord-009318-zt1o1bcz	283	37	performing	perform	VBG
cord-009318-zt1o1bcz	283	38	faster	fast	RBR
cord-009318-zt1o1bcz	283	39	than	than	IN
cord-009318-zt1o1bcz	283	40	Bst	Bst	NNP
cord-009318-zt1o1bcz	283	41	3.0	3.0	CD
cord-009318-zt1o1bcz	284	1	is	be	VBZ
cord-009318-zt1o1bcz	284	2	consistent	consistent	JJ
cord-009318-zt1o1bcz	284	3	with	with	IN
cord-009318-zt1o1bcz	284	4	our	-PRON-	PRP$
cord-009318-zt1o1bcz	284	5	previous	previous	JJ
cord-009318-zt1o1bcz	284	6	observations	observation	NNS
cord-009318-zt1o1bcz	284	7	using	use	VBG
cord-009318-zt1o1bcz	284	8	an	an	DT
cord-009318-zt1o1bcz	284	9	E.	E.	NNP
cord-009318-zt1o1bcz	284	10	Coli	Coli	NNP
cord-009318-zt1o1bcz	284	11	23S	23S	NNP
cord-009318-zt1o1bcz	284	12	primer	primer	NN
cord-009318-zt1o1bcz	284	13	set	set	NN
cord-009318-zt1o1bcz	284	14	(	(	-LRB-
cord-009318-zt1o1bcz	284	15	23	23	CD
cord-009318-zt1o1bcz	284	16	)	)	-RRB-
cord-009318-zt1o1bcz	284	17	.	.	.
cord-009318-zt1o1bcz	285	1	Additionally	additionally	RB
cord-009318-zt1o1bcz	285	2	,	,	,
cord-009318-zt1o1bcz	285	3	the	the	DT
cord-009318-zt1o1bcz	285	4	maximum	maximum	JJ
cord-009318-zt1o1bcz	285	5	rate	rate	NN
cord-009318-zt1o1bcz	285	6	of	of	IN
cord-009318-zt1o1bcz	285	7	non	non	JJ
cord-009318-zt1o1bcz	285	8	-	-	JJ
cord-009318-zt1o1bcz	285	9	specific	specific	JJ
cord-009318-zt1o1bcz	285	10	amplification	amplification	NN
cord-009318-zt1o1bcz	285	11	in	in	IN
cord-009318-zt1o1bcz	285	12	Bst	Bst	NNP
cord-009318-zt1o1bcz	285	13	2.0	2.0	NNP
cord-009318-zt1o1bcz	285	14	tended	tend	VBD
cord-009318-zt1o1bcz	285	15	to	to	TO
cord-009318-zt1o1bcz	285	16	be	be	VB
cord-009318-zt1o1bcz	285	17	lower	low	JJR
cord-009318-zt1o1bcz	285	18	than	than	IN
cord-009318-zt1o1bcz	285	19	non	non	JJ
cord-009318-zt1o1bcz	285	20	-	-	JJ
cord-009318-zt1o1bcz	285	21	specific	specific	JJ
cord-009318-zt1o1bcz	285	22	amplification	amplification	NN
cord-009318-zt1o1bcz	285	23	in	in	IN
cord-009318-zt1o1bcz	285	24	Bst	Bst	NNP
cord-009318-zt1o1bcz	285	25	3.0	3.0	NNP
cord-009318-zt1o1bcz	285	26	(	(	-LRB-
cord-009318-zt1o1bcz	285	27	50	50	CD
cord-009318-zt1o1bcz	285	28	and	and	CC
cord-009318-zt1o1bcz	285	29	75	75	CD
cord-009318-zt1o1bcz	285	30	RFU/30	RFU/30	NNS
cord-009318-zt1o1bcz	285	31	s	s	NNS
cord-009318-zt1o1bcz	285	32	,	,	,
cord-009318-zt1o1bcz	285	33	respectively	respectively	RB
cord-009318-zt1o1bcz	285	34	)	)	-RRB-
cord-009318-zt1o1bcz	285	35	.	.	.
cord-009318-zt1o1bcz	286	1	Consequently	consequently	RB
cord-009318-zt1o1bcz	286	2	,	,	,
cord-009318-zt1o1bcz	286	3	the	the	DT
cord-009318-zt1o1bcz	286	4	extent	extent	NN
cord-009318-zt1o1bcz	286	5	of	of	IN
cord-009318-zt1o1bcz	286	6	overlap	overlap	NN
cord-009318-zt1o1bcz	286	7	of	of	IN
cord-009318-zt1o1bcz	286	8	specific	specific	JJ
cord-009318-zt1o1bcz	286	9	and	and	CC
cord-009318-zt1o1bcz	286	10	non	non	JJ
cord-009318-zt1o1bcz	286	11	-	-	JJ
cord-009318-zt1o1bcz	286	12	specific	specific	JJ
cord-009318-zt1o1bcz	286	13	amplificaiton	amplificaiton	NN
cord-009318-zt1o1bcz	286	14	was	be	VBD
cord-009318-zt1o1bcz	286	15	greater	great	JJR
cord-009318-zt1o1bcz	286	16	for	for	IN
cord-009318-zt1o1bcz	286	17	Bst	Bst	NNP
cord-009318-zt1o1bcz	286	18	3.0	3.0	NNP
cord-009318-zt1o1bcz	286	19	than	than	IN
cord-009318-zt1o1bcz	286	20	Bst	Bst	NNP
cord-009318-zt1o1bcz	286	21	2.0	2.0	CD
cord-009318-zt1o1bcz	286	22	.	.	.
cord-009318-zt1o1bcz	287	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	287	2	observed	observe	VBD
cord-009318-zt1o1bcz	287	3	an	an	DT
cord-009318-zt1o1bcz	287	4	unexpected	unexpected	JJ
cord-009318-zt1o1bcz	287	5	relationship	relationship	NN
cord-009318-zt1o1bcz	287	6	between	between	IN
cord-009318-zt1o1bcz	287	7	the	the	DT
cord-009318-zt1o1bcz	287	8	final	final	JJ
cord-009318-zt1o1bcz	287	9	intensity	intensity	NN
cord-009318-zt1o1bcz	287	10	of	of	IN
cord-009318-zt1o1bcz	287	11	each	each	DT
cord-009318-zt1o1bcz	287	12	partition	partition	NN
cord-009318-zt1o1bcz	287	13	and	and	CC
cord-009318-zt1o1bcz	287	14	the	the	DT
cord-009318-zt1o1bcz	287	15	maximum	maximum	JJ
cord-009318-zt1o1bcz	287	16	rate	rate	NN
cord-009318-zt1o1bcz	287	17	of	of	IN
cord-009318-zt1o1bcz	287	18	that	that	DT
cord-009318-zt1o1bcz	287	19	partition	partition	NN
cord-009318-zt1o1bcz	287	20	.	.	.
cord-009318-zt1o1bcz	288	1	After	after	IN
cord-009318-zt1o1bcz	288	2	90	90	CD
cord-009318-zt1o1bcz	288	3	min	min	NN
cord-009318-zt1o1bcz	288	4	of	of	IN
cord-009318-zt1o1bcz	288	5	amplification	amplification	NN
cord-009318-zt1o1bcz	288	6	,	,	,
cord-009318-zt1o1bcz	288	7	a	a	DT
cord-009318-zt1o1bcz	288	8	partition	partition	NN
cord-009318-zt1o1bcz	288	9	should	should	MD
cord-009318-zt1o1bcz	288	10	theoretically	theoretically	RB
cord-009318-zt1o1bcz	288	11	reach	reach	VB
cord-009318-zt1o1bcz	288	12	a	a	DT
cord-009318-zt1o1bcz	288	13	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	288	14	maximum	maximum	NN
cord-009318-zt1o1bcz	288	15	whereby	whereby	WRB
cord-009318-zt1o1bcz	288	16	all	all	DT
cord-009318-zt1o1bcz	288	17	reagents	reagent	NNS
cord-009318-zt1o1bcz	288	18	are	be	VBP
cord-009318-zt1o1bcz	288	19	consumed	consume	VBN
cord-009318-zt1o1bcz	288	20	,	,	,
cord-009318-zt1o1bcz	288	21	amplification	amplification	NN
cord-009318-zt1o1bcz	288	22	plateaus	plateaus	NN
cord-009318-zt1o1bcz	288	23	and	and	CC
cord-009318-zt1o1bcz	288	24	thus	thus	RB
cord-009318-zt1o1bcz	288	25	the	the	DT
cord-009318-zt1o1bcz	288	26	final	final	JJ
cord-009318-zt1o1bcz	288	27	intensity	intensity	NN
cord-009318-zt1o1bcz	288	28	would	would	MD
cord-009318-zt1o1bcz	288	29	be	be	VB
cord-009318-zt1o1bcz	288	30	independent	independent	JJ
cord-009318-zt1o1bcz	288	31	of	of	IN
cord-009318-zt1o1bcz	288	32	the	the	DT
cord-009318-zt1o1bcz	288	33	maximum	maximum	JJ
cord-009318-zt1o1bcz	288	34	rate	rate	NN
cord-009318-zt1o1bcz	288	35	of	of	IN
cord-009318-zt1o1bcz	288	36	amplification	amplification	NN
cord-009318-zt1o1bcz	288	37	.	.	.
cord-009318-zt1o1bcz	289	1	However	however	RB
cord-009318-zt1o1bcz	289	2	,	,	,
cord-009318-zt1o1bcz	289	3	surprisingly	surprisingly	RB
cord-009318-zt1o1bcz	289	4	,	,	,
cord-009318-zt1o1bcz	289	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	289	6	observed	observe	VBD
cord-009318-zt1o1bcz	289	7	a	a	DT
cord-009318-zt1o1bcz	289	8	general	general	JJ
cord-009318-zt1o1bcz	289	9	scaling	scaling	NN
cord-009318-zt1o1bcz	289	10	between	between	IN
cord-009318-zt1o1bcz	289	11	the	the	DT
cord-009318-zt1o1bcz	289	12	maximum	maximum	JJ
cord-009318-zt1o1bcz	289	13	rate	rate	NN
cord-009318-zt1o1bcz	289	14	and	and	CC
cord-009318-zt1o1bcz	289	15	the	the	DT
cord-009318-zt1o1bcz	289	16	final	final	JJ
cord-009318-zt1o1bcz	289	17	intensity	intensity	NN
cord-009318-zt1o1bcz	289	18	of	of	IN
cord-009318-zt1o1bcz	289	19	the	the	DT
cord-009318-zt1o1bcz	289	20	partition	partition	NN
cord-009318-zt1o1bcz	289	21	.	.	.
cord-009318-zt1o1bcz	290	1	For	for	IN
cord-009318-zt1o1bcz	290	2	Bst	Bst	NNP
cord-009318-zt1o1bcz	290	3	2.0	2.0	CD
cord-009318-zt1o1bcz	290	4	,	,	,
cord-009318-zt1o1bcz	290	5	all	all	DT
cord-009318-zt1o1bcz	290	6	amplification	amplification	NN
cord-009318-zt1o1bcz	290	7	in	in	IN
cord-009318-zt1o1bcz	290	8	the	the	DT
cord-009318-zt1o1bcz	290	9	NTC	NTC	NNP
cord-009318-zt1o1bcz	290	10	has	have	VBZ
cord-009318-zt1o1bcz	290	11	final	final	JJ
cord-009318-zt1o1bcz	290	12	intensity	intensity	NN
cord-009318-zt1o1bcz	291	1	<	<	XX
cord-009318-zt1o1bcz	291	2	1017	1017	CD
cord-009318-zt1o1bcz	292	1	RFU	rfu	NN
cord-009318-zt1o1bcz	292	2	and	and	CC
cord-009318-zt1o1bcz	292	3	maximum	maximum	JJ
cord-009318-zt1o1bcz	292	4	rate	rate	NN
cord-009318-zt1o1bcz	292	5	<	<	XX
cord-009318-zt1o1bcz	292	6	53.4	53.4	CD
cord-009318-zt1o1bcz	292	7	RFU/30	RFU/30	NNS
cord-009318-zt1o1bcz	292	8	s.	s.	NNP
cord-009318-zt1o1bcz	293	1	In	in	IN
cord-009318-zt1o1bcz	293	2	the	the	DT
cord-009318-zt1o1bcz	293	3	presence	presence	NN
cord-009318-zt1o1bcz	293	4	of	of	IN
cord-009318-zt1o1bcz	293	5	template	template	NN
cord-009318-zt1o1bcz	293	6	,	,	,
cord-009318-zt1o1bcz	293	7	79.7	79.7	CD
cord-009318-zt1o1bcz	293	8	%	%	NN
cord-009318-zt1o1bcz	293	9	of	of	IN
cord-009318-zt1o1bcz	293	10	non	non	JJ
cord-009318-zt1o1bcz	293	11	-	-	JJ
cord-009318-zt1o1bcz	293	12	specific	specific	JJ
cord-009318-zt1o1bcz	293	13	amplification	amplification	NN
cord-009318-zt1o1bcz	293	14	and	and	CC
cord-009318-zt1o1bcz	293	15	52.3	52.3	CD
cord-009318-zt1o1bcz	293	16	%	%	NN
cord-009318-zt1o1bcz	293	17	of	of	IN
cord-009318-zt1o1bcz	293	18	specific	specific	JJ
cord-009318-zt1o1bcz	293	19	amplification	amplification	NN
cord-009318-zt1o1bcz	293	20	had	have	VBD
cord-009318-zt1o1bcz	293	21	final	final	JJ
cord-009318-zt1o1bcz	293	22	intensity	intensity	NN
cord-009318-zt1o1bcz	293	23	and	and	CC
cord-009318-zt1o1bcz	293	24	maximum	maximum	JJ
cord-009318-zt1o1bcz	293	25	rate	rate	NN
cord-009318-zt1o1bcz	293	26	less	less	JJR
cord-009318-zt1o1bcz	293	27	than	than	IN
cord-009318-zt1o1bcz	293	28	these	these	DT
cord-009318-zt1o1bcz	293	29	thresholds	threshold	NNS
cord-009318-zt1o1bcz	293	30	.	.	.
cord-009318-zt1o1bcz	294	1	For	for	IN
cord-009318-zt1o1bcz	294	2	45	45	CD
cord-009318-zt1o1bcz	294	3	.6	.6	CD
cord-009318-zt1o1bcz	294	4	%	%	NN
cord-009318-zt1o1bcz	294	5	of	of	IN
cord-009318-zt1o1bcz	294	6	specific	specific	JJ
cord-009318-zt1o1bcz	294	7	amplification	amplification	NN
cord-009318-zt1o1bcz	294	8	fell	fall	VBD
cord-009318-zt1o1bcz	294	9	within	within	IN
cord-009318-zt1o1bcz	294	10	these	these	DT
cord-009318-zt1o1bcz	294	11	thresholds	threshold	NNS
cord-009318-zt1o1bcz	294	12	using	use	VBG
cord-009318-zt1o1bcz	294	13	Bst	Bst	NNP
cord-009318-zt1o1bcz	294	14	3.0	3.0	NNP
cord-009318-zt1o1bcz	294	15	.	.	.
cord-009318-zt1o1bcz	295	1	Thus	thus	RB
cord-009318-zt1o1bcz	295	2	,	,	,
cord-009318-zt1o1bcz	295	3	false	false	JJ
cord-009318-zt1o1bcz	295	4	positives	positive	NNS
cord-009318-zt1o1bcz	295	5	were	be	VBD
cord-009318-zt1o1bcz	295	6	generally	generally	RB
cord-009318-zt1o1bcz	295	7	dimmer	dimmer	JJ
cord-009318-zt1o1bcz	295	8	and	and	CC
cord-009318-zt1o1bcz	295	9	had	have	VBD
cord-009318-zt1o1bcz	295	10	slower	slow	JJR
cord-009318-zt1o1bcz	295	11	maximum	maximum	JJ
cord-009318-zt1o1bcz	295	12	rates	rate	NNS
cord-009318-zt1o1bcz	295	13	than	than	IN
cord-009318-zt1o1bcz	295	14	most	most	JJS
cord-009318-zt1o1bcz	295	15	true	true	JJ
cord-009318-zt1o1bcz	295	16	-	-	HYPH
cord-009318-zt1o1bcz	295	17	positive	positive	JJ
cord-009318-zt1o1bcz	295	18	events	event	NNS
cord-009318-zt1o1bcz	295	19	.	.	.
cord-009318-zt1o1bcz	296	1	When	when	WRB
cord-009318-zt1o1bcz	296	2	examining	examine	VBG
cord-009318-zt1o1bcz	296	3	the	the	DT
cord-009318-zt1o1bcz	296	4	brightest	bright	JJS
cord-009318-zt1o1bcz	296	5	partitions	partition	NNS
cord-009318-zt1o1bcz	296	6	,	,	,
cord-009318-zt1o1bcz	296	7	Bst	Bst	NNP
cord-009318-zt1o1bcz	296	8	2.0	2.0	CD
cord-009318-zt1o1bcz	296	9	(	(	-LRB-
cord-009318-zt1o1bcz	296	10	Figure	figure	NN
cord-009318-zt1o1bcz	296	11	6	6	CD
cord-009318-zt1o1bcz	296	12	K	K	NNP
cord-009318-zt1o1bcz	296	13	)	)	-RRB-
cord-009318-zt1o1bcz	296	14	and	and	CC
cord-009318-zt1o1bcz	296	15	Bst	Bst	NNP
cord-009318-zt1o1bcz	296	16	3.0	3.0	NNP
cord-009318-zt1o1bcz	296	17	(	(	-LRB-
cord-009318-zt1o1bcz	296	18	Figure	figure	NN
cord-009318-zt1o1bcz	296	19	6L	6l	CD
cord-009318-zt1o1bcz	296	20	)	)	-RRB-
cord-009318-zt1o1bcz	296	21	exhibit	exhibit	VBP
cord-009318-zt1o1bcz	296	22	a	a	DT
cord-009318-zt1o1bcz	296	23	similar	similar	JJ
cord-009318-zt1o1bcz	296	24	maximal	maximal	JJ
cord-009318-zt1o1bcz	296	25	final	final	JJ
cord-009318-zt1o1bcz	296	26	intensity	intensity	NN
cord-009318-zt1o1bcz	296	27	near	near	IN
cord-009318-zt1o1bcz	296	28	3000	3000	CD
cord-009318-zt1o1bcz	296	29	RFU	rfu	NN
cord-009318-zt1o1bcz	296	30	.	.	.
cord-009318-zt1o1bcz	297	1	These	these	DT
cord-009318-zt1o1bcz	297	2	maxima	maximum	NNS
cord-009318-zt1o1bcz	297	3	are	be	VBP
cord-009318-zt1o1bcz	297	4	also	also	RB
cord-009318-zt1o1bcz	297	5	surprising	surprising	JJ
cord-009318-zt1o1bcz	297	6	,	,	,
cord-009318-zt1o1bcz	297	7	considering	consider	VBG
cord-009318-zt1o1bcz	297	8	our	-PRON-	PRP$
cord-009318-zt1o1bcz	297	9	12-bit	12-bit	CD
cord-009318-zt1o1bcz	297	10	camera	camera	NN
cord-009318-zt1o1bcz	297	11	is	be	VBZ
cord-009318-zt1o1bcz	297	12	capable	capable	JJ
cord-009318-zt1o1bcz	297	13	of	of	IN
cord-009318-zt1o1bcz	297	14	imaging	image	VBG
cord-009318-zt1o1bcz	297	15	up	up	RP
cord-009318-zt1o1bcz	297	16	to	to	IN
cord-009318-zt1o1bcz	297	17	4096	4096	CD
cord-009318-zt1o1bcz	297	18	RFU	rfu	NN
cord-009318-zt1o1bcz	297	19	(	(	-LRB-
cord-009318-zt1o1bcz	297	20	the	the	DT
cord-009318-zt1o1bcz	297	21	detector	detector	NN
cord-009318-zt1o1bcz	297	22	was	be	VBD
cord-009318-zt1o1bcz	297	23	not	not	RB
cord-009318-zt1o1bcz	297	24	at	at	IN
cord-009318-zt1o1bcz	297	25	saturation	saturation	NN
cord-009318-zt1o1bcz	297	26	)	)	-RRB-
cord-009318-zt1o1bcz	297	27	.	.	.
cord-009318-zt1o1bcz	298	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	298	2	suspect	suspect	VBP
cord-009318-zt1o1bcz	298	3	that	that	IN
cord-009318-zt1o1bcz	298	4	this	this	DT
cord-009318-zt1o1bcz	298	5	maxima	maxima	NN
cord-009318-zt1o1bcz	298	6	corresponds	correspond	VBZ
cord-009318-zt1o1bcz	298	7	to	to	IN
cord-009318-zt1o1bcz	298	8	consumption	consumption	NN
cord-009318-zt1o1bcz	298	9	of	of	IN
cord-009318-zt1o1bcz	298	10	one	one	CD
cord-009318-zt1o1bcz	298	11	of	of	IN
cord-009318-zt1o1bcz	298	12	the	the	DT
cord-009318-zt1o1bcz	298	13	reagents	reagent	NNS
cord-009318-zt1o1bcz	298	14	;	;	:
cord-009318-zt1o1bcz	298	15	while	while	IN
cord-009318-zt1o1bcz	298	16	scaling	scale	VBG
cord-009318-zt1o1bcz	298	17	between	between	IN
cord-009318-zt1o1bcz	298	18	maximum	maximum	JJ
cord-009318-zt1o1bcz	298	19	rate	rate	NN
cord-009318-zt1o1bcz	298	20	and	and	CC
cord-009318-zt1o1bcz	298	21	final	final	JJ
cord-009318-zt1o1bcz	298	22	intensity	intensity	NN
cord-009318-zt1o1bcz	298	23	occurs	occur	VBZ
cord-009318-zt1o1bcz	298	24	when	when	WRB
cord-009318-zt1o1bcz	298	25	stochastically	stochastically	RB
cord-009318-zt1o1bcz	298	26	initiated	initiate	VBN
cord-009318-zt1o1bcz	298	27	reactions	reaction	NNS
cord-009318-zt1o1bcz	298	28	have	have	VBP
cord-009318-zt1o1bcz	298	29	not	not	RB
cord-009318-zt1o1bcz	298	30	completely	completely	RB
cord-009318-zt1o1bcz	298	31	amplified	amplify	VBN
cord-009318-zt1o1bcz	298	32	,	,	,
cord-009318-zt1o1bcz	298	33	resulting	result	VBG
cord-009318-zt1o1bcz	298	34	in	in	IN
cord-009318-zt1o1bcz	298	35	partitions	partition	NNS
cord-009318-zt1o1bcz	298	36	dimmer	dimmer	NN
cord-009318-zt1o1bcz	298	37	than	than	IN
cord-009318-zt1o1bcz	298	38	the	the	DT
cord-009318-zt1o1bcz	298	39	maxima	maxima	NN
cord-009318-zt1o1bcz	298	40	and	and	CC
cord-009318-zt1o1bcz	298	41	proportional	proportional	JJ
cord-009318-zt1o1bcz	298	42	to	to	IN
cord-009318-zt1o1bcz	298	43	their	-PRON-	PRP$
cord-009318-zt1o1bcz	298	44	rate	rate	NN
cord-009318-zt1o1bcz	298	45	of	of	IN
cord-009318-zt1o1bcz	298	46	amplification	amplification	NN
cord-009318-zt1o1bcz	298	47	.	.	.
cord-009318-zt1o1bcz	299	1	During	during	IN
cord-009318-zt1o1bcz	299	2	these	these	DT
cord-009318-zt1o1bcz	299	3	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	299	4	experiments	experiment	NNS
cord-009318-zt1o1bcz	299	5	,	,	,
cord-009318-zt1o1bcz	299	6	we	-PRON-	PRP
cord-009318-zt1o1bcz	299	7	also	also	RB
cord-009318-zt1o1bcz	299	8	observed	observe	VBD
cord-009318-zt1o1bcz	299	9	a	a	DT
cord-009318-zt1o1bcz	299	10	relationship	relationship	NN
cord-009318-zt1o1bcz	299	11	between	between	IN
cord-009318-zt1o1bcz	299	12	maximum	maximum	JJ
cord-009318-zt1o1bcz	299	13	rate	rate	NN
cord-009318-zt1o1bcz	299	14	and	and	CC
cord-009318-zt1o1bcz	299	15	TTP	ttp	NN
cord-009318-zt1o1bcz	299	16	.	.	.
cord-009318-zt1o1bcz	300	1	In	in	IN
cord-009318-zt1o1bcz	300	2	bulk	bulk	JJ
cord-009318-zt1o1bcz	300	3	reactions	reaction	NNS
cord-009318-zt1o1bcz	300	4	,	,	,
cord-009318-zt1o1bcz	300	5	the	the	DT
cord-009318-zt1o1bcz	300	6	first	first	JJ
cord-009318-zt1o1bcz	300	7	and	and	CC
cord-009318-zt1o1bcz	300	8	fastest	fast	JJS
cord-009318-zt1o1bcz	300	9	amplification	amplification	NN
cord-009318-zt1o1bcz	300	10	event	event	NN
cord-009318-zt1o1bcz	300	11	determines	determine	VBZ
cord-009318-zt1o1bcz	300	12	the	the	DT
cord-009318-zt1o1bcz	300	13	reaction	reaction	NN
cord-009318-zt1o1bcz	300	14	outcome	outcome	NN
cord-009318-zt1o1bcz	300	15	by	by	IN
cord-009318-zt1o1bcz	300	16	consuming	consume	VBG
cord-009318-zt1o1bcz	300	17	all	all	DT
cord-009318-zt1o1bcz	300	18	of	of	IN
cord-009318-zt1o1bcz	300	19	the	the	DT
cord-009318-zt1o1bcz	300	20	reagents	reagent	NNS
cord-009318-zt1o1bcz	300	21	.	.	.
cord-009318-zt1o1bcz	301	1	Thus	thus	RB
cord-009318-zt1o1bcz	301	2	,	,	,
cord-009318-zt1o1bcz	301	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	301	4	hypothesized	hypothesize	VBD
cord-009318-zt1o1bcz	301	5	that	that	IN
cord-009318-zt1o1bcz	301	6	reaction	reaction	NN
cord-009318-zt1o1bcz	301	7	conditions	condition	NNS
cord-009318-zt1o1bcz	301	8	that	that	WDT
cord-009318-zt1o1bcz	301	9	promote	promote	VBP
cord-009318-zt1o1bcz	301	10	fast	fast	JJ
cord-009318-zt1o1bcz	301	11	and	and	CC
cord-009318-zt1o1bcz	301	12	early	early	JJ
cord-009318-zt1o1bcz	301	13	amplification	amplification	NN
cord-009318-zt1o1bcz	301	14	in	in	IN
cord-009318-zt1o1bcz	301	15	the	the	DT
cord-009318-zt1o1bcz	301	16	NTC	NTC	NNP
cord-009318-zt1o1bcz	301	17	would	would	MD
cord-009318-zt1o1bcz	301	18	lead	lead	VB
cord-009318-zt1o1bcz	301	19	to	to	IN
cord-009318-zt1o1bcz	301	20	a	a	DT
cord-009318-zt1o1bcz	301	21	high	high	JJ
cord-009318-zt1o1bcz	301	22	false	false	JJ
cord-009318-zt1o1bcz	301	23	-	-	HYPH
cord-009318-zt1o1bcz	301	24	positive	positive	JJ
cord-009318-zt1o1bcz	301	25	rate	rate	NN
cord-009318-zt1o1bcz	301	26	in	in	IN
cord-009318-zt1o1bcz	301	27	bulk	bulk	NN
cord-009318-zt1o1bcz	301	28	and	and	CC
cord-009318-zt1o1bcz	301	29	thus	thus	RB
cord-009318-zt1o1bcz	301	30	misidentification	misidentification	NN
cord-009318-zt1o1bcz	301	31	of	of	IN
cord-009318-zt1o1bcz	301	32	amplification	amplification	NN
cord-009318-zt1o1bcz	301	33	.	.	.
cord-009318-zt1o1bcz	302	1	In	in	IN
cord-009318-zt1o1bcz	302	2	both	both	DT
cord-009318-zt1o1bcz	302	3	Bst	Bst	NNP
cord-009318-zt1o1bcz	302	4	2.0	2.0	CD
cord-009318-zt1o1bcz	302	5	(	(	-LRB-
cord-009318-zt1o1bcz	302	6	Figure	figure	NN
cord-009318-zt1o1bcz	302	7	6	6	CD
cord-009318-zt1o1bcz	302	8	M	M	NNP
cord-009318-zt1o1bcz	302	9	)	)	-RRB-
cord-009318-zt1o1bcz	302	10	and	and	CC
cord-009318-zt1o1bcz	302	11	Bst	Bst	NNP
cord-009318-zt1o1bcz	302	12	3.0	3.0	NNP
cord-009318-zt1o1bcz	302	13	(	(	-LRB-
cord-009318-zt1o1bcz	302	14	Figure	figure	NN
cord-009318-zt1o1bcz	302	15	 	 	_SP
cord-009318-zt1o1bcz	302	16	6N	6n	NN
cord-009318-zt1o1bcz	302	17	)	)	-RRB-
cord-009318-zt1o1bcz	302	18	we	-PRON-	PRP
cord-009318-zt1o1bcz	302	19	observed	observe	VBD
cord-009318-zt1o1bcz	302	20	a	a	DT
cord-009318-zt1o1bcz	302	21	general	general	JJ
cord-009318-zt1o1bcz	302	22	trend	trend	NN
cord-009318-zt1o1bcz	302	23	of	of	IN
cord-009318-zt1o1bcz	302	24	fast	fast	JJ
cord-009318-zt1o1bcz	302	25	amplification	amplification	NN
cord-009318-zt1o1bcz	302	26	events	event	NNS
cord-009318-zt1o1bcz	302	27	occurring	occur	VBG
cord-009318-zt1o1bcz	302	28	earlier	early	RBR
cord-009318-zt1o1bcz	302	29	,	,	,
cord-009318-zt1o1bcz	302	30	and	and	CC
cord-009318-zt1o1bcz	302	31	slow	slow	JJ
cord-009318-zt1o1bcz	302	32	events	event	NNS
cord-009318-zt1o1bcz	302	33	occurring	occur	VBG
cord-009318-zt1o1bcz	302	34	later	later	RB
cord-009318-zt1o1bcz	302	35	.	.	.
cord-009318-zt1o1bcz	303	1	In	in	IN
cord-009318-zt1o1bcz	303	2	Bst	Bst	NNP
cord-009318-zt1o1bcz	303	3	2.0	2.0	NNP
cord-009318-zt1o1bcz	303	4	,	,	,
cord-009318-zt1o1bcz	303	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	303	6	observed	observe	VBD
cord-009318-zt1o1bcz	303	7	greater	great	JJR
cord-009318-zt1o1bcz	303	8	heterogeneity	heterogeneity	NN
cord-009318-zt1o1bcz	303	9	in	in	IN
cord-009318-zt1o1bcz	303	10	TTP	ttp	NN
cord-009318-zt1o1bcz	303	11	and	and	CC
cord-009318-zt1o1bcz	303	12	rate	rate	NN
cord-009318-zt1o1bcz	303	13	than	than	IN
cord-009318-zt1o1bcz	303	14	in	in	IN
cord-009318-zt1o1bcz	303	15	Bst	Bst	NNP
cord-009318-zt1o1bcz	303	16	3.0	3.0	NNP
cord-009318-zt1o1bcz	303	17	.	.	.
cord-009318-zt1o1bcz	304	1	Furthermore	furthermore	RB
cord-009318-zt1o1bcz	304	2	,	,	,
cord-009318-zt1o1bcz	304	3	non	non	JJ
cord-009318-zt1o1bcz	304	4	-	-	JJ
cord-009318-zt1o1bcz	304	5	specific	specific	JJ
cord-009318-zt1o1bcz	304	6	amplicons	amplicon	NNS
cord-009318-zt1o1bcz	304	7	in	in	IN
cord-009318-zt1o1bcz	304	8	the	the	DT
cord-009318-zt1o1bcz	304	9	NTC	NTC	NNP
cord-009318-zt1o1bcz	304	10	tended	tend	VBD
cord-009318-zt1o1bcz	304	11	to	to	TO
cord-009318-zt1o1bcz	304	12	produce	produce	VB
cord-009318-zt1o1bcz	304	13	slower	slow	JJR
cord-009318-zt1o1bcz	304	14	and	and	CC
cord-009318-zt1o1bcz	304	15	later	later	JJ
cord-009318-zt1o1bcz	304	16	amplification	amplification	NN
cord-009318-zt1o1bcz	304	17	events	event	NNS
cord-009318-zt1o1bcz	304	18	.	.	.
cord-009318-zt1o1bcz	305	1	Occasional	occasional	JJ
cord-009318-zt1o1bcz	305	2	outliers	outlier	NNS
cord-009318-zt1o1bcz	305	3	occurred	occur	VBD
cord-009318-zt1o1bcz	305	4	at	at	IN
cord-009318-zt1o1bcz	305	5	both	both	CC
cord-009318-zt1o1bcz	305	6	fast	fast	JJ
cord-009318-zt1o1bcz	305	7	and	and	CC
cord-009318-zt1o1bcz	305	8	early	early	JJ
cord-009318-zt1o1bcz	305	9	times	time	NNS
cord-009318-zt1o1bcz	305	10	.	.	.
cord-009318-zt1o1bcz	306	1	Next	next	RB
cord-009318-zt1o1bcz	306	2	,	,	,
cord-009318-zt1o1bcz	306	3	to	to	TO
cord-009318-zt1o1bcz	306	4	explicitly	explicitly	RB
cord-009318-zt1o1bcz	306	5	test	test	VB
cord-009318-zt1o1bcz	306	6	whether	whether	IN
cord-009318-zt1o1bcz	306	7	fast	fast	JJ
cord-009318-zt1o1bcz	306	8	and	and	CC
cord-009318-zt1o1bcz	306	9	early	early	JJ
cord-009318-zt1o1bcz	306	10	events	event	NNS
cord-009318-zt1o1bcz	306	11	correspond	correspond	VBP
cord-009318-zt1o1bcz	306	12	to	to	IN
cord-009318-zt1o1bcz	306	13	specific	specific	JJ
cord-009318-zt1o1bcz	306	14	amplification	amplification	NN
cord-009318-zt1o1bcz	306	15	,	,	,
cord-009318-zt1o1bcz	306	16	we	-PRON-	PRP
cord-009318-zt1o1bcz	306	17	analyzed	analyze	VBD
cord-009318-zt1o1bcz	306	18	the	the	DT
cord-009318-zt1o1bcz	306	19	relationship	relationship	NN
cord-009318-zt1o1bcz	306	20	between	between	IN
cord-009318-zt1o1bcz	306	21	a	a	DT
cord-009318-zt1o1bcz	306	22	partition	partition	NN
cord-009318-zt1o1bcz	306	23	's	's	POS
cord-009318-zt1o1bcz	306	24	TTP	ttp	NN
cord-009318-zt1o1bcz	306	25	,	,	,
cord-009318-zt1o1bcz	306	26	its	-PRON-	PRP$
cord-009318-zt1o1bcz	306	27	maximum	maximum	JJ
cord-009318-zt1o1bcz	306	28	rate	rate	NN
cord-009318-zt1o1bcz	306	29	,	,	,
cord-009318-zt1o1bcz	306	30	and	and	CC
cord-009318-zt1o1bcz	306	31	T	T	NNP
cord-009318-zt1o1bcz	306	32	m	m	NN
cord-009318-zt1o1bcz	306	33	.	.	.
cord-009318-zt1o1bcz	307	1	In	in	IN
cord-009318-zt1o1bcz	307	2	the	the	DT
cord-009318-zt1o1bcz	307	3	first	first	JJ
cord-009318-zt1o1bcz	307	4	12	12	CD
cord-009318-zt1o1bcz	307	5	min	min	NN
cord-009318-zt1o1bcz	307	6	of	of	IN
cord-009318-zt1o1bcz	307	7	amplification	amplification	NN
cord-009318-zt1o1bcz	307	8	,	,	,
cord-009318-zt1o1bcz	307	9	we	-PRON-	PRP
cord-009318-zt1o1bcz	307	10	observed	observe	VBD
cord-009318-zt1o1bcz	307	11	six	six	CD
cord-009318-zt1o1bcz	307	12	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	307	13	amplification	amplification	NN
cord-009318-zt1o1bcz	307	14	events	event	NNS
cord-009318-zt1o1bcz	307	15	in	in	IN
cord-009318-zt1o1bcz	307	16	Bst	Bst	NNP
cord-009318-zt1o1bcz	307	17	2.0	2.0	CD
cord-009318-zt1o1bcz	307	18	(	(	-LRB-
cord-009318-zt1o1bcz	307	19	four	four	CD
cord-009318-zt1o1bcz	307	20	in	in	IN
cord-009318-zt1o1bcz	307	21	the	the	DT
cord-009318-zt1o1bcz	307	22	presence	presence	NN
cord-009318-zt1o1bcz	307	23	of	of	IN
cord-009318-zt1o1bcz	307	24	template	template	NN
cord-009318-zt1o1bcz	307	25	;	;	:
cord-009318-zt1o1bcz	307	26	two	two	CD
cord-009318-zt1o1bcz	307	27	in	in	IN
cord-009318-zt1o1bcz	307	28	the	the	DT
cord-009318-zt1o1bcz	307	29	NTC	NTC	NNP
cord-009318-zt1o1bcz	307	30	;	;	:
cord-009318-zt1o1bcz	307	31	Figure	Figure	NNP
cord-009318-zt1o1bcz	307	32	6O	6o	CD
cord-009318-zt1o1bcz	307	33	)	)	-RRB-
cord-009318-zt1o1bcz	307	34	,	,	,
cord-009318-zt1o1bcz	307	35	and	and	CC
cord-009318-zt1o1bcz	307	36	we	-PRON-	PRP
cord-009318-zt1o1bcz	307	37	observed	observe	VBD
cord-009318-zt1o1bcz	307	38	13	13	CD
cord-009318-zt1o1bcz	307	39	non	non	JJ
cord-009318-zt1o1bcz	307	40	-	-	JJ
cord-009318-zt1o1bcz	307	41	specific	specific	JJ
cord-009318-zt1o1bcz	307	42	events	event	NNS
cord-009318-zt1o1bcz	307	43	in	in	IN
cord-009318-zt1o1bcz	307	44	Bst	Bst	NNP
cord-009318-zt1o1bcz	307	45	3.0	3.0	NNP
cord-009318-zt1o1bcz	307	46	(	(	-LRB-
cord-009318-zt1o1bcz	307	47	10	10	CD
cord-009318-zt1o1bcz	307	48	in	in	IN
cord-009318-zt1o1bcz	307	49	the	the	DT
cord-009318-zt1o1bcz	307	50	presence	presence	NN
cord-009318-zt1o1bcz	307	51	of	of	IN
cord-009318-zt1o1bcz	307	52	template	template	NN
cord-009318-zt1o1bcz	307	53	;	;	:
cord-009318-zt1o1bcz	307	54	three	three	CD
cord-009318-zt1o1bcz	307	55	in	in	IN
cord-009318-zt1o1bcz	307	56	the	the	DT
cord-009318-zt1o1bcz	307	57	NTC	NTC	NNP
cord-009318-zt1o1bcz	307	58	;	;	:
cord-009318-zt1o1bcz	307	59	Figure	figure	NN
cord-009318-zt1o1bcz	307	60	6P	6p	CD
cord-009318-zt1o1bcz	307	61	)	)	-RRB-
cord-009318-zt1o1bcz	307	62	.	.	.
cord-009318-zt1o1bcz	308	1	For	for	IN
cord-009318-zt1o1bcz	308	2	both	both	DT
cord-009318-zt1o1bcz	308	3	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	308	4	,	,	,
cord-009318-zt1o1bcz	308	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	308	6	were	be	VBD
cord-009318-zt1o1bcz	308	7	able	able	JJ
cord-009318-zt1o1bcz	308	8	to	to	TO
cord-009318-zt1o1bcz	308	9	distinguish	distinguish	VB
cord-009318-zt1o1bcz	308	10	the	the	DT
cord-009318-zt1o1bcz	308	11	rare	rare	JJ
cord-009318-zt1o1bcz	308	12	,	,	,
cord-009318-zt1o1bcz	308	13	fast	fast	JJ
cord-009318-zt1o1bcz	308	14	and	and	CC
cord-009318-zt1o1bcz	308	15	early	early	JJ
cord-009318-zt1o1bcz	308	16	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	308	17	amplicons	amplicon	NNS
cord-009318-zt1o1bcz	308	18	from	from	IN
cord-009318-zt1o1bcz	308	19	true	true	JJ
cord-009318-zt1o1bcz	308	20	positives	positive	NNS
cord-009318-zt1o1bcz	308	21	.	.	.
cord-009318-zt1o1bcz	309	1	For	for	IN
cord-009318-zt1o1bcz	309	2	Bst	Bst	NNP
cord-009318-zt1o1bcz	309	3	2.0	2.0	CD
cord-009318-zt1o1bcz	309	4	,	,	,
cord-009318-zt1o1bcz	309	5	these	these	DT
cord-009318-zt1o1bcz	309	6	non	non	JJ
cord-009318-zt1o1bcz	309	7	-	-	JJ
cord-009318-zt1o1bcz	309	8	specific	specific	JJ
cord-009318-zt1o1bcz	309	9	amplifications	amplification	NNS
cord-009318-zt1o1bcz	309	10	were	be	VBD
cord-009318-zt1o1bcz	309	11	slower	slow	JJR
cord-009318-zt1o1bcz	309	12	than	than	IN
cord-009318-zt1o1bcz	309	13	the	the	DT
cord-009318-zt1o1bcz	309	14	fastest	fast	JJS
cord-009318-zt1o1bcz	309	15	true	true	JJ
cord-009318-zt1o1bcz	309	16	positives	positive	NNS
cord-009318-zt1o1bcz	309	17	,	,	,
cord-009318-zt1o1bcz	309	18	and	and	CC
cord-009318-zt1o1bcz	309	19	occurred	occur	VBD
cord-009318-zt1o1bcz	309	20	at	at	IN
cord-009318-zt1o1bcz	309	21	similar	similar	JJ
cord-009318-zt1o1bcz	309	22	times	time	NNS
cord-009318-zt1o1bcz	309	23	.	.	.
cord-009318-zt1o1bcz	310	1	In	in	IN
cord-009318-zt1o1bcz	310	2	contrast	contrast	NN
cord-009318-zt1o1bcz	310	3	,	,	,
cord-009318-zt1o1bcz	310	4	for	for	IN
cord-009318-zt1o1bcz	310	5	Bst	Bst	NNP
cord-009318-zt1o1bcz	310	6	3.0	3.0	NNP
cord-009318-zt1o1bcz	310	7	,	,	,
cord-009318-zt1o1bcz	310	8	the	the	DT
cord-009318-zt1o1bcz	310	9	earliest	early	JJS
cord-009318-zt1o1bcz	310	10	amplification	amplification	NN
cord-009318-zt1o1bcz	310	11	events	event	NNS
cord-009318-zt1o1bcz	310	12	were	be	VBD
cord-009318-zt1o1bcz	310	13	false	false	JJ
cord-009318-zt1o1bcz	310	14	positives	positive	NNS
cord-009318-zt1o1bcz	310	15	and	and	CC
cord-009318-zt1o1bcz	310	16	tended	tend	VBD
cord-009318-zt1o1bcz	310	17	to	to	TO
cord-009318-zt1o1bcz	310	18	have	have	VB
cord-009318-zt1o1bcz	310	19	similar	similar	JJ
cord-009318-zt1o1bcz	310	20	rates	rate	NNS
cord-009318-zt1o1bcz	310	21	to	to	IN
cord-009318-zt1o1bcz	310	22	the	the	DT
cord-009318-zt1o1bcz	310	23	true	true	JJ
cord-009318-zt1o1bcz	310	24	positives	positive	NNS
cord-009318-zt1o1bcz	310	25	.	.	.
cord-009318-zt1o1bcz	311	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	311	2	hypothesize	hypothesize	VBP
cord-009318-zt1o1bcz	311	3	that	that	IN
cord-009318-zt1o1bcz	311	4	in	in	IN
cord-009318-zt1o1bcz	311	5	bulk	bulk	JJ
cord-009318-zt1o1bcz	311	6	reactions	reaction	NNS
cord-009318-zt1o1bcz	311	7	,	,	,
cord-009318-zt1o1bcz	311	8	the	the	DT
cord-009318-zt1o1bcz	311	9	fast	fast	JJ
cord-009318-zt1o1bcz	311	10	and	and	CC
cord-009318-zt1o1bcz	311	11	early	early	JJ
cord-009318-zt1o1bcz	311	12	non	non	JJ
cord-009318-zt1o1bcz	311	13	-	-	JJ
cord-009318-zt1o1bcz	311	14	specific	specific	JJ
cord-009318-zt1o1bcz	311	15	amplification	amplification	NN
cord-009318-zt1o1bcz	311	16	events	event	NNS
cord-009318-zt1o1bcz	311	17	(	(	-LRB-
cord-009318-zt1o1bcz	311	18	as	as	IN
cord-009318-zt1o1bcz	311	19	seen	see	VBN
cord-009318-zt1o1bcz	311	20	in	in	IN
cord-009318-zt1o1bcz	311	21	Bst	Bst	NNP
cord-009318-zt1o1bcz	311	22	3.0	3.0	CD
cord-009318-zt1o1bcz	311	23	Figure	figure	NN
cord-009318-zt1o1bcz	311	24	6P	6p	CD
cord-009318-zt1o1bcz	311	25	)	)	-RRB-
cord-009318-zt1o1bcz	311	26	lead	lead	NN
cord-009318-zt1o1bcz	311	27	to	to	IN
cord-009318-zt1o1bcz	311	28	non	non	JJ
cord-009318-zt1o1bcz	311	29	-	-	JJ
cord-009318-zt1o1bcz	311	30	specific	specific	JJ
cord-009318-zt1o1bcz	311	31	measurements	measurement	NNS
cord-009318-zt1o1bcz	311	32	,	,	,
cord-009318-zt1o1bcz	311	33	whereas	whereas	IN
cord-009318-zt1o1bcz	311	34	non	non	JJ
cord-009318-zt1o1bcz	311	35	-	-	JJ
cord-009318-zt1o1bcz	311	36	specific	specific	JJ
cord-009318-zt1o1bcz	311	37	amplification	amplification	NN
cord-009318-zt1o1bcz	311	38	that	that	WDT
cord-009318-zt1o1bcz	311	39	coincides	coincide	VBZ
cord-009318-zt1o1bcz	311	40	with	with	IN
cord-009318-zt1o1bcz	311	41	specific	specific	JJ
cord-009318-zt1o1bcz	311	42	amplification	amplification	NN
cord-009318-zt1o1bcz	311	43	,	,	,
cord-009318-zt1o1bcz	311	44	but	but	CC
cord-009318-zt1o1bcz	311	45	proceeds	proceed	VBZ
cord-009318-zt1o1bcz	311	46	at	at	IN
cord-009318-zt1o1bcz	311	47	a	a	DT
cord-009318-zt1o1bcz	311	48	slower	slow	JJR
cord-009318-zt1o1bcz	311	49	rate	rate	NN
cord-009318-zt1o1bcz	311	50	(	(	-LRB-
cord-009318-zt1o1bcz	311	51	as	as	IN
cord-009318-zt1o1bcz	311	52	seen	see	VBN
cord-009318-zt1o1bcz	311	53	in	in	IN
cord-009318-zt1o1bcz	311	54	Bst	Bst	NNP
cord-009318-zt1o1bcz	311	55	2.0	2.0	CD
cord-009318-zt1o1bcz	311	56	Figure	figure	NN
cord-009318-zt1o1bcz	311	57	6O	6o	CD
cord-009318-zt1o1bcz	311	58	)	)	-RRB-
cord-009318-zt1o1bcz	311	59	,	,	,
cord-009318-zt1o1bcz	311	60	would	would	MD
cord-009318-zt1o1bcz	311	61	still	still	RB
cord-009318-zt1o1bcz	311	62	produce	produce	VB
cord-009318-zt1o1bcz	311	63	specific	specific	JJ
cord-009318-zt1o1bcz	311	64	amplification	amplification	NN
cord-009318-zt1o1bcz	311	65	in	in	IN
cord-009318-zt1o1bcz	311	66	bulk	bulk	NN
cord-009318-zt1o1bcz	311	67	.	.	.
cord-009318-zt1o1bcz	312	1	This	this	DT
cord-009318-zt1o1bcz	312	2	hypothesis	hypothesis	NN
cord-009318-zt1o1bcz	312	3	is	be	VBZ
cord-009318-zt1o1bcz	312	4	corroborated	corroborate	VBN
cord-009318-zt1o1bcz	312	5	by	by	IN
cord-009318-zt1o1bcz	312	6	sequencing	sequencing	NN
cord-009318-zt1o1bcz	312	7	of	of	IN
cord-009318-zt1o1bcz	312	8	bulk	bulk	JJ
cord-009318-zt1o1bcz	312	9	LAMP	lamp	NN
cord-009318-zt1o1bcz	312	10	reactions	reaction	NNS
cord-009318-zt1o1bcz	312	11	(	(	-LRB-
cord-009318-zt1o1bcz	312	12	Figure	figure	NN
cord-009318-zt1o1bcz	312	13	2	2	CD
cord-009318-zt1o1bcz	312	14	)	)	-RRB-
cord-009318-zt1o1bcz	312	15	.	.	.
cord-009318-zt1o1bcz	313	1	Though	though	IN
cord-009318-zt1o1bcz	313	2	individual	individual	JJ
cord-009318-zt1o1bcz	313	3	bulk	bulk	JJ
cord-009318-zt1o1bcz	313	4	reactions	reaction	NNS
cord-009318-zt1o1bcz	313	5	may	may	MD
cord-009318-zt1o1bcz	313	6	be	be	VB
cord-009318-zt1o1bcz	313	7	assigned	assign	VBN
cord-009318-zt1o1bcz	313	8	a	a	DT
cord-009318-zt1o1bcz	313	9	homogeneous	homogeneous	JJ
cord-009318-zt1o1bcz	313	10	label	label	NN
cord-009318-zt1o1bcz	313	11	as	as	IN
cord-009318-zt1o1bcz	313	12	'	'	``
cord-009318-zt1o1bcz	313	13	true	true	JJ
cord-009318-zt1o1bcz	313	14	positive	positive	JJ
cord-009318-zt1o1bcz	313	15	'	'	''
cord-009318-zt1o1bcz	313	16	or	or	CC
cord-009318-zt1o1bcz	313	17	'	'	``
cord-009318-zt1o1bcz	313	18	false	false	JJ
cord-009318-zt1o1bcz	313	19	positive	positive	JJ
cord-009318-zt1o1bcz	313	20	'	'	''
cord-009318-zt1o1bcz	313	21	by	by	IN
cord-009318-zt1o1bcz	313	22	T	T	NNP
cord-009318-zt1o1bcz	313	23	m	m	NNP
cord-009318-zt1o1bcz	313	24	,	,	,
cord-009318-zt1o1bcz	313	25	sequencing	sequencing	NN
cord-009318-zt1o1bcz	313	26	identifies	identify	VBZ
cord-009318-zt1o1bcz	313	27	multiple	multiple	JJ
cord-009318-zt1o1bcz	313	28	products	product	NNS
cord-009318-zt1o1bcz	313	29	within	within	IN
cord-009318-zt1o1bcz	313	30	each	each	DT
cord-009318-zt1o1bcz	313	31	reaction	reaction	NN
cord-009318-zt1o1bcz	313	32	and	and	CC
cord-009318-zt1o1bcz	313	33	the	the	DT
cord-009318-zt1o1bcz	313	34	T	t	NN
cord-009318-zt1o1bcz	313	35	m	m	NNP
cord-009318-zt1o1bcz	313	36	is	be	VBZ
cord-009318-zt1o1bcz	313	37	determined	determine	VBN
cord-009318-zt1o1bcz	313	38	by	by	IN
cord-009318-zt1o1bcz	313	39	the	the	DT
cord-009318-zt1o1bcz	313	40	dominant	dominant	JJ
cord-009318-zt1o1bcz	313	41	product	product	NN
cord-009318-zt1o1bcz	313	42	.	.	.
cord-009318-zt1o1bcz	314	1	For	for	IN
cord-009318-zt1o1bcz	314	2	example	example	NN
cord-009318-zt1o1bcz	314	3	,	,	,
cord-009318-zt1o1bcz	314	4	we	-PRON-	PRP
cord-009318-zt1o1bcz	314	5	observed	observe	VBD
cord-009318-zt1o1bcz	314	6	a	a	DT
cord-009318-zt1o1bcz	314	7	'	'	``
cord-009318-zt1o1bcz	314	8	false	false	JJ
cord-009318-zt1o1bcz	314	9	positive	positive	NN
cord-009318-zt1o1bcz	314	10	'	'	''
cord-009318-zt1o1bcz	314	11	by	by	IN
cord-009318-zt1o1bcz	314	12	T	T	NNP
cord-009318-zt1o1bcz	314	13	m	m	NNP
cord-009318-zt1o1bcz	314	14	(	(	-LRB-
cord-009318-zt1o1bcz	314	15	Figure	Figure	NNP
cord-009318-zt1o1bcz	314	16	 	 	_SP
cord-009318-zt1o1bcz	314	17	1C	1c	NN
cord-009318-zt1o1bcz	314	18	and	and	CC
cord-009318-zt1o1bcz	314	19	D	D	NNP
cord-009318-zt1o1bcz	314	20	)	)	-RRB-
cord-009318-zt1o1bcz	314	21	,	,	,
cord-009318-zt1o1bcz	314	22	despite	despite	IN
cord-009318-zt1o1bcz	314	23	the	the	DT
cord-009318-zt1o1bcz	314	24	presence	presence	NN
cord-009318-zt1o1bcz	314	25	of	of	IN
cord-009318-zt1o1bcz	314	26	template	template	NN
cord-009318-zt1o1bcz	314	27	.	.	.
cord-009318-zt1o1bcz	315	1	The	the	DT
cord-009318-zt1o1bcz	315	2	sequencing	sequencing	NN
cord-009318-zt1o1bcz	315	3	of	of	IN
cord-009318-zt1o1bcz	315	4	this	this	DT
cord-009318-zt1o1bcz	315	5	product	product	NN
cord-009318-zt1o1bcz	315	6	,	,	,
cord-009318-zt1o1bcz	315	7	contained	contain	VBD
cord-009318-zt1o1bcz	315	8	non	non	JJ
cord-009318-zt1o1bcz	315	9	-	-	JJ
cord-009318-zt1o1bcz	315	10	specific	specific	JJ
cord-009318-zt1o1bcz	315	11	product	product	NN
cord-009318-zt1o1bcz	315	12	sequences	sequence	NNS
cord-009318-zt1o1bcz	315	13	,	,	,
cord-009318-zt1o1bcz	315	14	similar	similar	JJ
cord-009318-zt1o1bcz	315	15	to	to	IN
cord-009318-zt1o1bcz	315	16	those	those	DT
cord-009318-zt1o1bcz	315	17	observed	observe	VBN
cord-009318-zt1o1bcz	315	18	in	in	IN
cord-009318-zt1o1bcz	315	19	the	the	DT
cord-009318-zt1o1bcz	315	20	NTC	NTC	NNP
cord-009318-zt1o1bcz	315	21	,	,	,
cord-009318-zt1o1bcz	315	22	at	at	IN
cord-009318-zt1o1bcz	315	23	high	high	JJ
cord-009318-zt1o1bcz	315	24	prevalence	prevalence	NN
cord-009318-zt1o1bcz	315	25	,	,	,
cord-009318-zt1o1bcz	315	26	as	as	RB
cord-009318-zt1o1bcz	315	27	well	well	RB
cord-009318-zt1o1bcz	315	28	as	as	IN
cord-009318-zt1o1bcz	315	29	the	the	DT
cord-009318-zt1o1bcz	315	30	specific	specific	JJ
cord-009318-zt1o1bcz	315	31	target	target	NN
cord-009318-zt1o1bcz	315	32	sequences	sequence	NNS
cord-009318-zt1o1bcz	315	33	in	in	IN
cord-009318-zt1o1bcz	315	34	low	low	JJ
cord-009318-zt1o1bcz	315	35	abundance	abundance	NN
cord-009318-zt1o1bcz	316	1	(	(	-LRB-
cord-009318-zt1o1bcz	316	2	Figure	figure	NN
cord-009318-zt1o1bcz	316	3	2	2	CD
cord-009318-zt1o1bcz	316	4	[	[	-LRB-
cord-009318-zt1o1bcz	316	5	well	well	UH
cord-009318-zt1o1bcz	316	6	C7	C7	NNP
cord-009318-zt1o1bcz	316	7	]	]	-RRB-
cord-009318-zt1o1bcz	316	8	)	)	-RRB-
cord-009318-zt1o1bcz	316	9	.	.	.
cord-009318-zt1o1bcz	317	1	Similarly	similarly	RB
cord-009318-zt1o1bcz	317	2	,	,	,
cord-009318-zt1o1bcz	317	3	though	though	IN
cord-009318-zt1o1bcz	317	4	'	'	``
cord-009318-zt1o1bcz	317	5	true	true	JJ
cord-009318-zt1o1bcz	317	6	positive	positive	JJ
cord-009318-zt1o1bcz	317	7	'	'	''
cord-009318-zt1o1bcz	317	8	is	be	VBZ
cord-009318-zt1o1bcz	317	9	as	as	RB
cord-009318-zt1o1bcz	317	10	-	-	HYPH
cord-009318-zt1o1bcz	317	11	signed	sign	VBN
cord-009318-zt1o1bcz	317	12	to	to	IN
cord-009318-zt1o1bcz	317	13	other	other	JJ
cord-009318-zt1o1bcz	317	14	bulk	bulk	JJ
cord-009318-zt1o1bcz	317	15	reactions	reaction	NNS
cord-009318-zt1o1bcz	317	16	in	in	IN
cord-009318-zt1o1bcz	317	17	the	the	DT
cord-009318-zt1o1bcz	317	18	presence	presence	NN
cord-009318-zt1o1bcz	317	19	of	of	IN
cord-009318-zt1o1bcz	317	20	template	template	NN
cord-009318-zt1o1bcz	317	21	,	,	,
cord-009318-zt1o1bcz	317	22	the	the	DT
cord-009318-zt1o1bcz	317	23	non	non	JJ
cord-009318-zt1o1bcz	317	24	-	-	JJ
cord-009318-zt1o1bcz	317	25	specific	specific	JJ
cord-009318-zt1o1bcz	317	26	products	product	NNS
cord-009318-zt1o1bcz	317	27	are	be	VBP
cord-009318-zt1o1bcz	317	28	still	still	RB
cord-009318-zt1o1bcz	317	29	observed	observe	VBN
cord-009318-zt1o1bcz	317	30	at	at	IN
cord-009318-zt1o1bcz	317	31	low	low	JJ
cord-009318-zt1o1bcz	317	32	abundance	abundance	NN
cord-009318-zt1o1bcz	317	33	(	(	-LRB-
cord-009318-zt1o1bcz	317	34	e.g.	e.g.	JJ
cord-009318-zt1o1bcz	317	35	Figure	figure	NN
cord-009318-zt1o1bcz	317	36	2	2	CD
cord-009318-zt1o1bcz	317	37	[	[	-LRB-
cord-009318-zt1o1bcz	317	38	well	well	UH
cord-009318-zt1o1bcz	317	39	F8	f8	NN
cord-009318-zt1o1bcz	317	40	]	]	-RRB-
cord-009318-zt1o1bcz	317	41	)	)	-RRB-
cord-009318-zt1o1bcz	317	42	.	.	.
cord-009318-zt1o1bcz	318	1	Further	further	RB
cord-009318-zt1o1bcz	318	2	,	,	,
cord-009318-zt1o1bcz	318	3	a	a	DT
cord-009318-zt1o1bcz	318	4	greater	great	JJR
cord-009318-zt1o1bcz	318	5	number	number	NN
cord-009318-zt1o1bcz	318	6	of	of	IN
cord-009318-zt1o1bcz	318	7	non	non	JJ
cord-009318-zt1o1bcz	318	8	-	-	JJ
cord-009318-zt1o1bcz	318	9	specific	specific	JJ
cord-009318-zt1o1bcz	318	10	partitions	partition	NNS
cord-009318-zt1o1bcz	318	11	in	in	IN
cord-009318-zt1o1bcz	318	12	digital	digital	JJ
cord-009318-zt1o1bcz	318	13	using	use	VBG
cord-009318-zt1o1bcz	318	14	Bst	Bst	NNP
cord-009318-zt1o1bcz	318	15	3.0	3.0	NNP
cord-009318-zt1o1bcz	318	16	than	than	IN
cord-009318-zt1o1bcz	318	17	Bst	Bst	NNP
cord-009318-zt1o1bcz	318	18	2.0	2.0	NNP
cord-009318-zt1o1bcz	318	19	,	,	,
cord-009318-zt1o1bcz	318	20	is	be	VBZ
cord-009318-zt1o1bcz	318	21	correlated	correlate	VBN
cord-009318-zt1o1bcz	318	22	with	with	IN
cord-009318-zt1o1bcz	318	23	a	a	DT
cord-009318-zt1o1bcz	318	24	greater	great	JJR
cord-009318-zt1o1bcz	318	25	number	number	NN
cord-009318-zt1o1bcz	318	26	of	of	IN
cord-009318-zt1o1bcz	318	27	non	non	JJ
cord-009318-zt1o1bcz	318	28	-	-	JJ
cord-009318-zt1o1bcz	318	29	specific	specific	JJ
cord-009318-zt1o1bcz	318	30	reads	read	NNS
cord-009318-zt1o1bcz	318	31	despite	despite	IN
cord-009318-zt1o1bcz	318	32	the	the	DT
cord-009318-zt1o1bcz	318	33	presence	presence	NN
cord-009318-zt1o1bcz	318	34	of	of	IN
cord-009318-zt1o1bcz	318	35	template	template	NN
cord-009318-zt1o1bcz	318	36	in	in	IN
cord-009318-zt1o1bcz	318	37	the	the	DT
cord-009318-zt1o1bcz	318	38	sequencing	sequencing	NN
cord-009318-zt1o1bcz	318	39	data	datum	NNS
cord-009318-zt1o1bcz	318	40	(	(	-LRB-
cord-009318-zt1o1bcz	318	41	Comparing	compare	VBG
cord-009318-zt1o1bcz	318	42	Figures	figure	NNS
cord-009318-zt1o1bcz	318	43	6A	6A	NNP
cord-009318-zt1o1bcz	318	44	-	-	HYPH
cord-009318-zt1o1bcz	318	45	B	b	NN
cord-009318-zt1o1bcz	318	46	and	and	CC
cord-009318-zt1o1bcz	318	47	2B	2b	CD
cord-009318-zt1o1bcz	318	48	group	group	NN
cord-009318-zt1o1bcz	318	49	A	A	NNP
cord-009318-zt1o1bcz	318	50	versus	versus	IN
cord-009318-zt1o1bcz	318	51	C	C	NNP
cord-009318-zt1o1bcz	318	52	)	)	-RRB-
cord-009318-zt1o1bcz	318	53	.	.	.
cord-009318-zt1o1bcz	319	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	319	2	hypothesize	hypothesize	VBP
cord-009318-zt1o1bcz	319	3	that	that	IN
cord-009318-zt1o1bcz	319	4	the	the	DT
cord-009318-zt1o1bcz	319	5	combination	combination	NN
cord-009318-zt1o1bcz	319	6	of	of	IN
cord-009318-zt1o1bcz	319	7	real	real	JJ
cord-009318-zt1o1bcz	319	8	-	-	HYPH
cord-009318-zt1o1bcz	319	9	time	time	NN
cord-009318-zt1o1bcz	319	10	parameters	parameter	NNS
cord-009318-zt1o1bcz	319	11	(	(	-LRB-
cord-009318-zt1o1bcz	319	12	such	such	JJ
cord-009318-zt1o1bcz	319	13	as	as	IN
cord-009318-zt1o1bcz	319	14	rate	rate	NN
cord-009318-zt1o1bcz	319	15	and	and	CC
cord-009318-zt1o1bcz	319	16	TTP	TTP	NNP
cord-009318-zt1o1bcz	319	17	)	)	-RRB-
cord-009318-zt1o1bcz	319	18	,	,	,
cord-009318-zt1o1bcz	319	19	combined	combine	VBN
cord-009318-zt1o1bcz	319	20	with	with	IN
cord-009318-zt1o1bcz	319	21	the	the	DT
cord-009318-zt1o1bcz	319	22	ability	ability	NN
cord-009318-zt1o1bcz	319	23	of	of	IN
cord-009318-zt1o1bcz	319	24	digital	digital	JJ
cord-009318-zt1o1bcz	319	25	assays	assay	NNS
cord-009318-zt1o1bcz	319	26	to	to	TO
cord-009318-zt1o1bcz	319	27	yield	yield	VB
cord-009318-zt1o1bcz	319	28	probabilities	probability	NNS
cord-009318-zt1o1bcz	319	29	and	and	CC
cord-009318-zt1o1bcz	319	30	to	to	TO
cord-009318-zt1o1bcz	319	31	assign	assign	VB
cord-009318-zt1o1bcz	319	32	reaction	reaction	NN
cord-009318-zt1o1bcz	319	33	identity	identity	NN
cord-009318-zt1o1bcz	319	34	through	through	IN
cord-009318-zt1o1bcz	319	35	HRM	HRM	NNP
cord-009318-zt1o1bcz	319	36	,	,	,
cord-009318-zt1o1bcz	319	37	may	may	MD
cord-009318-zt1o1bcz	319	38	ultimately	ultimately	RB
cord-009318-zt1o1bcz	319	39	help	help	VB
cord-009318-zt1o1bcz	319	40	researchers	researcher	NNS
cord-009318-zt1o1bcz	319	41	optimize	optimize	VB
cord-009318-zt1o1bcz	319	42	bulk	bulk	JJ
cord-009318-zt1o1bcz	319	43	reaction	reaction	NN
cord-009318-zt1o1bcz	319	44	conditions	condition	NNS
cord-009318-zt1o1bcz	319	45	.	.	.
cord-009318-zt1o1bcz	320	1	To	to	TO
cord-009318-zt1o1bcz	320	2	better	better	RB
cord-009318-zt1o1bcz	320	3	visualize	visualize	VB
cord-009318-zt1o1bcz	320	4	how	how	WRB
cord-009318-zt1o1bcz	320	5	TTP	TTP	NNP
cord-009318-zt1o1bcz	320	6	,	,	,
cord-009318-zt1o1bcz	320	7	max	max	NN
cord-009318-zt1o1bcz	320	8	rate	rate	NN
cord-009318-zt1o1bcz	320	9	,	,	,
cord-009318-zt1o1bcz	320	10	final	final	JJ
cord-009318-zt1o1bcz	320	11	intensity	intensity	NN
cord-009318-zt1o1bcz	320	12	and	and	CC
cord-009318-zt1o1bcz	320	13	T	T	NNP
cord-009318-zt1o1bcz	320	14	m	m	NNP
cord-009318-zt1o1bcz	320	15	data	datum	NNS
cord-009318-zt1o1bcz	320	16	are	be	VBP
cord-009318-zt1o1bcz	320	17	interrelated	interrelated	JJ
cord-009318-zt1o1bcz	320	18	,	,	,
cord-009318-zt1o1bcz	320	19	we	-PRON-	PRP
cord-009318-zt1o1bcz	320	20	next	next	RB
cord-009318-zt1o1bcz	320	21	plotted	plot	VBD
cord-009318-zt1o1bcz	320	22	these	these	DT
cord-009318-zt1o1bcz	320	23	data	datum	NNS
cord-009318-zt1o1bcz	320	24	in	in	IN
cord-009318-zt1o1bcz	320	25	a	a	DT
cord-009318-zt1o1bcz	320	26	four	four	CD
cord-009318-zt1o1bcz	320	27	-	-	HYPH
cord-009318-zt1o1bcz	320	28	dimensional	dimensional	JJ
cord-009318-zt1o1bcz	320	29	(	(	-LRB-
cord-009318-zt1o1bcz	320	30	4D	4D	NNP
cord-009318-zt1o1bcz	320	31	)	)	-RRB-
cord-009318-zt1o1bcz	320	32	space	space	NN
cord-009318-zt1o1bcz	320	33	(	(	-LRB-
cord-009318-zt1o1bcz	320	34	Figure	figure	NN
cord-009318-zt1o1bcz	320	35	6Q	6q	CD
cord-009318-zt1o1bcz	320	36	-R	-R	NNPS
cord-009318-zt1o1bcz	320	37	,	,	,
cord-009318-zt1o1bcz	320	38	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	320	39	Videos	Videos	NNPS
cord-009318-zt1o1bcz	320	40	S1	S1	NNP
cord-009318-zt1o1bcz	320	41	and	and	CC
cord-009318-zt1o1bcz	320	42	2	2	CD
cord-009318-zt1o1bcz	320	43	)	)	-RRB-
cord-009318-zt1o1bcz	320	44	.	.	.
