id	sid	tid	token	lemma	pos
cord-297160-tqw9vx2b	1	1	key	key	JJ
cord-297160-tqw9vx2b	1	2	:	:	:
cord-297160-tqw9vx2b	1	3	cord-297160-tqw9vx2b	cord-297160-tqw9vx2b	NN
cord-297160-tqw9vx2b	1	4	authors	author	NNS
cord-297160-tqw9vx2b	1	5	:	:	:
cord-297160-tqw9vx2b	1	6	Geerligs	Geerligs	NNP
cord-297160-tqw9vx2b	1	7	,	,	,
cord-297160-tqw9vx2b	1	8	H.J.	H.J.	NNP
cord-297160-tqw9vx2b	1	9	;	;	:
cord-297160-tqw9vx2b	1	10	Meinders	Meinders	NNP
cord-297160-tqw9vx2b	1	11	,	,	,
cord-297160-tqw9vx2b	1	12	C.A.M.	C.A.M.	NNP
cord-297160-tqw9vx2b	1	13	;	;	:
cord-297160-tqw9vx2b	1	14	Snel	Snel	NNP
cord-297160-tqw9vx2b	1	15	,	,	,
cord-297160-tqw9vx2b	1	16	J.	J.	NNP
cord-297160-tqw9vx2b	1	17	;	;	:
cord-297160-tqw9vx2b	1	18	Duyves	Duyves	NNP
cord-297160-tqw9vx2b	1	19	,	,	,
cord-297160-tqw9vx2b	1	20	W.	W.	NNP
cord-297160-tqw9vx2b	1	21	title	title	NN
cord-297160-tqw9vx2b	1	22	:	:	:
cord-297160-tqw9vx2b	1	23	The	the	DT
cord-297160-tqw9vx2b	1	24	use	use	NN
cord-297160-tqw9vx2b	1	25	of	of	IN
cord-297160-tqw9vx2b	1	26	RT	RT	NNP
cord-297160-tqw9vx2b	1	27	-	-	HYPH
cord-297160-tqw9vx2b	1	28	PCR	PCR	NNP
cord-297160-tqw9vx2b	1	29	for	for	IN
cord-297160-tqw9vx2b	1	30	determination	determination	NN
cord-297160-tqw9vx2b	1	31	of	of	IN
cord-297160-tqw9vx2b	1	32	separate	separate	JJ
cord-297160-tqw9vx2b	1	33	end	end	NN
cord-297160-tqw9vx2b	1	34	-	-	HYPH
cord-297160-tqw9vx2b	1	35	points	point	NNS
cord-297160-tqw9vx2b	1	36	for	for	IN
cord-297160-tqw9vx2b	1	37	the	the	DT
cord-297160-tqw9vx2b	1	38	strains	strain	NNS
cord-297160-tqw9vx2b	1	39	IB	IB	NNP
cord-297160-tqw9vx2b	1	40	H120	H120	NNP
cord-297160-tqw9vx2b	1	41	and	and	CC
cord-297160-tqw9vx2b	1	42	IB	IB	NNP
cord-297160-tqw9vx2b	1	43	D274	D274	NNP
cord-297160-tqw9vx2b	1	44	in	in	IN
cord-297160-tqw9vx2b	1	45	titration	titration	NN
cord-297160-tqw9vx2b	1	46	of	of	IN
cord-297160-tqw9vx2b	1	47	the	the	DT
cord-297160-tqw9vx2b	1	48	combination	combination	NN
cord-297160-tqw9vx2b	1	49	vaccine	vaccine	NN
cord-297160-tqw9vx2b	1	50	Poulvac	Poulvac	NNP
cord-297160-tqw9vx2b	1	51	IB	IB	NNP
cord-297160-tqw9vx2b	1	52	(	(	-LRB-
cord-297160-tqw9vx2b	2	1	®	®	LS
cord-297160-tqw9vx2b	2	2	)	)	-RRB-
cord-297160-tqw9vx2b	2	3	primer	primer	NN
cord-297160-tqw9vx2b	2	4	date	date	NN
cord-297160-tqw9vx2b	2	5	:	:	:
cord-297160-tqw9vx2b	2	6	2013	2013	CD
cord-297160-tqw9vx2b	2	7	-	-	SYM
cord-297160-tqw9vx2b	2	8	07	07	CD
cord-297160-tqw9vx2b	2	9	-	-	HYPH
cord-297160-tqw9vx2b	2	10	01	01	CD
cord-297160-tqw9vx2b	2	11	journal	journal	NN
cord-297160-tqw9vx2b	2	12	:	:	:
cord-297160-tqw9vx2b	3	1	J	J	NNP
cord-297160-tqw9vx2b	3	2	Virol	Virol	NNP
cord-297160-tqw9vx2b	3	3	Methods	Methods	NNPS
cord-297160-tqw9vx2b	4	1	DOI	DOI	NNP
cord-297160-tqw9vx2b	4	2	:	:	:
cord-297160-tqw9vx2b	4	3	10.1016/	10.1016/	CD
cord-297160-tqw9vx2b	5	1	j.jviromet.2013.06.029	j.jviromet.2013.06.029	NNP
cord-297160-tqw9vx2b	6	1	sha	sha	NNP
cord-297160-tqw9vx2b	6	2	:	:	:
cord-297160-tqw9vx2b	6	3	2ca4dea2e06fcb52b003a52eb3bd502c964e3da5	2ca4dea2e06fcb52b003a52eb3bd502c964e3da5	CD
cord-297160-tqw9vx2b	6	4	doc_id	doc_id	CD
cord-297160-tqw9vx2b	6	5	:	:	:
cord-297160-tqw9vx2b	6	6	297160	297160	CD
cord-297160-tqw9vx2b	6	7	cord_uid	cord_uid	NNS
cord-297160-tqw9vx2b	6	8	:	:	:
cord-297160-tqw9vx2b	6	9	tqw9vx2b	tqw9vx2b	CD
cord-297160-tqw9vx2b	6	10	Poulvac	Poulvac	NNP
cord-297160-tqw9vx2b	6	11	IB	IB	NNP
cord-297160-tqw9vx2b	6	12	(	(	-LRB-
cord-297160-tqw9vx2b	6	13	®	®	NN
cord-297160-tqw9vx2b	6	14	)	)	-RRB-
cord-297160-tqw9vx2b	7	1	Primer	primer	NN
cord-297160-tqw9vx2b	7	2	is	be	VBZ
cord-297160-tqw9vx2b	7	3	a	a	DT
cord-297160-tqw9vx2b	7	4	lyophilized	lyophilized	JJ
cord-297160-tqw9vx2b	7	5	vaccine	vaccine	NN
cord-297160-tqw9vx2b	7	6	containing	contain	VBG
cord-297160-tqw9vx2b	7	7	two	two	CD
cord-297160-tqw9vx2b	7	8	attenuated	attenuated	JJ
cord-297160-tqw9vx2b	7	9	infectious	infectious	JJ
cord-297160-tqw9vx2b	7	10	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	7	11	strains	strain	NNS
cord-297160-tqw9vx2b	7	12	in	in	IN
cord-297160-tqw9vx2b	7	13	one	one	CD
cord-297160-tqw9vx2b	7	14	vial	vial	NN
cord-297160-tqw9vx2b	7	15	,	,	,
cord-297160-tqw9vx2b	7	16	IB	IB	NNP
cord-297160-tqw9vx2b	7	17	H120	H120	NNP
cord-297160-tqw9vx2b	7	18	and	and	CC
cord-297160-tqw9vx2b	7	19	IB	IB	NNP
cord-297160-tqw9vx2b	7	20	D274	D274	NNP
cord-297160-tqw9vx2b	7	21	.	.	.
cord-297160-tqw9vx2b	8	1	For	for	IN
cord-297160-tqw9vx2b	8	2	quantification	quantification	NN
cord-297160-tqw9vx2b	8	3	of	of	IN
cord-297160-tqw9vx2b	8	4	the	the	DT
cord-297160-tqw9vx2b	8	5	viral	viral	JJ
cord-297160-tqw9vx2b	8	6	content	content	NN
cord-297160-tqw9vx2b	8	7	of	of	IN
cord-297160-tqw9vx2b	8	8	the	the	DT
cord-297160-tqw9vx2b	8	9	vaccine	vaccine	NN
cord-297160-tqw9vx2b	8	10	,	,	,
cord-297160-tqw9vx2b	8	11	dilution	dilution	NN
cord-297160-tqw9vx2b	8	12	series	series	NN
cord-297160-tqw9vx2b	8	13	of	of	IN
cord-297160-tqw9vx2b	8	14	the	the	DT
cord-297160-tqw9vx2b	8	15	final	final	JJ
cord-297160-tqw9vx2b	8	16	product	product	NN
cord-297160-tqw9vx2b	8	17	are	be	VBP
cord-297160-tqw9vx2b	8	18	inoculated	inoculate	VBN
cord-297160-tqw9vx2b	8	19	in	in	IN
cord-297160-tqw9vx2b	8	20	embryonated	embryonate	VBN
cord-297160-tqw9vx2b	8	21	chicken	chicken	NN
cord-297160-tqw9vx2b	8	22	eggs	egg	NNS
cord-297160-tqw9vx2b	8	23	.	.	.
cord-297160-tqw9vx2b	9	1	After	after	IN
cord-297160-tqw9vx2b	9	2	the	the	DT
cord-297160-tqw9vx2b	9	3	incubation	incubation	NN
cord-297160-tqw9vx2b	9	4	period	period	NN
cord-297160-tqw9vx2b	9	5	of	of	IN
cord-297160-tqw9vx2b	9	6	seven	seven	CD
cord-297160-tqw9vx2b	9	7	days	day	NNS
cord-297160-tqw9vx2b	9	8	standard	standard	JJ
cord-297160-tqw9vx2b	9	9	practice	practice	NN
cord-297160-tqw9vx2b	9	10	is	be	VBZ
cord-297160-tqw9vx2b	9	11	for	for	IN
cord-297160-tqw9vx2b	9	12	the	the	DT
cord-297160-tqw9vx2b	9	13	embryos	embryo	NNS
cord-297160-tqw9vx2b	9	14	to	to	TO
cord-297160-tqw9vx2b	9	15	be	be	VB
cord-297160-tqw9vx2b	9	16	taken	take	VBN
cord-297160-tqw9vx2b	9	17	from	from	IN
cord-297160-tqw9vx2b	9	18	each	each	DT
cord-297160-tqw9vx2b	9	19	egg	egg	NN
cord-297160-tqw9vx2b	9	20	and	and	CC
cord-297160-tqw9vx2b	9	21	examined	examine	VBD
cord-297160-tqw9vx2b	9	22	visually	visually	RB
cord-297160-tqw9vx2b	9	23	for	for	IN
cord-297160-tqw9vx2b	9	24	IB	IB	NNP
cord-297160-tqw9vx2b	9	25	specific	specific	JJ
cord-297160-tqw9vx2b	9	26	lesions	lesion	NNS
cord-297160-tqw9vx2b	9	27	;	;	:
cord-297160-tqw9vx2b	9	28	these	these	DT
cord-297160-tqw9vx2b	9	29	readings	reading	NNS
cord-297160-tqw9vx2b	9	30	are	be	VBP
cord-297160-tqw9vx2b	9	31	used	use	VBN
cord-297160-tqw9vx2b	9	32	to	to	TO
cord-297160-tqw9vx2b	9	33	determine	determine	VB
cord-297160-tqw9vx2b	9	34	an	an	DT
cord-297160-tqw9vx2b	9	35	end	end	NN
cord-297160-tqw9vx2b	9	36	-	-	HYPH
cord-297160-tqw9vx2b	9	37	point	point	NN
cord-297160-tqw9vx2b	9	38	in	in	IN
cord-297160-tqw9vx2b	9	39	viral	viral	JJ
cord-297160-tqw9vx2b	9	40	titrations	titration	NNS
cord-297160-tqw9vx2b	9	41	.	.	.
cord-297160-tqw9vx2b	10	1	The	the	DT
cord-297160-tqw9vx2b	10	2	result	result	NN
cord-297160-tqw9vx2b	10	3	is	be	VBZ
cord-297160-tqw9vx2b	10	4	a	a	DT
cord-297160-tqw9vx2b	10	5	titre	titre	NN
cord-297160-tqw9vx2b	10	6	value	value	NN
cord-297160-tqw9vx2b	10	7	to	to	TO
cord-297160-tqw9vx2b	10	8	which	which	WDT
cord-297160-tqw9vx2b	10	9	both	both	DT
cord-297160-tqw9vx2b	10	10	strains	strain	NNS
cord-297160-tqw9vx2b	10	11	contribute	contribute	VBP
cord-297160-tqw9vx2b	10	12	.	.	.
cord-297160-tqw9vx2b	11	1	However	however	RB
cord-297160-tqw9vx2b	11	2	,	,	,
cord-297160-tqw9vx2b	11	3	it	-PRON-	PRP
cord-297160-tqw9vx2b	11	4	is	be	VBZ
cord-297160-tqw9vx2b	11	5	not	not	RB
cord-297160-tqw9vx2b	11	6	clear	clear	JJ
cord-297160-tqw9vx2b	11	7	what	what	WP
cord-297160-tqw9vx2b	11	8	the	the	DT
cord-297160-tqw9vx2b	11	9	live	live	JJ
cord-297160-tqw9vx2b	11	10	virus	virus	NN
cord-297160-tqw9vx2b	11	11	titre	titre	NN
cord-297160-tqw9vx2b	11	12	is	be	VBZ
cord-297160-tqw9vx2b	11	13	for	for	IN
cord-297160-tqw9vx2b	11	14	strain	strain	NN
cord-297160-tqw9vx2b	11	15	IB	IB	NNP
cord-297160-tqw9vx2b	11	16	H120	H120	NNP
cord-297160-tqw9vx2b	11	17	and	and	CC
cord-297160-tqw9vx2b	11	18	for	for	IN
cord-297160-tqw9vx2b	11	19	strain	strain	NN
cord-297160-tqw9vx2b	11	20	IB	IB	NNP
cord-297160-tqw9vx2b	11	21	D274	D274	NNP
cord-297160-tqw9vx2b	11	22	.	.	.
cord-297160-tqw9vx2b	12	1	In	in	IN
cord-297160-tqw9vx2b	12	2	order	order	NN
cord-297160-tqw9vx2b	12	3	to	to	TO
cord-297160-tqw9vx2b	12	4	determine	determine	VB
cord-297160-tqw9vx2b	12	5	end	end	NN
cord-297160-tqw9vx2b	12	6	-	-	HYPH
cord-297160-tqw9vx2b	12	7	points	point	NNS
cord-297160-tqw9vx2b	12	8	in	in	IN
cord-297160-tqw9vx2b	12	9	the	the	DT
cord-297160-tqw9vx2b	12	10	titration	titration	NN
cord-297160-tqw9vx2b	12	11	for	for	IN
cord-297160-tqw9vx2b	12	12	each	each	DT
cord-297160-tqw9vx2b	12	13	of	of	IN
cord-297160-tqw9vx2b	12	14	the	the	DT
cord-297160-tqw9vx2b	12	15	two	two	CD
cord-297160-tqw9vx2b	12	16	strains	strain	NNS
cord-297160-tqw9vx2b	12	17	,	,	,
cord-297160-tqw9vx2b	12	18	we	-PRON-	PRP
cord-297160-tqw9vx2b	12	19	collected	collect	VBD
cord-297160-tqw9vx2b	12	20	the	the	DT
cord-297160-tqw9vx2b	12	21	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	12	22	fluids	fluid	NNS
cord-297160-tqw9vx2b	12	23	from	from	IN
cord-297160-tqw9vx2b	12	24	each	each	DT
cord-297160-tqw9vx2b	12	25	egg	egg	NN
cord-297160-tqw9vx2b	12	26	after	after	IN
cord-297160-tqw9vx2b	12	27	the	the	DT
cord-297160-tqw9vx2b	12	28	incubation	incubation	NN
cord-297160-tqw9vx2b	12	29	period	period	NN
cord-297160-tqw9vx2b	12	30	and	and	CC
cord-297160-tqw9vx2b	12	31	tested	test	VBD
cord-297160-tqw9vx2b	12	32	these	these	DT
cord-297160-tqw9vx2b	12	33	for	for	IN
cord-297160-tqw9vx2b	12	34	the	the	DT
cord-297160-tqw9vx2b	12	35	presence	presence	NN
cord-297160-tqw9vx2b	12	36	of	of	IN
cord-297160-tqw9vx2b	12	37	IB	IB	NNP
cord-297160-tqw9vx2b	12	38	H120	H120	NNP
cord-297160-tqw9vx2b	12	39	and	and	CC
cord-297160-tqw9vx2b	12	40	IB	IB	NNP
cord-297160-tqw9vx2b	12	41	D274	D274	NNP
cord-297160-tqw9vx2b	12	42	by	by	IN
cord-297160-tqw9vx2b	12	43	a	a	DT
cord-297160-tqw9vx2b	12	44	strain	strain	NN
cord-297160-tqw9vx2b	12	45	specific	specific	JJ
cord-297160-tqw9vx2b	12	46	reverse	reverse	NN
cord-297160-tqw9vx2b	12	47	phase	phase	NN
cord-297160-tqw9vx2b	12	48	PCR	PCR	NNP
cord-297160-tqw9vx2b	12	49	.	.	.
cord-297160-tqw9vx2b	13	1	Based	base	VBN
cord-297160-tqw9vx2b	13	2	on	on	IN
cord-297160-tqw9vx2b	13	3	the	the	DT
cord-297160-tqw9vx2b	13	4	data	datum	NNS
cord-297160-tqw9vx2b	13	5	obtained	obtain	VBN
cord-297160-tqw9vx2b	13	6	by	by	IN
cord-297160-tqw9vx2b	13	7	PCR	PCR	NNP
cord-297160-tqw9vx2b	13	8	we	-PRON-	PRP
cord-297160-tqw9vx2b	13	9	were	be	VBD
cord-297160-tqw9vx2b	13	10	able	able	JJ
cord-297160-tqw9vx2b	13	11	to	to	TO
cord-297160-tqw9vx2b	13	12	determine	determine	VB
cord-297160-tqw9vx2b	13	13	an	an	DT
cord-297160-tqw9vx2b	13	14	end	end	NN
cord-297160-tqw9vx2b	13	15	-	-	HYPH
cord-297160-tqw9vx2b	13	16	point	point	NN
cord-297160-tqw9vx2b	13	17	for	for	IN
cord-297160-tqw9vx2b	13	18	each	each	DT
cord-297160-tqw9vx2b	13	19	of	of	IN
cord-297160-tqw9vx2b	13	20	the	the	DT
cord-297160-tqw9vx2b	13	21	two	two	CD
cord-297160-tqw9vx2b	13	22	strains	strain	NNS
cord-297160-tqw9vx2b	13	23	.	.	.
cord-297160-tqw9vx2b	14	1	For	for	IN
cord-297160-tqw9vx2b	14	2	a	a	DT
cord-297160-tqw9vx2b	14	3	given	give	VBN
cord-297160-tqw9vx2b	14	4	commercial	commercial	JJ
cord-297160-tqw9vx2b	14	5	batch	batch	NN
cord-297160-tqw9vx2b	14	6	of	of	IN
cord-297160-tqw9vx2b	14	7	Poulvac	Poulvac	NNP
cord-297160-tqw9vx2b	14	8	IB	IB	NNP
cord-297160-tqw9vx2b	14	9	primer	primer	NN
cord-297160-tqw9vx2b	14	10	we	-PRON-	PRP
cord-297160-tqw9vx2b	14	11	determined	determine	VBD
cord-297160-tqw9vx2b	14	12	titres	titre	NNS
cord-297160-tqw9vx2b	14	13	of	of	IN
cord-297160-tqw9vx2b	14	14	10(6.31	10(6.31	CD
cord-297160-tqw9vx2b	14	15	)	)	-RRB-
cord-297160-tqw9vx2b	14	16	EID(50	eid(50	NN
cord-297160-tqw9vx2b	14	17	)	)	-RRB-
cord-297160-tqw9vx2b	14	18	per	per	IN
cord-297160-tqw9vx2b	14	19	vial	vial	NN
cord-297160-tqw9vx2b	14	20	for	for	IN
cord-297160-tqw9vx2b	14	21	IB	IB	NNP
cord-297160-tqw9vx2b	14	22	H120	H120	NNP
cord-297160-tqw9vx2b	15	1	and	and	CC
cord-297160-tqw9vx2b	15	2	10(6.59	10(6.59	CD
cord-297160-tqw9vx2b	15	3	)	)	-RRB-
cord-297160-tqw9vx2b	15	4	EID(50	EID(50	NNP
cord-297160-tqw9vx2b	15	5	)	)	-RRB-
cord-297160-tqw9vx2b	15	6	for	for	IN
cord-297160-tqw9vx2b	15	7	IB	IB	NNP
cord-297160-tqw9vx2b	15	8	D274	D274	NNP
cord-297160-tqw9vx2b	15	9	using	use	VBG
cord-297160-tqw9vx2b	15	10	PCR	PCR	NNP
cord-297160-tqw9vx2b	15	11	for	for	IN
cord-297160-tqw9vx2b	15	12	end	end	NN
cord-297160-tqw9vx2b	15	13	-	-	HYPH
cord-297160-tqw9vx2b	15	14	point	point	NN
cord-297160-tqw9vx2b	15	15	determination	determination	NN
cord-297160-tqw9vx2b	15	16	.	.	.
cord-297160-tqw9vx2b	16	1	These	these	DT
cord-297160-tqw9vx2b	16	2	end	end	NN
cord-297160-tqw9vx2b	16	3	-	-	HYPH
cord-297160-tqw9vx2b	16	4	points	point	NNS
cord-297160-tqw9vx2b	16	5	matched	match	VBN
cord-297160-tqw9vx2b	16	6	well	well	RB
cord-297160-tqw9vx2b	16	7	with	with	IN
cord-297160-tqw9vx2b	16	8	the	the	DT
cord-297160-tqw9vx2b	16	9	end	end	NN
cord-297160-tqw9vx2b	16	10	-	-	HYPH
cord-297160-tqw9vx2b	16	11	point	point	NN
cord-297160-tqw9vx2b	16	12	determined	determine	VBN
cord-297160-tqw9vx2b	16	13	for	for	IN
cord-297160-tqw9vx2b	16	14	both	both	DT
cord-297160-tqw9vx2b	16	15	strains	strain	NNS
cord-297160-tqw9vx2b	16	16	cumulatively	cumulatively	RB
cord-297160-tqw9vx2b	16	17	after	after	IN
cord-297160-tqw9vx2b	16	18	visual	visual	JJ
cord-297160-tqw9vx2b	16	19	examination	examination	NN
cord-297160-tqw9vx2b	16	20	,	,	,
cord-297160-tqw9vx2b	16	21	i.e.	i.e.	FW
cord-297160-tqw9vx2b	16	22	10(6.67	10(6.67	CD
cord-297160-tqw9vx2b	16	23	)	)	-RRB-
cord-297160-tqw9vx2b	16	24	EID(50	eid(50	NN
cord-297160-tqw9vx2b	16	25	)	)	-RRB-
cord-297160-tqw9vx2b	16	26	per	per	IN
cord-297160-tqw9vx2b	16	27	vial	vial	NN
cord-297160-tqw9vx2b	16	28	.	.	.
cord-297160-tqw9vx2b	17	1	It	-PRON-	PRP
cord-297160-tqw9vx2b	17	2	is	be	VBZ
cord-297160-tqw9vx2b	17	3	concluded	conclude	VBN
cord-297160-tqw9vx2b	17	4	that	that	IN
cord-297160-tqw9vx2b	17	5	PCR	PCR	NNP
cord-297160-tqw9vx2b	17	6	is	be	VBZ
cord-297160-tqw9vx2b	17	7	a	a	DT
cord-297160-tqw9vx2b	17	8	suitable	suitable	JJ
cord-297160-tqw9vx2b	17	9	means	mean	NNS
cord-297160-tqw9vx2b	17	10	to	to	TO
cord-297160-tqw9vx2b	17	11	determine	determine	VB
cord-297160-tqw9vx2b	17	12	end	end	NN
cord-297160-tqw9vx2b	17	13	-	-	HYPH
cord-297160-tqw9vx2b	17	14	points	point	NNS
cord-297160-tqw9vx2b	17	15	in	in	IN
cord-297160-tqw9vx2b	17	16	titrations	titration	NNS
cord-297160-tqw9vx2b	17	17	of	of	IN
cord-297160-tqw9vx2b	17	18	live	live	JJ
cord-297160-tqw9vx2b	17	19	viruses	virus	NNS
cord-297160-tqw9vx2b	17	20	.	.	.
cord-297160-tqw9vx2b	18	1	Infectious	infectious	JJ
cord-297160-tqw9vx2b	18	2	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	18	3	virus	virus	NN
cord-297160-tqw9vx2b	18	4	(	(	-LRB-
cord-297160-tqw9vx2b	18	5	IBV	IBV	NNP
cord-297160-tqw9vx2b	18	6	)	)	-RRB-
cord-297160-tqw9vx2b	18	7	,	,	,
cord-297160-tqw9vx2b	18	8	a	a	DT
cord-297160-tqw9vx2b	18	9	coronavirus	coronavirus	NN
cord-297160-tqw9vx2b	18	10	,	,	,
cord-297160-tqw9vx2b	18	11	is	be	VBZ
cord-297160-tqw9vx2b	18	12	one	one	CD
cord-297160-tqw9vx2b	18	13	of	of	IN
cord-297160-tqw9vx2b	18	14	the	the	DT
cord-297160-tqw9vx2b	18	15	foremost	foremost	JJ
cord-297160-tqw9vx2b	18	16	causes	cause	NNS
cord-297160-tqw9vx2b	18	17	of	of	IN
cord-297160-tqw9vx2b	18	18	economic	economic	JJ
cord-297160-tqw9vx2b	18	19	losses	loss	NNS
cord-297160-tqw9vx2b	18	20	within	within	IN
cord-297160-tqw9vx2b	18	21	the	the	DT
cord-297160-tqw9vx2b	18	22	poultry	poultry	NN
cord-297160-tqw9vx2b	18	23	industry	industry	NN
cord-297160-tqw9vx2b	18	24	,	,	,
cord-297160-tqw9vx2b	18	25	affecting	affect	VBG
cord-297160-tqw9vx2b	18	26	the	the	DT
cord-297160-tqw9vx2b	18	27	performance	performance	NN
cord-297160-tqw9vx2b	18	28	of	of	IN
cord-297160-tqw9vx2b	18	29	both	both	DT
cord-297160-tqw9vx2b	18	30	meat	meat	NN
cord-297160-tqw9vx2b	18	31	-	-	HYPH
cord-297160-tqw9vx2b	18	32	providing	providing	NN
cord-297160-tqw9vx2b	18	33	and	and	CC
cord-297160-tqw9vx2b	18	34	egg	egg	NN
cord-297160-tqw9vx2b	18	35	-	-	HYPH
cord-297160-tqw9vx2b	18	36	laying	lay	VBG
cord-297160-tqw9vx2b	18	37	chickens	chicken	NNS
cord-297160-tqw9vx2b	18	38	(	(	-LRB-
cord-297160-tqw9vx2b	18	39	Cavanagh	Cavanagh	NNP
cord-297160-tqw9vx2b	18	40	,	,	,
cord-297160-tqw9vx2b	18	41	2007	2007	CD
cord-297160-tqw9vx2b	18	42	;	;	.
