id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007648-tm0hn0hz	Mockett, A.P.Adrian	Envelope proteins of avian infectious bronchitis virus: Purification and biological properties	2002-12-20		.txt	text/plain	2217	140	56	Immunoadsorbents, made with monoclonal antibodies, were used to purify the spike and membrane proteins of infectious bronchitis virus (IBV). A second inoculation of virus at 42 days induced an anamnestic antibody response to the spike and membrane proteins and also for the neutralizing antibodies. The objectives of this work were to produce immunoadsorbents using monoclonal antibodies which have been prepared previously (Mockett et al., 1984) and to purify the virus-coded proteins of the viral envelope in a single step in relatively large amounts. In addition the sequential humoral antibody response of chickens after IBV infection has been studied using the purified viral proteins and whole virus in ELISAs and compared to the results using the neutralization test. Thc chicken, about 10 days after an IBV infection, has antibodies to both the spike and membrane proteins in its serum but only very low concentrations of neutralizing antibodies.	./cache/cord-007648-tm0hn0hz.txt	./txt/cord-007648-tm0hn0hz.txt