id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-324701-7vjdlt1v	Dahlmann, Franziska	Analysis of Ebola Virus Entry Into Macrophages	2015-10-01		.txt	text/plain	5322	273	49	Lectins, TAM receptor tyrosine kinases (Tyro3, Axl, Mer), T cell immunoglobulin and mucin domain (TIM) proteins, integrins, and Niemann-Pick C1 (NPC1) have been reported to promote entry of ebolaviruses into certain cellular systems. Here, we show that mannose-specific lectins, TIM-1 and Axl augment entry into certain cell lines but do not contribute to Ebola virus (EBOV)-glycoprotein (GP)–driven transduction of macrophages. To confirm and extend these observations, we compared the ability of lectins, TIM proteins, and the TAM family member Axl to augment EBOV-GP-driven entry into 293T cells, which can be efficiently transfected and are highly susceptible to transduction mediated by ebolavirus GPs [11] . Quantitative RT-PCR revealed that less Axl and TIM-1 mRNA is produced in MDMs compared with susceptible cell lines, and siRNA-knock-down showed that expression of these factors is dispensable for GP-driven entry into MDMs. In contrast, Mer but not Axl expression was required for efficient GPdriven transduction and EBOV infection of MDMs. Similarly, Mer but not Axl or Tyro3 was found to be essential for efficient phagocytosis of apoptotic cells by macrophages [46] , indicating that Mer is the TAM receptor kinase active in macrophages.	./cache/cord-324701-7vjdlt1v.txt	./txt/cord-324701-7vjdlt1v.txt