id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-317307-q5mgue5z	Terlizzi, Maria Elena	Quantitative RT Real Time PCR and indirect immunofluorescence for the detection of human parainfluenza virus 1, 2, 3	2009-05-13		.txt	text/plain	3257	151	52	For evaluating IFA sensitivity, 10-fold dilutions (ranging from 10 2 to 10 −2 TCID 50 /200 l) of titrated virus were obtained and different variables, such as days of incubation (2-4 days) and primary monoclonal antibody dilutions (MAb; 1:40-1:80-1:160 in albumin supplemented with PBS 1%), were examined. Different concentrations of TCID 50 (ranged from 10 2 to 10 −5 /reaction) were amplified by the RT Real Time Qt-PCRs in order to compare sensitivity and specificity of the two diagnostic approaches. For the RT Real Time Qt-PCRs, the optimal parameters in obtaining the lowest detection limit with a high specificity resulted in the following concentrations: both primers 0.9 mM and probe 0.25 mM for HPIV-1 and HPIV-2; forward primer 1 mM and reverse 0.9 mM, and probe 0.25 mM for HPIV-3 amplification. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza A and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4	./cache/cord-317307-q5mgue5z.txt	./txt/cord-317307-q5mgue5z.txt