id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003341-z5w56zeq	Nguyen, Thi Thanh Hanh	In Vitro Evaluation of Novel Inhibitors against the NS2B-NS3 Protease of Dengue Fever Virus Type 4	2013-12-13		.txt	text/plain	3407	193	55	Virtual screening of 300,000 compounds using Autodock 3 on the GVSS platform was conducted to identify novel inhibitors against the NS2B-NS3(pro). pastoris DENV4 NS2B-NS3 pro , fused to the α-factor secretion signal sequence and placed under the control of the methanol inducible alcohol oxidase promoter, was constructed to express the secreted protein in P. Thirty-six compounds were selected for in vitro assay based on their free energy binding (Table S1 ) and H-bonds interactions with amino acid residues at the NS3 pro active site. Among 300,000 compounds, 36 were selected for testing in vitro inhibitory activity against NS2B-NS3 pro based on hydrogen bond interactions [25, 36] . Activity of recombinant dengue 2 virus NS3 protease in the presence of a truncated NS2B co-factor, small peptide substrates, and inhibitors Novel dengue virus-specific NS2B/NS3 protease inhibitor, BP2109, discovered by a high-throughput screening assay	./cache/cord-003341-z5w56zeq.txt	./txt/cord-003341-z5w56zeq.txt