id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-320627-7vi6skvh	Horejsh, Douglas	A molecular beacon, bead-based assay for the detection of nucleic acids by flow cytometry	2005-01-19		.txt	text/plain	3725	172	48	We have developed a fluid array system using microsphere-conjugated molecular beacons and the flow cytometer for the specific, multiplexed detection of unlabelled nucleic acids in solution. Using beads of different sizes and molecular beacons in two fluorophore colours, synthetic nucleic acid control sequences were specifically detected for three respiratory pathogens, including the SARS coronavirus in proof-of-concept experiments. In this report, we describe the construction of molecular beacon-conjugated beads that we have called 'BeadCons', whose specific hybridization with complementary target sequences can be resolved by flow cytometry (see Figure 1 ). In the multiplex detection experiment, the test sample contained 0.5 ml of the positive oligo DNA (100 mM stock) diluted in 9.5 ml of a complex mixture of oligonucleotides (equimolar levels of 10 mM each, equalling a 100 mM total concentration; sequences listed in Supplementary Table 1 ).	./cache/cord-320627-7vi6skvh.txt	./txt/cord-320627-7vi6skvh.txt