cord-297160-tqw9vx2b	19	1	De	De	NNP
cord-297160-tqw9vx2b	19	2	Wit	Wit	NNP
cord-297160-tqw9vx2b	19	3	et	et	NNP
cord-297160-tqw9vx2b	19	4	al	al	NNP
cord-297160-tqw9vx2b	19	5	.	.	NNP
cord-297160-tqw9vx2b	19	6	,	,	,
cord-297160-tqw9vx2b	19	7	2011	2011	CD
cord-297160-tqw9vx2b	19	8	)	)	-RRB-
cord-297160-tqw9vx2b	19	9	.	.	.
cord-297160-tqw9vx2b	20	1	The	the	DT
cord-297160-tqw9vx2b	20	2	major	major	JJ
cord-297160-tqw9vx2b	20	3	reason	reason	NN
cord-297160-tqw9vx2b	20	4	for	for	IN
cord-297160-tqw9vx2b	20	5	the	the	DT
cord-297160-tqw9vx2b	20	6	high	high	JJ
cord-297160-tqw9vx2b	20	7	profile	profile	NN
cord-297160-tqw9vx2b	20	8	of	of	IN
cord-297160-tqw9vx2b	20	9	IBV	IBV	NNP
cord-297160-tqw9vx2b	20	10	is	be	VBZ
cord-297160-tqw9vx2b	20	11	due	due	JJ
cord-297160-tqw9vx2b	20	12	to	to	IN
cord-297160-tqw9vx2b	20	13	the	the	DT
cord-297160-tqw9vx2b	20	14	existence	existence	NN
cord-297160-tqw9vx2b	20	15	of	of	IN
cord-297160-tqw9vx2b	20	16	a	a	DT
cord-297160-tqw9vx2b	20	17	very	very	RB
cord-297160-tqw9vx2b	20	18	large	large	JJ
cord-297160-tqw9vx2b	20	19	number	number	NN
cord-297160-tqw9vx2b	20	20	of	of	IN
cord-297160-tqw9vx2b	20	21	serotypes	serotype	NNS
cord-297160-tqw9vx2b	20	22	,	,	,
cord-297160-tqw9vx2b	20	23	although	although	IN
cord-297160-tqw9vx2b	20	24	the	the	DT
cord-297160-tqw9vx2b	20	25	virus	virus	NN
cord-297160-tqw9vx2b	20	26	is	be	VBZ
cord-297160-tqw9vx2b	20	27	also	also	RB
cord-297160-tqw9vx2b	20	28	highly	highly	RB
cord-297160-tqw9vx2b	20	29	contagious	contagious	JJ
cord-297160-tqw9vx2b	20	30	.	.	.
cord-297160-tqw9vx2b	21	1	In	in	IN
cord-297160-tqw9vx2b	21	2	order	order	NN
cord-297160-tqw9vx2b	21	3	to	to	TO
cord-297160-tqw9vx2b	21	4	control	control	VB
cord-297160-tqw9vx2b	21	5	the	the	DT
cord-297160-tqw9vx2b	21	6	disease	disease	NN
cord-297160-tqw9vx2b	21	7	in	in	IN
cord-297160-tqw9vx2b	21	8	poultry	poultry	NN
cord-297160-tqw9vx2b	21	9	,	,	,
cord-297160-tqw9vx2b	21	10	both	both	DT
cord-297160-tqw9vx2b	21	11	live	live	VBP
cord-297160-tqw9vx2b	21	12	and	and	CC
cord-297160-tqw9vx2b	21	13	inactivated	inactivate	VBD
cord-297160-tqw9vx2b	21	14	IB	IB	NNP
cord-297160-tqw9vx2b	21	15	vaccines	vaccine	NNS
cord-297160-tqw9vx2b	21	16	are	be	VBP
cord-297160-tqw9vx2b	21	17	used	use	VBN
cord-297160-tqw9vx2b	21	18	extensively	extensively	RB
cord-297160-tqw9vx2b	21	19	.	.	.
cord-297160-tqw9vx2b	22	1	The	the	DT
cord-297160-tqw9vx2b	22	2	nature	nature	NN
cord-297160-tqw9vx2b	22	3	of	of	IN
cord-297160-tqw9vx2b	22	4	the	the	DT
cord-297160-tqw9vx2b	22	5	protective	protective	JJ
cord-297160-tqw9vx2b	22	6	immune	immune	JJ
cord-297160-tqw9vx2b	22	7	response	response	NN
cord-297160-tqw9vx2b	22	8	to	to	IN
cord-297160-tqw9vx2b	22	9	IBV	IBV	NNP
cord-297160-tqw9vx2b	22	10	is	be	VBZ
cord-297160-tqw9vx2b	22	11	poorly	poorly	RB
cord-297160-tqw9vx2b	22	12	understood	understand	VBN
cord-297160-tqw9vx2b	22	13	,	,	,
cord-297160-tqw9vx2b	22	14	but	but	CC
cord-297160-tqw9vx2b	22	15	research	research	NN
cord-297160-tqw9vx2b	22	16	has	have	VBZ
cord-297160-tqw9vx2b	22	17	demonstrated	demonstrate	VBN
cord-297160-tqw9vx2b	22	18	that	that	IN
cord-297160-tqw9vx2b	22	19	the	the	DT
cord-297160-tqw9vx2b	22	20	surface	surface	NN
cord-297160-tqw9vx2b	22	21	spike	spike	NN
cord-297160-tqw9vx2b	22	22	protein	protein	NN
cord-297160-tqw9vx2b	22	23	,	,	,
cord-297160-tqw9vx2b	22	24	and	and	CC
cord-297160-tqw9vx2b	22	25	particularly	particularly	RB
cord-297160-tqw9vx2b	22	26	the	the	DT
cord-297160-tqw9vx2b	22	27	amino	amino	NN
cord-297160-tqw9vx2b	22	28	-	-	HYPH
cord-297160-tqw9vx2b	22	29	terminal	terminal	NN
cord-297160-tqw9vx2b	22	30	S1	s1	NN
cord-297160-tqw9vx2b	22	31	portion	portion	NN
cord-297160-tqw9vx2b	22	32	,	,	,
cord-297160-tqw9vx2b	22	33	is	be	VBZ
cord-297160-tqw9vx2b	22	34	sufficient	sufficient	JJ
cord-297160-tqw9vx2b	22	35	to	to	TO
cord-297160-tqw9vx2b	22	36	induce	induce	VB
cord-297160-tqw9vx2b	22	37	good	good	JJ
cord-297160-tqw9vx2b	22	38	protective	protective	JJ
cord-297160-tqw9vx2b	22	39	immunity	immunity	NN
cord-297160-tqw9vx2b	22	40	.	.	.
cord-297160-tqw9vx2b	23	1	There	there	EX
cord-297160-tqw9vx2b	23	2	is	be	VBZ
cord-297160-tqw9vx2b	23	3	increasing	increase	VBG
cord-297160-tqw9vx2b	23	4	evidence	evidence	NN
cord-297160-tqw9vx2b	23	5	that	that	IN
cord-297160-tqw9vx2b	23	6	only	only	RB
cord-297160-tqw9vx2b	23	7	a	a	DT
cord-297160-tqw9vx2b	23	8	few	few	JJ
cord-297160-tqw9vx2b	23	9	amino	amino	NN
cord-297160-tqw9vx2b	23	10	acid	acid	NN
cord-297160-tqw9vx2b	23	11	differences	difference	NNS
cord-297160-tqw9vx2b	23	12	amongst	amongst	IN
cord-297160-tqw9vx2b	23	13	S	s	NN
cord-297160-tqw9vx2b	23	14	proteins	protein	NNS
cord-297160-tqw9vx2b	23	15	are	be	VBP
cord-297160-tqw9vx2b	23	16	sufficient	sufficient	JJ
cord-297160-tqw9vx2b	23	17	to	to	TO
cord-297160-tqw9vx2b	23	18	have	have	VB
cord-297160-tqw9vx2b	23	19	a	a	DT
cord-297160-tqw9vx2b	23	20	detrimental	detrimental	JJ
cord-297160-tqw9vx2b	23	21	impact	impact	NN
cord-297160-tqw9vx2b	23	22	on	on	IN
cord-297160-tqw9vx2b	23	23	cross	cross	NN
cord-297160-tqw9vx2b	23	24	-	-	NN
cord-297160-tqw9vx2b	23	25	protection	protection	JJ
cord-297160-tqw9vx2b	23	26	(	(	-LRB-
cord-297160-tqw9vx2b	23	27	Cavanagh	Cavanagh	NNP
cord-297160-tqw9vx2b	23	28	,	,	,
cord-297160-tqw9vx2b	23	29	2007	2007	CD
cord-297160-tqw9vx2b	23	30	)	)	-RRB-
cord-297160-tqw9vx2b	23	31	.	.	.
cord-297160-tqw9vx2b	24	1	As	as	IN
cord-297160-tqw9vx2b	24	2	it	-PRON-	PRP
cord-297160-tqw9vx2b	24	3	is	be	VBZ
cord-297160-tqw9vx2b	24	4	not	not	RB
cord-297160-tqw9vx2b	24	5	always	always	RB
cord-297160-tqw9vx2b	24	6	clear	clear	JJ
cord-297160-tqw9vx2b	24	7	which	which	WDT
cord-297160-tqw9vx2b	24	8	serotype	serotype	NN
cord-297160-tqw9vx2b	24	9	is	be	VBZ
cord-297160-tqw9vx2b	24	10	responsible	responsible	JJ
cord-297160-tqw9vx2b	24	11	for	for	IN
cord-297160-tqw9vx2b	24	12	any	any	DT
cord-297160-tqw9vx2b	24	13	health	health	NN
cord-297160-tqw9vx2b	24	14	problems	problem	NNS
cord-297160-tqw9vx2b	24	15	in	in	IN
cord-297160-tqw9vx2b	24	16	poultry	poultry	NN
cord-297160-tqw9vx2b	24	17	,	,	,
cord-297160-tqw9vx2b	24	18	combinations	combination	NNS
cord-297160-tqw9vx2b	24	19	of	of	IN
cord-297160-tqw9vx2b	24	20	vaccines	vaccine	NNS
cord-297160-tqw9vx2b	24	21	are	be	VBP
cord-297160-tqw9vx2b	24	22	widely	widely	RB
cord-297160-tqw9vx2b	24	23	used	use	VBN
cord-297160-tqw9vx2b	24	24	to	to	TO
cord-297160-tqw9vx2b	24	25	generate	generate	VB
cord-297160-tqw9vx2b	24	26	broad	broad	JJ
cord-297160-tqw9vx2b	24	27	protection	protection	NN
cord-297160-tqw9vx2b	25	1	*	*	NFP
cord-297160-tqw9vx2b	25	2	Corresponding	correspond	VBG
cord-297160-tqw9vx2b	25	3	author	author	NN
cord-297160-tqw9vx2b	25	4	.	.	.
cord-297160-tqw9vx2b	26	1	Tel	Tel	NNP
cord-297160-tqw9vx2b	26	2	.	.	.
cord-297160-tqw9vx2b	27	1	:	:	:
cord-297160-tqw9vx2b	27	2	+32	+32	NNP
cord-297160-tqw9vx2b	27	3	2	2	CD
cord-297160-tqw9vx2b	27	4	7157593	7157593	CD
cord-297160-tqw9vx2b	27	5	;	;	:
cord-297160-tqw9vx2b	28	1	fax	fax	NN
cord-297160-tqw9vx2b	28	2	:	:	:
cord-297160-tqw9vx2b	28	3	+32	+32	NN
cord-297160-tqw9vx2b	28	4	2	2	CD
cord-297160-tqw9vx2b	28	5	4012407	4012407	CD
cord-297160-tqw9vx2b	28	6	.	.	.
cord-297160-tqw9vx2b	29	1	E	e	NN
cord-297160-tqw9vx2b	29	2	-	-	NN
cord-297160-tqw9vx2b	29	3	mail	mail	NN
cord-297160-tqw9vx2b	29	4	addresses	address	NNS
cord-297160-tqw9vx2b	29	5	:	:	:
cord-297160-tqw9vx2b	29	6	harm.geerligs@zoetis.com	harm.geerligs@zoetis.com	ADD
cord-297160-tqw9vx2b	29	7	,	,	,
cord-297160-tqw9vx2b	29	8	harm.geerligs@pfizer.com	harm.geerligs@pfizer.com	ADD
cord-297160-tqw9vx2b	29	9	(	(	-LRB-
cord-297160-tqw9vx2b	29	10	H.J.	H.J.	NNP
cord-297160-tqw9vx2b	29	11	Geerligs	Geerligs	NNP
cord-297160-tqw9vx2b	29	12	)	)	-RRB-
cord-297160-tqw9vx2b	29	13	.	.	.
cord-297160-tqw9vx2b	30	1	(	(	-LRB-
cord-297160-tqw9vx2b	30	2	Terregino	Terregino	NNP
cord-297160-tqw9vx2b	30	3	et	et	NNP
cord-297160-tqw9vx2b	30	4	al	al	NNP
cord-297160-tqw9vx2b	30	5	.	.	NNP
cord-297160-tqw9vx2b	30	6	,	,	,
cord-297160-tqw9vx2b	30	7	2008	2008	CD
cord-297160-tqw9vx2b	30	8	)	)	-RRB-
cord-297160-tqw9vx2b	30	9	.	.	.
cord-297160-tqw9vx2b	31	1	Another	another	DT
cord-297160-tqw9vx2b	31	2	possibility	possibility	NN
cord-297160-tqw9vx2b	31	3	is	be	VBZ
cord-297160-tqw9vx2b	31	4	to	to	TO
cord-297160-tqw9vx2b	31	5	use	use	VB
cord-297160-tqw9vx2b	31	6	vaccines	vaccine	NNS
cord-297160-tqw9vx2b	31	7	which	which	WDT
cord-297160-tqw9vx2b	31	8	contain	contain	VBP
cord-297160-tqw9vx2b	31	9	a	a	DT
cord-297160-tqw9vx2b	31	10	combination	combination	NN
cord-297160-tqw9vx2b	31	11	of	of	IN
cord-297160-tqw9vx2b	31	12	different	different	JJ
cord-297160-tqw9vx2b	31	13	vaccine	vaccine	NN
cord-297160-tqw9vx2b	31	14	viral	viral	JJ
cord-297160-tqw9vx2b	31	15	strains	strain	NNS
cord-297160-tqw9vx2b	31	16	.	.	.
cord-297160-tqw9vx2b	32	1	Zoetis	Zoetis	NNP
cord-297160-tqw9vx2b	32	2	is	be	VBZ
cord-297160-tqw9vx2b	32	3	marketing	market	VBG
cord-297160-tqw9vx2b	32	4	a	a	DT
cord-297160-tqw9vx2b	32	5	live	live	JJ
cord-297160-tqw9vx2b	32	6	combination	combination	NN
cord-297160-tqw9vx2b	32	7	vaccine	vaccine	NN
cord-297160-tqw9vx2b	32	8	for	for	IN
cord-297160-tqw9vx2b	32	9	chickens	chicken	NNS
cord-297160-tqw9vx2b	32	10	named	name	VBN
cord-297160-tqw9vx2b	32	11	Poulvac	Poulvac	NNP
cord-297160-tqw9vx2b	32	12	®	®	.
cord-297160-tqw9vx2b	32	13	IB	IB	NNP
cord-297160-tqw9vx2b	32	14	Primer	Primer	NNP
cord-297160-tqw9vx2b	32	15	.	.	.
cord-297160-tqw9vx2b	33	1	This	this	DT
cord-297160-tqw9vx2b	33	2	lyophilized	lyophilized	JJ
cord-297160-tqw9vx2b	33	3	vaccine	vaccine	NN
cord-297160-tqw9vx2b	33	4	contains	contain	VBZ
cord-297160-tqw9vx2b	33	5	two	two	CD
cord-297160-tqw9vx2b	33	6	attenuated	attenuated	JJ
cord-297160-tqw9vx2b	33	7	infectious	infectious	JJ
cord-297160-tqw9vx2b	33	8	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	33	9	strains	strain	NNS
cord-297160-tqw9vx2b	33	10	,	,	,
cord-297160-tqw9vx2b	33	11	IB	IB	NNP
cord-297160-tqw9vx2b	33	12	H120	H120	NNP
cord-297160-tqw9vx2b	34	1	(	(	-LRB-
cord-297160-tqw9vx2b	34	2	MacDonald	MacDonald	NNP
cord-297160-tqw9vx2b	34	3	and	and	CC
cord-297160-tqw9vx2b	34	4	McMartin	McMartin	NNP
cord-297160-tqw9vx2b	34	5	,	,	,
cord-297160-tqw9vx2b	34	6	1976	1976	CD
cord-297160-tqw9vx2b	34	7	)	)	-RRB-
cord-297160-tqw9vx2b	34	8	and	and	CC
cord-297160-tqw9vx2b	34	9	IB	IB	NNP
cord-297160-tqw9vx2b	34	10	D274	D274	NNP
cord-297160-tqw9vx2b	35	1	(	(	-LRB-
cord-297160-tqw9vx2b	35	2	Kusters	Kusters	NNPS
cord-297160-tqw9vx2b	35	3	et	et	NNP
cord-297160-tqw9vx2b	35	4	al	al	NNP
cord-297160-tqw9vx2b	35	5	.	.	NNP
cord-297160-tqw9vx2b	35	6	,	,	,
cord-297160-tqw9vx2b	35	7	1987	1987	CD
cord-297160-tqw9vx2b	35	8	)	)	-RRB-
cord-297160-tqw9vx2b	35	9	.	.	.
cord-297160-tqw9vx2b	36	1	Both	both	DT
cord-297160-tqw9vx2b	36	2	vaccine	vaccine	NN
cord-297160-tqw9vx2b	36	3	strains	strain	NNS
cord-297160-tqw9vx2b	36	4	have	have	VBP
cord-297160-tqw9vx2b	36	5	been	be	VBN
cord-297160-tqw9vx2b	36	6	attenuated	attenuate	VBN
cord-297160-tqw9vx2b	36	7	by	by	IN
cord-297160-tqw9vx2b	36	8	passage	passage	NN
cord-297160-tqw9vx2b	36	9	in	in	IN
cord-297160-tqw9vx2b	36	10	embryonated	embryonate	VBN
cord-297160-tqw9vx2b	36	11	chicken	chicken	NN
cord-297160-tqw9vx2b	36	12	eggs	egg	NNS
cord-297160-tqw9vx2b	36	13	,	,	,
cord-297160-tqw9vx2b	36	14	which	which	WDT
cord-297160-tqw9vx2b	36	15	is	be	VBZ
cord-297160-tqw9vx2b	36	16	the	the	DT
cord-297160-tqw9vx2b	36	17	usual	usual	JJ
cord-297160-tqw9vx2b	36	18	method	method	NN
cord-297160-tqw9vx2b	36	19	for	for	IN
cord-297160-tqw9vx2b	36	20	attenuation	attenuation	NN
cord-297160-tqw9vx2b	36	21	of	of	IN
cord-297160-tqw9vx2b	36	22	infectious	infectious	JJ
cord-297160-tqw9vx2b	36	23	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	36	24	viruses	virus	NNS
cord-297160-tqw9vx2b	36	25	and	and	CC
cord-297160-tqw9vx2b	36	26	development	development	NN
cord-297160-tqw9vx2b	36	27	of	of	IN
cord-297160-tqw9vx2b	36	28	vaccines	vaccine	NNS
cord-297160-tqw9vx2b	36	29	(	(	-LRB-
cord-297160-tqw9vx2b	36	30	MacDonald	MacDonald	NNP
cord-297160-tqw9vx2b	36	31	and	and	CC
cord-297160-tqw9vx2b	36	32	McMartin	McMartin	NNP
cord-297160-tqw9vx2b	36	33	,	,	,
cord-297160-tqw9vx2b	36	34	1976	1976	CD
cord-297160-tqw9vx2b	36	35	;	;	:
cord-297160-tqw9vx2b	36	36	Klieve	Klieve	NNP
cord-297160-tqw9vx2b	36	37	and	and	CC
cord-297160-tqw9vx2b	36	38	Cummings	Cummings	NNP
cord-297160-tqw9vx2b	36	39	,	,	,
cord-297160-tqw9vx2b	36	40	1988	1988	CD
cord-297160-tqw9vx2b	36	41	;	;	:
cord-297160-tqw9vx2b	37	1	Jackwood	Jackwood	NNP
cord-297160-tqw9vx2b	37	2	et	et	NNP
cord-297160-tqw9vx2b	37	3	al	al	NNP
cord-297160-tqw9vx2b	37	4	.	.	.
cord-297160-tqw9vx2b	38	1	,	,	,
cord-297160-tqw9vx2b	38	2	2003	2003	CD
cord-297160-tqw9vx2b	38	3	;	;	:
cord-297160-tqw9vx2b	38	4	Liu	Liu	NNP
cord-297160-tqw9vx2b	38	5	et	et	NNP
cord-297160-tqw9vx2b	38	6	al	al	NNP
cord-297160-tqw9vx2b	38	7	.	.	NNP
cord-297160-tqw9vx2b	38	8	,	,	,
cord-297160-tqw9vx2b	38	9	2007	2007	CD
cord-297160-tqw9vx2b	38	10	)	)	-RRB-
cord-297160-tqw9vx2b	38	11	.	.	.
cord-297160-tqw9vx2b	39	1	In	in	IN
cord-297160-tqw9vx2b	39	2	order	order	NN
cord-297160-tqw9vx2b	39	3	to	to	TO
cord-297160-tqw9vx2b	39	4	quantify	quantify	VB
cord-297160-tqw9vx2b	39	5	the	the	DT
cord-297160-tqw9vx2b	39	6	viral	viral	JJ
cord-297160-tqw9vx2b	39	7	content	content	NN
cord-297160-tqw9vx2b	39	8	of	of	IN
cord-297160-tqw9vx2b	39	9	the	the	DT
cord-297160-tqw9vx2b	39	10	vaccine	vaccine	NN
cord-297160-tqw9vx2b	39	11	,	,	,
cord-297160-tqw9vx2b	39	12	dilution	dilution	NN
cord-297160-tqw9vx2b	39	13	series	series	NN
cord-297160-tqw9vx2b	39	14	of	of	IN
cord-297160-tqw9vx2b	39	15	the	the	DT
cord-297160-tqw9vx2b	39	16	final	final	JJ
cord-297160-tqw9vx2b	39	17	product	product	NN
cord-297160-tqw9vx2b	39	18	are	be	VBP
cord-297160-tqw9vx2b	39	19	inoculated	inoculate	VBN
cord-297160-tqw9vx2b	39	20	in	in	IN
cord-297160-tqw9vx2b	39	21	10	10	CD
cord-297160-tqw9vx2b	39	22	days	day	NNS
cord-297160-tqw9vx2b	39	23	embryonated	embryonate	VBN
cord-297160-tqw9vx2b	39	24	chicken	chicken	NN
cord-297160-tqw9vx2b	39	25	eggs	egg	NNS
cord-297160-tqw9vx2b	39	26	.	.	.
cord-297160-tqw9vx2b	40	1	After	after	IN
cord-297160-tqw9vx2b	40	2	the	the	DT
cord-297160-tqw9vx2b	40	3	incubation	incubation	NN
cord-297160-tqw9vx2b	40	4	period	period	NN
cord-297160-tqw9vx2b	40	5	the	the	DT
cord-297160-tqw9vx2b	40	6	eggs	egg	NNS
cord-297160-tqw9vx2b	40	7	are	be	VBP
cord-297160-tqw9vx2b	40	8	opened	open	VBN
cord-297160-tqw9vx2b	40	9	and	and	CC
cord-297160-tqw9vx2b	40	10	the	the	DT
cord-297160-tqw9vx2b	40	11	embryos	embryo	NNS
cord-297160-tqw9vx2b	40	12	removed	remove	VBN
cord-297160-tqw9vx2b	40	13	for	for	IN
cord-297160-tqw9vx2b	40	14	examination	examination	NN
cord-297160-tqw9vx2b	40	15	of	of	IN
cord-297160-tqw9vx2b	40	16	IB	IB	NNP
cord-297160-tqw9vx2b	40	17	specific	specific	JJ
cord-297160-tqw9vx2b	40	18	lesions	lesion	NNS
cord-297160-tqw9vx2b	40	19	.	.	.
cord-297160-tqw9vx2b	41	1	The	the	DT
cord-297160-tqw9vx2b	41	2	product	product	NN
cord-297160-tqw9vx2b	41	3	titre	titre	NN
cord-297160-tqw9vx2b	41	4	defined	define	VBN
cord-297160-tqw9vx2b	41	5	is	be	VBZ
cord-297160-tqw9vx2b	41	6	the	the	DT
cord-297160-tqw9vx2b	41	7	reciprocal	reciprocal	JJ
cord-297160-tqw9vx2b	41	8	value	value	NN
cord-297160-tqw9vx2b	41	9	of	of	IN
cord-297160-tqw9vx2b	41	10	the	the	DT
cord-297160-tqw9vx2b	41	11	dilution	dilution	NN
cord-297160-tqw9vx2b	41	12	causing	cause	VBG
cord-297160-tqw9vx2b	41	13	embryonal	embryonal	JJ
cord-297160-tqw9vx2b	41	14	lesions	lesion	NNS
cord-297160-tqw9vx2b	41	15	in	in	IN
cord-297160-tqw9vx2b	41	16	50	50	CD
cord-297160-tqw9vx2b	41	17	%	%	NN
cord-297160-tqw9vx2b	41	18	of	of	IN
cord-297160-tqw9vx2b	41	19	the	the	DT
cord-297160-tqw9vx2b	41	20	eggs	egg	NNS
cord-297160-tqw9vx2b	41	21	.	.	.
cord-297160-tqw9vx2b	42	1	For	for	IN
cord-297160-tqw9vx2b	42	2	the	the	DT
cord-297160-tqw9vx2b	42	3	combination	combination	NN
cord-297160-tqw9vx2b	42	4	vaccine	vaccine	NN
cord-297160-tqw9vx2b	43	1	Poulvac	Poulvac	NNP
cord-297160-tqw9vx2b	43	2	®	®	,
cord-297160-tqw9vx2b	43	3	IB	IB	NNP
cord-297160-tqw9vx2b	43	4	Primer	Primer	NNP
cord-297160-tqw9vx2b	43	5	the	the	DT
cord-297160-tqw9vx2b	43	6	result	result	NN
cord-297160-tqw9vx2b	43	7	is	be	VBZ
cord-297160-tqw9vx2b	43	8	a	a	DT
cord-297160-tqw9vx2b	43	9	cumulative	cumulative	JJ
cord-297160-tqw9vx2b	43	10	titre	titre	NN
cord-297160-tqw9vx2b	43	11	to	to	TO
cord-297160-tqw9vx2b	43	12	which	which	WDT
cord-297160-tqw9vx2b	43	13	both	both	DT
cord-297160-tqw9vx2b	43	14	IB	IB	NNP
cord-297160-tqw9vx2b	43	15	strains	strain	NNS
cord-297160-tqw9vx2b	43	16	contribute	contribute	VBP
cord-297160-tqw9vx2b	43	17	.	.	.