cord-009318-zt1o1bcz	321	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	321	2	observed	observe	VBD
cord-009318-zt1o1bcz	321	3	that	that	IN
cord-009318-zt1o1bcz	321	4	among	among	IN
cord-009318-zt1o1bcz	321	5	all	all	DT
cord-009318-zt1o1bcz	321	6	partitions	partition	NNS
cord-009318-zt1o1bcz	321	7	,	,	,
cord-009318-zt1o1bcz	321	8	regarless	regarless	NN
cord-009318-zt1o1bcz	321	9	of	of	IN
cord-009318-zt1o1bcz	321	10	if	if	IN
cord-009318-zt1o1bcz	321	11	the	the	DT
cord-009318-zt1o1bcz	321	12	product	product	NN
cord-009318-zt1o1bcz	321	13	was	be	VBD
cord-009318-zt1o1bcz	321	14	specific	specific	JJ
cord-009318-zt1o1bcz	321	15	or	or	CC
cord-009318-zt1o1bcz	321	16	non	non	JJ
cord-009318-zt1o1bcz	321	17	-	-	JJ
cord-009318-zt1o1bcz	321	18	specific	specific	JJ
cord-009318-zt1o1bcz	321	19	amplificiation	amplificiation	NN
cord-009318-zt1o1bcz	321	20	,	,	,
cord-009318-zt1o1bcz	321	21	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	321	22	was	be	VBD
cord-009318-zt1o1bcz	321	23	brighter	bright	JJR
cord-009318-zt1o1bcz	321	24	when	when	WRB
cord-009318-zt1o1bcz	321	25	amplification	amplification	NN
cord-009318-zt1o1bcz	321	26	occurred	occur	VBD
cord-009318-zt1o1bcz	321	27	earlier	early	RBR
cord-009318-zt1o1bcz	321	28	and	and	CC
cord-009318-zt1o1bcz	321	29	faster	fast	RBR
cord-009318-zt1o1bcz	321	30	.	.	.
cord-009318-zt1o1bcz	322	1	This	this	DT
cord-009318-zt1o1bcz	322	2	was	be	VBD
cord-009318-zt1o1bcz	322	3	true	true	JJ
cord-009318-zt1o1bcz	322	4	for	for	IN
cord-009318-zt1o1bcz	322	5	both	both	DT
cord-009318-zt1o1bcz	322	6	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	322	7	.	.	.
cord-009318-zt1o1bcz	323	1	Additionally	additionally	RB
cord-009318-zt1o1bcz	323	2	,	,	,
cord-009318-zt1o1bcz	323	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	323	4	observed	observe	VBD
cord-009318-zt1o1bcz	323	5	two	two	CD
cord-009318-zt1o1bcz	323	6	types	type	NNS
cord-009318-zt1o1bcz	323	7	of	of	IN
cord-009318-zt1o1bcz	323	8	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	323	9	amplification	amplification	NN
cord-009318-zt1o1bcz	323	10	.	.	.
cord-009318-zt1o1bcz	324	1	The	the	DT
cord-009318-zt1o1bcz	324	2	first	first	JJ
cord-009318-zt1o1bcz	324	3	type	type	NN
cord-009318-zt1o1bcz	324	4	of	of	IN
cord-009318-zt1o1bcz	324	5	non	non	JJ
cord-009318-zt1o1bcz	324	6	-	-	JJ
cord-009318-zt1o1bcz	324	7	specific	specific	JJ
cord-009318-zt1o1bcz	324	8	was	be	VBD
cord-009318-zt1o1bcz	324	9	the	the	DT
cord-009318-zt1o1bcz	324	10	traditional	traditional	JJ
cord-009318-zt1o1bcz	324	11	'	'	``
cord-009318-zt1o1bcz	324	12	primer	primer	NN
cord-009318-zt1o1bcz	324	13	-	-	HYPH
cord-009318-zt1o1bcz	324	14	dimer	dimer	NN
cord-009318-zt1o1bcz	324	15	'	'	''
cord-009318-zt1o1bcz	324	16	cloud	cloud	NN
cord-009318-zt1o1bcz	324	17	,	,	,
cord-009318-zt1o1bcz	324	18	which	which	WDT
cord-009318-zt1o1bcz	324	19	is	be	VBZ
cord-009318-zt1o1bcz	324	20	characterized	characterize	VBN
cord-009318-zt1o1bcz	324	21	by	by	IN
cord-009318-zt1o1bcz	324	22	a	a	DT
cord-009318-zt1o1bcz	324	23	low	low	JJ
cord-009318-zt1o1bcz	324	24	T	t	NN
cord-009318-zt1o1bcz	324	25	m	m	NN
cord-009318-zt1o1bcz	324	26	,	,	,
cord-009318-zt1o1bcz	324	27	low	low	JJ
cord-009318-zt1o1bcz	324	28	final	final	JJ
cord-009318-zt1o1bcz	324	29	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	324	30	intensity	intensity	NN
cord-009318-zt1o1bcz	324	31	,	,	,
cord-009318-zt1o1bcz	324	32	a	a	DT
cord-009318-zt1o1bcz	324	33	slow	slow	JJ
cord-009318-zt1o1bcz	324	34	max	max	NN
cord-009318-zt1o1bcz	324	35	rate	rate	NN
cord-009318-zt1o1bcz	324	36	and	and	CC
cord-009318-zt1o1bcz	324	37	a	a	DT
cord-009318-zt1o1bcz	324	38	generally	generally	RB
cord-009318-zt1o1bcz	324	39	late	late	JJ
cord-009318-zt1o1bcz	324	40	TTP	ttp	NN
cord-009318-zt1o1bcz	324	41	.	.	.
cord-009318-zt1o1bcz	325	1	The	the	DT
cord-009318-zt1o1bcz	325	2	second	second	JJ
cord-009318-zt1o1bcz	325	3	type	type	NN
cord-009318-zt1o1bcz	325	4	of	of	IN
cord-009318-zt1o1bcz	325	5	non	non	JJ
cord-009318-zt1o1bcz	325	6	-	-	JJ
cord-009318-zt1o1bcz	325	7	specific	specific	JJ
cord-009318-zt1o1bcz	325	8	cloud	cloud	NN
cord-009318-zt1o1bcz	325	9	matches	match	NNS
cord-009318-zt1o1bcz	325	10	only	only	RB
cord-009318-zt1o1bcz	325	11	in	in	IN
cord-009318-zt1o1bcz	325	12	its	-PRON-	PRP$
cord-009318-zt1o1bcz	325	13	high	high	JJ
cord-009318-zt1o1bcz	325	14	T	t	NN
cord-009318-zt1o1bcz	325	15	m	m	NN
cord-009318-zt1o1bcz	325	16	,	,	,
cord-009318-zt1o1bcz	325	17	and	and	CC
cord-009318-zt1o1bcz	325	18	spans	span	VBZ
cord-009318-zt1o1bcz	325	19	a	a	DT
cord-009318-zt1o1bcz	325	20	wide	wide	JJ
cord-009318-zt1o1bcz	325	21	range	range	NN
cord-009318-zt1o1bcz	325	22	of	of	IN
cord-009318-zt1o1bcz	325	23	rates	rate	NNS
cord-009318-zt1o1bcz	325	24	,	,	,
cord-009318-zt1o1bcz	325	25	TTP	ttp	NN
cord-009318-zt1o1bcz	325	26	and	and	CC
cord-009318-zt1o1bcz	325	27	final	final	JJ
cord-009318-zt1o1bcz	325	28	intensities	intensity	NNS
cord-009318-zt1o1bcz	325	29	.	.	.
cord-009318-zt1o1bcz	326	1	The	the	DT
cord-009318-zt1o1bcz	326	2	high	high	JJ
cord-009318-zt1o1bcz	326	3	-	-	HYPH
cord-009318-zt1o1bcz	326	4	T	t	NN
cord-009318-zt1o1bcz	326	5	m	m	NN
cord-009318-zt1o1bcz	326	6	non	non	JJ
cord-009318-zt1o1bcz	326	7	-	-	JJ
cord-009318-zt1o1bcz	326	8	specific	specific	JJ
cord-009318-zt1o1bcz	326	9	amplification	amplification	NN
cord-009318-zt1o1bcz	326	10	occurs	occur	VBZ
cord-009318-zt1o1bcz	326	11	with	with	IN
cord-009318-zt1o1bcz	326	12	greater	great	JJR
cord-009318-zt1o1bcz	326	13	frequency	frequency	NN
cord-009318-zt1o1bcz	326	14	than	than	IN
cord-009318-zt1o1bcz	326	15	the	the	DT
cord-009318-zt1o1bcz	326	16	low	low	JJ
cord-009318-zt1o1bcz	326	17	-	-	HYPH
cord-009318-zt1o1bcz	326	18	T	t	NN
cord-009318-zt1o1bcz	326	19	m	m	NN
cord-009318-zt1o1bcz	326	20	non	non	JJ
cord-009318-zt1o1bcz	326	21	-	-	JJ
cord-009318-zt1o1bcz	326	22	specific	specific	JJ
cord-009318-zt1o1bcz	326	23	amplification	amplification	NN
cord-009318-zt1o1bcz	326	24	.	.	.
cord-009318-zt1o1bcz	327	1	The	the	DT
cord-009318-zt1o1bcz	327	2	major	major	JJ
cord-009318-zt1o1bcz	327	3	differences	difference	NNS
cord-009318-zt1o1bcz	327	4	between	between	IN
cord-009318-zt1o1bcz	327	5	the	the	DT
cord-009318-zt1o1bcz	327	6	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	327	7	can	can	MD
cord-009318-zt1o1bcz	327	8	also	also	RB
cord-009318-zt1o1bcz	327	9	be	be	VB
cord-009318-zt1o1bcz	327	10	resolved	resolve	VBN
cord-009318-zt1o1bcz	327	11	with	with	IN
cord-009318-zt1o1bcz	327	12	this	this	DT
cord-009318-zt1o1bcz	327	13	visualization	visualization	NN
cord-009318-zt1o1bcz	327	14	.	.	.
cord-009318-zt1o1bcz	328	1	The	the	DT
cord-009318-zt1o1bcz	328	2	number	number	NN
cord-009318-zt1o1bcz	328	3	of	of	IN
cord-009318-zt1o1bcz	328	4	non	non	JJ
cord-009318-zt1o1bcz	328	5	-	-	JJ
cord-009318-zt1o1bcz	328	6	specific	specific	JJ
cord-009318-zt1o1bcz	328	7	amplification	amplification	NN
cord-009318-zt1o1bcz	328	8	events	event	NNS
cord-009318-zt1o1bcz	328	9	is	be	VBZ
cord-009318-zt1o1bcz	328	10	much	much	RB
cord-009318-zt1o1bcz	328	11	fewer	few	JJR
cord-009318-zt1o1bcz	328	12	for	for	IN
cord-009318-zt1o1bcz	328	13	Bst	Bst	NNP
cord-009318-zt1o1bcz	328	14	2.0	2.0	CD
cord-009318-zt1o1bcz	328	15	than	than	IN
cord-009318-zt1o1bcz	328	16	for	for	IN
cord-009318-zt1o1bcz	328	17	Bst	Bst	NNP
cord-009318-zt1o1bcz	328	18	3.0	3.0	NNP
cord-009318-zt1o1bcz	328	19	.	.	.
cord-009318-zt1o1bcz	329	1	Further	further	RB
cord-009318-zt1o1bcz	329	2	,	,	,
cord-009318-zt1o1bcz	329	3	these	these	DT
cord-009318-zt1o1bcz	329	4	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	329	5	events	event	NNS
cord-009318-zt1o1bcz	329	6	in	in	IN
cord-009318-zt1o1bcz	329	7	Bst	Bst	NNP
cord-009318-zt1o1bcz	329	8	2.0	2.0	NNP
cord-009318-zt1o1bcz	329	9	never	never	RB
cord-009318-zt1o1bcz	329	10	achieve	achieve	VBP
cord-009318-zt1o1bcz	329	11	same	same	JJ
cord-009318-zt1o1bcz	329	12	fluorescene	fluorescene	JJ
cord-009318-zt1o1bcz	329	13	intensity	intensity	NN
cord-009318-zt1o1bcz	329	14	or	or	CC
cord-009318-zt1o1bcz	329	15	maximum	maximum	JJ
cord-009318-zt1o1bcz	329	16	rate	rate	NN
cord-009318-zt1o1bcz	329	17	as	as	IN
cord-009318-zt1o1bcz	329	18	with	with	IN
cord-009318-zt1o1bcz	329	19	Bst	Bst	NNP
cord-009318-zt1o1bcz	329	20	3.0	3.0	NNP
cord-009318-zt1o1bcz	329	21	.	.	.
cord-009318-zt1o1bcz	330	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	330	2	include	include	VBP
cord-009318-zt1o1bcz	330	3	the	the	DT
cord-009318-zt1o1bcz	330	4	4D	4d	CD
cord-009318-zt1o1bcz	330	5	representation	representation	NN
cord-009318-zt1o1bcz	330	6	as	as	IN
cord-009318-zt1o1bcz	330	7	part	part	NN
cord-009318-zt1o1bcz	330	8	of	of	IN
cord-009318-zt1o1bcz	330	9	our	-PRON-	PRP$
cord-009318-zt1o1bcz	330	10	MATLAB	MATLAB	NNP
cord-009318-zt1o1bcz	330	11	code	code	NN
cord-009318-zt1o1bcz	330	12	,	,	,
cord-009318-zt1o1bcz	330	13	and	and	CC
cord-009318-zt1o1bcz	330	14	as	as	IN
cord-009318-zt1o1bcz	330	15	videos	video	NNS
cord-009318-zt1o1bcz	330	16	in	in	IN
cord-009318-zt1o1bcz	330	17	the	the	DT
cord-009318-zt1o1bcz	330	18	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	330	19	Data	Data	NNP
cord-009318-zt1o1bcz	330	20	.	.	.
cord-009318-zt1o1bcz	331	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	331	2	next	next	RB
cord-009318-zt1o1bcz	331	3	asked	ask	VBD
cord-009318-zt1o1bcz	331	4	whether	whether	IN
cord-009318-zt1o1bcz	331	5	using	use	VBG
cord-009318-zt1o1bcz	331	6	a	a	DT
cord-009318-zt1o1bcz	331	7	combination	combination	NN
cord-009318-zt1o1bcz	331	8	of	of	IN
cord-009318-zt1o1bcz	331	9	digital	digital	JJ
cord-009318-zt1o1bcz	331	10	realtime	realtime	NN
cord-009318-zt1o1bcz	331	11	parameters	parameter	NNS
cord-009318-zt1o1bcz	331	12	,	,	,
cord-009318-zt1o1bcz	331	13	in	in	IN
cord-009318-zt1o1bcz	331	14	conjunction	conjunction	NN
cord-009318-zt1o1bcz	331	15	with	with	IN
cord-009318-zt1o1bcz	331	16	T	T	NNP
cord-009318-zt1o1bcz	331	17	m	m	NNP
cord-009318-zt1o1bcz	331	18	,	,	,
cord-009318-zt1o1bcz	331	19	could	could	MD
cord-009318-zt1o1bcz	331	20	be	be	VB
cord-009318-zt1o1bcz	331	21	used	use	VBN
cord-009318-zt1o1bcz	331	22	to	to	TO
cord-009318-zt1o1bcz	331	23	improve	improve	VB
cord-009318-zt1o1bcz	331	24	the	the	DT
cord-009318-zt1o1bcz	331	25	performance	performance	NN
cord-009318-zt1o1bcz	331	26	(	(	-LRB-
cord-009318-zt1o1bcz	331	27	LOD	LOD	NNP
cord-009318-zt1o1bcz	331	28	)	)	-RRB-
cord-009318-zt1o1bcz	331	29	of	of	IN
cord-009318-zt1o1bcz	331	30	a	a	DT
cord-009318-zt1o1bcz	331	31	dLAMP	dlamp	NN
cord-009318-zt1o1bcz	331	32	assay	assay	NN
cord-009318-zt1o1bcz	331	33	.	.	.
cord-009318-zt1o1bcz	332	1	For	for	IN
cord-009318-zt1o1bcz	332	2	any	any	DT
cord-009318-zt1o1bcz	332	3	given	give	VBN
cord-009318-zt1o1bcz	332	4	assay	assay	NN
cord-009318-zt1o1bcz	332	5	,	,	,
cord-009318-zt1o1bcz	332	6	there	there	EX
cord-009318-zt1o1bcz	332	7	is	be	VBZ
cord-009318-zt1o1bcz	332	8	a	a	DT
cord-009318-zt1o1bcz	332	9	large	large	JJ
cord-009318-zt1o1bcz	332	10	combination	combination	NN
cord-009318-zt1o1bcz	332	11	of	of	IN
cord-009318-zt1o1bcz	332	12	possible	possible	JJ
cord-009318-zt1o1bcz	332	13	parameters	parameter	NNS
cord-009318-zt1o1bcz	332	14	(	(	-LRB-
cord-009318-zt1o1bcz	332	15	e.g.	e.g.	IN
cord-009318-zt1o1bcz	332	16	amplification	amplification	NN
cord-009318-zt1o1bcz	332	17	rate	rate	NN
cord-009318-zt1o1bcz	332	18	,	,	,
cord-009318-zt1o1bcz	332	19	TTP	ttp	NN
cord-009318-zt1o1bcz	332	20	,	,	,
cord-009318-zt1o1bcz	332	21	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	332	22	intensity	intensity	NN
cord-009318-zt1o1bcz	332	23	)	)	-RRB-
cord-009318-zt1o1bcz	332	24	that	that	WDT
cord-009318-zt1o1bcz	332	25	are	be	VBP
cord-009318-zt1o1bcz	332	26	used	use	VBN
cord-009318-zt1o1bcz	332	27	to	to	TO
cord-009318-zt1o1bcz	332	28	determine	determine	VB
cord-009318-zt1o1bcz	332	29	when	when	WRB
cord-009318-zt1o1bcz	332	30	a	a	DT
cord-009318-zt1o1bcz	332	31	digital	digital	JJ
cord-009318-zt1o1bcz	332	32	partition	partition	NN
cord-009318-zt1o1bcz	332	33	is	be	VBZ
cord-009318-zt1o1bcz	332	34	'	'	``
cord-009318-zt1o1bcz	332	35	on	on	IN
cord-009318-zt1o1bcz	332	36	'	'	''
cord-009318-zt1o1bcz	332	37	or	or	CC
cord-009318-zt1o1bcz	332	38	'	'	''
cord-009318-zt1o1bcz	332	39	off	off	RB
cord-009318-zt1o1bcz	332	40	.	.	.
cord-009318-zt1o1bcz	332	41	'	'	''
cord-009318-zt1o1bcz	333	1	Use	use	NN
cord-009318-zt1o1bcz	333	2	of	of	IN
cord-009318-zt1o1bcz	333	3	these	these	DT
cord-009318-zt1o1bcz	333	4	parameters	parameter	NNS
cord-009318-zt1o1bcz	333	5	and	and	CC
cord-009318-zt1o1bcz	333	6	selection	selection	NN
cord-009318-zt1o1bcz	333	7	of	of	IN
cord-009318-zt1o1bcz	333	8	thresholds	threshold	NNS
cord-009318-zt1o1bcz	333	9	will	will	MD
cord-009318-zt1o1bcz	333	10	influence	influence	VB
cord-009318-zt1o1bcz	333	11	assay	assay	NN
cord-009318-zt1o1bcz	333	12	performance	performance	NN
cord-009318-zt1o1bcz	333	13	(	(	-LRB-
cord-009318-zt1o1bcz	333	14	analytical	analytical	JJ
cord-009318-zt1o1bcz	333	15	specificity	specificity	NN
cord-009318-zt1o1bcz	333	16	and	and	CC
cord-009318-zt1o1bcz	333	17	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	333	18	)	)	-RRB-
cord-009318-zt1o1bcz	333	19	.	.	.
cord-009318-zt1o1bcz	334	1	Assay	assay	NN
cord-009318-zt1o1bcz	334	2	performance	performance	NN
cord-009318-zt1o1bcz	334	3	is	be	VBZ
cord-009318-zt1o1bcz	334	4	affected	affect	VBN
cord-009318-zt1o1bcz	334	5	by	by	IN
cord-009318-zt1o1bcz	334	6	amplification	amplification	NN
cord-009318-zt1o1bcz	334	7	time	time	NN
cord-009318-zt1o1bcz	334	8	and	and	CC
cord-009318-zt1o1bcz	334	9	the	the	DT
cord-009318-zt1o1bcz	334	10	combination	combination	NN
cord-009318-zt1o1bcz	334	11	of	of	IN
cord-009318-zt1o1bcz	334	12	choices	choice	NNS
cord-009318-zt1o1bcz	334	13	of	of	IN
cord-009318-zt1o1bcz	334	14	parameters	parameter	NNS
cord-009318-zt1o1bcz	334	15	used	use	VBN
cord-009318-zt1o1bcz	334	16	to	to	TO
cord-009318-zt1o1bcz	334	17	process	process	VB
cord-009318-zt1o1bcz	334	18	the	the	DT
cord-009318-zt1o1bcz	334	19	data	datum	NNS
cord-009318-zt1o1bcz	334	20	impacting	impact	VBG
cord-009318-zt1o1bcz	334	21	LOD	lod	NN
cord-009318-zt1o1bcz	334	22	,	,	,
cord-009318-zt1o1bcz	334	23	the	the	DT
cord-009318-zt1o1bcz	334	24	probability	probability	NN
cord-009318-zt1o1bcz	334	25	of	of	IN
cord-009318-zt1o1bcz	334	26	detecting	detect	VBG
cord-009318-zt1o1bcz	334	27	a	a	DT
cord-009318-zt1o1bcz	334	28	molecule	molecule	NN
cord-009318-zt1o1bcz	334	29	(	(	-LRB-
cord-009318-zt1o1bcz	334	30	efficiency	efficiency	NN
cord-009318-zt1o1bcz	334	31	)	)	-RRB-
cord-009318-zt1o1bcz	334	32	,	,	,
cord-009318-zt1o1bcz	334	33	and	and	CC
cord-009318-zt1o1bcz	334	34	the	the	DT
cord-009318-zt1o1bcz	334	35	clinical	clinical	JJ
cord-009318-zt1o1bcz	334	36	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	334	37	and	and	CC
cord-009318-zt1o1bcz	334	38	specificity	specificity	NN
cord-009318-zt1o1bcz	334	39	.	.	.
cord-009318-zt1o1bcz	335	1	Having	have	VBG
cord-009318-zt1o1bcz	335	2	established	establish	VBN
cord-009318-zt1o1bcz	335	3	that	that	IN
cord-009318-zt1o1bcz	335	4	there	there	EX
cord-009318-zt1o1bcz	335	5	is	be	VBZ
cord-009318-zt1o1bcz	335	6	a	a	DT
cord-009318-zt1o1bcz	335	7	direct	direct	JJ
cord-009318-zt1o1bcz	335	8	relationship	relationship	NN
cord-009318-zt1o1bcz	335	9	between	between	IN
cord-009318-zt1o1bcz	335	10	T	T	NNP
cord-009318-zt1o1bcz	335	11	m	m	NNP
cord-009318-zt1o1bcz	335	12	,	,	,
cord-009318-zt1o1bcz	335	13	sequence	sequence	NN
cord-009318-zt1o1bcz	335	14	identity	identity	NN
cord-009318-zt1o1bcz	335	15	and	and	CC
cord-009318-zt1o1bcz	335	16	structure	structure	NN
cord-009318-zt1o1bcz	335	17	,	,	,
cord-009318-zt1o1bcz	335	18	we	-PRON-	PRP
cord-009318-zt1o1bcz	335	19	determined	determine	VBD
cord-009318-zt1o1bcz	335	20	that	that	IN
cord-009318-zt1o1bcz	335	21	T	T	NNP
cord-009318-zt1o1bcz	335	22	m	m	NN
cord-009318-zt1o1bcz	335	23	allows	allow	VBZ
cord-009318-zt1o1bcz	335	24	us	-PRON-	PRP
cord-009318-zt1o1bcz	335	25	to	to	TO
cord-009318-zt1o1bcz	335	26	explicitly	explicitly	RB
cord-009318-zt1o1bcz	335	27	differentiate	differentiate	VB
cord-009318-zt1o1bcz	335	28	specific	specific	JJ
cord-009318-zt1o1bcz	335	29	and	and	CC
cord-009318-zt1o1bcz	335	30	non	non	JJ
cord-009318-zt1o1bcz	335	31	-	-	JJ
cord-009318-zt1o1bcz	335	32	specific	specific	JJ
cord-009318-zt1o1bcz	335	33	amplification	amplification	NN
cord-009318-zt1o1bcz	335	34	in	in	IN
cord-009318-zt1o1bcz	335	35	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	335	36	,	,	,
cord-009318-zt1o1bcz	335	37	and	and	CC
cord-009318-zt1o1bcz	335	38	thus	thus	RB
cord-009318-zt1o1bcz	335	39	,	,	,
cord-009318-zt1o1bcz	335	40	differentiate	differentiate	VB
cord-009318-zt1o1bcz	335	41	true	true	JJ
cord-009318-zt1o1bcz	335	42	from	from	IN
cord-009318-zt1o1bcz	335	43	false	false	JJ
cord-009318-zt1o1bcz	335	44	positives	positive	NNS
cord-009318-zt1o1bcz	335	45	.	.	.
cord-009318-zt1o1bcz	336	1	Nucleic	Nucleic	NNP
cord-009318-zt1o1bcz	336	2	Acids	Acids	NNPS
cord-009318-zt1o1bcz	336	3	Research	Research	NNP
cord-009318-zt1o1bcz	336	4	,	,	,
cord-009318-zt1o1bcz	336	5	2020	2020	CD
cord-009318-zt1o1bcz	336	6	,	,	,
cord-009318-zt1o1bcz	336	7	Vol	Vol	NNP
cord-009318-zt1o1bcz	336	8	.	.	.
cord-009318-zt1o1bcz	337	1	48	48	CD
cord-009318-zt1o1bcz	337	2	,	,	,
cord-009318-zt1o1bcz	337	3	No	no	NN
cord-009318-zt1o1bcz	337	4	.	.	NN
cord-009318-zt1o1bcz	337	5	7	7	CD
cord-009318-zt1o1bcz	337	6	e42	e42	CD
cord-009318-zt1o1bcz	338	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	338	2	foresee	foresee	VBP
cord-009318-zt1o1bcz	338	3	two	two	CD
cord-009318-zt1o1bcz	338	4	separate	separate	JJ
cord-009318-zt1o1bcz	338	5	situations	situation	NNS
cord-009318-zt1o1bcz	338	6	of	of	IN
cord-009318-zt1o1bcz	338	7	dLAMP	dlamp	NN
cord-009318-zt1o1bcz	338	8	analysis	analysis	NN
cord-009318-zt1o1bcz	338	9	using	use	VBG
cord-009318-zt1o1bcz	338	10	HRM	HRM	NNP
cord-009318-zt1o1bcz	338	11	.	.	.
cord-009318-zt1o1bcz	339	1	First	first	RB
cord-009318-zt1o1bcz	339	2	,	,	,
cord-009318-zt1o1bcz	339	3	where	where	WRB
cord-009318-zt1o1bcz	339	4	HRM	HRM	NNP
cord-009318-zt1o1bcz	339	5	is	be	VBZ
cord-009318-zt1o1bcz	339	6	not	not	RB
cord-009318-zt1o1bcz	339	7	incorporated	incorporate	VBN
cord-009318-zt1o1bcz	339	8	in	in	IN
cord-009318-zt1o1bcz	339	9	the	the	DT
cord-009318-zt1o1bcz	339	10	final	final	JJ
cord-009318-zt1o1bcz	339	11	assay	assay	NN
cord-009318-zt1o1bcz	339	12	,	,	,
cord-009318-zt1o1bcz	339	13	but	but	CC
cord-009318-zt1o1bcz	339	14	is	be	VBZ
cord-009318-zt1o1bcz	339	15	used	use	VBN
cord-009318-zt1o1bcz	339	16	during	during	IN
cord-009318-zt1o1bcz	339	17	assay	assay	NN
cord-009318-zt1o1bcz	339	18	development	development	NN
cord-009318-zt1o1bcz	339	19	.	.	.
cord-009318-zt1o1bcz	340	1	Second	second	RB
cord-009318-zt1o1bcz	340	2	,	,	,
cord-009318-zt1o1bcz	340	3	the	the	DT
cord-009318-zt1o1bcz	340	4	ideal	ideal	JJ
cord-009318-zt1o1bcz	340	5	situation	situation	NN
cord-009318-zt1o1bcz	340	6	for	for	IN
cord-009318-zt1o1bcz	340	7	quantitative	quantitative	JJ
cord-009318-zt1o1bcz	340	8	performance	performance	NN
cord-009318-zt1o1bcz	340	9	,	,	,
cord-009318-zt1o1bcz	340	10	where	where	WRB
cord-009318-zt1o1bcz	340	11	HRM	HRM	NNP
cord-009318-zt1o1bcz	340	12	is	be	VBZ
cord-009318-zt1o1bcz	340	13	incorporated	incorporate	VBN
cord-009318-zt1o1bcz	340	14	into	into	IN
cord-009318-zt1o1bcz	340	15	the	the	DT
cord-009318-zt1o1bcz	340	16	final	final	JJ
cord-009318-zt1o1bcz	340	17	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	340	18	assay	assay	NN
cord-009318-zt1o1bcz	340	19	.	.	.
cord-009318-zt1o1bcz	341	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	341	2	expect	expect	VBP
cord-009318-zt1o1bcz	341	3	the	the	DT
cord-009318-zt1o1bcz	341	4	first	first	JJ
cord-009318-zt1o1bcz	341	5	group	group	NN
cord-009318-zt1o1bcz	341	6	of	of	IN
cord-009318-zt1o1bcz	341	7	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	341	8	assays	assay	NNS
cord-009318-zt1o1bcz	341	9	to	to	TO
cord-009318-zt1o1bcz	341	10	exist	exist	VB
cord-009318-zt1o1bcz	341	11	because	because	IN
cord-009318-zt1o1bcz	341	12	collecting	collect	VBG
cord-009318-zt1o1bcz	341	13	T	T	NNP
cord-009318-zt1o1bcz	341	14	m	m	NNP
cord-009318-zt1o1bcz	341	15	data	datum	NNS
cord-009318-zt1o1bcz	341	16	adds	add	VBZ
cord-009318-zt1o1bcz	341	17	additional	additional	JJ
cord-009318-zt1o1bcz	341	18	time	time	NN
cord-009318-zt1o1bcz	341	19	to	to	IN
cord-009318-zt1o1bcz	341	20	an	an	DT
cord-009318-zt1o1bcz	341	21	assay	assay	NN
cord-009318-zt1o1bcz	341	22	and	and	CC
cord-009318-zt1o1bcz	341	23	requires	require	VBZ
cord-009318-zt1o1bcz	341	24	more	more	RBR
cord-009318-zt1o1bcz	341	25	advanced	advanced	JJ
cord-009318-zt1o1bcz	341	26	hardware	hardware	NN
cord-009318-zt1o1bcz	341	27	to	to	TO
cord-009318-zt1o1bcz	341	28	run	run	VB
cord-009318-zt1o1bcz	341	29	.	.	.
cord-009318-zt1o1bcz	342	1	This	this	DT
cord-009318-zt1o1bcz	342	2	may	may	MD
cord-009318-zt1o1bcz	342	3	be	be	VB
cord-009318-zt1o1bcz	342	4	unideal	unideal	JJ
cord-009318-zt1o1bcz	342	5	in	in	IN
cord-009318-zt1o1bcz	342	6	situations	situation	NNS
cord-009318-zt1o1bcz	342	7	requiring	require	VBG
cord-009318-zt1o1bcz	342	8	more	more	RBR
cord-009318-zt1o1bcz	342	9	rapid	rapid	JJ
cord-009318-zt1o1bcz	342	10	diagnostics	diagnostic	NNS
cord-009318-zt1o1bcz	342	11	or	or	CC
cord-009318-zt1o1bcz	342	12	limited	limited	JJ
cord-009318-zt1o1bcz	342	13	-	-	HYPH
cord-009318-zt1o1bcz	342	14	resource	resource	NN
cord-009318-zt1o1bcz	342	15	and	and	CC
cord-009318-zt1o1bcz	342	16	field	field	NN
cord-009318-zt1o1bcz	342	17	settings	setting	NNS
cord-009318-zt1o1bcz	342	18	where	where	WRB
cord-009318-zt1o1bcz	342	19	the	the	DT
cord-009318-zt1o1bcz	342	20	hardware	hardware	NN
cord-009318-zt1o1bcz	342	21	may	may	MD
cord-009318-zt1o1bcz	342	22	be	be	VB
cord-009318-zt1o1bcz	342	23	impractical	impractical	JJ
cord-009318-zt1o1bcz	342	24	.	.	.
cord-009318-zt1o1bcz	343	1	Nonetheless	nonetheless	RB
cord-009318-zt1o1bcz	343	2	,	,	,
cord-009318-zt1o1bcz	343	3	running	run	VBG
cord-009318-zt1o1bcz	343	4	HRM	HRM	NNP
cord-009318-zt1o1bcz	343	5	is	be	VBZ
cord-009318-zt1o1bcz	343	6	still	still	RB
cord-009318-zt1o1bcz	343	7	useful	useful	JJ
cord-009318-zt1o1bcz	343	8	during	during	IN
cord-009318-zt1o1bcz	343	9	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	343	10	assay	assay	NN
cord-009318-zt1o1bcz	343	11	development	development	NN
cord-009318-zt1o1bcz	343	12	to	to	TO
cord-009318-zt1o1bcz	343	13	select	select	VB
cord-009318-zt1o1bcz	343	14	the	the	DT
cord-009318-zt1o1bcz	343	15	optimal	optimal	JJ
cord-009318-zt1o1bcz	343	16	combination	combination	NN
cord-009318-zt1o1bcz	343	17	of	of	IN
cord-009318-zt1o1bcz	343	18	parameters	parameter	NNS
cord-009318-zt1o1bcz	343	19	for	for	IN
cord-009318-zt1o1bcz	343	20	end	end	NN
cord-009318-zt1o1bcz	343	21	-	-	HYPH
cord-009318-zt1o1bcz	343	22	point	point	NN
cord-009318-zt1o1bcz	343	23	or	or	CC
cord-009318-zt1o1bcz	343	24	real	real	JJ
cord-009318-zt1o1bcz	343	25	-	-	HYPH
cord-009318-zt1o1bcz	343	26	time	time	NN
cord-009318-zt1o1bcz	343	27	LAMP	lamp	NN
cord-009318-zt1o1bcz	343	28	without	without	IN
cord-009318-zt1o1bcz	343	29	using	use	VBG
cord-009318-zt1o1bcz	343	30	T	T	NNP
cord-009318-zt1o1bcz	343	31	m	m	NNP
cord-009318-zt1o1bcz	343	32	.	.	.
cord-009318-zt1o1bcz	344	1	Hence	hence	RB
cord-009318-zt1o1bcz	344	2	,	,	,
cord-009318-zt1o1bcz	344	3	T	T	NNP
cord-009318-zt1o1bcz	344	4	m	m	NN
cord-009318-zt1o1bcz	344	5	allows	allow	VBZ
cord-009318-zt1o1bcz	344	6	one	one	PRP
cord-009318-zt1o1bcz	344	7	to	to	TO
cord-009318-zt1o1bcz	344	8	identify	identify	VB
cord-009318-zt1o1bcz	344	9	the	the	DT
cord-009318-zt1o1bcz	344	10	correct	correct	JJ
cord-009318-zt1o1bcz	344	11	combination	combination	NN
cord-009318-zt1o1bcz	344	12	of	of	IN
cord-009318-zt1o1bcz	344	13	assay	assay	NN
cord-009318-zt1o1bcz	344	14	parameters	parameter	NNS
cord-009318-zt1o1bcz	344	15	,	,	,
cord-009318-zt1o1bcz	344	16	and	and	CC
cord-009318-zt1o1bcz	344	17	how	how	WRB
cord-009318-zt1o1bcz	344	18	to	to	TO
cord-009318-zt1o1bcz	344	19	analyze	analyze	VB
cord-009318-zt1o1bcz	344	20	the	the	DT
cord-009318-zt1o1bcz	344	21	data	datum	NNS
cord-009318-zt1o1bcz	344	22	for	for	IN
cord-009318-zt1o1bcz	344	23	best	good	JJS
cord-009318-zt1o1bcz	344	24	LOD	lod	NN
cord-009318-zt1o1bcz	344	25	.	.	.
cord-009318-zt1o1bcz	345	1	LOD	LOD	NNP
cord-009318-zt1o1bcz	345	2	is	be	VBZ
cord-009318-zt1o1bcz	345	3	a	a	DT
cord-009318-zt1o1bcz	345	4	key	key	JJ
cord-009318-zt1o1bcz	345	5	parameter	parameter	NN
cord-009318-zt1o1bcz	345	6	when	when	WRB
cord-009318-zt1o1bcz	345	7	optimizing	optimize	VBG
cord-009318-zt1o1bcz	345	8	clinical	clinical	JJ
cord-009318-zt1o1bcz	345	9	assays	assay	NNS
cord-009318-zt1o1bcz	345	10	because	because	IN
cord-009318-zt1o1bcz	345	11	pathogen	pathogen	NN
cord-009318-zt1o1bcz	345	12	load	load	NN
cord-009318-zt1o1bcz	345	13	is	be	VBZ
cord-009318-zt1o1bcz	345	14	low	low	JJ
cord-009318-zt1o1bcz	345	15	in	in	IN
cord-009318-zt1o1bcz	345	16	many	many	JJ
cord-009318-zt1o1bcz	345	17	infections	infection	NNS
cord-009318-zt1o1bcz	345	18	(	(	-LRB-
cord-009318-zt1o1bcz	345	19	e.g.	e.g.	RB
cord-009318-zt1o1bcz	345	20	in	in	IN
cord-009318-zt1o1bcz	345	21	blood	blood	NN
cord-009318-zt1o1bcz	345	22	infections	infection	NNS
cord-009318-zt1o1bcz	345	23	or	or	CC
cord-009318-zt1o1bcz	345	24	asymptomatic	asymptomatic	JJ
cord-009318-zt1o1bcz	345	25	sexually	sexually	RB
cord-009318-zt1o1bcz	345	26	transmitted	transmit	VBN
cord-009318-zt1o1bcz	345	27	infections	infection	NNS
cord-009318-zt1o1bcz	345	28	)	)	-RRB-
cord-009318-zt1o1bcz	345	29	.	.	.
cord-009318-zt1o1bcz	346	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	346	2	thus	thus	RB
cord-009318-zt1o1bcz	346	3	illustrated	illustrate	VBD
cord-009318-zt1o1bcz	346	4	the	the	DT
cord-009318-zt1o1bcz	346	5	optimization	optimization	NN
cord-009318-zt1o1bcz	346	6	of	of	IN
cord-009318-zt1o1bcz	346	7	parameters	parameter	NNS
cord-009318-zt1o1bcz	346	8	using	use	VBG
cord-009318-zt1o1bcz	346	9	improved	improve	VBN
cord-009318-zt1o1bcz	346	10	LOD	lod	NN
cord-009318-zt1o1bcz	346	11	as	as	IN
cord-009318-zt1o1bcz	346	12	the	the	DT
cord-009318-zt1o1bcz	346	13	selection	selection	NN
cord-009318-zt1o1bcz	346	14	criteria	criterion	NNS
cord-009318-zt1o1bcz	346	15	.	.	.
cord-009318-zt1o1bcz	347	1	The	the	DT
cord-009318-zt1o1bcz	347	2	combination	combination	NN
cord-009318-zt1o1bcz	347	3	of	of	IN
cord-009318-zt1o1bcz	347	4	real	real	JJ
cord-009318-zt1o1bcz	347	5	-	-	HYPH
cord-009318-zt1o1bcz	347	6	time	time	NN
cord-009318-zt1o1bcz	347	7	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	347	8	with	with	IN
cord-009318-zt1o1bcz	347	9	HRM	HRM	NNP
cord-009318-zt1o1bcz	347	10	can	can	MD
cord-009318-zt1o1bcz	347	11	uniquely	uniquely	RB
cord-009318-zt1o1bcz	347	12	define	define	VB
cord-009318-zt1o1bcz	347	13	LOD	lod	NN
cord-009318-zt1o1bcz	347	14	because	because	IN
cord-009318-zt1o1bcz	347	15	of	of	IN
cord-009318-zt1o1bcz	347	16	the	the	DT
cord-009318-zt1o1bcz	347	17	combination	combination	NN
cord-009318-zt1o1bcz	347	18	of	of	IN
cord-009318-zt1o1bcz	347	19	digital	digital	NNP
cord-009318-zt1o1bcz	347	20	and	and	CC
cord-009318-zt1o1bcz	347	21	T	T	NNP
cord-009318-zt1o1bcz	347	22	m	m	NN
cord-009318-zt1o1bcz	347	23	.	.	.
cord-009318-zt1o1bcz	348	1	Unlike	unlike	IN
cord-009318-zt1o1bcz	348	2	bulk	bulk	JJ
cord-009318-zt1o1bcz	348	3	assays	assay	NNS
cord-009318-zt1o1bcz	348	4	,	,	,
cord-009318-zt1o1bcz	348	5	which	which	WDT
cord-009318-zt1o1bcz	348	6	require	require	VBP
cord-009318-zt1o1bcz	348	7	a	a	DT
cord-009318-zt1o1bcz	348	8	concentration	concentration	NN
cord-009318-zt1o1bcz	348	9	titration	titration	NN
cord-009318-zt1o1bcz	348	10	curve	curve	NN
cord-009318-zt1o1bcz	348	11	(	(	-LRB-
cord-009318-zt1o1bcz	348	12	and	and	CC
cord-009318-zt1o1bcz	348	13	are	be	VBP
cord-009318-zt1o1bcz	348	14	thus	thus	RB
cord-009318-zt1o1bcz	348	15	dependent	dependent	JJ
cord-009318-zt1o1bcz	348	16	on	on	IN
cord-009318-zt1o1bcz	348	17	integrated	integrate	VBN
cord-009318-zt1o1bcz	348	18	signal	signal	JJ
cord-009318-zt1o1bcz	348	19	intensity	intensity	NN
cord-009318-zt1o1bcz	348	20	and	and	CC
cord-009318-zt1o1bcz	348	21	enzymatic	enzymatic	JJ
cord-009318-zt1o1bcz	348	22	turnover	turnover	NN
cord-009318-zt1o1bcz	348	23	)	)	-RRB-
cord-009318-zt1o1bcz	348	24	,	,	,
cord-009318-zt1o1bcz	348	25	digital	digital	JJ
cord-009318-zt1o1bcz	348	26	assays	assay	NNS
cord-009318-zt1o1bcz	348	27	only	only	RB
cord-009318-zt1o1bcz	348	28	require	require	VBP
cord-009318-zt1o1bcz	348	29	that	that	IN
cord-009318-zt1o1bcz	348	30	an	an	DT
cord-009318-zt1o1bcz	348	31	event	event	NN
cord-009318-zt1o1bcz	348	32	(	(	-LRB-
cord-009318-zt1o1bcz	348	33	target	target	NN
cord-009318-zt1o1bcz	348	34	molecule	molecule	NN
cord-009318-zt1o1bcz	348	35	)	)	-RRB-
cord-009318-zt1o1bcz	348	36	is	be	VBZ
cord-009318-zt1o1bcz	348	37	or	or	CC
cord-009318-zt1o1bcz	348	38	is	be	VBZ
cord-009318-zt1o1bcz	348	39	not	not	RB
cord-009318-zt1o1bcz	348	40	observed	observe	VBN
cord-009318-zt1o1bcz	348	41	and	and	CC
cord-009318-zt1o1bcz	348	42	can	can	MD
cord-009318-zt1o1bcz	348	43	be	be	VB
cord-009318-zt1o1bcz	348	44	counted	count	VBN
cord-009318-zt1o1bcz	348	45	relative	relative	JJ
cord-009318-zt1o1bcz	348	46	to	to	IN
cord-009318-zt1o1bcz	348	47	the	the	DT
cord-009318-zt1o1bcz	348	48	partition	partition	NN
cord-009318-zt1o1bcz	348	49	volume	volume	NN
cord-009318-zt1o1bcz	348	50	(	(	-LRB-
cord-009318-zt1o1bcz	348	51	40	40	CD
cord-009318-zt1o1bcz	348	52	,	,	,
cord-009318-zt1o1bcz	348	53	41	41	CD
cord-009318-zt1o1bcz	348	54	)	)	-RRB-
cord-009318-zt1o1bcz	348	55	.	.	.
cord-009318-zt1o1bcz	349	1	The	the	DT
cord-009318-zt1o1bcz	349	2	minimum	minimum	NN
cord-009318-zt1o1bcz	349	3	LOD	lod	NN
cord-009318-zt1o1bcz	349	4	for	for	IN
cord-009318-zt1o1bcz	349	5	any	any	DT
cord-009318-zt1o1bcz	349	6	digital	digital	JJ
cord-009318-zt1o1bcz	349	7	assay	assay	NN
cord-009318-zt1o1bcz	349	8	corresponds	correspond	VBZ
cord-009318-zt1o1bcz	349	9	to	to	IN
cord-009318-zt1o1bcz	349	10	one	one	CD
cord-009318-zt1o1bcz	349	11	target	target	NN
cord-009318-zt1o1bcz	349	12	or	or	CC
cord-009318-zt1o1bcz	349	13	amplification	amplification	NN
cord-009318-zt1o1bcz	349	14	event	event	NN
cord-009318-zt1o1bcz	349	15	per	per	IN
cord-009318-zt1o1bcz	349	16	partition	partition	NN
cord-009318-zt1o1bcz	349	17	volume	volume	NN
cord-009318-zt1o1bcz	349	18	.	.	.
cord-009318-zt1o1bcz	350	1	Hence	hence	RB
cord-009318-zt1o1bcz	350	2	,	,	,
cord-009318-zt1o1bcz	350	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	350	4	can	can	MD
cord-009318-zt1o1bcz	350	5	define	define	VB
cord-009318-zt1o1bcz	350	6	LOD	lod	NN
cord-009318-zt1o1bcz	350	7	from	from	IN
cord-009318-zt1o1bcz	350	8	a	a	DT
cord-009318-zt1o1bcz	350	9	single	single	JJ
cord-009318-zt1o1bcz	350	10	concentration	concentration	NN
cord-009318-zt1o1bcz	350	11	point	point	NN
cord-009318-zt1o1bcz	350	12	by	by	IN
cord-009318-zt1o1bcz	350	13	Equation	equation	NN
cord-009318-zt1o1bcz	350	14	(	(	-LRB-
cord-009318-zt1o1bcz	350	15	1	1	CD
cord-009318-zt1o1bcz	350	16	)	)	-RRB-
cord-009318-zt1o1bcz	350	17	:	:	:
cord-009318-zt1o1bcz	350	18	where	where	WRB
cord-009318-zt1o1bcz	350	19	C	C	NNP
cord-009318-zt1o1bcz	350	20	True	true	JJ
cord-009318-zt1o1bcz	350	21	is	be	VBZ
cord-009318-zt1o1bcz	350	22	the	the	DT
cord-009318-zt1o1bcz	350	23	concentration	concentration	NN
cord-009318-zt1o1bcz	350	24	of	of	IN
cord-009318-zt1o1bcz	350	25	target	target	NN
cord-009318-zt1o1bcz	350	26	molecules	molecule	NNS
cord-009318-zt1o1bcz	350	27	loaded	load	VBN
cord-009318-zt1o1bcz	350	28	by	by	IN
cord-009318-zt1o1bcz	350	29	ddPCR	ddPCR	NNP
cord-009318-zt1o1bcz	350	30	counts	count	NNS
cord-009318-zt1o1bcz	350	31	in	in	IN
cord-009318-zt1o1bcz	350	32	copies	copy	NNS
cord-009318-zt1o1bcz	350	33	per	per	IN
cord-009318-zt1o1bcz	350	34	microliter	microliter	NN
cord-009318-zt1o1bcz	350	35	,	,	.
cord-009318-zt1o1bcz	351	1	N	N	NNP
cord-009318-zt1o1bcz	351	2	True	true	JJ
cord-009318-zt1o1bcz	351	3	is	be	VBZ
cord-009318-zt1o1bcz	351	4	the	the	DT
cord-009318-zt1o1bcz	351	5	number	number	NN
cord-009318-zt1o1bcz	351	6	of	of	IN
cord-009318-zt1o1bcz	351	7	true	true	JJ
cord-009318-zt1o1bcz	351	8	positive	positive	JJ
cord-009318-zt1o1bcz	351	9	(	(	-LRB-
cord-009318-zt1o1bcz	351	10	specific	specific	JJ
cord-009318-zt1o1bcz	351	11	)	)	-RRB-
cord-009318-zt1o1bcz	351	12	amplification	amplification	NN
cord-009318-zt1o1bcz	351	13	events	event	NNS
cord-009318-zt1o1bcz	351	14	observed	observe	VBN
cord-009318-zt1o1bcz	351	15	on	on	IN
cord-009318-zt1o1bcz	351	16	a	a	DT
cord-009318-zt1o1bcz	351	17	chip	chip	NN
cord-009318-zt1o1bcz	351	18	,	,	,
cord-009318-zt1o1bcz	351	19	N	N	NNP
cord-009318-zt1o1bcz	351	20	False	False	NNP
cord-009318-zt1o1bcz	351	21	is	be	VBZ
cord-009318-zt1o1bcz	351	22	the	the	DT
cord-009318-zt1o1bcz	351	23	number	number	NN
cord-009318-zt1o1bcz	351	24	of	of	IN
cord-009318-zt1o1bcz	351	25	non	non	JJ
cord-009318-zt1o1bcz	351	26	-	-	JJ
cord-009318-zt1o1bcz	351	27	specific	specific	JJ
cord-009318-zt1o1bcz	351	28	amplification	amplification	NN
cord-009318-zt1o1bcz	351	29	events	event	NNS
cord-009318-zt1o1bcz	351	30	observed	observe	VBN
cord-009318-zt1o1bcz	351	31	on	on	IN
cord-009318-zt1o1bcz	351	32	a	a	DT
cord-009318-zt1o1bcz	351	33	chip	chip	NN
cord-009318-zt1o1bcz	351	34	and	and	CC
cord-009318-zt1o1bcz	351	35	N	N	NNP
cord-009318-zt1o1bcz	351	36	CI	CI	NNP
cord-009318-zt1o1bcz	352	1	is	be	VBZ
cord-009318-zt1o1bcz	352	2	the	the	DT
cord-009318-zt1o1bcz	352	3	number	number	NN
cord-009318-zt1o1bcz	352	4	of	of	IN
cord-009318-zt1o1bcz	352	5	expected	expect	VBN
cord-009318-zt1o1bcz	352	6	molecules	molecule	NNS
cord-009318-zt1o1bcz	352	7	for	for	IN
cord-009318-zt1o1bcz	352	8	a	a	DT
cord-009318-zt1o1bcz	352	9	given	give	VBN
cord-009318-zt1o1bcz	352	10	confidence	confidence	NN
cord-009318-zt1o1bcz	352	11	interval	interval	NN
cord-009318-zt1o1bcz	352	12	.	.	.
cord-009318-zt1o1bcz	353	1	In	in	IN
cord-009318-zt1o1bcz	353	2	this	this	DT
cord-009318-zt1o1bcz	353	3	equation	equation	NN
cord-009318-zt1o1bcz	353	4	,	,	,
cord-009318-zt1o1bcz	353	5	the	the	DT
cord-009318-zt1o1bcz	353	6	N	N	NNP
cord-009318-zt1o1bcz	353	7	True	True	NNP
cord-009318-zt1o1bcz	353	8	and	and	CC
cord-009318-zt1o1bcz	353	9	N	n	CD
cord-009318-zt1o1bcz	353	10	False	false	NN
cord-009318-zt1o1bcz	353	11	are	be	VBP
cord-009318-zt1o1bcz	353	12	chip	chip	NN
cord-009318-zt1o1bcz	353	13	-	-	HYPH
cord-009318-zt1o1bcz	353	14	specific	specific	JJ
cord-009318-zt1o1bcz	353	15	,	,	,
cord-009318-zt1o1bcz	353	16	and	and	CC
cord-009318-zt1o1bcz	353	17	take	take	VB
cord-009318-zt1o1bcz	353	18	into	into	IN
cord-009318-zt1o1bcz	353	19	account	account	NN
cord-009318-zt1o1bcz	353	20	the	the	DT
cord-009318-zt1o1bcz	353	21	total	total	JJ
cord-009318-zt1o1bcz	353	22	volume	volume	NN
cord-009318-zt1o1bcz	353	23	of	of	IN
cord-009318-zt1o1bcz	353	24	the	the	DT
cord-009318-zt1o1bcz	353	25	chip	chip	NN
cord-009318-zt1o1bcz	353	26	,	,	,
cord-009318-zt1o1bcz	353	27	the	the	DT
cord-009318-zt1o1bcz	353	28	number	number	NN
cord-009318-zt1o1bcz	353	29	of	of	IN
cord-009318-zt1o1bcz	353	30	partitions	partition	NNS
cord-009318-zt1o1bcz	353	31	and	and	CC
cord-009318-zt1o1bcz	353	32	the	the	DT
cord-009318-zt1o1bcz	353	33	volume	volume	NN
cord-009318-zt1o1bcz	353	34	of	of	IN
cord-009318-zt1o1bcz	353	35	partitions	partition	NNS
cord-009318-zt1o1bcz	353	36	.	.	.
cord-009318-zt1o1bcz	354	1	Furthermore	furthermore	RB
cord-009318-zt1o1bcz	354	2	,	,	,
cord-009318-zt1o1bcz	354	3	in	in	IN
cord-009318-zt1o1bcz	354	4	Equation	equation	NN
cord-009318-zt1o1bcz	354	5	(	(	-LRB-
cord-009318-zt1o1bcz	354	6	1	1	CD
cord-009318-zt1o1bcz	354	7	)	)	-RRB-
cord-009318-zt1o1bcz	354	8	,	,	,
cord-009318-zt1o1bcz	354	9	amplification	amplification	NN
cord-009318-zt1o1bcz	354	10	efficiency	efficiency	NN
cord-009318-zt1o1bcz	354	11	is	be	VBZ
cord-009318-zt1o1bcz	354	12	implicitly	implicitly	RB
cord-009318-zt1o1bcz	354	13	taken	take	VBN
cord-009318-zt1o1bcz	354	14	into	into	IN
cord-009318-zt1o1bcz	354	15	account	account	NN
cord-009318-zt1o1bcz	354	16	via	via	IN
cord-009318-zt1o1bcz	354	17	the	the	DT
cord-009318-zt1o1bcz	354	18	N	N	NNP
cord-009318-zt1o1bcz	354	19	True	true	JJ
cord-009318-zt1o1bcz	354	20	parameter	parameter	NN
cord-009318-zt1o1bcz	354	21	(	(	-LRB-
cord-009318-zt1o1bcz	354	22	in	in	IN
cord-009318-zt1o1bcz	354	23	other	other	JJ
cord-009318-zt1o1bcz	354	24	words	word	NNS
cord-009318-zt1o1bcz	354	25	,	,	,
cord-009318-zt1o1bcz	354	26	for	for	IN
cord-009318-zt1o1bcz	354	27	a	a	DT
cord-009318-zt1o1bcz	354	28	less	less	RBR
cord-009318-zt1o1bcz	354	29	efficient	efficient	JJ
cord-009318-zt1o1bcz	354	30	amplification	amplification	NN
cord-009318-zt1o1bcz	354	31	process	process	NN
cord-009318-zt1o1bcz	354	32	,	,	,
cord-009318-zt1o1bcz	354	33	a	a	DT
cord-009318-zt1o1bcz	354	34	given	give	VBN
cord-009318-zt1o1bcz	354	35	C	c	NN
cord-009318-zt1o1bcz	354	36	True	true	JJ
cord-009318-zt1o1bcz	354	37	on	on	IN
cord-009318-zt1o1bcz	354	38	a	a	DT
cord-009318-zt1o1bcz	354	39	given	give	VBN
cord-009318-zt1o1bcz	354	40	chip	chip	NN
cord-009318-zt1o1bcz	354	41	would	would	MD
cord-009318-zt1o1bcz	354	42	lead	lead	VB
cord-009318-zt1o1bcz	354	43	to	to	IN
cord-009318-zt1o1bcz	354	44	a	a	DT
cord-009318-zt1o1bcz	354	45	lower	low	JJR
cord-009318-zt1o1bcz	354	46	value	value	NN
cord-009318-zt1o1bcz	354	47	of	of	IN
cord-009318-zt1o1bcz	354	48	N	N	NNP
cord-009318-zt1o1bcz	354	49	True	True	NNP
cord-009318-zt1o1bcz	354	50	)	)	-RRB-
cord-009318-zt1o1bcz	354	51	.	.	.
cord-009318-zt1o1bcz	355	1	For	for	IN
cord-009318-zt1o1bcz	355	2	simplicity	simplicity	NN
cord-009318-zt1o1bcz	355	3	,	,	,
cord-009318-zt1o1bcz	355	4	Equation	equation	NN
cord-009318-zt1o1bcz	355	5	(	(	-LRB-
cord-009318-zt1o1bcz	355	6	1	1	CD
cord-009318-zt1o1bcz	355	7	)	)	-RRB-
cord-009318-zt1o1bcz	355	8	makes	make	VBZ
cord-009318-zt1o1bcz	355	9	the	the	DT
cord-009318-zt1o1bcz	355	10	assumption	assumption	NN
cord-009318-zt1o1bcz	355	11	that	that	IN
cord-009318-zt1o1bcz	355	12	the	the	DT
cord-009318-zt1o1bcz	355	13	measurements	measurement	NNS
cord-009318-zt1o1bcz	355	14	are	be	VBP
cord-009318-zt1o1bcz	355	15	performed	perform	VBN
cord-009318-zt1o1bcz	355	16	at	at	IN
cord-009318-zt1o1bcz	355	17	sufficiently	sufficiently	RB
cord-009318-zt1o1bcz	355	18	low	low	JJ
cord-009318-zt1o1bcz	355	19	concentrations	concentration	NNS
cord-009318-zt1o1bcz	355	20	(	(	-LRB-
cord-009318-zt1o1bcz	355	21	as	as	IN
cord-009318-zt1o1bcz	355	22	is	be	VBZ
cord-009318-zt1o1bcz	355	23	typical	typical	JJ
cord-009318-zt1o1bcz	355	24	for	for	IN
cord-009318-zt1o1bcz	355	25	LOD	lod	NN
cord-009318-zt1o1bcz	355	26	experiments	experiment	NNS
cord-009318-zt1o1bcz	355	27	)	)	-RRB-
cord-009318-zt1o1bcz	355	28	that	that	IN
cord-009318-zt1o1bcz	355	29	only	only	RB
cord-009318-zt1o1bcz	355	30	a	a	DT
cord-009318-zt1o1bcz	355	31	very	very	RB
cord-009318-zt1o1bcz	355	32	small	small	JJ
cord-009318-zt1o1bcz	355	33	fraction	fraction	NN
cord-009318-zt1o1bcz	355	34	of	of	IN
cord-009318-zt1o1bcz	355	35	occupied	occupy	VBN
cord-009318-zt1o1bcz	355	36	partitions	partition	NNS
cord-009318-zt1o1bcz	355	37	contain	contain	VBP
cord-009318-zt1o1bcz	355	38	more	more	JJR
cord-009318-zt1o1bcz	355	39	than	than	IN
cord-009318-zt1o1bcz	355	40	one	one	CD
cord-009318-zt1o1bcz	355	41	molecule	molecule	NN
cord-009318-zt1o1bcz	355	42	and	and	CC
cord-009318-zt1o1bcz	355	43	therefore	therefore	RB
cord-009318-zt1o1bcz	355	44	there	there	EX
cord-009318-zt1o1bcz	355	45	is	be	VBZ
cord-009318-zt1o1bcz	355	46	a	a	DT
cord-009318-zt1o1bcz	355	47	linear	linear	JJ
cord-009318-zt1o1bcz	355	48	relationship	relationship	NN
cord-009318-zt1o1bcz	355	49	between	between	IN
cord-009318-zt1o1bcz	355	50	C	C	NNP
cord-009318-zt1o1bcz	355	51	True	true	JJ
cord-009318-zt1o1bcz	355	52	and	and	CC
cord-009318-zt1o1bcz	355	53	N	N	NNP
cord-009318-zt1o1bcz	355	54	True	true	JJ
cord-009318-zt1o1bcz	355	55	.	.	.
cord-009318-zt1o1bcz	356	1	The	the	DT
cord-009318-zt1o1bcz	356	2	concentration	concentration	NN
cord-009318-zt1o1bcz	356	3	loaded	load	VBN
cord-009318-zt1o1bcz	356	4	,	,	,
cord-009318-zt1o1bcz	356	5	C	C	NNP
cord-009318-zt1o1bcz	356	6	True	True	NNP
cord-009318-zt1o1bcz	356	7	,	,	,
cord-009318-zt1o1bcz	356	8	generates	generate	VBZ
cord-009318-zt1o1bcz	356	9	N	n	CD
cord-009318-zt1o1bcz	356	10	total	total	JJ
cord-009318-zt1o1bcz	356	11	counts	count	NNS
cord-009318-zt1o1bcz	356	12	of	of	IN
cord-009318-zt1o1bcz	356	13	both	both	DT
cord-009318-zt1o1bcz	356	14	true	true	JJ
cord-009318-zt1o1bcz	356	15	-	-	HYPH
cord-009318-zt1o1bcz	356	16	and	and	CC
cord-009318-zt1o1bcz	356	17	false	false	JJ
cord-009318-zt1o1bcz	356	18	-	-	HYPH
cord-009318-zt1o1bcz	356	19	positive	positive	JJ
cord-009318-zt1o1bcz	356	20	events	event	NNS
cord-009318-zt1o1bcz	356	21	.	.	.
cord-009318-zt1o1bcz	357	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	357	2	can	can	MD
cord-009318-zt1o1bcz	357	3	divide	divide	VB
cord-009318-zt1o1bcz	357	4	this	this	DT
cord-009318-zt1o1bcz	357	5	concentration	concentration	NN
cord-009318-zt1o1bcz	357	6	by	by	IN
cord-009318-zt1o1bcz	357	7	the	the	DT
cord-009318-zt1o1bcz	357	8	minimal	minimal	JJ
cord-009318-zt1o1bcz	357	9	number	number	NN
cord-009318-zt1o1bcz	357	10	of	of	IN
cord-009318-zt1o1bcz	357	11	counts	count	NNS
cord-009318-zt1o1bcz	357	12	needed	need	VBN
cord-009318-zt1o1bcz	357	13	to	to	TO
cord-009318-zt1o1bcz	357	14	identify	identify	VB
cord-009318-zt1o1bcz	357	15	a	a	DT
cord-009318-zt1o1bcz	357	16	specific	specific	JJ
cord-009318-zt1o1bcz	357	17	amplification	amplification	NN
cord-009318-zt1o1bcz	357	18	event	event	NN
cord-009318-zt1o1bcz	357	19	and	and	CC
cord-009318-zt1o1bcz	357	20	define	define	VB
cord-009318-zt1o1bcz	357	21	this	this	DT
cord-009318-zt1o1bcz	357	22	as	as	IN
cord-009318-zt1o1bcz	357	23	the	the	DT
cord-009318-zt1o1bcz	357	24	LOD	lod	NN
cord-009318-zt1o1bcz	357	25	.	.	.
cord-009318-zt1o1bcz	358	1	The	the	DT
cord-009318-zt1o1bcz	358	2	minimum	minimum	JJ
cord-009318-zt1o1bcz	358	3	number	number	NN
cord-009318-zt1o1bcz	358	4	of	of	IN
cord-009318-zt1o1bcz	358	5	counts	count	NNS
cord-009318-zt1o1bcz	358	6	needed	need	VBN
cord-009318-zt1o1bcz	358	7	to	to	TO
cord-009318-zt1o1bcz	358	8	guarantee	guarantee	VB
cord-009318-zt1o1bcz	358	9	a	a	DT
cord-009318-zt1o1bcz	358	10	specific	specific	JJ
cord-009318-zt1o1bcz	358	11	amplification	amplification	NN
cord-009318-zt1o1bcz	358	12	event	event	NN
cord-009318-zt1o1bcz	358	13	is	be	VBZ
cord-009318-zt1o1bcz	358	14	observed	observe	VBN
cord-009318-zt1o1bcz	358	15	is	be	VBZ
cord-009318-zt1o1bcz	358	16	determined	determine	VBN
cord-009318-zt1o1bcz	358	17	by	by	IN
cord-009318-zt1o1bcz	358	18	N	N	NNP
cord-009318-zt1o1bcz	358	19	True	True	NNP
cord-009318-zt1o1bcz	358	20	,	,	,
cord-009318-zt1o1bcz	358	21	N	N	NNP
cord-009318-zt1o1bcz	358	22	False	False	NNP
cord-009318-zt1o1bcz	358	23	and	and	CC
cord-009318-zt1o1bcz	358	24	N	N	NNP
cord-009318-zt1o1bcz	358	25	CI	CI	NNP
cord-009318-zt1o1bcz	358	26	.	.	.
cord-009318-zt1o1bcz	359	1	N	N	NNP
cord-009318-zt1o1bcz	360	1	True	true	JJ
cord-009318-zt1o1bcz	360	2	and	and	CC
cord-009318-zt1o1bcz	360	3	N	n	CD
cord-009318-zt1o1bcz	360	4	False	False	NNP
cord-009318-zt1o1bcz	360	5	are	be	VBP
cord-009318-zt1o1bcz	360	6	determined	determine	VBN
cord-009318-zt1o1bcz	360	7	empirically	empirically	RB
cord-009318-zt1o1bcz	360	8	,	,	,
cord-009318-zt1o1bcz	360	9	whereas	whereas	IN
cord-009318-zt1o1bcz	360	10	N	N	NNP
cord-009318-zt1o1bcz	360	11	CI	CI	NNP
cord-009318-zt1o1bcz	360	12	is	be	VBZ
cord-009318-zt1o1bcz	360	13	calculated	calculate	VBN
cord-009318-zt1o1bcz	360	14	from	from	IN
cord-009318-zt1o1bcz	360	15	the	the	DT
cord-009318-zt1o1bcz	360	16	desired	desire	VBN
cord-009318-zt1o1bcz	360	17	expected	expected	JJ
cord-009318-zt1o1bcz	360	18	number	number	NN
cord-009318-zt1o1bcz	360	19	of	of	IN
cord-009318-zt1o1bcz	360	20	molecules	molecule	NNS
cord-009318-zt1o1bcz	360	21	that	that	WDT
cord-009318-zt1o1bcz	360	22	will	will	MD
cord-009318-zt1o1bcz	360	23	yield	yield	VB
cord-009318-zt1o1bcz	360	24	at	at	RB
cord-009318-zt1o1bcz	360	25	least	least	RBS
cord-009318-zt1o1bcz	360	26	one	one	CD
cord-009318-zt1o1bcz	360	27	detection	detection	NN
cord-009318-zt1o1bcz	360	28	event	event	NN
cord-009318-zt1o1bcz	360	29	for	for	IN
cord-009318-zt1o1bcz	360	30	a	a	DT
cord-009318-zt1o1bcz	360	31	given	give	VBN
cord-009318-zt1o1bcz	360	32	confidence	confidence	NN
cord-009318-zt1o1bcz	360	33	interval	interval	NN
cord-009318-zt1o1bcz	360	34	(	(	-LRB-
cord-009318-zt1o1bcz	360	35	N	N	NNP
cord-009318-zt1o1bcz	360	36	CI	CI	NNP
cord-009318-zt1o1bcz	360	37	)	)	-RRB-
cord-009318-zt1o1bcz	360	38	from	from	IN
cord-009318-zt1o1bcz	360	39	the	the	DT
cord-009318-zt1o1bcz	360	40	Poisson	Poisson	NNP
cord-009318-zt1o1bcz	360	41	equation	equation	NN
cord-009318-zt1o1bcz	360	42	.	.	.
