id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-273074-k8m917i4	Fu, Chao-Yang	Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens	2005-12-01		.txt	text/plain	1662	91	50	title: Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and neutralization test results showed that the IgY obtained was of a high purity and had a strong reactive activity with a neutralization titer of 1:640. In this study, we have successfully immunized specific pathogen-free (SPF) chickens, and then purified a high-titer anti-SARS coronavirus yolk immunoglobulin (IgY) with neutralizing activity against SARS coronavirus. The activity of IgY in sera and yolks diluted at 1:200 in phosphate buffer saline (PBS) from immunized animals was assessed using an indirect ELISA assay as described previously (Huang et al., 2005) (Fig. 1) . The development of high-titer anti-SARS coronavirus IgY described in this study would appear to have potential as a new anti-SARS biological product for passive immunization, as it effectively neutralized the SARS coronavirus.	./cache/cord-273074-k8m917i4.txt	./txt/cord-273074-k8m917i4.txt