cord-297160-tqw9vx2b	44	1	In	in	IN
cord-297160-tqw9vx2b	44	2	order	order	NN
cord-297160-tqw9vx2b	44	3	to	to	TO
cord-297160-tqw9vx2b	44	4	titrate	titrate	VB
cord-297160-tqw9vx2b	44	5	both	both	DT
cord-297160-tqw9vx2b	44	6	virus	virus	NN
cord-297160-tqw9vx2b	44	7	strains	strain	NNS
cord-297160-tqw9vx2b	44	8	separately	separately	RB
cord-297160-tqw9vx2b	44	9	,	,	,
cord-297160-tqw9vx2b	44	10	strain	strain	VBP
cord-297160-tqw9vx2b	44	11	specific	specific	JJ
cord-297160-tqw9vx2b	44	12	neutralizing	neutralize	VBG
cord-297160-tqw9vx2b	44	13	antisera	antisera	NN
cord-297160-tqw9vx2b	44	14	have	have	VBP
cord-297160-tqw9vx2b	44	15	been	be	VBN
cord-297160-tqw9vx2b	44	16	tested	test	VBN
cord-297160-tqw9vx2b	44	17	.	.	.
cord-297160-tqw9vx2b	45	1	It	-PRON-	PRP
cord-297160-tqw9vx2b	45	2	has	have	VBZ
cord-297160-tqw9vx2b	45	3	been	be	VBN
cord-297160-tqw9vx2b	45	4	shown	show	VBN
cord-297160-tqw9vx2b	45	5	that	that	DT
cord-297160-tqw9vx2b	45	6	antiserum	antiserum	NN
cord-297160-tqw9vx2b	45	7	against	against	IN
cord-297160-tqw9vx2b	45	8	strain	strain	NN
cord-297160-tqw9vx2b	45	9	IB	IB	NNP
cord-297160-tqw9vx2b	45	10	D274	D274	NNP
cord-297160-tqw9vx2b	45	11	does	do	VBZ
cord-297160-tqw9vx2b	45	12	not	not	RB
cord-297160-tqw9vx2b	45	13	cross	cross	VB
cord-297160-tqw9vx2b	45	14	-	-	JJ
cord-297160-tqw9vx2b	45	15	react	react	VB
cord-297160-tqw9vx2b	45	16	with	with	IN
cord-297160-tqw9vx2b	45	17	strain	strain	NN
cord-297160-tqw9vx2b	45	18	IB	IB	NNP
cord-297160-tqw9vx2b	45	19	H120	H120	NNP
cord-297160-tqw9vx2b	46	1	(	(	-LRB-
cord-297160-tqw9vx2b	46	2	Kusters	Kusters	NNPS
cord-297160-tqw9vx2b	46	3	et	et	NNP
cord-297160-tqw9vx2b	46	4	al	al	NNP
cord-297160-tqw9vx2b	46	5	.	.	NNP
cord-297160-tqw9vx2b	46	6	,	,	,
cord-297160-tqw9vx2b	46	7	1987	1987	CD
cord-297160-tqw9vx2b	46	8	)	)	-RRB-
cord-297160-tqw9vx2b	47	1	and	and	CC
cord-297160-tqw9vx2b	47	2	it	-PRON-	PRP
cord-297160-tqw9vx2b	47	3	was	be	VBD
cord-297160-tqw9vx2b	47	4	speculated	speculate	VBN
cord-297160-tqw9vx2b	47	5	that	that	IN
cord-297160-tqw9vx2b	47	6	antiserum	antiserum	NN
cord-297160-tqw9vx2b	47	7	against	against	IN
cord-297160-tqw9vx2b	47	8	IB	IB	NNP
cord-297160-tqw9vx2b	47	9	H120	H120	NNP
cord-297160-tqw9vx2b	47	10	would	would	MD
cord-297160-tqw9vx2b	47	11	not	not	RB
cord-297160-tqw9vx2b	47	12	cross	cross	VB
cord-297160-tqw9vx2b	47	13	react	react	VB
cord-297160-tqw9vx2b	47	14	with	with	IN
cord-297160-tqw9vx2b	47	15	strain	strain	NN
cord-297160-tqw9vx2b	47	16	IB	IB	NNP
cord-297160-tqw9vx2b	47	17	D274	D274	NNP
cord-297160-tqw9vx2b	47	18	.	.	.
cord-297160-tqw9vx2b	48	1	However	however	RB
cord-297160-tqw9vx2b	48	2	,	,	,
cord-297160-tqw9vx2b	48	3	during	during	IN
cord-297160-tqw9vx2b	48	4	titration	titration	NN
cord-297160-tqw9vx2b	48	5	experiments	experiment	NNS
cord-297160-tqw9vx2b	48	6	it	-PRON-	PRP
cord-297160-tqw9vx2b	48	7	was	be	VBD
cord-297160-tqw9vx2b	48	8	determined	determine	VBN
cord-297160-tqw9vx2b	48	9	Table	table	NN
cord-297160-tqw9vx2b	48	10	1	1	CD
cord-297160-tqw9vx2b	48	11	PCR	PCR	NNP
cord-297160-tqw9vx2b	48	12	primers	primer	NNS
cord-297160-tqw9vx2b	48	13	for	for	IN
cord-297160-tqw9vx2b	48	14	detection	detection	NN
cord-297160-tqw9vx2b	48	15	of	of	IN
cord-297160-tqw9vx2b	48	16	the	the	DT
cord-297160-tqw9vx2b	48	17	strains	strain	NNS
cord-297160-tqw9vx2b	48	18	IB	IB	NNP
cord-297160-tqw9vx2b	48	19	H120	H120	NNP
cord-297160-tqw9vx2b	48	20	and	and	CC
cord-297160-tqw9vx2b	48	21	IB	IB	NNP
cord-297160-tqw9vx2b	48	22	D274	D274	NNP
cord-297160-tqw9vx2b	48	23	,	,	,
cord-297160-tqw9vx2b	48	24	with	with	IN
cord-297160-tqw9vx2b	48	25	the	the	DT
cord-297160-tqw9vx2b	48	26	respective	respective	JJ
cord-297160-tqw9vx2b	48	27	PCR	PCR	NNP
cord-297160-tqw9vx2b	48	28	product	product	NN
cord-297160-tqw9vx2b	48	29	size	size	NN
cord-297160-tqw9vx2b	48	30	in	in	IN
cord-297160-tqw9vx2b	48	31	base	base	NN
cord-297160-tqw9vx2b	48	32	pairs	pair	NNS
cord-297160-tqw9vx2b	48	33	.	.	.
cord-297160-tqw9vx2b	49	1	Sequence	sequence	NN
cord-297160-tqw9vx2b	49	2	5	5	CD
cord-297160-tqw9vx2b	49	3	-3	-3	NNP
cord-297160-tqw9vx2b	49	4	forward	forward	NNP
cord-297160-tqw9vx2b	49	5	primer	primer	NNP
cord-297160-tqw9vx2b	49	6	Sequence	Sequence	NNP
cord-297160-tqw9vx2b	49	7	5	5	CD
cord-297160-tqw9vx2b	49	8	-3	-3	NNP
cord-297160-tqw9vx2b	49	9	reverse	reverse	NNP
cord-297160-tqw9vx2b	49	10	primer	primer	NN
cord-297160-tqw9vx2b	49	11	Length	length	NN
cord-297160-tqw9vx2b	49	12	in	in	IN
cord-297160-tqw9vx2b	49	13	base	base	NN
cord-297160-tqw9vx2b	49	14	pairs	pair	NNS
cord-297160-tqw9vx2b	49	15	IB	IB	NNP
cord-297160-tqw9vx2b	49	16	D274	D274	NNP
cord-297160-tqw9vx2b	49	17	5	5	CD
cord-297160-tqw9vx2b	49	18	-ATACAATTATATCAAACCAGC-3	-ATACAATTATATCAAACCAGC-3	NNP
cord-297160-tqw9vx2b	49	19	5	5	CD
cord-297160-tqw9vx2b	49	20	-AATACAGATTGCTTACAACCACC-3	-AATACAGATTGCTTACAACCACC-3	NNP
cord-297160-tqw9vx2b	49	21	211	211	CD
cord-297160-tqw9vx2b	49	22	IB	IB	NNP
cord-297160-tqw9vx2b	49	23	H120	H120	NNP
cord-297160-tqw9vx2b	50	1	5	5	CD
cord-297160-tqw9vx2b	50	2	-AATACTACTTTTACGTTACAC-3	-aatactacttttacgttacac-3	NN
cord-297160-tqw9vx2b	50	3	5	5	CD
cord-297160-tqw9vx2b	50	4	-AATACAGATTGCTTACAACCACC-3	-AATACAGATTGCTTACAACCACC-3	NNP
cord-297160-tqw9vx2b	50	5	299	299	CD
cord-297160-tqw9vx2b	51	1	that	that	IN
cord-297160-tqw9vx2b	51	2	the	the	DT
cord-297160-tqw9vx2b	51	3	antiserum	antiserum	NN
cord-297160-tqw9vx2b	51	4	against	against	IN
cord-297160-tqw9vx2b	51	5	IB	IB	NNP
cord-297160-tqw9vx2b	51	6	H120	H120	NNP
cord-297160-tqw9vx2b	51	7	strongly	strongly	RB
cord-297160-tqw9vx2b	51	8	reacted	react	VBD
cord-297160-tqw9vx2b	51	9	with	with	IN
cord-297160-tqw9vx2b	51	10	IB	IB	NNP
cord-297160-tqw9vx2b	51	11	D274	D274	NNP
cord-297160-tqw9vx2b	51	12	.	.	.
cord-297160-tqw9vx2b	52	1	PCR	PCR	NNP
cord-297160-tqw9vx2b	52	2	has	have	VBZ
cord-297160-tqw9vx2b	52	3	been	be	VBN
cord-297160-tqw9vx2b	52	4	used	use	VBN
cord-297160-tqw9vx2b	52	5	successfully	successfully	RB
cord-297160-tqw9vx2b	52	6	to	to	TO
cord-297160-tqw9vx2b	52	7	detect	detect	VB
cord-297160-tqw9vx2b	52	8	IB	IB	NNP
cord-297160-tqw9vx2b	52	9	strains	strain	NNS
cord-297160-tqw9vx2b	52	10	in	in	IN
cord-297160-tqw9vx2b	52	11	various	various	JJ
cord-297160-tqw9vx2b	52	12	samples	sample	NNS
cord-297160-tqw9vx2b	52	13	and	and	CC
cord-297160-tqw9vx2b	52	14	it	-PRON-	PRP
cord-297160-tqw9vx2b	52	15	appeared	appear	VBD
cord-297160-tqw9vx2b	52	16	to	to	TO
cord-297160-tqw9vx2b	52	17	be	be	VB
cord-297160-tqw9vx2b	52	18	possible	possible	JJ
cord-297160-tqw9vx2b	52	19	to	to	TO
cord-297160-tqw9vx2b	52	20	distinguish	distinguish	VB
cord-297160-tqw9vx2b	52	21	IB	IB	NNP
cord-297160-tqw9vx2b	52	22	strains	strain	NNS
cord-297160-tqw9vx2b	52	23	,	,	,
cord-297160-tqw9vx2b	52	24	including	include	VBG
cord-297160-tqw9vx2b	52	25	IB	IB	NNP
cord-297160-tqw9vx2b	52	26	H120	H120	NNP
cord-297160-tqw9vx2b	52	27	and	and	CC
cord-297160-tqw9vx2b	52	28	IB	IB	NNP
cord-297160-tqw9vx2b	52	29	D274	D274	NNP
cord-297160-tqw9vx2b	52	30	,	,	,
cord-297160-tqw9vx2b	52	31	based	base	VBN
cord-297160-tqw9vx2b	52	32	on	on	IN
cord-297160-tqw9vx2b	52	33	their	-PRON-	PRP$
cord-297160-tqw9vx2b	52	34	reactivity	reactivity	NN
cord-297160-tqw9vx2b	52	35	with	with	IN
cord-297160-tqw9vx2b	52	36	primers	primer	NNS
cord-297160-tqw9vx2b	52	37	specific	specific	JJ
cord-297160-tqw9vx2b	52	38	for	for	IN
cord-297160-tqw9vx2b	52	39	the	the	DT
cord-297160-tqw9vx2b	52	40	various	various	JJ
cord-297160-tqw9vx2b	52	41	strains	strain	NNS
cord-297160-tqw9vx2b	52	42	(	(	-LRB-
cord-297160-tqw9vx2b	52	43	Worthington	Worthington	NNP
cord-297160-tqw9vx2b	52	44	et	et	NNP
cord-297160-tqw9vx2b	52	45	al	al	NNP
cord-297160-tqw9vx2b	52	46	.	.	NNP
cord-297160-tqw9vx2b	52	47	,	,	,
cord-297160-tqw9vx2b	52	48	2008	2008	CD
cord-297160-tqw9vx2b	52	49	)	)	-RRB-
cord-297160-tqw9vx2b	52	50	.	.	.
cord-297160-tqw9vx2b	53	1	Here	here	RB
cord-297160-tqw9vx2b	53	2	,	,	,
cord-297160-tqw9vx2b	53	3	we	-PRON-	PRP
cord-297160-tqw9vx2b	53	4	took	take	VBD
cord-297160-tqw9vx2b	53	5	advantages	advantage	NNS
cord-297160-tqw9vx2b	53	6	of	of	IN
cord-297160-tqw9vx2b	53	7	the	the	DT
cord-297160-tqw9vx2b	53	8	genomic	genomic	JJ
cord-297160-tqw9vx2b	53	9	level	level	NN
cord-297160-tqw9vx2b	53	10	differences	difference	NNS
cord-297160-tqw9vx2b	53	11	between	between	IN
cord-297160-tqw9vx2b	53	12	the	the	DT
cord-297160-tqw9vx2b	53	13	two	two	CD
cord-297160-tqw9vx2b	53	14	IB	IB	NNP
cord-297160-tqw9vx2b	53	15	strains	strain	NNS
cord-297160-tqw9vx2b	53	16	and	and	CC
cord-297160-tqw9vx2b	53	17	describe	describe	VB
cord-297160-tqw9vx2b	53	18	a	a	DT
cord-297160-tqw9vx2b	53	19	PCR	PCR	NNP
cord-297160-tqw9vx2b	53	20	based	base	VBN
cord-297160-tqw9vx2b	53	21	approach	approach	NN
cord-297160-tqw9vx2b	53	22	to	to	TO
cord-297160-tqw9vx2b	53	23	quantify	quantify	VB
cord-297160-tqw9vx2b	53	24	the	the	DT
cord-297160-tqw9vx2b	53	25	titres	titre	NNS
cord-297160-tqw9vx2b	53	26	of	of	IN
cord-297160-tqw9vx2b	53	27	each	each	DT
cord-297160-tqw9vx2b	53	28	IB	IB	NNP
cord-297160-tqw9vx2b	53	29	virus	virus	NN
cord-297160-tqw9vx2b	53	30	strain	strain	NN
cord-297160-tqw9vx2b	53	31	contained	contain	VBN
cord-297160-tqw9vx2b	53	32	in	in	IN
cord-297160-tqw9vx2b	53	33	the	the	DT
cord-297160-tqw9vx2b	53	34	Poulvac	Poulvac	NNP
cord-297160-tqw9vx2b	53	35	IB	IB	NNP
cord-297160-tqw9vx2b	53	36	vaccine	vaccine	NN
cord-297160-tqw9vx2b	53	37	.	.	.
cord-297160-tqw9vx2b	54	1	Lyophilized	lyophilized	JJ
cord-297160-tqw9vx2b	54	2	vials	vial	NNS
cord-297160-tqw9vx2b	54	3	of	of	IN
cord-297160-tqw9vx2b	54	4	a	a	DT
cord-297160-tqw9vx2b	54	5	routine	routine	JJ
cord-297160-tqw9vx2b	54	6	production	production	NN
cord-297160-tqw9vx2b	54	7	batch	batch	NN
cord-297160-tqw9vx2b	54	8	of	of	IN
cord-297160-tqw9vx2b	54	9	Poulvac	Poulvac	NNP
cord-297160-tqw9vx2b	54	10	®	®	.
cord-297160-tqw9vx2b	54	11	IB	IB	NNP
cord-297160-tqw9vx2b	54	12	primer	primer	NN
cord-297160-tqw9vx2b	54	13	were	be	VBD
cord-297160-tqw9vx2b	54	14	used	use	VBN
cord-297160-tqw9vx2b	54	15	.	.	.
cord-297160-tqw9vx2b	55	1	During	during	IN
cord-297160-tqw9vx2b	55	2	the	the	DT
cord-297160-tqw9vx2b	55	3	manufacturing	manufacturing	NN
cord-297160-tqw9vx2b	55	4	process	process	NN
cord-297160-tqw9vx2b	55	5	4	4	CD
cord-297160-tqw9vx2b	55	6	ml	ml	NNS
cord-297160-tqw9vx2b	55	7	of	of	IN
cord-297160-tqw9vx2b	55	8	a	a	DT
cord-297160-tqw9vx2b	55	9	mixture	mixture	NN
cord-297160-tqw9vx2b	55	10	containing	contain	VBG
cord-297160-tqw9vx2b	55	11	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	55	12	fluid	fluid	NN
cord-297160-tqw9vx2b	55	13	with	with	IN
cord-297160-tqw9vx2b	55	14	IB	IB	NNP
cord-297160-tqw9vx2b	55	15	H120	H120	NNP
cord-297160-tqw9vx2b	55	16	,	,	,
cord-297160-tqw9vx2b	55	17	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	55	18	fluid	fluid	NN
cord-297160-tqw9vx2b	55	19	with	with	IN
cord-297160-tqw9vx2b	55	20	IB	IB	NNP
cord-297160-tqw9vx2b	55	21	D274	D274	NNP
cord-297160-tqw9vx2b	55	22	and	and	CC
cord-297160-tqw9vx2b	55	23	a	a	DT
cord-297160-tqw9vx2b	55	24	freeze	freeze	VB
cord-297160-tqw9vx2b	55	25	-	-	HYPH
cord-297160-tqw9vx2b	55	26	dry	dry	JJ
cord-297160-tqw9vx2b	55	27	stabilizer	stabilizer	NN
cord-297160-tqw9vx2b	55	28	was	be	VBD
cord-297160-tqw9vx2b	55	29	filled	fill	VBN
cord-297160-tqw9vx2b	55	30	per	per	IN
cord-297160-tqw9vx2b	55	31	vial	vial	NN
cord-297160-tqw9vx2b	55	32	.	.	.
cord-297160-tqw9vx2b	56	1	The	the	DT
cord-297160-tqw9vx2b	56	2	vials	vial	NNS
cord-297160-tqw9vx2b	56	3	were	be	VBD
cord-297160-tqw9vx2b	56	4	lyophilized	lyophilize	VBN
cord-297160-tqw9vx2b	56	5	according	accord	VBG
cord-297160-tqw9vx2b	56	6	to	to	IN
cord-297160-tqw9vx2b	56	7	routine	routine	JJ
cord-297160-tqw9vx2b	56	8	procedures	procedure	NNS
cord-297160-tqw9vx2b	56	9	and	and	CC
cord-297160-tqw9vx2b	56	10	subsequently	subsequently	RB
cord-297160-tqw9vx2b	56	11	stored	store	VBN
cord-297160-tqw9vx2b	56	12	in	in	IN
cord-297160-tqw9vx2b	56	13	the	the	DT
cord-297160-tqw9vx2b	56	14	dark	dark	NN
cord-297160-tqw9vx2b	56	15	at	at	IN
cord-297160-tqw9vx2b	56	16	5	5	CD
cord-297160-tqw9vx2b	56	17	±	±	CD
cord-297160-tqw9vx2b	56	18	3	3	CD
cord-297160-tqw9vx2b	57	1	•	•	NNP
cord-297160-tqw9vx2b	58	1	C.	C.	NNP
cord-297160-tqw9vx2b	58	2	Titrations	Titrations	NNP
cord-297160-tqw9vx2b	58	3	of	of	IN
cord-297160-tqw9vx2b	58	4	IB	IB	NNP
cord-297160-tqw9vx2b	58	5	virus	virus	NN
cord-297160-tqw9vx2b	58	6	were	be	VBD
cord-297160-tqw9vx2b	58	7	performed	perform	VBN
cord-297160-tqw9vx2b	58	8	according	accord	VBG
cord-297160-tqw9vx2b	58	9	to	to	IN
cord-297160-tqw9vx2b	58	10	standard	standard	JJ
cord-297160-tqw9vx2b	58	11	procedures	procedure	NNS
cord-297160-tqw9vx2b	58	12	as	as	IN
cord-297160-tqw9vx2b	58	13	described	describe	VBN
cord-297160-tqw9vx2b	58	14	by	by	IN
cord-297160-tqw9vx2b	58	15	Doherty	Doherty	NNP
cord-297160-tqw9vx2b	58	16	(	(	-LRB-
cord-297160-tqw9vx2b	58	17	1967	1967	CD
cord-297160-tqw9vx2b	58	18	)	)	-RRB-
cord-297160-tqw9vx2b	58	19	.	.	.
cord-297160-tqw9vx2b	59	1	Specific	specific	JJ
cord-297160-tqw9vx2b	59	2	pathogen	pathogen	NN
cord-297160-tqw9vx2b	59	3	free	free	JJ
cord-297160-tqw9vx2b	59	4	chicken	chicken	NN
cord-297160-tqw9vx2b	59	5	(	(	-LRB-
cord-297160-tqw9vx2b	59	6	SPF	SPF	NNP
cord-297160-tqw9vx2b	59	7	)	)	-RRB-
cord-297160-tqw9vx2b	59	8	eggs	egg	NNS
cord-297160-tqw9vx2b	59	9	were	be	VBD
cord-297160-tqw9vx2b	59	10	obtained	obtain	VBN
cord-297160-tqw9vx2b	59	11	from	from	IN
cord-297160-tqw9vx2b	59	12	ValoBioMedia	valobiomedia	NN
cord-297160-tqw9vx2b	59	13	,	,	,
cord-297160-tqw9vx2b	59	14	Osterholz	Osterholz	NNP
cord-297160-tqw9vx2b	59	15	-	-	HYPH
cord-297160-tqw9vx2b	59	16	Scharmbeck	Scharmbeck	NNP
cord-297160-tqw9vx2b	59	17	,	,	,
cord-297160-tqw9vx2b	59	18	Germany	Germany	NNP
cord-297160-tqw9vx2b	59	19	.	.	.
cord-297160-tqw9vx2b	60	1	Three	three	CD
cord-297160-tqw9vx2b	60	2	vials	vial	NNS
cord-297160-tqw9vx2b	60	3	of	of	IN
cord-297160-tqw9vx2b	60	4	each	each	DT
cord-297160-tqw9vx2b	60	5	vaccine	vaccine	NN
cord-297160-tqw9vx2b	60	6	batch	batch	NN
cord-297160-tqw9vx2b	60	7	were	be	VBD
cord-297160-tqw9vx2b	60	8	tested	test	VBN
cord-297160-tqw9vx2b	60	9	per	per	IN
cord-297160-tqw9vx2b	60	10	titration	titration	NN
cord-297160-tqw9vx2b	60	11	assay	assay	NN
cord-297160-tqw9vx2b	60	12	.	.	.
cord-297160-tqw9vx2b	61	1	The	the	DT
cord-297160-tqw9vx2b	61	2	content	content	NN
cord-297160-tqw9vx2b	61	3	of	of	IN
cord-297160-tqw9vx2b	61	4	each	each	DT
cord-297160-tqw9vx2b	61	5	vial	vial	NN
cord-297160-tqw9vx2b	61	6	of	of	IN
cord-297160-tqw9vx2b	61	7	lyophilized	lyophilized	JJ
cord-297160-tqw9vx2b	61	8	product	product	NN
cord-297160-tqw9vx2b	61	9	was	be	VBD
cord-297160-tqw9vx2b	61	10	reconstituted	reconstitute	VBN
cord-297160-tqw9vx2b	61	11	in	in	IN
cord-297160-tqw9vx2b	61	12	4	4	CD
cord-297160-tqw9vx2b	61	13	ml	ml	NN
cord-297160-tqw9vx2b	61	14	water	water	NN
cord-297160-tqw9vx2b	61	15	for	for	IN
cord-297160-tqw9vx2b	61	16	injection	injection	NN
cord-297160-tqw9vx2b	61	17	.	.	.
cord-297160-tqw9vx2b	62	1	Each	each	DT
cord-297160-tqw9vx2b	62	2	sample	sample	NN
cord-297160-tqw9vx2b	62	3	was	be	VBD
cord-297160-tqw9vx2b	62	4	diluted	dilute	VBN
cord-297160-tqw9vx2b	62	5	further	further	RB
cord-297160-tqw9vx2b	62	6	in	in	IN
cord-297160-tqw9vx2b	62	7	nutrient	nutrient	NN
cord-297160-tqw9vx2b	62	8	broth	broth	NN
cord-297160-tqw9vx2b	63	1	(	(	-LRB-
cord-297160-tqw9vx2b	63	2	Becton	Becton	NNP
cord-297160-tqw9vx2b	63	3	Dickinson	Dickinson	NNP
cord-297160-tqw9vx2b	63	4	,	,	,
cord-297160-tqw9vx2b	63	5	Breda	Breda	NNP
cord-297160-tqw9vx2b	63	6	,	,	,
cord-297160-tqw9vx2b	63	7	The	the	DT
cord-297160-tqw9vx2b	63	8	Netherlands	Netherlands	NNP
cord-297160-tqw9vx2b	63	9	)	)	-RRB-
cord-297160-tqw9vx2b	63	10	containing	contain	VBG
cord-297160-tqw9vx2b	63	11	50	50	CD
cord-297160-tqw9vx2b	63	12	g	g	NN
cord-297160-tqw9vx2b	63	13	/	/	SYM
cord-297160-tqw9vx2b	63	14	ml	ml	NN
cord-297160-tqw9vx2b	63	15	gentamicin	gentamicin	NN
cord-297160-tqw9vx2b	63	16	sulphate	sulphate	NN
cord-297160-tqw9vx2b	63	17	(	(	-LRB-
cord-297160-tqw9vx2b	63	18	Sigma	Sigma	NNP
cord-297160-tqw9vx2b	63	19	-	-	HYPH
cord-297160-tqw9vx2b	63	20	Aldrich	Aldrich	NNP
cord-297160-tqw9vx2b	63	21	Chemie	Chemie	NNP
cord-297160-tqw9vx2b	63	22	BV	BV	NNP
cord-297160-tqw9vx2b	63	23	,	,	,
cord-297160-tqw9vx2b	63	24	Zwijndrecht	Zwijndrecht	NNP
cord-297160-tqw9vx2b	63	25	,	,	,
cord-297160-tqw9vx2b	63	26	The	the	DT
cord-297160-tqw9vx2b	63	27	Netherlands	Netherlands	NNP
cord-297160-tqw9vx2b	63	28	)	)	-RRB-
cord-297160-tqw9vx2b	63	29	,	,	,
cord-297160-tqw9vx2b	63	30	up	up	IN
cord-297160-tqw9vx2b	63	31	to	to	IN
cord-297160-tqw9vx2b	63	32	the	the	DT
cord-297160-tqw9vx2b	63	33	beginning	beginning	NN
cord-297160-tqw9vx2b	63	34	of	of	IN
cord-297160-tqw9vx2b	63	35	the	the	DT
cord-297160-tqw9vx2b	63	36	dilution	dilution	NN
cord-297160-tqw9vx2b	63	37	series	series	NN
cord-297160-tqw9vx2b	63	38	to	to	TO
cord-297160-tqw9vx2b	63	39	be	be	VB
cord-297160-tqw9vx2b	63	40	inoculated	inoculate	VBN
cord-297160-tqw9vx2b	63	41	in	in	IN
cord-297160-tqw9vx2b	63	42	eggs	egg	NNS
cord-297160-tqw9vx2b	63	43	.	.	.