cord-009318-zt1o1bcz	361	1	If	if	IN
cord-009318-zt1o1bcz	361	2	we	-PRON-	PRP
cord-009318-zt1o1bcz	361	3	require	require	VBP
cord-009318-zt1o1bcz	361	4	a	a	DT
cord-009318-zt1o1bcz	361	5	95	95	CD
cord-009318-zt1o1bcz	361	6	%	%	NN
cord-009318-zt1o1bcz	361	7	CI	ci	NN
cord-009318-zt1o1bcz	361	8	to	to	TO
cord-009318-zt1o1bcz	361	9	observe	observe	VB
cord-009318-zt1o1bcz	361	10	a	a	DT
cord-009318-zt1o1bcz	361	11	true	true	JJ
cord-009318-zt1o1bcz	361	12	positive	positive	JJ
cord-009318-zt1o1bcz	361	13	across	across	IN
cord-009318-zt1o1bcz	361	14	an	an	DT
cord-009318-zt1o1bcz	361	15	entire	entire	JJ
cord-009318-zt1o1bcz	361	16	chip	chip	NN
cord-009318-zt1o1bcz	361	17	,	,	,
cord-009318-zt1o1bcz	361	18	the	the	DT
cord-009318-zt1o1bcz	361	19	minimum	minimum	JJ
cord-009318-zt1o1bcz	361	20	number	number	NN
cord-009318-zt1o1bcz	361	21	of	of	IN
cord-009318-zt1o1bcz	361	22	counted	count	VBN
cord-009318-zt1o1bcz	361	23	events	event	NNS
cord-009318-zt1o1bcz	361	24	is	be	VBZ
cord-009318-zt1o1bcz	361	25	3	3	CD
cord-009318-zt1o1bcz	361	26	(	(	-LRB-
cord-009318-zt1o1bcz	361	27	i.e.	i.e.	FW
cord-009318-zt1o1bcz	361	28	5	5	CD
cord-009318-zt1o1bcz	361	29	%	%	NN
cord-009318-zt1o1bcz	361	30	of	of	IN
cord-009318-zt1o1bcz	361	31	the	the	DT
cord-009318-zt1o1bcz	361	32	time	time	NN
cord-009318-zt1o1bcz	361	33	,	,	,
cord-009318-zt1o1bcz	361	34	the	the	DT
cord-009318-zt1o1bcz	361	35	Poisson	Poisson	NNP
cord-009318-zt1o1bcz	361	36	expected	expect	VBD
cord-009318-zt1o1bcz	361	37	loading	loading	NN
cord-009318-zt1o1bcz	361	38	of	of	IN
cord-009318-zt1o1bcz	361	39	three	three	CD
cord-009318-zt1o1bcz	361	40	target	target	NN
cord-009318-zt1o1bcz	361	41	molecules	molecule	NNS
cord-009318-zt1o1bcz	361	42	will	will	MD
cord-009318-zt1o1bcz	361	43	still	still	RB
cord-009318-zt1o1bcz	361	44	measure	measure	VB
cord-009318-zt1o1bcz	361	45	zero	zero	CD
cord-009318-zt1o1bcz	361	46	events	event	NNS
cord-009318-zt1o1bcz	361	47	.	.	.
cord-009318-zt1o1bcz	361	48	)	)	-RRB-
cord-009318-zt1o1bcz	362	1	For	for	IN
cord-009318-zt1o1bcz	362	2	a	a	DT
cord-009318-zt1o1bcz	362	3	98	98	CD
cord-009318-zt1o1bcz	362	4	%	%	NN
cord-009318-zt1o1bcz	362	5	CI	CI	NNP
cord-009318-zt1o1bcz	362	6	,	,	,
cord-009318-zt1o1bcz	362	7	N	N	NNP
cord-009318-zt1o1bcz	362	8	CI	CI	NNP
cord-009318-zt1o1bcz	362	9	would	would	MD
cord-009318-zt1o1bcz	362	10	be	be	VB
cord-009318-zt1o1bcz	362	11	four	four	CD
cord-009318-zt1o1bcz	362	12	counts	count	NNS
cord-009318-zt1o1bcz	362	13	.	.	.
cord-009318-zt1o1bcz	363	1	Hence	hence	RB
cord-009318-zt1o1bcz	363	2	,	,	,
cord-009318-zt1o1bcz	363	3	all	all	DT
cord-009318-zt1o1bcz	363	4	true	true	JJ
cord-009318-zt1o1bcz	363	5	-	-	HYPH
cord-009318-zt1o1bcz	363	6	positive	positive	JJ
cord-009318-zt1o1bcz	363	7	counts	count	NNS
cord-009318-zt1o1bcz	363	8	in	in	IN
cord-009318-zt1o1bcz	363	9	excess	excess	NN
cord-009318-zt1o1bcz	363	10	of	of	IN
cord-009318-zt1o1bcz	363	11	N	N	NNP
cord-009318-zt1o1bcz	363	12	CI	CI	NNP
cord-009318-zt1o1bcz	363	13	are	be	VBP
cord-009318-zt1o1bcz	363	14	counts	count	NNS
cord-009318-zt1o1bcz	363	15	observed	observe	VBN
cord-009318-zt1o1bcz	363	16	above	above	IN
cord-009318-zt1o1bcz	363	17	the	the	DT
cord-009318-zt1o1bcz	363	18	LOD	lod	NN
cord-009318-zt1o1bcz	363	19	.	.	.
cord-009318-zt1o1bcz	364	1	Uncertainty	uncertainty	NN
cord-009318-zt1o1bcz	364	2	in	in	IN
cord-009318-zt1o1bcz	364	3	the	the	DT
cord-009318-zt1o1bcz	364	4	LOD	lod	NN
cord-009318-zt1o1bcz	364	5	is	be	VBZ
cord-009318-zt1o1bcz	364	6	given	give	VBN
cord-009318-zt1o1bcz	364	7	by	by	IN
cord-009318-zt1o1bcz	364	8	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	364	9	Equations	Equations	NNPS
cord-009318-zt1o1bcz	364	10	S1	S1	NNP
cord-009318-zt1o1bcz	364	11	-	-	HYPH
cord-009318-zt1o1bcz	364	12	2	2	CD
cord-009318-zt1o1bcz	364	13	.	.	.
cord-009318-zt1o1bcz	365	1	Counting	count	VBG
cord-009318-zt1o1bcz	365	2	only	only	RB
cord-009318-zt1o1bcz	365	3	true	true	JJ
cord-009318-zt1o1bcz	365	4	positives	positive	NNS
cord-009318-zt1o1bcz	365	5	does	do	VBZ
cord-009318-zt1o1bcz	365	6	not	not	RB
cord-009318-zt1o1bcz	365	7	account	account	VB
cord-009318-zt1o1bcz	365	8	for	for	IN
cord-009318-zt1o1bcz	365	9	interference	interference	NN
cord-009318-zt1o1bcz	365	10	from	from	IN
cord-009318-zt1o1bcz	365	11	false	false	JJ
cord-009318-zt1o1bcz	365	12	positives	positive	NNS
cord-009318-zt1o1bcz	365	13	.	.	.
cord-009318-zt1o1bcz	366	1	In	in	IN
cord-009318-zt1o1bcz	366	2	order	order	NN
cord-009318-zt1o1bcz	366	3	to	to	TO
cord-009318-zt1o1bcz	366	4	meet	meet	VB
cord-009318-zt1o1bcz	366	5	our	-PRON-	PRP$
cord-009318-zt1o1bcz	366	6	minimum	minimum	JJ
cord-009318-zt1o1bcz	366	7	counts	count	NNS
cord-009318-zt1o1bcz	366	8	for	for	IN
cord-009318-zt1o1bcz	366	9	detection	detection	NN
cord-009318-zt1o1bcz	366	10	,	,	,
cord-009318-zt1o1bcz	366	11	our	-PRON-	PRP$
cord-009318-zt1o1bcz	366	12	equation	equation	NN
cord-009318-zt1o1bcz	366	13	must	must	MD
cord-009318-zt1o1bcz	366	14	remove	remove	VB
cord-009318-zt1o1bcz	366	15	false	false	JJ
cord-009318-zt1o1bcz	366	16	counts	count	NNS
cord-009318-zt1o1bcz	366	17	(	(	-LRB-
cord-009318-zt1o1bcz	366	18	N	n	CD
cord-009318-zt1o1bcz	366	19	False	False	NNP
cord-009318-zt1o1bcz	366	20	)	)	-RRB-
cord-009318-zt1o1bcz	366	21	.	.	.
cord-009318-zt1o1bcz	367	1	The	the	DT
cord-009318-zt1o1bcz	367	2	generally	generally	RB
cord-009318-zt1o1bcz	367	3	accepted	accept	VBN
cord-009318-zt1o1bcz	367	4	procedure	procedure	NN
cord-009318-zt1o1bcz	367	5	for	for	IN
cord-009318-zt1o1bcz	367	6	LOD	lod	NN
cord-009318-zt1o1bcz	367	7	calculations	calculation	NNS
cord-009318-zt1o1bcz	367	8	with	with	IN
cord-009318-zt1o1bcz	367	9	a	a	DT
cord-009318-zt1o1bcz	367	10	99.7	99.7	CD
cord-009318-zt1o1bcz	367	11	%	%	NN
cord-009318-zt1o1bcz	367	12	CI	CI	NNP
cord-009318-zt1o1bcz	367	13	is	be	VBZ
cord-009318-zt1o1bcz	367	14	to	to	TO
cord-009318-zt1o1bcz	367	15	assign	assign	VB
cord-009318-zt1o1bcz	367	16	N	N	NNP
cord-009318-zt1o1bcz	367	17	True	true	JJ
cord-009318-zt1o1bcz	367	18	only	only	RB
cord-009318-zt1o1bcz	367	19	when	when	WRB
cord-009318-zt1o1bcz	367	20	the	the	DT
cord-009318-zt1o1bcz	367	21	counts	count	NNS
cord-009318-zt1o1bcz	367	22	exceed	exceed	VBP
cord-009318-zt1o1bcz	367	23	the	the	DT
cord-009318-zt1o1bcz	367	24	background	background	NN
cord-009318-zt1o1bcz	367	25	plus	plus	CC
cord-009318-zt1o1bcz	367	26	three	three	CD
cord-009318-zt1o1bcz	367	27	standard	standard	JJ
cord-009318-zt1o1bcz	367	28	deviations	deviation	NNS
cord-009318-zt1o1bcz	367	29	of	of	IN
cord-009318-zt1o1bcz	367	30	the	the	DT
cord-009318-zt1o1bcz	367	31	background	background	NN
cord-009318-zt1o1bcz	367	32	(	(	-LRB-
cord-009318-zt1o1bcz	367	33	N	n	CD
cord-009318-zt1o1bcz	367	34	False	False	NNP
cord-009318-zt1o1bcz	367	35	+	+	SYM
cord-009318-zt1o1bcz	367	36	3	3	CD
cord-009318-zt1o1bcz	367	37	×	×	NN
cord-009318-zt1o1bcz	367	38	√	√	NNP
cord-009318-zt1o1bcz	367	39	N	n	CD
cord-009318-zt1o1bcz	367	40	False	False	NNP
cord-009318-zt1o1bcz	367	41	)	)	-RRB-
cord-009318-zt1o1bcz	367	42	.	.	.
cord-009318-zt1o1bcz	368	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	368	2	approximate	approximate	VBP
cord-009318-zt1o1bcz	368	3	the	the	DT
cord-009318-zt1o1bcz	368	4	variance	variance	NN
cord-009318-zt1o1bcz	368	5	in	in	IN
cord-009318-zt1o1bcz	368	6	the	the	DT
cord-009318-zt1o1bcz	368	7	background	background	NN
cord-009318-zt1o1bcz	368	8	using	use	VBG
cord-009318-zt1o1bcz	368	9	the	the	DT
cord-009318-zt1o1bcz	368	10	counting	counting	NN
cord-009318-zt1o1bcz	368	11	error	error	NN
cord-009318-zt1o1bcz	368	12	as	as	IN
cord-009318-zt1o1bcz	368	13	three	three	CD
cord-009318-zt1o1bcz	368	14	times	time	NNS
cord-009318-zt1o1bcz	368	15	the	the	DT
cord-009318-zt1o1bcz	368	16	square	square	JJ
cord-009318-zt1o1bcz	368	17	root	root	NN
cord-009318-zt1o1bcz	368	18	of	of	IN
cord-009318-zt1o1bcz	368	19	the	the	DT
cord-009318-zt1o1bcz	368	20	number	number	NN
cord-009318-zt1o1bcz	368	21	of	of	IN
cord-009318-zt1o1bcz	368	22	false	false	JJ
cord-009318-zt1o1bcz	368	23	-	-	HYPH
cord-009318-zt1o1bcz	368	24	positive	positive	JJ
cord-009318-zt1o1bcz	368	25	events	event	NNS
cord-009318-zt1o1bcz	368	26	counted	count	VBN
cord-009318-zt1o1bcz	368	27	and	and	CC
cord-009318-zt1o1bcz	368	28	subtract	subtract	VB
cord-009318-zt1o1bcz	368	29	those	those	DT
cord-009318-zt1o1bcz	368	30	counts	count	NNS
cord-009318-zt1o1bcz	368	31	from	from	IN
cord-009318-zt1o1bcz	368	32	the	the	DT
cord-009318-zt1o1bcz	368	33	true	true	JJ
cord-009318-zt1o1bcz	368	34	-	-	HYPH
cord-009318-zt1o1bcz	368	35	positive	positive	JJ
cord-009318-zt1o1bcz	368	36	counts	count	NNS
cord-009318-zt1o1bcz	368	37	to	to	TO
cord-009318-zt1o1bcz	368	38	yield	yield	VB
cord-009318-zt1o1bcz	368	39	the	the	DT
cord-009318-zt1o1bcz	368	40	equation	equation	NN
cord-009318-zt1o1bcz	368	41	.	.	.
cord-009318-zt1o1bcz	369	1	Using	use	VBG
cord-009318-zt1o1bcz	369	2	this	this	DT
cord-009318-zt1o1bcz	369	3	calculation	calculation	NN
cord-009318-zt1o1bcz	369	4	of	of	IN
cord-009318-zt1o1bcz	369	5	LOD	lod	NN
cord-009318-zt1o1bcz	369	6	to	to	TO
cord-009318-zt1o1bcz	369	7	optimize	optimize	VB
cord-009318-zt1o1bcz	369	8	an	an	DT
cord-009318-zt1o1bcz	369	9	assay	assay	NN
cord-009318-zt1o1bcz	369	10	has	have	VBZ
cord-009318-zt1o1bcz	369	11	three	three	CD
cord-009318-zt1o1bcz	369	12	limitations	limitation	NNS
cord-009318-zt1o1bcz	369	13	.	.	.
cord-009318-zt1o1bcz	370	1	First	first	RB
cord-009318-zt1o1bcz	370	2	,	,	,
cord-009318-zt1o1bcz	370	3	Equation	equation	NN
cord-009318-zt1o1bcz	370	4	(	(	-LRB-
cord-009318-zt1o1bcz	370	5	1	1	CD
cord-009318-zt1o1bcz	370	6	)	)	-RRB-
cord-009318-zt1o1bcz	370	7	fails	fail	VBZ
cord-009318-zt1o1bcz	370	8	to	to	TO
cord-009318-zt1o1bcz	370	9	produce	produce	VB
cord-009318-zt1o1bcz	370	10	a	a	DT
cord-009318-zt1o1bcz	370	11	number	number	NN
cord-009318-zt1o1bcz	370	12	with	with	IN
cord-009318-zt1o1bcz	370	13	physical	physical	JJ
cord-009318-zt1o1bcz	370	14	meaning	meaning	NN
cord-009318-zt1o1bcz	370	15	when	when	WRB
cord-009318-zt1o1bcz	370	16	the	the	DT
cord-009318-zt1o1bcz	370	17	number	number	NN
cord-009318-zt1o1bcz	370	18	of	of	IN
cord-009318-zt1o1bcz	370	19	true	true	JJ
cord-009318-zt1o1bcz	370	20	-	-	HYPH
cord-009318-zt1o1bcz	370	21	positive	positive	JJ
cord-009318-zt1o1bcz	370	22	events	event	NNS
cord-009318-zt1o1bcz	370	23	(	(	-LRB-
cord-009318-zt1o1bcz	370	24	N	N	NNP
cord-009318-zt1o1bcz	370	25	True	true	JJ
cord-009318-zt1o1bcz	370	26	)	)	-RRB-
cord-009318-zt1o1bcz	370	27	is	be	VBZ
cord-009318-zt1o1bcz	370	28	less	less	JJR
cord-009318-zt1o1bcz	370	29	than	than	IN
cord-009318-zt1o1bcz	370	30	the	the	DT
cord-009318-zt1o1bcz	370	31	number	number	NN
cord-009318-zt1o1bcz	370	32	of	of	IN
cord-009318-zt1o1bcz	370	33	false	false	JJ
cord-009318-zt1o1bcz	370	34	-	-	HYPH
cord-009318-zt1o1bcz	370	35	positive	positive	JJ
cord-009318-zt1o1bcz	370	36	events	event	NNS
cord-009318-zt1o1bcz	370	37	plus	plus	CC
cord-009318-zt1o1bcz	370	38	three	three	CD
cord-009318-zt1o1bcz	370	39	times	time	NNS
cord-009318-zt1o1bcz	370	40	the	the	DT
cord-009318-zt1o1bcz	370	41	standard	standard	JJ
cord-009318-zt1o1bcz	370	42	deviation	deviation	NN
cord-009318-zt1o1bcz	370	43	in	in	IN
cord-009318-zt1o1bcz	370	44	false	false	JJ
cord-009318-zt1o1bcz	370	45	amplification	amplification	NN
cord-009318-zt1o1bcz	370	46	(	(	-LRB-
cord-009318-zt1o1bcz	370	47	N	n	CD
cord-009318-zt1o1bcz	370	48	False	False	NNP
cord-009318-zt1o1bcz	370	49	+	+	SYM
cord-009318-zt1o1bcz	370	50	3	3	CD
cord-009318-zt1o1bcz	370	51	×	×	NN
cord-009318-zt1o1bcz	370	52	√	√	NNP
cord-009318-zt1o1bcz	370	53	N	n	CD
cord-009318-zt1o1bcz	370	54	False	False	NNP
cord-009318-zt1o1bcz	370	55	)	)	-RRB-
cord-009318-zt1o1bcz	370	56	.	.	.
cord-009318-zt1o1bcz	371	1	In	in	IN
cord-009318-zt1o1bcz	371	2	this	this	DT
cord-009318-zt1o1bcz	371	3	case	case	NN
cord-009318-zt1o1bcz	371	4	,	,	,
cord-009318-zt1o1bcz	371	5	it	-PRON-	PRP
cord-009318-zt1o1bcz	371	6	is	be	VBZ
cord-009318-zt1o1bcz	371	7	not	not	RB
cord-009318-zt1o1bcz	371	8	possible	possible	JJ
cord-009318-zt1o1bcz	371	9	to	to	TO
cord-009318-zt1o1bcz	371	10	conclusively	conclusively	RB
cord-009318-zt1o1bcz	371	11	observe	observe	VB
cord-009318-zt1o1bcz	371	12	a	a	DT
cord-009318-zt1o1bcz	371	13	true	true	JJ
cord-009318-zt1o1bcz	371	14	positive	positive	JJ
cord-009318-zt1o1bcz	371	15	,	,	,
cord-009318-zt1o1bcz	371	16	and	and	CC
cord-009318-zt1o1bcz	371	17	the	the	DT
cord-009318-zt1o1bcz	371	18	LOD	lod	NN
cord-009318-zt1o1bcz	371	19	becomes	become	VBZ
cord-009318-zt1o1bcz	371	20	irrelevant	irrelevant	JJ
cord-009318-zt1o1bcz	371	21	.	.	.
cord-009318-zt1o1bcz	372	1	Second	second	RB
cord-009318-zt1o1bcz	372	2	,	,	,
cord-009318-zt1o1bcz	372	3	Equation	equation	NN
cord-009318-zt1o1bcz	372	4	(	(	-LRB-
cord-009318-zt1o1bcz	372	5	1	1	CD
cord-009318-zt1o1bcz	372	6	)	)	-RRB-
cord-009318-zt1o1bcz	372	7	gives	give	VBZ
cord-009318-zt1o1bcz	372	8	an	an	DT
cord-009318-zt1o1bcz	372	9	absolute	absolute	JJ
cord-009318-zt1o1bcz	372	10	LOD	lod	NN
cord-009318-zt1o1bcz	372	11	.	.	.
cord-009318-zt1o1bcz	373	1	The	the	DT
cord-009318-zt1o1bcz	373	2	numerator	numerator	NN
cord-009318-zt1o1bcz	373	3	(	(	-LRB-
cord-009318-zt1o1bcz	373	4	concentration	concentration	NN
cord-009318-zt1o1bcz	373	5	of	of	IN
cord-009318-zt1o1bcz	373	6	template	template	NN
cord-009318-zt1o1bcz	373	7	molecules	molecule	NNS
cord-009318-zt1o1bcz	373	8	loaded	load	VBN
cord-009318-zt1o1bcz	373	9	on	on	IN
cord-009318-zt1o1bcz	373	10	the	the	DT
cord-009318-zt1o1bcz	373	11	chip	chip	NN
cord-009318-zt1o1bcz	373	12	,	,	,
cord-009318-zt1o1bcz	373	13	as	as	IN
cord-009318-zt1o1bcz	373	14	determined	determine	VBN
cord-009318-zt1o1bcz	373	15	by	by	IN
cord-009318-zt1o1bcz	373	16	PCR	PCR	NNP
cord-009318-zt1o1bcz	373	17	)	)	-RRB-
cord-009318-zt1o1bcz	373	18	is	be	VBZ
cord-009318-zt1o1bcz	373	19	corrected	correct	VBN
cord-009318-zt1o1bcz	373	20	for	for	IN
cord-009318-zt1o1bcz	373	21	the	the	DT
cord-009318-zt1o1bcz	373	22	probability	probability	NN
cord-009318-zt1o1bcz	373	23	of	of	IN
cord-009318-zt1o1bcz	373	24	observing	observe	VBG
cord-009318-zt1o1bcz	373	25	a	a	DT
cord-009318-zt1o1bcz	373	26	molecule	molecule	NN
cord-009318-zt1o1bcz	373	27	amplify	amplify	NN
cord-009318-zt1o1bcz	373	28	(	(	-LRB-
cord-009318-zt1o1bcz	373	29	efficiency	efficiency	NN
cord-009318-zt1o1bcz	373	30	)	)	-RRB-
cord-009318-zt1o1bcz	373	31	by	by	IN
cord-009318-zt1o1bcz	373	32	the	the	DT
cord-009318-zt1o1bcz	373	33	true	true	JJ
cord-009318-zt1o1bcz	373	34	-	-	HYPH
cord-009318-zt1o1bcz	373	35	positive	positive	JJ
cord-009318-zt1o1bcz	373	36	counts	count	NNS
cord-009318-zt1o1bcz	373	37	.	.	.
cord-009318-zt1o1bcz	374	1	N	n	CD
cord-009318-zt1o1bcz	374	2	False	False	NNP
cord-009318-zt1o1bcz	374	3	accounts	account	VBZ
cord-009318-zt1o1bcz	374	4	for	for	IN
cord-009318-zt1o1bcz	374	5	the	the	DT
cord-009318-zt1o1bcz	374	6	non	non	JJ
cord-009318-zt1o1bcz	374	7	-	-	JJ
cord-009318-zt1o1bcz	374	8	specific	specific	JJ
cord-009318-zt1o1bcz	374	9	amplification	amplification	NN
cord-009318-zt1o1bcz	374	10	,	,	,
cord-009318-zt1o1bcz	374	11	and	and	CC
cord-009318-zt1o1bcz	374	12	N	N	NNP
cord-009318-zt1o1bcz	374	13	CI	CI	NNP
cord-009318-zt1o1bcz	374	14	accounts	account	VBZ
cord-009318-zt1o1bcz	374	15	for	for	IN
cord-009318-zt1o1bcz	374	16	the	the	DT
cord-009318-zt1o1bcz	374	17	Poisson	Poisson	NNP
cord-009318-zt1o1bcz	374	18	probability	probability	NN
cord-009318-zt1o1bcz	374	19	associated	associate	VBN
cord-009318-zt1o1bcz	374	20	with	with	IN
cord-009318-zt1o1bcz	374	21	loading	load	VBG
cord-009318-zt1o1bcz	374	22	a	a	DT
cord-009318-zt1o1bcz	374	23	target	target	NN
cord-009318-zt1o1bcz	374	24	molecule	molecule	NN
cord-009318-zt1o1bcz	374	25	.	.	.
cord-009318-zt1o1bcz	375	1	Third	third	RB
cord-009318-zt1o1bcz	375	2	,	,	,
cord-009318-zt1o1bcz	375	3	this	this	DT
cord-009318-zt1o1bcz	375	4	equation	equation	NN
cord-009318-zt1o1bcz	375	5	is	be	VBZ
cord-009318-zt1o1bcz	375	6	specific	specific	JJ
cord-009318-zt1o1bcz	375	7	to	to	IN
cord-009318-zt1o1bcz	375	8	digital	digital	JJ
cord-009318-zt1o1bcz	375	9	assays	assay	NNS
cord-009318-zt1o1bcz	375	10	.	.	.
cord-009318-zt1o1bcz	376	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	376	2	first	first	RB
cord-009318-zt1o1bcz	376	3	sought	seek	VBD
cord-009318-zt1o1bcz	376	4	to	to	TO
cord-009318-zt1o1bcz	376	5	demonstrate	demonstrate	VB
cord-009318-zt1o1bcz	376	6	the	the	DT
cord-009318-zt1o1bcz	376	7	selection	selection	NN
cord-009318-zt1o1bcz	376	8	of	of	IN
cord-009318-zt1o1bcz	376	9	optimal	optimal	JJ
cord-009318-zt1o1bcz	376	10	parameters	parameter	NNS
cord-009318-zt1o1bcz	376	11	for	for	IN
cord-009318-zt1o1bcz	376	12	situations	situation	NNS
cord-009318-zt1o1bcz	376	13	where	where	WRB
cord-009318-zt1o1bcz	376	14	HRM	HRM	NNP
cord-009318-zt1o1bcz	376	15	is	be	VBZ
cord-009318-zt1o1bcz	376	16	not	not	RB
cord-009318-zt1o1bcz	376	17	incorporated	incorporate	VBN
cord-009318-zt1o1bcz	376	18	into	into	IN
cord-009318-zt1o1bcz	376	19	the	the	DT
cord-009318-zt1o1bcz	376	20	final	final	JJ
cord-009318-zt1o1bcz	376	21	assay	assay	NN
cord-009318-zt1o1bcz	376	22	.	.	.
cord-009318-zt1o1bcz	377	1	Using	use	VBG
cord-009318-zt1o1bcz	377	2	this	this	DT
cord-009318-zt1o1bcz	377	3	process	process	NN
cord-009318-zt1o1bcz	377	4	,	,	,
cord-009318-zt1o1bcz	377	5	one	one	PRP
cord-009318-zt1o1bcz	377	6	can	can	MD
cord-009318-zt1o1bcz	377	7	pick	pick	VB
cord-009318-zt1o1bcz	377	8	any	any	DT
cord-009318-zt1o1bcz	377	9	threshold	threshold	NN
cord-009318-zt1o1bcz	377	10	and	and	CC
cord-009318-zt1o1bcz	377	11	use	use	VB
cord-009318-zt1o1bcz	377	12	T	T	NNP
cord-009318-zt1o1bcz	377	13	m	m	NN
cord-009318-zt1o1bcz	377	14	to	to	TO
cord-009318-zt1o1bcz	377	15	determine	determine	VB
cord-009318-zt1o1bcz	377	16	the	the	DT
cord-009318-zt1o1bcz	377	17	optimal	optimal	JJ
cord-009318-zt1o1bcz	377	18	trade	trade	NN
cord-009318-zt1o1bcz	377	19	-	-	HYPH
cord-009318-zt1o1bcz	377	20	off	off	NN
cord-009318-zt1o1bcz	377	21	between	between	IN
cord-009318-zt1o1bcz	377	22	true	true	JJ
cord-009318-zt1o1bcz	377	23	and	and	CC
cord-009318-zt1o1bcz	377	24	false	false	JJ
cord-009318-zt1o1bcz	377	25	positives	positive	NNS
cord-009318-zt1o1bcz	377	26	.	.	.
cord-009318-zt1o1bcz	378	1	All	all	DT
cord-009318-zt1o1bcz	378	2	initial	initial	JJ
cord-009318-zt1o1bcz	378	3	experiments	experiment	NNS
cord-009318-zt1o1bcz	378	4	testing	test	VBG
cord-009318-zt1o1bcz	378	5	the	the	DT
cord-009318-zt1o1bcz	378	6	utility	utility	NN
cord-009318-zt1o1bcz	378	7	of	of	IN
cord-009318-zt1o1bcz	378	8	LOD	LOD	NNP
cord-009318-zt1o1bcz	378	9	,	,	,
cord-009318-zt1o1bcz	378	10	juxtaposed	juxtapose	VBN
cord-009318-zt1o1bcz	378	11	against	against	IN
cord-009318-zt1o1bcz	378	12	receiver	receiver	NN
cord-009318-zt1o1bcz	378	13	operating	operating	NN
cord-009318-zt1o1bcz	378	14	characteristic	characteristic	NN
cord-009318-zt1o1bcz	378	15	(	(	-LRB-
cord-009318-zt1o1bcz	378	16	ROC	ROC	NNP
cord-009318-zt1o1bcz	378	17	)	)	-RRB-
cord-009318-zt1o1bcz	378	18	curves	curve	NNS
cord-009318-zt1o1bcz	378	19	,	,	,
cord-009318-zt1o1bcz	378	20	to	to	TO
cord-009318-zt1o1bcz	378	21	identify	identify	VB
cord-009318-zt1o1bcz	378	22	optimal	optimal	JJ
cord-009318-zt1o1bcz	378	23	parameters	parameter	NNS
cord-009318-zt1o1bcz	378	24	were	be	VBD
cord-009318-zt1o1bcz	378	25	done	do	VBN
cord-009318-zt1o1bcz	378	26	using	use	VBG
cord-009318-zt1o1bcz	378	27	Bst	Bst	NNP
cord-009318-zt1o1bcz	378	28	3.0	3.0	NNP
cord-009318-zt1o1bcz	378	29	.	.	.
cord-009318-zt1o1bcz	379	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	379	2	began	begin	VBD
cord-009318-zt1o1bcz	379	3	by	by	IN
cord-009318-zt1o1bcz	379	4	determining	determine	VBG
cord-009318-zt1o1bcz	379	5	the	the	DT
cord-009318-zt1o1bcz	379	6	optimal	optimal	JJ
cord-009318-zt1o1bcz	379	7	thresholds	threshold	NNS
cord-009318-zt1o1bcz	379	8	for	for	IN
cord-009318-zt1o1bcz	379	9	max	max	NN
cord-009318-zt1o1bcz	379	10	rate	rate	NN
cord-009318-zt1o1bcz	379	11	,	,	,
cord-009318-zt1o1bcz	379	12	Fluorescence	Fluorescence	NNP
cord-009318-zt1o1bcz	379	13	Intensity	intensity	NN
cord-009318-zt1o1bcz	379	14	,	,	,
cord-009318-zt1o1bcz	379	15	and	and	CC
cord-009318-zt1o1bcz	379	16	amplification	amplification	NN
cord-009318-zt1o1bcz	379	17	time	time	NN
cord-009318-zt1o1bcz	379	18	.	.	.
cord-009318-zt1o1bcz	380	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	380	2	demonstrate	demonstrate	VBP
cord-009318-zt1o1bcz	380	3	optimization	optimization	NN
cord-009318-zt1o1bcz	380	4	of	of	IN
cord-009318-zt1o1bcz	380	5	all	all	DT
cord-009318-zt1o1bcz	380	6	three	three	CD
cord-009318-zt1o1bcz	380	7	parameters	parameter	NNS
cord-009318-zt1o1bcz	380	8	,	,	,
cord-009318-zt1o1bcz	380	9	using	use	VBG
cord-009318-zt1o1bcz	380	10	T	T	NNP
cord-009318-zt1o1bcz	380	11	m	m	NNP
cord-009318-zt1o1bcz	380	12	as	as	IN
cord-009318-zt1o1bcz	380	13	the	the	DT
cord-009318-zt1o1bcz	380	14	arbiter	arbiter	NN
cord-009318-zt1o1bcz	380	15	,	,	,
cord-009318-zt1o1bcz	380	16	to	to	TO
cord-009318-zt1o1bcz	380	17	illustrate	illustrate	VB
cord-009318-zt1o1bcz	380	18	the	the	DT
cord-009318-zt1o1bcz	380	19	utility	utility	NN
cord-009318-zt1o1bcz	380	20	of	of	IN
cord-009318-zt1o1bcz	380	21	our	-PRON-	PRP$
cord-009318-zt1o1bcz	380	22	method	method	NN
cord-009318-zt1o1bcz	380	23	.	.	.
cord-009318-zt1o1bcz	381	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	381	2	tested	test	VBD
cord-009318-zt1o1bcz	381	3	the	the	DT
cord-009318-zt1o1bcz	381	4	use	use	NN
cord-009318-zt1o1bcz	381	5	of	of	IN
cord-009318-zt1o1bcz	381	6	ROC	ROC	NNP
cord-009318-zt1o1bcz	381	7	curves	curve	NNS
cord-009318-zt1o1bcz	381	8	(	(	-LRB-
cord-009318-zt1o1bcz	381	9	commonly	commonly	RB
cord-009318-zt1o1bcz	381	10	used	use	VBN
cord-009318-zt1o1bcz	381	11	to	to	TO
cord-009318-zt1o1bcz	381	12	indicate	indicate	VB
cord-009318-zt1o1bcz	381	13	clinical	clinical	JJ
cord-009318-zt1o1bcz	381	14	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	381	15	and	and	CC
cord-009318-zt1o1bcz	381	16	specificity	specificity	NN
cord-009318-zt1o1bcz	381	17	)	)	-RRB-
cord-009318-zt1o1bcz	381	18	to	to	TO
cord-009318-zt1o1bcz	381	19	compare	compare	VB
cord-009318-zt1o1bcz	381	20	the	the	DT
cord-009318-zt1o1bcz	381	21	performance	performance	NN
cord-009318-zt1o1bcz	381	22	in	in	IN
cord-009318-zt1o1bcz	381	23	response	response	NN
cord-009318-zt1o1bcz	381	24	to	to	IN
cord-009318-zt1o1bcz	381	25	a	a	DT
cord-009318-zt1o1bcz	381	26	given	give	VBN
cord-009318-zt1o1bcz	381	27	parameter	parameter	NN
cord-009318-zt1o1bcz	381	28	.	.	.
cord-009318-zt1o1bcz	382	1	ROC	ROC	NNP
cord-009318-zt1o1bcz	382	2	curves	curve	NNS
cord-009318-zt1o1bcz	382	3	provide	provide	VBP
cord-009318-zt1o1bcz	382	4	a	a	DT
cord-009318-zt1o1bcz	382	5	visual	visual	JJ
cord-009318-zt1o1bcz	382	6	representation	representation	NN
cord-009318-zt1o1bcz	382	7	of	of	IN
cord-009318-zt1o1bcz	382	8	the	the	DT
cord-009318-zt1o1bcz	382	9	ability	ability	NN
cord-009318-zt1o1bcz	382	10	to	to	TO
cord-009318-zt1o1bcz	382	11	distinguish	distinguish	VB
cord-009318-zt1o1bcz	382	12	between	between	IN
cord-009318-zt1o1bcz	382	13	a	a	DT
cord-009318-zt1o1bcz	382	14	true	true	JJ
cord-009318-zt1o1bcz	382	15	-	-	HYPH
cord-009318-zt1o1bcz	382	16	positive	positive	JJ
cord-009318-zt1o1bcz	382	17	and	and	CC
cord-009318-zt1o1bcz	382	18	false	false	JJ
cord-009318-zt1o1bcz	382	19	-	-	HYPH
cord-009318-zt1o1bcz	382	20	positive	positive	JJ
cord-009318-zt1o1bcz	382	21	event	event	NN
cord-009318-zt1o1bcz	382	22	,	,	,
cord-009318-zt1o1bcz	382	23	as	as	IN
cord-009318-zt1o1bcz	382	24	a	a	DT
cord-009318-zt1o1bcz	382	25	function	function	NN
cord-009318-zt1o1bcz	382	26	of	of	IN
cord-009318-zt1o1bcz	382	27	a	a	DT
cord-009318-zt1o1bcz	382	28	given	give	VBN
cord-009318-zt1o1bcz	382	29	threshold	threshold	NN
cord-009318-zt1o1bcz	382	30	,	,	,
cord-009318-zt1o1bcz	382	31	but	but	CC
cord-009318-zt1o1bcz	382	32	can	can	MD
cord-009318-zt1o1bcz	382	33	be	be	VB
cord-009318-zt1o1bcz	382	34	difficult	difficult	JJ
cord-009318-zt1o1bcz	382	35	to	to	TO
cord-009318-zt1o1bcz	382	36	use	use	VB
cord-009318-zt1o1bcz	382	37	for	for	IN
cord-009318-zt1o1bcz	382	38	optimal	optimal	JJ
cord-009318-zt1o1bcz	382	39	selection	selection	NN
cord-009318-zt1o1bcz	382	40	of	of	IN
cord-009318-zt1o1bcz	382	41	LOD	lod	NN
cord-009318-zt1o1bcz	382	42	.	.	.
cord-009318-zt1o1bcz	383	1	ROC	ROC	NNP
cord-009318-zt1o1bcz	383	2	curves	curve	NNS
cord-009318-zt1o1bcz	383	3	show	show	VBP
cord-009318-zt1o1bcz	383	4	the	the	DT
cord-009318-zt1o1bcz	383	5	fractions	fraction	NNS
cord-009318-zt1o1bcz	383	6	of	of	IN
cord-009318-zt1o1bcz	383	7	true	true	JJ
cord-009318-zt1o1bcz	383	8	and	and	CC
cord-009318-zt1o1bcz	383	9	false	false	JJ
cord-009318-zt1o1bcz	383	10	positives	positive	NNS
cord-009318-zt1o1bcz	383	11	,	,	,
cord-009318-zt1o1bcz	383	12	where	where	WRB
cord-009318-zt1o1bcz	383	13	the	the	DT
cord-009318-zt1o1bcz	383	14	true	true	JJ
cord-009318-zt1o1bcz	383	15	-	-	HYPH
cord-009318-zt1o1bcz	383	16	positive	positive	JJ
cord-009318-zt1o1bcz	383	17	fraction	fraction	NN
cord-009318-zt1o1bcz	383	18	is	be	VBZ
cord-009318-zt1o1bcz	383	19	the	the	DT
cord-009318-zt1o1bcz	383	20	number	number	NN
cord-009318-zt1o1bcz	383	21	of	of	IN
cord-009318-zt1o1bcz	383	22	true	true	JJ
cord-009318-zt1o1bcz	383	23	positives	positive	NNS
cord-009318-zt1o1bcz	383	24	at	at	IN
cord-009318-zt1o1bcz	383	25	a	a	DT
cord-009318-zt1o1bcz	383	26	given	give	VBN
cord-009318-zt1o1bcz	383	27	threshold	threshold	NN
cord-009318-zt1o1bcz	383	28	out	out	IN
cord-009318-zt1o1bcz	383	29	of	of	IN
cord-009318-zt1o1bcz	383	30	the	the	DT
cord-009318-zt1o1bcz	383	31	total	total	JJ
cord-009318-zt1o1bcz	383	32	number	number	NN
cord-009318-zt1o1bcz	383	33	of	of	IN
cord-009318-zt1o1bcz	383	34	true	true	JJ
cord-009318-zt1o1bcz	383	35	positives	positive	NNS
cord-009318-zt1o1bcz	383	36	observed	observe	VBN
cord-009318-zt1o1bcz	383	37	by	by	IN
cord-009318-zt1o1bcz	383	38	T	T	NNP
cord-009318-zt1o1bcz	383	39	m	m	NNP
cord-009318-zt1o1bcz	383	40	;	;	:
cord-009318-zt1o1bcz	383	41	and	and	CC
cord-009318-zt1o1bcz	383	42	the	the	DT
cord-009318-zt1o1bcz	383	43	falsepositive	falsepositive	JJ
cord-009318-zt1o1bcz	383	44	fraction	fraction	NN
cord-009318-zt1o1bcz	383	45	is	be	VBZ
cord-009318-zt1o1bcz	383	46	the	the	DT
cord-009318-zt1o1bcz	383	47	number	number	NN
cord-009318-zt1o1bcz	383	48	of	of	IN
cord-009318-zt1o1bcz	383	49	false	false	JJ
cord-009318-zt1o1bcz	383	50	positives	positive	NNS
cord-009318-zt1o1bcz	383	51	counted	count	VBN
cord-009318-zt1o1bcz	383	52	at	at	IN
cord-009318-zt1o1bcz	383	53	the	the	DT
cord-009318-zt1o1bcz	383	54	given	give	VBN
cord-009318-zt1o1bcz	383	55	threshold	threshold	NN
cord-009318-zt1o1bcz	383	56	,	,	,
cord-009318-zt1o1bcz	383	57	divided	divide	VBN
cord-009318-zt1o1bcz	383	58	by	by	IN
cord-009318-zt1o1bcz	383	59	the	the	DT
cord-009318-zt1o1bcz	383	60	total	total	JJ
cord-009318-zt1o1bcz	383	61	number	number	NN
cord-009318-zt1o1bcz	383	62	of	of	IN
cord-009318-zt1o1bcz	383	63	false	false	JJ
cord-009318-zt1o1bcz	383	64	positives	positive	NNS
cord-009318-zt1o1bcz	383	65	observed	observe	VBN
cord-009318-zt1o1bcz	383	66	by	by	IN
cord-009318-zt1o1bcz	383	67	T	T	NNP
cord-009318-zt1o1bcz	383	68	m	m	NNP
cord-009318-zt1o1bcz	383	69	.	.	.
cord-009318-zt1o1bcz	384	1	A	a	DT
cord-009318-zt1o1bcz	384	2	perfect	perfect	JJ
cord-009318-zt1o1bcz	384	3	classifying	classifying	NN
cord-009318-zt1o1bcz	384	4	test	test	NN
cord-009318-zt1o1bcz	384	5	will	will	MD
cord-009318-zt1o1bcz	384	6	yield	yield	VB
cord-009318-zt1o1bcz	384	7	the	the	DT
cord-009318-zt1o1bcz	384	8	largest	large	JJS
cord-009318-zt1o1bcz	384	9	true	true	JJ
cord-009318-zt1o1bcz	384	10	-	-	HYPH
cord-009318-zt1o1bcz	384	11	positive	positive	JJ
cord-009318-zt1o1bcz	384	12	fraction	fraction	NN
cord-009318-zt1o1bcz	384	13	and	and	CC
cord-009318-zt1o1bcz	384	14	smallest	small	JJS
cord-009318-zt1o1bcz	384	15	false	false	JJ
cord-009318-zt1o1bcz	384	16	-	-	HYPH
cord-009318-zt1o1bcz	384	17	positive	positive	JJ
cord-009318-zt1o1bcz	384	18	fraction	fraction	NN
cord-009318-zt1o1bcz	384	19	.	.	.
cord-009318-zt1o1bcz	385	1	When	when	WRB
cord-009318-zt1o1bcz	385	2	plotting	plot	VBG
cord-009318-zt1o1bcz	385	3	the	the	DT
cord-009318-zt1o1bcz	385	4	ROC	ROC	NNP
cord-009318-zt1o1bcz	385	5	curve	curve	NN
cord-009318-zt1o1bcz	385	6	for	for	IN
cord-009318-zt1o1bcz	385	7	maximum	maximum	JJ
cord-009318-zt1o1bcz	385	8	rate	rate	NN
cord-009318-zt1o1bcz	385	9	(	(	-LRB-
cord-009318-zt1o1bcz	385	10	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	385	11	Figure	Figure	NNP
cord-009318-zt1o1bcz	385	12	S9A	s9a	CD
cord-009318-zt1o1bcz	385	13	)	)	-RRB-
cord-009318-zt1o1bcz	385	14	,	,	,
cord-009318-zt1o1bcz	385	15	we	-PRON-	PRP
cord-009318-zt1o1bcz	385	16	observed	observe	VBD
cord-009318-zt1o1bcz	385	17	that	that	DT
cord-009318-zt1o1bcz	385	18	rate	rate	NN
cord-009318-zt1o1bcz	385	19	initially	initially	RB
cord-009318-zt1o1bcz	385	20	performs	perform	VBZ
cord-009318-zt1o1bcz	385	21	very	very	RB
cord-009318-zt1o1bcz	385	22	well	well	RB
cord-009318-zt1o1bcz	385	23	for	for	IN
cord-009318-zt1o1bcz	385	24	eliminating	eliminate	VBG
cord-009318-zt1o1bcz	385	25	false	false	JJ
cord-009318-zt1o1bcz	385	26	positives	positive	NNS
cord-009318-zt1o1bcz	385	27	(	(	-LRB-
cord-009318-zt1o1bcz	385	28	the	the	DT
cord-009318-zt1o1bcz	385	29	falsepositive	falsepositive	JJ
cord-009318-zt1o1bcz	385	30	fraction	fraction	NN
cord-009318-zt1o1bcz	385	31	is	be	VBZ
cord-009318-zt1o1bcz	385	32	very	very	RB
cord-009318-zt1o1bcz	385	33	small	small	JJ
cord-009318-zt1o1bcz	385	34	for	for	IN
cord-009318-zt1o1bcz	385	35	very	very	RB
cord-009318-zt1o1bcz	385	36	high	high	JJ
cord-009318-zt1o1bcz	385	37	rates	rate	NNS
cord-009318-zt1o1bcz	385	38	)	)	-RRB-
cord-009318-zt1o1bcz	385	39	.	.	.
cord-009318-zt1o1bcz	386	1	However	however	RB
cord-009318-zt1o1bcz	386	2	,	,	,
cord-009318-zt1o1bcz	386	3	as	as	IN
cord-009318-zt1o1bcz	386	4	the	the	DT
cord-009318-zt1o1bcz	386	5	digital	digital	JJ
cord-009318-zt1o1bcz	386	6	threshold	threshold	NN
cord-009318-zt1o1bcz	386	7	(	(	-LRB-
cord-009318-zt1o1bcz	386	8	analogous	analogous	JJ
cord-009318-zt1o1bcz	386	9	to	to	IN
cord-009318-zt1o1bcz	386	10	ROC	ROC	NNP
cord-009318-zt1o1bcz	386	11	'	'	POS
cord-009318-zt1o1bcz	386	12	cut	cut	NN
cord-009318-zt1o1bcz	386	13	-	-	HYPH
cord-009318-zt1o1bcz	386	14	point	point	NN
cord-009318-zt1o1bcz	386	15	'	'	''
cord-009318-zt1o1bcz	386	16	)	)	-RRB-
cord-009318-zt1o1bcz	386	17	for	for	IN
cord-009318-zt1o1bcz	386	18	rate	rate	NN
cord-009318-zt1o1bcz	386	19	decreases	decrease	NNS
cord-009318-zt1o1bcz	386	20	,	,	,
cord-009318-zt1o1bcz	386	21	a	a	DT
cord-009318-zt1o1bcz	386	22	greater	great	JJR
cord-009318-zt1o1bcz	386	23	number	number	NN
cord-009318-zt1o1bcz	386	24	of	of	IN
cord-009318-zt1o1bcz	386	25	both	both	DT
cord-009318-zt1o1bcz	386	26	false	false	JJ
cord-009318-zt1o1bcz	386	27	and	and	CC
cord-009318-zt1o1bcz	386	28	truepositive	truepositive	JJ
cord-009318-zt1o1bcz	386	29	values	value	NNS
cord-009318-zt1o1bcz	386	30	are	be	VBP
cord-009318-zt1o1bcz	386	31	counted	count	VBN
cord-009318-zt1o1bcz	386	32	.	.	.
cord-009318-zt1o1bcz	387	1	Closer	close	JJR
cord-009318-zt1o1bcz	387	2	examination	examination	NN
cord-009318-zt1o1bcz	387	3	of	of	IN
cord-009318-zt1o1bcz	387	4	this	this	DT
cord-009318-zt1o1bcz	387	5	range	range	NN
cord-009318-zt1o1bcz	387	6	of	of	IN
cord-009318-zt1o1bcz	387	7	thresholds	threshold	NNS
cord-009318-zt1o1bcz	387	8	(	(	-LRB-
cord-009318-zt1o1bcz	387	9	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	387	10	Figure	Figure	NNP
cord-009318-zt1o1bcz	387	11	S9B	s9b	CD
cord-009318-zt1o1bcz	387	12	)	)	-RRB-
cord-009318-zt1o1bcz	387	13	emphasizes	emphasize	VBZ
cord-009318-zt1o1bcz	387	14	the	the	DT
cord-009318-zt1o1bcz	387	15	Youden	Youden	NNP
cord-009318-zt1o1bcz	387	16	Index	Index	NNP
cord-009318-zt1o1bcz	387	17	at	at	IN
cord-009318-zt1o1bcz	387	18	34.6	34.6	CD
cord-009318-zt1o1bcz	387	19	true	true	JJ
cord-009318-zt1o1bcz	387	20	-	-	HYPH
cord-009318-zt1o1bcz	387	21	positive	positive	JJ
cord-009318-zt1o1bcz	387	22	fraction	fraction	NN
cord-009318-zt1o1bcz	387	23	and	and	CC
cord-009318-zt1o1bcz	387	24	4.6	4.6	CD
cord-009318-zt1o1bcz	387	25	false	false	JJ
cord-009318-zt1o1bcz	387	26	-	-	HYPH
cord-009318-zt1o1bcz	387	27	positive	positive	JJ
cord-009318-zt1o1bcz	387	28	fraction	fraction	NN
cord-009318-zt1o1bcz	387	29	as	as	IN
cord-009318-zt1o1bcz	387	30	a	a	DT
cord-009318-zt1o1bcz	387	31	possible	possible	JJ
cord-009318-zt1o1bcz	387	32	choice	choice	NN
cord-009318-zt1o1bcz	387	33	for	for	IN
cord-009318-zt1o1bcz	387	34	optimum	optimum	JJ
cord-009318-zt1o1bcz	387	35	threshold	threshold	NN
cord-009318-zt1o1bcz	387	36	,	,	,
cord-009318-zt1o1bcz	387	37	although	although	IN
cord-009318-zt1o1bcz	387	38	the	the	DT
cord-009318-zt1o1bcz	387	39	assay	assay	NN
cord-009318-zt1o1bcz	387	40	performance	performance	NN
cord-009318-zt1o1bcz	387	41	in	in	IN
cord-009318-zt1o1bcz	387	42	terms	term	NNS
cord-009318-zt1o1bcz	387	43	of	of	IN
cord-009318-zt1o1bcz	387	44	LOD	LOD	NNP
cord-009318-zt1o1bcz	387	45	is	be	VBZ
cord-009318-zt1o1bcz	387	46	unclear	unclear	JJ
cord-009318-zt1o1bcz	387	47	.	.	.
cord-009318-zt1o1bcz	388	1	The	the	DT
cord-009318-zt1o1bcz	388	2	choice	choice	NN
cord-009318-zt1o1bcz	388	3	for	for	IN
cord-009318-zt1o1bcz	388	4	optimal	optimal	JJ
cord-009318-zt1o1bcz	388	5	final	final	JJ
cord-009318-zt1o1bcz	388	6	-	-	HYPH
cord-009318-zt1o1bcz	388	7	intensity	intensity	NN
cord-009318-zt1o1bcz	388	8	threshold	threshold	NN
cord-009318-zt1o1bcz	388	9	is	be	VBZ
cord-009318-zt1o1bcz	388	10	even	even	RB
cord-009318-zt1o1bcz	388	11	less	less	RBR
cord-009318-zt1o1bcz	388	12	clear	clear	JJ
cord-009318-zt1o1bcz	388	13	with	with	IN
cord-009318-zt1o1bcz	388	14	the	the	DT
cord-009318-zt1o1bcz	388	15	ROC	ROC	NNP
cord-009318-zt1o1bcz	388	16	curve	curve	NN
cord-009318-zt1o1bcz	388	17	(	(	-LRB-
cord-009318-zt1o1bcz	388	18	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	388	19	Figure	Figure	NNP
cord-009318-zt1o1bcz	388	20	S9C	S9C	NNS
cord-009318-zt1o1bcz	388	21	)	)	-RRB-
cord-009318-zt1o1bcz	388	22	,	,	,
cord-009318-zt1o1bcz	388	23	as	as	IN
cord-009318-zt1o1bcz	388	24	the	the	DT
cord-009318-zt1o1bcz	388	25	ROC	ROC	NNP
cord-009318-zt1o1bcz	388	26	curves	curve	NNS
cord-009318-zt1o1bcz	388	27	do	do	VBP
cord-009318-zt1o1bcz	388	28	not	not	RB
cord-009318-zt1o1bcz	388	29	give	give	VB
cord-009318-zt1o1bcz	388	30	clear	clear	JJ
cord-009318-zt1o1bcz	388	31	indication	indication	NN
cord-009318-zt1o1bcz	388	32	of	of	IN
cord-009318-zt1o1bcz	388	33	the	the	DT
cord-009318-zt1o1bcz	388	34	optimal	optimal	JJ
cord-009318-zt1o1bcz	388	35	LOD	lod	NN
cord-009318-zt1o1bcz	388	36	(	(	-LRB-
cord-009318-zt1o1bcz	388	37	the	the	DT
cord-009318-zt1o1bcz	388	38	ROC	ROC	NNP
cord-009318-zt1o1bcz	388	39	curve	curve	NN
cord-009318-zt1o1bcz	388	40	is	be	VBZ
cord-009318-zt1o1bcz	388	41	a	a	DT
cord-009318-zt1o1bcz	388	42	gentle	gentle	JJ
cord-009318-zt1o1bcz	388	43	concave	concave	JJ
cord-009318-zt1o1bcz	388	44	slope	slope	NN
cord-009318-zt1o1bcz	388	45	)	)	-RRB-
cord-009318-zt1o1bcz	388	46	.	.	.
cord-009318-zt1o1bcz	389	1	Even	even	RB
cord-009318-zt1o1bcz	389	2	relatively	relatively	RB
cord-009318-zt1o1bcz	389	3	high	high	JJ
cord-009318-zt1o1bcz	389	4	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	389	5	thresholds	threshold	NNS
cord-009318-zt1o1bcz	389	6	do	do	VBP
cord-009318-zt1o1bcz	389	7	not	not	RB
cord-009318-zt1o1bcz	389	8	give	give	VB
cord-009318-zt1o1bcz	389	9	indications	indication	NNS
cord-009318-zt1o1bcz	389	10	of	of	IN
cord-009318-zt1o1bcz	389	11	the	the	DT
cord-009318-zt1o1bcz	389	12	optimal	optimal	JJ
cord-009318-zt1o1bcz	389	13	cut	cut	NN
cord-009318-zt1o1bcz	389	14	-	-	HYPH
cord-009318-zt1o1bcz	389	15	point	point	NN
cord-009318-zt1o1bcz	389	16	(	(	-LRB-
cord-009318-zt1o1bcz	389	17	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	389	18	Figure	figure	NN
cord-009318-zt1o1bcz	389	19	 	 	_SP
cord-009318-zt1o1bcz	389	20	S9D	s9d	NN
cord-009318-zt1o1bcz	389	21	)	)	-RRB-
cord-009318-zt1o1bcz	389	22	.	.	.
cord-009318-zt1o1bcz	390	1	Filtering	filter	VBG
cord-009318-zt1o1bcz	390	2	using	use	VBG
cord-009318-zt1o1bcz	390	3	LOD	LOD	NNP
cord-009318-zt1o1bcz	390	4	revealed	reveal	VBD
cord-009318-zt1o1bcz	390	5	a	a	DT
cord-009318-zt1o1bcz	390	6	clear	clear	JJ
cord-009318-zt1o1bcz	390	7	optimum	optimum	NN
cord-009318-zt1o1bcz	390	8	.	.	.
cord-009318-zt1o1bcz	391	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	391	2	plot	plot	VBP
cord-009318-zt1o1bcz	391	3	the	the	DT
cord-009318-zt1o1bcz	391	4	total	total	JJ
cord-009318-zt1o1bcz	391	5	number	number	NN
cord-009318-zt1o1bcz	391	6	of	of	IN
cord-009318-zt1o1bcz	391	7	events	event	NNS
cord-009318-zt1o1bcz	391	8	for	for	IN
cord-009318-zt1o1bcz	391	9	both	both	CC
cord-009318-zt1o1bcz	391	10	true	true	JJ
cord-009318-zt1o1bcz	391	11	and	and	CC
cord-009318-zt1o1bcz	391	12	false	false	JJ
cord-009318-zt1o1bcz	391	13	positives	positive	NNS
cord-009318-zt1o1bcz	391	14	and	and	CC
cord-009318-zt1o1bcz	391	15	LOD	lod	NN
cord-009318-zt1o1bcz	391	16	as	as	IN
cord-009318-zt1o1bcz	391	17	a	a	DT
cord-009318-zt1o1bcz	391	18	function	function	NN
cord-009318-zt1o1bcz	391	19	of	of	IN
cord-009318-zt1o1bcz	391	20	maximum	maximum	JJ
cord-009318-zt1o1bcz	391	21	rate	rate	NN
cord-009318-zt1o1bcz	391	22	(	(	-LRB-
cord-009318-zt1o1bcz	391	23	Figure	figure	NN
cord-009318-zt1o1bcz	391	24	7A	7a	NN
cord-009318-zt1o1bcz	391	25	)	)	-RRB-
cord-009318-zt1o1bcz	391	26	.	.	.
cord-009318-zt1o1bcz	392	1	The	the	DT
cord-009318-zt1o1bcz	392	2	LOD	LOD	NNP
cord-009318-zt1o1bcz	392	3	curve	curve	NN
cord-009318-zt1o1bcz	392	4	revealed	reveal	VBD
cord-009318-zt1o1bcz	392	5	a	a	DT
cord-009318-zt1o1bcz	392	6	clear	clear	JJ
cord-009318-zt1o1bcz	392	7	minima	minima	NN
cord-009318-zt1o1bcz	392	8	,	,	,
cord-009318-zt1o1bcz	392	9	corresponding	correspond	VBG
cord-009318-zt1o1bcz	392	10	to	to	IN
cord-009318-zt1o1bcz	392	11	the	the	DT
cord-009318-zt1o1bcz	392	12	optimal	optimal	JJ
cord-009318-zt1o1bcz	392	13	cut	cut	NN
cord-009318-zt1o1bcz	392	14	-	-	HYPH
cord-009318-zt1o1bcz	392	15	point	point	NN
cord-009318-zt1o1bcz	392	16	using	use	VBG
cord-009318-zt1o1bcz	392	17	rate	rate	NN
cord-009318-zt1o1bcz	392	18	.	.	.
cord-009318-zt1o1bcz	393	1	Selecting	select	VBG
cord-009318-zt1o1bcz	393	2	the	the	DT
cord-009318-zt1o1bcz	393	3	threshold	threshold	NN
cord-009318-zt1o1bcz	393	4	of	of	IN
cord-009318-zt1o1bcz	393	5	49.8	49.8	CD
cord-009318-zt1o1bcz	393	6	RFU/30	RFU/30	NNS
cord-009318-zt1o1bcz	393	7	s	s	NNP
cord-009318-zt1o1bcz	393	8	generated	generate	VBD
cord-009318-zt1o1bcz	393	9	an	an	DT
cord-009318-zt1o1bcz	393	10	LOD	lod	NN
cord-009318-zt1o1bcz	393	11	of	of	IN
cord-009318-zt1o1bcz	393	12	2.11	2.11	CD
cord-009318-zt1o1bcz	393	13	±	±	CD
cord-009318-zt1o1bcz	393	14	0.92	0.92	CD
cord-009318-zt1o1bcz	394	1	cp	cp	NNP
cord-009318-zt1o1bcz	394	2	/	/	SYM
cord-009318-zt1o1bcz	394	3	l	l	NNP
cord-009318-zt1o1bcz	394	4	.	.	.
cord-009318-zt1o1bcz	395	1	Similarly	similarly	RB
cord-009318-zt1o1bcz	395	2	,	,	,
cord-009318-zt1o1bcz	395	3	plotting	plot	VBG
cord-009318-zt1o1bcz	395	4	LOD	lod	NN
cord-009318-zt1o1bcz	395	5	against	against	IN
cord-009318-zt1o1bcz	395	6	final	final	JJ
cord-009318-zt1o1bcz	395	7	intensity	intensity	NN
cord-009318-zt1o1bcz	395	8	resulted	result	VBD
cord-009318-zt1o1bcz	395	9	in	in	IN
cord-009318-zt1o1bcz	395	10	a	a	DT
cord-009318-zt1o1bcz	395	11	clear	clear	JJ
cord-009318-zt1o1bcz	395	12	minima	minima	NN
cord-009318-zt1o1bcz	395	13	,	,	,
cord-009318-zt1o1bcz	395	14	despite	despite	IN
cord-009318-zt1o1bcz	395	15	the	the	DT
cord-009318-zt1o1bcz	395	16	histogram	histogram	NN
cord-009318-zt1o1bcz	395	17	appearing	appear	VBG
cord-009318-zt1o1bcz	395	18	as	as	IN
cord-009318-zt1o1bcz	395	19	a	a	DT
cord-009318-zt1o1bcz	395	20	continuum	continuum	NN
cord-009318-zt1o1bcz	395	21	and	and	CC
cord-009318-zt1o1bcz	395	22	the	the	DT
cord-009318-zt1o1bcz	395	23	cut	cut	NN
cord-009318-zt1o1bcz	395	24	-	-	HYPH
cord-009318-zt1o1bcz	395	25	point	point	NN
cord-009318-zt1o1bcz	395	26	being	being	NN
cord-009318-zt1o1bcz	395	27	thus	thus	RB
cord-009318-zt1o1bcz	395	28	ambiguous	ambiguous	JJ
cord-009318-zt1o1bcz	395	29	(	(	-LRB-
cord-009318-zt1o1bcz	395	30	Figure	figure	NN
cord-009318-zt1o1bcz	395	31	7B	7b	NN
cord-009318-zt1o1bcz	395	32	)	)	-RRB-
cord-009318-zt1o1bcz	395	33	.	.	.
cord-009318-zt1o1bcz	396	1	Using	use	VBG
cord-009318-zt1o1bcz	396	2	final	final	JJ
cord-009318-zt1o1bcz	396	3	intensity	intensity	NN
cord-009318-zt1o1bcz	396	4	,	,	,
cord-009318-zt1o1bcz	396	5	an	an	DT
cord-009318-zt1o1bcz	396	6	LOD	lod	NN
cord-009318-zt1o1bcz	396	7	of	of	IN
cord-009318-zt1o1bcz	396	8	2.14	2.14	CD
cord-009318-zt1o1bcz	396	9	±	±	CD
cord-009318-zt1o1bcz	396	10	0.89	0.89	CD
cord-009318-zt1o1bcz	396	11	cp	cp	NNP
cord-009318-zt1o1bcz	396	12	/	/	CC
cord-009318-zt1o1bcz	396	13	l	l	NN
cord-009318-zt1o1bcz	396	14	can	can	MD
cord-009318-zt1o1bcz	396	15	be	be	VB
cord-009318-zt1o1bcz	396	16	achieved	achieve	VBN
cord-009318-zt1o1bcz	396	17	at	at	IN
cord-009318-zt1o1bcz	396	18	1393	1393	CD
cord-009318-zt1o1bcz	396	19	RFU	rfu	NN
cord-009318-zt1o1bcz	396	20	.	.	.
cord-009318-zt1o1bcz	397	1	The	the	DT
cord-009318-zt1o1bcz	397	2	ROC	ROC	NNP
cord-009318-zt1o1bcz	397	3	curve	curve	NN
cord-009318-zt1o1bcz	397	4	for	for	IN
cord-009318-zt1o1bcz	397	5	TTP	TTP	NNP
cord-009318-zt1o1bcz	397	6	presented	present	VBD
cord-009318-zt1o1bcz	397	7	a	a	DT
cord-009318-zt1o1bcz	397	8	narrow	narrow	JJ
cord-009318-zt1o1bcz	397	9	range	range	NN
cord-009318-zt1o1bcz	397	10	of	of	IN
cord-009318-zt1o1bcz	397	11	thresholds	threshold	NNS
cord-009318-zt1o1bcz	397	12	,	,	,
cord-009318-zt1o1bcz	397	13	with	with	IN
cord-009318-zt1o1bcz	397	14	∼50	∼50	NN
cord-009318-zt1o1bcz	397	15	%	%	NN
cord-009318-zt1o1bcz	397	16	true	true	JJ
cord-009318-zt1o1bcz	397	17	-	-	HYPH
cord-009318-zt1o1bcz	397	18	positive	positive	JJ
cord-009318-zt1o1bcz	397	19	fraction	fraction	NN
cord-009318-zt1o1bcz	397	20	and	and	CC
cord-009318-zt1o1bcz	397	21	2	2	CD
cord-009318-zt1o1bcz	397	22	%	%	NN
cord-009318-zt1o1bcz	397	23	falsepositive	falsepositive	JJ
cord-009318-zt1o1bcz	397	24	fraction	fraction	NN
cord-009318-zt1o1bcz	397	25	,	,	,
cord-009318-zt1o1bcz	397	26	although	although	IN
cord-009318-zt1o1bcz	397	27	the	the	DT
cord-009318-zt1o1bcz	397	28	precise	precise	JJ
cord-009318-zt1o1bcz	397	29	optimal	optimal	JJ
cord-009318-zt1o1bcz	397	30	threshold	threshold	NN
cord-009318-zt1o1bcz	397	31	was	be	VBD
cord-009318-zt1o1bcz	397	32	not	not	RB
cord-009318-zt1o1bcz	397	33	obvious	obvious	JJ
cord-009318-zt1o1bcz	397	34	(	(	-LRB-
cord-009318-zt1o1bcz	397	35	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	397	36	Figure	Figure	NNP
cord-009318-zt1o1bcz	397	37	S9E	S9E	NNP
cord-009318-zt1o1bcz	397	38	)	)	-RRB-
cord-009318-zt1o1bcz	397	39	.	.	.
cord-009318-zt1o1bcz	398	1	To	to	TO
cord-009318-zt1o1bcz	398	2	refine	refine	VB
cord-009318-zt1o1bcz	398	3	this	this	DT
cord-009318-zt1o1bcz	398	4	threshold	threshold	NN
cord-009318-zt1o1bcz	398	5	,	,	,
cord-009318-zt1o1bcz	398	6	we	-PRON-	PRP
cord-009318-zt1o1bcz	398	7	plotted	plot	VBD
cord-009318-zt1o1bcz	398	8	the	the	DT
cord-009318-zt1o1bcz	398	9	LOD	lod	NN
cord-009318-zt1o1bcz	398	10	and	and	CC
cord-009318-zt1o1bcz	398	11	the	the	DT
cord-009318-zt1o1bcz	398	12	cumulative	cumulative	JJ
cord-009318-zt1o1bcz	398	13	counts	count	NNS
cord-009318-zt1o1bcz	398	14	as	as	IN
cord-009318-zt1o1bcz	398	15	a	a	DT
cord-009318-zt1o1bcz	398	16	function	function	NN
cord-009318-zt1o1bcz	398	17	of	of	IN
cord-009318-zt1o1bcz	398	18	time	time	NN
cord-009318-zt1o1bcz	398	19	in	in	IN
cord-009318-zt1o1bcz	398	20	both	both	CC
cord-009318-zt1o1bcz	398	21	linear	linear	JJ
cord-009318-zt1o1bcz	398	22	(	(	-LRB-
cord-009318-zt1o1bcz	398	23	Figure	Figure	NNP
cord-009318-zt1o1bcz	398	24	7C	7C	NNP
cord-009318-zt1o1bcz	398	25	)	)	-RRB-
cord-009318-zt1o1bcz	398	26	and	and	CC
cord-009318-zt1o1bcz	398	27	logarithmic	logarithmic	JJ
cord-009318-zt1o1bcz	398	28	scales	scale	NNS
cord-009318-zt1o1bcz	399	1	(	(	-LRB-
cord-009318-zt1o1bcz	399	2	Figure	Figure	NNP
cord-009318-zt1o1bcz	399	3	7D	7D	NNP
cord-009318-zt1o1bcz	399	4	)	)	-RRB-
cord-009318-zt1o1bcz	399	5	.	.	.
