id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-350189-2su7oqbz	Elmén, Joacim	Locked nucleic acid (LNA) mediated improvements in siRNA stability and functionality	2005-01-14		.txt	text/plain	5415	322	55	A priori, this suggests that LNA may be used to increase the functional half-life of siRNA in vivo by two different mechanisms, e.g. by enhancing the resistance of the constituent RNA strands against degradation by single-stranded RNases and by stabilizing the siRNA duplex structure that is critical for activity. Next, we examined the effect of making single RNA to LNA exchanges at base-paired positions in the antisense strand of the firefly luciferase siLNA1. Although we cannot exclude that these modifications somehow prevent loading of the antisense strand into RISC, we believe this to be unlikely given the functionality of many significantly more modified siLNAs. Rather, as these positions are all close to the site where RNA target cleavage occurs [between pos. The SARS siRNA (Table 1) has identical closing base-pairs at both ends (A:U) making it likely that enough of both the antisense and sense strand would be incorporated into RISC to observe activity on the respective targets.	./cache/cord-350189-2su7oqbz.txt	./txt/cord-350189-2su7oqbz.txt