cord-297160-tqw9vx2b	64	1	In	in	IN
cord-297160-tqw9vx2b	64	2	this	this	DT
cord-297160-tqw9vx2b	64	3	series	series	NN
cord-297160-tqw9vx2b	64	4	10-fold	10-fold	CD
cord-297160-tqw9vx2b	64	5	dilution	dilution	NN
cord-297160-tqw9vx2b	64	6	steps	step	NNS
cord-297160-tqw9vx2b	64	7	were	be	VBD
cord-297160-tqw9vx2b	64	8	made	make	VBN
cord-297160-tqw9vx2b	64	9	and	and	CC
cord-297160-tqw9vx2b	64	10	per	per	IN
cord-297160-tqw9vx2b	64	11	dilution	dilution	NN
cord-297160-tqw9vx2b	64	12	6	6	CD
cord-297160-tqw9vx2b	64	13	10	10	CD
cord-297160-tqw9vx2b	64	14	days	day	NNS
cord-297160-tqw9vx2b	64	15	embryonated	embryonate	VBN
cord-297160-tqw9vx2b	64	16	eggs	egg	NNS
cord-297160-tqw9vx2b	64	17	were	be	VBD
cord-297160-tqw9vx2b	64	18	inoculated	inoculate	VBN
cord-297160-tqw9vx2b	64	19	.	.	.
cord-297160-tqw9vx2b	65	1	After	after	IN
cord-297160-tqw9vx2b	65	2	inoculation	inoculation	NN
cord-297160-tqw9vx2b	65	3	the	the	DT
cord-297160-tqw9vx2b	65	4	eggs	egg	NNS
cord-297160-tqw9vx2b	65	5	were	be	VBD
cord-297160-tqw9vx2b	65	6	incubated	incubate	VBN
cord-297160-tqw9vx2b	65	7	at	at	IN
cord-297160-tqw9vx2b	65	8	37	37	CD
cord-297160-tqw9vx2b	65	9	•	•	NN
cord-297160-tqw9vx2b	65	10	C	c	NN
cord-297160-tqw9vx2b	65	11	and	and	CC
cord-297160-tqw9vx2b	65	12	a	a	DT
cord-297160-tqw9vx2b	65	13	relative	relative	JJ
cord-297160-tqw9vx2b	65	14	humidity	humidity	NN
cord-297160-tqw9vx2b	65	15	of	of	IN
cord-297160-tqw9vx2b	65	16	55	55	CD
cord-297160-tqw9vx2b	65	17	%	%	NN
cord-297160-tqw9vx2b	65	18	.	.	.
cord-297160-tqw9vx2b	66	1	After	after	IN
cord-297160-tqw9vx2b	66	2	1	1	CD
cord-297160-tqw9vx2b	66	3	day	day	NN
cord-297160-tqw9vx2b	66	4	incubation	incubation	NN
cord-297160-tqw9vx2b	66	5	eggs	egg	NNS
cord-297160-tqw9vx2b	66	6	with	with	IN
cord-297160-tqw9vx2b	66	7	dead	dead	JJ
cord-297160-tqw9vx2b	66	8	embryos	embryo	NNS
cord-297160-tqw9vx2b	66	9	were	be	VBD
cord-297160-tqw9vx2b	66	10	considered	consider	VBN
cord-297160-tqw9vx2b	66	11	nonspecific	nonspecific	JJ
cord-297160-tqw9vx2b	66	12	deaths	death	NNS
cord-297160-tqw9vx2b	66	13	and	and	CC
cord-297160-tqw9vx2b	66	14	discarded	discard	VBN
cord-297160-tqw9vx2b	66	15	.	.	.
cord-297160-tqw9vx2b	67	1	After	after	IN
cord-297160-tqw9vx2b	67	2	an	an	DT
cord-297160-tqw9vx2b	67	3	incubation	incubation	NN
cord-297160-tqw9vx2b	67	4	period	period	NN
cord-297160-tqw9vx2b	67	5	of	of	IN
cord-297160-tqw9vx2b	67	6	7	7	CD
cord-297160-tqw9vx2b	67	7	days	day	NNS
cord-297160-tqw9vx2b	67	8	the	the	DT
cord-297160-tqw9vx2b	67	9	eggs	egg	NNS
cord-297160-tqw9vx2b	67	10	were	be	VBD
cord-297160-tqw9vx2b	67	11	opened	open	VBN
cord-297160-tqw9vx2b	67	12	and	and	CC
cord-297160-tqw9vx2b	67	13	the	the	DT
cord-297160-tqw9vx2b	67	14	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	67	15	fluid	fluid	NN
cord-297160-tqw9vx2b	67	16	harvested	harvest	VBN
cord-297160-tqw9vx2b	67	17	and	and	CC
cord-297160-tqw9vx2b	67	18	stored	store	VBN
cord-297160-tqw9vx2b	67	19	at	at	IN
cord-297160-tqw9vx2b	68	1	−70	−70	NNP
cord-297160-tqw9vx2b	68	2	•	•	NNP
cord-297160-tqw9vx2b	68	3	C.	c.	NN
cord-297160-tqw9vx2b	69	1	The	the	DT
cord-297160-tqw9vx2b	69	2	embryos	embryo	NNS
cord-297160-tqw9vx2b	69	3	were	be	VBD
cord-297160-tqw9vx2b	69	4	removed	remove	VBN
cord-297160-tqw9vx2b	69	5	and	and	CC
cord-297160-tqw9vx2b	69	6	examined	examine	VBN
cord-297160-tqw9vx2b	69	7	for	for	IN
cord-297160-tqw9vx2b	69	8	the	the	DT
cord-297160-tqw9vx2b	69	9	presence	presence	NN
cord-297160-tqw9vx2b	69	10	of	of	IN
cord-297160-tqw9vx2b	69	11	specific	specific	JJ
cord-297160-tqw9vx2b	69	12	lesions	lesion	NNS
cord-297160-tqw9vx2b	69	13	caused	cause	VBN
cord-297160-tqw9vx2b	69	14	by	by	IN
cord-297160-tqw9vx2b	69	15	the	the	DT
cord-297160-tqw9vx2b	69	16	virus	virus	NN
cord-297160-tqw9vx2b	69	17	,	,	,
cord-297160-tqw9vx2b	69	18	i.e.	i.e.	FW
cord-297160-tqw9vx2b	69	19	dwarfing	dwarfing	NN
cord-297160-tqw9vx2b	69	20	,	,	,
cord-297160-tqw9vx2b	69	21	curling	curling	NN
cord-297160-tqw9vx2b	69	22	and	and	CC
cord-297160-tqw9vx2b	69	23	stunting	stunting	NN
cord-297160-tqw9vx2b	69	24	of	of	IN
cord-297160-tqw9vx2b	69	25	the	the	DT
cord-297160-tqw9vx2b	69	26	embryo	embryo	NN
cord-297160-tqw9vx2b	69	27	.	.	.
cord-297160-tqw9vx2b	70	1	Dead	dead	JJ
cord-297160-tqw9vx2b	70	2	embryos	embryo	NNS
cord-297160-tqw9vx2b	70	3	were	be	VBD
cord-297160-tqw9vx2b	70	4	considered	consider	VBN
cord-297160-tqw9vx2b	70	5	positive	positive	JJ
cord-297160-tqw9vx2b	70	6	for	for	IN
cord-297160-tqw9vx2b	70	7	IBV	IBV	NNP
cord-297160-tqw9vx2b	70	8	.	.	.
cord-297160-tqw9vx2b	71	1	The	the	DT
cord-297160-tqw9vx2b	71	2	titre	titre	NN
cord-297160-tqw9vx2b	71	3	,	,	,
cord-297160-tqw9vx2b	71	4	expressed	express	VBD
cord-297160-tqw9vx2b	71	5	as	as	IN
cord-297160-tqw9vx2b	71	6	the	the	DT
cord-297160-tqw9vx2b	71	7	number	number	NN
cord-297160-tqw9vx2b	71	8	of	of	IN
cord-297160-tqw9vx2b	71	9	EID	EID	NNP
cord-297160-tqw9vx2b	71	10	50	50	CD
cord-297160-tqw9vx2b	71	11	/ml	/ml	:
cord-297160-tqw9vx2b	71	12	was	be	VBD
cord-297160-tqw9vx2b	71	13	calculated	calculate	VBN
cord-297160-tqw9vx2b	71	14	according	accord	VBG
cord-297160-tqw9vx2b	71	15	to	to	IN
cord-297160-tqw9vx2b	71	16	the	the	DT
cord-297160-tqw9vx2b	71	17	method	method	NN
cord-297160-tqw9vx2b	71	18	of	of	IN
cord-297160-tqw9vx2b	71	19	Spearmann	Spearmann	NNP
cord-297160-tqw9vx2b	71	20	-	-	HYPH
cord-297160-tqw9vx2b	71	21	Karber	Karber	NNP
cord-297160-tqw9vx2b	71	22	(	(	-LRB-
cord-297160-tqw9vx2b	71	23	Finney	Finney	NNP
cord-297160-tqw9vx2b	71	24	,	,	,
cord-297160-tqw9vx2b	71	25	1964	1964	CD
cord-297160-tqw9vx2b	71	26	)	)	-RRB-
cord-297160-tqw9vx2b	71	27	.	.	.
cord-297160-tqw9vx2b	72	1	Allantoic	Allantoic	NNP
cord-297160-tqw9vx2b	72	2	fluids	fluid	NNS
cord-297160-tqw9vx2b	72	3	from	from	IN
cord-297160-tqw9vx2b	72	4	the	the	DT
cord-297160-tqw9vx2b	72	5	eggs	egg	NNS
cord-297160-tqw9vx2b	72	6	were	be	VBD
cord-297160-tqw9vx2b	72	7	investigated	investigate	VBN
cord-297160-tqw9vx2b	72	8	for	for	IN
cord-297160-tqw9vx2b	72	9	the	the	DT
cord-297160-tqw9vx2b	72	10	presence	presence	NN
cord-297160-tqw9vx2b	72	11	of	of	IN
cord-297160-tqw9vx2b	72	12	IB	IB	NNP
cord-297160-tqw9vx2b	72	13	virus	virus	NN
cord-297160-tqw9vx2b	72	14	by	by	IN
cord-297160-tqw9vx2b	72	15	reverse	reverse	JJ
cord-297160-tqw9vx2b	72	16	transcriptase	transcriptase	NN
cord-297160-tqw9vx2b	72	17	PCR	pcr	NN
cord-297160-tqw9vx2b	72	18	with	with	IN
cord-297160-tqw9vx2b	72	19	strains	strain	NNS
cord-297160-tqw9vx2b	72	20	specific	specific	JJ
cord-297160-tqw9vx2b	72	21	reverse	reverse	JJ
cord-297160-tqw9vx2b	72	22	transcriptase	transcriptase	NN
cord-297160-tqw9vx2b	72	23	PCR	pcr	NN
cord-297160-tqw9vx2b	72	24	primers	primer	NNS
cord-297160-tqw9vx2b	72	25	for	for	IN
cord-297160-tqw9vx2b	72	26	IB	IB	NNP
cord-297160-tqw9vx2b	72	27	H120	H120	NNP
cord-297160-tqw9vx2b	72	28	and	and	CC
cord-297160-tqw9vx2b	72	29	IB	IB	NNP
cord-297160-tqw9vx2b	72	30	D274	D274	NNP
cord-297160-tqw9vx2b	72	31	.	.	.
cord-297160-tqw9vx2b	73	1	The	the	DT
cord-297160-tqw9vx2b	73	2	RNA	RNA	NNP
cord-297160-tqw9vx2b	73	3	was	be	VBD
cord-297160-tqw9vx2b	73	4	isolated	isolate	VBN
cord-297160-tqw9vx2b	73	5	from	from	IN
cord-297160-tqw9vx2b	73	6	each	each	DT
cord-297160-tqw9vx2b	73	7	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	73	8	fluid	fluid	NN
cord-297160-tqw9vx2b	73	9	samples	sample	NNS
cord-297160-tqw9vx2b	73	10	using	use	VBG
cord-297160-tqw9vx2b	73	11	a	a	DT
cord-297160-tqw9vx2b	73	12	High	high	JJ
cord-297160-tqw9vx2b	73	13	Pure	pure	JJ
cord-297160-tqw9vx2b	73	14	Viral	Viral	NNP
cord-297160-tqw9vx2b	73	15	RNA	RNA	NNP
cord-297160-tqw9vx2b	73	16	kit	kit	NN
cord-297160-tqw9vx2b	73	17	(	(	-LRB-
cord-297160-tqw9vx2b	73	18	Roche	Roche	NNP
cord-297160-tqw9vx2b	73	19	,	,	,
cord-297160-tqw9vx2b	73	20	Almere	Almere	NNP
cord-297160-tqw9vx2b	73	21	,	,	,
cord-297160-tqw9vx2b	73	22	The	the	DT
cord-297160-tqw9vx2b	73	23	Netherlands	Netherlands	NNP
cord-297160-tqw9vx2b	73	24	)	)	-RRB-
cord-297160-tqw9vx2b	73	25	according	accord	VBG
cord-297160-tqw9vx2b	73	26	to	to	IN
cord-297160-tqw9vx2b	73	27	the	the	DT
cord-297160-tqw9vx2b	73	28	instructions	instruction	NNS
cord-297160-tqw9vx2b	73	29	of	of	IN
cord-297160-tqw9vx2b	73	30	the	the	DT
cord-297160-tqw9vx2b	73	31	manufacturer	manufacturer	NN
cord-297160-tqw9vx2b	73	32	.	.	.
cord-297160-tqw9vx2b	74	1	For	for	IN
cord-297160-tqw9vx2b	74	2	PCR	PCR	NNP
cord-297160-tqw9vx2b	74	3	analysis	analysis	NN
cord-297160-tqw9vx2b	74	4	a	a	DT
cord-297160-tqw9vx2b	74	5	PCR	PCR	NNP
cord-297160-tqw9vx2b	74	6	master	master	NN
cord-297160-tqw9vx2b	74	7	mixture	mixture	NN
cord-297160-tqw9vx2b	74	8	was	be	VBD
cord-297160-tqw9vx2b	74	9	prepared	prepared	JJ
cord-297160-tqw9vx2b	74	10	.	.	.
cord-297160-tqw9vx2b	75	1	For	for	IN
cord-297160-tqw9vx2b	75	2	each	each	DT
cord-297160-tqw9vx2b	75	3	sample	sample	NN
cord-297160-tqw9vx2b	75	4	to	to	TO
cord-297160-tqw9vx2b	75	5	be	be	VB
cord-297160-tqw9vx2b	75	6	analysed	analyse	VBN
cord-297160-tqw9vx2b	75	7	2.0	2.0	CD
cord-297160-tqw9vx2b	75	8	l	l	NN
cord-297160-tqw9vx2b	75	9	working	working	NN
cord-297160-tqw9vx2b	75	10	solution	solution	NN
cord-297160-tqw9vx2b	75	11	forward	forward	RB
cord-297160-tqw9vx2b	75	12	primer	primer	NN
cord-297160-tqw9vx2b	75	13	(	(	-LRB-
cord-297160-tqw9vx2b	75	14	100	100	CD
cord-297160-tqw9vx2b	75	15	M	M	NNP
cord-297160-tqw9vx2b	75	16	primer	primer	NN
cord-297160-tqw9vx2b	75	17	)	)	-RRB-
cord-297160-tqw9vx2b	75	18	,	,	,
cord-297160-tqw9vx2b	75	19	2.0	2.0	CD
cord-297160-tqw9vx2b	75	20	l	l	NN
cord-297160-tqw9vx2b	75	21	working	working	NN
cord-297160-tqw9vx2b	75	22	solution	solution	NN
cord-297160-tqw9vx2b	75	23	reverse	reverse	NN
cord-297160-tqw9vx2b	75	24	primer	primer	NN
cord-297160-tqw9vx2b	75	25	(	(	-LRB-
cord-297160-tqw9vx2b	75	26	100	100	CD
cord-297160-tqw9vx2b	75	27	M	M	NNP
cord-297160-tqw9vx2b	75	28	primer	primer	NN
cord-297160-tqw9vx2b	75	29	)	)	-RRB-
cord-297160-tqw9vx2b	75	30	,	,	,
cord-297160-tqw9vx2b	75	31	1.0	1.0	CD
cord-297160-tqw9vx2b	75	32	l	l	NN
cord-297160-tqw9vx2b	75	33	Taq	taq	NN
cord-297160-tqw9vx2b	75	34	-	-	HYPH
cord-297160-tqw9vx2b	75	35	mix	mix	NN
cord-297160-tqw9vx2b	75	36	,	,	,
cord-297160-tqw9vx2b	75	37	25.0	25.0	CD
cord-297160-tqw9vx2b	75	38	l	l	NN
cord-297160-tqw9vx2b	75	39	2×	2×	CD
cord-297160-tqw9vx2b	75	40	buffer	buffer	NN
cord-297160-tqw9vx2b	75	41	,	,	,
cord-297160-tqw9vx2b	75	42	was	be	VBD
cord-297160-tqw9vx2b	75	43	mixed	mix	VBN
cord-297160-tqw9vx2b	75	44	with	with	IN
cord-297160-tqw9vx2b	75	45	15.0	15.0	CD
cord-297160-tqw9vx2b	75	46	l	l	NNP
cord-297160-tqw9vx2b	75	47	PCR	PCR	NNP
cord-297160-tqw9vx2b	75	48	grade	grade	NN
cord-297160-tqw9vx2b	75	49	water	water	NN
cord-297160-tqw9vx2b	75	50	.	.	.
cord-297160-tqw9vx2b	76	1	Taq	taq	NN
cord-297160-tqw9vx2b	76	2	-	-	HYPH
cord-297160-tqw9vx2b	76	3	mix	mix	NN
cord-297160-tqw9vx2b	76	4	and	and	CC
cord-297160-tqw9vx2b	76	5	2×	2×	CD
cord-297160-tqw9vx2b	76	6	buffer	buffer	NN
cord-297160-tqw9vx2b	76	7	had	have	VBD
cord-297160-tqw9vx2b	76	8	been	be	VBN
cord-297160-tqw9vx2b	76	9	obtained	obtain	VBN
cord-297160-tqw9vx2b	76	10	from	from	IN
cord-297160-tqw9vx2b	76	11	the	the	DT
cord-297160-tqw9vx2b	76	12	Super	Super	NNP
cord-297160-tqw9vx2b	76	13	script	script	NN
cord-297160-tqw9vx2b	76	14	One	one	CD
cord-297160-tqw9vx2b	76	15	Step	Step	NNP
cord-297160-tqw9vx2b	76	16	Reverse	Reverse	NNP
cord-297160-tqw9vx2b	76	17	Transcriptase	Transcriptase	NNP
cord-297160-tqw9vx2b	76	18	PCR	pcr	NN
cord-297160-tqw9vx2b	76	19	Kit	kit	NN
cord-297160-tqw9vx2b	76	20	(	(	-LRB-
cord-297160-tqw9vx2b	76	21	Invitrogen	Invitrogen	NNP
cord-297160-tqw9vx2b	76	22	,	,	,
cord-297160-tqw9vx2b	76	23	Bleiswijk	Bleiswijk	NNP
cord-297160-tqw9vx2b	76	24	,	,	,
cord-297160-tqw9vx2b	76	25	The	the	DT
cord-297160-tqw9vx2b	76	26	Netherlands	Netherlands	NNP
cord-297160-tqw9vx2b	76	27	)	)	-RRB-
cord-297160-tqw9vx2b	76	28	.	.	.
cord-297160-tqw9vx2b	77	1	For	for	IN
cord-297160-tqw9vx2b	77	2	one	one	CD
cord-297160-tqw9vx2b	77	3	sample	sample	NN
cord-297160-tqw9vx2b	77	4	45	45	CD
cord-297160-tqw9vx2b	77	5	l	l	NN
cord-297160-tqw9vx2b	77	6	of	of	IN
cord-297160-tqw9vx2b	77	7	this	this	DT
cord-297160-tqw9vx2b	77	8	mixture	mixture	NN
cord-297160-tqw9vx2b	77	9	was	be	VBD
cord-297160-tqw9vx2b	77	10	added	add	VBN
cord-297160-tqw9vx2b	77	11	to	to	IN
cord-297160-tqw9vx2b	77	12	a	a	DT
cord-297160-tqw9vx2b	77	13	PCR	PCR	NNP
cord-297160-tqw9vx2b	77	14	tube	tube	NN
cord-297160-tqw9vx2b	77	15	containing	contain	VBG
cord-297160-tqw9vx2b	77	16	5.0	5.0	CD
cord-297160-tqw9vx2b	77	17	l	l	NN
cord-297160-tqw9vx2b	77	18	of	of	IN
cord-297160-tqw9vx2b	77	19	the	the	DT
cord-297160-tqw9vx2b	77	20	RNA	RNA	NNP
cord-297160-tqw9vx2b	77	21	sample	sample	NN
cord-297160-tqw9vx2b	77	22	.	.	.
cord-297160-tqw9vx2b	78	1	5.0	5.0	CD
cord-297160-tqw9vx2b	78	2	l	l	NN
cord-297160-tqw9vx2b	78	3	of	of	IN
cord-297160-tqw9vx2b	78	4	PCR	PCR	NNP
cord-297160-tqw9vx2b	78	5	grade	grade	NN
cord-297160-tqw9vx2b	78	6	water	water	NN
cord-297160-tqw9vx2b	78	7	was	be	VBD
cord-297160-tqw9vx2b	78	8	used	use	VBN
cord-297160-tqw9vx2b	78	9	instead	instead	RB
cord-297160-tqw9vx2b	78	10	in	in	IN
cord-297160-tqw9vx2b	78	11	case	case	NN
cord-297160-tqw9vx2b	78	12	of	of	IN
cord-297160-tqw9vx2b	78	13	a	a	DT
cord-297160-tqw9vx2b	78	14	negative	negative	JJ
cord-297160-tqw9vx2b	78	15	control	control	NN
cord-297160-tqw9vx2b	78	16	.	.	.
cord-297160-tqw9vx2b	79	1	The	the	DT
cord-297160-tqw9vx2b	79	2	primers	primer	NNS
cord-297160-tqw9vx2b	79	3	for	for	IN
cord-297160-tqw9vx2b	79	4	the	the	DT
cord-297160-tqw9vx2b	79	5	strains	strain	NNS
cord-297160-tqw9vx2b	79	6	IB	IB	NNP
cord-297160-tqw9vx2b	79	7	H120	H120	NNP
cord-297160-tqw9vx2b	79	8	and	and	CC
cord-297160-tqw9vx2b	79	9	IB	IB	NNP
cord-297160-tqw9vx2b	79	10	D274	D274	NNP
cord-297160-tqw9vx2b	79	11	and	and	CC
cord-297160-tqw9vx2b	79	12	the	the	DT
cord-297160-tqw9vx2b	79	13	size	size	NN
cord-297160-tqw9vx2b	79	14	of	of	IN
cord-297160-tqw9vx2b	79	15	the	the	DT
cord-297160-tqw9vx2b	79	16	PCR	PCR	NNP
cord-297160-tqw9vx2b	79	17	products	product	NNS
cord-297160-tqw9vx2b	79	18	are	be	VBP
cord-297160-tqw9vx2b	79	19	indicated	indicate	VBN
cord-297160-tqw9vx2b	79	20	in	in	IN
cord-297160-tqw9vx2b	79	21	Table	Table	NNP
cord-297160-tqw9vx2b	79	22	1	1	CD
cord-297160-tqw9vx2b	79	23	.	.	.
cord-297160-tqw9vx2b	80	1	The	the	DT
cord-297160-tqw9vx2b	80	2	tubes	tube	NNS
cord-297160-tqw9vx2b	80	3	were	be	VBD
cord-297160-tqw9vx2b	80	4	placed	place	VBN
cord-297160-tqw9vx2b	80	5	in	in	IN
cord-297160-tqw9vx2b	80	6	the	the	DT
cord-297160-tqw9vx2b	80	7	PCR	PCR	NNP
cord-297160-tqw9vx2b	80	8	machine	machine	NN
cord-297160-tqw9vx2b	80	9	(	(	-LRB-
cord-297160-tqw9vx2b	80	10	Thermocycler	Thermocycler	NNP
cord-297160-tqw9vx2b	80	11	,	,	,
cord-297160-tqw9vx2b	80	12	MJ	MJ	NNP
cord-297160-tqw9vx2b	80	13	Research	Research	NNP
cord-297160-tqw9vx2b	80	14	PTC-200	PTC-200	NNP
cord-297160-tqw9vx2b	80	15	,	,	,
cord-297160-tqw9vx2b	80	16	Bio	Bio	NNP
cord-297160-tqw9vx2b	80	17	-	-	HYPH
cord-297160-tqw9vx2b	80	18	Rad	Rad	NNP
cord-297160-tqw9vx2b	80	19	,	,	,
cord-297160-tqw9vx2b	80	20	Veenendaal	Veenendaal	NNP
cord-297160-tqw9vx2b	80	21	,	,	,
cord-297160-tqw9vx2b	80	22	The	the	DT
cord-297160-tqw9vx2b	80	23	Netherlands	Netherlands	NNP
cord-297160-tqw9vx2b	80	24	)	)	-RRB-
cord-297160-tqw9vx2b	80	25	and	and	CC
cord-297160-tqw9vx2b	80	26	the	the	DT
cord-297160-tqw9vx2b	80	27	following	following	JJ
cord-297160-tqw9vx2b	80	28	steps	step	NNS
cord-297160-tqw9vx2b	80	29	were	be	VBD
cord-297160-tqw9vx2b	80	30	programmed	program	VBN
cord-297160-tqw9vx2b	80	31	:	:	:
cord-297160-tqw9vx2b	80	32	cDNA	cDNA	NNP
cord-297160-tqw9vx2b	80	33	synthesis	synthesis	NNP
cord-297160-tqw9vx2b	80	34	30	30	CD
cord-297160-tqw9vx2b	80	35	min	min	NN
cord-297160-tqw9vx2b	80	36	at	at	IN
cord-297160-tqw9vx2b	80	37	50	50	CD
cord-297160-tqw9vx2b	80	38	•	•	NNS
cord-297160-tqw9vx2b	80	39	C	c	NN
cord-297160-tqw9vx2b	80	40	,	,	,
cord-297160-tqw9vx2b	80	41	initial	initial	JJ
cord-297160-tqw9vx2b	80	42	denaturation	denaturation	NN
cord-297160-tqw9vx2b	80	43	10	10	CD
cord-297160-tqw9vx2b	80	44	min	min	NN
cord-297160-tqw9vx2b	80	45	at	at	IN
cord-297160-tqw9vx2b	80	46	95	95	CD
cord-297160-tqw9vx2b	81	1	•	•	NNP
cord-297160-tqw9vx2b	82	1	C	C	NNP
cord-297160-tqw9vx2b	82	2	,	,	,
cord-297160-tqw9vx2b	82	3	30	30	CD
cord-297160-tqw9vx2b	82	4	cycles	cycle	NNS
cord-297160-tqw9vx2b	82	5	of	of	IN
cord-297160-tqw9vx2b	82	6	30	30	CD
cord-297160-tqw9vx2b	82	7	s	s	NN
cord-297160-tqw9vx2b	82	8	at	at	IN
cord-297160-tqw9vx2b	82	9	95	95	CD
cord-297160-tqw9vx2b	83	1	•	•	NNP
cord-297160-tqw9vx2b	84	1	C	C	NNP
cord-297160-tqw9vx2b	84	2	,	,	,
cord-297160-tqw9vx2b	84	3	30	30	CD
cord-297160-tqw9vx2b	84	4	s	s	NN
cord-297160-tqw9vx2b	84	5	at	at	IN
cord-297160-tqw9vx2b	84	6	50	50	CD
cord-297160-tqw9vx2b	84	7	•	•	NN
cord-297160-tqw9vx2b	84	8	C	c	NN
cord-297160-tqw9vx2b	84	9	,	,	,
cord-297160-tqw9vx2b	85	1	45	45	CD
cord-297160-tqw9vx2b	85	2	s	s	NN
cord-297160-tqw9vx2b	85	3	at	at	IN
cord-297160-tqw9vx2b	85	4	72	72	CD
cord-297160-tqw9vx2b	86	1	•	•	NNP
cord-297160-tqw9vx2b	87	1	C.	C.	NNP
cord-297160-tqw9vx2b	87	2	Final	Final	NNP
cord-297160-tqw9vx2b	87	3	extension	extension	NN
cord-297160-tqw9vx2b	87	4	was	be	VBD
cord-297160-tqw9vx2b	87	5	7	7	CD
cord-297160-tqw9vx2b	87	6	min	min	NN
cord-297160-tqw9vx2b	87	7	at	at	IN
cord-297160-tqw9vx2b	87	8	72	72	CD
cord-297160-tqw9vx2b	88	1	•	•	NNP
cord-297160-tqw9vx2b	89	1	C	C	NNP
cord-297160-tqw9vx2b	89	2	and	and	CC
cord-297160-tqw9vx2b	89	3	reaction	reaction	NN
cord-297160-tqw9vx2b	89	4	stop	stop	NN
cord-297160-tqw9vx2b	89	5	5	5	CD
cord-297160-tqw9vx2b	89	6	min	min	NN
cord-297160-tqw9vx2b	89	7	at	at	IN
cord-297160-tqw9vx2b	89	8	4	4	CD
cord-297160-tqw9vx2b	90	1	•	•	NNP
cord-297160-tqw9vx2b	91	1	C.	C.	NNP
cord-297160-tqw9vx2b	92	1	The	the	DT
cord-297160-tqw9vx2b	92	2	formation	formation	NN
cord-297160-tqw9vx2b	92	3	of	of	IN
cord-297160-tqw9vx2b	92	4	PCR	PCR	NNP
cord-297160-tqw9vx2b	92	5	product	product	NN
cord-297160-tqw9vx2b	92	6	was	be	VBD
cord-297160-tqw9vx2b	92	7	assessed	assess	VBN
cord-297160-tqw9vx2b	92	8	by	by	IN
cord-297160-tqw9vx2b	92	9	agarose	agarose	JJ
cord-297160-tqw9vx2b	92	10	gel	gel	NN
cord-297160-tqw9vx2b	92	11	electrophoresis	electrophoresis	NN
cord-297160-tqw9vx2b	92	12	.	.	.