cord-009318-zt1o1bcz	400	1	Assays	assay	NNS
cord-009318-zt1o1bcz	400	2	employing	employ	VBG
cord-009318-zt1o1bcz	400	3	HRM	HRM	NNP
cord-009318-zt1o1bcz	400	4	only	only	RB
cord-009318-zt1o1bcz	400	5	during	during	IN
cord-009318-zt1o1bcz	400	6	the	the	DT
cord-009318-zt1o1bcz	400	7	development	development	NN
cord-009318-zt1o1bcz	400	8	of	of	IN
cord-009318-zt1o1bcz	400	9	the	the	DT
cord-009318-zt1o1bcz	400	10	assay	assay	NN
cord-009318-zt1o1bcz	400	11	can	can	MD
cord-009318-zt1o1bcz	400	12	improve	improve	VB
cord-009318-zt1o1bcz	400	13	the	the	DT
cord-009318-zt1o1bcz	400	14	LOD	lod	NN
cord-009318-zt1o1bcz	400	15	of	of	IN
cord-009318-zt1o1bcz	400	16	the	the	DT
cord-009318-zt1o1bcz	400	17	final	final	JJ
cord-009318-zt1o1bcz	400	18	assays	assay	NNS
cord-009318-zt1o1bcz	400	19	by	by	IN
cord-009318-zt1o1bcz	400	20	selecting	select	VBG
cord-009318-zt1o1bcz	400	21	(	(	-LRB-
cord-009318-zt1o1bcz	400	22	making	make	VBG
cord-009318-zt1o1bcz	400	23	an	an	DT
cord-009318-zt1o1bcz	400	24	informed	informed	JJ
cord-009318-zt1o1bcz	400	25	choice	choice	NN
cord-009318-zt1o1bcz	400	26	of	of	IN
cord-009318-zt1o1bcz	400	27	the	the	DT
cord-009318-zt1o1bcz	400	28	optimum	optimum	JJ
cord-009318-zt1o1bcz	400	29	threshold	threshold	NN
cord-009318-zt1o1bcz	400	30	)	)	-RRB-
cord-009318-zt1o1bcz	400	31	.	.	.
cord-009318-zt1o1bcz	401	1	The	the	DT
cord-009318-zt1o1bcz	401	2	LOD	lod	NN
cord-009318-zt1o1bcz	401	3	decreases	decrease	VBZ
cord-009318-zt1o1bcz	401	4	(	(	-LRB-
cord-009318-zt1o1bcz	401	5	blue	blue	JJ
cord-009318-zt1o1bcz	401	6	curve	curve	NN
cord-009318-zt1o1bcz	401	7	)	)	-RRB-
cord-009318-zt1o1bcz	401	8	as	as	IN
cord-009318-zt1o1bcz	401	9	the	the	DT
cord-009318-zt1o1bcz	401	10	true	true	JJ
cord-009318-zt1o1bcz	401	11	positives	positive	NNS
cord-009318-zt1o1bcz	401	12	begin	begin	VBP
cord-009318-zt1o1bcz	401	13	to	to	TO
cord-009318-zt1o1bcz	401	14	amplify	amplify	VB
cord-009318-zt1o1bcz	401	15	(	(	-LRB-
cord-009318-zt1o1bcz	401	16	blue	blue	NNP
cord-009318-zt1o1bcz	401	17	dashed	dash	VBN
cord-009318-zt1o1bcz	401	18	)	)	-RRB-
cord-009318-zt1o1bcz	401	19	and	and	CC
cord-009318-zt1o1bcz	401	20	increases	increase	NNS
cord-009318-zt1o1bcz	401	21	,	,	,
cord-009318-zt1o1bcz	401	22	as	as	IN
cord-009318-zt1o1bcz	401	23	the	the	DT
cord-009318-zt1o1bcz	401	24	false	false	JJ
cord-009318-zt1o1bcz	401	25	positives	positive	NNS
cord-009318-zt1o1bcz	401	26	amplify	amplify	VBP
cord-009318-zt1o1bcz	401	27	(	(	-LRB-
cord-009318-zt1o1bcz	401	28	red	red	NNP
cord-009318-zt1o1bcz	401	29	dashed	dash	VBD
cord-009318-zt1o1bcz	401	30	)	)	-RRB-
cord-009318-zt1o1bcz	401	31	.	.	.
cord-009318-zt1o1bcz	402	1	The	the	DT
cord-009318-zt1o1bcz	402	2	minima	minima	NN
cord-009318-zt1o1bcz	402	3	for	for	IN
cord-009318-zt1o1bcz	402	4	this	this	DT
cord-009318-zt1o1bcz	402	5	system	system	NN
cord-009318-zt1o1bcz	402	6	occurs	occur	VBZ
cord-009318-zt1o1bcz	402	7	at	at	IN
cord-009318-zt1o1bcz	402	8	34	34	CD
cord-009318-zt1o1bcz	402	9	min	min	NN
cord-009318-zt1o1bcz	402	10	and	and	CC
cord-009318-zt1o1bcz	402	11	0.93	0.93	CD
cord-009318-zt1o1bcz	402	12	±	±	NNP
cord-009318-zt1o1bcz	402	13	16	16	CD
cord-009318-zt1o1bcz	402	14	cp	cp	NN
cord-009318-zt1o1bcz	402	15	/	/	SYM
cord-009318-zt1o1bcz	402	16	l	l	NN
cord-009318-zt1o1bcz	402	17	,	,	,
cord-009318-zt1o1bcz	402	18	striking	strike	VBG
cord-009318-zt1o1bcz	402	19	a	a	DT
cord-009318-zt1o1bcz	402	20	balance	balance	NN
cord-009318-zt1o1bcz	402	21	between	between	IN
cord-009318-zt1o1bcz	402	22	allowing	allow	VBG
cord-009318-zt1o1bcz	402	23	many	many	JJ
cord-009318-zt1o1bcz	402	24	true	true	JJ
cord-009318-zt1o1bcz	402	25	positives	positive	NNS
cord-009318-zt1o1bcz	402	26	to	to	TO
cord-009318-zt1o1bcz	402	27	amplify	amplify	VB
cord-009318-zt1o1bcz	402	28	and	and	CC
cord-009318-zt1o1bcz	402	29	only	only	RB
cord-009318-zt1o1bcz	402	30	a	a	DT
cord-009318-zt1o1bcz	402	31	small	small	JJ
cord-009318-zt1o1bcz	402	32	amount	amount	NN
cord-009318-zt1o1bcz	402	33	of	of	IN
cord-009318-zt1o1bcz	402	34	false	false	JJ
cord-009318-zt1o1bcz	402	35	positives	positive	NNS
cord-009318-zt1o1bcz	402	36	to	to	TO
cord-009318-zt1o1bcz	402	37	occur	occur	VB
cord-009318-zt1o1bcz	402	38	(	(	-LRB-
cord-009318-zt1o1bcz	402	39	53.6	53.6	CD
cord-009318-zt1o1bcz	402	40	%	%	NN
cord-009318-zt1o1bcz	402	41	true	true	JJ
cord-009318-zt1o1bcz	402	42	-	-	HYPH
cord-009318-zt1o1bcz	402	43	positive	positive	JJ
cord-009318-zt1o1bcz	402	44	fraction	fraction	NN
cord-009318-zt1o1bcz	402	45	and	and	CC
cord-009318-zt1o1bcz	402	46	1.5	1.5	CD
cord-009318-zt1o1bcz	402	47	%	%	NN
cord-009318-zt1o1bcz	402	48	false	false	JJ
cord-009318-zt1o1bcz	402	49	-	-	HYPH
cord-009318-zt1o1bcz	402	50	positive	positive	JJ
cord-009318-zt1o1bcz	402	51	fraction	fraction	NN
cord-009318-zt1o1bcz	402	52	)	)	-RRB-
cord-009318-zt1o1bcz	402	53	and	and	CC
cord-009318-zt1o1bcz	402	54	is	be	VBZ
cord-009318-zt1o1bcz	402	55	clearly	clearly	RB
cord-009318-zt1o1bcz	402	56	defined	define	VBN
cord-009318-zt1o1bcz	402	57	using	use	VBG
cord-009318-zt1o1bcz	402	58	the	the	DT
cord-009318-zt1o1bcz	402	59	linear	linear	JJ
cord-009318-zt1o1bcz	402	60	scale	scale	NN
cord-009318-zt1o1bcz	402	61	(	(	-LRB-
cord-009318-zt1o1bcz	402	62	Figure	Figure	NNP
cord-009318-zt1o1bcz	402	63	7C	7C	NNP
cord-009318-zt1o1bcz	402	64	)	)	-RRB-
cord-009318-zt1o1bcz	402	65	.	.	.
cord-009318-zt1o1bcz	403	1	Plotting	plotting	NN
cord-009318-zt1o1bcz	403	2	of	of	IN
cord-009318-zt1o1bcz	403	3	LOD	lod	NN
cord-009318-zt1o1bcz	403	4	on	on	IN
cord-009318-zt1o1bcz	403	5	the	the	DT
cord-009318-zt1o1bcz	403	6	logarithmic	logarithmic	JJ
cord-009318-zt1o1bcz	403	7	scale	scale	NN
cord-009318-zt1o1bcz	403	8	(	(	-LRB-
cord-009318-zt1o1bcz	403	9	Figure	figure	NN
cord-009318-zt1o1bcz	403	10	7D	7d	NN
cord-009318-zt1o1bcz	403	11	)	)	-RRB-
cord-009318-zt1o1bcz	403	12	emphasizes	emphasize	VBZ
cord-009318-zt1o1bcz	403	13	improperly	improperly	RB
cord-009318-zt1o1bcz	403	14	selecting	select	VBG
cord-009318-zt1o1bcz	403	15	a	a	DT
cord-009318-zt1o1bcz	403	16	threshold	threshold	NN
cord-009318-zt1o1bcz	403	17	can	can	MD
cord-009318-zt1o1bcz	403	18	result	result	VB
cord-009318-zt1o1bcz	403	19	in	in	IN
cord-009318-zt1o1bcz	403	20	several	several	JJ
cord-009318-zt1o1bcz	403	21	orders	order	NNS
cord-009318-zt1o1bcz	403	22	of	of	IN
cord-009318-zt1o1bcz	403	23	magnitude	magnitude	NN
cord-009318-zt1o1bcz	403	24	loss	loss	NN
cord-009318-zt1o1bcz	403	25	in	in	IN
cord-009318-zt1o1bcz	403	26	assay	assay	NN
cord-009318-zt1o1bcz	403	27	performance	performance	NN
cord-009318-zt1o1bcz	403	28	(	(	-LRB-
cord-009318-zt1o1bcz	403	29	for	for	IN
cord-009318-zt1o1bcz	403	30	example	example	NN
cord-009318-zt1o1bcz	403	31	,	,	,
cord-009318-zt1o1bcz	403	32	stopping	stop	VBG
cord-009318-zt1o1bcz	403	33	the	the	DT
cord-009318-zt1o1bcz	403	34	assay	assay	NN
cord-009318-zt1o1bcz	403	35	too	too	RB
cord-009318-zt1o1bcz	403	36	early	early	RB
cord-009318-zt1o1bcz	403	37	or	or	CC
cord-009318-zt1o1bcz	403	38	allowing	allow	VBG
cord-009318-zt1o1bcz	403	39	the	the	DT
cord-009318-zt1o1bcz	403	40	assay	assay	NN
cord-009318-zt1o1bcz	403	41	to	to	TO
cord-009318-zt1o1bcz	403	42	run	run	VB
cord-009318-zt1o1bcz	403	43	for	for	IN
cord-009318-zt1o1bcz	403	44	too	too	RB
cord-009318-zt1o1bcz	403	45	long	long	RB
cord-009318-zt1o1bcz	403	46	)	)	-RRB-
cord-009318-zt1o1bcz	403	47	.	.	.
cord-009318-zt1o1bcz	404	1	Although	although	IN
cord-009318-zt1o1bcz	404	2	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	404	3	is	be	VBZ
cord-009318-zt1o1bcz	404	4	robust	robust	JJ
cord-009318-zt1o1bcz	404	5	to	to	IN
cord-009318-zt1o1bcz	404	6	perturbations	perturbation	NNS
cord-009318-zt1o1bcz	404	7	,	,	,
cord-009318-zt1o1bcz	404	8	selecting	select	VBG
cord-009318-zt1o1bcz	404	9	the	the	DT
cord-009318-zt1o1bcz	404	10	appropriate	appropriate	JJ
cord-009318-zt1o1bcz	404	11	duration	duration	NN
cord-009318-zt1o1bcz	404	12	for	for	IN
cord-009318-zt1o1bcz	404	13	amplification	amplification	NN
cord-009318-zt1o1bcz	404	14	is	be	VBZ
cord-009318-zt1o1bcz	404	15	important	important	JJ
cord-009318-zt1o1bcz	404	16	.	.	.
cord-009318-zt1o1bcz	405	1	In	in	IN
cord-009318-zt1o1bcz	405	2	contrast	contrast	NN
cord-009318-zt1o1bcz	405	3	,	,	,
cord-009318-zt1o1bcz	405	4	assays	assay	NNS
cord-009318-zt1o1bcz	405	5	using	use	VBG
cord-009318-zt1o1bcz	405	6	HRM	HRM	NNP
cord-009318-zt1o1bcz	405	7	as	as	IN
cord-009318-zt1o1bcz	405	8	part	part	NN
cord-009318-zt1o1bcz	405	9	of	of	IN
cord-009318-zt1o1bcz	405	10	the	the	DT
cord-009318-zt1o1bcz	405	11	final	final	JJ
cord-009318-zt1o1bcz	405	12	readout	readout	NN
cord-009318-zt1o1bcz	405	13	can	can	MD
cord-009318-zt1o1bcz	405	14	distinguish	distinguish	VB
cord-009318-zt1o1bcz	405	15	false	false	JJ
cord-009318-zt1o1bcz	405	16	positives	positive	NNS
cord-009318-zt1o1bcz	405	17	from	from	IN
cord-009318-zt1o1bcz	405	18	the	the	DT
cord-009318-zt1o1bcz	405	19	true	true	JJ
cord-009318-zt1o1bcz	405	20	positives	positive	NNS
cord-009318-zt1o1bcz	405	21	and	and	CC
cord-009318-zt1o1bcz	405	22	improve	improve	VB
cord-009318-zt1o1bcz	405	23	LOD	lod	NN
cord-009318-zt1o1bcz	405	24	further	far	RBR
cord-009318-zt1o1bcz	405	25	by	by	IN
cord-009318-zt1o1bcz	405	26	excluding	exclude	VBG
cord-009318-zt1o1bcz	405	27	non	non	JJ
cord-009318-zt1o1bcz	405	28	-	-	JJ
cord-009318-zt1o1bcz	405	29	specific	specific	JJ
cord-009318-zt1o1bcz	405	30	amplification	amplification	NN
cord-009318-zt1o1bcz	405	31	from	from	IN
cord-009318-zt1o1bcz	405	32	the	the	DT
cord-009318-zt1o1bcz	405	33	analysis	analysis	NN
cord-009318-zt1o1bcz	405	34	.	.	.
cord-009318-zt1o1bcz	406	1	In	in	IN
cord-009318-zt1o1bcz	406	2	some	some	DT
cord-009318-zt1o1bcz	406	3	instances	instance	NNS
cord-009318-zt1o1bcz	406	4	,	,	,
cord-009318-zt1o1bcz	406	5	an	an	DT
cord-009318-zt1o1bcz	406	6	NTC	NTC	NNP
cord-009318-zt1o1bcz	406	7	may	may	MD
cord-009318-zt1o1bcz	406	8	incorrectly	incorrectly	RB
cord-009318-zt1o1bcz	406	9	identify	identify	VB
cord-009318-zt1o1bcz	406	10	partitions	partition	NNS
cord-009318-zt1o1bcz	406	11	as	as	IN
cord-009318-zt1o1bcz	406	12	true	true	JJ
cord-009318-zt1o1bcz	406	13	positives	positive	NNS
cord-009318-zt1o1bcz	406	14	by	by	IN
cord-009318-zt1o1bcz	406	15	T	T	NNP
cord-009318-zt1o1bcz	406	16	m	m	NNP
cord-009318-zt1o1bcz	406	17	(	(	-LRB-
cord-009318-zt1o1bcz	406	18	black	black	NNP
cord-009318-zt1o1bcz	406	19	dashed	dash	VBD
cord-009318-zt1o1bcz	406	20	)	)	-RRB-
cord-009318-zt1o1bcz	406	21	.	.	.
cord-009318-zt1o1bcz	407	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	407	2	incorporate	incorporate	VBP
cord-009318-zt1o1bcz	407	3	these	these	DT
cord-009318-zt1o1bcz	407	4	events	event	NNS
cord-009318-zt1o1bcz	407	5	as	as	IN
cord-009318-zt1o1bcz	407	6	non	non	JJ
cord-009318-zt1o1bcz	407	7	-	-	JJ
cord-009318-zt1o1bcz	407	8	specific	specific	JJ
cord-009318-zt1o1bcz	407	9	amplification	amplification	NN
cord-009318-zt1o1bcz	407	10	in	in	IN
cord-009318-zt1o1bcz	407	11	the	the	DT
cord-009318-zt1o1bcz	407	12	case	case	NN
cord-009318-zt1o1bcz	407	13	HRM	HRM	NNP
cord-009318-zt1o1bcz	407	14	is	be	VBZ
cord-009318-zt1o1bcz	407	15	used	use	VBN
cord-009318-zt1o1bcz	407	16	in	in	IN
cord-009318-zt1o1bcz	407	17	the	the	DT
cord-009318-zt1o1bcz	407	18	final	final	JJ
cord-009318-zt1o1bcz	407	19	readout	readout	NN
cord-009318-zt1o1bcz	407	20	.	.	.
cord-009318-zt1o1bcz	408	1	If	if	IN
cord-009318-zt1o1bcz	408	2	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	408	3	amplification	amplification	NN
cord-009318-zt1o1bcz	408	4	is	be	VBZ
cord-009318-zt1o1bcz	408	5	eliminated	eliminate	VBN
cord-009318-zt1o1bcz	408	6	,	,	,
cord-009318-zt1o1bcz	408	7	the	the	DT
cord-009318-zt1o1bcz	408	8	assay	assay	NN
cord-009318-zt1o1bcz	408	9	LOD	lod	NN
cord-009318-zt1o1bcz	408	10	(	(	-LRB-
cord-009318-zt1o1bcz	408	11	Figure	figure	NN
cord-009318-zt1o1bcz	408	12	 	 	_SP
cord-009318-zt1o1bcz	408	13	7C	7C	NNP
cord-009318-zt1o1bcz	408	14	and	and	CC
cord-009318-zt1o1bcz	408	15	F	F	NNP
cord-009318-zt1o1bcz	408	16	,	,	,
cord-009318-zt1o1bcz	408	17	black	black	NNP
cord-009318-zt1o1bcz	408	18	solid	solid	JJ
cord-009318-zt1o1bcz	408	19	)	)	-RRB-
cord-009318-zt1o1bcz	408	20	continues	continue	VBZ
cord-009318-zt1o1bcz	408	21	to	to	TO
cord-009318-zt1o1bcz	408	22	improve	improve	VB
cord-009318-zt1o1bcz	408	23	with	with	IN
cord-009318-zt1o1bcz	408	24	time	time	NN
cord-009318-zt1o1bcz	408	25	,	,	,
cord-009318-zt1o1bcz	408	26	and	and	CC
cord-009318-zt1o1bcz	408	27	is	be	VBZ
cord-009318-zt1o1bcz	408	28	only	only	RB
cord-009318-zt1o1bcz	408	29	dependent	dependent	JJ
cord-009318-zt1o1bcz	408	30	on	on	IN
cord-009318-zt1o1bcz	408	31	the	the	DT
cord-009318-zt1o1bcz	408	32	stochastic	stochastic	JJ
cord-009318-zt1o1bcz	408	33	probability	probability	NN
cord-009318-zt1o1bcz	408	34	that	that	IN
cord-009318-zt1o1bcz	408	35	a	a	DT
cord-009318-zt1o1bcz	408	36	true	true	JJ
cord-009318-zt1o1bcz	408	37	positive	positive	JJ
cord-009318-zt1o1bcz	408	38	will	will	MD
cord-009318-zt1o1bcz	408	39	initiate	initiate	VB
cord-009318-zt1o1bcz	408	40	and	and	CC
cord-009318-zt1o1bcz	408	41	amplify	amplify	VB
cord-009318-zt1o1bcz	408	42	.	.	.
cord-009318-zt1o1bcz	409	1	In	in	IN
cord-009318-zt1o1bcz	409	2	this	this	DT
cord-009318-zt1o1bcz	409	3	scenario	scenario	NN
cord-009318-zt1o1bcz	409	4	,	,	,
cord-009318-zt1o1bcz	409	5	there	there	EX
cord-009318-zt1o1bcz	409	6	is	be	VBZ
cord-009318-zt1o1bcz	409	7	no	no	DT
cord-009318-zt1o1bcz	409	8	penalty	penalty	NN
cord-009318-zt1o1bcz	409	9	allowing	allow	VBG
cord-009318-zt1o1bcz	409	10	the	the	DT
cord-009318-zt1o1bcz	409	11	assay	assay	NN
cord-009318-zt1o1bcz	409	12	to	to	TO
cord-009318-zt1o1bcz	409	13	amplify	amplify	VB
cord-009318-zt1o1bcz	409	14	for	for	IN
cord-009318-zt1o1bcz	409	15	extended	extended	JJ
cord-009318-zt1o1bcz	409	16	periods	period	NNS
cord-009318-zt1o1bcz	409	17	of	of	IN
cord-009318-zt1o1bcz	409	18	time	time	NN
cord-009318-zt1o1bcz	409	19	.	.	.
cord-009318-zt1o1bcz	410	1	In	in	IN
cord-009318-zt1o1bcz	410	2	this	this	DT
cord-009318-zt1o1bcz	410	3	scenario	scenario	NN
cord-009318-zt1o1bcz	410	4	the	the	DT
cord-009318-zt1o1bcz	410	5	LOD	lod	NN
cord-009318-zt1o1bcz	410	6	equation	equation	NN
cord-009318-zt1o1bcz	410	7	simplifies	simplify	VBZ
cord-009318-zt1o1bcz	410	8	to	to	IN
cord-009318-zt1o1bcz	410	9	Additionally	additionally	RB
cord-009318-zt1o1bcz	410	10	,	,	,
cord-009318-zt1o1bcz	410	11	there	there	EX
cord-009318-zt1o1bcz	410	12	is	be	VBZ
cord-009318-zt1o1bcz	410	13	no	no	DT
cord-009318-zt1o1bcz	410	14	limitation	limitation	NN
cord-009318-zt1o1bcz	410	15	on	on	IN
cord-009318-zt1o1bcz	410	16	the	the	DT
cord-009318-zt1o1bcz	410	17	number	number	NN
cord-009318-zt1o1bcz	410	18	of	of	IN
cord-009318-zt1o1bcz	410	19	parameters	parameter	NNS
cord-009318-zt1o1bcz	410	20	that	that	WDT
cord-009318-zt1o1bcz	410	21	can	can	MD
cord-009318-zt1o1bcz	410	22	be	be	VB
cord-009318-zt1o1bcz	410	23	used	use	VBN
cord-009318-zt1o1bcz	410	24	to	to	TO
cord-009318-zt1o1bcz	410	25	identify	identify	VB
cord-009318-zt1o1bcz	410	26	the	the	DT
cord-009318-zt1o1bcz	410	27	optimal	optimal	JJ
cord-009318-zt1o1bcz	410	28	LOD	lod	NN
cord-009318-zt1o1bcz	410	29	.	.	.
cord-009318-zt1o1bcz	411	1	Using	use	VBG
cord-009318-zt1o1bcz	411	2	multiple	multiple	JJ
cord-009318-zt1o1bcz	411	3	parameters	parameter	NNS
cord-009318-zt1o1bcz	411	4	to	to	TO
cord-009318-zt1o1bcz	411	5	filter	filter	VB
cord-009318-zt1o1bcz	411	6	the	the	DT
cord-009318-zt1o1bcz	411	7	data	datum	NNS
cord-009318-zt1o1bcz	411	8	may	may	MD
cord-009318-zt1o1bcz	411	9	be	be	VB
cord-009318-zt1o1bcz	411	10	useful	useful	JJ
cord-009318-zt1o1bcz	411	11	for	for	IN
cord-009318-zt1o1bcz	411	12	individuals	individual	NNS
cord-009318-zt1o1bcz	411	13	not	not	RB
cord-009318-zt1o1bcz	411	14	employing	employ	VBG
cord-009318-zt1o1bcz	411	15	HRM	HRM	NNP
cord-009318-zt1o1bcz	411	16	in	in	IN
cord-009318-zt1o1bcz	411	17	the	the	DT
cord-009318-zt1o1bcz	411	18	final	final	JJ
cord-009318-zt1o1bcz	411	19	assay	assay	NN
cord-009318-zt1o1bcz	411	20	or	or	CC
cord-009318-zt1o1bcz	411	21	in	in	IN
cord-009318-zt1o1bcz	411	22	assays	assay	NNS
cord-009318-zt1o1bcz	411	23	only	only	RB
cord-009318-zt1o1bcz	411	24	employing	employ	VBG
cord-009318-zt1o1bcz	411	25	end	end	NN
cord-009318-zt1o1bcz	411	26	-	-	HYPH
cord-009318-zt1o1bcz	411	27	point	point	NN
cord-009318-zt1o1bcz	411	28	measurement	measurement	NN
cord-009318-zt1o1bcz	411	29	(	(	-LRB-
cord-009318-zt1o1bcz	412	1	e.g.	e.g.	RB
cord-009318-zt1o1bcz	412	2	an	an	DT
cord-009318-zt1o1bcz	412	3	assay	assay	NN
cord-009318-zt1o1bcz	412	4	without	without	IN
cord-009318-zt1o1bcz	412	5	real	real	JJ
cord-009318-zt1o1bcz	412	6	-	-	HYPH
cord-009318-zt1o1bcz	412	7	time	time	NN
cord-009318-zt1o1bcz	412	8	measurements	measurement	NNS
cord-009318-zt1o1bcz	412	9	will	will	MD
cord-009318-zt1o1bcz	412	10	be	be	VB
cord-009318-zt1o1bcz	412	11	unable	unable	JJ
cord-009318-zt1o1bcz	412	12	to	to	TO
cord-009318-zt1o1bcz	412	13	generate	generate	VB
cord-009318-zt1o1bcz	412	14	data	datum	NNS
cord-009318-zt1o1bcz	412	15	on	on	IN
cord-009318-zt1o1bcz	412	16	rate	rate	NN
cord-009318-zt1o1bcz	412	17	,	,	,
cord-009318-zt1o1bcz	412	18	but	but	CC
cord-009318-zt1o1bcz	412	19	still	still	RB
cord-009318-zt1o1bcz	412	20	benefit	benefit	VBP
cord-009318-zt1o1bcz	412	21	from	from	IN
cord-009318-zt1o1bcz	412	22	selecting	select	VBG
cord-009318-zt1o1bcz	412	23	optimal	optimal	JJ
cord-009318-zt1o1bcz	412	24	assay	assay	NN
cord-009318-zt1o1bcz	412	25	time	time	NN
cord-009318-zt1o1bcz	412	26	and	and	CC
cord-009318-zt1o1bcz	412	27	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	412	28	threshold	threshold	NN
cord-009318-zt1o1bcz	412	29	)	)	-RRB-
cord-009318-zt1o1bcz	412	30	.	.	.
cord-009318-zt1o1bcz	413	1	As	as	IN
cord-009318-zt1o1bcz	413	2	a	a	DT
cord-009318-zt1o1bcz	413	3	demonstration	demonstration	NN
cord-009318-zt1o1bcz	413	4	,	,	,
cord-009318-zt1o1bcz	413	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	413	6	filtered	filter	VBD
cord-009318-zt1o1bcz	413	7	first	first	RB
cord-009318-zt1o1bcz	413	8	by	by	IN
cord-009318-zt1o1bcz	413	9	optimal	optimal	JJ
cord-009318-zt1o1bcz	413	10	TTP	TTP	NNP
cord-009318-zt1o1bcz	413	11	,	,	,
cord-009318-zt1o1bcz	413	12	then	then	RB
cord-009318-zt1o1bcz	413	13	for	for	IN
cord-009318-zt1o1bcz	413	14	the	the	DT
cord-009318-zt1o1bcz	413	15	optima	optima	NNP
cord-009318-zt1o1bcz	413	16	of	of	IN
cord-009318-zt1o1bcz	413	17	a	a	DT
cord-009318-zt1o1bcz	413	18	second	second	JJ
cord-009318-zt1o1bcz	413	19	parameter	parameter	NN
cord-009318-zt1o1bcz	413	20	.	.	.
cord-009318-zt1o1bcz	414	1	In	in	IN
cord-009318-zt1o1bcz	414	2	this	this	DT
cord-009318-zt1o1bcz	414	3	case	case	NN
cord-009318-zt1o1bcz	414	4	,	,	,
cord-009318-zt1o1bcz	414	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	414	6	selected	select	VBD
cord-009318-zt1o1bcz	414	7	the	the	DT
cord-009318-zt1o1bcz	414	8	optimal	optimal	JJ
cord-009318-zt1o1bcz	414	9	TTP	ttp	NN
cord-009318-zt1o1bcz	414	10	of	of	IN
cord-009318-zt1o1bcz	414	11	34	34	CD
cord-009318-zt1o1bcz	414	12	min	min	NN
cord-009318-zt1o1bcz	414	13	,	,	,
cord-009318-zt1o1bcz	414	14	and	and	CC
cord-009318-zt1o1bcz	414	15	scanned	scan	VBD
cord-009318-zt1o1bcz	414	16	for	for	IN
cord-009318-zt1o1bcz	414	17	optimal	optimal	JJ
cord-009318-zt1o1bcz	414	18	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	414	19	threshold	threshold	NN
cord-009318-zt1o1bcz	414	20	.	.	.
cord-009318-zt1o1bcz	415	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	415	2	plotted	plot	VBD
cord-009318-zt1o1bcz	415	3	LOD	LOD	NNP
cord-009318-zt1o1bcz	415	4	as	as	IN
cord-009318-zt1o1bcz	415	5	a	a	DT
cord-009318-zt1o1bcz	415	6	function	function	NN
cord-009318-zt1o1bcz	415	7	of	of	IN
cord-009318-zt1o1bcz	415	8	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	415	9	threshold	threshold	NN
cord-009318-zt1o1bcz	415	10	and	and	CC
cord-009318-zt1o1bcz	415	11	determined	determine	VBD
cord-009318-zt1o1bcz	415	12	that	that	IN
cord-009318-zt1o1bcz	415	13	the	the	DT
cord-009318-zt1o1bcz	415	14	optimal	optimal	JJ
cord-009318-zt1o1bcz	415	15	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	415	16	threshold	threshold	NN
cord-009318-zt1o1bcz	415	17	at	at	IN
cord-009318-zt1o1bcz	415	18	34	34	CD
cord-009318-zt1o1bcz	415	19	min	min	NN
cord-009318-zt1o1bcz	415	20	would	would	MD
cord-009318-zt1o1bcz	415	21	be	be	VB
cord-009318-zt1o1bcz	415	22	248	248	CD
cord-009318-zt1o1bcz	415	23	RFU	rfu	NN
cord-009318-zt1o1bcz	415	24	and	and	CC
cord-009318-zt1o1bcz	415	25	correspond	correspond	VB
cord-009318-zt1o1bcz	415	26	to	to	IN
cord-009318-zt1o1bcz	415	27	an	an	DT
cord-009318-zt1o1bcz	415	28	LOD	lod	NN
cord-009318-zt1o1bcz	415	29	of	of	IN
cord-009318-zt1o1bcz	415	30	0.97	0.97	CD
cord-009318-zt1o1bcz	415	31	±	±	CD
cord-009318-zt1o1bcz	415	32	0.16	0.16	CD
cord-009318-zt1o1bcz	415	33	cp	cp	NN
cord-009318-zt1o1bcz	415	34	/	/	SYM
cord-009318-zt1o1bcz	415	35	l	l	NN
cord-009318-zt1o1bcz	415	36	(	(	-LRB-
cord-009318-zt1o1bcz	415	37	Figure	Figure	NNP
cord-009318-zt1o1bcz	415	38	7E	7E	NNP
cord-009318-zt1o1bcz	415	39	)	)	-RRB-
cord-009318-zt1o1bcz	415	40	.	.	.
cord-009318-zt1o1bcz	416	1	Do	do	VB
cord-009318-zt1o1bcz	416	2	filter	filter	NN
cord-009318-zt1o1bcz	416	3	parameters	parameter	NNS
cord-009318-zt1o1bcz	416	4	exhibit	exhibit	VB
cord-009318-zt1o1bcz	416	5	the	the	DT
cord-009318-zt1o1bcz	416	6	same	same	JJ
cord-009318-zt1o1bcz	416	7	LOD	lod	NN
cord-009318-zt1o1bcz	416	8	minima	minima	NN
cord-009318-zt1o1bcz	416	9	when	when	WRB
cord-009318-zt1o1bcz	416	10	using	use	VBG
cord-009318-zt1o1bcz	416	11	Bst	Bst	NNP
cord-009318-zt1o1bcz	416	12	2.0	2.0	CD
cord-009318-zt1o1bcz	416	13	,	,	,
cord-009318-zt1o1bcz	416	14	as	as	IN
cord-009318-zt1o1bcz	416	15	they	-PRON-	PRP
cord-009318-zt1o1bcz	416	16	did	do	VBD
cord-009318-zt1o1bcz	416	17	for	for	IN
cord-009318-zt1o1bcz	416	18	Bst	Bst	NNP
cord-009318-zt1o1bcz	416	19	3.0	3.0	NNP
cord-009318-zt1o1bcz	416	20	?	?	.
cord-009318-zt1o1bcz	417	1	Bst	Bst	NNP
cord-009318-zt1o1bcz	417	2	2.0	2.0	CD
cord-009318-zt1o1bcz	417	3	had	have	VBD
cord-009318-zt1o1bcz	417	4	much	much	RB
cord-009318-zt1o1bcz	417	5	lower	low	JJR
cord-009318-zt1o1bcz	417	6	non	non	JJ
cord-009318-zt1o1bcz	417	7	-	-	JJ
cord-009318-zt1o1bcz	417	8	specific	specific	JJ
cord-009318-zt1o1bcz	417	9	background	background	NN
cord-009318-zt1o1bcz	417	10	than	than	IN
cord-009318-zt1o1bcz	417	11	Bst	Bst	NNP
cord-009318-zt1o1bcz	417	12	3.0	3.0	NNP
cord-009318-zt1o1bcz	417	13	,	,	,
cord-009318-zt1o1bcz	417	14	and	and	CC
cord-009318-zt1o1bcz	417	15	could	could	MD
cord-009318-zt1o1bcz	417	16	behave	behave	VB
cord-009318-zt1o1bcz	417	17	similarly	similarly	RB
cord-009318-zt1o1bcz	417	18	or	or	CC
cord-009318-zt1o1bcz	417	19	may	may	MD
cord-009318-zt1o1bcz	417	20	behave	behave	VB
cord-009318-zt1o1bcz	417	21	differently	differently	RB
cord-009318-zt1o1bcz	417	22	.	.	.
cord-009318-zt1o1bcz	418	1	First	first	RB
cord-009318-zt1o1bcz	418	2	,	,	,
cord-009318-zt1o1bcz	418	3	does	do	VBZ
cord-009318-zt1o1bcz	418	4	the	the	DT
cord-009318-zt1o1bcz	418	5	ROC	ROC	NNP
cord-009318-zt1o1bcz	418	6	curve	curve	NN
cord-009318-zt1o1bcz	418	7	for	for	IN
cord-009318-zt1o1bcz	418	8	TTP	TTP	NNP
cord-009318-zt1o1bcz	418	9	display	display	NN
cord-009318-zt1o1bcz	418	10	a	a	DT
cord-009318-zt1o1bcz	418	11	clear	clear	JJ
cord-009318-zt1o1bcz	418	12	optimum	optimum	NN
cord-009318-zt1o1bcz	418	13	?	?	.
cord-009318-zt1o1bcz	419	1	Similar	similar	JJ
cord-009318-zt1o1bcz	419	2	to	to	IN
cord-009318-zt1o1bcz	419	3	the	the	DT
cord-009318-zt1o1bcz	419	4	TTP	TTP	NNP
cord-009318-zt1o1bcz	419	5	ROC	ROC	NNP
cord-009318-zt1o1bcz	419	6	for	for	IN
cord-009318-zt1o1bcz	419	7	Bst	Bst	NNP
cord-009318-zt1o1bcz	419	8	3.0	3.0	NNP
cord-009318-zt1o1bcz	419	9	(	(	-LRB-
cord-009318-zt1o1bcz	419	10	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	419	11	Figure	Figure	NNP
cord-009318-zt1o1bcz	419	12	S9E	S9E	NNP
cord-009318-zt1o1bcz	419	13	)	)	-RRB-
cord-009318-zt1o1bcz	419	14	,	,	,
cord-009318-zt1o1bcz	419	15	the	the	DT
cord-009318-zt1o1bcz	419	16	TTP	TTP	NNP
cord-009318-zt1o1bcz	419	17	ROC	ROC	NNP
cord-009318-zt1o1bcz	419	18	for	for	IN
cord-009318-zt1o1bcz	419	19	Bst	Bst	NNP
cord-009318-zt1o1bcz	419	20	2.0	2.0	CD
cord-009318-zt1o1bcz	419	21	has	have	VBZ
cord-009318-zt1o1bcz	419	22	a	a	DT
cord-009318-zt1o1bcz	419	23	concave	concave	JJ
cord-009318-zt1o1bcz	419	24	slope	slope	NN
cord-009318-zt1o1bcz	419	25	making	make	VBG
cord-009318-zt1o1bcz	419	26	choice	choice	NN
cord-009318-zt1o1bcz	419	27	of	of	IN
cord-009318-zt1o1bcz	419	28	the	the	DT
cord-009318-zt1o1bcz	419	29	optimum	optimum	JJ
cord-009318-zt1o1bcz	419	30	a	a	DT
cord-009318-zt1o1bcz	419	31	matter	matter	NN
cord-009318-zt1o1bcz	419	32	of	of	IN
cord-009318-zt1o1bcz	419	33	computation	computation	NN
cord-009318-zt1o1bcz	419	34	(	(	-LRB-
cord-009318-zt1o1bcz	419	35	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	419	36	Figure	Figure	NNP
cord-009318-zt1o1bcz	419	37	S9F	s9f	NN
cord-009318-zt1o1bcz	419	38	)	)	-RRB-
cord-009318-zt1o1bcz	419	39	.	.	.
cord-009318-zt1o1bcz	420	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	420	2	can	can	MD
cord-009318-zt1o1bcz	420	3	visually	visually	RB
cord-009318-zt1o1bcz	420	4	estimate	estimate	VB
cord-009318-zt1o1bcz	420	5	the	the	DT
cord-009318-zt1o1bcz	420	6	balance	balance	NN
cord-009318-zt1o1bcz	420	7	of	of	IN
cord-009318-zt1o1bcz	420	8	true	true	JJ
cord-009318-zt1o1bcz	420	9	and	and	CC
cord-009318-zt1o1bcz	420	10	false	false	JJ
cord-009318-zt1o1bcz	420	11	-	-	HYPH
cord-009318-zt1o1bcz	420	12	positive	positive	JJ
cord-009318-zt1o1bcz	420	13	fraction	fraction	NN
cord-009318-zt1o1bcz	420	14	in	in	IN
cord-009318-zt1o1bcz	420	15	the	the	DT
cord-009318-zt1o1bcz	420	16	range	range	NN
cord-009318-zt1o1bcz	420	17	of	of	IN
cord-009318-zt1o1bcz	420	18	50	50	CD
cord-009318-zt1o1bcz	420	19	%	%	NN
cord-009318-zt1o1bcz	420	20	true	true	JJ
cord-009318-zt1o1bcz	420	21	and	and	CC
cord-009318-zt1o1bcz	420	22	10	10	CD
cord-009318-zt1o1bcz	420	23	%	%	NN
cord-009318-zt1o1bcz	420	24	false	false	JJ
cord-009318-zt1o1bcz	420	25	.	.	.
cord-009318-zt1o1bcz	421	1	Similar	similar	JJ
cord-009318-zt1o1bcz	421	2	curves	curve	NNS
cord-009318-zt1o1bcz	421	3	for	for	IN
cord-009318-zt1o1bcz	421	4	max	max	NN
cord-009318-zt1o1bcz	421	5	rate	rate	NN
cord-009318-zt1o1bcz	421	6	and	and	CC
cord-009318-zt1o1bcz	421	7	final	final	JJ
cord-009318-zt1o1bcz	421	8	intensity	intensity	NN
cord-009318-zt1o1bcz	421	9	could	could	MD
cord-009318-zt1o1bcz	421	10	be	be	VB
cord-009318-zt1o1bcz	421	11	generated	generate	VBN
cord-009318-zt1o1bcz	421	12	but	but	CC
cord-009318-zt1o1bcz	421	13	are	be	VBP
cord-009318-zt1o1bcz	421	14	not	not	RB
cord-009318-zt1o1bcz	421	15	shown	show	VBN
cord-009318-zt1o1bcz	421	16	here	here	RB
cord-009318-zt1o1bcz	421	17	.	.	.
cord-009318-zt1o1bcz	422	1	Second	second	RB
cord-009318-zt1o1bcz	422	2	,	,	,
cord-009318-zt1o1bcz	422	3	is	be	VBZ
cord-009318-zt1o1bcz	422	4	there	there	EX
cord-009318-zt1o1bcz	422	5	an	an	DT
cord-009318-zt1o1bcz	422	6	advantage	advantage	NN
cord-009318-zt1o1bcz	422	7	to	to	IN
cord-009318-zt1o1bcz	422	8	using	use	VBG
cord-009318-zt1o1bcz	422	9	HRM	HRM	NNP
cord-009318-zt1o1bcz	422	10	in	in	IN
cord-009318-zt1o1bcz	422	11	the	the	DT
cord-009318-zt1o1bcz	422	12	final	final	JJ
cord-009318-zt1o1bcz	422	13	assay	assay	NN
cord-009318-zt1o1bcz	422	14	with	with	IN
cord-009318-zt1o1bcz	422	15	Bst	Bst	NNP
cord-009318-zt1o1bcz	422	16	2.0	2.0	NNP
cord-009318-zt1o1bcz	422	17	?	?	.
cord-009318-zt1o1bcz	423	1	To	to	TO
cord-009318-zt1o1bcz	423	2	answer	answer	VB
cord-009318-zt1o1bcz	423	3	this	this	DT
cord-009318-zt1o1bcz	423	4	question	question	NN
cord-009318-zt1o1bcz	423	5	,	,	,
cord-009318-zt1o1bcz	423	6	we	-PRON-	PRP
cord-009318-zt1o1bcz	423	7	plot	plot	VBP
cord-009318-zt1o1bcz	423	8	LOD	lod	NN
cord-009318-zt1o1bcz	423	9	and	and	CC
cord-009318-zt1o1bcz	423	10	the	the	DT
cord-009318-zt1o1bcz	423	11	cumulative	cumulative	JJ
cord-009318-zt1o1bcz	423	12	counts	count	NNS
cord-009318-zt1o1bcz	423	13	of	of	IN
cord-009318-zt1o1bcz	423	14	true	true	JJ
cord-009318-zt1o1bcz	423	15	and	and	CC
cord-009318-zt1o1bcz	423	16	false	false	JJ
cord-009318-zt1o1bcz	423	17	positives	positive	NNS
cord-009318-zt1o1bcz	423	18	as	as	IN
cord-009318-zt1o1bcz	423	19	a	a	DT
cord-009318-zt1o1bcz	423	20	function	function	NN
cord-009318-zt1o1bcz	423	21	of	of	IN
cord-009318-zt1o1bcz	423	22	time	time	NN
cord-009318-zt1o1bcz	423	23	for	for	IN
cord-009318-zt1o1bcz	423	24	Bst	Bst	NNP
cord-009318-zt1o1bcz	423	25	2.0	2.0	CD
cord-009318-zt1o1bcz	423	26	(	(	-LRB-
cord-009318-zt1o1bcz	423	27	Figure	figure	NN
cord-009318-zt1o1bcz	423	28	7F	7f	NN
cord-009318-zt1o1bcz	423	29	)	)	-RRB-
cord-009318-zt1o1bcz	423	30	.	.	.
cord-009318-zt1o1bcz	424	1	Similarly	similarly	RB
cord-009318-zt1o1bcz	424	2	to	to	IN
cord-009318-zt1o1bcz	424	3	Bst	Bst	NNP
cord-009318-zt1o1bcz	424	4	3.0	3.0	NNP
cord-009318-zt1o1bcz	424	5	,	,	,
cord-009318-zt1o1bcz	424	6	we	-PRON-	PRP
cord-009318-zt1o1bcz	424	7	observe	observe	VBP
cord-009318-zt1o1bcz	424	8	LOD	LOD	NNP
cord-009318-zt1o1bcz	424	9	improve	improve	VB
cord-009318-zt1o1bcz	424	10	rapidly	rapidly	RB
cord-009318-zt1o1bcz	424	11	as	as	IN
cord-009318-zt1o1bcz	424	12	true	true	JJ
cord-009318-zt1o1bcz	424	13	-	-	HYPH
cord-009318-zt1o1bcz	424	14	positive	positive	JJ
cord-009318-zt1o1bcz	424	15	events	event	NNS
cord-009318-zt1o1bcz	424	16	are	be	VBP
cord-009318-zt1o1bcz	424	17	counted	count	VBN
cord-009318-zt1o1bcz	424	18	.	.	.
cord-009318-zt1o1bcz	425	1	However	however	RB
cord-009318-zt1o1bcz	425	2	,	,	,
cord-009318-zt1o1bcz	425	3	unlike	unlike	IN
cord-009318-zt1o1bcz	425	4	Bst	Bst	NNP
cord-009318-zt1o1bcz	425	5	3.0	3.0	NNP
cord-009318-zt1o1bcz	425	6	,	,	,
cord-009318-zt1o1bcz	425	7	the	the	DT
cord-009318-zt1o1bcz	425	8	non	non	JJ
cord-009318-zt1o1bcz	425	9	-	-	JJ
cord-009318-zt1o1bcz	425	10	specific	specific	JJ
cord-009318-zt1o1bcz	425	11	amplification	amplification	NN
cord-009318-zt1o1bcz	425	12	events	event	NNS
cord-009318-zt1o1bcz	425	13	are	be	VBP
cord-009318-zt1o1bcz	425	14	few	few	JJ
cord-009318-zt1o1bcz	425	15	and	and	CC
cord-009318-zt1o1bcz	425	16	their	-PRON-	PRP$
cord-009318-zt1o1bcz	425	17	presence	presence	NN
cord-009318-zt1o1bcz	425	18	does	do	VBZ
cord-009318-zt1o1bcz	425	19	not	not	RB
cord-009318-zt1o1bcz	425	20	have	have	VB
cord-009318-zt1o1bcz	425	21	an	an	DT
cord-009318-zt1o1bcz	425	22	impact	impact	NN
cord-009318-zt1o1bcz	425	23	on	on	IN
cord-009318-zt1o1bcz	425	24	LOD	LOD	NNP
cord-009318-zt1o1bcz	425	25	.	.	.
cord-009318-zt1o1bcz	426	1	Thus	thus	RB
cord-009318-zt1o1bcz	426	2	,	,	,
cord-009318-zt1o1bcz	426	3	when	when	WRB
cord-009318-zt1o1bcz	426	4	using	use	VBG
cord-009318-zt1o1bcz	426	5	Bst	Bst	NNP
cord-009318-zt1o1bcz	426	6	2.0	2.0	CD
cord-009318-zt1o1bcz	426	7	,	,	,
cord-009318-zt1o1bcz	426	8	the	the	DT
cord-009318-zt1o1bcz	426	9	curves	curve	NNS
cord-009318-zt1o1bcz	426	10	representing	represent	VBG
cord-009318-zt1o1bcz	426	11	LOD	lod	NN
cord-009318-zt1o1bcz	426	12	with	with	IN
cord-009318-zt1o1bcz	426	13	or	or	CC
cord-009318-zt1o1bcz	426	14	without	without	IN
cord-009318-zt1o1bcz	426	15	HRM	HRM	NNP
cord-009318-zt1o1bcz	426	16	in	in	IN
cord-009318-zt1o1bcz	426	17	the	the	DT
cord-009318-zt1o1bcz	426	18	final	final	JJ
cord-009318-zt1o1bcz	426	19	assay	assay	NN
cord-009318-zt1o1bcz	426	20	overlay	overlay	NN
cord-009318-zt1o1bcz	426	21	and	and	CC
cord-009318-zt1o1bcz	426	22	indicate	indicate	VBP
cord-009318-zt1o1bcz	426	23	using	use	VBG
cord-009318-zt1o1bcz	426	24	HRM	HRM	NNP
cord-009318-zt1o1bcz	426	25	in	in	IN
cord-009318-zt1o1bcz	426	26	the	the	DT
cord-009318-zt1o1bcz	426	27	final	final	JJ
cord-009318-zt1o1bcz	426	28	assay	assay	NN
cord-009318-zt1o1bcz	426	29	has	have	VBZ
cord-009318-zt1o1bcz	426	30	no	no	DT
cord-009318-zt1o1bcz	426	31	additional	additional	JJ
cord-009318-zt1o1bcz	426	32	benefit	benefit	NN
cord-009318-zt1o1bcz	426	33	.	.	.
cord-009318-zt1o1bcz	427	1	Furthermore	furthermore	RB
cord-009318-zt1o1bcz	427	2	,	,	,
cord-009318-zt1o1bcz	427	3	the	the	DT
cord-009318-zt1o1bcz	427	4	continuously	continuously	RB
cord-009318-zt1o1bcz	427	5	decreasing	decrease	VBG
cord-009318-zt1o1bcz	427	6	LOD	lod	NN
cord-009318-zt1o1bcz	427	7	with	with	IN
cord-009318-zt1o1bcz	427	8	time	time	NN
cord-009318-zt1o1bcz	427	9	for	for	IN
cord-009318-zt1o1bcz	427	10	either	either	DT
cord-009318-zt1o1bcz	427	11	case	case	NN
cord-009318-zt1o1bcz	427	12	indicates	indicate	VBZ
cord-009318-zt1o1bcz	427	13	that	that	IN
cord-009318-zt1o1bcz	427	14	use	use	NN
cord-009318-zt1o1bcz	427	15	of	of	IN
cord-009318-zt1o1bcz	427	16	ROC	ROC	NNP
cord-009318-zt1o1bcz	427	17	curves	curve	NNS
cord-009318-zt1o1bcz	427	18	to	to	TO
cord-009318-zt1o1bcz	427	19	determine	determine	VB
cord-009318-zt1o1bcz	427	20	an	an	DT
cord-009318-zt1o1bcz	427	21	optimum	optimum	NN
cord-009318-zt1o1bcz	427	22	can	can	MD
cord-009318-zt1o1bcz	427	23	be	be	VB
cord-009318-zt1o1bcz	427	24	misleading	misleading	JJ
cord-009318-zt1o1bcz	427	25	.	.	.
cord-009318-zt1o1bcz	428	1	While	while	IN
cord-009318-zt1o1bcz	428	2	the	the	DT
cord-009318-zt1o1bcz	428	3	ROC	ROC	NNP
cord-009318-zt1o1bcz	428	4	implies	imply	VBZ
cord-009318-zt1o1bcz	428	5	that	that	IN
cord-009318-zt1o1bcz	428	6	an	an	DT
cord-009318-zt1o1bcz	428	7	optimum	optimum	JJ
cord-009318-zt1o1bcz	428	8	exists	exist	VBZ
cord-009318-zt1o1bcz	428	9	,	,	,
cord-009318-zt1o1bcz	428	10	the	the	DT
cord-009318-zt1o1bcz	428	11	false	false	JJ
cord-009318-zt1o1bcz	428	12	-	-	HYPH
cord-009318-zt1o1bcz	428	13	positive	positive	JJ
cord-009318-zt1o1bcz	428	14	incidence	incidence	NN
cord-009318-zt1o1bcz	428	15	is	be	VBZ
cord-009318-zt1o1bcz	428	16	rare	rare	JJ
cord-009318-zt1o1bcz	428	17	enough	enough	RB
cord-009318-zt1o1bcz	428	18	that	that	IN
cord-009318-zt1o1bcz	428	19	a	a	DT
cord-009318-zt1o1bcz	428	20	TTP	TTP	NNP
cord-009318-zt1o1bcz	428	21	optimum	optimum	NN
cord-009318-zt1o1bcz	428	22	selected	select	VBN
cord-009318-zt1o1bcz	428	23	by	by	IN
cord-009318-zt1o1bcz	428	24	LOD	LOD	NNP
cord-009318-zt1o1bcz	428	25	does	do	VBZ
cord-009318-zt1o1bcz	428	26	not	not	RB
cord-009318-zt1o1bcz	428	27	exist	exist	VB
cord-009318-zt1o1bcz	428	28	.	.	.
cord-009318-zt1o1bcz	429	1	Hence	hence	RB
cord-009318-zt1o1bcz	429	2	,	,	,
cord-009318-zt1o1bcz	429	3	assay	assay	NN
cord-009318-zt1o1bcz	429	4	developers	developer	NNS
cord-009318-zt1o1bcz	429	5	may	may	MD
cord-009318-zt1o1bcz	429	6	select	select	VB
cord-009318-zt1o1bcz	429	7	assay	assay	NN
cord-009318-zt1o1bcz	429	8	time	time	NN
cord-009318-zt1o1bcz	429	9	based	base	VBN
cord-009318-zt1o1bcz	429	10	on	on	IN
cord-009318-zt1o1bcz	429	11	requirements	requirement	NNS
cord-009318-zt1o1bcz	429	12	other	other	JJ
cord-009318-zt1o1bcz	429	13	than	than	IN
cord-009318-zt1o1bcz	429	14	LOD	LOD	NNP
cord-009318-zt1o1bcz	429	15	.	.	.
cord-009318-zt1o1bcz	430	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	430	2	next	next	RB
cord-009318-zt1o1bcz	430	3	assessed	assess	VBD
cord-009318-zt1o1bcz	430	4	whether	whether	IN
cord-009318-zt1o1bcz	430	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	430	6	could	could	MD
cord-009318-zt1o1bcz	430	7	use	use	VB
cord-009318-zt1o1bcz	430	8	HRM	HRM	NNP
cord-009318-zt1o1bcz	430	9	to	to	TO
cord-009318-zt1o1bcz	430	10	compare	compare	VB
cord-009318-zt1o1bcz	430	11	the	the	DT
cord-009318-zt1o1bcz	430	12	performance	performance	NN
cord-009318-zt1o1bcz	430	13	of	of	IN
cord-009318-zt1o1bcz	430	14	the	the	DT
cord-009318-zt1o1bcz	430	15	two	two	CD
cord-009318-zt1o1bcz	430	16	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	430	17	,	,	,
cord-009318-zt1o1bcz	430	18	to	to	TO
cord-009318-zt1o1bcz	430	19	see	see	VB
cord-009318-zt1o1bcz	430	20	which	which	WDT
cord-009318-zt1o1bcz	430	21	one	one	PRP
cord-009318-zt1o1bcz	430	22	would	would	MD
cord-009318-zt1o1bcz	430	23	give	give	VB
cord-009318-zt1o1bcz	430	24	the	the	DT
cord-009318-zt1o1bcz	430	25	best	good	JJS
cord-009318-zt1o1bcz	430	26	LOD	lod	NN
cord-009318-zt1o1bcz	430	27	and	and	CC
cord-009318-zt1o1bcz	430	28	which	which	WDT
cord-009318-zt1o1bcz	430	29	combination	combination	NN
cord-009318-zt1o1bcz	430	30	of	of	IN
cord-009318-zt1o1bcz	430	31	hardware	hardware	NN
cord-009318-zt1o1bcz	430	32	components	component	NNS
cord-009318-zt1o1bcz	430	33	would	would	MD
cord-009318-zt1o1bcz	430	34	give	give	VB
cord-009318-zt1o1bcz	430	35	the	the	DT
cord-009318-zt1o1bcz	430	36	optimum	optimum	JJ
cord-009318-zt1o1bcz	430	37	assay	assay	NN
cord-009318-zt1o1bcz	430	38	performance	performance	NN
cord-009318-zt1o1bcz	430	39	.	.	.
cord-009318-zt1o1bcz	431	1	(	(	-LRB-
cord-009318-zt1o1bcz	431	2	Figure	figure	NN
cord-009318-zt1o1bcz	431	3	7	7	CD
cord-009318-zt1o1bcz	431	4	G	g	NN
cord-009318-zt1o1bcz	431	5	)	)	-RRB-
cord-009318-zt1o1bcz	432	1	For	for	IN
cord-009318-zt1o1bcz	432	2	both	both	DT
cord-009318-zt1o1bcz	432	3	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	432	4	we	-PRON-	PRP
cord-009318-zt1o1bcz	432	5	observed	observe	VBD
cord-009318-zt1o1bcz	432	6	a	a	DT
cord-009318-zt1o1bcz	432	7	similar	similar	JJ
cord-009318-zt1o1bcz	432	8	,	,	,
cord-009318-zt1o1bcz	432	9	rapid	rapid	JJ
cord-009318-zt1o1bcz	432	10	decrease	decrease	NN
cord-009318-zt1o1bcz	432	11	in	in	IN
cord-009318-zt1o1bcz	432	12	LOD	lod	NN
cord-009318-zt1o1bcz	432	13	in	in	IN
cord-009318-zt1o1bcz	432	14	the	the	DT
cord-009318-zt1o1bcz	432	15	initial	initial	JJ
cord-009318-zt1o1bcz	432	16	moments	moment	NNS
cord-009318-zt1o1bcz	432	17	as	as	IN
cord-009318-zt1o1bcz	432	18	true	true	JJ
cord-009318-zt1o1bcz	432	19	-	-	HYPH
cord-009318-zt1o1bcz	432	20	positive	positive	JJ
cord-009318-zt1o1bcz	432	21	events	event	NNS
cord-009318-zt1o1bcz	432	22	are	be	VBP
cord-009318-zt1o1bcz	432	23	detected	detect	VBN
cord-009318-zt1o1bcz	432	24	.	.	.
cord-009318-zt1o1bcz	433	1	However	however	RB
cord-009318-zt1o1bcz	433	2	,	,	,
cord-009318-zt1o1bcz	433	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	433	4	also	also	RB
cord-009318-zt1o1bcz	433	5	noticed	notice	VBD
cord-009318-zt1o1bcz	433	6	several	several	JJ
cord-009318-zt1o1bcz	433	7	differences	difference	NNS
cord-009318-zt1o1bcz	433	8	.	.	.
cord-009318-zt1o1bcz	434	1	Bst	Bst	NNP
cord-009318-zt1o1bcz	434	2	2.0	2.0	CD
cord-009318-zt1o1bcz	434	3	has	have	VBZ
cord-009318-zt1o1bcz	434	4	a	a	DT
cord-009318-zt1o1bcz	434	5	lower	low	JJR
cord-009318-zt1o1bcz	434	6	LOD	lod	NN
cord-009318-zt1o1bcz	434	7	than	than	IN
cord-009318-zt1o1bcz	434	8	Bst	Bst	NNP
cord-009318-zt1o1bcz	434	9	3.0	3.0	NNP
cord-009318-zt1o1bcz	434	10	at	at	IN
cord-009318-zt1o1bcz	434	11	any	any	DT
cord-009318-zt1o1bcz	434	12	amplification	amplification	NN
cord-009318-zt1o1bcz	434	13	time	time	NN
cord-009318-zt1o1bcz	434	14	.	.	.
cord-009318-zt1o1bcz	435	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	435	2	attribute	attribute	VBP
cord-009318-zt1o1bcz	435	3	this	this	DT
cord-009318-zt1o1bcz	435	4	difference	difference	NN
cord-009318-zt1o1bcz	435	5	to	to	IN
cord-009318-zt1o1bcz	435	6	the	the	DT
cord-009318-zt1o1bcz	435	7	higher	high	JJR
cord-009318-zt1o1bcz	435	8	incidence	incidence	NN
cord-009318-zt1o1bcz	435	9	of	of	IN
cord-009318-zt1o1bcz	435	10	false	false	JJ
cord-009318-zt1o1bcz	435	11	positives	positive	NNS
cord-009318-zt1o1bcz	435	12	when	when	WRB
cord-009318-zt1o1bcz	435	13	using	use	VBG
cord-009318-zt1o1bcz	435	14	Bst	Bst	NNP
cord-009318-zt1o1bcz	435	15	3.0	3.0	CD
cord-009318-zt1o1bcz	435	16	compared	compare	VBN
cord-009318-zt1o1bcz	435	17	with	with	IN
cord-009318-zt1o1bcz	435	18	Bst	Bst	NNP
cord-009318-zt1o1bcz	435	19	2.0	2.0	NNP
cord-009318-zt1o1bcz	435	20	.	.	.
cord-009318-zt1o1bcz	436	1	An	an	DT
cord-009318-zt1o1bcz	436	2	additional	additional	JJ
cord-009318-zt1o1bcz	436	3	consequence	consequence	NN
cord-009318-zt1o1bcz	436	4	of	of	IN
cord-009318-zt1o1bcz	436	5	the	the	DT
cord-009318-zt1o1bcz	436	6	low	low	JJ
cord-009318-zt1o1bcz	436	7	false	false	JJ
cord-009318-zt1o1bcz	436	8	-	-	HYPH
cord-009318-zt1o1bcz	436	9	positive	positive	JJ
cord-009318-zt1o1bcz	436	10	incidence	incidence	NN
cord-009318-zt1o1bcz	436	11	using	use	VBG
cord-009318-zt1o1bcz	436	12	Bst	Bst	NNP
cord-009318-zt1o1bcz	436	13	2.0	2.0	CD
cord-009318-zt1o1bcz	436	14	,	,	,
cord-009318-zt1o1bcz	436	15	regardless	regardless	RB
cord-009318-zt1o1bcz	436	16	of	of	IN
cord-009318-zt1o1bcz	436	17	the	the	DT
cord-009318-zt1o1bcz	436	18	use	use	NN
cord-009318-zt1o1bcz	436	19	of	of	IN
cord-009318-zt1o1bcz	436	20	HRM	HRM	NNP
cord-009318-zt1o1bcz	436	21	in	in	IN
cord-009318-zt1o1bcz	436	22	the	the	DT
cord-009318-zt1o1bcz	436	23	assay	assay	NN
cord-009318-zt1o1bcz	436	24	,	,	,
cord-009318-zt1o1bcz	436	25	is	be	VBZ
cord-009318-zt1o1bcz	436	26	the	the	DT
cord-009318-zt1o1bcz	436	27	LOD	LOD	NNP
cord-009318-zt1o1bcz	436	28	continues	continue	VBZ
cord-009318-zt1o1bcz	436	29	to	to	TO
cord-009318-zt1o1bcz	436	30	improve	improve	VB
cord-009318-zt1o1bcz	436	31	with	with	IN
cord-009318-zt1o1bcz	436	32	time	time	NN
cord-009318-zt1o1bcz	436	33	as	as	IN
cord-009318-zt1o1bcz	436	34	additional	additional	JJ
cord-009318-zt1o1bcz	436	35	true	true	JJ
cord-009318-zt1o1bcz	436	36	positives	positive	NNS
cord-009318-zt1o1bcz	436	37	are	be	VBP
cord-009318-zt1o1bcz	436	38	counted	count	VBN
cord-009318-zt1o1bcz	436	39	.	.	.
cord-009318-zt1o1bcz	437	1	In	in	IN
cord-009318-zt1o1bcz	437	2	contrast	contrast	NN
cord-009318-zt1o1bcz	437	3	,	,	,
cord-009318-zt1o1bcz	437	4	Bst	Bst	NNP
cord-009318-zt1o1bcz	437	5	3.0	3.0	CD
cord-009318-zt1o1bcz	437	6	benefits	benefit	NNS
cord-009318-zt1o1bcz	437	7	greatly	greatly	RB
cord-009318-zt1o1bcz	437	8	from	from	IN
cord-009318-zt1o1bcz	437	9	use	use	NN
cord-009318-zt1o1bcz	437	10	of	of	IN
cord-009318-zt1o1bcz	437	11	HRM	HRM	NNP
cord-009318-zt1o1bcz	437	12	in	in	IN
cord-009318-zt1o1bcz	437	13	the	the	DT
cord-009318-zt1o1bcz	437	14	final	final	JJ
cord-009318-zt1o1bcz	437	15	assay	assay	NN
cord-009318-zt1o1bcz	437	16	.	.	.
cord-009318-zt1o1bcz	438	1	If	if	IN
cord-009318-zt1o1bcz	438	2	HRM	HRM	NNP
cord-009318-zt1o1bcz	438	3	is	be	VBZ
cord-009318-zt1o1bcz	438	4	not	not	RB
cord-009318-zt1o1bcz	438	5	included	include	VBN
cord-009318-zt1o1bcz	438	6	in	in	IN
cord-009318-zt1o1bcz	438	7	the	the	DT
cord-009318-zt1o1bcz	438	8	assay	assay	NN
cord-009318-zt1o1bcz	438	9	(	(	-LRB-
cord-009318-zt1o1bcz	438	10	Figure	figure	NN
cord-009318-zt1o1bcz	438	11	7	7	CD
cord-009318-zt1o1bcz	438	12	G	g	NN
cord-009318-zt1o1bcz	438	13	,	,	,
cord-009318-zt1o1bcz	438	14	red	red	JJ
cord-009318-zt1o1bcz	438	15	dashed	dash	VBD
cord-009318-zt1o1bcz	438	16	)	)	-RRB-
cord-009318-zt1o1bcz	438	17	,	,	,
cord-009318-zt1o1bcz	438	18	a	a	DT
cord-009318-zt1o1bcz	438	19	clear	clear	JJ
cord-009318-zt1o1bcz	438	20	optimum	optimum	NN
cord-009318-zt1o1bcz	438	21	for	for	IN
cord-009318-zt1o1bcz	438	22	LOD	lod	NN
cord-009318-zt1o1bcz	438	23	occurs	occur	VBZ
cord-009318-zt1o1bcz	438	24	at	at	IN
cord-009318-zt1o1bcz	438	25	34	34	CD
cord-009318-zt1o1bcz	438	26	min	min	NN
cord-009318-zt1o1bcz	438	27	and	and	CC
cord-009318-zt1o1bcz	438	28	0.93±0.16	0.93±0.16	CD
cord-009318-zt1o1bcz	439	1	cp	cp	NNP
cord-009318-zt1o1bcz	439	2	/	/	SYM
cord-009318-zt1o1bcz	439	3	L.	L.	NNP
cord-009318-zt1o1bcz	440	1	However	however	RB
cord-009318-zt1o1bcz	440	2	,	,	,
cord-009318-zt1o1bcz	440	3	if	if	IN
cord-009318-zt1o1bcz	440	4	HRM	HRM	NNP
cord-009318-zt1o1bcz	440	5	is	be	VBZ
cord-009318-zt1o1bcz	440	6	employed	employ	VBN
cord-009318-zt1o1bcz	440	7	in	in	IN
cord-009318-zt1o1bcz	440	8	the	the	DT
cord-009318-zt1o1bcz	440	9	assay	assay	NN
cord-009318-zt1o1bcz	440	10	,	,	,
cord-009318-zt1o1bcz	440	11	the	the	DT
cord-009318-zt1o1bcz	440	12	LOD	lod	NN
cord-009318-zt1o1bcz	440	13	more	more	RBR
cord-009318-zt1o1bcz	440	14	closely	closely	RB
cord-009318-zt1o1bcz	440	15	resembles	resemble	VBZ
cord-009318-zt1o1bcz	440	16	the	the	DT
cord-009318-zt1o1bcz	440	17	LOD	lod	NN
cord-009318-zt1o1bcz	440	18	curve	curve	NN
cord-009318-zt1o1bcz	440	19	for	for	IN
cord-009318-zt1o1bcz	440	20	Bst	Bst	NNP
cord-009318-zt1o1bcz	440	21	2.0	2.0	NNP
cord-009318-zt1o1bcz	440	22	and	and	CC
cord-009318-zt1o1bcz	440	23	improves	improve	VBZ
cord-009318-zt1o1bcz	440	24	with	with	IN
cord-009318-zt1o1bcz	440	25	increased	increase	VBN
cord-009318-zt1o1bcz	440	26	detection	detection	NN
cord-009318-zt1o1bcz	440	27	of	of	IN
cord-009318-zt1o1bcz	440	28	true	true	JJ
cord-009318-zt1o1bcz	440	29	-	-	HYPH
cord-009318-zt1o1bcz	440	30	positive	positive	JJ
cord-009318-zt1o1bcz	440	31	events	event	NNS
cord-009318-zt1o1bcz	440	32	.	.	.
