id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-265634-7n4cvgs4	Dhar, Arun K.	Quantitative assay for measuring the Taura syndrome virus and yellow head virus load in shrimp by real-time RT-PCR using SYBR Green chemistry	2002-03-26		.txt	text/plain	5186	278	61	title: Quantitative assay for measuring the Taura syndrome virus and yellow head virus load in shrimp by real-time RT-PCR using SYBR Green chemistry The current diagnostic methods for TSV and YHV include bioassay using indicator hosts, monitoring clinical signs, histopathology, dot blot, in situ hybridization using virus specific gene probe, immunohistochemistry and by the polymerase chain reaction (PCR) (Lightner and Redman, 1998) . SYBR Green RT-PCR was performed in a 96 well plate using 1 ml of each of the cDNA dilutions for TSV and YHV detection along with EF-1a and b-actin controls following the reaction parameters as described above. The analytical sensitivity of SYBR Green PCR was determined by using a serial dilution of TSV and YHV plasmid DNA as template for amplification. The SYBR Green RT-PCR was not only highly sensitive but also very specific for detecting TSV, YHV and the internal control genes, EF-1a and b-actin.	./cache/cord-265634-7n4cvgs4.txt	./txt/cord-265634-7n4cvgs4.txt