cord-297160-tqw9vx2b	93	1	First	first	RB
cord-297160-tqw9vx2b	93	2	the	the	DT
cord-297160-tqw9vx2b	93	3	gel	gel	NN
cord-297160-tqw9vx2b	93	4	was	be	VBD
cord-297160-tqw9vx2b	93	5	prepared	prepare	VBN
cord-297160-tqw9vx2b	93	6	.	.	.
cord-297160-tqw9vx2b	94	1	TBE	TBE	NNP
cord-297160-tqw9vx2b	94	2	buffer	buffer	NN
cord-297160-tqw9vx2b	94	3	was	be	VBD
cord-297160-tqw9vx2b	94	4	prepared	prepare	VBN
cord-297160-tqw9vx2b	94	5	by	by	IN
cord-297160-tqw9vx2b	94	6	10-fold	10-fold	JJ
cord-297160-tqw9vx2b	94	7	dilution	dilution	NN
cord-297160-tqw9vx2b	94	8	of	of	IN
cord-297160-tqw9vx2b	94	9	a	a	DT
cord-297160-tqw9vx2b	94	10	solution	solution	NN
cord-297160-tqw9vx2b	94	11	with	with	IN
cord-297160-tqw9vx2b	94	12	1.0	1.0	CD
cord-297160-tqw9vx2b	94	13	M	M	NNP
cord-297160-tqw9vx2b	94	14	Tris	Tris	NNP
cord-297160-tqw9vx2b	94	15	,	,	,
cord-297160-tqw9vx2b	94	16	0.9	0.9	CD
cord-297160-tqw9vx2b	94	17	M	m	NN
cord-297160-tqw9vx2b	94	18	boric	boric	NN
cord-297160-tqw9vx2b	94	19	acid	acid	NN
cord-297160-tqw9vx2b	94	20	,	,	,
cord-297160-tqw9vx2b	94	21	0.01	0.01	CD
cord-297160-tqw9vx2b	94	22	M	M	NNP
cord-297160-tqw9vx2b	94	23	EDTA	EDTA	NNP
cord-297160-tqw9vx2b	94	24	in	in	IN
cord-297160-tqw9vx2b	94	25	water	water	NN
cord-297160-tqw9vx2b	94	26	.	.	.
cord-297160-tqw9vx2b	95	1	Agarose	Agarose	NNP
cord-297160-tqw9vx2b	95	2	was	be	VBD
cord-297160-tqw9vx2b	95	3	added	add	VBN
cord-297160-tqw9vx2b	95	4	to	to	IN
cord-297160-tqw9vx2b	95	5	a	a	DT
cord-297160-tqw9vx2b	95	6	concentration	concentration	NN
cord-297160-tqw9vx2b	95	7	of	of	IN
cord-297160-tqw9vx2b	95	8	2	2	CD
cord-297160-tqw9vx2b	95	9	%	%	NN
cord-297160-tqw9vx2b	95	10	to	to	IN
cord-297160-tqw9vx2b	95	11	the	the	DT
cord-297160-tqw9vx2b	95	12	TBE	TBE	NNP
cord-297160-tqw9vx2b	95	13	buffer	buffer	NN
cord-297160-tqw9vx2b	95	14	and	and	CC
cord-297160-tqw9vx2b	95	15	ethydium	ethydium	NN
cord-297160-tqw9vx2b	95	16	bromide	bromide	NN
cord-297160-tqw9vx2b	95	17	to	to	IN
cord-297160-tqw9vx2b	95	18	a	a	DT
cord-297160-tqw9vx2b	95	19	final	final	JJ
cord-297160-tqw9vx2b	95	20	concentration	concentration	NN
cord-297160-tqw9vx2b	95	21	of	of	IN
cord-297160-tqw9vx2b	95	22	0.5	0.5	CD
cord-297160-tqw9vx2b	95	23	g	g	NN
cord-297160-tqw9vx2b	95	24	per	per	IN
cord-297160-tqw9vx2b	95	25	ml	ml	NN
cord-297160-tqw9vx2b	95	26	.	.	.
cord-297160-tqw9vx2b	96	1	The	the	DT
cord-297160-tqw9vx2b	96	2	gel	gel	NN
cord-297160-tqw9vx2b	96	3	was	be	VBD
cord-297160-tqw9vx2b	96	4	allowed	allow	VBN
cord-297160-tqw9vx2b	96	5	to	to	TO
cord-297160-tqw9vx2b	96	6	solidify	solidify	VB
cord-297160-tqw9vx2b	96	7	and	and	CC
cord-297160-tqw9vx2b	96	8	used	use	VBN
cord-297160-tqw9vx2b	96	9	for	for	IN
cord-297160-tqw9vx2b	96	10	electrophoresis	electrophoresis	NN
cord-297160-tqw9vx2b	96	11	of	of	IN
cord-297160-tqw9vx2b	96	12	the	the	DT
cord-297160-tqw9vx2b	96	13	PCR	PCR	NNP
cord-297160-tqw9vx2b	96	14	samples	sample	NNS
cord-297160-tqw9vx2b	96	15	.	.	.
cord-297160-tqw9vx2b	97	1	The	the	DT
cord-297160-tqw9vx2b	97	2	samples	sample	NNS
cord-297160-tqw9vx2b	97	3	were	be	VBD
cord-297160-tqw9vx2b	97	4	allowed	allow	VBN
cord-297160-tqw9vx2b	97	5	to	to	TO
cord-297160-tqw9vx2b	97	6	go	go	VB
cord-297160-tqw9vx2b	97	7	through	through	IN
cord-297160-tqw9vx2b	97	8	the	the	DT
cord-297160-tqw9vx2b	97	9	gel	gel	NN
cord-297160-tqw9vx2b	97	10	for	for	IN
cord-297160-tqw9vx2b	97	11	2	2	CD
cord-297160-tqw9vx2b	97	12	h	h	NN
cord-297160-tqw9vx2b	97	13	at	at	IN
cord-297160-tqw9vx2b	97	14	70	70	CD
cord-297160-tqw9vx2b	98	1	V.	V.	NNP
cord-297160-tqw9vx2b	98	2	A	a	DT
cord-297160-tqw9vx2b	98	3	basepair	basepair	NN
cord-297160-tqw9vx2b	98	4	ladder	ladder	NN
cord-297160-tqw9vx2b	98	5	was	be	VBD
cord-297160-tqw9vx2b	98	6	used	use	VBN
cord-297160-tqw9vx2b	98	7	to	to	TO
cord-297160-tqw9vx2b	98	8	determine	determine	VB
cord-297160-tqw9vx2b	98	9	the	the	DT
cord-297160-tqw9vx2b	98	10	size	size	NN
cord-297160-tqw9vx2b	98	11	of	of	IN
cord-297160-tqw9vx2b	98	12	the	the	DT
cord-297160-tqw9vx2b	98	13	bands	band	NNS
cord-297160-tqw9vx2b	98	14	in	in	IN
cord-297160-tqw9vx2b	98	15	the	the	DT
cord-297160-tqw9vx2b	98	16	agarose	agarose	JJ
cord-297160-tqw9vx2b	98	17	gel	gel	NN
cord-297160-tqw9vx2b	98	18	.	.	.
cord-297160-tqw9vx2b	99	1	Gel	gel	NN
cord-297160-tqw9vx2b	99	2	electrophoresis	electrophoresis	NN
cord-297160-tqw9vx2b	99	3	was	be	VBD
cord-297160-tqw9vx2b	99	4	stopped	stop	VBN
cord-297160-tqw9vx2b	99	5	when	when	WRB
cord-297160-tqw9vx2b	99	6	separation	separation	NN
cord-297160-tqw9vx2b	99	7	of	of	IN
cord-297160-tqw9vx2b	99	8	the	the	DT
cord-297160-tqw9vx2b	99	9	different	different	JJ
cord-297160-tqw9vx2b	99	10	bands	band	NNS
cord-297160-tqw9vx2b	99	11	in	in	IN
cord-297160-tqw9vx2b	99	12	the	the	DT
cord-297160-tqw9vx2b	99	13	base	base	JJ
cord-297160-tqw9vx2b	99	14	pair	pair	NN
cord-297160-tqw9vx2b	99	15	ladder	ladder	NN
cord-297160-tqw9vx2b	99	16	was	be	VBD
cord-297160-tqw9vx2b	99	17	satisfactory	satisfactory	JJ
cord-297160-tqw9vx2b	99	18	.	.	.
cord-297160-tqw9vx2b	100	1	Bands	band	NNS
cord-297160-tqw9vx2b	100	2	were	be	VBD
cord-297160-tqw9vx2b	100	3	made	make	VBN
cord-297160-tqw9vx2b	100	4	visible	visible	JJ
cord-297160-tqw9vx2b	100	5	by	by	IN
cord-297160-tqw9vx2b	100	6	ultraviolet	ultraviolet	JJ
cord-297160-tqw9vx2b	100	7	illumination	illumination	NN
cord-297160-tqw9vx2b	100	8	.	.	.
cord-297160-tqw9vx2b	101	1	In	in	IN
cord-297160-tqw9vx2b	101	2	a	a	DT
cord-297160-tqw9vx2b	101	3	first	first	JJ
cord-297160-tqw9vx2b	101	4	experiment	experiment	NN
cord-297160-tqw9vx2b	101	5	,	,	,
cord-297160-tqw9vx2b	101	6	a	a	DT
cord-297160-tqw9vx2b	101	7	dilution	dilution	NN
cord-297160-tqw9vx2b	101	8	series	series	NN
cord-297160-tqw9vx2b	101	9	of	of	IN
cord-297160-tqw9vx2b	101	10	a	a	DT
cord-297160-tqw9vx2b	101	11	batch	batch	NN
cord-297160-tqw9vx2b	101	12	of	of	IN
cord-297160-tqw9vx2b	101	13	Poulvac	Poulvac	NNP
cord-297160-tqw9vx2b	101	14	®	®	.
cord-297160-tqw9vx2b	101	15	IB	IB	NNP
cord-297160-tqw9vx2b	101	16	primer	primer	NN
cord-297160-tqw9vx2b	101	17	was	be	VBD
cord-297160-tqw9vx2b	101	18	prepared	prepare	VBN
cord-297160-tqw9vx2b	101	19	according	accord	VBG
cord-297160-tqw9vx2b	101	20	to	to	IN
cord-297160-tqw9vx2b	101	21	routine	routine	JJ
cord-297160-tqw9vx2b	101	22	production	production	NN
cord-297160-tqw9vx2b	101	23	QC	QC	NNP
cord-297160-tqw9vx2b	101	24	procedures	procedure	NNS
cord-297160-tqw9vx2b	101	25	.	.	.
cord-297160-tqw9vx2b	102	1	The	the	DT
cord-297160-tqw9vx2b	102	2	dilution	dilution	NN
cord-297160-tqw9vx2b	102	3	series	series	NN
cord-297160-tqw9vx2b	102	4	ranged	range	VBD
cord-297160-tqw9vx2b	102	5	from	from	IN
cord-297160-tqw9vx2b	102	6	10	10	CD
cord-297160-tqw9vx2b	102	7	−4	−4	CD
cord-297160-tqw9vx2b	102	8	to	to	IN
cord-297160-tqw9vx2b	102	9	10	10	CD
cord-297160-tqw9vx2b	102	10	−9	−9	NN
cord-297160-tqw9vx2b	102	11	.	.	.
cord-297160-tqw9vx2b	103	1	All	all	DT
cord-297160-tqw9vx2b	103	2	dilutions	dilution	NNS
cord-297160-tqw9vx2b	103	3	were	be	VBD
cord-297160-tqw9vx2b	103	4	tested	test	VBN
cord-297160-tqw9vx2b	103	5	for	for	IN
cord-297160-tqw9vx2b	103	6	presence	presence	NN
cord-297160-tqw9vx2b	103	7	of	of	IN
cord-297160-tqw9vx2b	103	8	IB	IB	NNP
cord-297160-tqw9vx2b	103	9	H120	H120	NNP
cord-297160-tqw9vx2b	103	10	and	and	CC
cord-297160-tqw9vx2b	103	11	IB	IB	NNP
cord-297160-tqw9vx2b	103	12	D274	D274	VBN
cord-297160-tqw9vx2b	103	13	by	by	IN
cord-297160-tqw9vx2b	103	14	reverse	reverse	NN
cord-297160-tqw9vx2b	103	15	transciptase	transciptase	NNP
cord-297160-tqw9vx2b	103	16	PCR	PCR	NNP
cord-297160-tqw9vx2b	103	17	.	.	.
cord-297160-tqw9vx2b	104	1	None	none	NN
cord-297160-tqw9vx2b	104	2	of	of	IN
cord-297160-tqw9vx2b	104	3	the	the	DT
cord-297160-tqw9vx2b	104	4	dilutions	dilution	NNS
cord-297160-tqw9vx2b	104	5	was	be	VBD
cord-297160-tqw9vx2b	104	6	positive	positive	JJ
cord-297160-tqw9vx2b	104	7	for	for	IN
cord-297160-tqw9vx2b	104	8	IB	IB	NNP
cord-297160-tqw9vx2b	104	9	by	by	IN
cord-297160-tqw9vx2b	104	10	PCR	PCR	NNP
cord-297160-tqw9vx2b	104	11	,	,	,
cord-297160-tqw9vx2b	104	12	see	see	VB
cord-297160-tqw9vx2b	104	13	Table	table	NN
cord-297160-tqw9vx2b	104	14	2	2	CD
cord-297160-tqw9vx2b	104	15	.	.	.
cord-297160-tqw9vx2b	105	1	Apparently	apparently	RB
cord-297160-tqw9vx2b	105	2	,	,	,
cord-297160-tqw9vx2b	105	3	the	the	DT
cord-297160-tqw9vx2b	105	4	quantities	quantity	NNS
cord-297160-tqw9vx2b	105	5	of	of	IN
cord-297160-tqw9vx2b	105	6	virus	virus	NN
cord-297160-tqw9vx2b	105	7	in	in	IN
cord-297160-tqw9vx2b	105	8	the	the	DT
cord-297160-tqw9vx2b	105	9	dilutions	dilution	NNS
cord-297160-tqw9vx2b	105	10	series	series	NN
cord-297160-tqw9vx2b	105	11	were	be	VBD
cord-297160-tqw9vx2b	105	12	below	below	IN
cord-297160-tqw9vx2b	105	13	the	the	DT
cord-297160-tqw9vx2b	105	14	detection	detection	NN
cord-297160-tqw9vx2b	105	15	level	level	NN
cord-297160-tqw9vx2b	105	16	.	.	.
cord-297160-tqw9vx2b	106	1	The	the	DT
cord-297160-tqw9vx2b	106	2	dilution	dilution	NN
cord-297160-tqw9vx2b	106	3	series	series	NN
cord-297160-tqw9vx2b	106	4	was	be	VBD
cord-297160-tqw9vx2b	106	5	inoculated	inoculate	VBN
cord-297160-tqw9vx2b	106	6	in	in	IN
cord-297160-tqw9vx2b	106	7	embryonated	embryonated	JJ
cord-297160-tqw9vx2b	106	8	SPF	SPF	NNP
cord-297160-tqw9vx2b	106	9	chicken	chicken	NN
cord-297160-tqw9vx2b	106	10	eggs	egg	NNS
cord-297160-tqw9vx2b	106	11	,	,	,
cord-297160-tqw9vx2b	106	12	6	6	CD
cord-297160-tqw9vx2b	106	13	eggs	egg	NNS
cord-297160-tqw9vx2b	106	14	per	per	IN
cord-297160-tqw9vx2b	106	15	dilution	dilution	NN
cord-297160-tqw9vx2b	106	16	.	.	.
cord-297160-tqw9vx2b	107	1	After	after	IN
cord-297160-tqw9vx2b	107	2	the	the	DT
cord-297160-tqw9vx2b	107	3	routine	routine	JJ
cord-297160-tqw9vx2b	107	4	incubation	incubation	NN
cord-297160-tqw9vx2b	107	5	period	period	NN
cord-297160-tqw9vx2b	107	6	samples	sample	NNS
cord-297160-tqw9vx2b	107	7	of	of	IN
cord-297160-tqw9vx2b	107	8	the	the	DT
cord-297160-tqw9vx2b	107	9	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	107	10	fluids	fluid	NNS
cord-297160-tqw9vx2b	107	11	were	be	VBD
cord-297160-tqw9vx2b	107	12	tested	test	VBN
cord-297160-tqw9vx2b	107	13	also	also	RB
cord-297160-tqw9vx2b	107	14	by	by	IN
cord-297160-tqw9vx2b	107	15	PCR	PCR	NNP
cord-297160-tqw9vx2b	107	16	.	.	.
cord-297160-tqw9vx2b	108	1	It	-PRON-	PRP
cord-297160-tqw9vx2b	108	2	now	now	RB
cord-297160-tqw9vx2b	108	3	appeared	appear	VBD
cord-297160-tqw9vx2b	108	4	that	that	IN
cord-297160-tqw9vx2b	108	5	after	after	IN
cord-297160-tqw9vx2b	108	6	the	the	DT
cord-297160-tqw9vx2b	108	7	egg	egg	NN
cord-297160-tqw9vx2b	108	8	passage	passage	NN
cord-297160-tqw9vx2b	108	9	dilutions	dilution	NNS
cord-297160-tqw9vx2b	108	10	up	up	IN
cord-297160-tqw9vx2b	108	11	to	to	TO
cord-297160-tqw9vx2b	108	12	10	10	CD
cord-297160-tqw9vx2b	108	13	−6	−6	NNS
cord-297160-tqw9vx2b	108	14	were	be	VBD
cord-297160-tqw9vx2b	108	15	positive	positive	JJ
cord-297160-tqw9vx2b	108	16	by	by	IN
cord-297160-tqw9vx2b	108	17	PCR	PCR	NNP
cord-297160-tqw9vx2b	108	18	for	for	IN
cord-297160-tqw9vx2b	108	19	IB	IB	NNP
cord-297160-tqw9vx2b	108	20	D274	D274	NNP
cord-297160-tqw9vx2b	108	21	and	and	CC
cord-297160-tqw9vx2b	108	22	dilutions	dilution	NNS
cord-297160-tqw9vx2b	109	1	up	up	IN
cord-297160-tqw9vx2b	109	2	to	to	TO
cord-297160-tqw9vx2b	109	3	10	10	CD
cord-297160-tqw9vx2b	109	4	−7	−7	NNP
cord-297160-tqw9vx2b	109	5	were	be	VBD
cord-297160-tqw9vx2b	109	6	positive	positive	JJ
cord-297160-tqw9vx2b	109	7	by	by	IN
cord-297160-tqw9vx2b	109	8	PCR	PCR	NNP
cord-297160-tqw9vx2b	109	9	for	for	IN
cord-297160-tqw9vx2b	109	10	IB	IB	NNP
cord-297160-tqw9vx2b	109	11	H120	H120	NNP
cord-297160-tqw9vx2b	109	12	,	,	,
cord-297160-tqw9vx2b	109	13	see	see	VB
cord-297160-tqw9vx2b	109	14	Table	table	NN
cord-297160-tqw9vx2b	109	15	2	2	CD
cord-297160-tqw9vx2b	109	16	.	.	.
cord-297160-tqw9vx2b	110	1	The	the	DT
cord-297160-tqw9vx2b	110	2	embryos	embryo	NNS
cord-297160-tqw9vx2b	110	3	were	be	VBD
cord-297160-tqw9vx2b	110	4	also	also	RB
cord-297160-tqw9vx2b	110	5	examined	examine	VBN
cord-297160-tqw9vx2b	110	6	visually	visually	RB
cord-297160-tqw9vx2b	110	7	,	,	,
cord-297160-tqw9vx2b	110	8	and	and	CC
cord-297160-tqw9vx2b	110	9	the	the	DT
cord-297160-tqw9vx2b	110	10	results	result	NNS
cord-297160-tqw9vx2b	110	11	show	show	VBP
cord-297160-tqw9vx2b	110	12	that	that	IN
cord-297160-tqw9vx2b	110	13	at	at	IN
cord-297160-tqw9vx2b	110	14	a	a	DT
cord-297160-tqw9vx2b	110	15	dilution	dilution	NN
cord-297160-tqw9vx2b	110	16	of	of	IN
cord-297160-tqw9vx2b	110	17	10	10	CD
cord-297160-tqw9vx2b	110	18	−6	−6	NNS
cord-297160-tqw9vx2b	110	19	all	all	DT
cord-297160-tqw9vx2b	110	20	(	(	-LRB-
cord-297160-tqw9vx2b	110	21	5	5	CD
cord-297160-tqw9vx2b	110	22	of	of	IN
cord-297160-tqw9vx2b	110	23	5	5	CD
cord-297160-tqw9vx2b	110	24	)	)	-RRB-
cord-297160-tqw9vx2b	111	1	embryos	embryo	NNS
cord-297160-tqw9vx2b	111	2	were	be	VBD
cord-297160-tqw9vx2b	111	3	affected	affect	VBN
cord-297160-tqw9vx2b	111	4	by	by	IN
cord-297160-tqw9vx2b	111	5	the	the	DT
cord-297160-tqw9vx2b	111	6	virus	virus	NN
cord-297160-tqw9vx2b	111	7	.	.	.
cord-297160-tqw9vx2b	112	1	At	at	IN
cord-297160-tqw9vx2b	112	2	a	a	DT
cord-297160-tqw9vx2b	112	3	dilution	dilution	NN
cord-297160-tqw9vx2b	112	4	of	of	IN
cord-297160-tqw9vx2b	112	5	10	10	CD
cord-297160-tqw9vx2b	112	6	−7	−7	CD
cord-297160-tqw9vx2b	112	7	one	one	CD
cord-297160-tqw9vx2b	112	8	of	of	IN
cord-297160-tqw9vx2b	112	9	5	5	CD
cord-297160-tqw9vx2b	112	10	embryos	embryo	NNS
cord-297160-tqw9vx2b	112	11	was	be	VBD
cord-297160-tqw9vx2b	112	12	affected	affect	VBN
cord-297160-tqw9vx2b	112	13	.	.	.
cord-297160-tqw9vx2b	113	1	The	the	DT
cord-297160-tqw9vx2b	113	2	only	only	JJ
cord-297160-tqw9vx2b	113	3	possible	possible	JJ
cord-297160-tqw9vx2b	113	4	explanation	explanation	NN
cord-297160-tqw9vx2b	113	5	for	for	IN
cord-297160-tqw9vx2b	113	6	the	the	DT
cord-297160-tqw9vx2b	113	7	presence	presence	NN
cord-297160-tqw9vx2b	113	8	of	of	IN
cord-297160-tqw9vx2b	113	9	IB	IB	NNP
cord-297160-tqw9vx2b	113	10	H120	H120	NNP
cord-297160-tqw9vx2b	113	11	and	and	CC
cord-297160-tqw9vx2b	113	12	IB	IB	NNP
cord-297160-tqw9vx2b	113	13	D274	D274	NNP
cord-297160-tqw9vx2b	113	14	in	in	IN
cord-297160-tqw9vx2b	113	15	the	the	DT
cord-297160-tqw9vx2b	113	16	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	113	17	fluids	fluid	NNS
cord-297160-tqw9vx2b	113	18	was	be	VBD
cord-297160-tqw9vx2b	113	19	that	that	IN
cord-297160-tqw9vx2b	113	20	the	the	DT
cord-297160-tqw9vx2b	113	21	viruses	virus	NNS
cord-297160-tqw9vx2b	113	22	had	have	VBD
cord-297160-tqw9vx2b	113	23	been	be	VBN
cord-297160-tqw9vx2b	113	24	able	able	JJ
cord-297160-tqw9vx2b	113	25	to	to	TO
cord-297160-tqw9vx2b	113	26	cause	cause	VB
cord-297160-tqw9vx2b	113	27	a	a	DT
cord-297160-tqw9vx2b	113	28	productive	productive	JJ
cord-297160-tqw9vx2b	113	29	infection	infection	NN
cord-297160-tqw9vx2b	113	30	in	in	IN
cord-297160-tqw9vx2b	113	31	the	the	DT
cord-297160-tqw9vx2b	113	32	eggs	egg	NNS
cord-297160-tqw9vx2b	113	33	.	.	.
