id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-276503-bh7uugwy	She, Rosemary C.	Flow cytometric detection and serotyping of enterovirus for the clinical laboratory	2009-09-04		.txt	text/plain	2946	146	42	Primary rhesus monkey kidney (PMK) cells were inoculated with several enterovirus serotypes and stained with enterovirus-specific antibodies for flow cytometry and indirect fluorescence antibody testing (IFA). Previous studies have focused on using flow cytometry to quantitate poliovirus infection in neuronal cells (Daley et al., 2005) , characterize enterovirus binding to host cell surfaces (Freistadt and Eberle, 2006; Mbida et al., 1991; Triantafilou et al., 2001) , confirm cytomegalovirus infection of tissue culture cells with a genetically engineered fluorescence reporter system (Kung et al., 2000) , and serotype human immunodeficiency virus type 1 (Zolla-Pazner et al., 1995) . In this study, flow cytometry proved to be a sensitive method for detecting fluorescently stained enterovirus-infected cells. False positives did not occur by flow cytometric analysis in that infected cells were negative after staining with isotype control antibodies and antibodies directed against other enterovirus serotypes.	./cache/cord-276503-bh7uugwy.txt	./txt/cord-276503-bh7uugwy.txt