id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-297160-tqw9vx2b	Geerligs, H.J.	The use of RT-PCR for determination of separate end-points for the strains IB H120 and IB D274 in titration of the combination vaccine Poulvac IB(®) primer	2013-07-01		.txt	text/plain	2860	159	58	After the incubation period of seven days standard practice is for the embryos to be taken from each egg and examined visually for IB specific lesions; these readings are used to determine an end-point in viral titrations. In order to determine end-points in the titration for each of the two strains, we collected the allantoic fluids from each egg after the incubation period and tested these for the presence of IB H120 and IB D274 by a strain specific reverse phase PCR. We used PCR for detection of two different IB virus strains in the allantoic fluids from all the individual eggs in a titration of a live IB combination vaccine. Based on the results of the PCR we determined whether an allantoic fluid was positive for one or both of the viruses or not, and as such we were able to define a separate endpoint in the live virus titration for each of the two strains.	./cache/cord-297160-tqw9vx2b.txt	./txt/cord-297160-tqw9vx2b.txt