cord-297160-tqw9vx2b	114	1	It	-PRON-	PRP
cord-297160-tqw9vx2b	114	2	is	be	VBZ
cord-297160-tqw9vx2b	114	3	concluded	conclude	VBN
cord-297160-tqw9vx2b	114	4	that	that	IN
cord-297160-tqw9vx2b	114	5	in	in	IN
cord-297160-tqw9vx2b	114	6	this	this	DT
cord-297160-tqw9vx2b	114	7	test	test	NN
cord-297160-tqw9vx2b	114	8	live	live	JJ
cord-297160-tqw9vx2b	114	9	virus	virus	NN
cord-297160-tqw9vx2b	114	10	titres	titre	NNS
cord-297160-tqw9vx2b	114	11	were	be	VBD
cord-297160-tqw9vx2b	114	12	determined	determine	VBN
cord-297160-tqw9vx2b	114	13	.	.	.
cord-297160-tqw9vx2b	115	1	In	in	IN
cord-297160-tqw9vx2b	115	2	a	a	DT
cord-297160-tqw9vx2b	115	3	second	second	JJ
cord-297160-tqw9vx2b	115	4	experiment	experiment	NN
cord-297160-tqw9vx2b	115	5	a	a	DT
cord-297160-tqw9vx2b	115	6	routine	routine	JJ
cord-297160-tqw9vx2b	115	7	titration	titration	NN
cord-297160-tqw9vx2b	115	8	on	on	IN
cord-297160-tqw9vx2b	115	9	Poulvac	Poulvac	NNP
cord-297160-tqw9vx2b	115	10	®	®	.
cord-297160-tqw9vx2b	115	11	IB	IB	NNP
cord-297160-tqw9vx2b	115	12	primer	primer	NN
cord-297160-tqw9vx2b	115	13	was	be	VBD
cord-297160-tqw9vx2b	115	14	performed	perform	VBN
cord-297160-tqw9vx2b	115	15	.	.	.
cord-297160-tqw9vx2b	116	1	This	this	DT
cord-297160-tqw9vx2b	116	2	titration	titration	NN
cord-297160-tqw9vx2b	116	3	was	be	VBD
cord-297160-tqw9vx2b	116	4	performed	perform	VBN
cord-297160-tqw9vx2b	116	5	in	in	IN
cord-297160-tqw9vx2b	116	6	triplicate	triplicate	NN
cord-297160-tqw9vx2b	116	7	.	.	.
cord-297160-tqw9vx2b	117	1	After	after	IN
cord-297160-tqw9vx2b	117	2	the	the	DT
cord-297160-tqw9vx2b	117	3	incubation	incubation	NN
cord-297160-tqw9vx2b	117	4	period	period	NN
cord-297160-tqw9vx2b	117	5	the	the	DT
cord-297160-tqw9vx2b	117	6	eggs	egg	NNS
cord-297160-tqw9vx2b	117	7	were	be	VBD
cord-297160-tqw9vx2b	117	8	opened	open	VBN
cord-297160-tqw9vx2b	117	9	and	and	CC
cord-297160-tqw9vx2b	117	10	the	the	DT
cord-297160-tqw9vx2b	117	11	embryos	embryo	NNS
cord-297160-tqw9vx2b	117	12	were	be	VBD
cord-297160-tqw9vx2b	117	13	examined	examine	VBN
cord-297160-tqw9vx2b	117	14	visually	visually	RB
cord-297160-tqw9vx2b	117	15	.	.	.
cord-297160-tqw9vx2b	118	1	Of	of	IN
cord-297160-tqw9vx2b	118	2	each	each	DT
cord-297160-tqw9vx2b	118	3	triplicate	triplicate	NN
cord-297160-tqw9vx2b	118	4	a	a	DT
cord-297160-tqw9vx2b	118	5	titre	titre	NN
cord-297160-tqw9vx2b	118	6	per	per	IN
cord-297160-tqw9vx2b	118	7	vial	vial	NN
cord-297160-tqw9vx2b	118	8	was	be	VBD
cord-297160-tqw9vx2b	118	9	calculated	calculate	VBN
cord-297160-tqw9vx2b	118	10	(	(	-LRB-
cord-297160-tqw9vx2b	118	11	see	see	VB
cord-297160-tqw9vx2b	118	12	bottom	bottom	JJ
cord-297160-tqw9vx2b	118	13	Table	Table	NNP
cord-297160-tqw9vx2b	118	14	3	3	CD
cord-297160-tqw9vx2b	118	15	under	under	IN
cord-297160-tqw9vx2b	118	16	Visual	Visual	NNP
cord-297160-tqw9vx2b	118	17	)	)	-RRB-
cord-297160-tqw9vx2b	118	18	and	and	CC
cord-297160-tqw9vx2b	118	19	from	from	IN
cord-297160-tqw9vx2b	118	20	the	the	DT
cord-297160-tqw9vx2b	118	21	3	3	CD
cord-297160-tqw9vx2b	118	22	scores	score	NNS
cord-297160-tqw9vx2b	118	23	obtained	obtain	VBN
cord-297160-tqw9vx2b	118	24	,	,	,
cord-297160-tqw9vx2b	118	25	i.e.	i.e.	FW
cord-297160-tqw9vx2b	118	26	6.83	6.83	CD
cord-297160-tqw9vx2b	118	27	,	,	,
cord-297160-tqw9vx2b	118	28	6.37	6.37	CD
cord-297160-tqw9vx2b	118	29	and	and	CC
cord-297160-tqw9vx2b	118	30	6.80	6.80	CD
cord-297160-tqw9vx2b	118	31	10	10	CD
cord-297160-tqw9vx2b	118	32	log	log	NN
cord-297160-tqw9vx2b	118	33	EID	EID	NNP
cord-297160-tqw9vx2b	118	34	50	50	CD
cord-297160-tqw9vx2b	118	35	respectively	respectively	RB
cord-297160-tqw9vx2b	118	36	,	,	,
cord-297160-tqw9vx2b	118	37	an	an	DT
cord-297160-tqw9vx2b	118	38	average	average	JJ
cord-297160-tqw9vx2b	118	39	titre	titre	NN
cord-297160-tqw9vx2b	118	40	per	per	IN
cord-297160-tqw9vx2b	118	41	vial	vial	NN
cord-297160-tqw9vx2b	118	42	was	be	VBD
cord-297160-tqw9vx2b	118	43	calculated	calculate	VBN
cord-297160-tqw9vx2b	118	44	,	,	,
cord-297160-tqw9vx2b	118	45	i.e.	i.e.	FW
cord-297160-tqw9vx2b	118	46	6.67	6.67	CD
cord-297160-tqw9vx2b	118	47	10	10	CD
cord-297160-tqw9vx2b	118	48	log	log	NN
cord-297160-tqw9vx2b	118	49	EID	EID	NNP
cord-297160-tqw9vx2b	118	50	50	50	CD
cord-297160-tqw9vx2b	118	51	or	or	CC
cord-297160-tqw9vx2b	118	52	10	10	CD
cord-297160-tqw9vx2b	118	53	6.67	6.67	CD
cord-297160-tqw9vx2b	118	54	EID	EID	NNP
cord-297160-tqw9vx2b	118	55	50	50	CD
cord-297160-tqw9vx2b	118	56	.	.	.
cord-297160-tqw9vx2b	119	1	Analysis	analysis	NN
cord-297160-tqw9vx2b	119	2	of	of	IN
cord-297160-tqw9vx2b	119	3	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	119	4	fluids	fluid	NNS
cord-297160-tqw9vx2b	119	5	demonstrated	demonstrate	VBN
cord-297160-tqw9vx2b	119	6	for	for	IN
cord-297160-tqw9vx2b	119	7	both	both	DT
cord-297160-tqw9vx2b	119	8	strains	strain	NNS
cord-297160-tqw9vx2b	119	9	there	there	EX
cord-297160-tqw9vx2b	119	10	were	be	VBD
cord-297160-tqw9vx2b	119	11	clear	clear	JJ
cord-297160-tqw9vx2b	119	12	endpoints	endpoint	NNS
cord-297160-tqw9vx2b	119	13	in	in	IN
cord-297160-tqw9vx2b	119	14	each	each	DT
cord-297160-tqw9vx2b	119	15	triplicate	triplicate	NN
cord-297160-tqw9vx2b	119	16	so	so	IN
cord-297160-tqw9vx2b	119	17	that	that	IN
cord-297160-tqw9vx2b	119	18	a	a	DT
cord-297160-tqw9vx2b	119	19	titre	titre	NN
cord-297160-tqw9vx2b	119	20	could	could	MD
cord-297160-tqw9vx2b	119	21	be	be	VB
cord-297160-tqw9vx2b	119	22	determined	determine	VBN
cord-297160-tqw9vx2b	119	23	in	in	IN
cord-297160-tqw9vx2b	119	24	a	a	DT
cord-297160-tqw9vx2b	119	25	correct	correct	JJ
cord-297160-tqw9vx2b	119	26	manner	manner	NN
cord-297160-tqw9vx2b	119	27	.	.	.
cord-297160-tqw9vx2b	120	1	The	the	DT
cord-297160-tqw9vx2b	120	2	average	average	JJ
cord-297160-tqw9vx2b	120	3	titres	titre	NNS
cord-297160-tqw9vx2b	120	4	determined	determine	VBN
cord-297160-tqw9vx2b	120	5	for	for	IN
cord-297160-tqw9vx2b	120	6	both	both	DT
cord-297160-tqw9vx2b	120	7	strains	strain	NNS
cord-297160-tqw9vx2b	120	8	were	be	VBD
cord-297160-tqw9vx2b	120	9	10	10	CD
cord-297160-tqw9vx2b	120	10	6.31	6.31	CD
cord-297160-tqw9vx2b	120	11	EID	EID	NNP
cord-297160-tqw9vx2b	120	12	50	50	CD
cord-297160-tqw9vx2b	120	13	per	per	IN
cord-297160-tqw9vx2b	120	14	vial	vial	NN
cord-297160-tqw9vx2b	120	15	for	for	IN
cord-297160-tqw9vx2b	120	16	IB	IB	NNP
cord-297160-tqw9vx2b	120	17	H120	H120	NNP
cord-297160-tqw9vx2b	120	18	and	and	CC
cord-297160-tqw9vx2b	120	19	10	10	CD
cord-297160-tqw9vx2b	120	20	6.59	6.59	CD
cord-297160-tqw9vx2b	120	21	EID	EID	NNP
cord-297160-tqw9vx2b	120	22	50	50	CD
cord-297160-tqw9vx2b	120	23	for	for	IN
cord-297160-tqw9vx2b	120	24	IB	IB	NNP
cord-297160-tqw9vx2b	120	25	D274	D274	NNP
cord-297160-tqw9vx2b	120	26	.	.	.
cord-297160-tqw9vx2b	121	1	If	if	IN
cord-297160-tqw9vx2b	121	2	the	the	DT
cord-297160-tqw9vx2b	121	3	sum	sum	NN
cord-297160-tqw9vx2b	121	4	of	of	IN
cord-297160-tqw9vx2b	121	5	these	these	DT
cord-297160-tqw9vx2b	121	6	2	2	CD
cord-297160-tqw9vx2b	121	7	values	value	NNS
cord-297160-tqw9vx2b	121	8	is	be	VBZ
cord-297160-tqw9vx2b	121	9	determined	determine	VBN
cord-297160-tqw9vx2b	121	10	the	the	DT
cord-297160-tqw9vx2b	121	11	titre	titre	NN
cord-297160-tqw9vx2b	121	12	is	be	VBZ
cord-297160-tqw9vx2b	121	13	10	10	CD
cord-297160-tqw9vx2b	121	14	6.77	6.77	CD
cord-297160-tqw9vx2b	121	15	EID	EID	NNP
cord-297160-tqw9vx2b	121	16	50	50	CD
cord-297160-tqw9vx2b	121	17	Table	table	NN
cord-297160-tqw9vx2b	121	18	2	2	CD
cord-297160-tqw9vx2b	121	19	Titration	titration	NN
cord-297160-tqw9vx2b	121	20	of	of	IN
cord-297160-tqw9vx2b	121	21	Poulvac	Poulvac	NNP
cord-297160-tqw9vx2b	121	22	IB	IB	NNP
cord-297160-tqw9vx2b	121	23	primer	primer	NN
cord-297160-tqw9vx2b	121	24	vaccine	vaccine	NN
cord-297160-tqw9vx2b	121	25	using	use	VBG
cord-297160-tqw9vx2b	121	26	strain	strain	NN
cord-297160-tqw9vx2b	121	27	specific	specific	JJ
cord-297160-tqw9vx2b	121	28	PCR	PCR	NNP
cord-297160-tqw9vx2b	121	29	for	for	IN
cord-297160-tqw9vx2b	121	30	detection	detection	NN
cord-297160-tqw9vx2b	121	31	of	of	IN
cord-297160-tqw9vx2b	121	32	IB	IB	NNP
cord-297160-tqw9vx2b	121	33	H120	H120	NNP
cord-297160-tqw9vx2b	121	34	or	or	CC
cord-297160-tqw9vx2b	121	35	IB	IB	NNP
cord-297160-tqw9vx2b	121	36	D174	D174	''
cord-297160-tqw9vx2b	121	37	.	.	.
cord-297160-tqw9vx2b	122	1	Results	result	NNS
cord-297160-tqw9vx2b	122	2	from	from	IN
cord-297160-tqw9vx2b	122	3	a	a	DT
cord-297160-tqw9vx2b	122	4	dilution	dilution	NN
cord-297160-tqw9vx2b	122	5	series	series	NN
cord-297160-tqw9vx2b	122	6	before	before	IN
cord-297160-tqw9vx2b	122	7	titration	titration	NN
cord-297160-tqw9vx2b	122	8	and	and	CC
cord-297160-tqw9vx2b	122	9	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	122	10	fluid	fluid	JJ
cord-297160-tqw9vx2b	122	11	pools	pool	NNS
cord-297160-tqw9vx2b	122	12	after	after	IN
cord-297160-tqw9vx2b	122	13	titration	titration	NN
cord-297160-tqw9vx2b	122	14	,	,	,
cord-297160-tqw9vx2b	122	15	and	and	CC
cord-297160-tqw9vx2b	122	16	results	result	NNS
cord-297160-tqw9vx2b	122	17	of	of	IN
cord-297160-tqw9vx2b	122	18	visual	visual	JJ
cord-297160-tqw9vx2b	122	19	examination	examination	NN
cord-297160-tqw9vx2b	122	20	after	after	IN
cord-297160-tqw9vx2b	122	21	titration	titration	NN
cord-297160-tqw9vx2b	122	22	are	be	VBP
cord-297160-tqw9vx2b	122	23	shown	show	VBN
cord-297160-tqw9vx2b	122	24	.	.	.
cord-297160-tqw9vx2b	122	25	 	 	_SP
cord-297160-tqw9vx2b	123	1	per	per	IN
cord-297160-tqw9vx2b	123	2	vial	vial	NN
cord-297160-tqw9vx2b	123	3	,	,	,
cord-297160-tqw9vx2b	123	4	which	which	WDT
cord-297160-tqw9vx2b	123	5	is	be	VBZ
cord-297160-tqw9vx2b	123	6	very	very	RB
cord-297160-tqw9vx2b	123	7	near	near	JJ
cord-297160-tqw9vx2b	123	8	to	to	IN
cord-297160-tqw9vx2b	123	9	the	the	DT
cord-297160-tqw9vx2b	123	10	value	value	NN
cord-297160-tqw9vx2b	123	11	of	of	IN
cord-297160-tqw9vx2b	123	12	10	10	CD
cord-297160-tqw9vx2b	123	13	6.67	6.67	CD
cord-297160-tqw9vx2b	123	14	EID	EID	NNP
cord-297160-tqw9vx2b	123	15	50	50	CD
cord-297160-tqw9vx2b	123	16	obtained	obtain	VBD
cord-297160-tqw9vx2b	123	17	after	after	IN
cord-297160-tqw9vx2b	123	18	visual	visual	JJ
cord-297160-tqw9vx2b	123	19	examination	examination	NN
cord-297160-tqw9vx2b	123	20	.	.	.
cord-297160-tqw9vx2b	124	1	Numerous	numerous	JJ
cord-297160-tqw9vx2b	124	2	publications	publication	NNS
cord-297160-tqw9vx2b	124	3	are	be	VBP
cord-297160-tqw9vx2b	124	4	available	available	JJ
cord-297160-tqw9vx2b	124	5	in	in	IN
cord-297160-tqw9vx2b	124	6	which	which	WDT
cord-297160-tqw9vx2b	124	7	viral	viral	JJ
cord-297160-tqw9vx2b	124	8	titrations	titration	NNS
cord-297160-tqw9vx2b	124	9	and	and	CC
cord-297160-tqw9vx2b	124	10	possible	possible	JJ
cord-297160-tqw9vx2b	124	11	issues	issue	NNS
cord-297160-tqw9vx2b	124	12	have	have	VBP
cord-297160-tqw9vx2b	124	13	been	be	VBN
cord-297160-tqw9vx2b	124	14	described	describe	VBN
cord-297160-tqw9vx2b	124	15	.	.	.
cord-297160-tqw9vx2b	125	1	For	for	IN
cord-297160-tqw9vx2b	125	2	several	several	JJ
cord-297160-tqw9vx2b	125	3	viruses	virus	NNS
cord-297160-tqw9vx2b	125	4	tissue	tissue	VBP
cord-297160-tqw9vx2b	125	5	culture	culture	NN
cord-297160-tqw9vx2b	125	6	methods	method	NNS
cord-297160-tqw9vx2b	125	7	can	can	MD
cord-297160-tqw9vx2b	125	8	be	be	VB
cord-297160-tqw9vx2b	125	9	used	use	VBN
cord-297160-tqw9vx2b	125	10	,	,	,
cord-297160-tqw9vx2b	125	11	in	in	IN
cord-297160-tqw9vx2b	125	12	which	which	WDT
cord-297160-tqw9vx2b	125	13	a	a	DT
cord-297160-tqw9vx2b	125	14	positive	positive	JJ
cord-297160-tqw9vx2b	125	15	response	response	NN
cord-297160-tqw9vx2b	125	16	can	can	MD
cord-297160-tqw9vx2b	125	17	be	be	VB
cord-297160-tqw9vx2b	125	18	observed	observe	VBN
cord-297160-tqw9vx2b	125	19	by	by	IN
cord-297160-tqw9vx2b	125	20	examination	examination	NN
cord-297160-tqw9vx2b	125	21	of	of	IN
cord-297160-tqw9vx2b	125	22	monolayers	monolayer	NNS
cord-297160-tqw9vx2b	125	23	of	of	IN
cord-297160-tqw9vx2b	125	24	cells	cell	NNS
cord-297160-tqw9vx2b	125	25	for	for	IN
cord-297160-tqw9vx2b	125	26	the	the	DT
cord-297160-tqw9vx2b	125	27	presence	presence	NN
cord-297160-tqw9vx2b	125	28	of	of	IN
cord-297160-tqw9vx2b	125	29	a	a	DT
cord-297160-tqw9vx2b	125	30	cytopathogenic	cytopathogenic	JJ
cord-297160-tqw9vx2b	125	31	effect	effect	NN
cord-297160-tqw9vx2b	125	32	(	(	-LRB-
cord-297160-tqw9vx2b	125	33	Heldt	Heldt	NNP
cord-297160-tqw9vx2b	125	34	et	et	NNP
cord-297160-tqw9vx2b	125	35	al	al	NNP
cord-297160-tqw9vx2b	125	36	.	.	NNP
cord-297160-tqw9vx2b	125	37	,	,	,
cord-297160-tqw9vx2b	125	38	2006	2006	CD
cord-297160-tqw9vx2b	125	39	)	)	-RRB-
cord-297160-tqw9vx2b	125	40	.	.	.
cord-297160-tqw9vx2b	126	1	If	if	IN
cord-297160-tqw9vx2b	126	2	the	the	DT
cord-297160-tqw9vx2b	126	3	virus	virus	NN
cord-297160-tqw9vx2b	126	4	does	do	VBZ
cord-297160-tqw9vx2b	126	5	not	not	RB
cord-297160-tqw9vx2b	126	6	form	form	VB
cord-297160-tqw9vx2b	126	7	a	a	DT
cord-297160-tqw9vx2b	126	8	cytopathogenic	cytopathogenic	JJ
cord-297160-tqw9vx2b	126	9	effect	effect	NN
cord-297160-tqw9vx2b	126	10	,	,	,
cord-297160-tqw9vx2b	126	11	the	the	DT
cord-297160-tqw9vx2b	126	12	presence	presence	NN
cord-297160-tqw9vx2b	126	13	of	of	IN
cord-297160-tqw9vx2b	126	14	live	live	JJ
cord-297160-tqw9vx2b	126	15	virus	virus	NN
cord-297160-tqw9vx2b	126	16	in	in	IN
cord-297160-tqw9vx2b	126	17	the	the	DT
cord-297160-tqw9vx2b	126	18	culture	culture	NN
cord-297160-tqw9vx2b	126	19	can	can	MD
cord-297160-tqw9vx2b	126	20	be	be	VB
cord-297160-tqw9vx2b	126	21	demonstrated	demonstrate	VBN
cord-297160-tqw9vx2b	126	22	by	by	IN
cord-297160-tqw9vx2b	126	23	methods	method	NNS
cord-297160-tqw9vx2b	126	24	using	use	VBG
cord-297160-tqw9vx2b	126	25	colour	colour	NN
cord-297160-tqw9vx2b	126	26	agents	agent	NNS
cord-297160-tqw9vx2b	126	27	(	(	-LRB-
cord-297160-tqw9vx2b	126	28	Heldt	Heldt	NNP
cord-297160-tqw9vx2b	126	29	et	et	NNP
cord-297160-tqw9vx2b	126	30	al	al	NNP
cord-297160-tqw9vx2b	126	31	.	.	NNP
cord-297160-tqw9vx2b	126	32	,	,	,
cord-297160-tqw9vx2b	126	33	2006	2006	CD
cord-297160-tqw9vx2b	126	34	)	)	-RRB-
cord-297160-tqw9vx2b	126	35	or	or	CC
cord-297160-tqw9vx2b	126	36	specific	specific	JJ
cord-297160-tqw9vx2b	126	37	antibodies	antibody	NNS
cord-297160-tqw9vx2b	126	38	(	(	-LRB-
cord-297160-tqw9vx2b	126	39	Zielinska	Zielinska	NNP
cord-297160-tqw9vx2b	126	40	et	et	FW
cord-297160-tqw9vx2b	126	41	al	al	NNP
cord-297160-tqw9vx2b	126	42	.	.	NNP
cord-297160-tqw9vx2b	126	43	,	,	,
cord-297160-tqw9vx2b	126	44	2005	2005	CD
cord-297160-tqw9vx2b	126	45	;	;	:
cord-297160-tqw9vx2b	126	46	Yap	Yap	NNP
cord-297160-tqw9vx2b	126	47	and	and	CC
cord-297160-tqw9vx2b	126	48	Lam	Lam	NNP
cord-297160-tqw9vx2b	126	49	,	,	,
cord-297160-tqw9vx2b	126	50	1994	1994	CD
cord-297160-tqw9vx2b	126	51	)	)	-RRB-
cord-297160-tqw9vx2b	126	52	.	.	.
cord-297160-tqw9vx2b	127	1	For	for	IN
cord-297160-tqw9vx2b	127	2	avian	avian	JJ
cord-297160-tqw9vx2b	127	3	viruses	virus	NNS
cord-297160-tqw9vx2b	127	4	suitable	suitable	JJ
cord-297160-tqw9vx2b	127	5	substrates	substrate	NNS
cord-297160-tqw9vx2b	127	6	are	be	VBP
cord-297160-tqw9vx2b	127	7	needed	need	VBN
cord-297160-tqw9vx2b	127	8	and	and	CC
cord-297160-tqw9vx2b	127	9	sometimes	sometimes	RB
cord-297160-tqw9vx2b	127	10	there	there	EX
cord-297160-tqw9vx2b	127	11	are	be	VBP
cord-297160-tqw9vx2b	127	12	different	different	JJ
cord-297160-tqw9vx2b	127	13	possibilities	possibility	NNS
cord-297160-tqw9vx2b	127	14	(	(	-LRB-
cord-297160-tqw9vx2b	127	15	Nicholas	Nicholas	NNP
cord-297160-tqw9vx2b	127	16	et	et	NNP
cord-297160-tqw9vx2b	127	17	al	al	NNP
cord-297160-tqw9vx2b	127	18	.	.	NNP
cord-297160-tqw9vx2b	127	19	,	,	,
cord-297160-tqw9vx2b	127	20	1986	1986	CD
cord-297160-tqw9vx2b	127	21	)	)	-RRB-
cord-297160-tqw9vx2b	127	22	.	.	.
cord-297160-tqw9vx2b	128	1	For	for	IN
cord-297160-tqw9vx2b	128	2	titrations	titration	NNS
cord-297160-tqw9vx2b	128	3	in	in	IN
cord-297160-tqw9vx2b	128	4	embryonated	embryonate	VBN
cord-297160-tqw9vx2b	128	5	eggs	egg	NNS
cord-297160-tqw9vx2b	128	6	the	the	DT
cord-297160-tqw9vx2b	128	7	presence	presence	NN
cord-297160-tqw9vx2b	128	8	of	of	IN
cord-297160-tqw9vx2b	128	9	the	the	DT
cord-297160-tqw9vx2b	128	10	virus	virus	NN
cord-297160-tqw9vx2b	128	11	in	in	IN
cord-297160-tqw9vx2b	128	12	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	128	13	fluid	fluid	NN
cord-297160-tqw9vx2b	128	14	can	can	MD
cord-297160-tqw9vx2b	128	15	be	be	VB
cord-297160-tqw9vx2b	128	16	tested	test	VBN
cord-297160-tqw9vx2b	128	17	using	use	VBG
cord-297160-tqw9vx2b	128	18	a	a	DT
cord-297160-tqw9vx2b	128	19	specific	specific	JJ
cord-297160-tqw9vx2b	128	20	property	property	NN
cord-297160-tqw9vx2b	128	21	of	of	IN
cord-297160-tqw9vx2b	128	22	the	the	DT
cord-297160-tqw9vx2b	128	23	virus	virus	NN
cord-297160-tqw9vx2b	128	24	,	,	,
cord-297160-tqw9vx2b	128	25	for	for	IN
cord-297160-tqw9vx2b	128	26	example	example	NN
cord-297160-tqw9vx2b	128	27	its	-PRON-	PRP$
cord-297160-tqw9vx2b	128	28	ability	ability	NN
cord-297160-tqw9vx2b	128	29	to	to	TO
cord-297160-tqw9vx2b	128	30	cause	cause	VB
cord-297160-tqw9vx2b	128	31	haemagglutination	haemagglutination	NN
cord-297160-tqw9vx2b	128	32	(	(	-LRB-
cord-297160-tqw9vx2b	128	33	Klimov	Klimov	NNP
cord-297160-tqw9vx2b	128	34	et	et	NNP
cord-297160-tqw9vx2b	128	35	al	al	NNP
cord-297160-tqw9vx2b	128	36	.	.	NNP
cord-297160-tqw9vx2b	128	37	,	,	,
cord-297160-tqw9vx2b	128	38	2012	2012	CD
cord-297160-tqw9vx2b	128	39	)	)	-RRB-
cord-297160-tqw9vx2b	128	40	.	.	.
