id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000271-812uc4w7	Chen, Zhiqi	Alternative splicing of CD200 is regulated by an exonic splicing enhancer and SF2/ASF	2010-06-17		.txt	text/plain	6950	352	57	Taken together, our data suggest for the first time that SF2/ASF regulates the function of CD200 by controlling CD200 alternative splicing, through direct binding to an ESE located in exon 2 of CD200. Interestingly, in a mouse model of viral infection, we detected for the first time that the normal splicing pattern of CD200 was reversed in the lung tissue of A/J mice infected with mouse hepatitis virus strain I (MHV-1), following an increase in expression of SF2/ASF in this MHV-1 susceptible mouse strain. Two-and-a-half micrograms of siRNA, together with 10 mg of the alternative splicing construct DNA, was transfected to Daudi or SK-N cells by electroporation to detect exogenous expression pattern of CD200 following silencing SF2/ASF. As shown in Figures 3C and D, and 4A and B, expression of the full-length transcript (exon 2 inclusion) was reduced in both Daudi and SK-N cells after mutation or deletion of the ESE in exon 2.	./cache/cord-000271-812uc4w7.txt	./txt/cord-000271-812uc4w7.txt