id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002494-qm9urt2w	Blank, Maximilian F.	SIRT7-dependent deacetylation of CDK9 activates RNA polymerase II transcription	2017-03-17		.txt	text/plain	6796	407	52	In addition to its role in Pol I transcription, we found that SIRT7 also regulates transcription of snoRNAs and mRNAs. Mechanistically, SIRT7 promotes the release of P-TEFb from the inactive 7SK snRNP complex and deacetylates CDK9, a subunit of the elongation factor P-TEFb, which activates transcription by phosphorylating serine 2 within the C-terminal domain (CTD) of Pol II. To investigate whether this region is important for RNA-dependent protein interactions, 5external spacer (5 -ETS) RNA was immobilized on streptavidin beads and incubated with lysates of cells expressing Flag-tagged wildtype SIRT7 or mutants lacking 32 ( N32) or 78 N-terminal amino acids ( N78). To test whether SIRT7 affects Pol II-dependent transcription of snoRNAs, we overexpressed Flag-SIRT7 in HEK293T cells and monitored RNA levels by RT-qPCR. Together with the observation that SIRT7 interacts with elongating Pol II and co-localizes with Pol II phosphorylated at CTD-Ser2 at SIRT7 target genes, these results suggested that reversible acetylation may regulate CDK9 kinase activity and transcription elongation.	./cache/cord-002494-qm9urt2w.txt	./txt/cord-002494-qm9urt2w.txt