id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-256355-muskjaw3	Black, Elizabeth M	A rapid RT-PCR method to differentiate six established genotypes of rabies and rabies-related viruses using TaqMan™ technology	2002-05-14		.txt	text/plain	4298	221	53	A rapid and sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) assay incorporating TaqMan™ probes has been developed that can distinguish among the six established rabies and rabies-related virus genotypes. TaqMan™ probes were designed and validated against 106 rabies and rabies-related virus isolates, one isolate of the Australian bat Lyssaviruses (genotype 7), and 18 other non-rabies viruses important in the veterinary field. By combining RT-PCR with TaqMan™ genotyping probes suspect rabies virus isolates can be identified in a single closed tube system that prevents potential PCR-product carry over contamination. A hemi-nested reverse transcriptase PCR (hnRT-PCR) assay that uses a cocktail of primers capable of detecting the six established genotypes of rabies and rabies-related viruses has been described (Heaton et al., 1997) . Using the sequence data from a number of isolates from each of the rabies and rabies-related virus genotypes, TaqMan™ probes were designed to distinguish the six established genotypes of rabies and rabies-related viruses.	./cache/cord-256355-muskjaw3.txt	./txt/cord-256355-muskjaw3.txt