id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-273391-vmtfn78x	Li, Kun	Single-Dose, Intranasal Immunization with Recombinant Parainfluenza Virus 5 Expressing Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike Protein Protects Mice from Fatal MERS-CoV Infection	2020-04-07		.txt	text/plain	5258	291	53	title: Single-Dose, Intranasal Immunization with Recombinant Parainfluenza Virus 5 Expressing Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike Protein Protects Mice from Fatal MERS-CoV Infection In this study, we evaluated parainfluenza virus 5 (PIV5)-based vaccine expressing the MERS-CoV envelope spike protein (PIV5/MERS-S) in a human DPP4 knockin C57BL/6 congenic mouse model (hDPP4 KI). Following a single-dose intranasal immunization, PIV5-MERS-S induced neutralizing antibody and robust T cell responses in hDPP4 KI mice. As shown in Fig. 3B to D, we observed a significant increase in the Immunization with PIV5 Expressing MERS-CoV Spike ® percentage and total number of CD8 ϩ -IFN-␥ ϩ cells in the lungs of PIV5-MERS-Simmunized mice in comparison to those infected with PIV5-GFP virus, consistent with a MERS-S-specific primary CD8 T cell response in the lungs. The finding that PIV5 expressing MERS S protected mice against lethal MERS-CoV challenge at a single low dose of 10 4 PFU suggests its potential use as a vaccine vector for emerging viruses such as SARS-CoV-2.	./cache/cord-273391-vmtfn78x.txt	./txt/cord-273391-vmtfn78x.txt