cord-297160-tqw9vx2b	129	1	The	the	DT
cord-297160-tqw9vx2b	129	2	presence	presence	NN
cord-297160-tqw9vx2b	129	3	or	or	CC
cord-297160-tqw9vx2b	129	4	absence	absence	NN
cord-297160-tqw9vx2b	129	5	of	of	IN
cord-297160-tqw9vx2b	129	6	lesions	lesion	NNS
cord-297160-tqw9vx2b	129	7	on	on	IN
cord-297160-tqw9vx2b	129	8	the	the	DT
cord-297160-tqw9vx2b	129	9	embryos	embryo	NNS
cord-297160-tqw9vx2b	129	10	also	also	RB
cord-297160-tqw9vx2b	129	11	can	can	MD
cord-297160-tqw9vx2b	129	12	be	be	VB
cord-297160-tqw9vx2b	129	13	used	use	VBN
cord-297160-tqw9vx2b	129	14	to	to	TO
cord-297160-tqw9vx2b	129	15	determine	determine	VB
cord-297160-tqw9vx2b	129	16	the	the	DT
cord-297160-tqw9vx2b	129	17	presence	presence	NN
cord-297160-tqw9vx2b	129	18	of	of	IN
cord-297160-tqw9vx2b	129	19	live	live	JJ
cord-297160-tqw9vx2b	129	20	virus	virus	NN
cord-297160-tqw9vx2b	129	21	,	,	,
cord-297160-tqw9vx2b	129	22	such	such	JJ
cord-297160-tqw9vx2b	129	23	as	as	IN
cord-297160-tqw9vx2b	129	24	has	have	VBZ
cord-297160-tqw9vx2b	129	25	been	be	VBN
cord-297160-tqw9vx2b	129	26	demonstrated	demonstrate	VBN
cord-297160-tqw9vx2b	129	27	for	for	IN
cord-297160-tqw9vx2b	129	28	IB	IB	NNP
cord-297160-tqw9vx2b	129	29	viruses	virus	NNS
cord-297160-tqw9vx2b	129	30	(	(	-LRB-
cord-297160-tqw9vx2b	129	31	De	De	NNP
cord-297160-tqw9vx2b	129	32	Wit	Wit	NNP
cord-297160-tqw9vx2b	129	33	,	,	,
cord-297160-tqw9vx2b	129	34	2000	2000	CD
cord-297160-tqw9vx2b	129	35	)	)	-RRB-
cord-297160-tqw9vx2b	129	36	.	.	.
cord-297160-tqw9vx2b	130	1	The	the	DT
cord-297160-tqw9vx2b	130	2	use	use	NN
cord-297160-tqw9vx2b	130	3	of	of	IN
cord-297160-tqw9vx2b	130	4	PCR	PCR	NNP
cord-297160-tqw9vx2b	130	5	for	for	IN
cord-297160-tqw9vx2b	130	6	screening	screening	NN
cord-297160-tqw9vx2b	130	7	samples	sample	NNS
cord-297160-tqw9vx2b	130	8	from	from	IN
cord-297160-tqw9vx2b	130	9	titrations	titration	NNS
cord-297160-tqw9vx2b	130	10	for	for	IN
cord-297160-tqw9vx2b	130	11	the	the	DT
cord-297160-tqw9vx2b	130	12	presence	presence	NN
cord-297160-tqw9vx2b	130	13	of	of	IN
cord-297160-tqw9vx2b	130	14	virus	virus	NN
cord-297160-tqw9vx2b	130	15	has	have	VBZ
cord-297160-tqw9vx2b	130	16	not	not	RB
cord-297160-tqw9vx2b	130	17	been	be	VBN
cord-297160-tqw9vx2b	130	18	described	describe	VBN
cord-297160-tqw9vx2b	130	19	previously	previously	RB
cord-297160-tqw9vx2b	130	20	.	.	.
cord-297160-tqw9vx2b	131	1	Initially	initially	RB
cord-297160-tqw9vx2b	131	2	PCR	PCR	NNP
cord-297160-tqw9vx2b	131	3	was	be	VBD
cord-297160-tqw9vx2b	131	4	used	use	VBN
cord-297160-tqw9vx2b	131	5	mainly	mainly	RB
cord-297160-tqw9vx2b	131	6	for	for	IN
cord-297160-tqw9vx2b	131	7	testing	testing	NN
cord-297160-tqw9vx2b	131	8	for	for	IN
cord-297160-tqw9vx2b	131	9	the	the	DT
cord-297160-tqw9vx2b	131	10	presence	presence	NN
cord-297160-tqw9vx2b	131	11	of	of	IN
cord-297160-tqw9vx2b	131	12	viruses	virus	NNS
cord-297160-tqw9vx2b	131	13	or	or	CC
cord-297160-tqw9vx2b	131	14	other	other	JJ
cord-297160-tqw9vx2b	131	15	infectious	infectious	JJ
cord-297160-tqw9vx2b	131	16	agents	agent	NNS
cord-297160-tqw9vx2b	131	17	in	in	IN
cord-297160-tqw9vx2b	131	18	samples	sample	NNS
cord-297160-tqw9vx2b	132	1	(	(	-LRB-
cord-297160-tqw9vx2b	132	2	Tafuro	Tafuro	NNP
cord-297160-tqw9vx2b	132	3	et	et	NNP
cord-297160-tqw9vx2b	132	4	al	al	NNP
cord-297160-tqw9vx2b	132	5	.	.	NNP
cord-297160-tqw9vx2b	132	6	,	,	,
cord-297160-tqw9vx2b	132	7	1996	1996	CD
cord-297160-tqw9vx2b	132	8	)	)	-RRB-
cord-297160-tqw9vx2b	132	9	.	.	.
cord-297160-tqw9vx2b	133	1	Currently	currently	RB
cord-297160-tqw9vx2b	133	2	,	,	,
cord-297160-tqw9vx2b	133	3	with	with	IN
cord-297160-tqw9vx2b	133	4	the	the	DT
cord-297160-tqw9vx2b	133	5	introduction	introduction	NN
cord-297160-tqw9vx2b	133	6	of	of	IN
cord-297160-tqw9vx2b	133	7	real	real	JJ
cord-297160-tqw9vx2b	133	8	time	time	NN
cord-297160-tqw9vx2b	133	9	PCR	PCR	NNP
cord-297160-tqw9vx2b	133	10	it	-PRON-	PRP
cord-297160-tqw9vx2b	133	11	is	be	VBZ
cord-297160-tqw9vx2b	133	12	possible	possible	JJ
cord-297160-tqw9vx2b	133	13	to	to	TO
cord-297160-tqw9vx2b	133	14	determine	determine	VB
cord-297160-tqw9vx2b	133	15	quantities	quantity	NNS
cord-297160-tqw9vx2b	133	16	of	of	IN
cord-297160-tqw9vx2b	133	17	virus	virus	NN
cord-297160-tqw9vx2b	133	18	particles	particle	NNS
cord-297160-tqw9vx2b	133	19	in	in	IN
cord-297160-tqw9vx2b	133	20	samples	sample	NNS
cord-297160-tqw9vx2b	133	21	(	(	-LRB-
cord-297160-tqw9vx2b	133	22	Hiroshi	Hiroshi	NNP
cord-297160-tqw9vx2b	133	23	Kimura	Kimura	NNP
cord-297160-tqw9vx2b	133	24	et	et	FW
cord-297160-tqw9vx2b	133	25	al	al	NNP
cord-297160-tqw9vx2b	133	26	.	.	NNP
cord-297160-tqw9vx2b	133	27	,	,	,
cord-297160-tqw9vx2b	133	28	1999	1999	CD
cord-297160-tqw9vx2b	133	29	;	;	:
cord-297160-tqw9vx2b	133	30	Isabel	Isabel	NNP
cord-297160-tqw9vx2b	133	31	Costafreda	Costafreda	NNP
cord-297160-tqw9vx2b	133	32	et	et	NNP
cord-297160-tqw9vx2b	133	33	al	al	NNP
cord-297160-tqw9vx2b	133	34	.	.	.
cord-297160-tqw9vx2b	134	1	,	,	,
cord-297160-tqw9vx2b	134	2	2006	2006	CD
cord-297160-tqw9vx2b	134	3	;	;	:
cord-297160-tqw9vx2b	134	4	Spackman	Spackman	NNP
cord-297160-tqw9vx2b	134	5	and	and	CC
cord-297160-tqw9vx2b	134	6	Suarez	Suarez	NNP
cord-297160-tqw9vx2b	134	7	,	,	,
cord-297160-tqw9vx2b	134	8	2008	2008	CD
cord-297160-tqw9vx2b	134	9	)	)	-RRB-
cord-297160-tqw9vx2b	134	10	.	.	.
cord-297160-tqw9vx2b	135	1	Because	because	IN
cord-297160-tqw9vx2b	135	2	of	of	IN
cord-297160-tqw9vx2b	135	3	its	-PRON-	PRP$
cord-297160-tqw9vx2b	135	4	specificity	specificity	NN
cord-297160-tqw9vx2b	135	5	it	-PRON-	PRP
cord-297160-tqw9vx2b	135	6	is	be	VBZ
cord-297160-tqw9vx2b	135	7	possible	possible	JJ
cord-297160-tqw9vx2b	135	8	also	also	RB
cord-297160-tqw9vx2b	135	9	to	to	TO
cord-297160-tqw9vx2b	135	10	detect	detect	VB
cord-297160-tqw9vx2b	135	11	more	more	JJR
cord-297160-tqw9vx2b	135	12	than	than	IN
cord-297160-tqw9vx2b	135	13	one	one	CD
cord-297160-tqw9vx2b	135	14	virus	virus	NN
cord-297160-tqw9vx2b	135	15	strain	strain	NN
cord-297160-tqw9vx2b	135	16	in	in	IN
cord-297160-tqw9vx2b	135	17	one	one	CD
cord-297160-tqw9vx2b	135	18	test	test	NN
cord-297160-tqw9vx2b	135	19	using	use	VBG
cord-297160-tqw9vx2b	135	20	PCR	PCR	NNP
cord-297160-tqw9vx2b	135	21	(	(	-LRB-
cord-297160-tqw9vx2b	135	22	Van	Van	NNP
cord-297160-tqw9vx2b	135	23	Elden	Elden	NNP
cord-297160-tqw9vx2b	135	24	et	et	NNP
cord-297160-tqw9vx2b	135	25	al	al	NNP
cord-297160-tqw9vx2b	135	26	.	.	NNP
cord-297160-tqw9vx2b	135	27	,	,	,
cord-297160-tqw9vx2b	135	28	2001	2001	CD
cord-297160-tqw9vx2b	135	29	)	)	-RRB-
cord-297160-tqw9vx2b	135	30	.	.	.
cord-297160-tqw9vx2b	136	1	We	-PRON-	PRP
cord-297160-tqw9vx2b	136	2	used	use	VBD
cord-297160-tqw9vx2b	136	3	PCR	PCR	NNP
cord-297160-tqw9vx2b	136	4	for	for	IN
cord-297160-tqw9vx2b	136	5	detection	detection	NN
cord-297160-tqw9vx2b	136	6	of	of	IN
cord-297160-tqw9vx2b	136	7	two	two	CD
cord-297160-tqw9vx2b	136	8	different	different	JJ
cord-297160-tqw9vx2b	136	9	IB	IB	NNP
cord-297160-tqw9vx2b	136	10	virus	virus	NN
cord-297160-tqw9vx2b	136	11	strains	strain	NNS
cord-297160-tqw9vx2b	136	12	in	in	IN
cord-297160-tqw9vx2b	136	13	the	the	DT
cord-297160-tqw9vx2b	136	14	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	136	15	fluids	fluid	VBZ
cord-297160-tqw9vx2b	136	16	from	from	IN
cord-297160-tqw9vx2b	136	17	all	all	PDT
cord-297160-tqw9vx2b	136	18	the	the	DT
cord-297160-tqw9vx2b	136	19	individual	individual	JJ
cord-297160-tqw9vx2b	136	20	eggs	egg	NNS
cord-297160-tqw9vx2b	136	21	in	in	IN
cord-297160-tqw9vx2b	136	22	a	a	DT
cord-297160-tqw9vx2b	136	23	titration	titration	NN
cord-297160-tqw9vx2b	136	24	of	of	IN
cord-297160-tqw9vx2b	136	25	a	a	DT
cord-297160-tqw9vx2b	136	26	live	live	JJ
cord-297160-tqw9vx2b	136	27	IB	IB	NNP
cord-297160-tqw9vx2b	136	28	combination	combination	NN
cord-297160-tqw9vx2b	136	29	vaccine	vaccine	NN
cord-297160-tqw9vx2b	136	30	.	.	.
cord-297160-tqw9vx2b	137	1	Based	base	VBN
cord-297160-tqw9vx2b	137	2	on	on	IN
cord-297160-tqw9vx2b	137	3	the	the	DT
cord-297160-tqw9vx2b	137	4	results	result	NNS
cord-297160-tqw9vx2b	137	5	of	of	IN
cord-297160-tqw9vx2b	137	6	the	the	DT
cord-297160-tqw9vx2b	137	7	PCR	PCR	NNP
cord-297160-tqw9vx2b	137	8	we	-PRON-	PRP
cord-297160-tqw9vx2b	137	9	determined	determine	VBD
cord-297160-tqw9vx2b	137	10	whether	whether	IN
cord-297160-tqw9vx2b	137	11	an	an	DT
cord-297160-tqw9vx2b	137	12	allantoic	allantoic	NN
cord-297160-tqw9vx2b	137	13	fluid	fluid	NN
cord-297160-tqw9vx2b	137	14	was	be	VBD
cord-297160-tqw9vx2b	137	15	positive	positive	JJ
cord-297160-tqw9vx2b	137	16	for	for	IN
cord-297160-tqw9vx2b	137	17	one	one	CD
cord-297160-tqw9vx2b	137	18	or	or	CC
cord-297160-tqw9vx2b	137	19	both	both	DT
cord-297160-tqw9vx2b	137	20	of	of	IN
cord-297160-tqw9vx2b	137	21	the	the	DT
cord-297160-tqw9vx2b	137	22	viruses	virus	NNS
cord-297160-tqw9vx2b	137	23	or	or	CC
cord-297160-tqw9vx2b	137	24	not	not	RB
cord-297160-tqw9vx2b	137	25	,	,	,
cord-297160-tqw9vx2b	137	26	and	and	CC
cord-297160-tqw9vx2b	137	27	as	as	RB
cord-297160-tqw9vx2b	137	28	such	such	JJ
cord-297160-tqw9vx2b	137	29	we	-PRON-	PRP
cord-297160-tqw9vx2b	137	30	were	be	VBD
cord-297160-tqw9vx2b	137	31	able	able	JJ
cord-297160-tqw9vx2b	137	32	to	to	TO
cord-297160-tqw9vx2b	137	33	define	define	VB
cord-297160-tqw9vx2b	137	34	a	a	DT
cord-297160-tqw9vx2b	137	35	separate	separate	JJ
cord-297160-tqw9vx2b	137	36	endpoint	endpoint	NN
cord-297160-tqw9vx2b	137	37	in	in	IN
cord-297160-tqw9vx2b	137	38	the	the	DT
cord-297160-tqw9vx2b	137	39	live	live	JJ
cord-297160-tqw9vx2b	137	40	virus	virus	NN
cord-297160-tqw9vx2b	137	41	titration	titration	NN
cord-297160-tqw9vx2b	137	42	for	for	IN
cord-297160-tqw9vx2b	137	43	each	each	DT
cord-297160-tqw9vx2b	137	44	of	of	IN
cord-297160-tqw9vx2b	137	45	the	the	DT
cord-297160-tqw9vx2b	137	46	two	two	CD
cord-297160-tqw9vx2b	137	47	strains	strain	NNS
cord-297160-tqw9vx2b	137	48	.	.	.
cord-297160-tqw9vx2b	138	1	It	-PRON-	PRP
cord-297160-tqw9vx2b	138	2	has	have	VBZ
cord-297160-tqw9vx2b	138	3	to	to	TO
cord-297160-tqw9vx2b	138	4	be	be	VB
cord-297160-tqw9vx2b	138	5	clear	clear	JJ
cord-297160-tqw9vx2b	138	6	that	that	IN
cord-297160-tqw9vx2b	138	7	this	this	DT
cord-297160-tqw9vx2b	138	8	is	be	VBZ
cord-297160-tqw9vx2b	138	9	not	not	RB
cord-297160-tqw9vx2b	138	10	the	the	DT
cord-297160-tqw9vx2b	138	11	same	same	JJ
cord-297160-tqw9vx2b	138	12	as	as	IN
cord-297160-tqw9vx2b	138	13	a	a	DT
cord-297160-tqw9vx2b	138	14	quantitative	quantitative	JJ
cord-297160-tqw9vx2b	138	15	PCR	pcr	NN
cord-297160-tqw9vx2b	138	16	.	.	.
cord-297160-tqw9vx2b	139	1	In	in	IN
cord-297160-tqw9vx2b	139	2	a	a	DT
cord-297160-tqw9vx2b	139	3	quantitative	quantitative	JJ
cord-297160-tqw9vx2b	139	4	PCR	pcr	NN
cord-297160-tqw9vx2b	139	5	the	the	DT
cord-297160-tqw9vx2b	139	6	number	number	NN
cord-297160-tqw9vx2b	139	7	of	of	IN
cord-297160-tqw9vx2b	139	8	genomic	genomic	JJ
cord-297160-tqw9vx2b	139	9	fragments	fragment	NNS
cord-297160-tqw9vx2b	139	10	is	be	VBZ
cord-297160-tqw9vx2b	139	11	determined	determine	VBN
cord-297160-tqw9vx2b	139	12	based	base	VBN
cord-297160-tqw9vx2b	139	13	on	on	IN
cord-297160-tqw9vx2b	139	14	the	the	DT
cord-297160-tqw9vx2b	139	15	number	number	NN
cord-297160-tqw9vx2b	139	16	of	of	IN
cord-297160-tqw9vx2b	139	17	cycles	cycle	NNS
cord-297160-tqw9vx2b	139	18	in	in	IN
cord-297160-tqw9vx2b	139	19	the	the	DT
cord-297160-tqw9vx2b	139	20	real	real	JJ
cord-297160-tqw9vx2b	139	21	time	time	NN
cord-297160-tqw9vx2b	139	22	PCR	PCR	NNP
cord-297160-tqw9vx2b	139	23	required	require	VBN
cord-297160-tqw9vx2b	139	24	for	for	IN
cord-297160-tqw9vx2b	139	25	a	a	DT
cord-297160-tqw9vx2b	139	26	positive	positive	JJ
cord-297160-tqw9vx2b	139	27	response	response	NN
cord-297160-tqw9vx2b	139	28	.	.	.
cord-297160-tqw9vx2b	140	1	In	in	IN
cord-297160-tqw9vx2b	140	2	such	such	PDT
cord-297160-tqw9vx2b	140	3	a	a	DT
cord-297160-tqw9vx2b	140	4	PCR	PCR	NNP
cord-297160-tqw9vx2b	140	5	genomic	genomic	NN
cord-297160-tqw9vx2b	140	6	fragments	fragment	NNS
cord-297160-tqw9vx2b	140	7	coming	come	VBG
cord-297160-tqw9vx2b	140	8	from	from	IN
cord-297160-tqw9vx2b	140	9	live	live	RB
cord-297160-tqw9vx2b	140	10	as	as	RB
cord-297160-tqw9vx2b	140	11	well	well	RB
cord-297160-tqw9vx2b	140	12	as	as	IN
cord-297160-tqw9vx2b	140	13	'	'	``
cord-297160-tqw9vx2b	140	14	dead	dead	JJ
cord-297160-tqw9vx2b	140	15	'	'	''
cord-297160-tqw9vx2b	140	16	virus	virus	NN
cord-297160-tqw9vx2b	140	17	particles	particle	NNS
cord-297160-tqw9vx2b	140	18	are	be	VBP
cord-297160-tqw9vx2b	140	19	determined	determine	VBN
cord-297160-tqw9vx2b	140	20	.	.	.
cord-297160-tqw9vx2b	141	1	In	in	IN
cord-297160-tqw9vx2b	141	2	the	the	DT
cord-297160-tqw9vx2b	141	3	test	test	NN
cord-297160-tqw9vx2b	141	4	described	describe	VBN
cord-297160-tqw9vx2b	141	5	in	in	IN
cord-297160-tqw9vx2b	141	6	this	this	DT
cord-297160-tqw9vx2b	141	7	paper	paper	NN
cord-297160-tqw9vx2b	141	8	we	-PRON-	PRP
cord-297160-tqw9vx2b	141	9	determined	determine	VBD
cord-297160-tqw9vx2b	141	10	whether	whether	IN
cord-297160-tqw9vx2b	141	11	an	an	DT
cord-297160-tqw9vx2b	141	12	egg	egg	NN
cord-297160-tqw9vx2b	141	13	was	be	VBD
cord-297160-tqw9vx2b	141	14	positive	positive	JJ
cord-297160-tqw9vx2b	141	15	for	for	IN
cord-297160-tqw9vx2b	141	16	the	the	DT
cord-297160-tqw9vx2b	141	17	presence	presence	NN
cord-297160-tqw9vx2b	141	18	of	of	IN
cord-297160-tqw9vx2b	141	19	virus	virus	NN
cord-297160-tqw9vx2b	141	20	coming	come	VBG
cord-297160-tqw9vx2b	141	21	from	from	IN
cord-297160-tqw9vx2b	141	22	a	a	DT
cord-297160-tqw9vx2b	141	23	productive	productive	JJ
cord-297160-tqw9vx2b	141	24	infection	infection	NN
cord-297160-tqw9vx2b	141	25	using	use	VBG
cord-297160-tqw9vx2b	141	26	PCR	PCR	NNP
cord-297160-tqw9vx2b	141	27	with	with	IN
cord-297160-tqw9vx2b	141	28	a	a	DT
cord-297160-tqw9vx2b	141	29	fixed	fix	VBN
cord-297160-tqw9vx2b	141	30	number	number	NN
cord-297160-tqw9vx2b	141	31	of	of	IN
cord-297160-tqw9vx2b	141	32	cycles	cycle	NNS
cord-297160-tqw9vx2b	141	33	.	.	.
cord-297160-tqw9vx2b	142	1	Only	only	RB
cord-297160-tqw9vx2b	142	2	a	a	DT
cord-297160-tqw9vx2b	142	3	live	live	JJ
cord-297160-tqw9vx2b	142	4	virus	virus	NN
cord-297160-tqw9vx2b	142	5	particle	particle	NN
cord-297160-tqw9vx2b	142	6	can	can	MD
cord-297160-tqw9vx2b	142	7	cause	cause	VB
cord-297160-tqw9vx2b	142	8	a	a	DT
cord-297160-tqw9vx2b	142	9	productive	productive	JJ
cord-297160-tqw9vx2b	142	10	infection	infection	NN
cord-297160-tqw9vx2b	142	11	.	.	.
cord-297160-tqw9vx2b	143	1	If	if	IN
cord-297160-tqw9vx2b	143	2	the	the	DT
cord-297160-tqw9vx2b	143	3	egg	egg	NN
cord-297160-tqw9vx2b	143	4	would	would	MD
cord-297160-tqw9vx2b	143	5	have	have	VB
cord-297160-tqw9vx2b	143	6	been	be	VBN
cord-297160-tqw9vx2b	143	7	inoculated	inoculate	VBN
cord-297160-tqw9vx2b	143	8	with	with	IN
cord-297160-tqw9vx2b	143	9	a	a	DT
cord-297160-tqw9vx2b	143	10	dead	dead	JJ
cord-297160-tqw9vx2b	143	11	virus	virus	NN
cord-297160-tqw9vx2b	143	12	particle	particle	NN
cord-297160-tqw9vx2b	143	13	,	,	,
cord-297160-tqw9vx2b	143	14	it	-PRON-	PRP
cord-297160-tqw9vx2b	143	15	would	would	MD
cord-297160-tqw9vx2b	143	16	not	not	RB
cord-297160-tqw9vx2b	143	17	have	have	VB
cord-297160-tqw9vx2b	143	18	given	give	VBN
cord-297160-tqw9vx2b	143	19	a	a	DT
cord-297160-tqw9vx2b	143	20	positive	positive	JJ
cord-297160-tqw9vx2b	143	21	response	response	NN
cord-297160-tqw9vx2b	143	22	in	in	IN
cord-297160-tqw9vx2b	143	23	the	the	DT
cord-297160-tqw9vx2b	143	24	PCR	PCR	NNP
cord-297160-tqw9vx2b	143	25	.	.	.
cord-297160-tqw9vx2b	144	1	The	the	DT
cord-297160-tqw9vx2b	144	2	titration	titration	NN
cord-297160-tqw9vx2b	144	3	method	method	NN
cord-297160-tqw9vx2b	144	4	using	use	VBG
cord-297160-tqw9vx2b	144	5	PCR	PCR	NNP
cord-297160-tqw9vx2b	144	6	for	for	IN
cord-297160-tqw9vx2b	144	7	testing	test	VBG
cord-297160-tqw9vx2b	144	8	the	the	DT
cord-297160-tqw9vx2b	144	9	allantoic	allantoic	NNP
cord-297160-tqw9vx2b	144	10	fluids	fluid	NNS
cord-297160-tqw9vx2b	144	11	is	be	VBZ
cord-297160-tqw9vx2b	144	12	highly	highly	RB
cord-297160-tqw9vx2b	144	13	suitable	suitable	JJ
cord-297160-tqw9vx2b	144	14	to	to	TO
cord-297160-tqw9vx2b	144	15	determine	determine	VB
cord-297160-tqw9vx2b	144	16	titres	titre	NNS
cord-297160-tqw9vx2b	144	17	of	of	IN
cord-297160-tqw9vx2b	144	18	different	different	JJ
cord-297160-tqw9vx2b	144	19	vaccine	vaccine	NN
cord-297160-tqw9vx2b	144	20	strains	strain	NNS
cord-297160-tqw9vx2b	144	21	in	in	IN
cord-297160-tqw9vx2b	144	22	one	one	CD
cord-297160-tqw9vx2b	144	23	presentation	presentation	NN
cord-297160-tqw9vx2b	144	24	.	.	.
cord-297160-tqw9vx2b	145	1	The	the	DT
cord-297160-tqw9vx2b	145	2	PCR	PCR	NNP
cord-297160-tqw9vx2b	145	3	could	could	MD
cord-297160-tqw9vx2b	145	4	be	be	VB
cord-297160-tqw9vx2b	145	5	used	use	VBN
cord-297160-tqw9vx2b	145	6	also	also	RB
cord-297160-tqw9vx2b	145	7	for	for	IN
cord-297160-tqw9vx2b	145	8	end	end	NN
cord-297160-tqw9vx2b	145	9	-	-	HYPH
cord-297160-tqw9vx2b	145	10	point	point	NN
cord-297160-tqw9vx2b	145	11	determinations	determination	NNS
cord-297160-tqw9vx2b	145	12	of	of	IN
cord-297160-tqw9vx2b	145	13	other	other	JJ
cord-297160-tqw9vx2b	145	14	IB	IB	NNP
cord-297160-tqw9vx2b	145	15	strains	strain	NNS
cord-297160-tqw9vx2b	145	16	,	,	,
cord-297160-tqw9vx2b	145	17	for	for	IN
cord-297160-tqw9vx2b	145	18	example	example	NN
cord-297160-tqw9vx2b	145	19	IB	IB	NNP
cord-297160-tqw9vx2b	145	20	strains	strain	NNS
cord-297160-tqw9vx2b	145	21	which	which	WDT
cord-297160-tqw9vx2b	145	22	do	do	VBP
cord-297160-tqw9vx2b	145	23	not	not	RB
cord-297160-tqw9vx2b	145	24	have	have	VB
cord-297160-tqw9vx2b	145	25	a	a	DT
cord-297160-tqw9vx2b	145	26	very	very	RB
cord-297160-tqw9vx2b	145	27	clear	clear	JJ
cord-297160-tqw9vx2b	145	28	effect	effect	NN
cord-297160-tqw9vx2b	145	29	on	on	IN
cord-297160-tqw9vx2b	145	30	embryos	embryo	NNS
cord-297160-tqw9vx2b	145	31	making	make	VBG
cord-297160-tqw9vx2b	145	32	it	-PRON-	PRP
cord-297160-tqw9vx2b	145	33	difficult	difficult	JJ
cord-297160-tqw9vx2b	145	34	to	to	TO
cord-297160-tqw9vx2b	145	35	read	read	VB
cord-297160-tqw9vx2b	145	36	egg	egg	NN
cord-297160-tqw9vx2b	145	37	titrations	titration	NNS
cord-297160-tqw9vx2b	145	38	visually	visually	RB
cord-297160-tqw9vx2b	145	39	.	.	.
