id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281999-jc4ckqy7	Chen, Yu T.	Proteasome inhibition reduces avian reovirus replication and apoptosis induction in cultured cells	2008-05-02		.txt	text/plain	3326	194	49	The interplay between avian reovirus (ARV) replication and apoptosis and proteasome pathway was studied in cultured cells. In the present study, attempts were made to explore the role of proteasome inhibition in ARV infectivity and the mechanisms involved in the proteasome inhibitor suppression of ARV replication and apoptosis induction in cultured cells. Using the proteasome inhibitor MG132 to inhibit the cellular proteasome pathway, it was found that MG132 could reduce ARV-induced apoptosis, cytopathic effect (CPE), virus titer, and protein expression. The results indicated that the expression of A, C, and NS was reduced significantly in BHK-21cells either pre-incubated 30 min before infection or 2 h.p.i. with MG132 (Fig. 2D) , suggesting that the ubiquitin-proteasome system is not involved in virus internalization. In conclusion, it was shown that proteasome inhibitor reduces ARV replication through inhibition of viral RNA transcription and protein synthesis, thus preventing ARV-induced apoptosis.	./cache/cord-281999-jc4ckqy7.txt	./txt/cord-281999-jc4ckqy7.txt