id author title date pages extension mime words sentences flesch summary cache txt cord-282293-pdhjl508 Park, Wan Beom Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak 2016-01-14 .txt text/plain 1575 90 57 title: Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV. On May 27, 2015, he was unknowingly exposed to the index case (designated as patient number 14 by Korea Ministry of Health and Welfare) of the hospital outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) at emergency department of a hospital (10) . For full-length genome sequencing of the virus isolate (MERS-CoV Hu/KOR/SNU1_035/2015), Vero cell monolayer showing cytopathic effects was harvested and used for RNA extraction. A previous study about S gene of MERS-CoV reported from Korea showed that a culture isolate from patient number 002 contained two nonsynonymous variants (S137R and V530L) (14) . ./cache/cord-282293-pdhjl508.txt ./txt/cord-282293-pdhjl508.txt