id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000532-e18licyc	Tholstrup, Jesper	mRNA pseudoknot structures can act as ribosomal roadblocks	2011-09-08		.txt	text/plain	5809	270	57	The results shown in Figure 2 revealed that a 1D SDS-PAGE assay could not firmly identify polypeptides originating from a À1 frameshifted ribosome stalled in the pseudoknot from the non-frameshifted product in a 'Downstream Stop' construct. In order to quantify the amount of À1 frameshifted ribosomes stalled inside the pseudoknot, we performed a 2D SDS-PAGE separation of the radioactively labelled proteins originating from the 'Upstream Stop' construct (Supplementary Figures S4 and S5) , which is the type of construct most commonly used throughout literature. In the 'Upstream Stop' construct the non-frameshifting ribosomes will translate gene10 and terminate at a UAA stop codon in the spacer sequence and produce a 28 kDa polypeptide. In the following subsections 'Identification of transcripts from the T7gene10-PK-lacZ gene fusions', 'Messenger RNA stability' and 'Coupling between translation and transcription is required for full-length transcripts', we will show that the observed proteins did indeed originate from stalled ribosomes and that they were not caused by other effects.	./cache/cord-000532-e18licyc.txt	./txt/cord-000532-e18licyc.txt