cord-009318-zt1o1bcz	441	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	441	2	made	make	VBD
cord-009318-zt1o1bcz	441	3	several	several	JJ
cord-009318-zt1o1bcz	441	4	overarching	overarching	JJ
cord-009318-zt1o1bcz	441	5	conclusions	conclusion	NNS
cord-009318-zt1o1bcz	441	6	regarding	regard	VBG
cord-009318-zt1o1bcz	441	7	improving	improve	VBG
cord-009318-zt1o1bcz	441	8	the	the	DT
cord-009318-zt1o1bcz	441	9	LOD	lod	NN
cord-009318-zt1o1bcz	441	10	of	of	IN
cord-009318-zt1o1bcz	441	11	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	441	12	using	use	VBG
cord-009318-zt1o1bcz	441	13	a	a	DT
cord-009318-zt1o1bcz	441	14	combination	combination	NN
cord-009318-zt1o1bcz	441	15	of	of	IN
cord-009318-zt1o1bcz	441	16	digital	digital	JJ
cord-009318-zt1o1bcz	441	17	real	real	JJ
cord-009318-zt1o1bcz	441	18	-	-	HYPH
cord-009318-zt1o1bcz	441	19	time	time	NN
cord-009318-zt1o1bcz	441	20	parameters	parameter	NNS
cord-009318-zt1o1bcz	441	21	and	and	CC
cord-009318-zt1o1bcz	441	22	T	t	NN
cord-009318-zt1o1bcz	441	23	m	m	NN
cord-009318-zt1o1bcz	441	24	.	.	.
cord-009318-zt1o1bcz	442	1	First	first	RB
cord-009318-zt1o1bcz	442	2	,	,	,
cord-009318-zt1o1bcz	442	3	filter	filter	NN
cord-009318-zt1o1bcz	442	4	parameters	parameter	NNS
cord-009318-zt1o1bcz	442	5	can	can	MD
cord-009318-zt1o1bcz	442	6	be	be	VB
cord-009318-zt1o1bcz	442	7	used	use	VBN
cord-009318-zt1o1bcz	442	8	singly	singly	RB
cord-009318-zt1o1bcz	442	9	or	or	CC
cord-009318-zt1o1bcz	442	10	in	in	IN
cord-009318-zt1o1bcz	442	11	combination	combination	NN
cord-009318-zt1o1bcz	442	12	to	to	TO
cord-009318-zt1o1bcz	442	13	improve	improve	VB
cord-009318-zt1o1bcz	442	14	the	the	DT
cord-009318-zt1o1bcz	442	15	performance	performance	NN
cord-009318-zt1o1bcz	442	16	(	(	-LRB-
cord-009318-zt1o1bcz	442	17	LOD	LOD	NNP
cord-009318-zt1o1bcz	442	18	)	)	-RRB-
cord-009318-zt1o1bcz	442	19	of	of	IN
cord-009318-zt1o1bcz	442	20	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	442	21	.	.	.
cord-009318-zt1o1bcz	443	1	In	in	IN
cord-009318-zt1o1bcz	443	2	certain	certain	JJ
cord-009318-zt1o1bcz	443	3	assays	assay	NNS
cord-009318-zt1o1bcz	443	4	one	one	CD
cord-009318-zt1o1bcz	443	5	parameter	parameter	NN
cord-009318-zt1o1bcz	443	6	may	may	MD
cord-009318-zt1o1bcz	443	7	perform	perform	VB
cord-009318-zt1o1bcz	443	8	better	well	RBR
cord-009318-zt1o1bcz	443	9	than	than	IN
cord-009318-zt1o1bcz	443	10	another	another	DT
cord-009318-zt1o1bcz	443	11	for	for	IN
cord-009318-zt1o1bcz	443	12	this	this	DT
cord-009318-zt1o1bcz	443	13	selection	selection	NN
cord-009318-zt1o1bcz	443	14	.	.	.
cord-009318-zt1o1bcz	444	1	For	for	IN
cord-009318-zt1o1bcz	444	2	this	this	DT
cord-009318-zt1o1bcz	444	3	primer	primer	NN
cord-009318-zt1o1bcz	444	4	set	set	NN
cord-009318-zt1o1bcz	444	5	,	,	,
cord-009318-zt1o1bcz	444	6	LOD	LOD	NNP
cord-009318-zt1o1bcz	444	7	for	for	IN
cord-009318-zt1o1bcz	444	8	Bst	Bst	NNP
cord-009318-zt1o1bcz	444	9	3.0	3.0	NNP
cord-009318-zt1o1bcz	444	10	was	be	VBD
cord-009318-zt1o1bcz	444	11	lower	low	JJR
cord-009318-zt1o1bcz	444	12	(	(	-LRB-
cord-009318-zt1o1bcz	444	13	better	well	RBR
cord-009318-zt1o1bcz	444	14	)	)	-RRB-
cord-009318-zt1o1bcz	444	15	when	when	WRB
cord-009318-zt1o1bcz	444	16	using	use	VBG
cord-009318-zt1o1bcz	444	17	TTP	TTP	NNP
cord-009318-zt1o1bcz	444	18	(	(	-LRB-
cord-009318-zt1o1bcz	444	19	0.93±0.16	0.93±0.16	NNP
cord-009318-zt1o1bcz	444	20	cp	cp	NNP
cord-009318-zt1o1bcz	444	21	/	/	SYM
cord-009318-zt1o1bcz	444	22	L	L	NNP
cord-009318-zt1o1bcz	444	23	)	)	-RRB-
cord-009318-zt1o1bcz	444	24	than	than	IN
cord-009318-zt1o1bcz	444	25	max	max	NN
cord-009318-zt1o1bcz	444	26	rate	rate	NN
cord-009318-zt1o1bcz	444	27	(	(	-LRB-
cord-009318-zt1o1bcz	444	28	2.11±0.92	2.11±0.92	CD
cord-009318-zt1o1bcz	444	29	cp	cp	NN
cord-009318-zt1o1bcz	444	30	/	/	CC
cord-009318-zt1o1bcz	444	31	L	L	NNP
cord-009318-zt1o1bcz	444	32	)	)	-RRB-
cord-009318-zt1o1bcz	444	33	or	or	CC
cord-009318-zt1o1bcz	444	34	final	final	JJ
cord-009318-zt1o1bcz	444	35	intensity	intensity	NN
cord-009318-zt1o1bcz	444	36	(	(	-LRB-
cord-009318-zt1o1bcz	444	37	2.14±0.89	2.14±0.89	CD
cord-009318-zt1o1bcz	444	38	cp	cp	NNP
cord-009318-zt1o1bcz	444	39	/	/	SYM
cord-009318-zt1o1bcz	444	40	L	L	NNP
cord-009318-zt1o1bcz	444	41	)	)	-RRB-
cord-009318-zt1o1bcz	444	42	.	.	.
cord-009318-zt1o1bcz	445	1	Second	second	JJ
cord-009318-zt1o1bcz	445	2	,	,	,
cord-009318-zt1o1bcz	445	3	incorporation	incorporation	NN
cord-009318-zt1o1bcz	445	4	of	of	IN
cord-009318-zt1o1bcz	445	5	HRM	HRM	NNP
cord-009318-zt1o1bcz	445	6	into	into	IN
cord-009318-zt1o1bcz	445	7	the	the	DT
cord-009318-zt1o1bcz	445	8	final	final	JJ
cord-009318-zt1o1bcz	445	9	assay	assay	NN
cord-009318-zt1o1bcz	445	10	readout	readout	NN
cord-009318-zt1o1bcz	445	11	will	will	MD
cord-009318-zt1o1bcz	445	12	benefit	benefit	VB
cord-009318-zt1o1bcz	445	13	some	some	DT
cord-009318-zt1o1bcz	445	14	assays	assay	NNS
cord-009318-zt1o1bcz	445	15	more	more	RBR
cord-009318-zt1o1bcz	445	16	than	than	IN
cord-009318-zt1o1bcz	445	17	others	other	NNS
cord-009318-zt1o1bcz	445	18	.	.	.
cord-009318-zt1o1bcz	446	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	446	2	observed	observe	VBD
cord-009318-zt1o1bcz	446	3	incorporation	incorporation	NN
cord-009318-zt1o1bcz	446	4	of	of	IN
cord-009318-zt1o1bcz	446	5	HRM	HRM	NNP
cord-009318-zt1o1bcz	446	6	as	as	IN
cord-009318-zt1o1bcz	446	7	a	a	DT
cord-009318-zt1o1bcz	446	8	part	part	NN
cord-009318-zt1o1bcz	446	9	of	of	IN
cord-009318-zt1o1bcz	446	10	the	the	DT
cord-009318-zt1o1bcz	446	11	final	final	JJ
cord-009318-zt1o1bcz	446	12	assay	assay	NN
cord-009318-zt1o1bcz	446	13	improved	improve	VBD
cord-009318-zt1o1bcz	446	14	the	the	DT
cord-009318-zt1o1bcz	446	15	perofmance	perofmance	NN
cord-009318-zt1o1bcz	446	16	of	of	IN
cord-009318-zt1o1bcz	446	17	Bst	Bst	NNP
cord-009318-zt1o1bcz	446	18	3.0	3.0	CD
cord-009318-zt1o1bcz	446	19	greater	great	JJR
cord-009318-zt1o1bcz	446	20	than	than	IN
cord-009318-zt1o1bcz	446	21	the	the	DT
cord-009318-zt1o1bcz	446	22	perofmance	perofmance	NN
cord-009318-zt1o1bcz	446	23	of	of	IN
cord-009318-zt1o1bcz	446	24	Bst	Bst	NNP
cord-009318-zt1o1bcz	446	25	2.0	2.0	CD
cord-009318-zt1o1bcz	446	26	,	,	,
cord-009318-zt1o1bcz	446	27	and	and	CC
cord-009318-zt1o1bcz	446	28	was	be	VBD
cord-009318-zt1o1bcz	446	29	vital	vital	JJ
cord-009318-zt1o1bcz	446	30	for	for	IN
cord-009318-zt1o1bcz	446	31	long	long	JJ
cord-009318-zt1o1bcz	446	32	assay	assay	NN
cord-009318-zt1o1bcz	446	33	times	time	NNS
cord-009318-zt1o1bcz	446	34	.	.	.
cord-009318-zt1o1bcz	446	35	 	 	_SP
cord-009318-zt1o1bcz	447	1	Assays	assay	NNS
cord-009318-zt1o1bcz	447	2	with	with	IN
cord-009318-zt1o1bcz	447	3	high	high	JJ
cord-009318-zt1o1bcz	447	4	clinical	clinical	JJ
cord-009318-zt1o1bcz	447	5	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	447	6	and	and	CC
cord-009318-zt1o1bcz	447	7	specificity	specificity	NN
cord-009318-zt1o1bcz	447	8	are	be	VBP
cord-009318-zt1o1bcz	447	9	critically	critically	RB
cord-009318-zt1o1bcz	447	10	needed	need	VBN
cord-009318-zt1o1bcz	447	11	.	.	.
cord-009318-zt1o1bcz	448	1	Clinical	clinical	JJ
cord-009318-zt1o1bcz	448	2	samples	sample	NNS
cord-009318-zt1o1bcz	448	3	of	of	IN
cord-009318-zt1o1bcz	448	4	CT	CT	NNP
cord-009318-zt1o1bcz	448	5	,	,	,
cord-009318-zt1o1bcz	448	6	originating	originate	VBG
cord-009318-zt1o1bcz	448	7	from	from	IN
cord-009318-zt1o1bcz	448	8	urine	urine	NN
cord-009318-zt1o1bcz	448	9	and	and	CC
cord-009318-zt1o1bcz	448	10	swabs	swab	NNS
cord-009318-zt1o1bcz	448	11	,	,	,
cord-009318-zt1o1bcz	448	12	pose	pose	VBP
cord-009318-zt1o1bcz	448	13	an	an	DT
cord-009318-zt1o1bcz	448	14	intrinsic	intrinsic	JJ
cord-009318-zt1o1bcz	448	15	challenge	challenge	NN
cord-009318-zt1o1bcz	448	16	because	because	IN
cord-009318-zt1o1bcz	448	17	they	-PRON-	PRP
cord-009318-zt1o1bcz	448	18	contain	contain	VBP
cord-009318-zt1o1bcz	448	19	variable	variable	JJ
cord-009318-zt1o1bcz	448	20	levels	level	NNS
cord-009318-zt1o1bcz	448	21	of	of	IN
cord-009318-zt1o1bcz	448	22	host	host	NN
cord-009318-zt1o1bcz	448	23	DNA	dna	NN
cord-009318-zt1o1bcz	448	24	,	,	,
cord-009318-zt1o1bcz	448	25	and	and	CC
cord-009318-zt1o1bcz	448	26	DNA	dna	NN
cord-009318-zt1o1bcz	448	27	from	from	IN
cord-009318-zt1o1bcz	448	28	other	other	JJ
cord-009318-zt1o1bcz	448	29	flora	flora	NNS
cord-009318-zt1o1bcz	448	30	.	.	.
cord-009318-zt1o1bcz	449	1	The	the	DT
cord-009318-zt1o1bcz	449	2	analysis	analysis	NN
cord-009318-zt1o1bcz	449	3	of	of	IN
cord-009318-zt1o1bcz	449	4	these	these	DT
cord-009318-zt1o1bcz	449	5	clinical	clinical	JJ
cord-009318-zt1o1bcz	449	6	samples	sample	NNS
cord-009318-zt1o1bcz	449	7	,	,	,
cord-009318-zt1o1bcz	449	8	needs	need	VBZ
cord-009318-zt1o1bcz	449	9	not	not	RB
cord-009318-zt1o1bcz	449	10	only	only	RB
cord-009318-zt1o1bcz	449	11	to	to	TO
cord-009318-zt1o1bcz	449	12	be	be	VB
cord-009318-zt1o1bcz	449	13	sensitive	sensitive	JJ
cord-009318-zt1o1bcz	449	14	(	(	-LRB-
cord-009318-zt1o1bcz	449	15	good	good	JJ
cord-009318-zt1o1bcz	449	16	LOD	LOD	NNP
cord-009318-zt1o1bcz	449	17	)	)	-RRB-
cord-009318-zt1o1bcz	449	18	,	,	,
cord-009318-zt1o1bcz	449	19	but	but	CC
cord-009318-zt1o1bcz	449	20	also	also	RB
cord-009318-zt1o1bcz	449	21	able	able	JJ
cord-009318-zt1o1bcz	449	22	to	to	TO
cord-009318-zt1o1bcz	449	23	function	function	VB
cord-009318-zt1o1bcz	449	24	in	in	IN
cord-009318-zt1o1bcz	449	25	the	the	DT
cord-009318-zt1o1bcz	449	26	presence	presence	NN
cord-009318-zt1o1bcz	449	27	of	of	IN
cord-009318-zt1o1bcz	449	28	non	non	JJ
cord-009318-zt1o1bcz	449	29	-	-	JJ
cord-009318-zt1o1bcz	449	30	specific	specific	JJ
cord-009318-zt1o1bcz	449	31	,	,	,
cord-009318-zt1o1bcz	449	32	potentially	potentially	RB
cord-009318-zt1o1bcz	449	33	amplifiable	amplifiable	JJ
cord-009318-zt1o1bcz	449	34	genomic	genomic	JJ
cord-009318-zt1o1bcz	449	35	secondary	secondary	JJ
cord-009318-zt1o1bcz	449	36	structures	structure	NNS
cord-009318-zt1o1bcz	449	37	and	and	CC
cord-009318-zt1o1bcz	449	38	other	other	JJ
cord-009318-zt1o1bcz	449	39	possible	possible	JJ
cord-009318-zt1o1bcz	449	40	environmental	environmental	JJ
cord-009318-zt1o1bcz	449	41	contaminants	contaminant	NNS
cord-009318-zt1o1bcz	449	42	,	,	,
cord-009318-zt1o1bcz	449	43	while	while	IN
cord-009318-zt1o1bcz	449	44	remaining	remain	VBG
cord-009318-zt1o1bcz	449	45	consistent	consistent	JJ
cord-009318-zt1o1bcz	449	46	between	between	IN
cord-009318-zt1o1bcz	449	47	samples	sample	NNS
cord-009318-zt1o1bcz	449	48	.	.	.
cord-009318-zt1o1bcz	450	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	450	2	sought	seek	VBD
cord-009318-zt1o1bcz	450	3	to	to	TO
cord-009318-zt1o1bcz	450	4	investigate	investigate	VB
cord-009318-zt1o1bcz	450	5	the	the	DT
cord-009318-zt1o1bcz	450	6	impact	impact	NN
cord-009318-zt1o1bcz	450	7	of	of	IN
cord-009318-zt1o1bcz	450	8	host	host	NN
cord-009318-zt1o1bcz	450	9	human	human	JJ
cord-009318-zt1o1bcz	450	10	genomic	genomic	JJ
cord-009318-zt1o1bcz	450	11	DNA	dna	NN
cord-009318-zt1o1bcz	450	12	(	(	-LRB-
cord-009318-zt1o1bcz	450	13	hgDNA	hgdna	NN
cord-009318-zt1o1bcz	450	14	)	)	-RRB-
cord-009318-zt1o1bcz	450	15	on	on	IN
cord-009318-zt1o1bcz	450	16	non	non	JJ
cord-009318-zt1o1bcz	450	17	-	-	JJ
cord-009318-zt1o1bcz	450	18	specific	specific	JJ
cord-009318-zt1o1bcz	450	19	background	background	NN
cord-009318-zt1o1bcz	450	20	amplification	amplification	NN
cord-009318-zt1o1bcz	450	21	.	.	.
cord-009318-zt1o1bcz	451	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	451	2	hypothesized	hypothesize	VBD
cord-009318-zt1o1bcz	451	3	that	that	IN
cord-009318-zt1o1bcz	451	4	non	non	JJ
cord-009318-zt1o1bcz	451	5	-	-	JJ
cord-009318-zt1o1bcz	451	6	specific	specific	JJ
cord-009318-zt1o1bcz	451	7	structures	structure	NNS
cord-009318-zt1o1bcz	451	8	(	(	-LRB-
cord-009318-zt1o1bcz	451	9	like	like	IN
cord-009318-zt1o1bcz	451	10	hairpins	hairpin	NNS
cord-009318-zt1o1bcz	451	11	and	and	CC
cord-009318-zt1o1bcz	451	12	regulatory	regulatory	JJ
cord-009318-zt1o1bcz	451	13	elements	element	NNS
cord-009318-zt1o1bcz	451	14	)	)	-RRB-
cord-009318-zt1o1bcz	451	15	,	,	,
cord-009318-zt1o1bcz	451	16	may	may	MD
cord-009318-zt1o1bcz	451	17	amplify	amplify	VB
cord-009318-zt1o1bcz	451	18	in	in	IN
cord-009318-zt1o1bcz	451	19	the	the	DT
cord-009318-zt1o1bcz	451	20	presence	presence	NN
cord-009318-zt1o1bcz	451	21	of	of	IN
cord-009318-zt1o1bcz	451	22	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	451	23	and	and	CC
cord-009318-zt1o1bcz	451	24	contribute	contribute	VB
cord-009318-zt1o1bcz	451	25	to	to	IN
cord-009318-zt1o1bcz	451	26	non	non	JJ
cord-009318-zt1o1bcz	451	27	-	-	JJ
cord-009318-zt1o1bcz	451	28	specific	specific	JJ
cord-009318-zt1o1bcz	451	29	background	background	NN
cord-009318-zt1o1bcz	451	30	amplification	amplification	NN
cord-009318-zt1o1bcz	451	31	.	.	.
cord-009318-zt1o1bcz	452	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	452	2	titrated	titrate	VBD
cord-009318-zt1o1bcz	452	3	sheared	shear	VBD
cord-009318-zt1o1bcz	452	4	buffy	buffy	NNP
cord-009318-zt1o1bcz	452	5	coat	coat	NNP
cord-009318-zt1o1bcz	452	6	gDNA	gDNA	NNP
cord-009318-zt1o1bcz	452	7	(	(	-LRB-
cord-009318-zt1o1bcz	452	8	i.e.	i.e.	FW
cord-009318-zt1o1bcz	452	9	leukocytes	leukocyte	NNS
cord-009318-zt1o1bcz	452	10	)	)	-RRB-
cord-009318-zt1o1bcz	452	11	concentrations	concentration	NNS
cord-009318-zt1o1bcz	452	12	from	from	IN
cord-009318-zt1o1bcz	452	13	zero	zero	CD
cord-009318-zt1o1bcz	452	14	to	to	IN
cord-009318-zt1o1bcz	452	15	2.5	2.5	CD
cord-009318-zt1o1bcz	452	16	×	×	NN
cord-009318-zt1o1bcz	452	17	10	10	CD
cord-009318-zt1o1bcz	452	18	3	3	CD
cord-009318-zt1o1bcz	452	19	cells	cell	NNS
cord-009318-zt1o1bcz	452	20	per	per	IN
cord-009318-zt1o1bcz	452	21	l	l	NN
cord-009318-zt1o1bcz	452	22	,	,	,
cord-009318-zt1o1bcz	452	23	a	a	DT
cord-009318-zt1o1bcz	452	24	concentration	concentration	NN
cord-009318-zt1o1bcz	452	25	2.5×	2.5×	CD
cord-009318-zt1o1bcz	452	26	greater	great	JJR
cord-009318-zt1o1bcz	452	27	than	than	IN
cord-009318-zt1o1bcz	452	28	that	that	DT
cord-009318-zt1o1bcz	452	29	expected	expect	VBN
cord-009318-zt1o1bcz	452	30	to	to	TO
cord-009318-zt1o1bcz	452	31	cause	cause	VB
cord-009318-zt1o1bcz	452	32	interference	interference	NN
cord-009318-zt1o1bcz	452	33	(	(	-LRB-
cord-009318-zt1o1bcz	452	34	8)	8)	NN
cord-009318-zt1o1bcz	452	35	and	and	CC
cord-009318-zt1o1bcz	452	36	observed	observe	VBD
cord-009318-zt1o1bcz	452	37	the	the	DT
cord-009318-zt1o1bcz	452	38	impact	impact	NN
cord-009318-zt1o1bcz	452	39	on	on	IN
cord-009318-zt1o1bcz	452	40	specific	specific	JJ
cord-009318-zt1o1bcz	452	41	and	and	CC
cord-009318-zt1o1bcz	452	42	non	non	JJ
cord-009318-zt1o1bcz	452	43	-	-	JJ
cord-009318-zt1o1bcz	452	44	specific	specific	JJ
cord-009318-zt1o1bcz	452	45	amplification	amplification	NN
cord-009318-zt1o1bcz	452	46	of	of	IN
cord-009318-zt1o1bcz	452	47	CT	CT	NNP
cord-009318-zt1o1bcz	452	48	(	(	-LRB-
cord-009318-zt1o1bcz	452	49	Figure	Figure	NNP
cord-009318-zt1o1bcz	452	50	8	8	CD
cord-009318-zt1o1bcz	452	51	)	)	-RRB-
cord-009318-zt1o1bcz	452	52	.	.	.
cord-009318-zt1o1bcz	453	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	453	2	measured	measure	VBD
cord-009318-zt1o1bcz	453	3	the	the	DT
cord-009318-zt1o1bcz	453	4	concentration	concentration	NN
cord-009318-zt1o1bcz	453	5	of	of	IN
cord-009318-zt1o1bcz	453	6	hgDNA	hgdna	NN
cord-009318-zt1o1bcz	453	7	in	in	IN
cord-009318-zt1o1bcz	453	8	Human	Human	NNP
cord-009318-zt1o1bcz	453	9	Haploid	Haploid	NNP
cord-009318-zt1o1bcz	453	10	Genome	Genome	NNP
cord-009318-zt1o1bcz	453	11	Equivalents	Equivalents	NNP
cord-009318-zt1o1bcz	453	12	(	(	-LRB-
cord-009318-zt1o1bcz	453	13	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	453	14	)	)	-RRB-
cord-009318-zt1o1bcz	453	15	or	or	CC
cord-009318-zt1o1bcz	453	16	half	half	PDT
cord-009318-zt1o1bcz	453	17	the	the	DT
cord-009318-zt1o1bcz	453	18	total	total	JJ
cord-009318-zt1o1bcz	453	19	amount	amount	NN
cord-009318-zt1o1bcz	453	20	of	of	IN
cord-009318-zt1o1bcz	453	21	hgDNA	hgdna	NN
cord-009318-zt1o1bcz	453	22	in	in	IN
cord-009318-zt1o1bcz	453	23	a	a	DT
cord-009318-zt1o1bcz	453	24	diploid	diploid	JJ
cord-009318-zt1o1bcz	453	25	cell	cell	NN
cord-009318-zt1o1bcz	453	26	.	.	.
cord-009318-zt1o1bcz	454	1	For	for	IN
cord-009318-zt1o1bcz	454	2	each	each	DT
cord-009318-zt1o1bcz	454	3	concentration	concentration	NN
cord-009318-zt1o1bcz	454	4	of	of	IN
cord-009318-zt1o1bcz	454	5	host	host	NN
cord-009318-zt1o1bcz	454	6	DNA	dna	NN
cord-009318-zt1o1bcz	454	7	and	and	CC
cord-009318-zt1o1bcz	454	8	enzyme	enzyme	NN
cord-009318-zt1o1bcz	454	9	,	,	,
cord-009318-zt1o1bcz	454	10	we	-PRON-	PRP
cord-009318-zt1o1bcz	454	11	ran	run	VBD
cord-009318-zt1o1bcz	454	12	at	at	RB
cord-009318-zt1o1bcz	454	13	least	least	RBS
cord-009318-zt1o1bcz	454	14	three	three	CD
cord-009318-zt1o1bcz	454	15	chips	chip	NNS
cord-009318-zt1o1bcz	454	16	in	in	IN
cord-009318-zt1o1bcz	454	17	the	the	DT
cord-009318-zt1o1bcz	454	18	presence	presence	NN
cord-009318-zt1o1bcz	454	19	of	of	IN
cord-009318-zt1o1bcz	454	20	CT	CT	NNP
cord-009318-zt1o1bcz	454	21	template	template	NN
cord-009318-zt1o1bcz	454	22	and	and	CC
cord-009318-zt1o1bcz	454	23	three	three	CD
cord-009318-zt1o1bcz	454	24	in	in	IN
cord-009318-zt1o1bcz	454	25	the	the	DT
cord-009318-zt1o1bcz	454	26	absence	absence	NN
cord-009318-zt1o1bcz	454	27	of	of	IN
cord-009318-zt1o1bcz	454	28	template	template	NN
cord-009318-zt1o1bcz	454	29	and	and	CC
cord-009318-zt1o1bcz	454	30	across	across	IN
cord-009318-zt1o1bcz	454	31	multiple	multiple	JJ
cord-009318-zt1o1bcz	454	32	days	day	NNS
cord-009318-zt1o1bcz	454	33	and	and	CC
cord-009318-zt1o1bcz	454	34	sample	sample	VB
cord-009318-zt1o1bcz	454	35	lots	lot	NNS
cord-009318-zt1o1bcz	454	36	.	.	.
cord-009318-zt1o1bcz	455	1	In	in	IN
cord-009318-zt1o1bcz	455	2	total	total	NN
cord-009318-zt1o1bcz	455	3	,	,	,
cord-009318-zt1o1bcz	455	4	we	-PRON-	PRP
cord-009318-zt1o1bcz	455	5	observed	observe	VBD
cord-009318-zt1o1bcz	455	6	1	1	CD
cord-009318-zt1o1bcz	455	7	196	196	CD
cord-009318-zt1o1bcz	455	8	038	038	CD
cord-009318-zt1o1bcz	455	9	different	different	JJ
cord-009318-zt1o1bcz	455	10	reaction	reaction	NN
cord-009318-zt1o1bcz	455	11	partitions	partition	NNS
cord-009318-zt1o1bcz	455	12	.	.	.
cord-009318-zt1o1bcz	456	1	At	at	IN
cord-009318-zt1o1bcz	456	2	the	the	DT
cord-009318-zt1o1bcz	456	3	highest	high	JJS
cord-009318-zt1o1bcz	456	4	concentration	concentration	NN
cord-009318-zt1o1bcz	456	5	of	of	IN
cord-009318-zt1o1bcz	456	6	hgDNA	hgdna	NN
cord-009318-zt1o1bcz	456	7	,	,	,
cord-009318-zt1o1bcz	456	8	there	there	EX
cord-009318-zt1o1bcz	456	9	was	be	VBD
cord-009318-zt1o1bcz	456	10	3	3	CD
cord-009318-zt1o1bcz	456	11	030	030	CD
cord-009318-zt1o1bcz	456	12	000	000	CD
cord-009318-zt1o1bcz	456	13	times	time	NNS
cord-009318-zt1o1bcz	456	14	more	more	RBR
cord-009318-zt1o1bcz	456	15	hgDNA	hgdna	JJ
cord-009318-zt1o1bcz	456	16	than	than	IN
cord-009318-zt1o1bcz	456	17	bacterial	bacterial	JJ
cord-009318-zt1o1bcz	456	18	DNA	dna	NN
cord-009318-zt1o1bcz	456	19	by	by	IN
cord-009318-zt1o1bcz	456	20	mass	mass	NN
cord-009318-zt1o1bcz	456	21	.	.	.
cord-009318-zt1o1bcz	457	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	457	2	first	first	RB
cord-009318-zt1o1bcz	457	3	asked	ask	VBD
cord-009318-zt1o1bcz	457	4	how	how	WRB
cord-009318-zt1o1bcz	457	5	background	background	NN
cord-009318-zt1o1bcz	457	6	DNA	dna	NN
cord-009318-zt1o1bcz	457	7	impacted	impact	VBD
cord-009318-zt1o1bcz	457	8	TTP	TTP	NNP
cord-009318-zt1o1bcz	457	9	qualitatively	qualitatively	RB
cord-009318-zt1o1bcz	457	10	.	.	.
cord-009318-zt1o1bcz	458	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	458	2	observed	observe	VBD
cord-009318-zt1o1bcz	458	3	for	for	IN
cord-009318-zt1o1bcz	458	4	both	both	DT
cord-009318-zt1o1bcz	458	5	Bst	Bst	NNP
cord-009318-zt1o1bcz	458	6	2.0	2.0	NNP
cord-009318-zt1o1bcz	458	7	and	and	CC
cord-009318-zt1o1bcz	458	8	Bst	Bst	NNP
cord-009318-zt1o1bcz	458	9	3.0	3.0	CD
cord-009318-zt1o1bcz	458	10	enzymes	enzyme	NNS
cord-009318-zt1o1bcz	458	11	,	,	,
cord-009318-zt1o1bcz	458	12	specific	specific	JJ
cord-009318-zt1o1bcz	458	13	and	and	CC
cord-009318-zt1o1bcz	458	14	non	non	JJ
cord-009318-zt1o1bcz	458	15	-	-	JJ
cord-009318-zt1o1bcz	458	16	specific	specific	JJ
cord-009318-zt1o1bcz	458	17	amplification	amplification	NN
cord-009318-zt1o1bcz	458	18	were	be	VBD
cord-009318-zt1o1bcz	458	19	qualitative	qualitative	JJ
cord-009318-zt1o1bcz	458	20	similar	similar	JJ
cord-009318-zt1o1bcz	458	21	independent	independent	NN
cord-009318-zt1o1bcz	458	22	of	of	IN
cord-009318-zt1o1bcz	458	23	background	background	NN
cord-009318-zt1o1bcz	458	24	DNA	dna	NN
cord-009318-zt1o1bcz	458	25	concentration	concentration	NN
cord-009318-zt1o1bcz	458	26	below	below	IN
cord-009318-zt1o1bcz	458	27	5000	5000	CD
cord-009318-zt1o1bcz	458	28	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	458	29	per	per	IN
cord-009318-zt1o1bcz	458	30	l.	l.	NN
cord-009318-zt1o1bcz	459	1	As	as	IN
cord-009318-zt1o1bcz	459	2	with	with	IN
cord-009318-zt1o1bcz	459	3	previous	previous	JJ
cord-009318-zt1o1bcz	459	4	measurements	measurement	NNS
cord-009318-zt1o1bcz	459	5	,	,	,
cord-009318-zt1o1bcz	459	6	Bst	Bst	NNP
cord-009318-zt1o1bcz	459	7	2.0	2.0	NNP
cord-009318-zt1o1bcz	459	8	rarely	rarely	RB
cord-009318-zt1o1bcz	459	9	produced	produce	VBD
cord-009318-zt1o1bcz	459	10	low	low	JJ
cord-009318-zt1o1bcz	459	11	-	-	HYPH
cord-009318-zt1o1bcz	459	12	T	t	NN
cord-009318-zt1o1bcz	459	13	m	m	NN
cord-009318-zt1o1bcz	459	14	non	non	JJ
cord-009318-zt1o1bcz	459	15	-	-	JJ
cord-009318-zt1o1bcz	459	16	specific	specific	JJ
cord-009318-zt1o1bcz	459	17	events	event	NNS
cord-009318-zt1o1bcz	459	18	;	;	:
cord-009318-zt1o1bcz	459	19	whereas	whereas	IN
cord-009318-zt1o1bcz	459	20	Bst	Bst	NNP
cord-009318-zt1o1bcz	459	21	3.0	3.0	CD
cord-009318-zt1o1bcz	459	22	produced	produce	VBD
cord-009318-zt1o1bcz	459	23	both	both	DT
cord-009318-zt1o1bcz	459	24	high	high	JJ
cord-009318-zt1o1bcz	459	25	-	-	HYPH
cord-009318-zt1o1bcz	459	26	and	and	CC
cord-009318-zt1o1bcz	459	27	low	low	JJ
cord-009318-zt1o1bcz	459	28	-	-	HYPH
cord-009318-zt1o1bcz	459	29	T	t	NN
cord-009318-zt1o1bcz	459	30	m	m	NN
cord-009318-zt1o1bcz	459	31	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	459	32	events	event	NNS
cord-009318-zt1o1bcz	459	33	.	.	.
cord-009318-zt1o1bcz	460	1	Further	further	RB
cord-009318-zt1o1bcz	460	2	,	,	,
cord-009318-zt1o1bcz	460	3	there	there	EX
cord-009318-zt1o1bcz	460	4	were	be	VBD
cord-009318-zt1o1bcz	460	5	more	more	RBR
cord-009318-zt1o1bcz	460	6	non	non	JJ
cord-009318-zt1o1bcz	460	7	-	-	JJ
cord-009318-zt1o1bcz	460	8	specific	specific	JJ
cord-009318-zt1o1bcz	460	9	amplification	amplification	NN
cord-009318-zt1o1bcz	460	10	events	event	NNS
cord-009318-zt1o1bcz	460	11	for	for	IN
cord-009318-zt1o1bcz	460	12	Bst	Bst	NNP
cord-009318-zt1o1bcz	460	13	3.0	3.0	NNP
cord-009318-zt1o1bcz	460	14	than	than	IN
cord-009318-zt1o1bcz	460	15	Bst	Bst	NNP
cord-009318-zt1o1bcz	460	16	2.0	2.0	CD
cord-009318-zt1o1bcz	460	17	at	at	IN
cord-009318-zt1o1bcz	460	18	both	both	CC
cord-009318-zt1o1bcz	460	19	high	high	JJ
cord-009318-zt1o1bcz	460	20	and	and	CC
cord-009318-zt1o1bcz	460	21	low	low	JJ
cord-009318-zt1o1bcz	460	22	T	t	NN
cord-009318-zt1o1bcz	460	23	m	m	NN
cord-009318-zt1o1bcz	460	24	.	.	.
cord-009318-zt1o1bcz	461	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	461	2	next	next	RB
cord-009318-zt1o1bcz	461	3	asked	ask	VBD
cord-009318-zt1o1bcz	461	4	how	how	WRB
cord-009318-zt1o1bcz	461	5	background	background	NN
cord-009318-zt1o1bcz	461	6	hgDNA	hgdna	JJ
cord-009318-zt1o1bcz	461	7	impacts	impact	NNS
cord-009318-zt1o1bcz	461	8	specific	specific	JJ
cord-009318-zt1o1bcz	461	9	and	and	CC
cord-009318-zt1o1bcz	461	10	non	non	JJ
cord-009318-zt1o1bcz	461	11	-	-	JJ
cord-009318-zt1o1bcz	461	12	specific	specific	JJ
cord-009318-zt1o1bcz	461	13	amplification	amplification	NN
cord-009318-zt1o1bcz	461	14	quantitatively	quantitatively	RB
cord-009318-zt1o1bcz	461	15	.	.	.
cord-009318-zt1o1bcz	462	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	462	2	categorized	categorize	VBD
cord-009318-zt1o1bcz	462	3	amplification	amplification	NN
cord-009318-zt1o1bcz	462	4	events	event	NNS
cord-009318-zt1o1bcz	462	5	as	as	IN
cord-009318-zt1o1bcz	462	6	specific	specific	JJ
cord-009318-zt1o1bcz	462	7	and	and	CC
cord-009318-zt1o1bcz	462	8	non	non	JJ
cord-009318-zt1o1bcz	462	9	-	-	JJ
cord-009318-zt1o1bcz	462	10	specific	specific	JJ
cord-009318-zt1o1bcz	462	11	based	base	VBN
cord-009318-zt1o1bcz	462	12	on	on	IN
cord-009318-zt1o1bcz	462	13	T	T	NNP
cord-009318-zt1o1bcz	462	14	m	m	NNP
cord-009318-zt1o1bcz	462	15	as	as	IN
cord-009318-zt1o1bcz	462	16	previously	previously	RB
cord-009318-zt1o1bcz	462	17	.	.	.
cord-009318-zt1o1bcz	463	1	First	first	RB
cord-009318-zt1o1bcz	463	2	,	,	,
cord-009318-zt1o1bcz	463	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	463	4	asked	ask	VBD
cord-009318-zt1o1bcz	463	5	:	:	:
cord-009318-zt1o1bcz	463	6	Is	be	VBZ
cord-009318-zt1o1bcz	463	7	there	there	EX
cord-009318-zt1o1bcz	463	8	a	a	DT
cord-009318-zt1o1bcz	463	9	relationship	relationship	NN
cord-009318-zt1o1bcz	463	10	between	between	IN
cord-009318-zt1o1bcz	463	11	fraction	fraction	NN
cord-009318-zt1o1bcz	463	12	of	of	IN
cord-009318-zt1o1bcz	463	13	template	template	NN
cord-009318-zt1o1bcz	463	14	molecules	molecule	NNS
cord-009318-zt1o1bcz	463	15	amplified	amplify	VBN
cord-009318-zt1o1bcz	463	16	in	in	IN
cord-009318-zt1o1bcz	463	17	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	463	18	and	and	CC
cord-009318-zt1o1bcz	463	19	amplification	amplification	NN
cord-009318-zt1o1bcz	463	20	time	time	NN
cord-009318-zt1o1bcz	463	21	?	?	.
cord-009318-zt1o1bcz	464	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	464	2	then	then	RB
cord-009318-zt1o1bcz	464	3	determined	determine	VBD
cord-009318-zt1o1bcz	464	4	the	the	DT
cord-009318-zt1o1bcz	464	5	total	total	JJ
cord-009318-zt1o1bcz	464	6	number	number	NN
cord-009318-zt1o1bcz	464	7	of	of	IN
cord-009318-zt1o1bcz	464	8	template	template	NN
cord-009318-zt1o1bcz	464	9	copies	copy	NNS
cord-009318-zt1o1bcz	464	10	loaded	load	VBN
cord-009318-zt1o1bcz	464	11	into	into	IN
cord-009318-zt1o1bcz	464	12	a	a	DT
cord-009318-zt1o1bcz	464	13	chip	chip	NN
cord-009318-zt1o1bcz	464	14	relative	relative	JJ
cord-009318-zt1o1bcz	464	15	to	to	IN
cord-009318-zt1o1bcz	464	16	the	the	DT
cord-009318-zt1o1bcz	464	17	copies	copy	NNS
cord-009318-zt1o1bcz	464	18	measured	measure	VBN
cord-009318-zt1o1bcz	464	19	by	by	IN
cord-009318-zt1o1bcz	464	20	ddPCR	ddPCR	NNP
cord-009318-zt1o1bcz	464	21	.	.	.
cord-009318-zt1o1bcz	465	1	If	if	IN
cord-009318-zt1o1bcz	465	2	amplification	amplification	NN
cord-009318-zt1o1bcz	465	3	initiation	initiation	NN
cord-009318-zt1o1bcz	465	4	is	be	VBZ
cord-009318-zt1o1bcz	465	5	stochastic	stochastic	JJ
cord-009318-zt1o1bcz	465	6	,	,	,
cord-009318-zt1o1bcz	465	7	as	as	IN
cord-009318-zt1o1bcz	465	8	observed	observe	VBN
cord-009318-zt1o1bcz	465	9	in	in	IN
cord-009318-zt1o1bcz	465	10	Figures	figure	NNS
cord-009318-zt1o1bcz	465	11	5F	5f	NN
cord-009318-zt1o1bcz	465	12	and	and	CC
cord-009318-zt1o1bcz	465	13	6A	6a	CD
cord-009318-zt1o1bcz	465	14	-B	-b	ADD
cord-009318-zt1o1bcz	465	15	,	,	,
cord-009318-zt1o1bcz	465	16	does	do	VBZ
cord-009318-zt1o1bcz	465	17	longer	long	RBR
cord-009318-zt1o1bcz	465	18	assay	assay	VB
cord-009318-zt1o1bcz	465	19	time	time	NN
cord-009318-zt1o1bcz	465	20	increase	increase	NN
cord-009318-zt1o1bcz	465	21	'	'	''
cord-009318-zt1o1bcz	465	22	efficiency	efficiency	NN
cord-009318-zt1o1bcz	465	23	'	'	''
cord-009318-zt1o1bcz	465	24	and	and	CC
cord-009318-zt1o1bcz	465	25	thereby	thereby	RB
cord-009318-zt1o1bcz	465	26	improve	improve	VB
cord-009318-zt1o1bcz	465	27	LOD	lod	NN
cord-009318-zt1o1bcz	465	28	when	when	WRB
cord-009318-zt1o1bcz	465	29	using	use	VBG
cord-009318-zt1o1bcz	465	30	T	T	NNP
cord-009318-zt1o1bcz	465	31	m	m	NNP
cord-009318-zt1o1bcz	465	32	(	(	-LRB-
cord-009318-zt1o1bcz	465	33	as	as	IN
cord-009318-zt1o1bcz	465	34	seen	see	VBN
cord-009318-zt1o1bcz	465	35	in	in	IN
cord-009318-zt1o1bcz	465	36	Figure	Figure	NNP
cord-009318-zt1o1bcz	465	37	7C	7C	NNP
cord-009318-zt1o1bcz	465	38	and	and	CC
cord-009318-zt1o1bcz	465	39	F	F	NNP
cord-009318-zt1o1bcz	465	40	)	)	-RRB-
cord-009318-zt1o1bcz	465	41	?	?	.
cord-009318-zt1o1bcz	466	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	466	2	observe	observe	VBP
cord-009318-zt1o1bcz	466	3	that	that	IN
cord-009318-zt1o1bcz	466	4	for	for	IN
cord-009318-zt1o1bcz	466	5	Bst	Bst	NNP
cord-009318-zt1o1bcz	466	6	2.0	2.0	CD
cord-009318-zt1o1bcz	466	7	a	a	DT
cord-009318-zt1o1bcz	466	8	large	large	JJ
cord-009318-zt1o1bcz	466	9	number	number	NN
cord-009318-zt1o1bcz	466	10	of	of	IN
cord-009318-zt1o1bcz	466	11	partitions	partition	NNS
cord-009318-zt1o1bcz	466	12	amplify	amplify	VBP
cord-009318-zt1o1bcz	466	13	at	at	IN
cord-009318-zt1o1bcz	466	14	in	in	IN
cord-009318-zt1o1bcz	466	15	the	the	DT
cord-009318-zt1o1bcz	466	16	first	first	JJ
cord-009318-zt1o1bcz	466	17	11.5	11.5	CD
cord-009318-zt1o1bcz	466	18	min	min	NN
cord-009318-zt1o1bcz	466	19	,	,	,
cord-009318-zt1o1bcz	466	20	followed	follow	VBN
cord-009318-zt1o1bcz	466	21	by	by	IN
cord-009318-zt1o1bcz	466	22	a	a	DT
cord-009318-zt1o1bcz	466	23	second	second	JJ
cord-009318-zt1o1bcz	466	24	phase	phase	NN
cord-009318-zt1o1bcz	466	25	after	after	IN
cord-009318-zt1o1bcz	466	26	20	20	CD
cord-009318-zt1o1bcz	466	27	min	min	NN
cord-009318-zt1o1bcz	466	28	where	where	WRB
cord-009318-zt1o1bcz	466	29	additional	additional	JJ
cord-009318-zt1o1bcz	466	30	partitions	partition	NNS
cord-009318-zt1o1bcz	466	31	amplify	amplify	VBP
cord-009318-zt1o1bcz	466	32	with	with	IN
cord-009318-zt1o1bcz	466	33	lower	low	JJR
cord-009318-zt1o1bcz	466	34	frequency	frequency	NN
cord-009318-zt1o1bcz	466	35	(	(	-LRB-
cord-009318-zt1o1bcz	466	36	Figure	figure	NN
cord-009318-zt1o1bcz	466	37	9A	9a	NN
cord-009318-zt1o1bcz	466	38	)	)	-RRB-
cord-009318-zt1o1bcz	466	39	.	.	.
cord-009318-zt1o1bcz	467	1	The	the	DT
cord-009318-zt1o1bcz	467	2	mode	mode	NN
cord-009318-zt1o1bcz	467	3	TTP	ttp	NN
cord-009318-zt1o1bcz	467	4	for	for	IN
cord-009318-zt1o1bcz	467	5	concentrations	concentration	NNS
cord-009318-zt1o1bcz	467	6	less	less	JJR
cord-009318-zt1o1bcz	467	7	than	than	IN
cord-009318-zt1o1bcz	467	8	5000	5000	CD
cord-009318-zt1o1bcz	467	9	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	467	10	per	per	IN
cord-009318-zt1o1bcz	467	11	l	l	NN
cord-009318-zt1o1bcz	467	12	was	be	VBD
cord-009318-zt1o1bcz	467	13	∼11.6	∼11.6	NNP
cord-009318-zt1o1bcz	467	14	±	±	NNP
cord-009318-zt1o1bcz	467	15	0.2	0.2	CD
cord-009318-zt1o1bcz	467	16	min	min	NNP
cord-009318-zt1o1bcz	467	17	(	(	-LRB-
cord-009318-zt1o1bcz	467	18	Supplementary	supplementary	JJ
cord-009318-zt1o1bcz	467	19	Table	Table	NNP
cord-009318-zt1o1bcz	467	20	S8	S8	NNP
cord-009318-zt1o1bcz	467	21	and	and	CC
cord-009318-zt1o1bcz	467	22	Figures	figure	NNS
cord-009318-zt1o1bcz	467	23	S10A-11C	s10a-11c	CD
cord-009318-zt1o1bcz	467	24	)	)	-RRB-
cord-009318-zt1o1bcz	467	25	.	.	.
cord-009318-zt1o1bcz	468	1	After	after	IN
cord-009318-zt1o1bcz	468	2	the	the	DT
cord-009318-zt1o1bcz	468	3	mode	mode	NN
cord-009318-zt1o1bcz	468	4	TTP	ttp	NN
cord-009318-zt1o1bcz	468	5	,	,	,
cord-009318-zt1o1bcz	468	6	the	the	DT
cord-009318-zt1o1bcz	468	7	frequency	frequency	NN
cord-009318-zt1o1bcz	468	8	of	of	IN
cord-009318-zt1o1bcz	468	9	observing	observe	VBG
cord-009318-zt1o1bcz	468	10	specific	specific	JJ
cord-009318-zt1o1bcz	468	11	amplification	amplification	NN
cord-009318-zt1o1bcz	468	12	in	in	IN
cord-009318-zt1o1bcz	468	13	the	the	DT
cord-009318-zt1o1bcz	468	14	absence	absence	NN
cord-009318-zt1o1bcz	468	15	of	of	IN
cord-009318-zt1o1bcz	468	16	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	468	17	decreases	decrease	VBZ
cord-009318-zt1o1bcz	468	18	from	from	IN
cord-009318-zt1o1bcz	468	19	a	a	DT
cord-009318-zt1o1bcz	468	20	maximum	maximum	JJ
cord-009318-zt1o1bcz	468	21	frequency	frequency	NN
cord-009318-zt1o1bcz	468	22	of	of	IN
cord-009318-zt1o1bcz	468	23	1.2	1.2	CD
cord-009318-zt1o1bcz	468	24	±	±	CD
cord-009318-zt1o1bcz	468	25	0.1	0.1	CD
cord-009318-zt1o1bcz	468	26	%	%	NN
cord-009318-zt1o1bcz	468	27	copies	copy	NNS
cord-009318-zt1o1bcz	468	28	detected	detect	VBN
cord-009318-zt1o1bcz	468	29	per	per	IN
cord-009318-zt1o1bcz	468	30	30	30	CD
cord-009318-zt1o1bcz	468	31	s	s	NN
cord-009318-zt1o1bcz	468	32	to	to	IN
cord-009318-zt1o1bcz	468	33	a	a	DT
cord-009318-zt1o1bcz	468	34	lower	low	JJR
cord-009318-zt1o1bcz	468	35	average	average	NN
cord-009318-zt1o1bcz	468	36	fre-	fre-	JJ
cord-009318-zt1o1bcz	468	37	quency	quency	NN
cord-009318-zt1o1bcz	468	38	of	of	IN
cord-009318-zt1o1bcz	468	39	0.23	0.23	CD
cord-009318-zt1o1bcz	468	40	±	±	CD
cord-009318-zt1o1bcz	468	41	0.04	0.04	CD
cord-009318-zt1o1bcz	468	42	%	%	NN
cord-009318-zt1o1bcz	468	43	copies	copy	NNS
cord-009318-zt1o1bcz	468	44	per	per	IN
cord-009318-zt1o1bcz	468	45	30	30	CD
cord-009318-zt1o1bcz	468	46	s	s	NN
cord-009318-zt1o1bcz	468	47	from	from	IN
cord-009318-zt1o1bcz	468	48	20	20	CD
cord-009318-zt1o1bcz	468	49	to	to	TO
cord-009318-zt1o1bcz	468	50	90	90	CD
cord-009318-zt1o1bcz	468	51	min	min	NN
cord-009318-zt1o1bcz	468	52	(	(	-LRB-
cord-009318-zt1o1bcz	468	53	Figure	figure	NN
cord-009318-zt1o1bcz	468	54	9A	9a	NN
cord-009318-zt1o1bcz	468	55	)	)	-RRB-
cord-009318-zt1o1bcz	468	56	.	.	.
cord-009318-zt1o1bcz	469	1	For	for	IN
cord-009318-zt1o1bcz	469	2	Bst	Bst	NNP
cord-009318-zt1o1bcz	469	3	3.0	3.0	NNP
cord-009318-zt1o1bcz	469	4	(	(	-LRB-
cord-009318-zt1o1bcz	469	5	Figure	figure	NN
cord-009318-zt1o1bcz	469	6	10A	10a	CD
cord-009318-zt1o1bcz	469	7	)	)	-RRB-
cord-009318-zt1o1bcz	469	8	,	,	,
cord-009318-zt1o1bcz	469	9	we	-PRON-	PRP
cord-009318-zt1o1bcz	469	10	observe	observe	VBP
cord-009318-zt1o1bcz	469	11	a	a	DT
cord-009318-zt1o1bcz	469	12	similar	similar	JJ
cord-009318-zt1o1bcz	469	13	trend	trend	NN
cord-009318-zt1o1bcz	469	14	temporally	temporally	RB
cord-009318-zt1o1bcz	469	15	,	,	,
cord-009318-zt1o1bcz	469	16	though	though	IN
cord-009318-zt1o1bcz	469	17	mode	mode	NN
cord-009318-zt1o1bcz	469	18	TTP	TTP	NNP
cord-009318-zt1o1bcz	469	19	was	be	VBD
cord-009318-zt1o1bcz	469	20	at	at	RB
cord-009318-zt1o1bcz	469	21	least	least	JJS
cord-009318-zt1o1bcz	469	22	2	2	CD
cord-009318-zt1o1bcz	469	23	min	min	NN
cord-009318-zt1o1bcz	469	24	slower	slow	RBR
cord-009318-zt1o1bcz	469	25	and	and	CC
cord-009318-zt1o1bcz	469	26	had	have	VBD
cord-009318-zt1o1bcz	469	27	greater	great	JJR
cord-009318-zt1o1bcz	469	28	variability	variability	NN
cord-009318-zt1o1bcz	469	29	than	than	IN
cord-009318-zt1o1bcz	469	30	Bst	Bst	NNP
cord-009318-zt1o1bcz	469	31	2.0	2.0	CD
cord-009318-zt1o1bcz	469	32	(	(	-LRB-
cord-009318-zt1o1bcz	469	33	Supplementary	supplementary	JJ
cord-009318-zt1o1bcz	469	34	Table	table	NN
cord-009318-zt1o1bcz	469	35	S8	S8	NNP
cord-009318-zt1o1bcz	469	36	,	,	,
cord-009318-zt1o1bcz	469	37	Figures	figure	NNS
cord-009318-zt1o1bcz	469	38	S10B-11D	s10b-11d	CD
cord-009318-zt1o1bcz	469	39	)	)	-RRB-
cord-009318-zt1o1bcz	469	40	.	.	.
cord-009318-zt1o1bcz	470	1	Further	further	RB
cord-009318-zt1o1bcz	470	2	,	,	,
cord-009318-zt1o1bcz	470	3	Bst	Bst	NNP
cord-009318-zt1o1bcz	470	4	3.0	3.0	NNP
cord-009318-zt1o1bcz	470	5	consistently	consistently	RB
cord-009318-zt1o1bcz	470	6	amplified	amplify	VBD
cord-009318-zt1o1bcz	470	7	fewer	few	JJR
cord-009318-zt1o1bcz	470	8	target	target	NN
cord-009318-zt1o1bcz	470	9	molecules	molecule	NNS
cord-009318-zt1o1bcz	470	10	than	than	IN
cord-009318-zt1o1bcz	470	11	Bst	Bst	NNP
cord-009318-zt1o1bcz	470	12	2.0	2.0	CD
cord-009318-zt1o1bcz	470	13	at	at	IN
cord-009318-zt1o1bcz	470	14	all	all	DT
cord-009318-zt1o1bcz	470	15	time	time	NN
cord-009318-zt1o1bcz	470	16	points	point	NNS
cord-009318-zt1o1bcz	470	17	.	.	.
cord-009318-zt1o1bcz	471	1	This	this	DT
cord-009318-zt1o1bcz	471	2	highlights	highlight	VBZ
cord-009318-zt1o1bcz	471	3	the	the	DT
cord-009318-zt1o1bcz	471	4	stochastic	stochastic	JJ
cord-009318-zt1o1bcz	471	5	nature	nature	NN
cord-009318-zt1o1bcz	471	6	of	of	IN
cord-009318-zt1o1bcz	471	7	amplification	amplification	NN
cord-009318-zt1o1bcz	471	8	using	use	VBG
cord-009318-zt1o1bcz	471	9	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	471	10	,	,	,
cord-009318-zt1o1bcz	471	11	and	and	CC
cord-009318-zt1o1bcz	471	12	importance	importance	NN
cord-009318-zt1o1bcz	471	13	in	in	IN
cord-009318-zt1o1bcz	471	14	choice	choice	NN
cord-009318-zt1o1bcz	471	15	of	of	IN
cord-009318-zt1o1bcz	471	16	enzyme	enzyme	NN
cord-009318-zt1o1bcz	471	17	on	on	IN
cord-009318-zt1o1bcz	471	18	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	471	19	.	.	.
cord-009318-zt1o1bcz	472	1	In	in	IN
cord-009318-zt1o1bcz	472	2	theory	theory	NN
cord-009318-zt1o1bcz	472	3	,	,	,
cord-009318-zt1o1bcz	472	4	assays	assay	NNS
cord-009318-zt1o1bcz	472	5	employing	employ	VBG
cord-009318-zt1o1bcz	472	6	T	t	NN
cord-009318-zt1o1bcz	472	7	m	m	NN
cord-009318-zt1o1bcz	472	8	could	could	MD
cord-009318-zt1o1bcz	472	9	be	be	VB
cord-009318-zt1o1bcz	472	10	run	run	VBN
cord-009318-zt1o1bcz	472	11	until	until	IN
cord-009318-zt1o1bcz	472	12	all	all	DT
cord-009318-zt1o1bcz	472	13	partitions	partition	NNS
cord-009318-zt1o1bcz	472	14	amplify	amplify	VBP
cord-009318-zt1o1bcz	472	15	as	as	IN
cord-009318-zt1o1bcz	472	16	either	either	CC
cord-009318-zt1o1bcz	472	17	a	a	DT
cord-009318-zt1o1bcz	472	18	false	false	JJ
cord-009318-zt1o1bcz	472	19	or	or	CC
cord-009318-zt1o1bcz	472	20	true	true	JJ
cord-009318-zt1o1bcz	472	21	positive	positive	JJ
cord-009318-zt1o1bcz	472	22	.	.	.
cord-009318-zt1o1bcz	473	1	Allow	allow	VB
cord-009318-zt1o1bcz	473	2	all	all	DT
cord-009318-zt1o1bcz	473	3	partitions	partition	NNS
cord-009318-zt1o1bcz	473	4	to	to	TO
cord-009318-zt1o1bcz	473	5	amplify	amplify	VB
cord-009318-zt1o1bcz	473	6	would	would	MD
cord-009318-zt1o1bcz	473	7	give	give	VB
cord-009318-zt1o1bcz	473	8	the	the	DT
cord-009318-zt1o1bcz	473	9	highest	high	JJS
cord-009318-zt1o1bcz	473	10	possible	possible	JJ
cord-009318-zt1o1bcz	473	11	number	number	NN
cord-009318-zt1o1bcz	473	12	of	of	IN
cord-009318-zt1o1bcz	473	13	target	target	NN
cord-009318-zt1o1bcz	473	14	copies	copy	NNS
cord-009318-zt1o1bcz	473	15	amplified	amplify	VBN
cord-009318-zt1o1bcz	473	16	and	and	CC
cord-009318-zt1o1bcz	473	17	lowest	low	JJS
cord-009318-zt1o1bcz	473	18	possible	possible	JJ
cord-009318-zt1o1bcz	473	19	LOD	lod	NN
cord-009318-zt1o1bcz	473	20	when	when	WRB
cord-009318-zt1o1bcz	473	21	using	use	VBG
cord-009318-zt1o1bcz	473	22	T	T	NNP
cord-009318-zt1o1bcz	473	23	m	m	NNP
cord-009318-zt1o1bcz	473	24	in	in	IN
cord-009318-zt1o1bcz	473	25	the	the	DT
cord-009318-zt1o1bcz	473	26	final	final	JJ
cord-009318-zt1o1bcz	473	27	assay	assay	NN
cord-009318-zt1o1bcz	473	28	.	.	.
cord-009318-zt1o1bcz	474	1	Second	second	RB
cord-009318-zt1o1bcz	474	2	,	,	,
cord-009318-zt1o1bcz	474	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	474	4	asked	ask	VBD
cord-009318-zt1o1bcz	474	5	what	what	WP
cord-009318-zt1o1bcz	474	6	is	be	VBZ
cord-009318-zt1o1bcz	474	7	the	the	DT
cord-009318-zt1o1bcz	474	8	impact	impact	NN
cord-009318-zt1o1bcz	474	9	of	of	IN
cord-009318-zt1o1bcz	474	10	hgDNA	hgdna	NN
cord-009318-zt1o1bcz	474	11	on	on	IN
cord-009318-zt1o1bcz	474	12	efficiency	efficiency	NN
cord-009318-zt1o1bcz	474	13	as	as	IN
cord-009318-zt1o1bcz	474	14	a	a	DT
cord-009318-zt1o1bcz	474	15	function	function	NN
cord-009318-zt1o1bcz	474	16	of	of	IN
cord-009318-zt1o1bcz	474	17	time	time	NN
cord-009318-zt1o1bcz	474	18	?	?	.
cord-009318-zt1o1bcz	475	1	For	for	IN
cord-009318-zt1o1bcz	475	2	both	both	DT
cord-009318-zt1o1bcz	475	3	Bst	Bst	NNP
cord-009318-zt1o1bcz	475	4	2.0	2.0	NNP
cord-009318-zt1o1bcz	475	5	and	and	CC
cord-009318-zt1o1bcz	475	6	3.0	3.0	CD
cord-009318-zt1o1bcz	475	7	(	(	-LRB-
cord-009318-zt1o1bcz	475	8	Figures	figure	NNS
cord-009318-zt1o1bcz	475	9	9A	9a	NN
cord-009318-zt1o1bcz	475	10	and	and	CC
cord-009318-zt1o1bcz	475	11	10A	10a	NN
cord-009318-zt1o1bcz	475	12	)	)	-RRB-
cord-009318-zt1o1bcz	475	13	,	,	,
cord-009318-zt1o1bcz	475	14	when	when	WRB
cord-009318-zt1o1bcz	475	15	comparing	compare	VBG
cord-009318-zt1o1bcz	475	16	within	within	IN
cord-009318-zt1o1bcz	475	17	a	a	DT
cord-009318-zt1o1bcz	475	18	given	give	VBN
cord-009318-zt1o1bcz	475	19	enzyme	enzyme	NN
cord-009318-zt1o1bcz	475	20	,	,	,
cord-009318-zt1o1bcz	475	21	we	-PRON-	PRP
cord-009318-zt1o1bcz	475	22	observed	observe	VBD
cord-009318-zt1o1bcz	475	23	that	that	IN
cord-009318-zt1o1bcz	475	24	the	the	DT
cord-009318-zt1o1bcz	475	25	fraction	fraction	NN
cord-009318-zt1o1bcz	475	26	of	of	IN
cord-009318-zt1o1bcz	475	27	copies	copy	NNS
cord-009318-zt1o1bcz	475	28	detected	detect	VBN
cord-009318-zt1o1bcz	475	29	and	and	CC
cord-009318-zt1o1bcz	475	30	the	the	DT
cord-009318-zt1o1bcz	475	31	moment	moment	NN
cord-009318-zt1o1bcz	475	32	the	the	DT
cord-009318-zt1o1bcz	475	33	majority	majority	NN
cord-009318-zt1o1bcz	475	34	of	of	IN
cord-009318-zt1o1bcz	475	35	reactions	reaction	NNS
cord-009318-zt1o1bcz	475	36	initiate	initiate	VBP
cord-009318-zt1o1bcz	475	37	,	,	,
cord-009318-zt1o1bcz	475	38	were	be	VBD
cord-009318-zt1o1bcz	475	39	indistinguishable	indistinguishable	JJ
cord-009318-zt1o1bcz	475	40	for	for	IN
cord-009318-zt1o1bcz	475	41	concentrations	concentration	NNS
cord-009318-zt1o1bcz	475	42	less	less	JJR
cord-009318-zt1o1bcz	475	43	than	than	IN
cord-009318-zt1o1bcz	475	44	5000	5000	CD
cord-009318-zt1o1bcz	475	45	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	475	46	per	per	IN
cord-009318-zt1o1bcz	475	47	l.	l.	NN
cord-009318-zt1o1bcz	476	1	At	at	IN
cord-009318-zt1o1bcz	476	2	5000	5000	CD
cord-009318-zt1o1bcz	476	3	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	476	4	per	per	IN
cord-009318-zt1o1bcz	476	5	l	l	NN
cord-009318-zt1o1bcz	476	6	,	,	,
cord-009318-zt1o1bcz	476	7	a	a	DT
cord-009318-zt1o1bcz	476	8	decrease	decrease	NN
cord-009318-zt1o1bcz	476	9	in	in	IN
cord-009318-zt1o1bcz	476	10	the	the	DT
cord-009318-zt1o1bcz	476	11	fraction	fraction	NN
cord-009318-zt1o1bcz	476	12	of	of	IN
cord-009318-zt1o1bcz	476	13	copies	copy	NNS
cord-009318-zt1o1bcz	476	14	detected	detect	VBN
cord-009318-zt1o1bcz	476	15	and	and	CC
cord-009318-zt1o1bcz	476	16	a	a	DT
cord-009318-zt1o1bcz	476	17	delay	delay	NN
cord-009318-zt1o1bcz	476	18	in	in	IN
cord-009318-zt1o1bcz	476	19	amplification	amplification	NN
cord-009318-zt1o1bcz	476	20	initiation	initiation	NN
cord-009318-zt1o1bcz	476	21	was	be	VBD
cord-009318-zt1o1bcz	476	22	observed	observe	VBN
cord-009318-zt1o1bcz	476	23	(	(	-LRB-
cord-009318-zt1o1bcz	476	24	see	see	VB
cord-009318-zt1o1bcz	476	25	also	also	RB
cord-009318-zt1o1bcz	476	26	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	476	27	Figure	Figure	NNP
cord-009318-zt1o1bcz	476	28	S11C	S11C	NNP
cord-009318-zt1o1bcz	476	29	and	and	CC
cord-009318-zt1o1bcz	476	30	D	D	NNP
cord-009318-zt1o1bcz	476	31	)	)	-RRB-
cord-009318-zt1o1bcz	476	32	.	.	.
cord-009318-zt1o1bcz	477	1	Bst	Bst	NNP
cord-009318-zt1o1bcz	477	2	2.0	2.0	CD
cord-009318-zt1o1bcz	477	3	had	have	VBD
cord-009318-zt1o1bcz	477	4	a	a	DT
cord-009318-zt1o1bcz	477	5	mode	mode	NN
cord-009318-zt1o1bcz	477	6	TTP	ttp	NN
cord-009318-zt1o1bcz	477	7	of	of	IN
cord-009318-zt1o1bcz	477	8	delay	delay	NN
cord-009318-zt1o1bcz	477	9	of	of	IN
cord-009318-zt1o1bcz	477	10	4.7	4.7	CD
cord-009318-zt1o1bcz	477	11	min	min	NN
cord-009318-zt1o1bcz	477	12	to	to	IN
cord-009318-zt1o1bcz	477	13	16.3	16.3	CD
cord-009318-zt1o1bcz	478	1	±	±	NNP
cord-009318-zt1o1bcz	478	2	2.7	2.7	CD
cord-009318-zt1o1bcz	478	3	min	min	NN
cord-009318-zt1o1bcz	478	4	,	,	,
cord-009318-zt1o1bcz	478	5	whereas	whereas	IN
cord-009318-zt1o1bcz	478	6	in	in	IN
cord-009318-zt1o1bcz	478	7	Bst	Bst	NNP
cord-009318-zt1o1bcz	478	8	3.0	3.0	NNP
cord-009318-zt1o1bcz	478	9	,	,	,
cord-009318-zt1o1bcz	478	10	the	the	DT
cord-009318-zt1o1bcz	478	11	mode	mode	NN
cord-009318-zt1o1bcz	478	12	TTP	TTP	NNP
cord-009318-zt1o1bcz	478	13	was	be	VBD
cord-009318-zt1o1bcz	478	14	17.2	17.2	CD
cord-009318-zt1o1bcz	478	15	±	±	NNP
cord-009318-zt1o1bcz	478	16	2.1	2.1	CD
cord-009318-zt1o1bcz	478	17	min	min	NN
cord-009318-zt1o1bcz	478	18	at	at	IN
cord-009318-zt1o1bcz	478	19	5000	5000	CD
cord-009318-zt1o1bcz	478	20	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	478	21	per	per	IN
cord-009318-zt1o1bcz	478	22	l	l	FW
cord-009318-zt1o1bcz	478	23	(	(	-LRB-
cord-009318-zt1o1bcz	478	24	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	478	25	Table	Table	NNP
cord-009318-zt1o1bcz	478	26	S8	S8	NNP
cord-009318-zt1o1bcz	478	27	and	and	CC
cord-009318-zt1o1bcz	478	28	Figure	Figure	NNP
cord-009318-zt1o1bcz	478	29	S11	S11	NNP
cord-009318-zt1o1bcz	478	30	)	)	-RRB-
cord-009318-zt1o1bcz	478	31	.	.	.
