id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-274954-06c3ymc3	Huang, Yu-Liang	Development of a reverse transcription multiplex real-time PCR for the detection and genotyping of classical swine fever virus	2009-05-04		.txt	text/plain	5727	274	61	title: Development of a reverse transcription multiplex real-time PCR for the detection and genotyping of classical swine fever virus A reverse transcription multiplex real-time PCR (RT-MRT-PCR) was developed for rapid detection and genotyping of classical swine fever virus (CSFV). For the analytical sensitivity experiment, 100 samples of 14 CSFV genotype 1 strains and 86 samples from CSFV outbreak farms were all detected as CSFV-positive by RT-MRT-PCR, and the genotype results were consistent with the results of sequencing from a previous study. 10-fold serially diluted samples of C-strain, Q90-278, and 83-19 strains were determined to be CSFV-positive by viral isolation, RT-PCR, RT-nPCR, and RT-MRT-PCR. a A total of 169 clinical samples was detected for the presence of CSFV and the results included and used to evaluate agreement among viral isolation, RT-PCR, RT-nPCR, and RT-MRT-PCR. It was shown that the sensitivity of RT-MRT-PCR is comparable to those of RT-nPCR and viral isolation, and higher than RT-PCR for the samples of CSFV diluted serially (Table 4 ).	./cache/cord-274954-06c3ymc3.txt	./txt/cord-274954-06c3ymc3.txt