id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001223-6sb3ipab	Lennemann, Nicholas J.	Comprehensive Functional Analysis of N-Linked Glycans on Ebola Virus GP1	2014-01-28		.txt	text/plain	5783	282	54	In total, our results provide evidence that the conserved N-linked glycans on the EBOV GP1 core protect GP from antibody neutralization despite the negative impact the glycans have on viral entry efficiency. Since complete deglycosylation of the core domains of GP1 did not negatively impact the expression of GP or the transduction of pseudotyped virions, we systematically combined N-linked glycan mutations in the MLD with our 7G mutant. Pseudovirion entry mediated by GP1⌬muc, GP, the 6G mutant, containing a fully deglycosylated glycan cap, and the GPm8G mutant containing a MLD that was fully deglycosylated for N-linked glycans were abrogated by treating Vero cells with the CatB inhibitor CA-074, which profoundly blocked CatB activity (Fig. S4A ). Our findings that deglycosylation of GP pseudovirions enhances the transduction of Vero cells and peritoneal macrophages provides evidence that the N-glycans on GP1 decrease the efficiency of the entry process.	./cache/cord-001223-6sb3ipab.txt	./txt/cord-001223-6sb3ipab.txt