cord-009318-zt1o1bcz	479	1	Thus	thus	RB
cord-009318-zt1o1bcz	479	2	,	,	,
cord-009318-zt1o1bcz	479	3	high	high	JJ
cord-009318-zt1o1bcz	479	4	concentrations	concentration	NNS
cord-009318-zt1o1bcz	479	5	of	of	IN
cord-009318-zt1o1bcz	479	6	hgDNA	hgDNA	VBG
cord-009318-zt1o1bcz	479	7	may	may	MD
cord-009318-zt1o1bcz	479	8	suppress	suppress	VB
cord-009318-zt1o1bcz	479	9	specific	specific	JJ
cord-009318-zt1o1bcz	479	10	amplification	amplification	NN
cord-009318-zt1o1bcz	479	11	.	.	.
cord-009318-zt1o1bcz	480	1	Third	third	RB
cord-009318-zt1o1bcz	480	2	,	,	,
cord-009318-zt1o1bcz	480	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	480	4	asked	ask	VBD
cord-009318-zt1o1bcz	480	5	what	what	WP
cord-009318-zt1o1bcz	480	6	is	be	VBZ
cord-009318-zt1o1bcz	480	7	the	the	DT
cord-009318-zt1o1bcz	480	8	impact	impact	NN
cord-009318-zt1o1bcz	480	9	of	of	IN
cord-009318-zt1o1bcz	480	10	hgDNA	hgdna	NN
cord-009318-zt1o1bcz	480	11	and	and	CC
cord-009318-zt1o1bcz	480	12	time	time	NN
cord-009318-zt1o1bcz	480	13	on	on	IN
cord-009318-zt1o1bcz	480	14	non	non	JJ
cord-009318-zt1o1bcz	480	15	-	-	JJ
cord-009318-zt1o1bcz	480	16	specific	specific	JJ
cord-009318-zt1o1bcz	480	17	amplification	amplification	NN
cord-009318-zt1o1bcz	480	18	?	?	.
cord-009318-zt1o1bcz	481	1	For	for	IN
cord-009318-zt1o1bcz	481	2	Bst	Bst	NNP
cord-009318-zt1o1bcz	481	3	2.0	2.0	NNP
cord-009318-zt1o1bcz	481	4	,	,	,
cord-009318-zt1o1bcz	481	5	we	-PRON-	PRP
cord-009318-zt1o1bcz	481	6	observed	observe	VBD
cord-009318-zt1o1bcz	481	7	consistent	consistent	JJ
cord-009318-zt1o1bcz	481	8	non	non	JJ
cord-009318-zt1o1bcz	481	9	-	-	JJ
cord-009318-zt1o1bcz	481	10	specific	specific	JJ
cord-009318-zt1o1bcz	481	11	amplification	amplification	NN
cord-009318-zt1o1bcz	481	12	products	product	NNS
cord-009318-zt1o1bcz	481	13	with	with	IN
cord-009318-zt1o1bcz	481	14	high	high	JJ
cord-009318-zt1o1bcz	481	15	and	and	CC
cord-009318-zt1o1bcz	481	16	low	low	JJ
cord-009318-zt1o1bcz	481	17	T	t	NN
cord-009318-zt1o1bcz	481	18	m	m	NN
cord-009318-zt1o1bcz	481	19	,	,	,
cord-009318-zt1o1bcz	481	20	regardless	regardless	RB
cord-009318-zt1o1bcz	481	21	of	of	IN
cord-009318-zt1o1bcz	481	22	concentration	concentration	NN
cord-009318-zt1o1bcz	481	23	of	of	IN
cord-009318-zt1o1bcz	481	24	hgDNA	hgdna	NN
cord-009318-zt1o1bcz	481	25	.	.	.
cord-009318-zt1o1bcz	482	1	Single	single	JJ
cord-009318-zt1o1bcz	482	2	digital	digital	JJ
cord-009318-zt1o1bcz	482	3	partition	partition	NN
cord-009318-zt1o1bcz	482	4	counts	count	NNS
cord-009318-zt1o1bcz	482	5	were	be	VBD
cord-009318-zt1o1bcz	482	6	observed	observe	VBN
cord-009318-zt1o1bcz	482	7	at	at	IN
cord-009318-zt1o1bcz	482	8	low	low	JJ
cord-009318-zt1o1bcz	482	9	-	-	HYPH
cord-009318-zt1o1bcz	482	10	T	t	NN
cord-009318-zt1o1bcz	482	11	m	m	NN
cord-009318-zt1o1bcz	482	12	non	non	JJ
cord-009318-zt1o1bcz	482	13	-	-	JJ
cord-009318-zt1o1bcz	482	14	specific	specific	JJ
cord-009318-zt1o1bcz	482	15	amplification	amplification	NN
cord-009318-zt1o1bcz	482	16	in	in	IN
cord-009318-zt1o1bcz	482	17	both	both	CC
cord-009318-zt1o1bcz	482	18	the	the	DT
cord-009318-zt1o1bcz	482	19	presence	presence	NN
cord-009318-zt1o1bcz	482	20	of	of	IN
cord-009318-zt1o1bcz	482	21	template	template	NN
cord-009318-zt1o1bcz	482	22	and	and	CC
cord-009318-zt1o1bcz	482	23	the	the	DT
cord-009318-zt1o1bcz	482	24	NTC	NTC	NNP
cord-009318-zt1o1bcz	482	25	and	and	CC
cord-009318-zt1o1bcz	482	26	independent	independent	JJ
cord-009318-zt1o1bcz	482	27	of	of	IN
cord-009318-zt1o1bcz	482	28	hgDNA	hgdna	JJ
cord-009318-zt1o1bcz	482	29	concentration	concentration	NN
cord-009318-zt1o1bcz	482	30	(	(	-LRB-
cord-009318-zt1o1bcz	482	31	Figure	figure	NN
cord-009318-zt1o1bcz	482	32	9B	9b	NN
cord-009318-zt1o1bcz	482	33	and	and	CC
cord-009318-zt1o1bcz	482	34	C	C	NNP
cord-009318-zt1o1bcz	482	35	)	)	-RRB-
cord-009318-zt1o1bcz	482	36	.	.	.
cord-009318-zt1o1bcz	483	1	The	the	DT
cord-009318-zt1o1bcz	483	2	fraction	fraction	NN
cord-009318-zt1o1bcz	483	3	of	of	IN
cord-009318-zt1o1bcz	483	4	partitions	partition	NNS
cord-009318-zt1o1bcz	483	5	generating	generate	VBG
cord-009318-zt1o1bcz	483	6	a	a	DT
cord-009318-zt1o1bcz	483	7	false	false	JJ
cord-009318-zt1o1bcz	483	8	-	-	HYPH
cord-009318-zt1o1bcz	483	9	positive	positive	JJ
cord-009318-zt1o1bcz	483	10	amplification	amplification	NN
cord-009318-zt1o1bcz	483	11	at	at	IN
cord-009318-zt1o1bcz	483	12	low	low	JJ
cord-009318-zt1o1bcz	483	13	T	t	NN
cord-009318-zt1o1bcz	483	14	m	m	NN
cord-009318-zt1o1bcz	483	15	was	be	VBD
cord-009318-zt1o1bcz	483	16	less	less	JJR
cord-009318-zt1o1bcz	483	17	than	than	IN
cord-009318-zt1o1bcz	483	18	3.3	3.3	CD
cord-009318-zt1o1bcz	483	19	×	×	NN
cord-009318-zt1o1bcz	483	20	10	10	CD
cord-009318-zt1o1bcz	483	21	−4	−4	CD
cord-009318-zt1o1bcz	483	22	through	through	IN
cord-009318-zt1o1bcz	483	23	45	45	CD
cord-009318-zt1o1bcz	483	24	min	min	NN
cord-009318-zt1o1bcz	483	25	(	(	-LRB-
cord-009318-zt1o1bcz	483	26	i.e.	i.e.	FW
cord-009318-zt1o1bcz	483	27	7	7	CD
cord-009318-zt1o1bcz	483	28	or	or	CC
cord-009318-zt1o1bcz	483	29	fewer	few	JJR
cord-009318-zt1o1bcz	483	30	events	event	NNS
cord-009318-zt1o1bcz	483	31	in	in	IN
cord-009318-zt1o1bcz	483	32	20,000	20,000	CD
cord-009318-zt1o1bcz	483	33	partitions	partition	NNS
cord-009318-zt1o1bcz	483	34	per	per	IN
cord-009318-zt1o1bcz	483	35	chip	chip	NN
cord-009318-zt1o1bcz	483	36	)	)	-RRB-
cord-009318-zt1o1bcz	483	37	.	.	.
cord-009318-zt1o1bcz	484	1	Similarly	similarly	RB
cord-009318-zt1o1bcz	484	2	,	,	,
cord-009318-zt1o1bcz	484	3	partition	partition	NN
cord-009318-zt1o1bcz	484	4	counts	count	NNS
cord-009318-zt1o1bcz	484	5	of	of	IN
cord-009318-zt1o1bcz	484	6	high	high	JJ
cord-009318-zt1o1bcz	484	7	-	-	HYPH
cord-009318-zt1o1bcz	484	8	T	t	NN
cord-009318-zt1o1bcz	484	9	m	m	NN
cord-009318-zt1o1bcz	484	10	non	non	JJ
cord-009318-zt1o1bcz	484	11	-	-	JJ
cord-009318-zt1o1bcz	484	12	specific	specific	JJ
cord-009318-zt1o1bcz	484	13	amplification	amplification	NN
cord-009318-zt1o1bcz	484	14	are	be	VBP
cord-009318-zt1o1bcz	484	15	<	<	XX
cord-009318-zt1o1bcz	484	16	10	10	CD
cord-009318-zt1o1bcz	484	17	per	per	IN
cord-009318-zt1o1bcz	484	18	chip	chip	NN
cord-009318-zt1o1bcz	484	19	until	until	IN
cord-009318-zt1o1bcz	484	20	45	45	CD
cord-009318-zt1o1bcz	484	21	min	min	NN
cord-009318-zt1o1bcz	484	22	.	.	.
cord-009318-zt1o1bcz	485	1	After	after	IN
cord-009318-zt1o1bcz	485	2	90	90	CD
cord-009318-zt1o1bcz	485	3	min	min	NN
cord-009318-zt1o1bcz	485	4	,	,	,
cord-009318-zt1o1bcz	485	5	high	high	JJ
cord-009318-zt1o1bcz	485	6	-	-	HYPH
cord-009318-zt1o1bcz	485	7	T	t	NN
cord-009318-zt1o1bcz	485	8	m	m	NN
cord-009318-zt1o1bcz	485	9	non	non	JJ
cord-009318-zt1o1bcz	485	10	-	-	JJ
cord-009318-zt1o1bcz	485	11	specific	specific	JJ
cord-009318-zt1o1bcz	485	12	amplification	amplification	NN
cord-009318-zt1o1bcz	485	13	is	be	VBZ
cord-009318-zt1o1bcz	485	14	more	more	RBR
cord-009318-zt1o1bcz	485	15	prevalent	prevalent	JJ
cord-009318-zt1o1bcz	485	16	than	than	IN
cord-009318-zt1o1bcz	485	17	low	low	JJ
cord-009318-zt1o1bcz	485	18	-	-	HYPH
cord-009318-zt1o1bcz	485	19	T	t	NN
cord-009318-zt1o1bcz	485	20	m	m	NN
cord-009318-zt1o1bcz	485	21	non	non	JJ
cord-009318-zt1o1bcz	485	22	-	-	JJ
cord-009318-zt1o1bcz	485	23	specific	specific	JJ
cord-009318-zt1o1bcz	485	24	amplification	amplification	NN
cord-009318-zt1o1bcz	485	25	and	and	CC
cord-009318-zt1o1bcz	485	26	the	the	DT
cord-009318-zt1o1bcz	485	27	reactions	reaction	NNS
cord-009318-zt1o1bcz	485	28	finish	finish	VBP
cord-009318-zt1o1bcz	485	29	with	with	IN
cord-009318-zt1o1bcz	485	30	fewer	few	JJR
cord-009318-zt1o1bcz	485	31	than	than	IN
cord-009318-zt1o1bcz	485	32	non	non	JJ
cord-009318-zt1o1bcz	485	33	-	-	JJ
cord-009318-zt1o1bcz	485	34	specific	specific	JJ
cord-009318-zt1o1bcz	485	35	260	260	CD
cord-009318-zt1o1bcz	485	36	counts	count	NNS
cord-009318-zt1o1bcz	485	37	in	in	IN
cord-009318-zt1o1bcz	485	38	20,000	20,000	CD
cord-009318-zt1o1bcz	485	39	partitions	partition	NNS
cord-009318-zt1o1bcz	485	40	corresponding	correspond	VBG
cord-009318-zt1o1bcz	485	41	to	to	IN
cord-009318-zt1o1bcz	485	42	a	a	DT
cord-009318-zt1o1bcz	485	43	false	false	JJ
cord-009318-zt1o1bcz	485	44	-	-	HYPH
cord-009318-zt1o1bcz	485	45	positive	positive	JJ
cord-009318-zt1o1bcz	485	46	fraction	fraction	NN
cord-009318-zt1o1bcz	485	47	of	of	IN
cord-009318-zt1o1bcz	485	48	1.3	1.3	CD
cord-009318-zt1o1bcz	485	49	×	×	CD
cord-009318-zt1o1bcz	485	50	10	10	CD
cord-009318-zt1o1bcz	485	51	−2	−2	NN
cord-009318-zt1o1bcz	485	52	.	.	.
cord-009318-zt1o1bcz	486	1	One	one	CD
cord-009318-zt1o1bcz	486	2	exception	exception	NN
cord-009318-zt1o1bcz	486	3	is	be	VBZ
cord-009318-zt1o1bcz	486	4	the	the	DT
cord-009318-zt1o1bcz	486	5	non	non	JJ
cord-009318-zt1o1bcz	486	6	-	-	JJ
cord-009318-zt1o1bcz	486	7	specific	specific	JJ
cord-009318-zt1o1bcz	486	8	high	high	JJ
cord-009318-zt1o1bcz	486	9	-	-	HYPH
cord-009318-zt1o1bcz	486	10	T	t	NN
cord-009318-zt1o1bcz	486	11	m	m	NN
cord-009318-zt1o1bcz	486	12	amplification	amplification	NN
cord-009318-zt1o1bcz	486	13	in	in	IN
cord-009318-zt1o1bcz	486	14	the	the	DT
cord-009318-zt1o1bcz	486	15	absence	absence	NN
cord-009318-zt1o1bcz	486	16	template	template	NN
cord-009318-zt1o1bcz	486	17	and	and	CC
cord-009318-zt1o1bcz	486	18	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	486	19	.	.	.
cord-009318-zt1o1bcz	487	1	This	this	DT
cord-009318-zt1o1bcz	487	2	condition	condition	NN
cord-009318-zt1o1bcz	487	3	appears	appear	VBZ
cord-009318-zt1o1bcz	487	4	to	to	TO
cord-009318-zt1o1bcz	487	5	have	have	VB
cord-009318-zt1o1bcz	487	6	lower	low	JJR
cord-009318-zt1o1bcz	487	7	non	non	JJ
cord-009318-zt1o1bcz	487	8	-	-	JJ
cord-009318-zt1o1bcz	487	9	specific	specific	JJ
cord-009318-zt1o1bcz	487	10	background	background	NN
cord-009318-zt1o1bcz	487	11	than	than	IN
cord-009318-zt1o1bcz	487	12	other	other	JJ
cord-009318-zt1o1bcz	487	13	conditions	condition	NNS
cord-009318-zt1o1bcz	487	14	.	.	.
cord-009318-zt1o1bcz	488	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	488	2	collected	collect	VBD
cord-009318-zt1o1bcz	488	3	each	each	DT
cord-009318-zt1o1bcz	488	4	replicate	replicate	VBP
cord-009318-zt1o1bcz	488	5	on	on	IN
cord-009318-zt1o1bcz	488	6	separate	separate	JJ
cord-009318-zt1o1bcz	488	7	days	day	NNS
cord-009318-zt1o1bcz	488	8	and	and	CC
cord-009318-zt1o1bcz	488	9	are	be	VBP
cord-009318-zt1o1bcz	488	10	able	able	JJ
cord-009318-zt1o1bcz	488	11	to	to	TO
cord-009318-zt1o1bcz	488	12	observe	observe	VB
cord-009318-zt1o1bcz	488	13	the	the	DT
cord-009318-zt1o1bcz	488	14	experimental	experimental	JJ
cord-009318-zt1o1bcz	488	15	variability	variability	NN
cord-009318-zt1o1bcz	488	16	between	between	IN
cord-009318-zt1o1bcz	488	17	the	the	DT
cord-009318-zt1o1bcz	488	18	presence	presence	NN
cord-009318-zt1o1bcz	488	19	and	and	CC
cord-009318-zt1o1bcz	488	20	absence	absence	NN
cord-009318-zt1o1bcz	488	21	fo	fo	IN
cord-009318-zt1o1bcz	488	22	template	template	NN
cord-009318-zt1o1bcz	488	23	,	,	,
cord-009318-zt1o1bcz	488	24	which	which	WDT
cord-009318-zt1o1bcz	488	25	might	may	MD
cord-009318-zt1o1bcz	488	26	be	be	VB
cord-009318-zt1o1bcz	488	27	otherwise	otherwise	RB
cord-009318-zt1o1bcz	488	28	lost	lose	VBN
cord-009318-zt1o1bcz	488	29	when	when	WRB
cord-009318-zt1o1bcz	488	30	examining	examine	VBG
cord-009318-zt1o1bcz	488	31	the	the	DT
cord-009318-zt1o1bcz	488	32	NTC	NTC	NNP
cord-009318-zt1o1bcz	488	33	alone	alone	RB
cord-009318-zt1o1bcz	488	34	.	.	.
cord-009318-zt1o1bcz	489	1	This	this	DT
cord-009318-zt1o1bcz	489	2	experiment	experiment	NN
cord-009318-zt1o1bcz	489	3	emphasizes	emphasize	VBZ
cord-009318-zt1o1bcz	489	4	the	the	DT
cord-009318-zt1o1bcz	489	5	advantage	advantage	NN
cord-009318-zt1o1bcz	489	6	of	of	IN
cord-009318-zt1o1bcz	489	7	determining	determine	VBG
cord-009318-zt1o1bcz	489	8	non	non	JJ
cord-009318-zt1o1bcz	489	9	-	-	JJ
cord-009318-zt1o1bcz	489	10	specific	specific	JJ
cord-009318-zt1o1bcz	489	11	amplificaiton	amplificaiton	NN
cord-009318-zt1o1bcz	489	12	using	use	VBG
cord-009318-zt1o1bcz	489	13	T	T	NNP
cord-009318-zt1o1bcz	489	14	m	m	NNP
cord-009318-zt1o1bcz	489	15	from	from	IN
cord-009318-zt1o1bcz	489	16	the	the	DT
cord-009318-zt1o1bcz	489	17	same	same	JJ
cord-009318-zt1o1bcz	489	18	experiment	experiment	NN
cord-009318-zt1o1bcz	489	19	as	as	IN
cord-009318-zt1o1bcz	489	20	specific	specific	JJ
cord-009318-zt1o1bcz	489	21	amplification	amplification	NN
cord-009318-zt1o1bcz	489	22	is	be	VBZ
cord-009318-zt1o1bcz	489	23	counted	count	VBN
cord-009318-zt1o1bcz	489	24	.	.	.
cord-009318-zt1o1bcz	490	1	At	at	IN
cord-009318-zt1o1bcz	490	2	low	low	JJ
cord-009318-zt1o1bcz	490	3	background	background	NN
cord-009318-zt1o1bcz	490	4	rates	rate	NNS
cord-009318-zt1o1bcz	490	5	,	,	,
cord-009318-zt1o1bcz	490	6	such	such	JJ
cord-009318-zt1o1bcz	490	7	as	as	IN
cord-009318-zt1o1bcz	490	8	when	when	WRB
cord-009318-zt1o1bcz	490	9	using	use	VBG
cord-009318-zt1o1bcz	490	10	Bst	Bst	NNP
cord-009318-zt1o1bcz	490	11	2.0	2.0	CD
cord-009318-zt1o1bcz	490	12	,	,	,
cord-009318-zt1o1bcz	490	13	inherant	inherant	JJ
cord-009318-zt1o1bcz	490	14	variability	variability	NN
cord-009318-zt1o1bcz	490	15	exists	exist	VBZ
cord-009318-zt1o1bcz	490	16	in	in	IN
cord-009318-zt1o1bcz	490	17	the	the	DT
cord-009318-zt1o1bcz	490	18	false	false	JJ
cord-009318-zt1o1bcz	490	19	-	-	HYPH
cord-009318-zt1o1bcz	490	20	positive	positive	JJ
cord-009318-zt1o1bcz	490	21	fraction	fraction	NN
cord-009318-zt1o1bcz	490	22	and	and	CC
cord-009318-zt1o1bcz	490	23	can	can	MD
cord-009318-zt1o1bcz	490	24	impact	impact	VB
cord-009318-zt1o1bcz	490	25	LOD	lod	NN
cord-009318-zt1o1bcz	490	26	.	.	.
cord-009318-zt1o1bcz	491	1	Measuring	measure	VBG
cord-009318-zt1o1bcz	491	2	non	non	JJ
cord-009318-zt1o1bcz	491	3	-	-	JJ
cord-009318-zt1o1bcz	491	4	specific	specific	JJ
cord-009318-zt1o1bcz	491	5	amplificaiton	amplificaiton	NN
cord-009318-zt1o1bcz	491	6	from	from	IN
cord-009318-zt1o1bcz	491	7	within	within	IN
cord-009318-zt1o1bcz	491	8	an	an	DT
cord-009318-zt1o1bcz	491	9	experimental	experimental	JJ
cord-009318-zt1o1bcz	491	10	eliminates	eliminate	VBZ
cord-009318-zt1o1bcz	491	11	the	the	DT
cord-009318-zt1o1bcz	491	12	assumption	assumption	NN
cord-009318-zt1o1bcz	491	13	that	that	IN
cord-009318-zt1o1bcz	491	14	the	the	DT
cord-009318-zt1o1bcz	491	15	false	false	JJ
cord-009318-zt1o1bcz	491	16	-	-	HYPH
cord-009318-zt1o1bcz	491	17	positive	positive	JJ
cord-009318-zt1o1bcz	491	18	rate	rate	NN
cord-009318-zt1o1bcz	491	19	remains	remain	VBZ
cord-009318-zt1o1bcz	491	20	identical	identical	JJ
cord-009318-zt1o1bcz	491	21	to	to	IN
cord-009318-zt1o1bcz	491	22	the	the	DT
cord-009318-zt1o1bcz	491	23	NTC	NTC	NNP
cord-009318-zt1o1bcz	491	24	or	or	CC
cord-009318-zt1o1bcz	491	25	between	between	IN
cord-009318-zt1o1bcz	491	26	experimental	experimental	JJ
cord-009318-zt1o1bcz	491	27	runs	run	NNS
cord-009318-zt1o1bcz	491	28	.	.	.
cord-009318-zt1o1bcz	492	1	For	for	IN
cord-009318-zt1o1bcz	492	2	Bst	Bst	NNP
cord-009318-zt1o1bcz	492	3	3.0	3.0	NNP
cord-009318-zt1o1bcz	492	4	,	,	,
cord-009318-zt1o1bcz	492	5	non	non	JJ
cord-009318-zt1o1bcz	492	6	-	-	JJ
cord-009318-zt1o1bcz	492	7	specific	specific	JJ
cord-009318-zt1o1bcz	492	8	amplification	amplification	NN
cord-009318-zt1o1bcz	492	9	was	be	VBD
cord-009318-zt1o1bcz	492	10	variable	variable	JJ
cord-009318-zt1o1bcz	492	11	,	,	,
cord-009318-zt1o1bcz	492	12	but	but	CC
cord-009318-zt1o1bcz	492	13	tended	tend	VBD
cord-009318-zt1o1bcz	492	14	to	to	TO
cord-009318-zt1o1bcz	492	15	be	be	VB
cord-009318-zt1o1bcz	492	16	fewer	few	JJR
cord-009318-zt1o1bcz	492	17	for	for	IN
cord-009318-zt1o1bcz	492	18	higher	high	JJR
cord-009318-zt1o1bcz	492	19	concentrations	concentration	NNS
cord-009318-zt1o1bcz	492	20	of	of	IN
cord-009318-zt1o1bcz	492	21	hgDNA	hgdna	NN
cord-009318-zt1o1bcz	492	22	.	.	.
cord-009318-zt1o1bcz	493	1	At	at	IN
cord-009318-zt1o1bcz	493	2	any	any	DT
cord-009318-zt1o1bcz	493	3	given	give	VBN
cord-009318-zt1o1bcz	493	4	time	time	NN
cord-009318-zt1o1bcz	493	5	,	,	,
cord-009318-zt1o1bcz	493	6	high	high	JJ
cord-009318-zt1o1bcz	493	7	-	-	HYPH
cord-009318-zt1o1bcz	493	8	T	t	NN
cord-009318-zt1o1bcz	493	9	m	m	NN
cord-009318-zt1o1bcz	493	10	non	non	JJ
cord-009318-zt1o1bcz	493	11	-	-	JJ
cord-009318-zt1o1bcz	493	12	specific	specific	JJ
cord-009318-zt1o1bcz	493	13	amplification	amplification	NN
cord-009318-zt1o1bcz	493	14	was	be	VBD
cord-009318-zt1o1bcz	493	15	on	on	IN
cord-009318-zt1o1bcz	493	16	average	average	JJ
cord-009318-zt1o1bcz	493	17	∼30-fold	∼30-fold	NN
cord-009318-zt1o1bcz	493	18	more	more	RBR
cord-009318-zt1o1bcz	493	19	likely	likely	JJ
cord-009318-zt1o1bcz	493	20	to	to	TO
cord-009318-zt1o1bcz	493	21	occur	occur	VB
cord-009318-zt1o1bcz	493	22	than	than	IN
cord-009318-zt1o1bcz	493	23	a	a	DT
cord-009318-zt1o1bcz	493	24	low	low	JJ
cord-009318-zt1o1bcz	493	25	-	-	HYPH
cord-009318-zt1o1bcz	493	26	T	t	NN
cord-009318-zt1o1bcz	493	27	m	m	NN
cord-009318-zt1o1bcz	493	28	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	493	29	product	product	NN
cord-009318-zt1o1bcz	493	30	.	.	.
cord-009318-zt1o1bcz	494	1	At	at	IN
cord-009318-zt1o1bcz	494	2	45	45	CD
cord-009318-zt1o1bcz	494	3	min	min	NN
cord-009318-zt1o1bcz	494	4	,	,	,
cord-009318-zt1o1bcz	494	5	low	low	JJ
cord-009318-zt1o1bcz	494	6	-	-	HYPH
cord-009318-zt1o1bcz	494	7	T	t	NN
cord-009318-zt1o1bcz	494	8	m	m	NN
cord-009318-zt1o1bcz	494	9	non	non	JJ
cord-009318-zt1o1bcz	494	10	-	-	JJ
cord-009318-zt1o1bcz	494	11	specific	specific	JJ
cord-009318-zt1o1bcz	494	12	amplification	amplification	NN
cord-009318-zt1o1bcz	494	13	had	have	VBD
cord-009318-zt1o1bcz	494	14	false	false	JJ
cord-009318-zt1o1bcz	494	15	-	-	HYPH
cord-009318-zt1o1bcz	494	16	positive	positive	JJ
cord-009318-zt1o1bcz	494	17	fraction	fraction	NN
cord-009318-zt1o1bcz	494	18	<	<	XX
cord-009318-zt1o1bcz	494	19	3.1	3.1	CD
cord-009318-zt1o1bcz	494	20	×	×	CD
cord-009318-zt1o1bcz	494	21	10	10	CD
cord-009318-zt1o1bcz	494	22	−3	−3	NN
cord-009318-zt1o1bcz	494	23	(	(	-LRB-
cord-009318-zt1o1bcz	494	24	62	62	CD
cord-009318-zt1o1bcz	494	25	or	or	CC
cord-009318-zt1o1bcz	494	26	fewer	few	JJR
cord-009318-zt1o1bcz	494	27	events	event	NNS
cord-009318-zt1o1bcz	494	28	per	per	IN
cord-009318-zt1o1bcz	494	29	chip	chip	NN
cord-009318-zt1o1bcz	494	30	)	)	-RRB-
cord-009318-zt1o1bcz	494	31	,	,	,
cord-009318-zt1o1bcz	494	32	amplification	amplification	NN
cord-009318-zt1o1bcz	494	33	events	event	NNS
cord-009318-zt1o1bcz	494	34	with	with	IN
cord-009318-zt1o1bcz	494	35	high	high	JJ
cord-009318-zt1o1bcz	494	36	T	t	NN
cord-009318-zt1o1bcz	494	37	m	m	NN
cord-009318-zt1o1bcz	494	38	had	have	VBD
cord-009318-zt1o1bcz	494	39	a	a	DT
cord-009318-zt1o1bcz	494	40	false	false	JJ
cord-009318-zt1o1bcz	494	41	-	-	HYPH
cord-009318-zt1o1bcz	494	42	positive	positive	JJ
cord-009318-zt1o1bcz	494	43	fraction	fraction	NN
cord-009318-zt1o1bcz	494	44	<	<	XX
cord-009318-zt1o1bcz	494	45	1.9	1.9	CD
cord-009318-zt1o1bcz	494	46	×	×	NN
cord-009318-zt1o1bcz	494	47	10	10	CD
cord-009318-zt1o1bcz	494	48	−2	−2	NNP
cord-009318-zt1o1bcz	494	49	(	(	-LRB-
cord-009318-zt1o1bcz	494	50	386	386	CD
cord-009318-zt1o1bcz	494	51	or	or	CC
cord-009318-zt1o1bcz	494	52	fewer	few	JJR
cord-009318-zt1o1bcz	494	53	events	event	NNS
cord-009318-zt1o1bcz	494	54	per	per	IN
cord-009318-zt1o1bcz	494	55	chip	chip	NN
cord-009318-zt1o1bcz	494	56	)	)	-RRB-
cord-009318-zt1o1bcz	494	57	.	.	.
cord-009318-zt1o1bcz	495	1	At	at	IN
cord-009318-zt1o1bcz	495	2	the	the	DT
cord-009318-zt1o1bcz	495	3	completion	completion	NN
cord-009318-zt1o1bcz	495	4	of	of	IN
cord-009318-zt1o1bcz	495	5	the	the	DT
cord-009318-zt1o1bcz	495	6	experiment	experiment	NN
cord-009318-zt1o1bcz	495	7	,	,	,
cord-009318-zt1o1bcz	495	8	high	high	JJ
cord-009318-zt1o1bcz	495	9	-	-	HYPH
cord-009318-zt1o1bcz	495	10	T	t	NN
cord-009318-zt1o1bcz	495	11	m	m	NN
cord-009318-zt1o1bcz	495	12	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	495	13	amplification	amplification	NN
cord-009318-zt1o1bcz	495	14	events	event	NNS
cord-009318-zt1o1bcz	495	15	account	account	VBP
cord-009318-zt1o1bcz	495	16	for	for	IN
cord-009318-zt1o1bcz	495	17	as	as	RB
cord-009318-zt1o1bcz	495	18	much	much	JJ
cord-009318-zt1o1bcz	495	19	as	as	IN
cord-009318-zt1o1bcz	495	20	35	35	CD
cord-009318-zt1o1bcz	495	21	%	%	NN
cord-009318-zt1o1bcz	495	22	of	of	IN
cord-009318-zt1o1bcz	495	23	the	the	DT
cord-009318-zt1o1bcz	495	24	total	total	JJ
cord-009318-zt1o1bcz	495	25	partitions	partition	NNS
cord-009318-zt1o1bcz	495	26	per	per	IN
cord-009318-zt1o1bcz	495	27	chip	chip	NN
cord-009318-zt1o1bcz	495	28	;	;	:
cord-009318-zt1o1bcz	495	29	a	a	DT
cord-009318-zt1o1bcz	495	30	value	value	NN
cord-009318-zt1o1bcz	495	31	exceeding	exceed	VBG
cord-009318-zt1o1bcz	495	32	the	the	DT
cord-009318-zt1o1bcz	495	33	total	total	JJ
cord-009318-zt1o1bcz	495	34	ob	ob	NNP
cord-009318-zt1o1bcz	495	35	-	-	HYPH
cord-009318-zt1o1bcz	495	36	served	serve	VBN
cord-009318-zt1o1bcz	495	37	true	true	JJ
cord-009318-zt1o1bcz	495	38	-	-	HYPH
cord-009318-zt1o1bcz	495	39	positive	positive	JJ
cord-009318-zt1o1bcz	495	40	events	event	NNS
cord-009318-zt1o1bcz	495	41	.	.	.
cord-009318-zt1o1bcz	496	1	In	in	IN
cord-009318-zt1o1bcz	496	2	these	these	DT
cord-009318-zt1o1bcz	496	3	scenarios	scenario	NNS
cord-009318-zt1o1bcz	496	4	,	,	,
cord-009318-zt1o1bcz	496	5	utilization	utilization	NN
cord-009318-zt1o1bcz	496	6	of	of	IN
cord-009318-zt1o1bcz	496	7	T	T	NNP
cord-009318-zt1o1bcz	496	8	m	m	NN
cord-009318-zt1o1bcz	496	9	to	to	TO
cord-009318-zt1o1bcz	496	10	identify	identify	VB
cord-009318-zt1o1bcz	496	11	true	true	JJ
cord-009318-zt1o1bcz	496	12	and	and	CC
cord-009318-zt1o1bcz	496	13	false	false	JJ
cord-009318-zt1o1bcz	496	14	amplification	amplification	NN
cord-009318-zt1o1bcz	496	15	will	will	MD
cord-009318-zt1o1bcz	496	16	be	be	VB
cord-009318-zt1o1bcz	496	17	critical	critical	JJ
cord-009318-zt1o1bcz	496	18	to	to	IN
cord-009318-zt1o1bcz	496	19	successful	successful	JJ
cord-009318-zt1o1bcz	496	20	quantification	quantification	NN
cord-009318-zt1o1bcz	496	21	of	of	IN
cord-009318-zt1o1bcz	496	22	target	target	NN
cord-009318-zt1o1bcz	496	23	analytes	analyte	NNS
cord-009318-zt1o1bcz	496	24	.	.	.
cord-009318-zt1o1bcz	497	1	For	for	IN
cord-009318-zt1o1bcz	497	2	this	this	DT
cord-009318-zt1o1bcz	497	3	CT	CT	NNP
cord-009318-zt1o1bcz	497	4	primer	primer	NN
cord-009318-zt1o1bcz	497	5	set	set	NN
cord-009318-zt1o1bcz	497	6	,	,	,
cord-009318-zt1o1bcz	497	7	both	both	DT
cord-009318-zt1o1bcz	497	8	Bst	Bst	NNP
cord-009318-zt1o1bcz	497	9	2.0	2.0	NNP
cord-009318-zt1o1bcz	497	10	and	and	CC
cord-009318-zt1o1bcz	497	11	Bst	Bst	NNP
cord-009318-zt1o1bcz	497	12	3.0	3.0	NNP
cord-009318-zt1o1bcz	497	13	similarly	similarly	RB
cord-009318-zt1o1bcz	497	14	demonstrate	demonstrate	VBP
cord-009318-zt1o1bcz	497	15	that	that	IN
cord-009318-zt1o1bcz	497	16	the	the	DT
cord-009318-zt1o1bcz	497	17	presence	presence	NN
cord-009318-zt1o1bcz	497	18	of	of	IN
cord-009318-zt1o1bcz	497	19	high	high	JJ
cord-009318-zt1o1bcz	497	20	concentrations	concentration	NNS
cord-009318-zt1o1bcz	497	21	of	of	IN
cord-009318-zt1o1bcz	497	22	hgDNA	hgDNA	VBG
cord-009318-zt1o1bcz	497	23	may	may	MD
cord-009318-zt1o1bcz	497	24	suppress	suppress	VB
cord-009318-zt1o1bcz	497	25	the	the	DT
cord-009318-zt1o1bcz	497	26	likelihood	likelihood	NN
cord-009318-zt1o1bcz	497	27	of	of	IN
cord-009318-zt1o1bcz	497	28	non	non	JJ
cord-009318-zt1o1bcz	497	29	-	-	JJ
cord-009318-zt1o1bcz	497	30	specific	specific	JJ
cord-009318-zt1o1bcz	497	31	amplification	amplification	NN
cord-009318-zt1o1bcz	497	32	occurring	occur	VBG
cord-009318-zt1o1bcz	497	33	.	.	.
cord-009318-zt1o1bcz	498	1	In	in	IN
cord-009318-zt1o1bcz	498	2	general	general	JJ
cord-009318-zt1o1bcz	498	3	,	,	,
cord-009318-zt1o1bcz	498	4	for	for	IN
cord-009318-zt1o1bcz	498	5	this	this	DT
cord-009318-zt1o1bcz	498	6	primer	primer	NN
cord-009318-zt1o1bcz	498	7	set	set	NN
cord-009318-zt1o1bcz	498	8	and	and	CC
cord-009318-zt1o1bcz	498	9	target	target	NN
cord-009318-zt1o1bcz	498	10	,	,	,
cord-009318-zt1o1bcz	498	11	we	-PRON-	PRP
cord-009318-zt1o1bcz	498	12	find	find	VBP
cord-009318-zt1o1bcz	498	13	that	that	IN
cord-009318-zt1o1bcz	498	14	Bst	Bst	NNP
cord-009318-zt1o1bcz	498	15	2.0	2.0	NNP
cord-009318-zt1o1bcz	498	16	performs	perform	VBZ
cord-009318-zt1o1bcz	498	17	significantly	significantly	RB
cord-009318-zt1o1bcz	498	18	better	well	JJR
cord-009318-zt1o1bcz	498	19	than	than	IN
cord-009318-zt1o1bcz	498	20	Bst	Bst	NNP
cord-009318-zt1o1bcz	498	21	3.0	3.0	NNP
cord-009318-zt1o1bcz	498	22	as	as	IN
cord-009318-zt1o1bcz	498	23	a	a	DT
cord-009318-zt1o1bcz	498	24	consequence	consequence	NN
cord-009318-zt1o1bcz	498	25	of	of	IN
cord-009318-zt1o1bcz	498	26	having	have	VBG
cord-009318-zt1o1bcz	498	27	higher	high	JJR
cord-009318-zt1o1bcz	498	28	probability	probability	NN
cord-009318-zt1o1bcz	498	29	of	of	IN
cord-009318-zt1o1bcz	498	30	detecting	detect	VBG
cord-009318-zt1o1bcz	498	31	a	a	DT
cord-009318-zt1o1bcz	498	32	target	target	NN
cord-009318-zt1o1bcz	498	33	molecule	molecule	NN
cord-009318-zt1o1bcz	498	34	and	and	CC
cord-009318-zt1o1bcz	498	35	low	low	JJ
cord-009318-zt1o1bcz	498	36	likelihood	likelihood	NN
cord-009318-zt1o1bcz	498	37	of	of	IN
cord-009318-zt1o1bcz	498	38	generating	generate	VBG
cord-009318-zt1o1bcz	498	39	a	a	DT
cord-009318-zt1o1bcz	498	40	non	non	JJ
cord-009318-zt1o1bcz	498	41	-	-	JJ
cord-009318-zt1o1bcz	498	42	specific	specific	JJ
cord-009318-zt1o1bcz	498	43	amplification	amplification	NN
cord-009318-zt1o1bcz	498	44	event	event	NN
cord-009318-zt1o1bcz	498	45	.	.	.
cord-009318-zt1o1bcz	499	1	Fourth	fourth	RB
cord-009318-zt1o1bcz	499	2	,	,	,
cord-009318-zt1o1bcz	499	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	499	4	asked	ask	VBD
cord-009318-zt1o1bcz	499	5	is	be	VBZ
cord-009318-zt1o1bcz	499	6	maximum	maximum	JJ
cord-009318-zt1o1bcz	499	7	rate	rate	NN
cord-009318-zt1o1bcz	499	8	impacted	impact	VBN
cord-009318-zt1o1bcz	499	9	by	by	IN
cord-009318-zt1o1bcz	499	10	the	the	DT
cord-009318-zt1o1bcz	499	11	concentration	concentration	NN
cord-009318-zt1o1bcz	499	12	of	of	IN
cord-009318-zt1o1bcz	499	13	hgDNA	hgdna	XX
cord-009318-zt1o1bcz	499	14	?	?	.
cord-009318-zt1o1bcz	500	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	500	2	hypothesize	hypothesize	VBP
cord-009318-zt1o1bcz	500	3	that	that	DT
cord-009318-zt1o1bcz	500	4	background	background	NN
cord-009318-zt1o1bcz	500	5	hgDNA	hgDNA	MD
cord-009318-zt1o1bcz	500	6	may	may	MD
cord-009318-zt1o1bcz	500	7	compete	compete	VB
cord-009318-zt1o1bcz	500	8	for	for	IN
cord-009318-zt1o1bcz	500	9	the	the	DT
cord-009318-zt1o1bcz	500	10	binding	binding	JJ
cord-009318-zt1o1bcz	500	11	site	site	NN
cord-009318-zt1o1bcz	500	12	of	of	IN
cord-009318-zt1o1bcz	500	13	the	the	DT
cord-009318-zt1o1bcz	500	14	polymerase	polymerase	NN
cord-009318-zt1o1bcz	500	15	with	with	IN
cord-009318-zt1o1bcz	500	16	the	the	DT
cord-009318-zt1o1bcz	500	17	target	target	NN
cord-009318-zt1o1bcz	500	18	DNA	dna	NN
cord-009318-zt1o1bcz	500	19	or	or	CC
cord-009318-zt1o1bcz	500	20	generate	generate	VB
cord-009318-zt1o1bcz	500	21	competing	compete	VBG
cord-009318-zt1o1bcz	500	22	amplification	amplification	NN
cord-009318-zt1o1bcz	500	23	events	event	NNS
cord-009318-zt1o1bcz	500	24	and	and	CC
cord-009318-zt1o1bcz	500	25	thus	thus	RB
cord-009318-zt1o1bcz	500	26	,	,	,
cord-009318-zt1o1bcz	500	27	decrease	decrease	VB
cord-009318-zt1o1bcz	500	28	the	the	DT
cord-009318-zt1o1bcz	500	29	maximum	maximum	JJ
cord-009318-zt1o1bcz	500	30	observed	observed	JJ
cord-009318-zt1o1bcz	500	31	velocity	velocity	NN
cord-009318-zt1o1bcz	500	32	in	in	IN
cord-009318-zt1o1bcz	500	33	a	a	DT
cord-009318-zt1o1bcz	500	34	given	give	VBN
cord-009318-zt1o1bcz	500	35	partition	partition	NN
cord-009318-zt1o1bcz	500	36	.	.	.
cord-009318-zt1o1bcz	501	1	This	this	DT
cord-009318-zt1o1bcz	501	2	phenomena	phenomena	NN
cord-009318-zt1o1bcz	501	3	would	would	MD
cord-009318-zt1o1bcz	501	4	be	be	VB
cord-009318-zt1o1bcz	501	5	challenging	challenge	VBG
cord-009318-zt1o1bcz	501	6	to	to	TO
cord-009318-zt1o1bcz	501	7	untangle	untangle	VB
cord-009318-zt1o1bcz	501	8	in	in	IN
cord-009318-zt1o1bcz	501	9	bulk	bulk	NN
cord-009318-zt1o1bcz	501	10	.	.	.
cord-009318-zt1o1bcz	502	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	502	2	find	find	VBP
cord-009318-zt1o1bcz	502	3	that	that	IN
cord-009318-zt1o1bcz	502	4	maximum	maximum	JJ
cord-009318-zt1o1bcz	502	5	rates	rate	NNS
cord-009318-zt1o1bcz	502	6	are	be	VBP
cord-009318-zt1o1bcz	502	7	similar	similar	JJ
cord-009318-zt1o1bcz	502	8	for	for	IN
cord-009318-zt1o1bcz	502	9	a	a	DT
cord-009318-zt1o1bcz	502	10	given	give	VBN
cord-009318-zt1o1bcz	502	11	enzyme	enzyme	NN
cord-009318-zt1o1bcz	502	12	,	,	,
cord-009318-zt1o1bcz	502	13	until	until	IN
cord-009318-zt1o1bcz	502	14	5000	5000	CD
cord-009318-zt1o1bcz	502	15	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	502	16	per	per	IN
cord-009318-zt1o1bcz	502	17	l	l	NN
cord-009318-zt1o1bcz	502	18	for	for	IN
cord-009318-zt1o1bcz	502	19	Bst	Bst	NNP
cord-009318-zt1o1bcz	502	20	2.0	2.0	CD
cord-009318-zt1o1bcz	502	21	(	(	-LRB-
cord-009318-zt1o1bcz	502	22	Supplementary	supplementary	JJ
cord-009318-zt1o1bcz	502	23	Figure	figure	NN
cord-009318-zt1o1bcz	502	24	S11A	s11a	NN
cord-009318-zt1o1bcz	502	25	)	)	-RRB-
cord-009318-zt1o1bcz	502	26	and	and	CC
cord-009318-zt1o1bcz	502	27	above	above	IN
cord-009318-zt1o1bcz	502	28	100	100	CD
cord-009318-zt1o1bcz	502	29	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	502	30	per	per	IN
cord-009318-zt1o1bcz	502	31	l	l	NN
cord-009318-zt1o1bcz	502	32	for	for	IN
cord-009318-zt1o1bcz	502	33	Bst	Bst	NNP
cord-009318-zt1o1bcz	502	34	3.0	3.0	NNP
cord-009318-zt1o1bcz	502	35	(	(	-LRB-
cord-009318-zt1o1bcz	502	36	Supplementary	supplementary	JJ
cord-009318-zt1o1bcz	502	37	Figure	figure	NN
cord-009318-zt1o1bcz	502	38	S11B	S11B	NNS
cord-009318-zt1o1bcz	502	39	)	)	-RRB-
cord-009318-zt1o1bcz	502	40	.	.	.
cord-009318-zt1o1bcz	503	1	Thus	thus	RB
cord-009318-zt1o1bcz	503	2	demonstrating	demonstrate	VBG
cord-009318-zt1o1bcz	503	3	that	that	IN
cord-009318-zt1o1bcz	503	4	high	high	JJ
cord-009318-zt1o1bcz	503	5	concentrations	concentration	NNS
cord-009318-zt1o1bcz	503	6	of	of	IN
cord-009318-zt1o1bcz	503	7	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	503	8	may	may	MD
cord-009318-zt1o1bcz	503	9	slow	slow	VB
cord-009318-zt1o1bcz	503	10	the	the	DT
cord-009318-zt1o1bcz	503	11	rate	rate	NN
cord-009318-zt1o1bcz	503	12	of	of	IN
cord-009318-zt1o1bcz	503	13	amplification	amplification	NN
cord-009318-zt1o1bcz	503	14	.	.	.
cord-009318-zt1o1bcz	504	1	Furthermore	furthermore	RB
cord-009318-zt1o1bcz	504	2	,	,	,
cord-009318-zt1o1bcz	504	3	in	in	IN
cord-009318-zt1o1bcz	504	4	general	general	JJ
cord-009318-zt1o1bcz	504	5	,	,	,
cord-009318-zt1o1bcz	504	6	and	and	CC
cord-009318-zt1o1bcz	504	7	echoing	echo	VBG
cord-009318-zt1o1bcz	504	8	the	the	DT
cord-009318-zt1o1bcz	504	9	conclusions	conclusion	NNS
cord-009318-zt1o1bcz	504	10	of	of	IN
cord-009318-zt1o1bcz	504	11	Figure	figure	NN
cord-009318-zt1o1bcz	504	12	6	6	CD
cord-009318-zt1o1bcz	504	13	G	g	NN
cord-009318-zt1o1bcz	504	14	and	and	CC
cord-009318-zt1o1bcz	504	15	I	-PRON-	PRP
cord-009318-zt1o1bcz	504	16	,	,	,
cord-009318-zt1o1bcz	504	17	we	-PRON-	PRP
cord-009318-zt1o1bcz	504	18	observe	observe	VBP
cord-009318-zt1o1bcz	504	19	that	that	IN
cord-009318-zt1o1bcz	504	20	Bst	Bst	NNP
cord-009318-zt1o1bcz	504	21	2.0	2.0	NNP
cord-009318-zt1o1bcz	504	22	has	have	VBZ
cord-009318-zt1o1bcz	504	23	faster	fast	JJR
cord-009318-zt1o1bcz	504	24	maximum	maximum	JJ
cord-009318-zt1o1bcz	504	25	rate	rate	NN
cord-009318-zt1o1bcz	504	26	than	than	IN
cord-009318-zt1o1bcz	504	27	Bst	Bst	NNP
cord-009318-zt1o1bcz	504	28	3.0	3.0	CD
cord-009318-zt1o1bcz	504	29	,	,	,
cord-009318-zt1o1bcz	504	30	regardless	regardless	RB
cord-009318-zt1o1bcz	504	31	of	of	IN
cord-009318-zt1o1bcz	504	32	the	the	DT
cord-009318-zt1o1bcz	504	33	hgDNA	hgdna	JJ
cord-009318-zt1o1bcz	504	34	concentration	concentration	NN
cord-009318-zt1o1bcz	504	35	.	.	.
cord-009318-zt1o1bcz	505	1	Fifth	fifth	JJ
cord-009318-zt1o1bcz	505	2	,	,	,
cord-009318-zt1o1bcz	505	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	505	4	asked	ask	VBD
cord-009318-zt1o1bcz	505	5	how	how	WRB
cord-009318-zt1o1bcz	505	6	is	be	VBZ
cord-009318-zt1o1bcz	505	7	LOD	lod	NN
cord-009318-zt1o1bcz	505	8	impacted	impact	VBN
cord-009318-zt1o1bcz	505	9	by	by	IN
cord-009318-zt1o1bcz	505	10	the	the	DT
cord-009318-zt1o1bcz	505	11	concentration	concentration	NN
cord-009318-zt1o1bcz	505	12	of	of	IN
cord-009318-zt1o1bcz	505	13	hgDNA	hgdna	XX
cord-009318-zt1o1bcz	505	14	?	?	.
cord-009318-zt1o1bcz	506	1	For	for	IN
cord-009318-zt1o1bcz	506	2	Bst	Bst	NNP
cord-009318-zt1o1bcz	506	3	2.0	2.0	CD
cord-009318-zt1o1bcz	506	4	(	(	-LRB-
cord-009318-zt1o1bcz	506	5	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	506	6	Figure	Figure	NNP
cord-009318-zt1o1bcz	506	7	S11E	S11E	NNP
cord-009318-zt1o1bcz	506	8	)	)	-RRB-
cord-009318-zt1o1bcz	506	9	,	,	,
cord-009318-zt1o1bcz	506	10	the	the	DT
cord-009318-zt1o1bcz	506	11	LOD	lod	NN
cord-009318-zt1o1bcz	506	12	at	at	IN
cord-009318-zt1o1bcz	506	13	a	a	DT
cord-009318-zt1o1bcz	506	14	given	give	VBN
cord-009318-zt1o1bcz	506	15	time	time	NN
cord-009318-zt1o1bcz	506	16	was	be	VBD
cord-009318-zt1o1bcz	506	17	similar	similar	JJ
cord-009318-zt1o1bcz	506	18	for	for	IN
cord-009318-zt1o1bcz	506	19	concentrations	concentration	NNS
cord-009318-zt1o1bcz	506	20	<	<	XX
cord-009318-zt1o1bcz	506	21	5000	5000	CD
cord-009318-zt1o1bcz	506	22	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	506	23	per	per	IN
cord-009318-zt1o1bcz	506	24	l.	l.	NN
cord-009318-zt1o1bcz	507	1	While	while	IN
cord-009318-zt1o1bcz	507	2	the	the	DT
cord-009318-zt1o1bcz	507	3	LOD	lod	NN
cord-009318-zt1o1bcz	507	4	in	in	IN
cord-009318-zt1o1bcz	507	5	the	the	DT
cord-009318-zt1o1bcz	507	6	presence	presence	NN
cord-009318-zt1o1bcz	507	7	of	of	IN
cord-009318-zt1o1bcz	507	8	5000	5000	CD
cord-009318-zt1o1bcz	507	9	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	507	10	per	per	IN
cord-009318-zt1o1bcz	507	11	l	l	NN
cord-009318-zt1o1bcz	507	12	was	be	VBD
cord-009318-zt1o1bcz	507	13	slightly	slightly	RB
cord-009318-zt1o1bcz	507	14	worse	bad	JJR
cord-009318-zt1o1bcz	507	15	from	from	IN
cord-009318-zt1o1bcz	507	16	the	the	DT
cord-009318-zt1o1bcz	507	17	detection	detection	NN
cord-009318-zt1o1bcz	507	18	of	of	IN
cord-009318-zt1o1bcz	507	19	fewer	few	JJR
cord-009318-zt1o1bcz	507	20	target	target	NN
cord-009318-zt1o1bcz	507	21	molecules	molecule	NNS
cord-009318-zt1o1bcz	507	22	(	(	-LRB-
cord-009318-zt1o1bcz	507	23	e.g.	e.g.	RB
cord-009318-zt1o1bcz	507	24	0.7	0.7	CD
cord-009318-zt1o1bcz	507	25	versus	versus	IN
cord-009318-zt1o1bcz	507	26	0.5	0.5	CD
cord-009318-zt1o1bcz	507	27	cp	cp	NN
cord-009318-zt1o1bcz	507	28	/	/	CC
cord-009318-zt1o1bcz	507	29	l	l	NN
cord-009318-zt1o1bcz	507	30	at	at	IN
cord-009318-zt1o1bcz	507	31	45	45	CD
cord-009318-zt1o1bcz	507	32	min	min	NN
cord-009318-zt1o1bcz	507	33	)	)	-RRB-
cord-009318-zt1o1bcz	507	34	.	.	.
cord-009318-zt1o1bcz	508	1	As	as	IN
cord-009318-zt1o1bcz	508	2	previously	previously	RB
cord-009318-zt1o1bcz	508	3	,	,	,
cord-009318-zt1o1bcz	508	4	incorporation	incorporation	NN
cord-009318-zt1o1bcz	508	5	of	of	IN
cord-009318-zt1o1bcz	508	6	HRM	HRM	NNP
cord-009318-zt1o1bcz	508	7	into	into	IN
cord-009318-zt1o1bcz	508	8	the	the	DT
cord-009318-zt1o1bcz	508	9	final	final	JJ
cord-009318-zt1o1bcz	508	10	assay	assay	NN
cord-009318-zt1o1bcz	508	11	does	do	VBZ
cord-009318-zt1o1bcz	508	12	not	not	RB
cord-009318-zt1o1bcz	508	13	impact	impact	VB
cord-009318-zt1o1bcz	508	14	the	the	DT
cord-009318-zt1o1bcz	508	15	LOD	lod	NN
cord-009318-zt1o1bcz	508	16	when	when	WRB
cord-009318-zt1o1bcz	508	17	using	use	VBG
cord-009318-zt1o1bcz	508	18	Bst	Bst	NNP
cord-009318-zt1o1bcz	508	19	2.0	2.0	NNP
cord-009318-zt1o1bcz	508	20	.	.	.
cord-009318-zt1o1bcz	509	1	When	when	WRB
cord-009318-zt1o1bcz	509	2	using	use	VBG
cord-009318-zt1o1bcz	509	3	Bst	Bst	NNP
cord-009318-zt1o1bcz	509	4	3.0	3.0	NNP
cord-009318-zt1o1bcz	509	5	(	(	-LRB-
cord-009318-zt1o1bcz	509	6	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	509	7	Figure	Figure	NNP
cord-009318-zt1o1bcz	509	8	S11F	S11F	NNP
cord-009318-zt1o1bcz	509	9	)	)	-RRB-
cord-009318-zt1o1bcz	509	10	and	and	CC
cord-009318-zt1o1bcz	509	11	HRM	HRM	NNP
cord-009318-zt1o1bcz	509	12	to	to	TO
cord-009318-zt1o1bcz	509	13	remove	remove	VB
cord-009318-zt1o1bcz	509	14	non	non	JJ
cord-009318-zt1o1bcz	509	15	-	-	JJ
cord-009318-zt1o1bcz	509	16	specific	specific	JJ
cord-009318-zt1o1bcz	509	17	amplification	amplification	NN
cord-009318-zt1o1bcz	509	18	,	,	,
cord-009318-zt1o1bcz	509	19	LOD	lod	NN
cord-009318-zt1o1bcz	509	20	tracks	track	NNS
cord-009318-zt1o1bcz	509	21	with	with	IN
cord-009318-zt1o1bcz	509	22	the	the	DT
cord-009318-zt1o1bcz	509	23	number	number	NN
cord-009318-zt1o1bcz	509	24	of	of	IN
cord-009318-zt1o1bcz	509	25	true	true	JJ
cord-009318-zt1o1bcz	509	26	-	-	HYPH
cord-009318-zt1o1bcz	509	27	positive	positive	JJ
cord-009318-zt1o1bcz	509	28	events	event	NNS
cord-009318-zt1o1bcz	509	29	.	.	.
cord-009318-zt1o1bcz	510	1	Thus	thus	RB
cord-009318-zt1o1bcz	510	2	,	,	,
cord-009318-zt1o1bcz	510	3	LOD	LOD	NNP
cord-009318-zt1o1bcz	510	4	becomes	become	VBZ
cord-009318-zt1o1bcz	510	5	worse	bad	JJR
cord-009318-zt1o1bcz	510	6	when	when	WRB
cord-009318-zt1o1bcz	510	7	efficiency	efficiency	NN
cord-009318-zt1o1bcz	510	8	is	be	VBZ
cord-009318-zt1o1bcz	510	9	lower	low	JJR
cord-009318-zt1o1bcz	510	10	(	(	-LRB-
cord-009318-zt1o1bcz	510	11	i.e.	i.e.	FW
cord-009318-zt1o1bcz	510	12	at	at	IN
cord-009318-zt1o1bcz	510	13	5000	5000	CD
cord-009318-zt1o1bcz	510	14	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	510	15	per	per	IN
cord-009318-zt1o1bcz	510	16	l	l	NN
cord-009318-zt1o1bcz	510	17	)	)	-RRB-
cord-009318-zt1o1bcz	510	18	.	.	.
cord-009318-zt1o1bcz	511	1	Similarly	similarly	RB
cord-009318-zt1o1bcz	511	2	,	,	,
cord-009318-zt1o1bcz	511	3	when	when	WRB
cord-009318-zt1o1bcz	511	4	HRM	HRM	NNP
cord-009318-zt1o1bcz	511	5	is	be	VBZ
cord-009318-zt1o1bcz	511	6	not	not	RB
cord-009318-zt1o1bcz	511	7	incorporated	incorporate	VBN
cord-009318-zt1o1bcz	511	8	in	in	IN
cord-009318-zt1o1bcz	511	9	the	the	DT
cord-009318-zt1o1bcz	511	10	assay	assay	NN
cord-009318-zt1o1bcz	511	11	,	,	,
cord-009318-zt1o1bcz	511	12	higher	high	JJR
cord-009318-zt1o1bcz	511	13	concentrations	concentration	NNS
cord-009318-zt1o1bcz	511	14	of	of	IN
cord-009318-zt1o1bcz	511	15	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	511	16	tend	tend	VBP
cord-009318-zt1o1bcz	511	17	to	to	TO
cord-009318-zt1o1bcz	511	18	result	result	VB
cord-009318-zt1o1bcz	511	19	in	in	IN
cord-009318-zt1o1bcz	511	20	a	a	DT
cord-009318-zt1o1bcz	511	21	worse	bad	JJR
cord-009318-zt1o1bcz	511	22	LOD	lod	NN
cord-009318-zt1o1bcz	511	23	.	.	.
cord-009318-zt1o1bcz	512	1	However	however	RB
cord-009318-zt1o1bcz	512	2	,	,	,
cord-009318-zt1o1bcz	512	3	at	at	IN
cord-009318-zt1o1bcz	512	4	long	long	JJ
cord-009318-zt1o1bcz	512	5	amplification	amplification	NN
cord-009318-zt1o1bcz	512	6	times	time	NNS
cord-009318-zt1o1bcz	512	7	,	,	,
cord-009318-zt1o1bcz	512	8	high	high	JJ
cord-009318-zt1o1bcz	512	9	concentrations	concentration	NNS
cord-009318-zt1o1bcz	512	10	of	of	IN
cord-009318-zt1o1bcz	512	11	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	512	12	suppress	suppress	VBP
cord-009318-zt1o1bcz	512	13	non	non	JJ
cord-009318-zt1o1bcz	512	14	-	-	JJ
cord-009318-zt1o1bcz	512	15	specific	specific	JJ
cord-009318-zt1o1bcz	512	16	amplification	amplification	NN
cord-009318-zt1o1bcz	512	17	more	more	RBR
cord-009318-zt1o1bcz	512	18	than	than	IN
cord-009318-zt1o1bcz	512	19	specific	specific	JJ
cord-009318-zt1o1bcz	512	20	amplification	amplification	NN
cord-009318-zt1o1bcz	512	21	,	,	,
cord-009318-zt1o1bcz	512	22	resulting	result	VBG
cord-009318-zt1o1bcz	512	23	in	in	IN
cord-009318-zt1o1bcz	512	24	LOD	lod	NN
cord-009318-zt1o1bcz	512	25	enhancement	enhancement	NN
cord-009318-zt1o1bcz	512	26	relative	relative	JJ
cord-009318-zt1o1bcz	512	27	to	to	IN
cord-009318-zt1o1bcz	512	28	low	low	JJ
cord-009318-zt1o1bcz	512	29	concentrations	concentration	NNS
cord-009318-zt1o1bcz	512	30	of	of	IN
cord-009318-zt1o1bcz	512	31	HHGE	HHGE	NNP
cord-009318-zt1o1bcz	512	32	.	.	.
cord-009318-zt1o1bcz	513	1	Cumulatively	cumulatively	RB
cord-009318-zt1o1bcz	513	2	,	,	,
cord-009318-zt1o1bcz	513	3	these	these	DT
cord-009318-zt1o1bcz	513	4	data	datum	NNS
cord-009318-zt1o1bcz	513	5	show	show	VBP
cord-009318-zt1o1bcz	513	6	high	high	JJ
cord-009318-zt1o1bcz	513	7	background	background	NN
cord-009318-zt1o1bcz	513	8	DNA	DNA	NNP
cord-009318-zt1o1bcz	513	9	may	may	MD
cord-009318-zt1o1bcz	513	10	reduce	reduce	VB
cord-009318-zt1o1bcz	513	11	the	the	DT
cord-009318-zt1o1bcz	513	12	probability	probability	NN
cord-009318-zt1o1bcz	513	13	of	of	IN
cord-009318-zt1o1bcz	513	14	detecting	detect	VBG
cord-009318-zt1o1bcz	513	15	a	a	DT
cord-009318-zt1o1bcz	513	16	specific	specific	JJ
cord-009318-zt1o1bcz	513	17	molecule	molecule	NN
cord-009318-zt1o1bcz	513	18	(	(	-LRB-
cord-009318-zt1o1bcz	513	19	analytical	analytical	JJ
cord-009318-zt1o1bcz	513	20	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	513	21	)	)	-RRB-
cord-009318-zt1o1bcz	513	22	,	,	,
cord-009318-zt1o1bcz	513	23	suppress	suppress	VB
cord-009318-zt1o1bcz	513	24	the	the	DT
cord-009318-zt1o1bcz	513	25	false	false	JJ
cord-009318-zt1o1bcz	513	26	-	-	HYPH
cord-009318-zt1o1bcz	513	27	positive	positive	JJ
cord-009318-zt1o1bcz	513	28	fraction	fraction	NN
cord-009318-zt1o1bcz	513	29	(	(	-LRB-
cord-009318-zt1o1bcz	513	30	analytical	analytical	JJ
cord-009318-zt1o1bcz	513	31	specificity	specificity	NN
cord-009318-zt1o1bcz	513	32	)	)	-RRB-
cord-009318-zt1o1bcz	513	33	,	,	,
cord-009318-zt1o1bcz	513	34	reduce	reduce	VB
cord-009318-zt1o1bcz	513	35	the	the	DT
cord-009318-zt1o1bcz	513	36	velocity	velocity	NN
cord-009318-zt1o1bcz	513	37	of	of	IN
cord-009318-zt1o1bcz	513	38	amplification	amplification	NN
cord-009318-zt1o1bcz	513	39	,	,	,
cord-009318-zt1o1bcz	513	40	and	and	CC
cord-009318-zt1o1bcz	513	41	delay	delay	VB
cord-009318-zt1o1bcz	513	42	the	the	DT
cord-009318-zt1o1bcz	513	43	start	start	NN
cord-009318-zt1o1bcz	513	44	of	of	IN
cord-009318-zt1o1bcz	513	45	amplification	amplification	NN
cord-009318-zt1o1bcz	513	46	at	at	IN
cord-009318-zt1o1bcz	513	47	clinically	clinically	RB
cord-009318-zt1o1bcz	513	48	relevant	relevant	JJ
cord-009318-zt1o1bcz	513	49	concentrations	concentration	NNS
cord-009318-zt1o1bcz	513	50	of	of	IN
cord-009318-zt1o1bcz	513	51	hgDNA	hgdna	NN
cord-009318-zt1o1bcz	513	52	.	.	.
cord-009318-zt1o1bcz	514	1	Thus	thus	RB
cord-009318-zt1o1bcz	514	2	,	,	,
cord-009318-zt1o1bcz	514	3	we	-PRON-	PRP
cord-009318-zt1o1bcz	514	4	conclude	conclude	VBP
cord-009318-zt1o1bcz	514	5	background	background	NN
cord-009318-zt1o1bcz	514	6	hgDNA	hgdna	JJ
cord-009318-zt1o1bcz	514	7	impacts	impact	VBZ
cord-009318-zt1o1bcz	514	8	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	514	9	for	for	IN
cord-009318-zt1o1bcz	514	10	this	this	DT
cord-009318-zt1o1bcz	514	11	primer	primer	NN
cord-009318-zt1o1bcz	514	12	set	set	NN
cord-009318-zt1o1bcz	514	13	.	.	.