cord-297160-tqw9vx2b	146	1	Furthermore	furthermore	RB
cord-297160-tqw9vx2b	146	2	,	,	,
cord-297160-tqw9vx2b	146	3	the	the	DT
cord-297160-tqw9vx2b	146	4	use	use	NN
cord-297160-tqw9vx2b	146	5	of	of	IN
cord-297160-tqw9vx2b	146	6	PCR	PCR	NNP
cord-297160-tqw9vx2b	146	7	for	for	IN
cord-297160-tqw9vx2b	146	8	end	end	NN
cord-297160-tqw9vx2b	146	9	-	-	HYPH
cord-297160-tqw9vx2b	146	10	point	point	NN
cord-297160-tqw9vx2b	146	11	determination	determination	NN
cord-297160-tqw9vx2b	146	12	makes	make	VBZ
cord-297160-tqw9vx2b	146	13	it	-PRON-	PRP
cord-297160-tqw9vx2b	146	14	possible	possible	JJ
cord-297160-tqw9vx2b	146	15	also	also	RB
cord-297160-tqw9vx2b	146	16	to	to	TO
cord-297160-tqw9vx2b	146	17	perform	perform	VB
cord-297160-tqw9vx2b	146	18	titrations	titration	NNS
cord-297160-tqw9vx2b	146	19	on	on	IN
cord-297160-tqw9vx2b	146	20	a	a	DT
cord-297160-tqw9vx2b	146	21	large	large	JJ
cord-297160-tqw9vx2b	146	22	scale	scale	NN
cord-297160-tqw9vx2b	146	23	using	use	VBG
cord-297160-tqw9vx2b	146	24	specialized	specialized	JJ
cord-297160-tqw9vx2b	146	25	equipment	equipment	NN
cord-297160-tqw9vx2b	146	26	.	.	.
cord-297160-tqw9vx2b	147	1	It	-PRON-	PRP
cord-297160-tqw9vx2b	147	2	was	be	VBD
cord-297160-tqw9vx2b	147	3	demonstrated	demonstrate	VBN
cord-297160-tqw9vx2b	147	4	that	that	IN
cord-297160-tqw9vx2b	147	5	PCR	PCR	NNP
cord-297160-tqw9vx2b	147	6	is	be	VBZ
cord-297160-tqw9vx2b	147	7	a	a	DT
cord-297160-tqw9vx2b	147	8	suitable	suitable	JJ
cord-297160-tqw9vx2b	147	9	means	mean	NNS
cord-297160-tqw9vx2b	147	10	to	to	TO
cord-297160-tqw9vx2b	147	11	determine	determine	VB
cord-297160-tqw9vx2b	147	12	end	end	NN
cord-297160-tqw9vx2b	147	13	-	-	HYPH
cord-297160-tqw9vx2b	147	14	points	point	NNS
cord-297160-tqw9vx2b	147	15	in	in	IN
cord-297160-tqw9vx2b	147	16	titrations	titration	NNS
cord-297160-tqw9vx2b	147	17	of	of	IN
cord-297160-tqw9vx2b	147	18	live	live	JJ
cord-297160-tqw9vx2b	147	19	viruses	virus	NNS
cord-297160-tqw9vx2b	147	20	.	.	.
cord-297160-tqw9vx2b	148	1	Further	further	JJ
cord-297160-tqw9vx2b	148	2	research	research	NN
cord-297160-tqw9vx2b	148	3	will	will	MD
cord-297160-tqw9vx2b	148	4	be	be	VB
cord-297160-tqw9vx2b	148	5	required	require	VBN
cord-297160-tqw9vx2b	148	6	to	to	TO
cord-297160-tqw9vx2b	148	7	investigate	investigate	VB
cord-297160-tqw9vx2b	148	8	repeatability	repeatability	NN
cord-297160-tqw9vx2b	148	9	and	and	CC
cord-297160-tqw9vx2b	148	10	reproducibility	reproducibility	NN
cord-297160-tqw9vx2b	148	11	of	of	IN
cord-297160-tqw9vx2b	148	12	these	these	DT
cord-297160-tqw9vx2b	148	13	methods	method	NNS
cord-297160-tqw9vx2b	148	14	for	for	IN
cord-297160-tqw9vx2b	148	15	virus	virus	NN
cord-297160-tqw9vx2b	148	16	titrations	titration	NNS
cord-297160-tqw9vx2b	148	17	.	.	.
cord-297160-tqw9vx2b	149	1	Coronavirus	Coronavirus	NNP
cord-297160-tqw9vx2b	149	2	avian	avian	JJ
cord-297160-tqw9vx2b	149	3	infectious	infectious	JJ
cord-297160-tqw9vx2b	149	4	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	149	5	virus	virus	NN
cord-297160-tqw9vx2b	149	6	Detection	detection	NN
cord-297160-tqw9vx2b	149	7	of	of	IN
cord-297160-tqw9vx2b	149	8	infectious	infectious	JJ
cord-297160-tqw9vx2b	149	9	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	149	10	virus	virus	NN
cord-297160-tqw9vx2b	149	11	Infectious	infectious	JJ
cord-297160-tqw9vx2b	149	12	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	149	13	virus	virus	NN
cord-297160-tqw9vx2b	149	14	variants	variant	NNS
cord-297160-tqw9vx2b	149	15	:	:	:
cord-297160-tqw9vx2b	149	16	a	a	DT
cord-297160-tqw9vx2b	149	17	review	review	NN
cord-297160-tqw9vx2b	149	18	of	of	IN
cord-297160-tqw9vx2b	149	19	the	the	DT
cord-297160-tqw9vx2b	149	20	history	history	NN
cord-297160-tqw9vx2b	149	21	,	,	,
cord-297160-tqw9vx2b	149	22	current	current	JJ
cord-297160-tqw9vx2b	149	23	situation	situation	NN
cord-297160-tqw9vx2b	149	24	and	and	CC
cord-297160-tqw9vx2b	149	25	control	control	NN
cord-297160-tqw9vx2b	149	26	measures	measure	NNS
cord-297160-tqw9vx2b	149	27	Titration	titration	NN
cord-297160-tqw9vx2b	149	28	of	of	IN
cord-297160-tqw9vx2b	149	29	avian	avian	JJ
cord-297160-tqw9vx2b	149	30	infectious	infectious	JJ
cord-297160-tqw9vx2b	149	31	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	149	32	virus	virus	NN
cord-297160-tqw9vx2b	149	33	in	in	IN
cord-297160-tqw9vx2b	149	34	the	the	DT
cord-297160-tqw9vx2b	149	35	tissues	tissue	NNS
cord-297160-tqw9vx2b	149	36	of	of	IN
cord-297160-tqw9vx2b	149	37	experimentally	experimentally	RB
cord-297160-tqw9vx2b	149	38	infected	infect	VBN
cord-297160-tqw9vx2b	149	39	chickens	chicken	NNS
cord-297160-tqw9vx2b	150	1	The	the	DT
cord-297160-tqw9vx2b	150	2	Spearman	Spearman	NNP
cord-297160-tqw9vx2b	150	3	-	-	HYPH
cord-297160-tqw9vx2b	150	4	Kärber	Kärber	NNP
cord-297160-tqw9vx2b	150	5	method	method	NN
cord-297160-tqw9vx2b	150	6	A	a	DT
cord-297160-tqw9vx2b	150	7	colorimetric	colorimetric	JJ
cord-297160-tqw9vx2b	150	8	assay	assay	NN
cord-297160-tqw9vx2b	150	9	for	for	IN
cord-297160-tqw9vx2b	150	10	viral	viral	JJ
cord-297160-tqw9vx2b	150	11	agents	agent	NNS
cord-297160-tqw9vx2b	150	12	that	that	WDT
cord-297160-tqw9vx2b	150	13	produce	produce	VBP
cord-297160-tqw9vx2b	150	14	cytopathic	cytopathic	JJ
cord-297160-tqw9vx2b	150	15	effects	effect	NNS
cord-297160-tqw9vx2b	150	16	Quantitative	quantitative	JJ
cord-297160-tqw9vx2b	150	17	analysis	analysis	NN
cord-297160-tqw9vx2b	150	18	of	of	IN
cord-297160-tqw9vx2b	150	19	Epstein	Epstein	NNP
cord-297160-tqw9vx2b	150	20	-	-	HYPH
cord-297160-tqw9vx2b	150	21	Barr	Barr	NNP
cord-297160-tqw9vx2b	150	22	virus	virus	NN
cord-297160-tqw9vx2b	150	23	load	load	NN
cord-297160-tqw9vx2b	150	24	by	by	IN
cord-297160-tqw9vx2b	150	25	using	use	VBG
cord-297160-tqw9vx2b	150	26	a	a	DT
cord-297160-tqw9vx2b	150	27	real	real	JJ
cord-297160-tqw9vx2b	150	28	-	-	HYPH
cord-297160-tqw9vx2b	150	29	time	time	NN
cord-297160-tqw9vx2b	150	30	PCR	PCR	NNP
cord-297160-tqw9vx2b	150	31	assay	assay	NN
cord-297160-tqw9vx2b	150	32	Development	Development	NNP
cord-297160-tqw9vx2b	150	33	,	,	,
cord-297160-tqw9vx2b	150	34	evaluation	evaluation	NN
cord-297160-tqw9vx2b	150	35	,	,	,
cord-297160-tqw9vx2b	150	36	and	and	CC
cord-297160-tqw9vx2b	150	37	standardization	standardization	NN
cord-297160-tqw9vx2b	150	38	of	of	IN
cord-297160-tqw9vx2b	150	39	a	a	DT
cord-297160-tqw9vx2b	150	40	real	real	JJ
cord-297160-tqw9vx2b	150	41	-	-	HYPH
cord-297160-tqw9vx2b	150	42	time	time	NN
cord-297160-tqw9vx2b	150	43	TaqMan	TaqMan	NNP
cord-297160-tqw9vx2b	150	44	reverse	reverse	NN
cord-297160-tqw9vx2b	150	45	transcription	transcription	NN
cord-297160-tqw9vx2b	150	46	-	-	HYPH
cord-297160-tqw9vx2b	150	47	PCR	pcr	NN
cord-297160-tqw9vx2b	150	48	assay	assay	NN
cord-297160-tqw9vx2b	150	49	for	for	IN
cord-297160-tqw9vx2b	150	50	quantification	quantification	NN
cord-297160-tqw9vx2b	150	51	of	of	IN
cord-297160-tqw9vx2b	150	52	hepatitis	hepatitis	NNP
cord-297160-tqw9vx2b	150	53	A	A	NNP
cord-297160-tqw9vx2b	150	54	virus	virus	NN
cord-297160-tqw9vx2b	150	55	in	in	IN
cord-297160-tqw9vx2b	150	56	clinical	clinical	JJ
cord-297160-tqw9vx2b	150	57	and	and	CC
cord-297160-tqw9vx2b	150	58	shellfish	shellfish	NN
cord-297160-tqw9vx2b	150	59	samples	sample	NNS
cord-297160-tqw9vx2b	150	60	Attenuation	attenuation	NN
cord-297160-tqw9vx2b	150	61	,	,	,
cord-297160-tqw9vx2b	150	62	safety	safety	NN
cord-297160-tqw9vx2b	150	63	,	,	,
cord-297160-tqw9vx2b	150	64	and	and	CC
cord-297160-tqw9vx2b	150	65	efficacy	efficacy	NN
cord-297160-tqw9vx2b	150	66	of	of	IN
cord-297160-tqw9vx2b	150	67	in	in	IN
cord-297160-tqw9vx2b	150	68	infectious	infectious	JJ
cord-297160-tqw9vx2b	150	69	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	150	70	virus	virus	NN
cord-297160-tqw9vx2b	150	71	GA98	GA98	NNP
cord-297160-tqw9vx2b	150	72	serotype	serotype	NN
cord-297160-tqw9vx2b	150	73	vaccine	vaccine	NN
cord-297160-tqw9vx2b	150	74	Immunity	immunity	NN
cord-297160-tqw9vx2b	150	75	and	and	CC
cord-297160-tqw9vx2b	150	76	cross	cross	NN
cord-297160-tqw9vx2b	150	77	-	-	NN
cord-297160-tqw9vx2b	150	78	protection	protection	NN
cord-297160-tqw9vx2b	150	79	to	to	IN
cord-297160-tqw9vx2b	150	80	nephritis	nephritis	NN
cord-297160-tqw9vx2b	150	81	produced	produce	VBN
cord-297160-tqw9vx2b	150	82	by	by	IN
cord-297160-tqw9vx2b	150	83	Australian	australian	JJ
cord-297160-tqw9vx2b	150	84	infectious	infectious	JJ
cord-297160-tqw9vx2b	150	85	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	150	86	viruses	virus	NNS
cord-297160-tqw9vx2b	150	87	used	use	VBN
cord-297160-tqw9vx2b	150	88	as	as	IN
cord-297160-tqw9vx2b	150	89	vaccines	vaccine	NNS
cord-297160-tqw9vx2b	150	90	Influenza	Influenza	NNP
cord-297160-tqw9vx2b	150	91	virus	virus	NN
cord-297160-tqw9vx2b	150	92	titration	titration	NN
cord-297160-tqw9vx2b	150	93	,	,	,
cord-297160-tqw9vx2b	150	94	antigenic	antigenic	JJ
cord-297160-tqw9vx2b	150	95	characterization	characterization	NN
cord-297160-tqw9vx2b	150	96	,	,	,
cord-297160-tqw9vx2b	150	97	and	and	CC
cord-297160-tqw9vx2b	150	98	serological	serological	JJ
cord-297160-tqw9vx2b	150	99	methods	method	NNS
cord-297160-tqw9vx2b	150	100	for	for	IN
cord-297160-tqw9vx2b	150	101	antibody	antibody	NN
cord-297160-tqw9vx2b	150	102	detection	detection	NN
cord-297160-tqw9vx2b	150	103	Molecular	molecular	JJ
cord-297160-tqw9vx2b	150	104	epidemiology	epidemiology	NN
cord-297160-tqw9vx2b	150	105	of	of	IN
cord-297160-tqw9vx2b	150	106	infectious	infectious	JJ
cord-297160-tqw9vx2b	150	107	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	150	108	virus	virus	NN
cord-297160-tqw9vx2b	150	109	in	in	IN
cord-297160-tqw9vx2b	150	110	The	the	DT
cord-297160-tqw9vx2b	150	111	Netherlands	Netherlands	NNP
cord-297160-tqw9vx2b	150	112	S1	s1	NN
cord-297160-tqw9vx2b	150	113	gene	gene	NN
cord-297160-tqw9vx2b	150	114	sequence	sequence	NN
cord-297160-tqw9vx2b	150	115	heteronegeneity	heteronegeneity	NN
cord-297160-tqw9vx2b	150	116	of	of	IN
cord-297160-tqw9vx2b	150	117	a	a	DT
cord-297160-tqw9vx2b	150	118	pathogenic	pathogenic	JJ
cord-297160-tqw9vx2b	150	119	infectious	infectious	JJ
cord-297160-tqw9vx2b	150	120	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	150	121	virus	virus	NN
cord-297160-tqw9vx2b	150	122	strain	strain	NN
cord-297160-tqw9vx2b	150	123	and	and	CC
cord-297160-tqw9vx2b	150	124	its	-PRON-	PRP$
cord-297160-tqw9vx2b	150	125	embryo	embryo	NN
cord-297160-tqw9vx2b	150	126	-	-	HYPH
cord-297160-tqw9vx2b	150	127	passaged	passage	VBN
cord-297160-tqw9vx2b	150	128	,	,	,
cord-297160-tqw9vx2b	150	129	attenuated	attenuated	JJ
cord-297160-tqw9vx2b	150	130	derivatives	derivative	NNS
cord-297160-tqw9vx2b	150	131	Observations	observation	NNS
cord-297160-tqw9vx2b	150	132	on	on	IN
cord-297160-tqw9vx2b	150	133	the	the	DT
cord-297160-tqw9vx2b	150	134	effects	effect	NNS
cord-297160-tqw9vx2b	150	135	of	of	IN
cord-297160-tqw9vx2b	150	136	the	the	DT
cord-297160-tqw9vx2b	150	137	H52	H52	NNP
cord-297160-tqw9vx2b	150	138	and	and	CC
cord-297160-tqw9vx2b	150	139	H120	H120	NNP
cord-297160-tqw9vx2b	150	140	vaccine	vaccine	NN
cord-297160-tqw9vx2b	150	141	strains	strain	NNS
cord-297160-tqw9vx2b	150	142	of	of	IN
cord-297160-tqw9vx2b	150	143	the	the	DT
cord-297160-tqw9vx2b	150	144	infectious	infectious	JJ
cord-297160-tqw9vx2b	150	145	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	150	146	virus	virus	NN
cord-297160-tqw9vx2b	150	147	in	in	IN
cord-297160-tqw9vx2b	150	148	the	the	DT
cord-297160-tqw9vx2b	150	149	domestic	domestic	JJ
cord-297160-tqw9vx2b	150	150	fowl	fowl	NN
cord-297160-tqw9vx2b	151	1	A	a	DT
cord-297160-tqw9vx2b	151	2	comparison	comparison	NN
cord-297160-tqw9vx2b	151	3	of	of	IN
cord-297160-tqw9vx2b	151	4	titration	titration	NN
cord-297160-tqw9vx2b	151	5	methods	method	NNS
cord-297160-tqw9vx2b	151	6	for	for	IN
cord-297160-tqw9vx2b	151	7	live	live	JJ
cord-297160-tqw9vx2b	151	8	avian	avian	JJ
cord-297160-tqw9vx2b	151	9	encephalomyelitis	encephalomyelitis	NN
cord-297160-tqw9vx2b	151	10	virus	virus	NN
cord-297160-tqw9vx2b	151	11	vaccines	vaccine	NNS
cord-297160-tqw9vx2b	151	12	Type	Type	NNP
cord-297160-tqw9vx2b	151	13	A	a	DT
cord-297160-tqw9vx2b	151	14	influenza	influenza	NN
cord-297160-tqw9vx2b	151	15	detection	detection	NN
cord-297160-tqw9vx2b	151	16	and	and	CC
cord-297160-tqw9vx2b	151	17	quantitation	quantitation	NN
cord-297160-tqw9vx2b	151	18	by	by	IN
cord-297160-tqw9vx2b	151	19	real	real	JJ
cord-297160-tqw9vx2b	151	20	-	-	HYPH
cord-297160-tqw9vx2b	151	21	time	time	NN
cord-297160-tqw9vx2b	151	22	reverse	reverse	NN
cord-297160-tqw9vx2b	151	23	transcriptase	transcriptase	NN
cord-297160-tqw9vx2b	151	24	PCR	PCR	NNP
cord-297160-tqw9vx2b	151	25	Rapid	Rapid	NNP
cord-297160-tqw9vx2b	151	26	retrovirus	retrovirus	NN
cord-297160-tqw9vx2b	151	27	titration	titration	NN
cord-297160-tqw9vx2b	151	28	using	use	VBG
cord-297160-tqw9vx2b	151	29	competitive	competitive	JJ
cord-297160-tqw9vx2b	151	30	polymerase	polymerase	NN
cord-297160-tqw9vx2b	151	31	chain	chain	NN
cord-297160-tqw9vx2b	151	32	reaction	reaction	NN
cord-297160-tqw9vx2b	151	33	Pathogenicity	Pathogenicity	NNP
cord-297160-tqw9vx2b	151	34	of	of	IN
cord-297160-tqw9vx2b	151	35	a	a	DT
cord-297160-tqw9vx2b	151	36	QX	QX	NNP
cord-297160-tqw9vx2b	151	37	strain	strain	NN
cord-297160-tqw9vx2b	151	38	of	of	IN
cord-297160-tqw9vx2b	151	39	infectious	infectious	JJ
cord-297160-tqw9vx2b	151	40	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	151	41	virus	virus	NN
cord-297160-tqw9vx2b	151	42	in	in	IN
cord-297160-tqw9vx2b	151	43	specific	specific	JJ
cord-297160-tqw9vx2b	151	44	pathogen	pathogen	NN
cord-297160-tqw9vx2b	151	45	free	free	JJ
cord-297160-tqw9vx2b	151	46	and	and	CC
cord-297160-tqw9vx2b	151	47	commercial	commercial	JJ
cord-297160-tqw9vx2b	151	48	boiler	boiler	NN
cord-297160-tqw9vx2b	151	49	chickens	chicken	NNS
cord-297160-tqw9vx2b	151	50	and	and	CC
cord-297160-tqw9vx2b	151	51	evaluation	evaluation	NN
cord-297160-tqw9vx2b	151	52	of	of	IN
cord-297160-tqw9vx2b	151	53	protection	protection	NN
cord-297160-tqw9vx2b	151	54	induced	induce	VBN
cord-297160-tqw9vx2b	151	55	by	by	IN
cord-297160-tqw9vx2b	151	56	a	a	DT
cord-297160-tqw9vx2b	151	57	vaccination	vaccination	NN
cord-297160-tqw9vx2b	151	58	program	program	NN
cord-297160-tqw9vx2b	151	59	based	base	VBN
cord-297160-tqw9vx2b	151	60	on	on	IN
cord-297160-tqw9vx2b	151	61	the	the	DT
cord-297160-tqw9vx2b	151	62	Ma5	ma5	NN
cord-297160-tqw9vx2b	151	63	and	and	CC
cord-297160-tqw9vx2b	151	64	4/91	4/91	CD
cord-297160-tqw9vx2b	151	65	serotypes	serotypes	NN
cord-297160-tqw9vx2b	151	66	Simultaneous	simultaneous	JJ
cord-297160-tqw9vx2b	151	67	detection	detection	NN
cord-297160-tqw9vx2b	151	68	of	of	IN
cord-297160-tqw9vx2b	151	69	influenza	influenza	NN
cord-297160-tqw9vx2b	151	70	viruses	virus	NNS
cord-297160-tqw9vx2b	151	71	A	A	NNP
cord-297160-tqw9vx2b	151	72	and	and	CC
cord-297160-tqw9vx2b	151	73	B	b	NN
cord-297160-tqw9vx2b	151	74	using	use	VBG
cord-297160-tqw9vx2b	151	75	real	real	JJ
cord-297160-tqw9vx2b	151	76	-	-	HYPH
cord-297160-tqw9vx2b	151	77	time	time	NN
cord-297160-tqw9vx2b	151	78	quantitative	quantitative	JJ
cord-297160-tqw9vx2b	151	79	PCR	PCR	NNP
cord-297160-tqw9vx2b	151	80	A	a	DT
cord-297160-tqw9vx2b	151	81	reverse	reverse	JJ
cord-297160-tqw9vx2b	151	82	transcriptase	transcriptase	NN
cord-297160-tqw9vx2b	151	83	-	-	HYPH
cord-297160-tqw9vx2b	151	84	polymerase	polymerase	NN
cord-297160-tqw9vx2b	151	85	chain	chain	NN
cord-297160-tqw9vx2b	151	86	reaction	reaction	NN
cord-297160-tqw9vx2b	151	87	survey	survey	NN
cord-297160-tqw9vx2b	151	88	of	of	IN
cord-297160-tqw9vx2b	151	89	infectious	infectious	JJ
cord-297160-tqw9vx2b	151	90	bronchitis	bronchitis	NN
cord-297160-tqw9vx2b	151	91	virus	virus	NN
cord-297160-tqw9vx2b	151	92	genotypes	genotype	NNS
cord-297160-tqw9vx2b	151	93	in	in	IN
cord-297160-tqw9vx2b	151	94	Western	Western	NNP
cord-297160-tqw9vx2b	151	95	Europe	Europe	NNP
cord-297160-tqw9vx2b	151	96	from	from	IN
cord-297160-tqw9vx2b	151	97	Infectivity	infectivity	NN
cord-297160-tqw9vx2b	151	98	titration	titration	NN
cord-297160-tqw9vx2b	151	99	of	of	IN
cord-297160-tqw9vx2b	151	100	the	the	DT
cord-297160-tqw9vx2b	151	101	fast	fast	RB
cord-297160-tqw9vx2b	151	102	-	-	HYPH
cord-297160-tqw9vx2b	151	103	replicating	replicate	VBG
cord-297160-tqw9vx2b	151	104	and	and	CC
cord-297160-tqw9vx2b	151	105	cytopathic	cytopathic	JJ
cord-297160-tqw9vx2b	151	106	hepatitis	hepatitis	NN
cord-297160-tqw9vx2b	151	107	A	a	DT
cord-297160-tqw9vx2b	151	108	virus	virus	NN
cord-297160-tqw9vx2b	151	109	strain	strain	VBP
cord-297160-tqw9vx2b	151	110	HM175A.	HM175A.	NNP
cord-297160-tqw9vx2b	151	111	2	2	CD
cord-297160-tqw9vx2b	151	112	by	by	IN
cord-297160-tqw9vx2b	151	113	an	an	DT
cord-297160-tqw9vx2b	151	114	in	in	IN
cord-297160-tqw9vx2b	151	115	situ	situ	JJ
cord-297160-tqw9vx2b	151	116	enzyme	enzyme	NN
cord-297160-tqw9vx2b	151	117	immunoassay	immunoassay	NN
cord-297160-tqw9vx2b	151	118	Development	Development	NNP
cord-297160-tqw9vx2b	151	119	of	of	IN
cord-297160-tqw9vx2b	151	120	an	an	DT
cord-297160-tqw9vx2b	151	121	improved	improved	JJ
cord-297160-tqw9vx2b	151	122	microneutralization	microneutralization	NN
cord-297160-tqw9vx2b	151	123	assay	assay	NN
cord-297160-tqw9vx2b	151	124	for	for	IN
cord-297160-tqw9vx2b	151	125	respiratory	respiratory	JJ
cord-297160-tqw9vx2b	151	126	syncytial	syncytial	JJ
cord-297160-tqw9vx2b	151	127	virus	virus	NN
cord-297160-tqw9vx2b	151	128	by	by	IN
cord-297160-tqw9vx2b	151	129	automated	automate	VBN
cord-297160-tqw9vx2b	151	130	plaque	plaque	NN
cord-297160-tqw9vx2b	151	131	counting	count	VBG
cord-297160-tqw9vx2b	151	132	using	use	VBG
cord-297160-tqw9vx2b	151	133	imaging	image	VBG
cord-297160-tqw9vx2b	151	134	analysis	analysis	NN