id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-276368-c9e93h0u	Hosmillo, Myra D.T.	Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses	2010-06-15		.txt	text/plain	4787	217	60	Currently, methods such as real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) have not yet been developed for the rapid detection and quantitation of bovine (BToV) and porcine (PToV) toroviruses. Table 1 List of primers used for conventional RT-PCR, nested PCR and SYBR Green real-time RT-PCR assay for the detection and quantitation of bovine and porcine toroviruses in the fecal specimens from diarrheic calves and piglets. The present study developed, optimized and validated a SYBR Green real-time RT-PCR assay using a universal primer pair for the detection and quantitation of both BToVs and PToVs in archived stool samples. The efficacy and reliability of the SYBR Green real-time RT-PCR assay for the detection and quantitation of BToV and PToV in the 121 bovine and 86 porcine stool samples were evaluated. In conclusion, a rapid, sensitive, specific and reproducible onestep SYBR Green real-time RT-PCR was developed for the detection and quantitation of BToV and PToV in bovine and porcine stool samples.	./cache/cord-276368-c9e93h0u.txt	./txt/cord-276368-c9e93h0u.txt