cord-009318-zt1o1bcz	515	1	Generally	generally	RB
cord-009318-zt1o1bcz	515	2	,	,	,
cord-009318-zt1o1bcz	515	3	investigators	investigator	NNS
cord-009318-zt1o1bcz	515	4	should	should	MD
cord-009318-zt1o1bcz	515	5	examine	examine	VB
cord-009318-zt1o1bcz	515	6	their	-PRON-	PRP$
cord-009318-zt1o1bcz	515	7	own	own	JJ
cord-009318-zt1o1bcz	515	8	primer	primer	NN
cord-009318-zt1o1bcz	515	9	sets	set	NNS
cord-009318-zt1o1bcz	515	10	in	in	IN
cord-009318-zt1o1bcz	515	11	the	the	DT
cord-009318-zt1o1bcz	515	12	presence	presence	NN
cord-009318-zt1o1bcz	515	13	of	of	IN
cord-009318-zt1o1bcz	515	14	high	high	JJ
cord-009318-zt1o1bcz	515	15	concentrations	concentration	NNS
cord-009318-zt1o1bcz	515	16	of	of	IN
cord-009318-zt1o1bcz	515	17	hgDNA	hgdna	NN
cord-009318-zt1o1bcz	515	18	and	and	CC
cord-009318-zt1o1bcz	515	19	take	take	VB
cord-009318-zt1o1bcz	515	20	caution	caution	NN
cord-009318-zt1o1bcz	515	21	when	when	WRB
cord-009318-zt1o1bcz	515	22	examining	examine	VBG
cord-009318-zt1o1bcz	515	23	clinical	clinical	JJ
cord-009318-zt1o1bcz	515	24	samples	sample	NNS
cord-009318-zt1o1bcz	515	25	with	with	IN
cord-009318-zt1o1bcz	515	26	high	high	JJ
cord-009318-zt1o1bcz	515	27	leukocyte	leukocyte	NN
cord-009318-zt1o1bcz	515	28	concentrations	concentration	NNS
cord-009318-zt1o1bcz	515	29	(	(	-LRB-
cord-009318-zt1o1bcz	515	30	as	as	IN
cord-009318-zt1o1bcz	515	31	reported	report	VBN
cord-009318-zt1o1bcz	515	32	by	by	IN
cord-009318-zt1o1bcz	515	33	urinanalysis	urinanalysis	NN
cord-009318-zt1o1bcz	515	34	)	)	-RRB-
cord-009318-zt1o1bcz	515	35	.	.	.
cord-009318-zt1o1bcz	516	1	For	for	IN
cord-009318-zt1o1bcz	516	2	example	example	NN
cord-009318-zt1o1bcz	516	3	,	,	,
cord-009318-zt1o1bcz	516	4	CT	CT	NNP
cord-009318-zt1o1bcz	516	5	infection	infection	NN
cord-009318-zt1o1bcz	516	6	is	be	VBZ
cord-009318-zt1o1bcz	516	7	not	not	RB
cord-009318-zt1o1bcz	516	8	inherently	inherently	RB
cord-009318-zt1o1bcz	516	9	associated	associate	VBN
cord-009318-zt1o1bcz	516	10	with	with	IN
cord-009318-zt1o1bcz	516	11	high	high	JJ
cord-009318-zt1o1bcz	516	12	concentrations	concentration	NNS
cord-009318-zt1o1bcz	516	13	of	of	IN
cord-009318-zt1o1bcz	516	14	leukocytes	leukocyte	NNS
cord-009318-zt1o1bcz	516	15	and	and	CC
cord-009318-zt1o1bcz	516	16	many	many	JJ
cord-009318-zt1o1bcz	516	17	infections	infection	NNS
cord-009318-zt1o1bcz	516	18	are	be	VBP
cord-009318-zt1o1bcz	516	19	asymptomatic	asymptomatic	JJ
cord-009318-zt1o1bcz	516	20	.	.	.
cord-009318-zt1o1bcz	517	1	Ultimately	ultimately	RB
cord-009318-zt1o1bcz	517	2	,	,	,
cord-009318-zt1o1bcz	517	3	these	these	DT
cord-009318-zt1o1bcz	517	4	experiments	experiment	NNS
cord-009318-zt1o1bcz	517	5	underscore	underscore	VBP
cord-009318-zt1o1bcz	517	6	the	the	DT
cord-009318-zt1o1bcz	517	7	value	value	NN
cord-009318-zt1o1bcz	517	8	of	of	IN
cord-009318-zt1o1bcz	517	9	quantifying	quantify	VBG
cord-009318-zt1o1bcz	517	10	non	non	JJ
cord-009318-zt1o1bcz	517	11	-	-	JJ
cord-009318-zt1o1bcz	517	12	specific	specific	JJ
cord-009318-zt1o1bcz	517	13	amplificaiton	amplificaiton	JJ
cord-009318-zt1o1bcz	517	14	variability	variability	NN
cord-009318-zt1o1bcz	517	15	,	,	,
cord-009318-zt1o1bcz	517	16	using	use	VBG
cord-009318-zt1o1bcz	517	17	HRM	HRM	NNP
cord-009318-zt1o1bcz	517	18	,	,	,
cord-009318-zt1o1bcz	517	19	from	from	IN
cord-009318-zt1o1bcz	517	20	within	within	IN
cord-009318-zt1o1bcz	517	21	the	the	DT
cord-009318-zt1o1bcz	517	22	same	same	JJ
cord-009318-zt1o1bcz	517	23	experiment	experiment	NN
cord-009318-zt1o1bcz	517	24	as	as	IN
cord-009318-zt1o1bcz	517	25	a	a	DT
cord-009318-zt1o1bcz	517	26	target	target	NN
cord-009318-zt1o1bcz	517	27	is	be	VBZ
cord-009318-zt1o1bcz	517	28	quantified	quantify	VBN
cord-009318-zt1o1bcz	517	29	.	.	.
cord-009318-zt1o1bcz	518	1	Because	because	IN
cord-009318-zt1o1bcz	518	2	non	non	JJ
cord-009318-zt1o1bcz	518	3	-	-	JJ
cord-009318-zt1o1bcz	518	4	specific	specific	JJ
cord-009318-zt1o1bcz	518	5	amplificaiton	amplificaiton	NN
cord-009318-zt1o1bcz	518	6	is	be	VBZ
cord-009318-zt1o1bcz	518	7	measured	measure	VBN
cord-009318-zt1o1bcz	518	8	within	within	IN
cord-009318-zt1o1bcz	518	9	a	a	DT
cord-009318-zt1o1bcz	518	10	given	give	VBN
cord-009318-zt1o1bcz	518	11	sample	sample	NN
cord-009318-zt1o1bcz	518	12	,	,	,
cord-009318-zt1o1bcz	518	13	one	one	CD
cord-009318-zt1o1bcz	518	14	no	no	RB
cord-009318-zt1o1bcz	518	15	longer	longer	RB
cord-009318-zt1o1bcz	518	16	needs	need	VBZ
cord-009318-zt1o1bcz	518	17	to	to	TO
cord-009318-zt1o1bcz	518	18	assume	assume	VB
cord-009318-zt1o1bcz	518	19	it	-PRON-	PRP
cord-009318-zt1o1bcz	518	20	remains	remain	VBZ
cord-009318-zt1o1bcz	518	21	identical	identical	JJ
cord-009318-zt1o1bcz	518	22	to	to	IN
cord-009318-zt1o1bcz	518	23	the	the	DT
cord-009318-zt1o1bcz	518	24	NTC	NTC	NNP
cord-009318-zt1o1bcz	518	25	or	or	CC
cord-009318-zt1o1bcz	518	26	between	between	IN
cord-009318-zt1o1bcz	518	27	experimental	experimental	JJ
cord-009318-zt1o1bcz	518	28	runs	run	NNS
cord-009318-zt1o1bcz	518	29	.	.	.
cord-009318-zt1o1bcz	519	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	519	2	predict	predict	VBP
cord-009318-zt1o1bcz	519	3	that	that	IN
cord-009318-zt1o1bcz	519	4	the	the	DT
cord-009318-zt1o1bcz	519	5	combination	combination	NN
cord-009318-zt1o1bcz	519	6	of	of	IN
cord-009318-zt1o1bcz	519	7	HRM	HRM	NNP
cord-009318-zt1o1bcz	519	8	and	and	CC
cord-009318-zt1o1bcz	519	9	real	real	JJ
cord-009318-zt1o1bcz	519	10	-	-	HYPH
cord-009318-zt1o1bcz	519	11	time	time	NN
cord-009318-zt1o1bcz	519	12	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	519	13	will	will	MD
cord-009318-zt1o1bcz	519	14	be	be	VB
cord-009318-zt1o1bcz	519	15	invaluable	invaluable	JJ
cord-009318-zt1o1bcz	519	16	for	for	IN
cord-009318-zt1o1bcz	519	17	answering	answer	VBG
cord-009318-zt1o1bcz	519	18	many	many	JJ
cord-009318-zt1o1bcz	519	19	questions	question	NNS
cord-009318-zt1o1bcz	519	20	across	across	IN
cord-009318-zt1o1bcz	519	21	a	a	DT
cord-009318-zt1o1bcz	519	22	wide	wide	JJ
cord-009318-zt1o1bcz	519	23	variety	variety	NN
cord-009318-zt1o1bcz	519	24	of	of	IN
cord-009318-zt1o1bcz	519	25	applications	application	NNS
cord-009318-zt1o1bcz	519	26	,	,	,
cord-009318-zt1o1bcz	519	27	and	and	CC
cord-009318-zt1o1bcz	519	28	thus	thus	RB
cord-009318-zt1o1bcz	519	29	our	-PRON-	PRP$
cord-009318-zt1o1bcz	519	30	approach	approach	NN
cord-009318-zt1o1bcz	519	31	was	be	VBD
cord-009318-zt1o1bcz	519	32	designed	design	VBN
cord-009318-zt1o1bcz	519	33	to	to	TO
cord-009318-zt1o1bcz	519	34	be	be	VB
cord-009318-zt1o1bcz	519	35	accessible	accessible	JJ
cord-009318-zt1o1bcz	519	36	to	to	IN
cord-009318-zt1o1bcz	519	37	most	most	JJS
cord-009318-zt1o1bcz	519	38	standard	standard	JJ
cord-009318-zt1o1bcz	519	39	labs	lab	NNS
cord-009318-zt1o1bcz	519	40	.	.	.
cord-009318-zt1o1bcz	520	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	520	2	employed	employ	VBD
cord-009318-zt1o1bcz	520	3	commercial	commercial	JJ
cord-009318-zt1o1bcz	520	4	chips	chip	NNS
cord-009318-zt1o1bcz	520	5	for	for	IN
cord-009318-zt1o1bcz	520	6	digitization	digitization	NN
cord-009318-zt1o1bcz	520	7	,	,	,
cord-009318-zt1o1bcz	520	8	a	a	DT
cord-009318-zt1o1bcz	520	9	commercial	commercial	JJ
cord-009318-zt1o1bcz	520	10	thermoelectric	thermoelectric	JJ
cord-009318-zt1o1bcz	520	11	unit	unit	NN
cord-009318-zt1o1bcz	520	12	for	for	IN
cord-009318-zt1o1bcz	520	13	heating	heating	NN
cord-009318-zt1o1bcz	520	14	and	and	CC
cord-009318-zt1o1bcz	520	15	cooling	cooling	NN
cord-009318-zt1o1bcz	520	16	,	,	,
cord-009318-zt1o1bcz	520	17	a	a	DT
cord-009318-zt1o1bcz	520	18	commercial	commercial	JJ
cord-009318-zt1o1bcz	520	19	microscope	microscope	NN
cord-009318-zt1o1bcz	520	20	for	for	IN
cord-009318-zt1o1bcz	520	21	optical	optical	JJ
cord-009318-zt1o1bcz	520	22	analyses	analysis	NNS
cord-009318-zt1o1bcz	520	23	and	and	CC
cord-009318-zt1o1bcz	520	24	we	-PRON-	PRP
cord-009318-zt1o1bcz	520	25	made	make	VBD
cord-009318-zt1o1bcz	520	26	our	-PRON-	PRP$
cord-009318-zt1o1bcz	520	27	dataprocessing	dataprocessing	NN
cord-009318-zt1o1bcz	520	28	script	script	NN
cord-009318-zt1o1bcz	520	29	freely	freely	RB
cord-009318-zt1o1bcz	520	30	available	available	JJ
cord-009318-zt1o1bcz	520	31	.	.	.
cord-009318-zt1o1bcz	521	1	Our	-PRON-	PRP$
cord-009318-zt1o1bcz	521	2	intention	intention	NN
cord-009318-zt1o1bcz	521	3	was	be	VBD
cord-009318-zt1o1bcz	521	4	to	to	TO
cord-009318-zt1o1bcz	521	5	design	design	VB
cord-009318-zt1o1bcz	521	6	an	an	DT
cord-009318-zt1o1bcz	521	7	accessible	accessible	JJ
cord-009318-zt1o1bcz	521	8	system	system	NN
cord-009318-zt1o1bcz	521	9	with	with	IN
cord-009318-zt1o1bcz	521	10	readily	readily	RB
cord-009318-zt1o1bcz	521	11	available	available	JJ
cord-009318-zt1o1bcz	521	12	components	component	NNS
cord-009318-zt1o1bcz	521	13	to	to	TO
cord-009318-zt1o1bcz	521	14	enable	enable	VB
cord-009318-zt1o1bcz	521	15	others	other	NNS
cord-009318-zt1o1bcz	521	16	to	to	TO
cord-009318-zt1o1bcz	521	17	access	access	VB
cord-009318-zt1o1bcz	521	18	the	the	DT
cord-009318-zt1o1bcz	521	19	advantages	advantage	NNS
cord-009318-zt1o1bcz	521	20	of	of	IN
cord-009318-zt1o1bcz	521	21	digital	digital	JJ
cord-009318-zt1o1bcz	521	22	microfluidics	microfluidic	NNS
cord-009318-zt1o1bcz	521	23	to	to	TO
cord-009318-zt1o1bcz	521	24	study	study	VB
cord-009318-zt1o1bcz	521	25	and	and	CC
cord-009318-zt1o1bcz	521	26	optimize	optimize	VB
cord-009318-zt1o1bcz	521	27	primer	primer	NN
cord-009318-zt1o1bcz	521	28	sets	set	NNS
cord-009318-zt1o1bcz	521	29	,	,	,
cord-009318-zt1o1bcz	521	30	enzymes	enzyme	NNS
cord-009318-zt1o1bcz	521	31	,	,	,
cord-009318-zt1o1bcz	521	32	and	and	CC
cord-009318-zt1o1bcz	521	33	reaction	reaction	NN
cord-009318-zt1o1bcz	521	34	conditions	condition	NNS
cord-009318-zt1o1bcz	521	35	of	of	IN
cord-009318-zt1o1bcz	521	36	interest	interest	NN
cord-009318-zt1o1bcz	521	37	to	to	IN
cord-009318-zt1o1bcz	521	38	them	-PRON-	PRP
cord-009318-zt1o1bcz	521	39	.	.	.
cord-009318-zt1o1bcz	522	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	522	2	predict	predict	VBP
cord-009318-zt1o1bcz	522	3	these	these	DT
cord-009318-zt1o1bcz	522	4	capabilities	capability	NNS
cord-009318-zt1o1bcz	522	5	will	will	MD
cord-009318-zt1o1bcz	522	6	be	be	VB
cord-009318-zt1o1bcz	522	7	particularly	particularly	RB
cord-009318-zt1o1bcz	522	8	valuable	valuable	JJ
cord-009318-zt1o1bcz	522	9	for	for	IN
cord-009318-zt1o1bcz	522	10	people	people	NNS
cord-009318-zt1o1bcz	522	11	working	work	VBG
cord-009318-zt1o1bcz	522	12	with	with	IN
cord-009318-zt1o1bcz	522	13	variable	variable	JJ
cord-009318-zt1o1bcz	522	14	sample	sample	NN
cord-009318-zt1o1bcz	522	15	matrixes	matrix	NNS
cord-009318-zt1o1bcz	522	16	,	,	,
cord-009318-zt1o1bcz	522	17	high	high	JJ
cord-009318-zt1o1bcz	522	18	background	background	NN
cord-009318-zt1o1bcz	522	19	DNA	dna	NN
cord-009318-zt1o1bcz	522	20	,	,	,
cord-009318-zt1o1bcz	522	21	poorly	poorly	RB
cord-009318-zt1o1bcz	522	22	performing	perform	VBG
cord-009318-zt1o1bcz	522	23	primer	primer	NN
cord-009318-zt1o1bcz	522	24	sets	set	NNS
cord-009318-zt1o1bcz	522	25	,	,	,
cord-009318-zt1o1bcz	522	26	or	or	CC
cord-009318-zt1o1bcz	522	27	poorly	poorly	RB
cord-009318-zt1o1bcz	522	28	performing	perform	VBG
cord-009318-zt1o1bcz	522	29	enzymes	enzyme	NNS
cord-009318-zt1o1bcz	522	30	.	.	.
cord-009318-zt1o1bcz	523	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	523	2	derived	derive	VBD
cord-009318-zt1o1bcz	523	3	four	four	CD
cord-009318-zt1o1bcz	523	4	major	major	JJ
cord-009318-zt1o1bcz	523	5	lessons	lesson	NNS
cord-009318-zt1o1bcz	523	6	from	from	IN
cord-009318-zt1o1bcz	523	7	this	this	DT
cord-009318-zt1o1bcz	523	8	study	study	NN
cord-009318-zt1o1bcz	523	9	.	.	.
cord-009318-zt1o1bcz	524	1	First	first	RB
cord-009318-zt1o1bcz	524	2	,	,	,
cord-009318-zt1o1bcz	524	3	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	524	4	can	can	MD
cord-009318-zt1o1bcz	524	5	produce	produce	VB
cord-009318-zt1o1bcz	524	6	non	non	JJ
cord-009318-zt1o1bcz	524	7	-	-	JJ
cord-009318-zt1o1bcz	524	8	specific	specific	JJ
cord-009318-zt1o1bcz	524	9	amplicons	amplicon	NNS
cord-009318-zt1o1bcz	524	10	with	with	IN
cord-009318-zt1o1bcz	524	11	high	high	JJ
cord-009318-zt1o1bcz	524	12	T	t	NN
cord-009318-zt1o1bcz	524	13	m	m	NN
cord-009318-zt1o1bcz	524	14	.	.	.
cord-009318-zt1o1bcz	525	1	The	the	DT
cord-009318-zt1o1bcz	525	2	formation	formation	NN
cord-009318-zt1o1bcz	525	3	of	of	IN
cord-009318-zt1o1bcz	525	4	these	these	DT
cord-009318-zt1o1bcz	525	5	non	non	JJ
cord-009318-zt1o1bcz	525	6	-	-	JJ
cord-009318-zt1o1bcz	525	7	specific	specific	JJ
cord-009318-zt1o1bcz	525	8	amplicons	amplicon	NNS
cord-009318-zt1o1bcz	525	9	occurs	occur	VBZ
cord-009318-zt1o1bcz	525	10	from	from	IN
cord-009318-zt1o1bcz	525	11	the	the	DT
cord-009318-zt1o1bcz	525	12	interaction	interaction	NN
cord-009318-zt1o1bcz	525	13	of	of	IN
cord-009318-zt1o1bcz	525	14	multiple	multiple	JJ
cord-009318-zt1o1bcz	525	15	primers	primer	NNS
cord-009318-zt1o1bcz	525	16	and	and	CC
cord-009318-zt1o1bcz	525	17	the	the	DT
cord-009318-zt1o1bcz	525	18	use	use	NN
cord-009318-zt1o1bcz	525	19	of	of	IN
cord-009318-zt1o1bcz	525	20	a	a	DT
cord-009318-zt1o1bcz	525	21	polymerase	polymerase	NN
cord-009318-zt1o1bcz	525	22	with	with	IN
cord-009318-zt1o1bcz	525	23	template	template	NN
cord-009318-zt1o1bcz	525	24	switching	switching	NN
cord-009318-zt1o1bcz	525	25	ability	ability	NN
cord-009318-zt1o1bcz	525	26	,	,	,
cord-009318-zt1o1bcz	525	27	terminal	terminal	JJ
cord-009318-zt1o1bcz	525	28	transferase	transferase	NN
cord-009318-zt1o1bcz	525	29	activity	activity	NN
cord-009318-zt1o1bcz	525	30	and	and	CC
cord-009318-zt1o1bcz	525	31	lacking	lack	VBG
cord-009318-zt1o1bcz	525	32	3	3	CD
cord-009318-zt1o1bcz	525	33	-5	-5	CD
cord-009318-zt1o1bcz	525	34	exonuclease	exonuclease	NN
cord-009318-zt1o1bcz	525	35	activity	activity	NN
cord-009318-zt1o1bcz	525	36	.	.	.
cord-009318-zt1o1bcz	526	1	Interaction	interaction	NN
cord-009318-zt1o1bcz	526	2	of	of	IN
cord-009318-zt1o1bcz	526	3	primers	primer	NNS
cord-009318-zt1o1bcz	526	4	may	may	MD
cord-009318-zt1o1bcz	526	5	lead	lead	VB
cord-009318-zt1o1bcz	526	6	not	not	RB
cord-009318-zt1o1bcz	526	7	only	only	RB
cord-009318-zt1o1bcz	526	8	lead	lead	VB
cord-009318-zt1o1bcz	526	9	to	to	IN
cord-009318-zt1o1bcz	526	10	rising	rise	VBG
cord-009318-zt1o1bcz	526	11	background	background	NN
cord-009318-zt1o1bcz	526	12	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	526	13	(	(	-LRB-
cord-009318-zt1o1bcz	526	14	37	37	CD
cord-009318-zt1o1bcz	526	15	)	)	-RRB-
cord-009318-zt1o1bcz	526	16	,	,	,
cord-009318-zt1o1bcz	526	17	but	but	CC
cord-009318-zt1o1bcz	526	18	to	to	IN
cord-009318-zt1o1bcz	526	19	spontaneous	spontaneous	JJ
cord-009318-zt1o1bcz	526	20	exponential	exponential	JJ
cord-009318-zt1o1bcz	526	21	amplification	amplification	NN
cord-009318-zt1o1bcz	526	22	as	as	RB
cord-009318-zt1o1bcz	526	23	well	well	RB
cord-009318-zt1o1bcz	526	24	.	.	.
cord-009318-zt1o1bcz	527	1	Primer	primer	NN
cord-009318-zt1o1bcz	527	2	design	design	NN
cord-009318-zt1o1bcz	527	3	and	and	CC
cord-009318-zt1o1bcz	527	4	enzyme	enzyme	NN
cord-009318-zt1o1bcz	527	5	selection	selection	NN
cord-009318-zt1o1bcz	527	6	therefore	therefore	RB
cord-009318-zt1o1bcz	527	7	should	should	MD
cord-009318-zt1o1bcz	527	8	be	be	VB
cord-009318-zt1o1bcz	527	9	judicious	judicious	JJ
cord-009318-zt1o1bcz	527	10	to	to	TO
cord-009318-zt1o1bcz	527	11	avoid	avoid	VB
cord-009318-zt1o1bcz	527	12	formation	formation	NN
cord-009318-zt1o1bcz	527	13	of	of	IN
cord-009318-zt1o1bcz	527	14	hairpins	hairpin	NNS
cord-009318-zt1o1bcz	527	15	within	within	IN
cord-009318-zt1o1bcz	527	16	primers	primer	NNS
cord-009318-zt1o1bcz	527	17	,	,	,
cord-009318-zt1o1bcz	527	18	as	as	RB
cord-009318-zt1o1bcz	527	19	well	well	RB
cord-009318-zt1o1bcz	527	20	as	as	IN
cord-009318-zt1o1bcz	527	21	microhomology	microhomology	NN
cord-009318-zt1o1bcz	527	22	at	at	IN
cord-009318-zt1o1bcz	527	23	the	the	DT
cord-009318-zt1o1bcz	527	24	3	3	CD
cord-009318-zt1o1bcz	527	25	with	with	IN
cord-009318-zt1o1bcz	527	26	any	any	DT
cord-009318-zt1o1bcz	527	27	other	other	JJ
cord-009318-zt1o1bcz	527	28	primer	primer	NN
cord-009318-zt1o1bcz	527	29	,	,	,
cord-009318-zt1o1bcz	527	30	in	in	IN
cord-009318-zt1o1bcz	527	31	order	order	NN
cord-009318-zt1o1bcz	527	32	to	to	TO
cord-009318-zt1o1bcz	527	33	prevent	prevent	VB
cord-009318-zt1o1bcz	527	34	non	non	JJ
cord-009318-zt1o1bcz	527	35	-	-	JJ
cord-009318-zt1o1bcz	527	36	specific	specific	JJ
cord-009318-zt1o1bcz	527	37	amplification	amplification	NN
cord-009318-zt1o1bcz	527	38	.	.	.
cord-009318-zt1o1bcz	528	1	Second	second	RB
cord-009318-zt1o1bcz	528	2	,	,	,
cord-009318-zt1o1bcz	528	3	HRM	HRM	NNP
cord-009318-zt1o1bcz	528	4	in	in	IN
cord-009318-zt1o1bcz	528	5	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	528	6	is	be	VBZ
cord-009318-zt1o1bcz	528	7	a	a	DT
cord-009318-zt1o1bcz	528	8	useful	useful	JJ
cord-009318-zt1o1bcz	528	9	method	method	NN
cord-009318-zt1o1bcz	528	10	for	for	IN
cord-009318-zt1o1bcz	528	11	differentiating	differentiate	VBG
cord-009318-zt1o1bcz	528	12	specific	specific	JJ
cord-009318-zt1o1bcz	528	13	and	and	CC
cord-009318-zt1o1bcz	528	14	non	non	JJ
cord-009318-zt1o1bcz	528	15	-	-	JJ
cord-009318-zt1o1bcz	528	16	specific	specific	JJ
cord-009318-zt1o1bcz	528	17	amplification	amplification	NN
cord-009318-zt1o1bcz	528	18	events	event	NNS
cord-009318-zt1o1bcz	528	19	.	.	.
cord-009318-zt1o1bcz	529	1	Digital	digital	JJ
cord-009318-zt1o1bcz	529	2	experiments	experiment	NNS
cord-009318-zt1o1bcz	529	3	measure	measure	VBP
cord-009318-zt1o1bcz	529	4	the	the	DT
cord-009318-zt1o1bcz	529	5	fate	fate	NN
cord-009318-zt1o1bcz	529	6	and	and	CC
cord-009318-zt1o1bcz	529	7	rate	rate	NN
cord-009318-zt1o1bcz	529	8	of	of	IN
cord-009318-zt1o1bcz	529	9	each	each	DT
cord-009318-zt1o1bcz	529	10	template	template	NN
cord-009318-zt1o1bcz	529	11	,	,	,
cord-009318-zt1o1bcz	529	12	in	in	IN
cord-009318-zt1o1bcz	529	13	contrast	contrast	NN
cord-009318-zt1o1bcz	529	14	,	,	,
cord-009318-zt1o1bcz	529	15	bulk	bulk	JJ
cord-009318-zt1o1bcz	529	16	experiments	experiment	NNS
cord-009318-zt1o1bcz	529	17	are	be	VBP
cord-009318-zt1o1bcz	529	18	biased	bias	VBN
cord-009318-zt1o1bcz	529	19	toward	toward	IN
cord-009318-zt1o1bcz	529	20	early	early	JJ
cord-009318-zt1o1bcz	529	21	amplification	amplification	NN
cord-009318-zt1o1bcz	529	22	events	event	NNS
cord-009318-zt1o1bcz	529	23	.	.	.
cord-009318-zt1o1bcz	530	1	The	the	DT
cord-009318-zt1o1bcz	530	2	combination	combination	NN
cord-009318-zt1o1bcz	530	3	of	of	IN
cord-009318-zt1o1bcz	530	4	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	530	5	and	and	CC
cord-009318-zt1o1bcz	530	6	HRM	HRM	NNP
cord-009318-zt1o1bcz	530	7	allows	allow	VBZ
cord-009318-zt1o1bcz	530	8	observation	observation	NN
cord-009318-zt1o1bcz	530	9	of	of	IN
cord-009318-zt1o1bcz	530	10	many	many	JJ
cord-009318-zt1o1bcz	530	11	amplification	amplification	NN
cord-009318-zt1o1bcz	530	12	events	event	NNS
cord-009318-zt1o1bcz	530	13	and	and	CC
cord-009318-zt1o1bcz	530	14	assignment	assignment	NN
cord-009318-zt1o1bcz	530	15	of	of	IN
cord-009318-zt1o1bcz	530	16	the	the	DT
cord-009318-zt1o1bcz	530	17	nature	nature	NN
cord-009318-zt1o1bcz	530	18	of	of	IN
cord-009318-zt1o1bcz	530	19	that	that	DT
cord-009318-zt1o1bcz	530	20	amplification	amplification	NN
cord-009318-zt1o1bcz	530	21	as	as	IN
cord-009318-zt1o1bcz	530	22	true	true	JJ
cord-009318-zt1o1bcz	530	23	or	or	CC
cord-009318-zt1o1bcz	530	24	false	false	JJ
cord-009318-zt1o1bcz	530	25	.	.	.
cord-009318-zt1o1bcz	531	1	Further	further	RB
cord-009318-zt1o1bcz	531	2	,	,	,
cord-009318-zt1o1bcz	531	3	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	531	4	with	with	IN
cord-009318-zt1o1bcz	531	5	HRM	HRM	NNP
cord-009318-zt1o1bcz	531	6	quantifies	quantify	VBZ
cord-009318-zt1o1bcz	531	7	non	non	JJ
cord-009318-zt1o1bcz	531	8	-	-	JJ
cord-009318-zt1o1bcz	531	9	specific	specific	JJ
cord-009318-zt1o1bcz	531	10	amplification	amplification	NN
cord-009318-zt1o1bcz	531	11	experimentally	experimentally	RB
cord-009318-zt1o1bcz	531	12	in	in	IN
cord-009318-zt1o1bcz	531	13	the	the	DT
cord-009318-zt1o1bcz	531	14	presence	presence	NN
cord-009318-zt1o1bcz	531	15	of	of	IN
cord-009318-zt1o1bcz	531	16	specific	specific	JJ
cord-009318-zt1o1bcz	531	17	amplification	amplification	NN
cord-009318-zt1o1bcz	531	18	,	,	,
cord-009318-zt1o1bcz	531	19	eliminating	eliminate	VBG
cord-009318-zt1o1bcz	531	20	the	the	DT
cord-009318-zt1o1bcz	531	21	assumption	assumption	NN
cord-009318-zt1o1bcz	531	22	that	that	IN
cord-009318-zt1o1bcz	531	23	incidence	incidence	NN
cord-009318-zt1o1bcz	531	24	of	of	IN
cord-009318-zt1o1bcz	531	25	false	false	JJ
cord-009318-zt1o1bcz	531	26	positives	positive	NNS
cord-009318-zt1o1bcz	531	27	in	in	IN
cord-009318-zt1o1bcz	531	28	the	the	DT
cord-009318-zt1o1bcz	531	29	presence	presence	NN
cord-009318-zt1o1bcz	531	30	of	of	IN
cord-009318-zt1o1bcz	531	31	template	template	NN
cord-009318-zt1o1bcz	531	32	remains	remain	VBZ
cord-009318-zt1o1bcz	531	33	identical	identical	JJ
cord-009318-zt1o1bcz	531	34	to	to	IN
cord-009318-zt1o1bcz	531	35	the	the	DT
cord-009318-zt1o1bcz	531	36	NTC	NTC	NNP
cord-009318-zt1o1bcz	531	37	or	or	CC
cord-009318-zt1o1bcz	531	38	between	between	IN
cord-009318-zt1o1bcz	531	39	experimental	experimental	JJ
cord-009318-zt1o1bcz	531	40	runs	run	NNS
cord-009318-zt1o1bcz	531	41	.	.	.
cord-009318-zt1o1bcz	532	1	Third	third	RB
cord-009318-zt1o1bcz	532	2	,	,	,
cord-009318-zt1o1bcz	532	3	by	by	IN
cord-009318-zt1o1bcz	532	4	differentiating	differentiate	VBG
cord-009318-zt1o1bcz	532	5	specific	specific	JJ
cord-009318-zt1o1bcz	532	6	and	and	CC
cord-009318-zt1o1bcz	532	7	non	non	JJ
cord-009318-zt1o1bcz	532	8	-	-	JJ
cord-009318-zt1o1bcz	532	9	specific	specific	JJ
cord-009318-zt1o1bcz	532	10	amplification	amplification	NN
cord-009318-zt1o1bcz	532	11	,	,	,
cord-009318-zt1o1bcz	532	12	HRM	HRM	NNP
cord-009318-zt1o1bcz	532	13	is	be	VBZ
cord-009318-zt1o1bcz	532	14	helpful	helpful	JJ
cord-009318-zt1o1bcz	532	15	in	in	IN
cord-009318-zt1o1bcz	532	16	determining	determine	VBG
cord-009318-zt1o1bcz	532	17	the	the	DT
cord-009318-zt1o1bcz	532	18	combination	combination	NN
cord-009318-zt1o1bcz	532	19	processing	processing	NN
cord-009318-zt1o1bcz	532	20	and	and	CC
cord-009318-zt1o1bcz	532	21	assay	assay	NN
cord-009318-zt1o1bcz	532	22	parameters	parameter	NNS
cord-009318-zt1o1bcz	532	23	that	that	WDT
cord-009318-zt1o1bcz	532	24	will	will	MD
cord-009318-zt1o1bcz	532	25	lead	lead	VB
cord-009318-zt1o1bcz	532	26	to	to	IN
cord-009318-zt1o1bcz	532	27	the	the	DT
cord-009318-zt1o1bcz	532	28	best	good	JJS
cord-009318-zt1o1bcz	532	29	LOD	lod	NN
cord-009318-zt1o1bcz	532	30	in	in	IN
cord-009318-zt1o1bcz	532	31	a	a	DT
cord-009318-zt1o1bcz	532	32	digital	digital	JJ
cord-009318-zt1o1bcz	532	33	assay	assay	NN
cord-009318-zt1o1bcz	532	34	.	.	.
cord-009318-zt1o1bcz	533	1	When	when	WRB
cord-009318-zt1o1bcz	533	2	HRM	HRM	NNP
cord-009318-zt1o1bcz	533	3	is	be	VBZ
cord-009318-zt1o1bcz	533	4	incorporated	incorporate	VBN
cord-009318-zt1o1bcz	533	5	into	into	IN
cord-009318-zt1o1bcz	533	6	a	a	DT
cord-009318-zt1o1bcz	533	7	dLAMP	dlamp	NN
cord-009318-zt1o1bcz	533	8	assay	assay	NN
cord-009318-zt1o1bcz	533	9	,	,	,
cord-009318-zt1o1bcz	533	10	true	true	JJ
cord-009318-zt1o1bcz	533	11	and	and	CC
cord-009318-zt1o1bcz	533	12	false	false	JJ
cord-009318-zt1o1bcz	533	13	-	-	HYPH
cord-009318-zt1o1bcz	533	14	positive	positive	JJ
cord-009318-zt1o1bcz	533	15	amplification	amplification	NN
cord-009318-zt1o1bcz	533	16	events	event	NNS
cord-009318-zt1o1bcz	533	17	can	can	MD
cord-009318-zt1o1bcz	533	18	easily	easily	RB
cord-009318-zt1o1bcz	533	19	be	be	VB
cord-009318-zt1o1bcz	533	20	separated	separate	VBN
cord-009318-zt1o1bcz	533	21	.	.	.
cord-009318-zt1o1bcz	534	1	LOD	LOD	NNP
cord-009318-zt1o1bcz	534	2	is	be	VBZ
cord-009318-zt1o1bcz	534	3	improved	improve	VBN
cord-009318-zt1o1bcz	534	4	by	by	IN
cord-009318-zt1o1bcz	534	5	elimination	elimination	NN
cord-009318-zt1o1bcz	534	6	of	of	IN
cord-009318-zt1o1bcz	534	7	non	non	JJ
cord-009318-zt1o1bcz	534	8	-	-	JJ
cord-009318-zt1o1bcz	534	9	specific	specific	JJ
cord-009318-zt1o1bcz	534	10	background	background	NN
cord-009318-zt1o1bcz	534	11	and	and	CC
cord-009318-zt1o1bcz	534	12	thus	thus	RB
cord-009318-zt1o1bcz	534	13	becomes	become	VBZ
cord-009318-zt1o1bcz	534	14	dependent	dependent	JJ
cord-009318-zt1o1bcz	534	15	on	on	IN
cord-009318-zt1o1bcz	534	16	the	the	DT
cord-009318-zt1o1bcz	534	17	number	number	NN
cord-009318-zt1o1bcz	534	18	of	of	IN
cord-009318-zt1o1bcz	534	19	molecules	molecule	NNS
cord-009318-zt1o1bcz	534	20	that	that	WDT
cord-009318-zt1o1bcz	534	21	amplify	amplify	VBP
cord-009318-zt1o1bcz	534	22	(	(	-LRB-
cord-009318-zt1o1bcz	534	23	i.e.	i.e.	FW
cord-009318-zt1o1bcz	534	24	amplification	amplification	NN
cord-009318-zt1o1bcz	534	25	efficiency	efficiency	NN
cord-009318-zt1o1bcz	534	26	or	or	CC
cord-009318-zt1o1bcz	534	27	fraction	fraction	NN
cord-009318-zt1o1bcz	534	28	of	of	IN
cord-009318-zt1o1bcz	534	29	copies	copy	NNS
cord-009318-zt1o1bcz	534	30	detected	detect	VBN
cord-009318-zt1o1bcz	534	31	)	)	-RRB-
cord-009318-zt1o1bcz	534	32	,	,	,
cord-009318-zt1o1bcz	534	33	without	without	IN
cord-009318-zt1o1bcz	534	34	dependence	dependence	NN
cord-009318-zt1o1bcz	534	35	on	on	IN
cord-009318-zt1o1bcz	534	36	the	the	DT
cord-009318-zt1o1bcz	534	37	incidence	incidence	NN
cord-009318-zt1o1bcz	534	38	of	of	IN
cord-009318-zt1o1bcz	534	39	false	false	JJ
cord-009318-zt1o1bcz	534	40	positives	positive	NNS
cord-009318-zt1o1bcz	534	41	.	.	.
cord-009318-zt1o1bcz	535	1	In	in	IN
cord-009318-zt1o1bcz	535	2	contrast	contrast	NN
cord-009318-zt1o1bcz	535	3	,	,	,
cord-009318-zt1o1bcz	535	4	if	if	IN
cord-009318-zt1o1bcz	535	5	HRM	HRM	NNP
cord-009318-zt1o1bcz	535	6	were	be	VBD
cord-009318-zt1o1bcz	535	7	employed	employ	VBN
cord-009318-zt1o1bcz	535	8	in	in	IN
cord-009318-zt1o1bcz	535	9	a	a	DT
cord-009318-zt1o1bcz	535	10	bulk	bulk	JJ
cord-009318-zt1o1bcz	535	11	reaction	reaction	NN
cord-009318-zt1o1bcz	535	12	,	,	,
cord-009318-zt1o1bcz	535	13	the	the	DT
cord-009318-zt1o1bcz	535	14	LOD	lod	NN
cord-009318-zt1o1bcz	535	15	would	would	MD
cord-009318-zt1o1bcz	535	16	still	still	RB
cord-009318-zt1o1bcz	535	17	be	be	VB
cord-009318-zt1o1bcz	535	18	limited	limit	VBN
cord-009318-zt1o1bcz	535	19	by	by	IN
cord-009318-zt1o1bcz	535	20	the	the	DT
cord-009318-zt1o1bcz	535	21	competition	competition	NN
cord-009318-zt1o1bcz	535	22	between	between	IN
cord-009318-zt1o1bcz	535	23	specific	specific	JJ
cord-009318-zt1o1bcz	535	24	and	and	CC
cord-009318-zt1o1bcz	535	25	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	535	26	amplification	amplification	NN
cord-009318-zt1o1bcz	535	27	(	(	-LRB-
cord-009318-zt1o1bcz	535	28	which	which	WDT
cord-009318-zt1o1bcz	535	29	amplifies	amplify	VBZ
cord-009318-zt1o1bcz	535	30	first	first	JJ
cord-009318-zt1o1bcz	535	31	)	)	-RRB-
cord-009318-zt1o1bcz	535	32	and	and	CC
cord-009318-zt1o1bcz	535	33	would	would	MD
cord-009318-zt1o1bcz	535	34	require	require	VB
cord-009318-zt1o1bcz	535	35	a	a	DT
cord-009318-zt1o1bcz	535	36	high	high	JJ
cord-009318-zt1o1bcz	535	37	number	number	NN
cord-009318-zt1o1bcz	535	38	of	of	IN
cord-009318-zt1o1bcz	535	39	trials	trial	NNS
cord-009318-zt1o1bcz	535	40	to	to	TO
cord-009318-zt1o1bcz	535	41	achieve	achieve	VB
cord-009318-zt1o1bcz	535	42	sufficient	sufficient	JJ
cord-009318-zt1o1bcz	535	43	statistical	statistical	JJ
cord-009318-zt1o1bcz	535	44	power	power	NN
cord-009318-zt1o1bcz	535	45	.	.	.
cord-009318-zt1o1bcz	536	1	Importantly	importantly	RB
cord-009318-zt1o1bcz	536	2	,	,	,
cord-009318-zt1o1bcz	536	3	even	even	RB
cord-009318-zt1o1bcz	536	4	when	when	WRB
cord-009318-zt1o1bcz	536	5	HRM	HRM	NNP
cord-009318-zt1o1bcz	536	6	will	will	MD
cord-009318-zt1o1bcz	536	7	not	not	RB
cord-009318-zt1o1bcz	536	8	be	be	VB
cord-009318-zt1o1bcz	536	9	used	use	VBN
cord-009318-zt1o1bcz	536	10	in	in	IN
cord-009318-zt1o1bcz	536	11	the	the	DT
cord-009318-zt1o1bcz	536	12	final	final	JJ
cord-009318-zt1o1bcz	536	13	assay	assay	NN
cord-009318-zt1o1bcz	536	14	,	,	,
cord-009318-zt1o1bcz	536	15	it	-PRON-	PRP
cord-009318-zt1o1bcz	536	16	can	can	MD
cord-009318-zt1o1bcz	536	17	still	still	RB
cord-009318-zt1o1bcz	536	18	be	be	VB
cord-009318-zt1o1bcz	536	19	incorporated	incorporate	VBN
cord-009318-zt1o1bcz	536	20	during	during	IN
cord-009318-zt1o1bcz	536	21	the	the	DT
cord-009318-zt1o1bcz	536	22	assaydevelopment	assaydevelopment	NN
cord-009318-zt1o1bcz	536	23	stage	stage	NN
cord-009318-zt1o1bcz	536	24	to	to	TO
cord-009318-zt1o1bcz	536	25	improve	improve	VB
cord-009318-zt1o1bcz	536	26	the	the	DT
cord-009318-zt1o1bcz	536	27	assay	assay	NN
cord-009318-zt1o1bcz	536	28	's	's	POS
cord-009318-zt1o1bcz	536	29	LOD	lod	NN
cord-009318-zt1o1bcz	536	30	by	by	IN
cord-009318-zt1o1bcz	536	31	determin	determin	VB
cord-009318-zt1o1bcz	536	32	-	-	HYPH
cord-009318-zt1o1bcz	536	33	ing	e	VBG
cord-009318-zt1o1bcz	536	34	the	the	DT
cord-009318-zt1o1bcz	536	35	optimal	optimal	JJ
cord-009318-zt1o1bcz	536	36	choice	choice	NN
cord-009318-zt1o1bcz	536	37	of	of	IN
cord-009318-zt1o1bcz	536	38	parameters	parameter	NNS
cord-009318-zt1o1bcz	536	39	based	base	VBN
cord-009318-zt1o1bcz	536	40	on	on	IN
cord-009318-zt1o1bcz	536	41	rate	rate	NN
cord-009318-zt1o1bcz	536	42	,	,	,
cord-009318-zt1o1bcz	536	43	TTP	TTP	NNP
cord-009318-zt1o1bcz	536	44	,	,	,
cord-009318-zt1o1bcz	536	45	final	final	JJ
cord-009318-zt1o1bcz	536	46	intensity	intensity	NN
cord-009318-zt1o1bcz	536	47	or	or	CC
cord-009318-zt1o1bcz	536	48	any	any	DT
cord-009318-zt1o1bcz	536	49	combination	combination	NN
cord-009318-zt1o1bcz	536	50	of	of	IN
cord-009318-zt1o1bcz	536	51	these	these	DT
cord-009318-zt1o1bcz	536	52	parameters	parameter	NNS
cord-009318-zt1o1bcz	536	53	.	.	.
cord-009318-zt1o1bcz	537	1	Furthermore	furthermore	RB
cord-009318-zt1o1bcz	537	2	,	,	,
cord-009318-zt1o1bcz	537	3	our	-PRON-	PRP$
cord-009318-zt1o1bcz	537	4	mathematical	mathematical	JJ
cord-009318-zt1o1bcz	537	5	description	description	NN
cord-009318-zt1o1bcz	537	6	of	of	IN
cord-009318-zt1o1bcz	537	7	LOD	LOD	NNP
cord-009318-zt1o1bcz	537	8	is	be	VBZ
cord-009318-zt1o1bcz	537	9	generalizable	generalizable	JJ
cord-009318-zt1o1bcz	537	10	to	to	IN
cord-009318-zt1o1bcz	537	11	other	other	JJ
cord-009318-zt1o1bcz	537	12	amplification	amplification	NN
cord-009318-zt1o1bcz	537	13	methods	method	NNS
cord-009318-zt1o1bcz	537	14	that	that	WDT
cord-009318-zt1o1bcz	537	15	are	be	VBP
cord-009318-zt1o1bcz	537	16	measured	measure	VBN
cord-009318-zt1o1bcz	537	17	in	in	IN
cord-009318-zt1o1bcz	537	18	digital	digital	NN
cord-009318-zt1o1bcz	537	19	and	and	CC
cord-009318-zt1o1bcz	537	20	can	can	MD
cord-009318-zt1o1bcz	537	21	separate	separate	VB
cord-009318-zt1o1bcz	537	22	specific	specific	JJ
cord-009318-zt1o1bcz	537	23	and	and	CC
cord-009318-zt1o1bcz	537	24	non	non	JJ
cord-009318-zt1o1bcz	537	25	-	-	JJ
cord-009318-zt1o1bcz	537	26	specific	specific	JJ
cord-009318-zt1o1bcz	537	27	amplification	amplification	NN
cord-009318-zt1o1bcz	537	28	.	.	.
cord-009318-zt1o1bcz	538	1	Fourth	fourth	JJ
cord-009318-zt1o1bcz	538	2	,	,	,
cord-009318-zt1o1bcz	538	3	high	high	JJ
cord-009318-zt1o1bcz	538	4	levels	level	NNS
cord-009318-zt1o1bcz	538	5	of	of	IN
cord-009318-zt1o1bcz	538	6	non	non	JJ
cord-009318-zt1o1bcz	538	7	-	-	JJ
cord-009318-zt1o1bcz	538	8	specific	specific	JJ
cord-009318-zt1o1bcz	538	9	host	host	NN
cord-009318-zt1o1bcz	538	10	gDNA	gdna	NN
cord-009318-zt1o1bcz	538	11	suppress	suppress	VB
cord-009318-zt1o1bcz	538	12	analytical	analytical	JJ
cord-009318-zt1o1bcz	538	13	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	538	14	and	and	CC
cord-009318-zt1o1bcz	538	15	specificity	specificity	NN
cord-009318-zt1o1bcz	538	16	,	,	,
cord-009318-zt1o1bcz	538	17	reduce	reduce	VB
cord-009318-zt1o1bcz	538	18	amplification	amplification	NN
cord-009318-zt1o1bcz	538	19	velocity	velocity	NN
cord-009318-zt1o1bcz	538	20	,	,	,
cord-009318-zt1o1bcz	538	21	and	and	CC
cord-009318-zt1o1bcz	538	22	delay	delay	VB
cord-009318-zt1o1bcz	538	23	the	the	DT
cord-009318-zt1o1bcz	538	24	start	start	NN
cord-009318-zt1o1bcz	538	25	of	of	IN
cord-009318-zt1o1bcz	538	26	amplification	amplification	NN
cord-009318-zt1o1bcz	538	27	.	.	.
cord-009318-zt1o1bcz	539	1	However	however	RB
cord-009318-zt1o1bcz	539	2	,	,	,
cord-009318-zt1o1bcz	539	3	lowto	lowto	NN
cord-009318-zt1o1bcz	539	4	-	-	HYPH
cord-009318-zt1o1bcz	539	5	moderate	moderate	JJ
cord-009318-zt1o1bcz	539	6	levels	level	NNS
cord-009318-zt1o1bcz	539	7	of	of	IN
cord-009318-zt1o1bcz	539	8	non	non	JJ
cord-009318-zt1o1bcz	539	9	-	-	JJ
cord-009318-zt1o1bcz	539	10	specific	specific	JJ
cord-009318-zt1o1bcz	539	11	host	host	NN
cord-009318-zt1o1bcz	539	12	gDNA	gDNA	NNS
cord-009318-zt1o1bcz	539	13	do	do	VBP
cord-009318-zt1o1bcz	539	14	not	not	RB
cord-009318-zt1o1bcz	539	15	impact	impact	VB
cord-009318-zt1o1bcz	539	16	the	the	DT
cord-009318-zt1o1bcz	539	17	analytical	analytical	JJ
cord-009318-zt1o1bcz	539	18	specificity	specificity	NN
cord-009318-zt1o1bcz	539	19	or	or	CC
cord-009318-zt1o1bcz	539	20	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	539	21	of	of	IN
cord-009318-zt1o1bcz	539	22	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	539	23	.	.	.
cord-009318-zt1o1bcz	540	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	540	2	ran	run	VBD
cord-009318-zt1o1bcz	540	3	our	-PRON-	PRP$
cord-009318-zt1o1bcz	540	4	assays	assay	NNS
cord-009318-zt1o1bcz	540	5	through	through	IN
cord-009318-zt1o1bcz	540	6	clinically	clinically	RB
cord-009318-zt1o1bcz	540	7	relevant	relevant	JJ
cord-009318-zt1o1bcz	540	8	concentrations	concentration	NNS
cord-009318-zt1o1bcz	540	9	of	of	IN
cord-009318-zt1o1bcz	540	10	background	background	NN
cord-009318-zt1o1bcz	540	11	DNA	dna	NN
cord-009318-zt1o1bcz	540	12	and	and	CC
cord-009318-zt1o1bcz	540	13	did	do	VBD
cord-009318-zt1o1bcz	540	14	not	not	RB
cord-009318-zt1o1bcz	540	15	observe	observe	VB
cord-009318-zt1o1bcz	540	16	interference	interference	NN
cord-009318-zt1o1bcz	540	17	until	until	IN
cord-009318-zt1o1bcz	540	18	the	the	DT
cord-009318-zt1o1bcz	540	19	upper	upper	JJ
cord-009318-zt1o1bcz	540	20	range	range	NN
cord-009318-zt1o1bcz	540	21	of	of	IN
cord-009318-zt1o1bcz	540	22	concentrations	concentration	NNS
cord-009318-zt1o1bcz	540	23	expected	expect	VBN
cord-009318-zt1o1bcz	540	24	to	to	TO
cord-009318-zt1o1bcz	540	25	cause	cause	VB
cord-009318-zt1o1bcz	540	26	interference	interference	NN
cord-009318-zt1o1bcz	540	27	to	to	TO
cord-009318-zt1o1bcz	540	28	demonstrate	demonstrate	VB
cord-009318-zt1o1bcz	540	29	the	the	DT
cord-009318-zt1o1bcz	540	30	clinical	clinical	JJ
cord-009318-zt1o1bcz	540	31	utility	utility	NN
cord-009318-zt1o1bcz	540	32	of	of	IN
cord-009318-zt1o1bcz	540	33	real	real	JJ
cord-009318-zt1o1bcz	540	34	-	-	HYPH
cord-009318-zt1o1bcz	540	35	time	time	NN
cord-009318-zt1o1bcz	540	36	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	540	37	with	with	IN
cord-009318-zt1o1bcz	540	38	HRM	HRM	NNP
cord-009318-zt1o1bcz	540	39	.	.	.
cord-009318-zt1o1bcz	541	1	Real	real	JJ
cord-009318-zt1o1bcz	541	2	-	-	HYPH
cord-009318-zt1o1bcz	541	3	time	time	NN
cord-009318-zt1o1bcz	541	4	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	541	5	with	with	IN
cord-009318-zt1o1bcz	541	6	HRM	HRM	NNP
cord-009318-zt1o1bcz	541	7	will	will	MD
cord-009318-zt1o1bcz	541	8	enable	enable	VB
cord-009318-zt1o1bcz	541	9	the	the	DT
cord-009318-zt1o1bcz	541	10	mechanistic	mechanistic	JJ
cord-009318-zt1o1bcz	541	11	optimization	optimization	NN
cord-009318-zt1o1bcz	541	12	of	of	IN
cord-009318-zt1o1bcz	541	13	primers	primer	NNS
cord-009318-zt1o1bcz	541	14	and	and	CC
cord-009318-zt1o1bcz	541	15	myriad	myriad	JJ
cord-009318-zt1o1bcz	541	16	assay	assay	NN
cord-009318-zt1o1bcz	541	17	conditions	condition	NNS
cord-009318-zt1o1bcz	541	18	(	(	-LRB-
cord-009318-zt1o1bcz	541	19	such	such	JJ
cord-009318-zt1o1bcz	541	20	as	as	IN
cord-009318-zt1o1bcz	541	21	buffer	buffer	NN
cord-009318-zt1o1bcz	541	22	,	,	,
cord-009318-zt1o1bcz	541	23	Mg	Mg	NNP
cord-009318-zt1o1bcz	541	24	2	2	CD
cord-009318-zt1o1bcz	541	25	+	+	SYM
cord-009318-zt1o1bcz	541	26	and	and	CC
cord-009318-zt1o1bcz	541	27	reaction	reaction	NN
cord-009318-zt1o1bcz	541	28	temperature	temperature	NN
cord-009318-zt1o1bcz	541	29	)	)	-RRB-
cord-009318-zt1o1bcz	541	30	.	.	.
cord-009318-zt1o1bcz	542	1	Because	because	IN
cord-009318-zt1o1bcz	542	2	real	real	JJ
cord-009318-zt1o1bcz	542	3	-	-	HYPH
cord-009318-zt1o1bcz	542	4	time	time	NN
cord-009318-zt1o1bcz	542	5	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	542	6	with	with	IN
cord-009318-zt1o1bcz	542	7	HRM	HRM	NNP
cord-009318-zt1o1bcz	542	8	reveals	reveal	VBZ
cord-009318-zt1o1bcz	542	9	the	the	DT
cord-009318-zt1o1bcz	542	10	incidence	incidence	NN
cord-009318-zt1o1bcz	542	11	of	of	IN
cord-009318-zt1o1bcz	542	12	nonspecific	nonspecific	JJ
cord-009318-zt1o1bcz	542	13	amplification	amplification	NN
cord-009318-zt1o1bcz	542	14	products	product	NNS
cord-009318-zt1o1bcz	542	15	with	with	IN
cord-009318-zt1o1bcz	542	16	high	high	JJ
cord-009318-zt1o1bcz	542	17	and	and	CC
cord-009318-zt1o1bcz	542	18	low	low	JJ
cord-009318-zt1o1bcz	542	19	T	t	NN
cord-009318-zt1o1bcz	542	20	m	m	NN
cord-009318-zt1o1bcz	542	21	as	as	IN
cord-009318-zt1o1bcz	542	22	a	a	DT
cord-009318-zt1o1bcz	542	23	function	function	NN
cord-009318-zt1o1bcz	542	24	of	of	IN
cord-009318-zt1o1bcz	542	25	time	time	NN
cord-009318-zt1o1bcz	542	26	,	,	,
cord-009318-zt1o1bcz	542	27	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	542	28	with	with	IN
cord-009318-zt1o1bcz	542	29	HRM	HRM	NNP
cord-009318-zt1o1bcz	542	30	can	can	MD
cord-009318-zt1o1bcz	542	31	be	be	VB
cord-009318-zt1o1bcz	542	32	used	use	VBN
cord-009318-zt1o1bcz	542	33	to	to	TO
cord-009318-zt1o1bcz	542	34	investigate	investigate	VB
cord-009318-zt1o1bcz	542	35	approaches	approach	NNS
cord-009318-zt1o1bcz	542	36	that	that	WDT
cord-009318-zt1o1bcz	542	37	will	will	MD
cord-009318-zt1o1bcz	542	38	eliminate	eliminate	VB
cord-009318-zt1o1bcz	542	39	different	different	JJ
cord-009318-zt1o1bcz	542	40	non	non	JJ
cord-009318-zt1o1bcz	542	41	-	-	JJ
cord-009318-zt1o1bcz	542	42	specific	specific	JJ
cord-009318-zt1o1bcz	542	43	products	product	NNS
cord-009318-zt1o1bcz	542	44	.	.	.
cord-009318-zt1o1bcz	543	1	For	for	IN
cord-009318-zt1o1bcz	543	2	example	example	NN
cord-009318-zt1o1bcz	543	3	,	,	,
cord-009318-zt1o1bcz	543	4	fast	fast	JJ
cord-009318-zt1o1bcz	543	5	or	or	CC
cord-009318-zt1o1bcz	543	6	early	early	JJ
cord-009318-zt1o1bcz	543	7	non	non	JJ
cord-009318-zt1o1bcz	543	8	-	-	JJ
cord-009318-zt1o1bcz	543	9	specific	specific	JJ
cord-009318-zt1o1bcz	543	10	events	event	NNS
cord-009318-zt1o1bcz	543	11	in	in	IN
cord-009318-zt1o1bcz	543	12	digital	digital	NNP
cord-009318-zt1o1bcz	543	13	may	may	MD
cord-009318-zt1o1bcz	543	14	indicate	indicate	VB
cord-009318-zt1o1bcz	543	15	primers	primer	NNS
cord-009318-zt1o1bcz	543	16	or	or	CC
cord-009318-zt1o1bcz	543	17	conditions	condition	NNS
cord-009318-zt1o1bcz	543	18	that	that	WDT
cord-009318-zt1o1bcz	543	19	will	will	MD
cord-009318-zt1o1bcz	543	20	be	be	VB
cord-009318-zt1o1bcz	543	21	especially	especially	RB
cord-009318-zt1o1bcz	543	22	vulnerable	vulnerable	JJ
cord-009318-zt1o1bcz	543	23	to	to	IN
cord-009318-zt1o1bcz	543	24	failure	failure	NN
cord-009318-zt1o1bcz	543	25	in	in	IN
cord-009318-zt1o1bcz	543	26	a	a	DT
cord-009318-zt1o1bcz	543	27	bulk	bulk	JJ
cord-009318-zt1o1bcz	543	28	reaction	reaction	NN
cord-009318-zt1o1bcz	543	29	.	.	.
cord-009318-zt1o1bcz	544	1	Thus	thus	RB
cord-009318-zt1o1bcz	544	2	,	,	,
cord-009318-zt1o1bcz	544	3	realtime	realtime	NNP
cord-009318-zt1o1bcz	544	4	dLAMP	dLAMP	NNP
cord-009318-zt1o1bcz	544	5	with	with	IN
cord-009318-zt1o1bcz	544	6	HRM	HRM	NNP
cord-009318-zt1o1bcz	544	7	could	could	MD
cord-009318-zt1o1bcz	544	8	be	be	VB
cord-009318-zt1o1bcz	544	9	used	use	VBN
cord-009318-zt1o1bcz	544	10	to	to	TO
cord-009318-zt1o1bcz	544	11	design	design	VB
cord-009318-zt1o1bcz	544	12	primers	primer	NNS
cord-009318-zt1o1bcz	544	13	that	that	WDT
cord-009318-zt1o1bcz	544	14	will	will	MD
cord-009318-zt1o1bcz	544	15	suppress	suppress	VB
cord-009318-zt1o1bcz	544	16	non	non	JJ
cord-009318-zt1o1bcz	544	17	-	-	JJ
cord-009318-zt1o1bcz	544	18	specific	specific	JJ
cord-009318-zt1o1bcz	544	19	amplification	amplification	NN
cord-009318-zt1o1bcz	544	20	in	in	IN
cord-009318-zt1o1bcz	544	21	bulk	bulk	NN
cord-009318-zt1o1bcz	544	22	,	,	,
cord-009318-zt1o1bcz	544	23	by	by	IN
cord-009318-zt1o1bcz	544	24	generating	generate	VBG
cord-009318-zt1o1bcz	544	25	only	only	RB
cord-009318-zt1o1bcz	544	26	non	non	JJ
cord-009318-zt1o1bcz	544	27	-	-	JJ
cord-009318-zt1o1bcz	544	28	specific	specific	JJ
cord-009318-zt1o1bcz	544	29	amplicons	amplicon	NNS
cord-009318-zt1o1bcz	544	30	that	that	WDT
cord-009318-zt1o1bcz	544	31	occur	occur	VBP
cord-009318-zt1o1bcz	544	32	at	at	IN
cord-009318-zt1o1bcz	544	33	slow	slow	JJ
cord-009318-zt1o1bcz	544	34	rates	rate	NNS
cord-009318-zt1o1bcz	544	35	and	and	CC
cord-009318-zt1o1bcz	544	36	late	late	JJ
cord-009318-zt1o1bcz	544	37	TTP	TTP	NNP
cord-009318-zt1o1bcz	544	38	.	.	.
cord-009318-zt1o1bcz	545	1	Future	future	JJ
cord-009318-zt1o1bcz	545	2	efforts	effort	NNS
cord-009318-zt1o1bcz	545	3	should	should	MD
cord-009318-zt1o1bcz	545	4	investigate	investigate	VB
cord-009318-zt1o1bcz	545	5	the	the	DT
cord-009318-zt1o1bcz	545	6	combination	combination	NN
cord-009318-zt1o1bcz	545	7	of	of	IN
cord-009318-zt1o1bcz	545	8	realtime	realtime	NNP
cord-009318-zt1o1bcz	545	9	dLAMP	dLAMP	NNP
cord-009318-zt1o1bcz	546	1	(	(	-LRB-
cord-009318-zt1o1bcz	546	2	and	and	CC
cord-009318-zt1o1bcz	546	3	other	other	JJ
cord-009318-zt1o1bcz	546	4	digital	digital	JJ
cord-009318-zt1o1bcz	546	5	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	546	6	amplification	amplification	NN
cord-009318-zt1o1bcz	546	7	technologies	technology	NNS
cord-009318-zt1o1bcz	546	8	)	)	-RRB-
cord-009318-zt1o1bcz	546	9	and	and	CC
cord-009318-zt1o1bcz	546	10	HRM	HRM	NNP
cord-009318-zt1o1bcz	546	11	as	as	IN
cord-009318-zt1o1bcz	546	12	a	a	DT
cord-009318-zt1o1bcz	546	13	way	way	NN
cord-009318-zt1o1bcz	546	14	to	to	TO
cord-009318-zt1o1bcz	546	15	increase	increase	VB
cord-009318-zt1o1bcz	546	16	multiplexing	multiplexing	NN
cord-009318-zt1o1bcz	546	17	of	of	IN
cord-009318-zt1o1bcz	546	18	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	546	19	when	when	WRB
cord-009318-zt1o1bcz	546	20	using	use	VBG
cord-009318-zt1o1bcz	546	21	a	a	DT
cord-009318-zt1o1bcz	546	22	single	single	JJ
cord-009318-zt1o1bcz	546	23	reporter	reporter	NN
cord-009318-zt1o1bcz	546	24	.	.	.
cord-009318-zt1o1bcz	547	1	In	in	IN
cord-009318-zt1o1bcz	547	2	PCR	PCR	NNP
cord-009318-zt1o1bcz	547	3	,	,	,
cord-009318-zt1o1bcz	547	4	HRM	HRM	NNP
cord-009318-zt1o1bcz	547	5	has	have	VBZ
cord-009318-zt1o1bcz	547	6	been	be	VBN
cord-009318-zt1o1bcz	547	7	used	use	VBN
cord-009318-zt1o1bcz	547	8	to	to	TO
cord-009318-zt1o1bcz	547	9	differentiate	differentiate	VB
cord-009318-zt1o1bcz	547	10	among	among	IN
cord-009318-zt1o1bcz	547	11	multiple	multiple	JJ
cord-009318-zt1o1bcz	547	12	amplification	amplification	NN
cord-009318-zt1o1bcz	547	13	products	product	NNS
cord-009318-zt1o1bcz	547	14	by	by	IN
cord-009318-zt1o1bcz	547	15	measuring	measure	VBG
cord-009318-zt1o1bcz	547	16	differences	difference	NNS
cord-009318-zt1o1bcz	547	17	in	in	IN
cord-009318-zt1o1bcz	547	18	T	T	NNP
cord-009318-zt1o1bcz	547	19	m	m	NNP
cord-009318-zt1o1bcz	547	20	(	(	-LRB-
cord-009318-zt1o1bcz	547	21	42	42	CD
cord-009318-zt1o1bcz	547	22	)	)	-RRB-
cord-009318-zt1o1bcz	547	23	(	(	-LRB-
cord-009318-zt1o1bcz	547	24	43	43	CD
cord-009318-zt1o1bcz	547	25	)	)	-RRB-
cord-009318-zt1o1bcz	547	26	(	(	-LRB-
cord-009318-zt1o1bcz	547	27	44	44	CD
cord-009318-zt1o1bcz	547	28	)	)	-RRB-
cord-009318-zt1o1bcz	547	29	(	(	-LRB-
cord-009318-zt1o1bcz	547	30	45	45	CD
cord-009318-zt1o1bcz	547	31	)	)	-RRB-
cord-009318-zt1o1bcz	547	32	(	(	-LRB-
cord-009318-zt1o1bcz	547	33	46	46	CD
cord-009318-zt1o1bcz	547	34	)	)	-RRB-
cord-009318-zt1o1bcz	547	35	,	,	,
cord-009318-zt1o1bcz	547	36	with	with	IN
cord-009318-zt1o1bcz	547	37	applications	application	NNS
cord-009318-zt1o1bcz	547	38	that	that	WDT
cord-009318-zt1o1bcz	547	39	include	include	VBP
cord-009318-zt1o1bcz	547	40	among	among	IN
cord-009318-zt1o1bcz	547	41	others	other	NNS
cord-009318-zt1o1bcz	547	42	multiplexed	multiplexe	VBD
cord-009318-zt1o1bcz	547	43	pathogen	pathogen	NN
cord-009318-zt1o1bcz	547	44	identification	identification	NN
cord-009318-zt1o1bcz	547	45	and	and	CC
cord-009318-zt1o1bcz	547	46	antibiotic	antibiotic	JJ
cord-009318-zt1o1bcz	547	47	susceptibility	susceptibility	NN
cord-009318-zt1o1bcz	547	48	testing	testing	NN
cord-009318-zt1o1bcz	547	49	.	.	.
cord-009318-zt1o1bcz	548	1	Finally	finally	RB
cord-009318-zt1o1bcz	548	2	,	,	,
cord-009318-zt1o1bcz	548	3	studies	study	NNS
cord-009318-zt1o1bcz	548	4	with	with	IN
cord-009318-zt1o1bcz	548	5	clinical	clinical	JJ
cord-009318-zt1o1bcz	548	6	samples	sample	NNS
cord-009318-zt1o1bcz	548	7	should	should	MD
cord-009318-zt1o1bcz	548	8	be	be	VB
cord-009318-zt1o1bcz	548	9	performed	perform	VBN
cord-009318-zt1o1bcz	548	10	using	use	VBG
cord-009318-zt1o1bcz	548	11	the	the	DT
cord-009318-zt1o1bcz	548	12	dLAMP	dLAMP	NNS
cord-009318-zt1o1bcz	548	13	with	with	IN
cord-009318-zt1o1bcz	548	14	HRM	HRM	NNP
cord-009318-zt1o1bcz	548	15	method	method	NN
cord-009318-zt1o1bcz	548	16	to	to	TO
cord-009318-zt1o1bcz	548	17	understand	understand	VB
cord-009318-zt1o1bcz	548	18	the	the	DT
cord-009318-zt1o1bcz	548	19	carryover	carryover	NN
cord-009318-zt1o1bcz	548	20	effects	effect	NNS
cord-009318-zt1o1bcz	548	21	from	from	IN
cord-009318-zt1o1bcz	548	22	relevant	relevant	JJ
cord-009318-zt1o1bcz	548	23	matrices	matrix	NNS
cord-009318-zt1o1bcz	548	24	.	.	.
