id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-293579-w5sub348	Che, Xiao-yan	Antigenic Cross-Reactivity between Severe Acute Respiratory Syndrome—Associated Coronavirus and Human Coronaviruses 229E and OC43	2005-06-15		.txt	text/plain	2564	115	49	By use of Western blot analysis, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between severe acute respiratory syndrome (SARS)—associated coronavirus (SARS-CoV) and 2 HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In the present study, we cloned the nucleocapsid genes of SARS-CoV, HCoV-229E, and HCoV-OC43 and produced specific animal antisera to determine if the nucleocapsid protein is responsible for the observed antigenic cross-reactivity. The serum samples from patients with SARS had antibody responses to SARS-CoV as well as to HCoV-229E and HCoV-OC43 when nucleocapsid proteins were used in the Western blot analysis and when CoV-infected cells were used in the IFA. Furthermore, antibodies to SARS-CoV could be detected in only 1 serum sample from a healthy donor by either IFA or nucleocapsid protein-based Western blot analysis, even though patients with SARS had antibodies to HCoV-229E and HCoV-OC43.	./cache/cord-293579-w5sub348.txt	./txt/cord-293579-w5sub348.txt