id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-270421-ytrkob0h	Chen, Pan	A rapid and quantitative assay for measuring neutralizing antibodies of Coxsackievirus B3	2016-03-04		.txt	text/plain	4718	249	49	The CVB3 pseudovirus system was used for quantifying neutralizing antibody (NtAb) levels of 720 human serum samples and showed superior specificity and sensitivity comparing traditional cytopathic effect (CPE) assay. For the lack of CVB3 national antibody standard, we used a mouse serum collected from mice immunized with formalin inactivated CVB3 virus 112 strain and quantified its neutralizing activity against CVB3 (Nancy)-luc pseudovirus in duplicate on the same plate in six independent tests. We next used this pseudovirus assay to measure CVB3 neutralizing antibodies titers in serum samples collected from health adults (18-65 years old). In summary, we established a single round infection system of CVB3 and developed an in vitro assay for detecting neutralizing antibodies in clinical serum samples, and it was a superior surrogate of the assays using wild type viruses including traditional CPE assay and enzyme-linked immunosorbent spot assay.	./cache/cord-270421-ytrkob0h.txt	./txt/cord-270421-ytrkob0h.txt