cord-009318-zt1o1bcz	549	1	The	the	DT
cord-009318-zt1o1bcz	549	2	complete	complete	JJ
cord-009318-zt1o1bcz	549	3	sequencing	sequencing	NN
cord-009318-zt1o1bcz	549	4	data	datum	NNS
cord-009318-zt1o1bcz	549	5	generated	generate	VBN
cord-009318-zt1o1bcz	549	6	during	during	IN
cord-009318-zt1o1bcz	549	7	this	this	DT
cord-009318-zt1o1bcz	549	8	study	study	NN
cord-009318-zt1o1bcz	549	9	are	be	VBP
cord-009318-zt1o1bcz	549	10	available	available	JJ
cord-009318-zt1o1bcz	549	11	in	in	IN
cord-009318-zt1o1bcz	549	12	the	the	DT
cord-009318-zt1o1bcz	549	13	National	National	NNP
cord-009318-zt1o1bcz	549	14	Center	Center	NNP
cord-009318-zt1o1bcz	549	15	for	for	IN
cord-009318-zt1o1bcz	549	16	Biotechnology	Biotechnology	NNP
cord-009318-zt1o1bcz	549	17	Information	Information	NNP
cord-009318-zt1o1bcz	549	18	Sequence	sequence	NN
cord-009318-zt1o1bcz	550	1	Read	read	VB
cord-009318-zt1o1bcz	550	2	Archive	Archive	NNP
cord-009318-zt1o1bcz	550	3	repository	repository	NN
cord-009318-zt1o1bcz	550	4	with	with	IN
cord-009318-zt1o1bcz	550	5	the	the	DT
cord-009318-zt1o1bcz	550	6	Bio	Bio	NNP
cord-009318-zt1o1bcz	550	7	-	-	HYPH
cord-009318-zt1o1bcz	550	8	Project	Project	NNP
cord-009318-zt1o1bcz	550	9	ID	ID	NNP
cord-009318-zt1o1bcz	550	10	:	:	:
cord-009318-zt1o1bcz	551	1	PRJNA574638	PRJNA574638	NNP
cord-009318-zt1o1bcz	551	2	.	.	.
cord-009318-zt1o1bcz	552	1	The	the	DT
cord-009318-zt1o1bcz	552	2	MATLAB	MATLAB	NNP
cord-009318-zt1o1bcz	552	3	script	script	NN
cord-009318-zt1o1bcz	552	4	described	describe	VBN
cord-009318-zt1o1bcz	552	5	here	here	RB
cord-009318-zt1o1bcz	552	6	has	have	VBZ
cord-009318-zt1o1bcz	552	7	been	be	VBN
cord-009318-zt1o1bcz	552	8	deposited	deposit	VBN
cord-009318-zt1o1bcz	552	9	in	in	IN
cord-009318-zt1o1bcz	552	10	the	the	DT
cord-009318-zt1o1bcz	552	11	open	open	JJ
cord-009318-zt1o1bcz	552	12	-	-	HYPH
cord-009318-zt1o1bcz	552	13	access	access	NN
cord-009318-zt1o1bcz	552	14	online	online	JJ
cord-009318-zt1o1bcz	552	15	repository	repository	NN
cord-009318-zt1o1bcz	552	16	GitHub	GitHub	NNP
cord-009318-zt1o1bcz	552	17	and	and	CC
cord-009318-zt1o1bcz	552	18	may	may	MD
cord-009318-zt1o1bcz	552	19	be	be	VB
cord-009318-zt1o1bcz	552	20	accessed	access	VBN
cord-009318-zt1o1bcz	552	21	using	use	VBG
cord-009318-zt1o1bcz	552	22	the	the	DT
cord-009318-zt1o1bcz	552	23	following	follow	VBG
cord-009318-zt1o1bcz	552	24	direct	direct	JJ
cord-009318-zt1o1bcz	552	25	link	link	NN
cord-009318-zt1o1bcz	552	26	:	:	:
cord-009318-zt1o1bcz	552	27	https://github.com/	https://github.com/	CD
cord-009318-zt1o1bcz	552	28	IsmagilovLab	IsmagilovLab	NNP
cord-009318-zt1o1bcz	552	29	/	/	SYM
cord-009318-zt1o1bcz	552	30	Digital	Digital	NNP
cord-009318-zt1o1bcz	552	31	NAAT	NAAT	NNP
cord-009318-zt1o1bcz	552	32	2Ch	2ch	CD
cord-009318-zt1o1bcz	552	33	MeltCurve	MeltCurve	NNP
cord-009318-zt1o1bcz	552	34	Analyzer	Analyzer	NNP
cord-009318-zt1o1bcz	552	35	.	.	.
cord-009318-zt1o1bcz	553	1	Highly	highly	RB
cord-009318-zt1o1bcz	553	2	stable	stable	JJ
cord-009318-zt1o1bcz	553	3	and	and	CC
cord-009318-zt1o1bcz	553	4	sensitive	sensitive	JJ
cord-009318-zt1o1bcz	553	5	nucleic	nucleic	JJ
cord-009318-zt1o1bcz	553	6	acid	acid	NN
cord-009318-zt1o1bcz	553	7	amplification	amplification	NN
cord-009318-zt1o1bcz	553	8	and	and	CC
cord-009318-zt1o1bcz	553	9	cell	cell	NN
cord-009318-zt1o1bcz	553	10	-	-	HYPH
cord-009318-zt1o1bcz	553	11	phone	phone	NN
cord-009318-zt1o1bcz	553	12	-	-	HYPH
cord-009318-zt1o1bcz	553	13	based	base	VBN
cord-009318-zt1o1bcz	553	14	readout	readout	NN
cord-009318-zt1o1bcz	553	15	Strand	strand	NN
cord-009318-zt1o1bcz	553	16	displacement	displacement	NN
cord-009318-zt1o1bcz	553	17	probes	probe	NNS
cord-009318-zt1o1bcz	553	18	combined	combine	VBN
cord-009318-zt1o1bcz	553	19	with	with	IN
cord-009318-zt1o1bcz	553	20	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	553	21	nucleic	nucleic	JJ
cord-009318-zt1o1bcz	553	22	acid	acid	NN
cord-009318-zt1o1bcz	553	23	amplification	amplification	NN
cord-009318-zt1o1bcz	553	24	for	for	IN
cord-009318-zt1o1bcz	553	25	instrument	instrument	NN
cord-009318-zt1o1bcz	553	26	-	-	HYPH
cord-009318-zt1o1bcz	553	27	free	free	JJ
cord-009318-zt1o1bcz	553	28	detection	detection	NN
cord-009318-zt1o1bcz	553	29	from	from	IN
cord-009318-zt1o1bcz	553	30	complex	complex	JJ
cord-009318-zt1o1bcz	553	31	samples	sample	NNS
cord-009318-zt1o1bcz	553	32	Loop	loop	RB
cord-009318-zt1o1bcz	553	33	-	-	HYPH
cord-009318-zt1o1bcz	553	34	mediated	mediate	VBN
cord-009318-zt1o1bcz	553	35	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	553	36	amplification	amplification	NN
cord-009318-zt1o1bcz	553	37	of	of	IN
cord-009318-zt1o1bcz	553	38	DNA	DNA	NNP
cord-009318-zt1o1bcz	553	39	Rapid	Rapid	NNP
cord-009318-zt1o1bcz	553	40	and	and	CC
cord-009318-zt1o1bcz	553	41	sensitive	sensitive	JJ
cord-009318-zt1o1bcz	553	42	detection	detection	NN
cord-009318-zt1o1bcz	553	43	of	of	IN
cord-009318-zt1o1bcz	553	44	Chlamydia	Chlamydia	NNP
cord-009318-zt1o1bcz	553	45	trachomatis	trachomatis	NN
cord-009318-zt1o1bcz	553	46	sexually	sexually	RB
cord-009318-zt1o1bcz	553	47	transmitted	transmit	VBN
cord-009318-zt1o1bcz	553	48	infections	infection	NNS
cord-009318-zt1o1bcz	553	49	in	in	IN
cord-009318-zt1o1bcz	553	50	resource	resource	NN
cord-009318-zt1o1bcz	553	51	-	-	HYPH
cord-009318-zt1o1bcz	553	52	constrained	constrain	VBN
cord-009318-zt1o1bcz	553	53	settings	setting	NNS
cord-009318-zt1o1bcz	553	54	in	in	IN
cord-009318-zt1o1bcz	553	55	Thailand	Thailand	NNP
cord-009318-zt1o1bcz	553	56	at	at	IN
cord-009318-zt1o1bcz	553	57	the	the	DT
cord-009318-zt1o1bcz	553	58	point	point	NN
cord-009318-zt1o1bcz	553	59	-	-	HYPH
cord-009318-zt1o1bcz	553	60	of	of	IN
cord-009318-zt1o1bcz	553	61	-	-	HYPH
cord-009318-zt1o1bcz	553	62	care	care	NN
cord-009318-zt1o1bcz	553	63	Chlamydia	Chlamydia	NNP
cord-009318-zt1o1bcz	553	64	trachomatis	trachomatis	NN
cord-009318-zt1o1bcz	553	65	and	and	CC
cord-009318-zt1o1bcz	553	66	genital	genital	JJ
cord-009318-zt1o1bcz	553	67	mycoplasmas	mycoplasmas	NN
cord-009318-zt1o1bcz	553	68	:	:	:
cord-009318-zt1o1bcz	553	69	pathogens	pathogen	NNS
cord-009318-zt1o1bcz	553	70	with	with	IN
cord-009318-zt1o1bcz	553	71	an	an	DT
cord-009318-zt1o1bcz	553	72	impact	impact	NN
cord-009318-zt1o1bcz	553	73	on	on	IN
cord-009318-zt1o1bcz	553	74	human	human	JJ
cord-009318-zt1o1bcz	553	75	reproductive	reproductive	JJ
cord-009318-zt1o1bcz	553	76	health	health	NN
cord-009318-zt1o1bcz	553	77	Diagnosis	Diagnosis	NNP
cord-009318-zt1o1bcz	553	78	of	of	IN
cord-009318-zt1o1bcz	553	79	Chlamydia	Chlamydia	NNP
cord-009318-zt1o1bcz	553	80	infection	infection	NN
cord-009318-zt1o1bcz	553	81	in	in	IN
cord-009318-zt1o1bcz	553	82	women	woman	NNS
cord-009318-zt1o1bcz	553	83	Supplemental	supplemental	JJ
cord-009318-zt1o1bcz	553	84	Tables	Tables	NNPS
cord-009318-zt1o1bcz	553	85	for	for	IN
cord-009318-zt1o1bcz	553	86	Interference	Interference	NNP
cord-009318-zt1o1bcz	553	87	Testing	Testing	NNP
cord-009318-zt1o1bcz	553	88	in	in	IN
cord-009318-zt1o1bcz	553	89	Clinical	Clinical	NNP
cord-009318-zt1o1bcz	553	90	Chemistry	Chemistry	NNP
cord-009318-zt1o1bcz	553	91	,	,	,
cord-009318-zt1o1bcz	553	92	1st	1st	NNP
cord-009318-zt1o1bcz	553	93	Edition	Edition	NNP
cord-009318-zt1o1bcz	553	94	,	,	,
cord-009318-zt1o1bcz	553	95	EP	EP	NNP
cord-009318-zt1o1bcz	553	96	37	37	CD
cord-009318-zt1o1bcz	553	97	Interference	Interference	NNP
cord-009318-zt1o1bcz	553	98	Testing	Testing	NNP
cord-009318-zt1o1bcz	553	99	in	in	IN
cord-009318-zt1o1bcz	553	100	Clinical	Clinical	NNP
cord-009318-zt1o1bcz	553	101	Chemistry	Chemistry	NNP
cord-009318-zt1o1bcz	553	102	Mathematical	Mathematical	NNP
cord-009318-zt1o1bcz	553	103	model	model	NN
cord-009318-zt1o1bcz	553	104	to	to	TO
cord-009318-zt1o1bcz	553	105	reduce	reduce	VB
cord-009318-zt1o1bcz	553	106	loop	loop	NN
cord-009318-zt1o1bcz	553	107	mediated	mediate	VBN
cord-009318-zt1o1bcz	553	108	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	553	109	amplification	amplification	NN
cord-009318-zt1o1bcz	553	110	(	(	-LRB-
cord-009318-zt1o1bcz	553	111	LAMP	lamp	NN
cord-009318-zt1o1bcz	553	112	)	)	-RRB-
cord-009318-zt1o1bcz	553	113	false	false	JJ
cord-009318-zt1o1bcz	553	114	-	-	HYPH
cord-009318-zt1o1bcz	553	115	positive	positive	JJ
cord-009318-zt1o1bcz	553	116	diagnosis	diagnosis	NN
cord-009318-zt1o1bcz	553	117	Real	real	JJ
cord-009318-zt1o1bcz	553	118	-	-	HYPH
cord-009318-zt1o1bcz	553	119	time	time	NN
cord-009318-zt1o1bcz	553	120	target	target	NN
cord-009318-zt1o1bcz	553	121	-	-	HYPH
cord-009318-zt1o1bcz	553	122	specific	specific	JJ
cord-009318-zt1o1bcz	553	123	detection	detection	NN
cord-009318-zt1o1bcz	553	124	of	of	IN
cord-009318-zt1o1bcz	553	125	loop	loop	NN
cord-009318-zt1o1bcz	553	126	-	-	HYPH
cord-009318-zt1o1bcz	553	127	mediated	mediate	VBN
cord-009318-zt1o1bcz	553	128	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	553	129	amplification	amplification	NN
cord-009318-zt1o1bcz	553	130	for	for	IN
cord-009318-zt1o1bcz	553	131	white	white	JJ
cord-009318-zt1o1bcz	553	132	spot	spot	NNP
cord-009318-zt1o1bcz	553	133	syndrome	syndrome	NN
cord-009318-zt1o1bcz	553	134	virus	virus	NN
cord-009318-zt1o1bcz	553	135	using	use	VBG
cord-009318-zt1o1bcz	553	136	fluorescence	fluorescence	NN
cord-009318-zt1o1bcz	553	137	energy	energy	NN
cord-009318-zt1o1bcz	553	138	transfer	transfer	NN
cord-009318-zt1o1bcz	553	139	-	-	HYPH
cord-009318-zt1o1bcz	553	140	based	base	VBN
cord-009318-zt1o1bcz	553	141	probes	probe	NNS
cord-009318-zt1o1bcz	553	142	Real	real	JJ
cord-009318-zt1o1bcz	553	143	-	-	HYPH
cord-009318-zt1o1bcz	553	144	time	time	NN
cord-009318-zt1o1bcz	553	145	detection	detection	NN
cord-009318-zt1o1bcz	553	146	and	and	CC
cord-009318-zt1o1bcz	553	147	monitoring	monitoring	NN
cord-009318-zt1o1bcz	553	148	of	of	IN
cord-009318-zt1o1bcz	553	149	loop	loop	NN
cord-009318-zt1o1bcz	553	150	mediated	mediate	VBN
cord-009318-zt1o1bcz	553	151	amplification	amplification	NN
cord-009318-zt1o1bcz	553	152	(	(	-LRB-
cord-009318-zt1o1bcz	553	153	LAMP	lamp	NN
cord-009318-zt1o1bcz	553	154	)	)	-RRB-
cord-009318-zt1o1bcz	553	155	reaction	reaction	NN
cord-009318-zt1o1bcz	553	156	using	use	VBG
cord-009318-zt1o1bcz	553	157	Self	self	NN
cord-009318-zt1o1bcz	553	158	-	-	HYPH
cord-009318-zt1o1bcz	553	159	quenching	quenching	NN
cord-009318-zt1o1bcz	553	160	and	and	CC
cord-009318-zt1o1bcz	553	161	De	de	JJ
cord-009318-zt1o1bcz	553	162	-	-	JJ
cord-009318-zt1o1bcz	553	163	quenching	quench	VBG
cord-009318-zt1o1bcz	553	164	fluorogenic	fluorogenic	JJ
cord-009318-zt1o1bcz	553	165	probes	probe	NNS
cord-009318-zt1o1bcz	553	166	Quenching	Quenching	NNP
cord-009318-zt1o1bcz	553	167	of	of	IN
cord-009318-zt1o1bcz	553	168	unincorporated	unincorporated	JJ
cord-009318-zt1o1bcz	553	169	amplification	amplification	NN
cord-009318-zt1o1bcz	553	170	signal	signal	NN
cord-009318-zt1o1bcz	553	171	reporters	reporter	NNS
cord-009318-zt1o1bcz	553	172	in	in	IN
cord-009318-zt1o1bcz	553	173	reverse	reverse	JJ
cord-009318-zt1o1bcz	553	174	-	-	HYPH
cord-009318-zt1o1bcz	553	175	transcription	transcription	NN
cord-009318-zt1o1bcz	553	176	loop	loop	NN
cord-009318-zt1o1bcz	553	177	-	-	HYPH
cord-009318-zt1o1bcz	553	178	mediated	mediate	VBN
cord-009318-zt1o1bcz	553	179	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	553	180	amplification	amplification	NN
cord-009318-zt1o1bcz	553	181	enabling	enable	VBG
cord-009318-zt1o1bcz	553	182	bright	bright	JJ
cord-009318-zt1o1bcz	553	183	,	,	,
cord-009318-zt1o1bcz	553	184	single	single	JJ
cord-009318-zt1o1bcz	553	185	-	-	HYPH
cord-009318-zt1o1bcz	553	186	step	step	NN
cord-009318-zt1o1bcz	553	187	,	,	,
cord-009318-zt1o1bcz	553	188	closed	closed	JJ
cord-009318-zt1o1bcz	553	189	-	-	HYPH
cord-009318-zt1o1bcz	553	190	tube	tube	NN
cord-009318-zt1o1bcz	553	191	,	,	,
cord-009318-zt1o1bcz	553	192	and	and	CC
cord-009318-zt1o1bcz	553	193	multiplexed	multiplexed	JJ
cord-009318-zt1o1bcz	553	194	detection	detection	NN
cord-009318-zt1o1bcz	553	195	of	of	IN
cord-009318-zt1o1bcz	553	196	RNA	RNA	NNP
cord-009318-zt1o1bcz	553	197	viruses	virus	NNS
cord-009318-zt1o1bcz	553	198	Simultaneous	simultaneous	JJ
cord-009318-zt1o1bcz	553	199	multiple	multiple	JJ
cord-009318-zt1o1bcz	553	200	target	target	NN
cord-009318-zt1o1bcz	553	201	detection	detection	NN
cord-009318-zt1o1bcz	553	202	in	in	IN
cord-009318-zt1o1bcz	553	203	real	real	JJ
cord-009318-zt1o1bcz	553	204	-	-	HYPH
cord-009318-zt1o1bcz	553	205	time	time	NN
cord-009318-zt1o1bcz	553	206	loop	loop	NN
cord-009318-zt1o1bcz	553	207	-	-	HYPH
cord-009318-zt1o1bcz	553	208	mediated	mediate	VBN
cord-009318-zt1o1bcz	553	209	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	553	210	amplification	amplification	NN
cord-009318-zt1o1bcz	553	211	Homogenous	homogenous	JJ
cord-009318-zt1o1bcz	553	212	,	,	,
cord-009318-zt1o1bcz	553	213	real	real	JJ
cord-009318-zt1o1bcz	553	214	-	-	HYPH
cord-009318-zt1o1bcz	553	215	time	time	NN
cord-009318-zt1o1bcz	553	216	duplex	duplex	JJ
cord-009318-zt1o1bcz	553	217	loop	loop	NN
cord-009318-zt1o1bcz	553	218	-	-	HYPH
cord-009318-zt1o1bcz	553	219	mediated	mediate	VBN
cord-009318-zt1o1bcz	553	220	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	553	221	amplification	amplification	NN
cord-009318-zt1o1bcz	553	222	using	use	VBG
cord-009318-zt1o1bcz	553	223	a	a	DT
cord-009318-zt1o1bcz	553	224	single	single	JJ
cord-009318-zt1o1bcz	553	225	fluorophore	fluorophore	NN
cord-009318-zt1o1bcz	553	226	-	-	HYPH
cord-009318-zt1o1bcz	553	227	labeled	label	VBN
cord-009318-zt1o1bcz	553	228	primer	primer	NN
cord-009318-zt1o1bcz	553	229	and	and	CC
cord-009318-zt1o1bcz	553	230	an	an	DT
cord-009318-zt1o1bcz	553	231	intercalator	intercalator	NN
cord-009318-zt1o1bcz	553	232	dye	dye	NN
cord-009318-zt1o1bcz	553	233	:	:	:
cord-009318-zt1o1bcz	553	234	Its	-PRON-	PRP$
cord-009318-zt1o1bcz	553	235	application	application	NN
cord-009318-zt1o1bcz	553	236	to	to	IN
cord-009318-zt1o1bcz	553	237	the	the	DT
cord-009318-zt1o1bcz	553	238	simultaneous	simultaneous	JJ
cord-009318-zt1o1bcz	553	239	detection	detection	NN
cord-009318-zt1o1bcz	553	240	of	of	IN
cord-009318-zt1o1bcz	553	241	Shiga	Shiga	NNP
cord-009318-zt1o1bcz	553	242	toxin	toxin	NN
cord-009318-zt1o1bcz	553	243	genes	gene	NNS
cord-009318-zt1o1bcz	553	244	1	1	CD
cord-009318-zt1o1bcz	553	245	and	and	CC
cord-009318-zt1o1bcz	553	246	2	2	CD
cord-009318-zt1o1bcz	553	247	in	in	IN
cord-009318-zt1o1bcz	553	248	Shiga	Shiga	NNP
cord-009318-zt1o1bcz	553	249	toxigenic	toxigenic	NNP
cord-009318-zt1o1bcz	553	250	Escherichia	escherichia	NN
cord-009318-zt1o1bcz	553	251	coli	coli	NNS
cord-009318-zt1o1bcz	553	252	isolates	isolate	NNS
cord-009318-zt1o1bcz	553	253	FRET	fret	NN
cord-009318-zt1o1bcz	553	254	-	-	HYPH
cord-009318-zt1o1bcz	553	255	based	base	VBN
cord-009318-zt1o1bcz	553	256	assimilating	assimilate	VBG
cord-009318-zt1o1bcz	553	257	probe	probe	NN
cord-009318-zt1o1bcz	553	258	for	for	IN
cord-009318-zt1o1bcz	553	259	sequence	sequence	NN
cord-009318-zt1o1bcz	553	260	-	-	HYPH
cord-009318-zt1o1bcz	553	261	specific	specific	JJ
cord-009318-zt1o1bcz	553	262	real	real	JJ
cord-009318-zt1o1bcz	553	263	-	-	HYPH
cord-009318-zt1o1bcz	553	264	time	time	NN
cord-009318-zt1o1bcz	553	265	monitoring	monitoring	NN
cord-009318-zt1o1bcz	553	266	of	of	IN
cord-009318-zt1o1bcz	553	267	loop	loop	NN
cord-009318-zt1o1bcz	553	268	-	-	HYPH
cord-009318-zt1o1bcz	553	269	mediated	mediate	VBN
cord-009318-zt1o1bcz	553	270	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	553	271	amplification	amplification	NN
cord-009318-zt1o1bcz	553	272	(	(	-LRB-
cord-009318-zt1o1bcz	553	273	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	553	274	)	)	-RRB-
cord-009318-zt1o1bcz	553	275	Robust	robust	JJ
cord-009318-zt1o1bcz	553	276	strand	strand	NN
cord-009318-zt1o1bcz	553	277	exchange	exchange	NN
cord-009318-zt1o1bcz	553	278	reactions	reaction	NNS
cord-009318-zt1o1bcz	553	279	for	for	IN
cord-009318-zt1o1bcz	553	280	the	the	DT
cord-009318-zt1o1bcz	553	281	sequence	sequence	NN
cord-009318-zt1o1bcz	553	282	-	-	HYPH
cord-009318-zt1o1bcz	553	283	specific	specific	JJ
cord-009318-zt1o1bcz	553	284	,	,	,
cord-009318-zt1o1bcz	553	285	real	real	JJ
cord-009318-zt1o1bcz	553	286	-	-	HYPH
cord-009318-zt1o1bcz	553	287	time	time	NN
cord-009318-zt1o1bcz	553	288	detection	detection	NN
cord-009318-zt1o1bcz	553	289	of	of	IN
cord-009318-zt1o1bcz	553	290	nucleic	nucleic	JJ
cord-009318-zt1o1bcz	553	291	acid	acid	NN
cord-009318-zt1o1bcz	553	292	amplicons	amplicons	NNP
cord-009318-zt1o1bcz	553	293	Establishment	Establishment	NNP
cord-009318-zt1o1bcz	553	294	of	of	IN
cord-009318-zt1o1bcz	553	295	an	an	DT
cord-009318-zt1o1bcz	553	296	accurate	accurate	JJ
cord-009318-zt1o1bcz	553	297	and	and	CC
cord-009318-zt1o1bcz	553	298	fast	fast	JJ
cord-009318-zt1o1bcz	553	299	detection	detection	NN
cord-009318-zt1o1bcz	553	300	method	method	NN
cord-009318-zt1o1bcz	553	301	using	use	VBG
cord-009318-zt1o1bcz	553	302	molecular	molecular	JJ
cord-009318-zt1o1bcz	553	303	beacons	beacon	NNS
cord-009318-zt1o1bcz	553	304	in	in	IN
cord-009318-zt1o1bcz	553	305	loop	loop	NN
cord-009318-zt1o1bcz	553	306	-	-	HYPH
cord-009318-zt1o1bcz	553	307	mediated	mediate	VBN
cord-009318-zt1o1bcz	553	308	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	553	309	amplification	amplification	NN
cord-009318-zt1o1bcz	553	310	assay	assay	NN
cord-009318-zt1o1bcz	553	311	Real	real	JJ
cord-009318-zt1o1bcz	553	312	-	-	HYPH
cord-009318-zt1o1bcz	553	313	time	time	NN
cord-009318-zt1o1bcz	553	314	sequence	sequence	NN
cord-009318-zt1o1bcz	553	315	-	-	HYPH
cord-009318-zt1o1bcz	553	316	validated	validate	VBN
cord-009318-zt1o1bcz	553	317	loop	loop	NN
cord-009318-zt1o1bcz	553	318	-	-	HYPH
cord-009318-zt1o1bcz	553	319	mediated	mediate	VBN
cord-009318-zt1o1bcz	553	320	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	553	321	amplification	amplification	NN
cord-009318-zt1o1bcz	553	322	assays	assay	NNS
cord-009318-zt1o1bcz	553	323	for	for	IN
cord-009318-zt1o1bcz	553	324	detection	detection	NN
cord-009318-zt1o1bcz	553	325	of	of	IN
cord-009318-zt1o1bcz	553	326	middle	middle	NNP
cord-009318-zt1o1bcz	553	327	east	east	NNP
cord-009318-zt1o1bcz	553	328	respiratory	respiratory	JJ
cord-009318-zt1o1bcz	553	329	syndrome	syndrome	NN
cord-009318-zt1o1bcz	553	330	coronavirus	coronavirus	NN
cord-009318-zt1o1bcz	553	331	(	(	-LRB-
cord-009318-zt1o1bcz	553	332	MERS	MERS	NNP
cord-009318-zt1o1bcz	553	333	-	-	HYPH
cord-009318-zt1o1bcz	553	334	CoV	CoV	NNP
cord-009318-zt1o1bcz	553	335	)	)	-RRB-
cord-009318-zt1o1bcz	554	1	Adapting	adapt	VBG
cord-009318-zt1o1bcz	554	2	enzyme	enzyme	NN
cord-009318-zt1o1bcz	554	3	-	-	HYPH
cord-009318-zt1o1bcz	554	4	free	free	JJ
cord-009318-zt1o1bcz	554	5	DNA	dna	NN
cord-009318-zt1o1bcz	554	6	circuits	circuit	NNS
cord-009318-zt1o1bcz	554	7	to	to	IN
cord-009318-zt1o1bcz	554	8	the	the	DT
cord-009318-zt1o1bcz	554	9	detection	detection	NN
cord-009318-zt1o1bcz	554	10	of	of	IN
cord-009318-zt1o1bcz	554	11	loop	loop	NN
cord-009318-zt1o1bcz	554	12	-	-	HYPH
cord-009318-zt1o1bcz	554	13	mediated	mediate	VBN
cord-009318-zt1o1bcz	554	14	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	554	15	amplification	amplification	NN
cord-009318-zt1o1bcz	554	16	reactions	reaction	NNS
cord-009318-zt1o1bcz	554	17	A	a	DT
cord-009318-zt1o1bcz	554	18	digital	digital	JJ
cord-009318-zt1o1bcz	554	19	microfluidic	microfluidic	JJ
cord-009318-zt1o1bcz	554	20	system	system	NN
cord-009318-zt1o1bcz	554	21	for	for	IN
cord-009318-zt1o1bcz	554	22	loop	loop	NN
cord-009318-zt1o1bcz	554	23	-	-	HYPH
cord-009318-zt1o1bcz	554	24	mediated	mediate	VBN
cord-009318-zt1o1bcz	554	25	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	554	26	amplification	amplification	NN
cord-009318-zt1o1bcz	554	27	and	and	CC
cord-009318-zt1o1bcz	554	28	sequence	sequence	NN
cord-009318-zt1o1bcz	555	1	specific	specific	JJ
cord-009318-zt1o1bcz	555	2	pathogen	pathogen	NN
cord-009318-zt1o1bcz	555	3	detection	detection	NN
cord-009318-zt1o1bcz	555	4	Increased	increase	VBN
cord-009318-zt1o1bcz	555	5	robustness	robustness	NN
cord-009318-zt1o1bcz	555	6	of	of	IN
cord-009318-zt1o1bcz	555	7	single	single	JJ
cord-009318-zt1o1bcz	555	8	-	-	HYPH
cord-009318-zt1o1bcz	555	9	molecule	molecule	NN
cord-009318-zt1o1bcz	555	10	counting	counting	NN
cord-009318-zt1o1bcz	555	11	with	with	IN
cord-009318-zt1o1bcz	555	12	microfluidics	microfluidic	NNS
cord-009318-zt1o1bcz	555	13	,	,	,
cord-009318-zt1o1bcz	555	14	digital	digital	JJ
cord-009318-zt1o1bcz	555	15	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	555	16	amplification	amplification	NN
cord-009318-zt1o1bcz	555	17	,	,	,
cord-009318-zt1o1bcz	555	18	and	and	CC
cord-009318-zt1o1bcz	555	19	a	a	DT
cord-009318-zt1o1bcz	555	20	mobile	mobile	JJ
cord-009318-zt1o1bcz	555	21	phone	phone	NN
cord-009318-zt1o1bcz	555	22	versus	versus	IN
cord-009318-zt1o1bcz	555	23	real	real	JJ
cord-009318-zt1o1bcz	555	24	-	-	HYPH
cord-009318-zt1o1bcz	555	25	time	time	NN
cord-009318-zt1o1bcz	555	26	kinetic	kinetic	JJ
cord-009318-zt1o1bcz	555	27	measurements	measurement	NNS
cord-009318-zt1o1bcz	556	1	Mechanistic	mechanistic	JJ
cord-009318-zt1o1bcz	556	2	evaluation	evaluation	NN
cord-009318-zt1o1bcz	556	3	of	of	IN
cord-009318-zt1o1bcz	556	4	the	the	DT
cord-009318-zt1o1bcz	556	5	pros	pro	NNS
cord-009318-zt1o1bcz	556	6	and	and	CC
cord-009318-zt1o1bcz	556	7	cons	con	NNS
cord-009318-zt1o1bcz	556	8	of	of	IN
cord-009318-zt1o1bcz	556	9	digital	digital	JJ
cord-009318-zt1o1bcz	556	10	RT	RT	NNP
cord-009318-zt1o1bcz	556	11	-	-	HYPH
cord-009318-zt1o1bcz	556	12	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	556	13	for	for	IN
cord-009318-zt1o1bcz	556	14	HIV-1	HIV-1	NNP
cord-009318-zt1o1bcz	556	15	viral	viral	JJ
cord-009318-zt1o1bcz	556	16	load	load	NN
cord-009318-zt1o1bcz	556	17	quantification	quantification	NN
cord-009318-zt1o1bcz	556	18	on	on	IN
cord-009318-zt1o1bcz	556	19	a	a	DT
cord-009318-zt1o1bcz	556	20	microfluidic	microfluidic	JJ
cord-009318-zt1o1bcz	556	21	device	device	NN
cord-009318-zt1o1bcz	556	22	and	and	CC
cord-009318-zt1o1bcz	556	23	improved	improved	JJ
cord-009318-zt1o1bcz	556	24	efficiency	efficiency	NN
cord-009318-zt1o1bcz	556	25	via	via	IN
cord-009318-zt1o1bcz	556	26	a	a	DT
cord-009318-zt1o1bcz	556	27	two	two	CD
cord-009318-zt1o1bcz	556	28	-	-	HYPH
cord-009318-zt1o1bcz	556	29	step	step	NN
cord-009318-zt1o1bcz	556	30	digital	digital	JJ
cord-009318-zt1o1bcz	556	31	protocol	protocol	NN
cord-009318-zt1o1bcz	556	32	Real	real	JJ
cord-009318-zt1o1bcz	556	33	-	-	HYPH
cord-009318-zt1o1bcz	556	34	time	time	NN
cord-009318-zt1o1bcz	556	35	,	,	,
cord-009318-zt1o1bcz	556	36	digital	digital	JJ
cord-009318-zt1o1bcz	556	37	lamp	lamp	NN
cord-009318-zt1o1bcz	556	38	with	with	IN
cord-009318-zt1o1bcz	556	39	commercial	commercial	JJ
cord-009318-zt1o1bcz	556	40	microfluidic	microfluidic	JJ
cord-009318-zt1o1bcz	556	41	chips	chip	NNS
cord-009318-zt1o1bcz	556	42	reveals	reveal	VBZ
cord-009318-zt1o1bcz	556	43	the	the	DT
cord-009318-zt1o1bcz	556	44	interplay	interplay	NN
cord-009318-zt1o1bcz	556	45	of	of	IN
cord-009318-zt1o1bcz	556	46	efficiency	efficiency	NN
cord-009318-zt1o1bcz	556	47	,	,	,
cord-009318-zt1o1bcz	556	48	speed	speed	NN
cord-009318-zt1o1bcz	556	49	,	,	,
cord-009318-zt1o1bcz	556	50	and	and	CC
cord-009318-zt1o1bcz	556	51	background	background	NN
cord-009318-zt1o1bcz	556	52	amplification	amplification	NN
cord-009318-zt1o1bcz	556	53	as	as	IN
cord-009318-zt1o1bcz	556	54	a	a	DT
cord-009318-zt1o1bcz	556	55	function	function	NN
cord-009318-zt1o1bcz	556	56	of	of	IN
cord-009318-zt1o1bcz	556	57	reaction	reaction	NN
cord-009318-zt1o1bcz	556	58	temperature	temperature	NN
cord-009318-zt1o1bcz	556	59	and	and	CC
cord-009318-zt1o1bcz	556	60	time	time	NN
cord-009318-zt1o1bcz	556	61	Instrument	instrument	NN
cord-009318-zt1o1bcz	556	62	for	for	IN
cord-009318-zt1o1bcz	556	63	real	real	JJ
cord-009318-zt1o1bcz	556	64	-	-	HYPH
cord-009318-zt1o1bcz	556	65	time	time	NN
cord-009318-zt1o1bcz	556	66	digital	digital	JJ
cord-009318-zt1o1bcz	556	67	nucleic	nucleic	JJ
cord-009318-zt1o1bcz	556	68	acid	acid	NN
cord-009318-zt1o1bcz	556	69	amplification	amplification	NN
cord-009318-zt1o1bcz	556	70	on	on	IN
cord-009318-zt1o1bcz	556	71	custom	custom	NN
cord-009318-zt1o1bcz	556	72	microfluidic	microfluidic	JJ
cord-009318-zt1o1bcz	556	73	devices	device	NNS
cord-009318-zt1o1bcz	556	74	Loop	loop	RB
cord-009318-zt1o1bcz	556	75	-	-	HYPH
cord-009318-zt1o1bcz	556	76	mediated	mediate	VBN
cord-009318-zt1o1bcz	556	77	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	556	78	amplification	amplification	NN
cord-009318-zt1o1bcz	556	79	(	(	-LRB-
cord-009318-zt1o1bcz	556	80	LAMP	lamp	NN
cord-009318-zt1o1bcz	556	81	)	)	-RRB-
cord-009318-zt1o1bcz	556	82	method	method	NN
cord-009318-zt1o1bcz	556	83	for	for	IN
cord-009318-zt1o1bcz	556	84	rapid	rapid	JJ
cord-009318-zt1o1bcz	556	85	detection	detection	NN
cord-009318-zt1o1bcz	556	86	of	of	IN
cord-009318-zt1o1bcz	556	87	Trypanosoma	Trypanosoma	NNP
cord-009318-zt1o1bcz	556	88	brucei	brucei	NNP
cord-009318-zt1o1bcz	556	89	rhodesiense	rhodesiense	NN
cord-009318-zt1o1bcz	556	90	Enhancing	enhance	VBG
cord-009318-zt1o1bcz	556	91	melting	melt	VBG
cord-009318-zt1o1bcz	556	92	curve	curve	NN
cord-009318-zt1o1bcz	556	93	analysis	analysis	NN
cord-009318-zt1o1bcz	556	94	for	for	IN
cord-009318-zt1o1bcz	556	95	the	the	DT
cord-009318-zt1o1bcz	556	96	discrimination	discrimination	NN
cord-009318-zt1o1bcz	556	97	of	of	IN
cord-009318-zt1o1bcz	556	98	loop	loop	NN
cord-009318-zt1o1bcz	556	99	-	-	HYPH
cord-009318-zt1o1bcz	556	100	mediated	mediate	VBN
cord-009318-zt1o1bcz	556	101	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	556	102	amplification	amplification	NN
cord-009318-zt1o1bcz	556	103	products	product	NNS
cord-009318-zt1o1bcz	556	104	from	from	IN
cord-009318-zt1o1bcz	556	105	four	four	CD
cord-009318-zt1o1bcz	556	106	pathogenic	pathogenic	JJ
cord-009318-zt1o1bcz	556	107	molds	mold	NNS
cord-009318-zt1o1bcz	556	108	:	:	:
cord-009318-zt1o1bcz	556	109	Use	Use	NNP
cord-009318-zt1o1bcz	556	110	of	of	IN
cord-009318-zt1o1bcz	556	111	inorganic	inorganic	JJ
cord-009318-zt1o1bcz	556	112	pyrophosphatase	pyrophosphatase	NN
cord-009318-zt1o1bcz	556	113	and	and	CC
cord-009318-zt1o1bcz	556	114	its	-PRON-	PRP$
cord-009318-zt1o1bcz	556	115	effect	effect	NN
cord-009318-zt1o1bcz	556	116	in	in	IN
cord-009318-zt1o1bcz	556	117	reducing	reduce	VBG
cord-009318-zt1o1bcz	556	118	the	the	DT
cord-009318-zt1o1bcz	556	119	variance	variance	NN
cord-009318-zt1o1bcz	556	120	in	in	IN
cord-009318-zt1o1bcz	556	121	melting	melt	VBG
cord-009318-zt1o1bcz	556	122	temperature	temperature	NN
cord-009318-zt1o1bcz	556	123	values	value	NNS
cord-009318-zt1o1bcz	556	124	Development	Development	NNP
cord-009318-zt1o1bcz	556	125	of	of	IN
cord-009318-zt1o1bcz	556	126	a	a	DT
cord-009318-zt1o1bcz	556	127	loop	loop	NN
cord-009318-zt1o1bcz	556	128	-	-	HYPH
cord-009318-zt1o1bcz	556	129	mediated	mediate	VBN
cord-009318-zt1o1bcz	556	130	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	556	131	amplification	amplification	NN
cord-009318-zt1o1bcz	556	132	method	method	NN
cord-009318-zt1o1bcz	556	133	for	for	IN
cord-009318-zt1o1bcz	556	134	diagnosing	diagnose	VBG
cord-009318-zt1o1bcz	556	135	Pneumocystis	Pneumocystis	NNP
cord-009318-zt1o1bcz	556	136	pneumonia	pneumonia	NN
cord-009318-zt1o1bcz	556	137	Development	Development	NNP
cord-009318-zt1o1bcz	556	138	of	of	IN
cord-009318-zt1o1bcz	556	139	a	a	DT
cord-009318-zt1o1bcz	556	140	multiplex	multiplex	JJ
cord-009318-zt1o1bcz	556	141	loop	loop	NN
cord-009318-zt1o1bcz	556	142	-	-	HYPH
cord-009318-zt1o1bcz	556	143	mediated	mediate	VBN
cord-009318-zt1o1bcz	556	144	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	556	145	amplification	amplification	NN
cord-009318-zt1o1bcz	556	146	method	method	NN
cord-009318-zt1o1bcz	556	147	for	for	IN
cord-009318-zt1o1bcz	556	148	the	the	DT
cord-009318-zt1o1bcz	556	149	simultaneous	simultaneous	JJ
cord-009318-zt1o1bcz	556	150	detection	detection	NN
cord-009318-zt1o1bcz	556	151	of	of	IN
cord-009318-zt1o1bcz	556	152	Salmonella	Salmonella	NNP
cord-009318-zt1o1bcz	556	153	spp	spp	NNP
cord-009318-zt1o1bcz	556	154	.	.	.
cord-009318-zt1o1bcz	557	1	and	and	CC
cord-009318-zt1o1bcz	557	2	Vibrio	Vibrio	NNP
cord-009318-zt1o1bcz	557	3	parahaemolyticus	parahaemolyticus	NNP
cord-009318-zt1o1bcz	557	4	Novel	Novel	NNP
cord-009318-zt1o1bcz	557	5	loop	loop	NN
cord-009318-zt1o1bcz	557	6	-	-	HYPH
cord-009318-zt1o1bcz	557	7	mediated	mediate	VBN
cord-009318-zt1o1bcz	557	8	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	557	9	amplification	amplification	NN
cord-009318-zt1o1bcz	557	10	(	(	-LRB-
cord-009318-zt1o1bcz	557	11	LAMP	lamp	NN
cord-009318-zt1o1bcz	557	12	)	)	-RRB-
cord-009318-zt1o1bcz	557	13	assay	assay	NN
cord-009318-zt1o1bcz	557	14	with	with	IN
cord-009318-zt1o1bcz	557	15	a	a	DT
cord-009318-zt1o1bcz	557	16	universal	universal	JJ
cord-009318-zt1o1bcz	557	17	QProbe	QProbe	NNP
cord-009318-zt1o1bcz	557	18	can	can	MD
cord-009318-zt1o1bcz	557	19	detect	detect	VB
cord-009318-zt1o1bcz	557	20	SNPs	snp	NNS
cord-009318-zt1o1bcz	557	21	determining	determine	VBG
cord-009318-zt1o1bcz	557	22	races	race	NNS
cord-009318-zt1o1bcz	557	23	in	in	IN
cord-009318-zt1o1bcz	557	24	plant	plant	NN
cord-009318-zt1o1bcz	557	25	pathogenic	pathogenic	JJ
cord-009318-zt1o1bcz	557	26	fungi	fungus	NNS
cord-009318-zt1o1bcz	557	27	Isothermal	Isothermal	NNP
cord-009318-zt1o1bcz	557	28	amplification	amplification	NN
cord-009318-zt1o1bcz	557	29	and	and	CC
cord-009318-zt1o1bcz	557	30	multimerization	multimerization	NN
cord-009318-zt1o1bcz	557	31	of	of	IN
cord-009318-zt1o1bcz	557	32	DNA	DNA	NNP
cord-009318-zt1o1bcz	557	33	by	by	IN
cord-009318-zt1o1bcz	557	34	Bst	Bst	NNP
cord-009318-zt1o1bcz	557	35	DNA	DNA	NNP
cord-009318-zt1o1bcz	557	36	polymerase	polymerase	NN
cord-009318-zt1o1bcz	557	37	Unusual	unusual	JJ
cord-009318-zt1o1bcz	557	38	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	557	39	multimerization	multimerization	NN
cord-009318-zt1o1bcz	557	40	and	and	CC
cord-009318-zt1o1bcz	557	41	amplification	amplification	NN
cord-009318-zt1o1bcz	557	42	by	by	IN
cord-009318-zt1o1bcz	557	43	the	the	DT
cord-009318-zt1o1bcz	557	44	strand	strand	NN
cord-009318-zt1o1bcz	557	45	-	-	HYPH
cord-009318-zt1o1bcz	557	46	displacing	displace	VBG
cord-009318-zt1o1bcz	557	47	DNA	dna	NN
cord-009318-zt1o1bcz	557	48	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	557	49	with	with	IN
cord-009318-zt1o1bcz	557	50	reverse	reverse	JJ
cord-009318-zt1o1bcz	557	51	transcription	transcription	NN
cord-009318-zt1o1bcz	557	52	activities	activity	NNS
cord-009318-zt1o1bcz	558	1	N.BspD6I	N.BspD6I	NNP
cord-009318-zt1o1bcz	558	2	DNA	DNA	NNP
cord-009318-zt1o1bcz	558	3	nickase	nickase	NN
cord-009318-zt1o1bcz	558	4	strongly	strongly	RB
cord-009318-zt1o1bcz	558	5	stimulates	stimulate	VBZ
cord-009318-zt1o1bcz	558	6	template	template	NN
cord-009318-zt1o1bcz	558	7	-	-	HYPH
cord-009318-zt1o1bcz	558	8	independent	independent	JJ
cord-009318-zt1o1bcz	558	9	synthesis	synthesis	NN
cord-009318-zt1o1bcz	558	10	of	of	IN
cord-009318-zt1o1bcz	558	11	non	non	JJ
cord-009318-zt1o1bcz	558	12	-	-	JJ
cord-009318-zt1o1bcz	558	13	palindromic	palindromic	JJ
cord-009318-zt1o1bcz	558	14	repetitive	repetitive	JJ
cord-009318-zt1o1bcz	558	15	DNA	dna	NN
cord-009318-zt1o1bcz	558	16	by	by	IN
cord-009318-zt1o1bcz	558	17	Bst	Bst	NNP
cord-009318-zt1o1bcz	558	18	DNA	DNA	NNP
cord-009318-zt1o1bcz	558	19	polymerase	polymerase	NN
cord-009318-zt1o1bcz	558	20	Analysis	analysis	NN
cord-009318-zt1o1bcz	558	21	of	of	IN
cord-009318-zt1o1bcz	558	22	non	non	JJ
cord-009318-zt1o1bcz	558	23	-	-	JJ
cord-009318-zt1o1bcz	558	24	template	template	NN
cord-009318-zt1o1bcz	558	25	-	-	HYPH
cord-009318-zt1o1bcz	558	26	directed	direct	VBN
cord-009318-zt1o1bcz	558	27	nucleotide	nucleotide	JJ
cord-009318-zt1o1bcz	558	28	addition	addition	NN
cord-009318-zt1o1bcz	558	29	and	and	CC
cord-009318-zt1o1bcz	558	30	template	template	NN
cord-009318-zt1o1bcz	558	31	switching	switch	VBG
cord-009318-zt1o1bcz	558	32	by	by	IN
cord-009318-zt1o1bcz	558	33	DNA	dna	NN
cord-009318-zt1o1bcz	558	34	polymerase	polymerase	NN
cord-009318-zt1o1bcz	558	35	De	De	NNP
cord-009318-zt1o1bcz	558	36	novo	novo	NNP
cord-009318-zt1o1bcz	558	37	DNA	DNA	NNP
cord-009318-zt1o1bcz	558	38	synthesis	synthesis	NN
cord-009318-zt1o1bcz	558	39	by	by	IN
cord-009318-zt1o1bcz	558	40	human	human	JJ
cord-009318-zt1o1bcz	558	41	DNA	dna	NN
cord-009318-zt1o1bcz	558	42	polymerase	polymerase	NN
cord-009318-zt1o1bcz	558	43	,	,	,
cord-009318-zt1o1bcz	558	44	DNA	dna	NN
cord-009318-zt1o1bcz	558	45	polymerase	polymerase	NN
cord-009318-zt1o1bcz	558	46	and	and	CC
cord-009318-zt1o1bcz	558	47	terminal	terminal	JJ
cord-009318-zt1o1bcz	558	48	deoxyribonucleotidyl	deoxyribonucleotidyl	NNP
cord-009318-zt1o1bcz	558	49	transferase	transferase	NNP
cord-009318-zt1o1bcz	558	50	Ab	Ab	NNP
cord-009318-zt1o1bcz	558	51	initio	initio	JJ
cord-009318-zt1o1bcz	558	52	synthesis	synthesis	NN
cord-009318-zt1o1bcz	558	53	by	by	IN
cord-009318-zt1o1bcz	558	54	DNA	dna	NN
cord-009318-zt1o1bcz	558	55	polymerases	polymerase	NNS
cord-009318-zt1o1bcz	558	56	Interrogation	Interrogation	NNP
cord-009318-zt1o1bcz	558	57	of	of	IN
cord-009318-zt1o1bcz	558	58	multimeric	multimeric	JJ
cord-009318-zt1o1bcz	558	59	DNA	dna	NN
cord-009318-zt1o1bcz	558	60	amplification	amplification	NN
cord-009318-zt1o1bcz	558	61	products	product	NNS
cord-009318-zt1o1bcz	558	62	by	by	IN
cord-009318-zt1o1bcz	558	63	competitive	competitive	JJ
cord-009318-zt1o1bcz	558	64	primer	primer	NN
cord-009318-zt1o1bcz	558	65	extension	extension	NN
cord-009318-zt1o1bcz	558	66	using	use	VBG
cord-009318-zt1o1bcz	558	67	Bst	Bst	NNP
cord-009318-zt1o1bcz	558	68	DNA	dna	NN
cord-009318-zt1o1bcz	558	69	polymerase	polymerase	NN
cord-009318-zt1o1bcz	558	70	(	(	-LRB-
cord-009318-zt1o1bcz	558	71	Large	large	JJ
cord-009318-zt1o1bcz	558	72	Fragment	Fragment	NNP
cord-009318-zt1o1bcz	558	73	)	)	-RRB-
cord-009318-zt1o1bcz	558	74	Impact	impact	NN
cord-009318-zt1o1bcz	558	75	of	of	IN
cord-009318-zt1o1bcz	558	76	primer	primer	NN
cord-009318-zt1o1bcz	558	77	dimers	dimer	NNS
cord-009318-zt1o1bcz	558	78	and	and	CC
cord-009318-zt1o1bcz	558	79	self	self	NN
cord-009318-zt1o1bcz	558	80	-	-	HYPH
cord-009318-zt1o1bcz	558	81	amplifying	amplify	VBG
cord-009318-zt1o1bcz	558	82	hairpins	hairpin	NNS
cord-009318-zt1o1bcz	558	83	on	on	IN
cord-009318-zt1o1bcz	558	84	reverse	reverse	JJ
cord-009318-zt1o1bcz	558	85	transcription	transcription	NN
cord-009318-zt1o1bcz	558	86	loop	loop	NN
cord-009318-zt1o1bcz	558	87	-	-	HYPH
cord-009318-zt1o1bcz	558	88	mediated	mediate	VBN
cord-009318-zt1o1bcz	558	89	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	558	90	amplification	amplification	NN
cord-009318-zt1o1bcz	558	91	detection	detection	NN
cord-009318-zt1o1bcz	558	92	of	of	IN
cord-009318-zt1o1bcz	558	93	viral	viral	JJ
cord-009318-zt1o1bcz	558	94	RNA	RNA	NNP
cord-009318-zt1o1bcz	558	95	Lack	Lack	NNP
cord-009318-zt1o1bcz	558	96	of	of	IN
cord-009318-zt1o1bcz	558	97	correlation	correlation	NN
cord-009318-zt1o1bcz	558	98	between	between	IN
cord-009318-zt1o1bcz	558	99	reaction	reaction	NN
cord-009318-zt1o1bcz	558	100	speed	speed	NN
cord-009318-zt1o1bcz	558	101	and	and	CC
cord-009318-zt1o1bcz	558	102	analytical	analytical	JJ
cord-009318-zt1o1bcz	558	103	sensitivity	sensitivity	NN
cord-009318-zt1o1bcz	558	104	in	in	IN
cord-009318-zt1o1bcz	558	105	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	558	106	amplification	amplification	NN
cord-009318-zt1o1bcz	558	107	reveals	reveal	VBZ
cord-009318-zt1o1bcz	558	108	the	the	DT
cord-009318-zt1o1bcz	558	109	value	value	NN
cord-009318-zt1o1bcz	558	110	of	of	IN
cord-009318-zt1o1bcz	558	111	digital	digital	JJ
cord-009318-zt1o1bcz	558	112	methods	method	NNS
cord-009318-zt1o1bcz	558	113	for	for	IN
cord-009318-zt1o1bcz	558	114	optimization	optimization	NN
cord-009318-zt1o1bcz	558	115	:	:	:
cord-009318-zt1o1bcz	558	116	validation	validation	NN
cord-009318-zt1o1bcz	558	117	using	use	VBG
cord-009318-zt1o1bcz	558	118	digital	digital	JJ
cord-009318-zt1o1bcz	558	119	real	real	JJ
cord-009318-zt1o1bcz	558	120	-	-	HYPH
cord-009318-zt1o1bcz	558	121	time	time	NN
cord-009318-zt1o1bcz	558	122	RT	RT	NNP
cord-009318-zt1o1bcz	558	123	-	-	HYPH
cord-009318-zt1o1bcz	558	124	LAMP	LAMP	NNP
cord-009318-zt1o1bcz	558	125	Loop	Loop	NNP
cord-009318-zt1o1bcz	558	126	-	-	HYPH
cord-009318-zt1o1bcz	558	127	mediated	mediate	VBN
cord-009318-zt1o1bcz	558	128	isothermal	isothermal	JJ
cord-009318-zt1o1bcz	558	129	amplification	amplification	NN
cord-009318-zt1o1bcz	558	130	for	for	IN
cord-009318-zt1o1bcz	558	131	detection	detection	NN
cord-009318-zt1o1bcz	558	132	of	of	IN
cord-009318-zt1o1bcz	558	133	nucleic	nucleic	JJ
cord-009318-zt1o1bcz	558	134	acids	acid	NNS
cord-009318-zt1o1bcz	558	135	Single	single	JJ
cord-009318-zt1o1bcz	558	136	-	-	HYPH
cord-009318-zt1o1bcz	558	137	molecule	molecule	NN
cord-009318-zt1o1bcz	558	138	enzyme	enzyme	NN
cord-009318-zt1o1bcz	558	139	-	-	HYPH
cord-009318-zt1o1bcz	558	140	linked	link	VBN
cord-009318-zt1o1bcz	558	141	immunosorbent	immunosorbent	JJ
cord-009318-zt1o1bcz	558	142	assay	assay	NN
cord-009318-zt1o1bcz	558	143	detects	detect	VBZ
cord-009318-zt1o1bcz	558	144	serum	serum	NN
cord-009318-zt1o1bcz	558	145	proteins	protein	NNS
cord-009318-zt1o1bcz	558	146	at	at	IN
cord-009318-zt1o1bcz	558	147	subfemtomolar	subfemtomolar	JJ
cord-009318-zt1o1bcz	558	148	concentrations	concentration	NNS
cord-009318-zt1o1bcz	558	149	Digital	Digital	NNP
cord-009318-zt1o1bcz	558	150	concentration	concentration	NN
cord-009318-zt1o1bcz	558	151	readout	readout	NN
cord-009318-zt1o1bcz	558	152	of	of	IN
cord-009318-zt1o1bcz	558	153	single	single	JJ
cord-009318-zt1o1bcz	558	154	enzyme	enzyme	NN
cord-009318-zt1o1bcz	558	155	molecules	molecule	NNS
cord-009318-zt1o1bcz	558	156	using	use	VBG
cord-009318-zt1o1bcz	558	157	femtoliter	femtoliter	NNP
cord-009318-zt1o1bcz	558	158	arrays	array	NNS
cord-009318-zt1o1bcz	558	159	and	and	CC
cord-009318-zt1o1bcz	558	160	poisson	poisson	NNP
cord-009318-zt1o1bcz	558	161	statistics	statistic	NNS
cord-009318-zt1o1bcz	558	162	Integrated	integrate	VBN
cord-009318-zt1o1bcz	558	163	bacterial	bacterial	JJ
cord-009318-zt1o1bcz	558	164	identification	identification	NN
cord-009318-zt1o1bcz	558	165	and	and	CC
cord-009318-zt1o1bcz	558	166	antimicrobial	antimicrobial	JJ
cord-009318-zt1o1bcz	558	167	susceptibility	susceptibility	NN
cord-009318-zt1o1bcz	558	168	testing	testing	NN
cord-009318-zt1o1bcz	558	169	using	use	VBG
cord-009318-zt1o1bcz	558	170	PCR	PCR	NNP
cord-009318-zt1o1bcz	558	171	and	and	CC
cord-009318-zt1o1bcz	558	172	high	high	JJ
cord-009318-zt1o1bcz	558	173	-	-	HYPH
cord-009318-zt1o1bcz	558	174	resolution	resolution	NN
cord-009318-zt1o1bcz	558	175	melt	melt	NN
cord-009318-zt1o1bcz	558	176	Massively	massively	RB
cord-009318-zt1o1bcz	558	177	parallel	parallel	JJ
cord-009318-zt1o1bcz	558	178	digital	digital	JJ
cord-009318-zt1o1bcz	558	179	high	high	JJ
cord-009318-zt1o1bcz	558	180	resolution	resolution	NN
cord-009318-zt1o1bcz	558	181	melt	melt	NN
cord-009318-zt1o1bcz	558	182	for	for	IN
cord-009318-zt1o1bcz	558	183	rapid	rapid	JJ
cord-009318-zt1o1bcz	558	184	and	and	CC
cord-009318-zt1o1bcz	558	185	absolutely	absolutely	RB
cord-009318-zt1o1bcz	558	186	quantitative	quantitative	JJ
cord-009318-zt1o1bcz	558	187	sequence	sequence	NN
cord-009318-zt1o1bcz	558	188	profiling	profile	VBG
cord-009318-zt1o1bcz	558	189	Facile	facile	JJ
cord-009318-zt1o1bcz	558	190	profiling	profiling	NN
cord-009318-zt1o1bcz	558	191	of	of	IN
cord-009318-zt1o1bcz	558	192	molecular	molecular	JJ
cord-009318-zt1o1bcz	558	193	heterogeneity	heterogeneity	NN
cord-009318-zt1o1bcz	558	194	by	by	IN
cord-009318-zt1o1bcz	558	195	microfluidic	microfluidic	NN
cord-009318-zt1o1bcz	559	1	digital	digital	NNP
cord-009318-zt1o1bcz	559	2	melt	melt	NNP
cord-009318-zt1o1bcz	560	1	Nanoarray	nanoarray	JJ
cord-009318-zt1o1bcz	560	2	digital	digital	JJ
cord-009318-zt1o1bcz	560	3	polymerase	polymerase	NN
cord-009318-zt1o1bcz	560	4	chain	chain	NN
cord-009318-zt1o1bcz	560	5	reaction	reaction	NN
cord-009318-zt1o1bcz	560	6	with	with	IN
cord-009318-zt1o1bcz	560	7	high	high	JJ
cord-009318-zt1o1bcz	560	8	-	-	HYPH
cord-009318-zt1o1bcz	560	9	resolution	resolution	NN
cord-009318-zt1o1bcz	560	10	melt	melt	NN
cord-009318-zt1o1bcz	560	11	for	for	IN
cord-009318-zt1o1bcz	560	12	enabling	enable	VBG
cord-009318-zt1o1bcz	560	13	broad	broad	JJ
cord-009318-zt1o1bcz	560	14	bacteria	bacteria	NNS
cord-009318-zt1o1bcz	560	15	identification	identification	NN
cord-009318-zt1o1bcz	560	16	and	and	CC
cord-009318-zt1o1bcz	560	17	pheno	pheno	NN
cord-009318-zt1o1bcz	560	18	-	-	HYPH
cord-009318-zt1o1bcz	560	19	molecular	molecular	JJ
cord-009318-zt1o1bcz	560	20	antimicrobial	antimicrobial	JJ
cord-009318-zt1o1bcz	560	21	susceptibility	susceptibility	NN
cord-009318-zt1o1bcz	560	22	test	test	NN
cord-009318-zt1o1bcz	560	23	Highly	highly	RB
cord-009318-zt1o1bcz	560	24	efficient	efficient	JJ
cord-009318-zt1o1bcz	560	25	real	real	JJ
cord-009318-zt1o1bcz	560	26	-	-	HYPH
cord-009318-zt1o1bcz	560	27	time	time	NN
cord-009318-zt1o1bcz	560	28	droplet	droplet	NN
cord-009318-zt1o1bcz	560	29	analysis	analysis	NN
cord-009318-zt1o1bcz	560	30	platform	platform	NN
cord-009318-zt1o1bcz	560	31	for	for	IN
cord-009318-zt1o1bcz	560	32	high	high	JJ
cord-009318-zt1o1bcz	560	33	-	-	HYPH
cord-009318-zt1o1bcz	560	34	throughput	throughput	NN
cord-009318-zt1o1bcz	560	35	interrogation	interrogation	NN
cord-009318-zt1o1bcz	560	36	of	of	IN
cord-009318-zt1o1bcz	560	37	DNA	dna	NN
cord-009318-zt1o1bcz	560	38	sequences	sequence	NNS
cord-009318-zt1o1bcz	560	39	by	by	IN
cord-009318-zt1o1bcz	560	40	melt	melt	NN
cord-009318-zt1o1bcz	561	1	This	this	DT
cord-009318-zt1o1bcz	561	2	project	project	NN
cord-009318-zt1o1bcz	561	3	benefited	benefit	VBD
cord-009318-zt1o1bcz	561	4	from	from	IN
cord-009318-zt1o1bcz	561	5	the	the	DT
cord-009318-zt1o1bcz	561	6	use	use	NN
cord-009318-zt1o1bcz	561	7	of	of	IN
cord-009318-zt1o1bcz	561	8	instrumentation	instrumentation	NN
cord-009318-zt1o1bcz	561	9	at	at	IN
cord-009318-zt1o1bcz	561	10	the	the	DT
cord-009318-zt1o1bcz	561	11	Jim	Jim	NNP
cord-009318-zt1o1bcz	561	12	Hall	Hall	NNP
cord-009318-zt1o1bcz	561	13	Design	Design	NNP
cord-009318-zt1o1bcz	561	14	and	and	CC
cord-009318-zt1o1bcz	561	15	Prototyping	Prototyping	NNP
cord-009318-zt1o1bcz	561	16	Lab	Lab	NNP
cord-009318-zt1o1bcz	561	17	and	and	CC
cord-009318-zt1o1bcz	561	18	the	the	DT
cord-009318-zt1o1bcz	561	19	Millard	Millard	NNP
cord-009318-zt1o1bcz	561	20	e42	e42	CD
cord-009318-zt1o1bcz	561	21	Nucleic	Nucleic	NNP
cord-009318-zt1o1bcz	561	22	Acids	Acids	NNPS
cord-009318-zt1o1bcz	561	23	Research	Research	NNP
cord-009318-zt1o1bcz	561	24	,	,	,
cord-009318-zt1o1bcz	561	25	2020	2020	CD
cord-009318-zt1o1bcz	561	26	,	,	,
cord-009318-zt1o1bcz	561	27	Vol	Vol	NNP
cord-009318-zt1o1bcz	561	28	.	.	.
cord-009318-zt1o1bcz	562	1	48	48	CD
cord-009318-zt1o1bcz	562	2	,	,	,
cord-009318-zt1o1bcz	562	3	No	no	NN
cord-009318-zt1o1bcz	562	4	.	.	NN
cord-009318-zt1o1bcz	562	5	7	7	CD
cord-009318-zt1o1bcz	562	6	PAGE	page	NN
cord-009318-zt1o1bcz	562	7	20	20	CD
cord-009318-zt1o1bcz	562	8	OF	of	IN
cord-009318-zt1o1bcz	562	9	21	21	CD
cord-009318-zt1o1bcz	562	10	and	and	CC
cord-009318-zt1o1bcz	562	11	Muriel	Muriel	NNP
cord-009318-zt1o1bcz	562	12	Jacobs	Jacobs	NNP
cord-009318-zt1o1bcz	562	13	Genetics	Genetics	NNP
cord-009318-zt1o1bcz	562	14	and	and	CC
cord-009318-zt1o1bcz	562	15	Genomics	Genomics	NNPS
cord-009318-zt1o1bcz	562	16	Laboratory	Laboratory	NNP
cord-009318-zt1o1bcz	562	17	.	.	.
cord-009318-zt1o1bcz	563	1	We	-PRON-	PRP
cord-009318-zt1o1bcz	563	2	thank	thank	VBP
cord-009318-zt1o1bcz	563	3	Daan	Daan	NNP
cord-009318-zt1o1bcz	563	4	Witters	Witters	NNP
cord-009318-zt1o1bcz	563	5	and	and	CC
cord-009318-zt1o1bcz	563	6	Pedro	Pedro	NNP
cord-009318-zt1o1bcz	563	7	Ojeda	Ojeda	NNP
cord-009318-zt1o1bcz	563	8	for	for	IN
cord-009318-zt1o1bcz	563	9	initial	initial	JJ
cord-009318-zt1o1bcz	563	10	selection	selection	NN
cord-009318-zt1o1bcz	563	11	of	of	IN
cord-009318-zt1o1bcz	563	12	CT	CT	NNP
cord-009318-zt1o1bcz	563	13	primers	primer	NNS
cord-009318-zt1o1bcz	563	14	,	,	,
cord-009318-zt1o1bcz	563	15	Eric	Eric	NNP
cord-009318-zt1o1bcz	563	16	Liaw	Liaw	NNP
cord-009318-zt1o1bcz	563	17	for	for	IN
cord-009318-zt1o1bcz	563	18	helpful	helpful	JJ
cord-009318-zt1o1bcz	563	19	discussions	discussion	NNS
cord-009318-zt1o1bcz	563	20	of	of	IN
cord-009318-zt1o1bcz	563	21	error	error	NN
cord-009318-zt1o1bcz	563	22	propagation	propagation	NN
cord-009318-zt1o1bcz	563	23	and	and	CC
cord-009318-zt1o1bcz	563	24	Natasha	Natasha	NNP
cord-009318-zt1o1bcz	563	25	Shelby	Shelby	NNP
cord-009318-zt1o1bcz	563	26	for	for	IN
cord-009318-zt1o1bcz	563	27	help	help	NN
cord-009318-zt1o1bcz	563	28	with	with	IN
cord-009318-zt1o1bcz	563	29	writing	writing	NN
cord-009318-zt1o1bcz	563	30	and	and	CC
cord-009318-zt1o1bcz	563	31	editing	edit	VBG
cord-009318-zt1o1bcz	563	32	this	this	DT
cord-009318-zt1o1bcz	563	33	manuscript	manuscript	NN
cord-009318-zt1o1bcz	563	34	.	.	.
cord-009318-zt1o1bcz	564	1	Conflict	conflict	NN
cord-009318-zt1o1bcz	564	2	of	of	IN
cord-009318-zt1o1bcz	564	3	interest	interest	NN
cord-009318-zt1o1bcz	564	4	statement	statement	NN
cord-009318-zt1o1bcz	564	5	.	.	.
cord-009318-zt1o1bcz	565	1	The	the	DT
cord-009318-zt1o1bcz	565	2	content	content	NN
cord-009318-zt1o1bcz	565	3	of	of	IN
cord-009318-zt1o1bcz	565	4	this	this	DT
cord-009318-zt1o1bcz	565	5	manuscript	manuscript	NN
cord-009318-zt1o1bcz	565	6	is	be	VBZ
cord-009318-zt1o1bcz	565	7	the	the	DT
cord-009318-zt1o1bcz	565	8	subject	subject	NN
cord-009318-zt1o1bcz	565	9	of	of	IN
cord-009318-zt1o1bcz	565	10	a	a	DT
cord-009318-zt1o1bcz	565	11	patent	patent	NN
cord-009318-zt1o1bcz	565	12	application	application	NN
cord-009318-zt1o1bcz	565	13	filed	file	VBN
cord-009318-zt1o1bcz	565	14	by	by	IN
cord-009318-zt1o1bcz	565	15	Caltech	Caltech	NNP
cord-009318-zt1o1bcz	565	16	.	.	.
cord-009318-zt1o1bcz	566	1	Supplementary	Supplementary	NNP
cord-009318-zt1o1bcz	566	2	Data	Data	NNP
cord-009318-zt1o1bcz	566	3	are	be	VBP
cord-009318-zt1o1bcz	566	4	available	available	JJ
cord-009318-zt1o1bcz	566	5	at	at	IN
cord-009318-zt1o1bcz	566	6	NAR	NAR	NNP
cord-009318-zt1o1bcz	566	7	Online	Online	NNP
cord-009318-zt1o1bcz	566	8	.	.	.