id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002026-wybmer7o	Chung, Dong Hoon	HTS-Driven Discovery of New Chemotypes with West Nile Virus Inhibitory Activity	2010-03-12		.txt	text/plain	3789	209	61	We developed a high throughput screen to discover small molecule probes that inhibit virus infection of Vero E6 cells. Twenty-six compounds showed a dose response in the HT screen and were further evaluated in a time of addition assay and in a titer reduction assay. To confirm the activity and to test the cytotoxicity, the top 110 inhibitory compounds were evaluated in a dose response assay using the identical screen. All seven compounds screened in the time of addition experiment were confirmed to have anti-WNV activity based on the titer reduction assay and EC 90 values between 2.14~26.16 µM. None of the compounds showed anti-WNV activity as good as the positive control drug, MPA, however, the mechanism of MPA is not specific to the virus making the probes we identified of significant interest. The serially diluted compounds were also used for time of addition studies adding to the infected cells in 384-well plates at -1, 4, 8 and18 h post-infection with WNV (0.02 MOI).	./cache/cord-002026-wybmer7o.txt	./txt/cord-002026-wybmer7o.txt